Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052767.t1 | XP_003604609.1 | 84.4 | 186 | 29 | 0 | 1 | 186 | 14 | 199 | 6.00E-27 | 131 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052767.t1 | G7JLX3 | 84.4 | 186 | 29 | 0 | 1 | 186 | 14 | 199 | 4.3e-27 | 131.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050670 | MS.gene052767 | 0.803639 | 3.17E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene05886 | 0.809467 | 1.87E-50 | -1.69E-46 |
| MS.gene052767 | MS.gene062767 | 0.804805 | 1.81E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene068612 | 0.819041 | 1.43E-52 | -1.69E-46 |
| MS.gene052767 | MS.gene069859 | 0.811299 | 7.51E-51 | -1.69E-46 |
| MS.gene052767 | MS.gene070108 | 0.824804 | 6.62E-54 | -1.69E-46 |
| MS.gene052767 | MS.gene09945 | 0.840766 | 7.14E-58 | -1.69E-46 |
| MS.gene052767 | MS.gene20025 | 0.803787 | 2.95E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene21142 | 0.818785 | 1.64E-52 | -1.69E-46 |
| MS.gene052767 | MS.gene22295 | 0.813717 | 2.22E-51 | -1.69E-46 |
| MS.gene052767 | MS.gene22296 | 0.819668 | 1.03E-52 | -1.69E-46 |
| MS.gene052767 | MS.gene27514 | 0.800852 | 1.19E-48 | -1.69E-46 |
| MS.gene052767 | MS.gene27912 | 0.814281 | 1.67E-51 | -1.69E-46 |
| MS.gene052767 | MS.gene30306 | 0.800323 | 1.52E-48 | -1.69E-46 |
| MS.gene052767 | MS.gene39429 | 0.8203 | 7.38E-53 | -1.69E-46 |
| MS.gene052767 | MS.gene43917 | 0.816194 | 6.27E-52 | -1.69E-46 |
| MS.gene052767 | MS.gene43919 | 0.818502 | 1.90E-52 | -1.69E-46 |
| MS.gene052767 | MS.gene46239 | 0.82123 | 4.51E-53 | -1.69E-46 |
| MS.gene052767 | MS.gene55270 | 0.80083 | 1.20E-48 | -1.69E-46 |
| MS.gene052767 | MS.gene55349 | 0.81332 | 2.72E-51 | -1.69E-46 |
| MS.gene052767 | MS.gene59730 | 0.812456 | 4.20E-51 | -1.69E-46 |
| MS.gene052767 | MS.gene61579 | 0.805397 | 1.36E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene65859 | 0.831927 | 1.26E-55 | -1.69E-46 |
| MS.gene052767 | MS.gene68315 | 0.800981 | 1.12E-48 | -1.69E-46 |
| MS.gene052767 | MS.gene77433 | 0.802101 | 6.58E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene78436 | 0.817166 | 3.80E-52 | -1.69E-46 |
| MS.gene052767 | MS.gene80354 | 0.809094 | 2.24E-50 | -1.69E-46 |
| MS.gene052767 | MS.gene84406 | 0.80301 | 4.28E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene87271 | 0.844172 | 8.91E-59 | -1.69E-46 |
| MS.gene052767 | MS.gene89914 | 0.804268 | 2.34E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene92773 | 0.804307 | 2.30E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene92871 | 0.801297 | 9.62E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene95885 | 0.809766 | 1.61E-50 | -1.69E-46 |
| MS.gene052767 | MS.gene99806 | 0.834522 | 2.85E-56 | -1.69E-46 |
| MS.gene052767 | MS.gene99854 | 0.809231 | 2.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052767.t1 | MTR_4g015010 | 95.402 | 174 | 8 | 0 | 1 | 174 | 14 | 187 | 1.47e-121 | 345 |
| MS.gene052767.t1 | MTR_7g067000 | 57.803 | 173 | 64 | 5 | 1 | 173 | 1 | 164 | 5.06e-55 | 175 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052767.t1 | AT3G09050 | 49.412 | 170 | 76 | 2 | 8 | 173 | 8 | 171 | 4.42e-46 | 152 |
Find 32 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTCGTCTTACGGATTATT+TGG | 0.131531 | 4.4:+85858758 | MS.gene052767:CDS |
| ATTGGTGAAGATGCTCTTAA+AGG | 0.254433 | 4.4:+85858840 | MS.gene052767:CDS |
| CCAGGTTGTGAATTATTATC+AGG | 0.282631 | 4.4:-85858678 | None:intergenic |
| TCTGAATACTTGACACAGTT+TGG | 0.366087 | 4.4:+85858789 | MS.gene052767:CDS |
| GAGTGAGATGCTAGCATTTG+AGG | 0.367845 | 4.4:+85859917 | MS.gene052767:CDS |
| TTCTTCTTGTGTTGTGGTGG+GGG | 0.398219 | 4.4:-85858605 | None:intergenic |
| TGATTAGAAGGACAGAAGGT+AGG | 0.412522 | 4.4:-85858558 | None:intergenic |
| ATTATTATCAGGTTCATCAT+TGG | 0.413946 | 4.4:-85858667 | None:intergenic |
| TCTTCTTCTTGTGTTGTGGT+GGG | 0.422633 | 4.4:-85858607 | None:intergenic |
| CAAGTTGCGCCAATAGCTCT+TGG | 0.423661 | 4.4:-85858716 | None:intergenic |
| CTTCTTCTTGTGTTGTGGTG+GGG | 0.426287 | 4.4:-85858606 | None:intergenic |
| TTCTTCTTCTTGTGTTGTGG+TGG | 0.434511 | 4.4:-85858608 | None:intergenic |
| AATGGAGATTGAAGGACAAA+TGG | 0.440803 | 4.4:+85859989 | MS.gene052767:CDS |
| TTCTTCTTCTTCTTGTGTTG+TGG | 0.441499 | 4.4:-85858611 | None:intergenic |
| GGACTATTCTTTAAGAACTT+AGG | 0.444508 | 4.4:+85860029 | MS.gene052767:CDS |
| ACTAGACAGAGTAGAGTTTG+AGG | 0.456846 | 4.4:+85859950 | MS.gene052767:CDS |
| TGGCTTTGAACATCACCTCC+AGG | 0.458846 | 4.4:-85858696 | None:intergenic |
| AAAGCTGAAATTGATAAGAT+TGG | 0.462568 | 4.4:+85858822 | MS.gene052767:CDS |
| AAAGTGGTGATGTGATTAGA+AGG | 0.466011 | 4.4:-85858570 | None:intergenic |
| TTTAAGAACTTAGGACAGAA+AGG | 0.478730 | 4.4:+85860038 | MS.gene052767:CDS |
| ATCAGGTTCATCATTGGAGT+TGG | 0.486536 | 4.4:-85858661 | None:intergenic |
| TGTTCAAAGCCAAGAGCTAT+TGG | 0.490338 | 4.4:+85858707 | MS.gene052767:CDS |
| GGAAAGTGAAAGCAAAGTAA+TGG | 0.500197 | 4.4:+85859971 | MS.gene052767:CDS |
| TAGAAGGACAGAAGGTAGGG+TGG | 0.502302 | 4.4:-85858554 | None:intergenic |
| TGAAGATGCTCTTAAAGGAT+TGG | 0.511035 | 4.4:+85858845 | MS.gene052767:CDS |
| ATGGAAGTTGACAGAAATGA+AGG | 0.525154 | 4.4:+85860008 | MS.gene052767:CDS |
| GATGTGATTAGAAGGACAGA+AGG | 0.538979 | 4.4:-85858562 | None:intergenic |
| GATTAGAAGGACAGAAGGTA+GGG | 0.555813 | 4.4:-85858557 | None:intergenic |
| CCTGATAATAATTCACAACC+TGG | 0.556998 | 4.4:+85858678 | MS.gene052767:CDS |
| GGGGTGCAGAGTAGTAAAAG+TGG | 0.557327 | 4.4:-85858586 | None:intergenic |
| AGCAAAGTAATGGAGATTGA+AGG | 0.564863 | 4.4:+85859981 | MS.gene052767:CDS |
| GATAATAATTCACAACCTGG+AGG | 0.700148 | 4.4:+85858681 | MS.gene052767:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATATCTTATAAAAAAATA+TGG | - | chr4.4:85859657-85859676 | None:intergenic | 10.0% |
| !!! | ATACTTTAAAAACTGAATTA+TGG | - | chr4.4:85858995-85859014 | None:intergenic | 15.0% |
