AlfalfaGEDB | MS.gene61720

M. sativa cultivar XinJiangDaYe / MS.gene61720

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61720.t1	RHN58490.1	81.1	175	33	0	1	175	91	265	1.90E-72	282

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61720.t1	G7JGU4	81.1	175	33	0	1	175	34	208	1.4e-72	282.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene61720	0.821014	5.06E-53	-1.69E-46
MS.gene049178	MS.gene61720	0.808783	2.61E-50	-1.69E-46
MS.gene049350	MS.gene61720	0.856169	3.86E-62	-1.69E-46
MS.gene049711	MS.gene61720	0.847387	1.19E-59	-1.69E-46
MS.gene050253	MS.gene61720	0.80267	5.03E-49	-1.69E-46
MS.gene05045	MS.gene61720	0.807898	4.04E-50	-1.69E-46
MS.gene050807	MS.gene61720	0.833786	4.37E-56	-1.69E-46
MS.gene050901	MS.gene61720	0.809358	1.97E-50	-1.69E-46
MS.gene051018	MS.gene61720	0.80559	1.24E-49	-1.69E-46
MS.gene051247	MS.gene61720	0.836956	6.90E-57	-1.69E-46
MS.gene051388	MS.gene61720	0.801082	1.06E-48	-1.69E-46
MS.gene05157	MS.gene61720	0.804227	2.39E-49	-1.69E-46
MS.gene051700	MS.gene61720	0.827816	1.27E-54	-1.69E-46
MS.gene051719	MS.gene61720	0.865575	5.35E-65	-1.69E-46
MS.gene051720	MS.gene61720	0.879878	8.69E-70	-1.69E-46
MS.gene051744	MS.gene61720	0.808667	2.77E-50	-1.69E-46
MS.gene052000	MS.gene61720	0.801351	9.38E-49	-1.69E-46
MS.gene052133	MS.gene61720	0.826871	2.14E-54	-1.69E-46
MS.gene052463	MS.gene61720	0.811652	6.29E-51	-1.69E-46
MS.gene052516	MS.gene61720	0.809719	1.65E-50	-1.69E-46
MS.gene052665	MS.gene61720	0.820621	6.23E-53	-1.69E-46
MS.gene052673	MS.gene61720	-0.807405	5.14E-50	-1.69E-46
MS.gene052732	MS.gene61720	0.828327	9.56E-55	-1.69E-46
MS.gene053144	MS.gene61720	0.843405	1.43E-58	-1.69E-46
MS.gene053164	MS.gene61720	0.800013	1.76E-48	-1.69E-46
MS.gene053221	MS.gene61720	0.810979	8.81E-51	-1.69E-46
MS.gene053226	MS.gene61720	0.827427	1.57E-54	-1.69E-46
MS.gene053581	MS.gene61720	0.846437	2.17E-59	-1.69E-46
MS.gene053599	MS.gene61720	0.807887	4.06E-50	-1.69E-46
MS.gene054576	MS.gene61720	0.802417	5.67E-49	-1.69E-46
MS.gene054584	MS.gene61720	0.844694	6.45E-59	-1.69E-46
MS.gene054775	MS.gene61720	0.825745	3.96E-54	-1.69E-46
MS.gene055144	MS.gene61720	0.819042	1.43E-52	-1.69E-46
MS.gene055465	MS.gene61720	0.870505	1.39E-66	-1.69E-46
MS.gene055466	MS.gene61720	0.862241	5.82E-64	-1.69E-46
MS.gene055468	MS.gene61720	0.85135	9.40E-61	-1.69E-46
MS.gene055469	MS.gene61720	0.846828	1.70E-59	-1.69E-46
MS.gene055513	MS.gene61720	0.800892	1.16E-48	-1.69E-46
MS.gene055537	MS.gene61720	0.851649	7.74E-61	-1.69E-46
MS.gene055580	MS.gene61720	-0.830319	3.14E-55	-1.69E-46
MS.gene055891	MS.gene61720	0.804136	2.50E-49	-1.69E-46
MS.gene056005	MS.gene61720	0.807223	5.61E-50	-1.69E-46
MS.gene056043	MS.gene61720	-0.821426	4.06E-53	-1.69E-46
MS.gene056308	MS.gene61720	0.836158	1.10E-56	-1.69E-46
MS.gene056595	MS.gene61720	0.810939	8.98E-51	-1.69E-46
MS.gene057915	MS.gene61720	0.810555	1.09E-50	-1.69E-46
MS.gene058087	MS.gene61720	0.819405	1.18E-52	-1.69E-46
MS.gene058339	MS.gene61720	0.82206	2.90E-53	-1.69E-46
MS.gene058625	MS.gene61720	0.807301	5.41E-50	-1.69E-46
MS.gene058668	MS.gene61720	0.813843	2.08E-51	-1.69E-46
MS.gene058852	MS.gene61720	0.847557	1.07E-59	-1.69E-46
MS.gene059084	MS.gene61720	0.808422	3.12E-50	-1.69E-46
MS.gene059171	MS.gene61720	0.809851	1.54E-50	-1.69E-46
MS.gene060429	MS.gene61720	0.803073	4.15E-49	-1.69E-46
MS.gene060519	MS.gene61720	0.820097	8.21E-53	-1.69E-46
MS.gene060587	MS.gene61720	0.809024	2.32E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61720.t1	MTR_4g009760	97.143	175	5	0	1	175	34	208	1.49e-128	359
MS.gene61720.t1	MTR_2g104230	55.080	187	61	10	1	175	34	209	1.73e-51	164
MS.gene61720.t1	MTR_4g116200	45.545	101	44	4	2	101	35	125	5.80e-16	73.6
MS.gene61720.t1	MTR_2g450720	55.932	59	23	2	2	59	35	91	5.89e-12	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61720.t1	AT1G15760	45.989	187	63	10	3	174	38	201	1.99e-38	130
MS.gene61720.t1	AT1G80520	45.405	185	63	10	1	174	34	191	9.76e-30	108

Find 36 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCATGCTTTGGAGAAATTCA+TGG	0.323501	4.1:-85953242	None:intergenic
GCTAGTAGTAGTAGTTATTA+TGG	0.324043	4.1:+85953465	MS.gene61720:CDS
AAACTTGCAAGAAAAGAAAA+AGG	0.339805	4.1:+85953300	MS.gene61720:CDS
GAGAATTTCTAGCCTGTGTT+TGG	0.345241	4.1:-85953277	None:intergenic
CTATGGAGATTCCCATGCTT+TGG	0.347816	4.1:-85953254	None:intergenic
ATGATCTTTGCCATTGGTCT+TGG	0.351786	4.1:-85953336	None:intergenic
ATCATGATAGCAATGGTTAT+TGG	0.385458	4.1:+85953673	MS.gene61720:CDS
CTACTACTAGCCCTTGTTGT+TGG	0.387585	4.1:-85953453	None:intergenic
ATCTTTGCCATTGGTCTTGG+TGG	0.398986	4.1:-85953333	None:intergenic
CAGCTGCTGTTGAAGATATC+AGG	0.407684	4.1:+85953697	MS.gene61720:CDS
TTCTAGCCTGTGTTTGGCTA+TGG	0.411531	4.1:-85953271	None:intergenic
GCTCCCATGATCTTTGCCAT+TGG	0.442339	4.1:-85953342	None:intergenic
GAGAGGCTGTTGCTTACAAA+TGG	0.465042	4.1:+85953552	MS.gene61720:CDS
AAGACCAATGGCAAAGATCA+TGG	0.469365	4.1:+85953338	MS.gene61720:CDS
GCAGGCATCATTGAAGGACT+AGG	0.479985	4.1:-85953579	None:intergenic
GAAGAGGAATAAGAAGTTGA+AGG	0.494849	4.1:+85953518	MS.gene61720:CDS
TAACTACATAATGAGAAAGT+TGG	0.500944	4.1:+85953392	MS.gene61720:CDS
GCAAGAGCAGGCATCATTGA+AGG	0.509469	4.1:-85953585	None:intergenic
TTTCATGAAGGAAAGATGAA+AGG	0.538904	4.1:+85953645	MS.gene61720:CDS
CCATGAATTTCTCCAAAGCA+TGG	0.539592	4.1:+85953242	MS.gene61720:CDS
AGACCAATGGCAAAGATCAT+GGG	0.562788	4.1:+85953339	MS.gene61720:CDS
GAGGAATAAGAAGTTGAAGG+TGG	0.566010	4.1:+85953521	MS.gene61720:CDS
CATGAATTTCTCCAAAGCAT+GGG	0.566734	4.1:+85953243	MS.gene61720:CDS
TTGTTGTAGTTCCAACAACA+AGG	0.568940	4.1:+85953442	MS.gene61720:CDS
CAGGCATCATTGAAGGACTA+GGG	0.582193	4.1:-85953578	None:intergenic
GAGACAACCACCAAGACCAA+TGG	0.584520	4.1:+85953326	MS.gene61720:CDS
TCATGAAAATGATAAACCAT+AGG	0.596177	4.1:-85953630	None:intergenic
GGAAGAGTTCTGTTGTGAAG+AGG	0.606689	4.1:+85953502	MS.gene61720:CDS
AAACTATCAAGAGCAAGAGC+AGG	0.606845	4.1:-85953597	None:intergenic
CTGCTGTTGAAGATATCAGG+TGG	0.617099	4.1:+85953700	MS.gene61720:CDS
GGAGATGATCATGATAGCAA+TGG	0.631701	4.1:+85953666	MS.gene61720:CDS
TGCTGTTGAAGATATCAGGT+GGG	0.636800	4.1:+85953701	MS.gene61720:CDS
ATTATGGAACAAGATCATCA+TGG	0.658028	4.1:+85953481	MS.gene61720:CDS
GAATCTCCATAGCCAAACAC+AGG	0.676222	4.1:+85953265	MS.gene61720:CDS
TGTTGTAGTTCCAACAACAA+GGG	0.688058	4.1:+85953443	MS.gene61720:CDS
TGAAGGTGGATAAACAAGAG+AGG	0.697861	4.1:+85953535	MS.gene61720:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAACTTGCAAGAAAAGAAAA+AGG	+	chr4.1:85953300-85953319	MS.gene61720:CDS	25.0%
!	TAACTACATAATGAGAAAGT+TGG	+	chr4.1:85953392-85953411	MS.gene61720:CDS	25.0%
!	TCATGAAAATGATAAACCAT+AGG	-	chr4.1:85953633-85953652	None:intergenic	25.0%
!!	ATGGTTTATCATTTTCATGA+AGG	+	chr4.1:85953633-85953652	MS.gene61720:CDS	25.0%
!!!	AAAAAGACAAAAAAGTGCTT+AGG	+	chr4.1:85953369-85953388	MS.gene61720:CDS	25.0%
	ATCATGATAGCAATGGTTAT+TGG	+	chr4.1:85953673-85953692	MS.gene61720:CDS	30.0%
