AlfalfaGEDB | MS.gene62561

M. sativa cultivar XinJiangDaYe / MS.gene62561

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62561.t1	XP_004508161.1	98.3	60	1	0	1	60	1	60	2.80E-23	117.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62561.t1	Q9M7X7	85.0	60	9	0	1	60	1	60	1.9e-20	99.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62561.t1	I3SZB6	98.3	60	1	0	1	60	1	60	2.1e-23	117.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049387	MS.gene62561	0.81335	2.68E-51	-1.69E-46
MS.gene050134	MS.gene62561	0.84556	3.76E-59	-1.69E-46
MS.gene050791	MS.gene62561	0.807381	5.20E-50	-1.69E-46
MS.gene051904	MS.gene62561	0.809775	1.60E-50	-1.69E-46
MS.gene052664	MS.gene62561	0.818051	2.40E-52	-1.69E-46
MS.gene052666	MS.gene62561	0.84313	1.69E-58	-1.69E-46
MS.gene053224	MS.gene62561	0.8274	1.60E-54	-1.69E-46
MS.gene057272	MS.gene62561	0.840918	6.51E-58	-1.69E-46
MS.gene057641	MS.gene62561	0.858491	7.94E-63	-1.69E-46
MS.gene057643	MS.gene62561	0.874811	5.04E-68	-1.69E-46
MS.gene059340	MS.gene62561	0.824046	9.98E-54	-1.69E-46
MS.gene060432	MS.gene62561	0.933617	1.43E-95	-1.69E-46
MS.gene060483	MS.gene62561	0.818731	1.68E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene043645	MS.gene62561	PPI
MS.gene62561	MS.gene043595	PPI
MS.gene043593	MS.gene62561	PPI
MS.gene064353	MS.gene62561	PPI
MS.gene62561	MS.gene035825	PPI
MS.gene035826	MS.gene62561	PPI
MS.gene064351	MS.gene62561	PPI
MS.gene035828	MS.gene62561	PPI
MS.gene62561	MS.gene73093	PPI
MS.gene99872	MS.gene62561	PPI
MS.gene043643	MS.gene62561	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62561.t1	MTR_4g121877	98.333	60	1	0	1	60	1	60	7.50e-38	119
MS.gene62561.t1	MTR_4g121870	95.000	60	3	0	1	60	137	196	1.29e-36	120

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62561.t1	AT3G06680	85.000	60	9	1	60	23	82	2.96e-31	103
MS.gene62561.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene62561.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene62561.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene62561.t1	AT3G06680	85.000	60	9	1	60	1	60	6.50e-31	102
MS.gene62561.t1	AT3G06680	85.000	60	9	1	60	1	60	6.50e-31	102