| !! | AATCACAAATGAATGTAAAA+GGG | - | chr4.4:85859875-85859894 | None:intergenic | 20.0% |
| !! | AATGTTTACTTATTCTTCAT+TGG | + | chr4.4:85860147-85860166 | MS.gene052767:intron | 20.0% |
| !! | TAATCACAAATGAATGTAAA+AGG | - | chr4.4:85859876-85859895 | None:intergenic | 20.0% |
| !!! | CATCTTTTTTATTTCTTCTT+TGG | - | chr4.4:85860082-85860101 | None:intergenic | 20.0% |
| ! | AAAACTGAATTATGGTTTAC+TGG | - | chr4.4:85858987-85859006 | None:intergenic | 25.0% |
| ! | AAAGCTGAAATTGATAAGAT+TGG | + | chr4.4:85858822-85858841 | MS.gene052767:CDS | 25.0% |
| ! | ATTATTATCAGGTTCATCAT+TGG | - | chr4.4:85858670-85858689 | None:intergenic | 25.0% |
| ! | TACTTTGTACACTTATCATA+TGG | + | chr4.4:85859768-85859787 | MS.gene052767:intron | 25.0% |
| ! | TTAAAGTATAGTTCCTACTA+GGG | - | chr4.4:85859096-85859115 | None:intergenic | 25.0% |
| !! | CTTTTTACATATGGAATAGT+TGG | + | chr4.4:85860171-85860190 | MS.gene052767:intron | 25.0% |
| !! | TTTAAAGTATAGTTCCTACT+AGG | - | chr4.4:85859097-85859116 | None:intergenic | 25.0% |
| !!! | TTCATTGGTCTTTTTACATA+TGG | + | chr4.4:85860162-85860181 | MS.gene052767:intron | 25.0% |
| AAACTGTAGTTTGAATCCTA+GGG | + | chr4.4:85859212-85859231 | MS.gene052767:intron | 30.0% | |
| AGATAAAATGAGAGAGAGAT+TGG | + | chr4.4:85859138-85859157 | MS.gene052767:intron | 30.0% | |
| AGGAAAATGGAAGAAAAACA+AGG | + | chr4.4:85860121-85860140 | MS.gene052767:intron | 30.0% | |
| ATTGGACTATAATGTTAGTC+AGG | + | chr4.4:85859156-85859175 | MS.gene052767:intron | 30.0% | |
| GAAAGATGTAACAACAAATG+AGG | + | chr4.4:85860101-85860120 | MS.gene052767:intron | 30.0% | |
| GTAACAACAAATGAGGAAAA+TGG | + | chr4.4:85860108-85860127 | MS.gene052767:intron | 30.0% | |
| TCATTATGTTAATCCCTAGT+AGG | + | chr4.4:85859080-85859099 | MS.gene052767:intron | 30.0% | |
| TTTAAGAACTTAGGACAGAA+AGG | + | chr4.4:85860038-85860057 | MS.gene052767:CDS | 30.0% | |
| ! | GGACTATTCTTTAAGAACTT+AGG | + | chr4.4:85860029-85860048 | MS.gene052767:CDS | 30.0% |
| ! | TTGAGAATTAGTCTCATAGT+CGG | + | chr4.4:85859290-85859309 | MS.gene052767:intron | 30.0% |
| !! | CATCATTAAGTAGTACTGAT+TGG | + | chr4.4:85860208-85860227 | MS.gene052767:intron | 30.0% |
| !!! | TTTGTTTTGTCAAGATGCTA+GGG | - | chr4.4:85859411-85859430 | None:intergenic | 30.0% |
| AACTGTAGTTTGAATCCTAG+GGG | + | chr4.4:85859213-85859232 | MS.gene052767:intron | 35.0% | |
| AATGGAGATTGAAGGACAAA+TGG | + | chr4.4:85859989-85860008 | MS.gene052767:CDS | 35.0% | |
| ATGGAAGTTGACAGAAATGA+AGG | + | chr4.4:85860008-85860027 | MS.gene052767:CDS | 35.0% | |
| ATTGGTGAAGATGCTCTTAA+AGG | + | chr4.4:85858840-85858859 | MS.gene052767:CDS | 35.0% | |
| CCAGGTTGTGAATTATTATC+AGG | - | chr4.4:85858681-85858700 | None:intergenic | 35.0% | |
| CCTGATAATAATTCACAACC+TGG | + | chr4.4:85858678-85858697 | MS.gene052767:CDS | 35.0% | |
| GAAACTGTAGTTTGAATCCT+AGG | + | chr4.4:85859211-85859230 | MS.gene052767:intron | 35.0% | |
| GACAGAAAAAGTTCGTCTTA+CGG | + | chr4.4:85858749-85858768 | MS.gene052767:CDS | 35.0% | |
| GATAATAATTCACAACCTGG+AGG | + | chr4.4:85858681-85858700 | MS.gene052767:CDS | 35.0% | |
| GGAAAGTGAAAGCAAAGTAA+TGG | + | chr4.4:85859971-85859990 | MS.gene052767:CDS | 35.0% | |
| TCTGAATACTTGACACAGTT+TGG | + | chr4.4:85858789-85858808 | MS.gene052767:CDS | 35.0% | |
| TGAAGATGCTCTTAAAGGAT+TGG | + | chr4.4:85858845-85858864 | MS.gene052767:CDS | 35.0% | |
| TTCTTCTTCTTCTTGTGTTG+TGG | - | chr4.4:85858614-85858633 | None:intergenic | 35.0% | |
| ! | AAAGTGGTGATGTGATTAGA+AGG | - | chr4.4:85858573-85858592 | None:intergenic | 35.0% |
| ! | AGCAAAGTAATGGAGATTGA+AGG | + | chr4.4:85859981-85860000 | MS.gene052767:CDS | 35.0% |
| ! | AGTTCGTCTTACGGATTATT+TGG | + | chr4.4:85858758-85858777 | MS.gene052767:CDS | 35.0% |
| ! | TCTCCTCAAAGTCTCAATTT+TGG | - | chr4.4:85859514-85859533 | None:intergenic | 35.0% |
| !! | CTCAAAGTCTCAATTTTGGT+GGG | - | chr4.4:85859510-85859529 | None:intergenic | 35.0% |
| !! | GCATAAGTACTGCTTTTAAC+AGG | - | chr4.4:85859821-85859840 | None:intergenic | 35.0% |
| !!! | CTTTGGCTTTTGCTTACATT+AGG | - | chr4.4:85860065-85860084 | None:intergenic | 35.0% |
| !!! | CTTTGTTTTGTCAAGATGCT+AGG | - | chr4.4:85859412-85859431 | None:intergenic | 35.0% |
| ACTAGACAGAGTAGAGTTTG+AGG | + | chr4.4:85859950-85859969 | MS.gene052767:CDS | 40.0% | |
| AGCTAGCTAGAATCTATGCA+TGG | - | chr4.4:85858934-85858953 | None:intergenic | 40.0% | |
| AGTTCTTGGAGCTATTCTTG+TGG | + | chr4.4:85860242-85860261 | MS.gene052767:intron | 40.0% | |
| AGTTTCTCAGATACTCCTAC+GGG | - | chr4.4:85859197-85859216 | None:intergenic | 40.0% | |
| ATCAGGTTCATCATTGGAGT+TGG | - | chr4.4:85858664-85858683 | None:intergenic | 40.0% | |
| CAGTTTCTCAGATACTCCTA+CGG | - | chr4.4:85859198-85859217 | None:intergenic | 40.0% | |
| CCACCAAAATTGAGACTTTG+AGG | + | chr4.4:85859508-85859527 | MS.gene052767:intron | 40.0% | |
| CTATGAAGGTTGAGAACGAA+AGG | + | chr4.4:85859242-85859261 | MS.gene052767:intron | 40.0% | |
| CTTTCGTTCTCAACCTTCAT+AGG | - | chr4.4:85859244-85859263 | None:intergenic | 40.0% | |
| GATGCTAGGGACTAATACAA+AGG | - | chr4.4:85859398-85859417 | None:intergenic | 40.0% | |
| GATGTGATTAGAAGGACAGA+AGG | - | chr4.4:85858565-85858584 | None:intergenic | 40.0% | |
| GATTAGAAGGACAGAAGGTA+GGG | - | chr4.4:85858560-85858579 | None:intergenic | 40.0% | |
| GGACTATAATGTTAGTCAGG+TGG | + | chr4.4:85859159-85859178 | MS.gene052767:intron | 40.0% | |
| TCTTCTTCTTGTGTTGTGGT+GGG | - | chr4.4:85858610-85858629 | None:intergenic | 40.0% | |
| TGATTAGAAGGACAGAAGGT+AGG | - | chr4.4:85858561-85858580 | None:intergenic | 40.0% | |
| TGGAAGAAAGTTGCAGTTCT+TGG | + | chr4.4:85860228-85860247 | MS.gene052767:intron | 40.0% | |
| TGTTCAAAGCCAAGAGCTAT+TGG | + | chr4.4:85858707-85858726 | MS.gene052767:CDS | 40.0% | |
| TTCTTCTTCTTGTGTTGTGG+TGG | - | chr4.4:85858611-85858630 | None:intergenic | 40.0% | |
| ! | CCTCAAAGTCTCAATTTTGG+TGG | - | chr4.4:85859511-85859530 | None:intergenic | 40.0% |
| CTTCTTCTTGTGTTGTGGTG+GGG | - | chr4.4:85858609-85858628 | None:intergenic | 45.0% | |
| GAGTGAGATGCTAGCATTTG+AGG | + | chr4.4:85859917-85859936 | MS.gene052767:CDS | 45.0% | |
| GGTCCAAATGACTGAAGTAG+CGG | + | chr4.4:85859311-85859330 | MS.gene052767:intron | 45.0% | |
| GTCCAAATGACTGAAGTAGC+GGG | + | chr4.4:85859312-85859331 | MS.gene052767:intron | 45.0% | |
| GTTTCTCAGATACTCCTACG+GGG | - | chr4.4:85859196-85859215 | None:intergenic | 45.0% | |
| TCCAAATGACTGAAGTAGCG+GGG | + | chr4.4:85859313-85859332 | MS.gene052767:intron | 45.0% | |
| TTCTTCTTGTGTTGTGGTGG+GGG | - | chr4.4:85858608-85858627 | None:intergenic | 45.0% | |
| CAAGTTGCGCCAATAGCTCT+TGG | - | chr4.4:85858719-85858738 | None:intergenic | 50.0% | |
| CCTAGGGGAGTAACCTATGA+AGG | + | chr4.4:85859228-85859247 | MS.gene052767:intron | 50.0% | |
| CCTTCATAGGTTACTCCCCT+AGG | - | chr4.4:85859231-85859250 | None:intergenic | 50.0% | |
| CTCACCGTGATTAGAGCCTT+TGG | - | chr4.4:85859364-85859383 | None:intergenic | 50.0% | |
| GGGGTGCAGAGTAGTAAAAG+TGG | - | chr4.4:85858589-85858608 | None:intergenic | 50.0% | |
| TAGAAGGACAGAAGGTAGGG+TGG | - | chr4.4:85858557-85858576 | None:intergenic | 50.0% | |
| TCCCCGCTACTTCAGTCATT+TGG | - | chr4.4:85859317-85859336 | None:intergenic | 50.0% | |
| TGGAAGCAATTTGACCCCGT+AGG | + | chr4.4:85859179-85859198 | MS.gene052767:intron | 50.0% | |
| ! | GGTGCCAAAGGCTCTAATCA+CGG | + | chr4.4:85859357-85859376 | MS.gene052767:intron | 50.0% |
| ! | TGGACTCATTGTGGTGCCAA+AGG | + | chr4.4:85859345-85859364 | MS.gene052767:intron | 50.0% |
| !! | ATGCCATGGTGGACTCATTG+TGG | + | chr4.4:85859336-85859355 | MS.gene052767:intron | 50.0% |
| !! | TGGCTTTGAACATCACCTCC+AGG | - | chr4.4:85858699-85858718 | None:intergenic | 50.0% |
| GCACCACAATGAGTCCACCA+TGG | - | chr4.4:85859342-85859361 | None:intergenic | 55.0% | |
| ! | AAGTAGCGGGGATGCCATGG+TGG | + | chr4.4:85859325-85859344 | MS.gene052767:intron | 60.0% |
| ! | CTGAAGTAGCGGGGATGCCA+TGG | + | chr4.4:85859322-85859341 | MS.gene052767:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 85858531 | 85860329 | 85858531 | ID=MS.gene052767 |
| chr4.4 | mRNA | 85858531 | 85860329 | 85858531 | ID=MS.gene052767.t1;Parent=MS.gene052767 |
| chr4.4 | exon | 85858531 | 85858884 | 85858531 | ID=MS.gene052767.t1.exon1;Parent=MS.gene052767.t1 |
| chr4.4 | CDS | 85858531 | 85858884 | 85858531 | ID=cds.MS.gene052767.t1;Parent=MS.gene052767.t1 |
| chr4.4 | exon | 85859900 | 85860067 | 85859900 | ID=MS.gene052767.t1.exon2;Parent=MS.gene052767.t1 |
| chr4.4 | CDS | 85859900 | 85860067 | 85859900 | ID=cds.MS.gene052767.t1;Parent=MS.gene052767.t1 |
| chr4.4 | exon | 85860279 | 85860329 | 85860279 | ID=MS.gene052767.t1.exon3;Parent=MS.gene052767.t1 |
| chr4.4 | CDS | 85860279 | 85860329 | 85860279 | ID=cds.MS.gene052767.t1;Parent=MS.gene052767.t1 |
| Gene Sequence |
| Protein sequence |