	ATTATGGAACAAGATCATCA+TGG	+	chr4.1:85953481-85953500	MS.gene61720:CDS	30.0%
	GCTAGTAGTAGTAGTTATTA+TGG	+	chr4.1:85953465-85953484	MS.gene61720:CDS	30.0%
	TTTCATGAAGGAAAGATGAA+AGG	+	chr4.1:85953645-85953664	MS.gene61720:CDS	30.0%
!	TAGTTTTTGTAGTACTCCTA+TGG	+	chr4.1:85953614-85953633	MS.gene61720:CDS	30.0%
	CATGAATTTCTCCAAAGCAT+GGG	+	chr4.1:85953243-85953262	MS.gene61720:CDS	35.0%
	GAAGAGGAATAAGAAGTTGA+AGG	+	chr4.1:85953518-85953537	MS.gene61720:CDS	35.0%
	TGTTGTAGTTCCAACAACAA+GGG	+	chr4.1:85953443-85953462	MS.gene61720:CDS	35.0%
	TTGTTGTAGTTCCAACAACA+AGG	+	chr4.1:85953442-85953461	MS.gene61720:CDS	35.0%
	AAACTATCAAGAGCAAGAGC+AGG	-	chr4.1:85953600-85953619	None:intergenic	40.0%
	AAGACCAATGGCAAAGATCA+TGG	+	chr4.1:85953338-85953357	MS.gene61720:CDS	40.0%
	AGACCAATGGCAAAGATCAT+GGG	+	chr4.1:85953339-85953358	MS.gene61720:CDS	40.0%
	CCATGAATTTCTCCAAAGCA+TGG	+	chr4.1:85953242-85953261	MS.gene61720:CDS	40.0%
	GAGGAATAAGAAGTTGAAGG+TGG	+	chr4.1:85953521-85953540	MS.gene61720:CDS	40.0%
	GGAGATGATCATGATAGCAA+TGG	+	chr4.1:85953666-85953685	MS.gene61720:CDS	40.0%
	TGAAGGTGGATAAACAAGAG+AGG	+	chr4.1:85953535-85953554	MS.gene61720:CDS	40.0%
	TGCTGTTGAAGATATCAGGT+GGG	+	chr4.1:85953701-85953720	MS.gene61720:CDS	40.0%
!	ATGATCTTTGCCATTGGTCT+TGG	-	chr4.1:85953339-85953358	None:intergenic	40.0%
!!	CCATGCTTTGGAGAAATTCA+TGG	-	chr4.1:85953245-85953264	None:intergenic	40.0%
!!	GAGAATTTCTAGCCTGTGTT+TGG	-	chr4.1:85953280-85953299	None:intergenic	40.0%
	CAGCTGCTGTTGAAGATATC+AGG	+	chr4.1:85953697-85953716	MS.gene61720:CDS	45.0%
	CAGGCATCATTGAAGGACTA+GGG	-	chr4.1:85953581-85953600	None:intergenic	45.0%
	CTATGGAGATTCCCATGCTT+TGG	-	chr4.1:85953257-85953276	None:intergenic	45.0%
	CTGCTGTTGAAGATATCAGG+TGG	+	chr4.1:85953700-85953719	MS.gene61720:CDS	45.0%
	GAATCTCCATAGCCAAACAC+AGG	+	chr4.1:85953265-85953284	MS.gene61720:CDS	45.0%
	GGAAGAGTTCTGTTGTGAAG+AGG	+	chr4.1:85953502-85953521	MS.gene61720:CDS	45.0%
	TTCTAGCCTGTGTTTGGCTA+TGG	-	chr4.1:85953274-85953293	None:intergenic	45.0%
!	CTACTACTAGCCCTTGTTGT+TGG	-	chr4.1:85953456-85953475	None:intergenic	45.0%
!	GAGAGGCTGTTGCTTACAAA+TGG	+	chr4.1:85953552-85953571	MS.gene61720:CDS	45.0%
!!	ATCTTTGCCATTGGTCTTGG+TGG	-	chr4.1:85953336-85953355	None:intergenic	45.0%
	GAGACAACCACCAAGACCAA+TGG	+	chr4.1:85953326-85953345	MS.gene61720:CDS	50.0%
	GCAAGAGCAGGCATCATTGA+AGG	-	chr4.1:85953588-85953607	None:intergenic	50.0%
	GCAGGCATCATTGAAGGACT+AGG	-	chr4.1:85953582-85953601	None:intergenic	50.0%
	GCTCCCATGATCTTTGCCAT+TGG	-	chr4.1:85953345-85953364	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	85953228	85953755	85953228	ID=MS.gene61720
chr4.1	mRNA	85953228	85953755	85953228	ID=MS.gene61720.t1;Parent=MS.gene61720
chr4.1	exon	85953228	85953755	85953228	ID=MS.gene61720.t1.exon1;Parent=MS.gene61720.t1
chr4.1	CDS	85953228	85953755	85953228	ID=cds.MS.gene61720.t1;Parent=MS.gene61720.t1

Gene Sequence

>MS.gene61720

ATGGTTTACTTCAACCATGAATTTCTCCAAAGCATGGGAATCTCCATAGCCAAACACAGGCTAGAAATTCTCAAACTTGCAAGAAAAGAAAAAGGAAAGAGACAACCACCAAGACCAATGGCAAAGATCATGGGAGCAATCAAAAAGACAAAAAAGTGCTTAGGTAACTACATAATGAGAAAGTTGGTTACATGTGAAGAATCAAATTCAGCACTTGTTGTAGTTCCAACAACAAGGGCTAGTAGTAGTAGTTATTATGGAACAAGATCATCATGGAAGAGTTCTGTTGTGAAGAGGAATAAGAAGTTGAAGGTGGATAAACAAGAGAGGCTGTTGCTTACAAATGGAAGCCCTAGTCCTTCAATGATGCCTGCTCTTGCTCTTGATAGTTTTTGTAGTACTCCTATGGTTTATCATTTTCATGAAGGAAAGATGAAAGGAGATGATCATGATAGCAATGGTTATTGGTCAGCTGCTGTTGAAGATATCAGGTGGGATACTATGTTCCAAGATCTAAAACCAAACTAA

Protein sequence

>MS.gene61720.t1

MVYFNHEFLQSMGISIAKHRLEILKLARKEKGKRQPPRPMAKIMGAIKKTKKCLGNYIMRKLVTCEESNSALVVVPTTRASSSSYYGTRSSWKSSVVKRNKKLKVDKQERLLLTNGSPSPSMMPALALDSFCSTPMVYHFHEGKMKGDDHDSNGYWSAAVEDIRWDTMFQDLKPN