Find 13 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTGTGGCGGTGCCTCTT+TGG	0.285127	7.3:+69022246	None:intergenic
TATGCTTCCGTGCGTACCTC+TGG	0.442911	7.3:+69022092	None:intergenic
ACCGCCACACTTCCACCAAA+GGG	0.460737	7.3:-69022236	MS.gene62561:CDS
CGCTCACAACCAGTCTTACA+AGG	0.508995	7.3:-69022292	MS.gene62561:CDS
ATGATGAATACATACCCCTT+TGG	0.517971	7.3:+69022221	None:intergenic
CACCGCCACACTTCCACCAA+AGG	0.519273	7.3:-69022237	MS.gene62561:CDS
ATGAATACATACCCCTTTGG+TGG	0.547453	7.3:+69022224	None:intergenic
ATGGCATCAAGAAGCCAAAG+AGG	0.617235	7.3:-69022260	MS.gene62561:CDS
ATACCCCTTTGGTGGAAGTG+TGG	0.622392	7.3:+69022232	None:intergenic
CCCCTTTGGTGGAAGTGTGG+CGG	0.635065	7.3:+69022235	None:intergenic
CCGCCACACTTCCACCAAAG+GGG	0.674155	7.3:-69022235	MS.gene62561:intron
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	7.3:+69022293	None:intergenic
TGAGGAACCAGAGGTACGCA+CGG	0.675026	7.3:-69022099	MS.gene62561:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGTGATTGATTACTGTAGA+TGG	-	chr7.3:69022238-69022257	MS.gene62561:CDS	30.0%
	ATGATGAATACATACCCCTT+TGG	+	chr7.3:69022154-69022173	None:intergenic	35.0%
!	AGATGGATCCAAAGTTTTTG+AGG	-	chr7.3:69022255-69022274	MS.gene62561:CDS	35.0%
!	CGGAAGCATAACAAGAAAAA+TGG	-	chr7.3:69022293-69022312	MS.gene62561:CDS	35.0%
!!!	CTTCTTGATGCCATTTTTGT+GGG	+	chr7.3:69022106-69022125	None:intergenic	35.0%
	ATGAATACATACCCCTTTGG+TGG	+	chr7.3:69022151-69022170	None:intergenic	40.0%
	TCTTACAAGGCCCACAAAAA+TGG	-	chr7.3:69022093-69022112	MS.gene62561:CDS	40.0%
!	CTCTGGTTCCTCAAAAACTT+TGG	+	chr7.3:69022266-69022285	None:intergenic	40.0%
!!	CAAAGTTTTTGAGGAACCAG+AGG	-	chr7.3:69022264-69022283	MS.gene62561:CDS	40.0%
!!!	GCTTCTTGATGCCATTTTTG+TGG	+	chr7.3:69022107-69022126	None:intergenic	40.0%
	ATGGCATCAAGAAGCCAAAG+AGG	-	chr7.3:69022112-69022131	MS.gene62561:CDS	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	+	chr7.3:69022082-69022101	None:intergenic	45.0%
!!	TTTTGTGGGCCTTGTAAGAC+TGG	+	chr7.3:69022092-69022111	None:intergenic	45.0%
	ATACCCCTTTGGTGGAAGTG+TGG	+	chr7.3:69022143-69022162	None:intergenic	50.0%
	CGCTCACAACCAGTCTTACA+AGG	-	chr7.3:69022080-69022099	MS.gene62561:CDS	50.0%
	ACCGCCACACTTCCACCAAA+GGG	-	chr7.3:69022136-69022155	MS.gene62561:intron	55.0%
	TATGCTTCCGTGCGTACCTC+TGG	+	chr7.3:69022283-69022302	None:intergenic	55.0%
!	TGAGGAACCAGAGGTACGCA+CGG	-	chr7.3:69022273-69022292	MS.gene62561:CDS	55.0%
	CACCGCCACACTTCCACCAA+AGG	-	chr7.3:69022135-69022154	MS.gene62561:intron	60.0%
	CCGCCACACTTCCACCAAAG+GGG	-	chr7.3:69022137-69022156	MS.gene62561:intron	60.0%
	GAAGTGTGGCGGTGCCTCTT+TGG	+	chr7.3:69022129-69022148	None:intergenic	60.0%
!	CCCCTTTGGTGGAAGTGTGG+CGG	+	chr7.3:69022140-69022159	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	69022057	69022337	69022057	ID=MS.gene62561
chr7.3	mRNA	69022057	69022337	69022057	ID=MS.gene62561.t1;Parent=MS.gene62561
chr7.3	exon	69022236	69022337	69022236	ID=MS.gene62561.t1.exon1;Parent=MS.gene62561.t1
chr7.3	CDS	69022236	69022337	69022236	ID=cds.MS.gene62561.t1;Parent=MS.gene62561.t1
chr7.3	exon	69022057	69022137	69022057	ID=MS.gene62561.t1.exon2;Parent=MS.gene62561.t1
chr7.3	CDS	69022057	69022137	69022057	ID=cds.MS.gene62561.t1;Parent=MS.gene62561.t1

Gene Sequence	>MS.gene62561 ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCCCACAAAAATGGCATCAAGAAGCCAAAGAGGCACCGCCACACTTCCACCAAAGGGGTATGTATTCATCATTCTTCTCTGATTTTTCAAACGATTGTTCTATTTTGTAATGAGATTTTAATTAATTGTTTATTTATTGTGATTGATTACTGTAGATGGATCCAAAGTTTTTGAGGAACCAGAGGTACGCACGGAAGCATAACAAGAAAAATGGTGAAATTGCAGAAGAAGAGTAA
Protein sequence	>MS.gene62561.t1 MAKSKNHTAHNQSYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEIAEEE