Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65951.t1 | XP_003590043.1 | 73.5 | 505 | 3 | 2 | 1 | 374 | 29 | 533 | 4.40E-191 | 677.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65951.t1 | O48639 | 59.6 | 490 | 67 | 3 | 1 | 360 | 30 | 518 | 8.0e-149 | 528.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65951.t1 | O22302 | 73.5 | 505 | 3 | 2 | 1 | 374 | 29 | 533 | 3.2e-191 | 677.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene65951 | -0.801037 | 1.09E-48 | -1.69E-46 |
| MS.gene049549 | MS.gene65951 | 0.830384 | 3.03E-55 | -1.69E-46 |
| MS.gene049634 | MS.gene65951 | 0.817942 | 2.54E-52 | -1.69E-46 |
| MS.gene050095 | MS.gene65951 | 0.826254 | 3.00E-54 | -1.69E-46 |
| MS.gene050096 | MS.gene65951 | 0.830645 | 2.61E-55 | -1.69E-46 |
| MS.gene050380 | MS.gene65951 | 0.801722 | 7.87E-49 | -1.69E-46 |
| MS.gene050647 | MS.gene65951 | 0.810746 | 9.89E-51 | -1.69E-46 |
| MS.gene050720 | MS.gene65951 | 0.815181 | 1.05E-51 | -1.69E-46 |
| MS.gene05104 | MS.gene65951 | 0.805367 | 1.38E-49 | -1.69E-46 |
| MS.gene051158 | MS.gene65951 | 0.815434 | 9.27E-52 | -1.69E-46 |
| MS.gene052211 | MS.gene65951 | 0.846527 | 2.05E-59 | -1.69E-46 |
| MS.gene05240 | MS.gene65951 | 0.821527 | 3.85E-53 | -1.69E-46 |
| MS.gene05244 | MS.gene65951 | 0.801938 | 7.11E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene65951 | 0.849823 | 2.52E-60 | -1.69E-46 |
| MS.gene053031 | MS.gene65951 | 0.81278 | 3.57E-51 | -1.69E-46 |
| MS.gene053033 | MS.gene65951 | 0.805598 | 1.24E-49 | -1.69E-46 |
| MS.gene053403 | MS.gene65951 | 0.862739 | 4.09E-64 | -1.69E-46 |
| MS.gene053406 | MS.gene65951 | 0.935393 | 9.10E-97 | -1.69E-46 |
| MS.gene053420 | MS.gene65951 | 0.801191 | 1.01E-48 | -1.69E-46 |
| MS.gene054146 | MS.gene65951 | 0.84329 | 1.53E-58 | -1.69E-46 |
| MS.gene054290 | MS.gene65951 | 0.802863 | 4.58E-49 | -1.69E-46 |
| MS.gene054742 | MS.gene65951 | 0.818221 | 2.19E-52 | -1.69E-46 |
| MS.gene055124 | MS.gene65951 | 0.809964 | 1.46E-50 | -1.69E-46 |
| MS.gene055240 | MS.gene65951 | 0.832469 | 9.29E-56 | -1.69E-46 |
| MS.gene056218 | MS.gene65951 | 0.812227 | 4.71E-51 | -1.69E-46 |
| MS.gene056748 | MS.gene65951 | 0.811465 | 6.91E-51 | -1.69E-46 |
| MS.gene057261 | MS.gene65951 | 0.812607 | 3.89E-51 | -1.69E-46 |
| MS.gene058117 | MS.gene65951 | 0.800069 | 1.71E-48 | -1.69E-46 |
| MS.gene058171 | MS.gene65951 | 0.802465 | 5.54E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene65951 | -0.803602 | 3.22E-49 | -1.69E-46 |
| MS.gene058603 | MS.gene65951 | 0.833459 | 5.27E-56 | -1.69E-46 |
| MS.gene059892 | MS.gene65951 | 0.82651 | 2.61E-54 | -1.69E-46 |
| MS.gene059902 | MS.gene65951 | -0.80163 | 8.22E-49 | -1.69E-46 |
| MS.gene059944 | MS.gene65951 | 0.803627 | 3.18E-49 | -1.69E-46 |
| MS.gene059955 | MS.gene65951 | 0.815394 | 9.46E-52 | -1.69E-46 |
| MS.gene060087 | MS.gene65951 | 0.815345 | 9.70E-52 | -1.69E-46 |
| MS.gene060593 | MS.gene65951 | 0.810651 | 1.04E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65951.t1 | MTR_1g043290 | 85.475 | 358 | 3 | 1 | 66 | 374 | 176 | 533 | 0.0 | 607 |
| MS.gene65951.t1 | MTR_1g043290 | 100.000 | 67 | 0 | 0 | 1 | 67 | 29 | 95 | 5.37e-38 | 143 |
| MS.gene65951.t1 | MTR_1g043220 | 85.714 | 357 | 2 | 1 | 66 | 373 | 176 | 532 | 0.0 | 606 |
| MS.gene65951.t1 | MTR_1g043220 | 100.000 | 67 | 0 | 0 | 1 | 67 | 29 | 95 | 6.46e-38 | 143 |
| MS.gene65951.t1 | MTR_1g043200 | 81.564 | 358 | 17 | 2 | 66 | 374 | 176 | 533 | 0.0 | 575 |
| MS.gene65951.t1 | MTR_1g043200 | 97.015 | 67 | 2 | 0 | 1 | 67 | 29 | 95 | 5.87e-37 | 140 |
| MS.gene65951.t1 | MTR_1g074930 | 74.716 | 352 | 40 | 1 | 66 | 368 | 176 | 527 | 0.0 | 533 |
| MS.gene65951.t1 | MTR_1g074930 | 85.075 | 67 | 10 | 0 | 1 | 67 | 29 | 95 | 2.42e-31 | 124 |
| MS.gene65951.t1 | MTR_3g082700 | 66.369 | 336 | 63 | 2 | 66 | 352 | 177 | 511 | 3.09e-159 | 458 |
| MS.gene65951.t1 | MTR_3g082700 | 79.104 | 67 | 14 | 0 | 1 | 67 | 30 | 96 | 2.46e-28 | 116 |
| MS.gene65951.t1 | MTR_1g069935 | 63.610 | 349 | 80 | 2 | 66 | 368 | 177 | 524 | 5.88e-154 | 444 |
| MS.gene65951.t1 | MTR_1g069935 | 77.612 | 67 | 15 | 0 | 1 | 67 | 30 | 96 | 2.03e-27 | 113 |
| MS.gene65951.t1 | MTR_7g096880 | 65.231 | 325 | 68 | 2 | 72 | 352 | 183 | 506 | 3.94e-149 | 432 |
| MS.gene65951.t1 | MTR_7g096880 | 77.612 | 67 | 15 | 0 | 1 | 67 | 30 | 96 | 4.34e-27 | 112 |
| MS.gene65951.t1 | MTR_7g096870 | 63.842 | 354 | 81 | 2 | 66 | 373 | 177 | 529 | 7.40e-149 | 431 |
| MS.gene65951.t1 | MTR_7g096870 | 74.627 | 67 | 17 | 0 | 1 | 67 | 30 | 96 | 6.51e-27 | 112 |
| MS.gene65951.t1 | MTR_1g074940 | 63.717 | 339 | 57 | 3 | 66 | 352 | 176 | 500 | 5.55e-142 | 413 |
| MS.gene65951.t1 | MTR_1g074940 | 70.149 | 67 | 19 | 1 | 1 | 67 | 30 | 95 | 4.75e-24 | 103 |
| MS.gene65951.t1 | MTR_1g069930 | 58.235 | 340 | 94 | 3 | 66 | 358 | 177 | 515 | 2.87e-135 | 397 |
| MS.gene65951.t1 | MTR_1g069930 | 65.385 | 78 | 27 | 0 | 1 | 78 | 30 | 107 | 1.15e-24 | 105 |
| MS.gene65951.t1 | MTR_1g028600 | 46.023 | 352 | 130 | 5 | 69 | 373 | 177 | 515 | 1.77e-94 | 292 |
| MS.gene65951.t1 | MTR_1g028600 | 76.119 | 67 | 16 | 0 | 1 | 67 | 27 | 93 | 1.28e-27 | 114 |
| MS.gene65951.t1 | MTR_4g083960 | 45.198 | 354 | 130 | 9 | 66 | 369 | 175 | 514 | 5.92e-91 | 283 |
| MS.gene65951.t1 | MTR_5g068140 | 42.819 | 376 | 134 | 7 | 69 | 373 | 179 | 544 | 8.02e-89 | 278 |
| MS.gene65951.t1 | MTR_5g068140 | 73.134 | 67 | 18 | 0 | 1 | 67 | 29 | 95 | 1.07e-26 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65951.t1 | AT5G43360 | 70.262 | 343 | 53 | 2 | 66 | 360 | 177 | 518 | 6.73e-165 | 472 |
| MS.gene65951.t1 | AT5G43360 | 77.612 | 67 | 15 | 0 | 1 | 67 | 30 | 96 | 3.24e-28 | 115 |
| MS.gene65951.t1 | AT5G43350 | 69.096 | 343 | 57 | 2 | 66 | 360 | 177 | 518 | 1.17e-162 | 466 |
| MS.gene65951.t1 | AT5G43350 | 77.612 | 67 | 15 | 0 | 1 | 67 | 30 | 96 | 2.95e-28 | 115 |
| MS.gene65951.t1 | AT2G32830 | 64.789 | 355 | 74 | 2 | 66 | 370 | 177 | 530 | 2.75e-162 | 466 |
| MS.gene65951.t1 | AT2G32830 | 77.612 | 67 | 15 | 0 | 1 | 67 | 30 | 96 | 2.24e-26 | 110 |
| MS.gene65951.t1 | AT3G54700 | 68.824 | 340 | 60 | 2 | 66 | 360 | 177 | 515 | 6.20e-161 | 462 |
| MS.gene65951.t1 | AT3G54700 | 79.104 | 67 | 14 | 0 | 1 | 67 | 30 | 96 | 9.83e-28 | 114 |
| MS.gene65951.t1 | AT3G54700 | 68.824 | 340 | 60 | 2 | 66 | 360 | 177 | 515 | 6.20e-161 | 462 |
| MS.gene65951.t1 | AT3G54700 | 79.104 | 67 | 14 | 0 | 1 | 67 | 30 | 96 | 9.83e-28 | 114 |
| MS.gene65951.t1 | AT2G38940 | 65.689 | 341 | 67 | 2 | 66 | 357 | 177 | 516 | 9.35e-155 | 446 |
| MS.gene65951.t1 | AT2G38940 | 79.104 | 67 | 14 | 0 | 1 | 67 | 30 | 96 | 1.38e-27 | 114 |
| MS.gene65951.t1 | AT5G43370 | 68.222 | 343 | 60 | 2 | 66 | 360 | 177 | 518 | 3.68e-149 | 432 |
| MS.gene65951.t1 | AT5G43370 | 77.612 | 67 | 15 | 0 | 1 | 67 | 30 | 96 | 3.15e-28 | 115 |
| MS.gene65951.t1 | AT5G43370 | 68.222 | 343 | 60 | 2 | 66 | 360 | 177 | 518 | 3.68e-149 | 432 |
| MS.gene65951.t1 | AT5G43370 | 77.612 | 67 | 15 | 0 | 1 | 67 | 30 | 96 | 3.15e-28 | 115 |
| MS.gene65951.t1 | AT5G43340 | 54.203 | 345 | 96 | 3 | 66 | 362 | 178 | 508 | 6.75e-123 | 364 |
| MS.gene65951.t1 | AT5G43340 | 67.164 | 67 | 22 | 0 | 1 | 67 | 31 | 97 | 4.70e-22 | 97.8 |
| MS.gene65951.t1 | AT1G76430 | 42.363 | 347 | 137 | 10 | 71 | 362 | 175 | 513 | 4.22e-79 | 252 |
| MS.gene65951.t1 | AT1G20860 | 43.438 | 320 | 128 | 7 | 102 | 370 | 200 | 517 | 1.45e-77 | 248 |
| MS.gene65951.t1 | AT1G20860 | 43.438 | 320 | 128 | 7 | 102 | 370 | 252 | 569 | 4.12e-77 | 249 |
| MS.gene65951.t1 | AT4G08895 | 55.714 | 140 | 30 | 1 | 162 | 301 | 23 | 130 | 2.34e-48 | 161 |
| MS.gene65951.t1 | AT4G08878 | 59.375 | 96 | 36 | 2 | 66 | 161 | 159 | 251 | 2.62e-27 | 109 |
| MS.gene65951.t1 | AT4G08878 | 66.667 | 66 | 22 | 0 | 2 | 67 | 13 | 78 | 1.74e-21 | 93.6 |
Find 91 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCTGCATTAACTTATTAT+TGG | 0.168469 | 1.1:+27065543 | MS.gene65951:CDS |
| ACATTCTTCTTTGCTAATTT+TGG | 0.173620 | 1.1:+27066106 | MS.gene65951:CDS |
| GCTGTGTAACGGGCTGTTTC+TGG | 0.206707 | 1.1:-27065578 | None:intergenic |
| GGCAGCTTGTTTCGCGTTCT+TGG | 0.212885 | 1.1:-27065609 | None:intergenic |
| GCAGTACTGTTCCGGGTTAC+TGG | 0.238649 | 1.1:+27065930 | MS.gene65951:CDS |
| TGCGCTGTGCAGTACTGTTC+CGG | 0.249470 | 1.1:+27065922 | MS.gene65951:CDS |
| AATTATTCTTTGGTTGGCTT+GGG | 0.264750 | 1.1:+27065120 | MS.gene65951:CDS |
| TATATTTGATCACAAATATA+AGG | 0.273690 | 1.1:+27065430 | MS.gene65951:CDS |
| GCACTTGGTGGAAGTGTTCC+TGG | 0.285473 | 1.1:-27065050 | None:intergenic |
| GGAGCAATTGTTGGTGCATT+TGG | 0.287811 | 1.1:+27066223 | MS.gene65951:CDS |
| GATCCTACCAAGACCGATAA+AGG | 0.287833 | 1.1:+27066271 | MS.gene65951:CDS |
| TGCCTCAATTTGATTATGTT+TGG | 0.291088 | 1.1:+27065492 | MS.gene65951:CDS |
| CTAGCAGGCCAATTATTCTT+TGG | 0.295996 | 1.1:+27065110 | MS.gene65951:CDS |
| CAAGCCAACCAAAGAATAAT+TGG | 0.296172 | 1.1:-27065118 | None:intergenic |
| AGCAAGGAAGAGAATAGAAT+TGG | 0.305550 | 1.1:+27066061 | MS.gene65951:CDS |
| CAATTATTCTTTGGTTGGCT+TGG | 0.312479 | 1.1:+27065119 | MS.gene65951:CDS |
| TCCCTTGTCACAAAGTTGTT+AGG | 0.314005 | 1.1:+27064993 | MS.gene65951:CDS |
| GTTCCTGGTCTTGTCGGATT+TGG | 0.332250 | 1.1:-27065035 | None:intergenic |
| ACAACAAATGTTGTAGCATT+TGG | 0.334728 | 1.1:-27066130 | None:intergenic |
| GTTATGCACTTTGCTTGTTC+CGG | 0.348633 | 1.1:+27066360 | MS.gene65951:CDS |
| AAAGGGTATCCAACTGGTAT+TGG | 0.365619 | 1.1:+27066289 | MS.gene65951:CDS |
| GCGCTGTGCAGTACTGTTCC+GGG | 0.395138 | 1.1:+27065923 | MS.gene65951:CDS |
| TGGATACCCTTTATCGGTCT+TGG | 0.398885 | 1.1:-27066278 | None:intergenic |
| TCCTTCTTGCTCAGTGGCCT+CGG | 0.404042 | 1.1:-27066437 | None:intergenic |
| CCCTTGTCACAAAGTTGTTA+GGG | 0.410280 | 1.1:+27064994 | MS.gene65951:CDS |
| TCCTTGCTCCAATGATCATA+TGG | 0.411149 | 1.1:-27066046 | None:intergenic |
| TGGAGACTTATTCTTATGTT+CGG | 0.411605 | 1.1:+27065512 | MS.gene65951:CDS |
| AGTGCTCAATCAGCTGTAAC+TGG | 0.412121 | 1.1:+27065068 | MS.gene65951:CDS |
| ACCAGTTGGATACCCTTTAT+CGG | 0.412868 | 1.1:-27066284 | None:intergenic |
| CTCGGTGTGATCAACTTTGT+TGG | 0.421696 | 1.1:+27066334 | MS.gene65951:CDS |
| TCAGCTGCCGCTGGAAAGGC+AGG | 0.422195 | 1.1:+27066202 | MS.gene65951:CDS |
| AGCTGCCGCGGATATGTCTA+AGG | 0.424705 | 1.1:+27065625 | MS.gene65951:CDS |
| AAGGGTATCCAACTGGTATT+GGG | 0.427208 | 1.1:+27066290 | MS.gene65951:CDS |
| GTAACTGGTGTTGCACTTGT+TGG | 0.429691 | 1.1:+27065083 | MS.gene65951:CDS |
| AGAGTTAAGTGGAGAGAATG+AGG | 0.433879 | 1.1:+27066405 | MS.gene65951:CDS |
| TTGCCATACCATATGATCAT+TGG | 0.440480 | 1.1:+27066038 | MS.gene65951:CDS |
| TCGTTTCGCTATTCAAATGA+TGG | 0.440848 | 1.1:+27065982 | MS.gene65951:CDS |
| GGTTGGCTTGGGGACAAACT+TGG | 0.443517 | 1.1:+27065131 | MS.gene65951:CDS |
| CAGGCCAATTATTCTTTGGT+TGG | 0.445254 | 1.1:+27065114 | MS.gene65951:CDS |
| GGCAACAAGAGCTGTGTAAC+GGG | 0.457457 | 1.1:-27065588 | None:intergenic |
| CACGGAATCTCAGCTGCCGC+TGG | 0.459217 | 1.1:+27066193 | MS.gene65951:CDS |
| GGCAGCTGAGATTCCGTGAC+AGG | 0.468128 | 1.1:-27066188 | None:intergenic |
| ACCGATAAAGGGTATCCAAC+TGG | 0.474139 | 1.1:+27066283 | MS.gene65951:CDS |
| AGTTGCGTTTATCGATTACA+TGG | 0.480935 | 1.1:+27065958 | MS.gene65951:CDS |
| GGAAGTGTTCCTGGTCTTGT+CGG | 0.485453 | 1.1:-27065041 | None:intergenic |
| TTCTTAATCCCAATACCAGT+TGG | 0.488319 | 1.1:-27066298 | None:intergenic |
| TCGGTGTGATCAACTTTGTT+GGG | 0.490978 | 1.1:+27066335 | MS.gene65951:CDS |
| GGCACTAACAATGATGCTGC+TGG | 0.492284 | 1.1:-27065473 | None:intergenic |
| GCAAGGAAGAGAATAGAATT+GGG | 0.494872 | 1.1:+27066062 | MS.gene65951:CDS |
| TTTGCTTGTTCCGGAATCAA+AGG | 0.495899 | 1.1:+27066369 | MS.gene65951:CDS |
| AATCTCAGCTGCCGCTGGAA+AGG | 0.496144 | 1.1:+27066198 | MS.gene65951:CDS |
| GCACTTGTTGGAACACTAGC+AGG | 0.499060 | 1.1:+27065095 | MS.gene65951:CDS |
| GTTACAGCTGATTGAGCACT+TGG | 0.502566 | 1.1:-27065065 | None:intergenic |
| ATTATTCTTTGGTTGGCTTG+GGG | 0.503981 | 1.1:+27065121 | MS.gene65951:CDS |
| CAATAATAAGTTAATGCAGC+TGG | 0.504368 | 1.1:-27065542 | None:intergenic |
| ATCCTACCAAGACCGATAAA+GGG | 0.505979 | 1.1:+27066272 | MS.gene65951:CDS |
| TTGCTTGTTCCGGAATCAAA+GGG | 0.506174 | 1.1:+27066370 | MS.gene65951:CDS |
| CGTTTCGCTATTCAAATGAT+GGG | 0.506889 | 1.1:+27065983 | MS.gene65951:CDS |
| CCACTGAGCAAGAAGGATCA+AGG | 0.506925 | 1.1:+27066443 | MS.gene65951:CDS |
| GAACCAAATCCGACAAGACC+AGG | 0.511277 | 1.1:+27065032 | MS.gene65951:CDS |
| TGGAATTGTTGCTTTGATTG+TGG | 0.522228 | 1.1:+27065403 | MS.gene65951:CDS |
| GGAAAGGCAGGAGCAATTGT+TGG | 0.523447 | 1.1:+27066214 | MS.gene65951:CDS |
| AGTGGAGAGAATGAGGGAGA+AGG | 0.543109 | 1.1:+27066412 | MS.gene65951:CDS |
| CTCCAAACATAATCAAATTG+AGG | 0.545090 | 1.1:-27065494 | None:intergenic |
| AGTTGAACTTGAAGTTGAAG+AGG | 0.547307 | 1.1:+27065655 | MS.gene65951:CDS |
| CCCTAACAACTTTGTGACAA+GGG | 0.549878 | 1.1:-27064994 | None:intergenic |
| ACTTGAAGTTGAAGAGGAGA+AGG | 0.556695 | 1.1:+27065661 | MS.gene65951:CDS |
| CCTTGATCCTTCTTGCTCAG+TGG | 0.558886 | 1.1:-27066443 | None:intergenic |
| GGAATCAAAGGGAAAATCGT+TGG | 0.559818 | 1.1:+27066381 | MS.gene65951:CDS |
| TGGCAACAAGAGCTGTGTAA+CGG | 0.571302 | 1.1:-27065589 | None:intergenic |
| GAGTTAAGTGGAGAGAATGA+GGG | 0.575057 | 1.1:+27066406 | MS.gene65951:CDS |
| ACAGCTGATTGAGCACTTGG+TGG | 0.575728 | 1.1:-27065062 | None:intergenic |
| AAGAACTCGCTTATCATGCT+CGG | 0.577140 | 1.1:+27066316 | MS.gene65951:CDS |
| AGGTTAAGGTCAACCTGTCA+CGG | 0.579119 | 1.1:+27066175 | MS.gene65951:CDS |
| GCTCCAATGATCATATGGTA+TGG | 0.582429 | 1.1:-27066041 | None:intergenic |
| CAGGTTGACCTTAACCTAGC+CGG | 0.591908 | 1.1:-27066169 | None:intergenic |
| TGCAATCGGATGGATCCCTC+CGG | 0.592267 | 1.1:+27065841 | MS.gene65951:CDS |
| AGAAATGAATGCAATTCATG+AGG | 0.592937 | 1.1:+27065868 | MS.gene65951:CDS |
| AAATCGTTGGAAGAGTTAAG+TGG | 0.593957 | 1.1:+27066394 | MS.gene65951:CDS |
| TCCCTAACAACTTTGTGACA+AGG | 0.594495 | 1.1:-27064995 | None:intergenic |
| GAATGAGGGAGAAGGTGCCG+AGG | 0.594746 | 1.1:+27066420 | MS.gene65951:CDS |
| CACTGAGCAAGAAGGATCAA+GGG | 0.602834 | 1.1:+27066444 | MS.gene65951:CDS |
| TACCCTTTATCGGTCTTGGT+AGG | 0.605944 | 1.1:-27066274 | None:intergenic |
| ACCATATGATCATTGGAGCA+AGG | 0.612455 | 1.1:+27066045 | MS.gene65951:CDS |
| GCAACTGTGAACCAGTAACC+CGG | 0.616763 | 1.1:-27065941 | None:intergenic |
| GCCGAGGCCACTGAGCAAGA+AGG | 0.621352 | 1.1:+27066436 | MS.gene65951:CDS |
| TACAACATTTGTTGTACCTG+CGG | 0.625308 | 1.1:+27066138 | MS.gene65951:CDS |
| GAACGCGAAACAAGCTGCCG+CGG | 0.638322 | 1.1:+27065613 | MS.gene65951:CDS |
| GTTGCGTTTATCGATTACAT+GGG | 0.662830 | 1.1:+27065959 | MS.gene65951:CDS |
| AATTGCTCCTGCCTTTCCAG+CGG | 0.674047 | 1.1:-27066209 | None:intergenic |
| CAATACCTTAGACATATCCG+CGG | 0.722449 | 1.1:-27065630 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATTTGATCACAAATATA+AGG | + | chr1.1:27065430-27065449 | MS.gene65951:CDS | 15.0% |
| !! | AAAAAATGACTAGTGATAAA+AGG | + | chr1.1:27065687-27065706 | MS.gene65951:intron | 20.0% |
| ! | AGTGATAAAAGGAATAGTTA+TGG | + | chr1.1:27065698-27065717 | MS.gene65951:intron | 25.0% |
| ! | TATGCTAACAAAAAAACTAG+AGG | + | chr1.1:27065329-27065348 | MS.gene65951:intron | 25.0% |
| !! | ACATTCTTCTTTGCTAATTT+TGG | + | chr1.1:27066106-27066125 | MS.gene65951:CDS | 25.0% |
| !! | TAGTCAAAATCTTTTCCAAA+AGG | + | chr1.1:27065808-27065827 | MS.gene65951:intron | 25.0% |
| ACAACAAATGTTGTAGCATT+TGG | - | chr1.1:27066133-27066152 | None:intergenic | 30.0% | |
| AGAAATGAATGCAATTCATG+AGG | + | chr1.1:27065868-27065887 | MS.gene65951:CDS | 30.0% | |
| CAATAATAAGTTAATGCAGC+TGG | - | chr1.1:27065545-27065564 | None:intergenic | 30.0% | |
| CAGCTGCATTAACTTATTAT+TGG | + | chr1.1:27065543-27065562 | MS.gene65951:CDS | 30.0% | |
| CTCCAAACATAATCAAATTG+AGG | - | chr1.1:27065497-27065516 | None:intergenic | 30.0% | |
| TGCCTCAATTTGATTATGTT+TGG | + | chr1.1:27065492-27065511 | MS.gene65951:CDS | 30.0% | |
| TGGAGACTTATTCTTATGTT+CGG | + | chr1.1:27065512-27065531 | MS.gene65951:CDS | 30.0% | |
| TGGTTTAACTCTAATTCTCA+TGG | + | chr1.1:27065169-27065188 | MS.gene65951:intron | 30.0% | |
| TTAACTCTAATTCTCATGGT+GGG | + | chr1.1:27065173-27065192 | MS.gene65951:intron | 30.0% | |
| TTTAACTCTAATTCTCATGG+TGG | + | chr1.1:27065172-27065191 | MS.gene65951:intron | 30.0% | |
| ! | ATTGCATTCATTTCTTTTGC+CGG | - | chr1.1:27065863-27065882 | None:intergenic | 30.0% |
| ! | TTGCACTAAAAATGTCCTTT+TGG | - | chr1.1:27065826-27065845 | None:intergenic | 30.0% |
| !! | AATTATTCTTTGGTTGGCTT+GGG | + | chr1.1:27065120-27065139 | MS.gene65951:CDS | 30.0% |
| !! | ATGTAGTACTTGGTTTTTGT+TGG | + | chr1.1:27065772-27065791 | MS.gene65951:intron | 30.0% |
| !! | CACTTTGTTTCTTTAGGTTT+TGG | + | chr1.1:27065252-27065271 | MS.gene65951:intron | 30.0% |
| !!! | AAGGACATTTTTAGTGCAAT+CGG | + | chr1.1:27065827-27065846 | MS.gene65951:intron | 30.0% |
| AAATCGTTGGAAGAGTTAAG+TGG | + | chr1.1:27066394-27066413 | MS.gene65951:CDS | 35.0% | |
| ACATGATTGTAGCTGAAAGA+GGG | - | chr1.1:27065304-27065323 | None:intergenic | 35.0% | |
| AGCAAGGAAGAGAATAGAAT+TGG | + | chr1.1:27066061-27066080 | MS.gene65951:CDS | 35.0% | |
| AGTTGAACTTGAAGTTGAAG+AGG | + | chr1.1:27065655-27065674 | MS.gene65951:CDS | 35.0% | |
| CAAGCCAACCAAAGAATAAT+TGG | - | chr1.1:27065121-27065140 | None:intergenic | 35.0% | |
| CGTTTCGCTATTCAAATGAT+GGG | + | chr1.1:27065983-27066002 | MS.gene65951:CDS | 35.0% | |
| CTTGGTAGGAAAAAAGTCTA+TGG | + | chr1.1:27065149-27065168 | MS.gene65951:intron | 35.0% | |
| GCAAGGAAGAGAATAGAATT+GGG | + | chr1.1:27066062-27066081 | MS.gene65951:CDS | 35.0% | |
| TCGTTTCGCTATTCAAATGA+TGG | + | chr1.1:27065982-27066001 | MS.gene65951:CDS | 35.0% | |
| TTCTTAATCCCAATACCAGT+TGG | - | chr1.1:27066301-27066320 | None:intergenic | 35.0% | |
| TTGCCATACCATATGATCAT+TGG | + | chr1.1:27066038-27066057 | MS.gene65951:CDS | 35.0% | |
| ! | AACGATTTTCCCTTTGATTC+CGG | - | chr1.1:27066382-27066401 | None:intergenic | 35.0% |
| ! | AGTTGCGTTTATCGATTACA+TGG | + | chr1.1:27065958-27065977 | MS.gene65951:CDS | 35.0% |
| ! | CAATTATTCTTTGGTTGGCT+TGG | + | chr1.1:27065119-27065138 | MS.gene65951:CDS | 35.0% |
| ! | GCTGGATTTTCTTCAAATGT+GGG | - | chr1.1:27065458-27065477 | None:intergenic | 35.0% |
| ! | GTTGCGTTTATCGATTACAT+GGG | + | chr1.1:27065959-27065978 | MS.gene65951:CDS | 35.0% |
| ! | TGCTGGATTTTCTTCAAATG+TGG | - | chr1.1:27065459-27065478 | None:intergenic | 35.0% |
| ! | TGGCAACACTTTGTTTCTTT+AGG | + | chr1.1:27065246-27065265 | MS.gene65951:intron | 35.0% |
| ! | TGTTTGGAACATGTAGTACT+TGG | + | chr1.1:27065762-27065781 | MS.gene65951:intron | 35.0% |
| !! | ATCAAGTCCAAAGAGTGTTA+TGG | + | chr1.1:27065226-27065245 | MS.gene65951:intron | 35.0% |
| !! | ATGCAAGGTTTTGGTATCTT+AGG | + | chr1.1:27065377-27065396 | MS.gene65951:intron | 35.0% |
| !! | ATTATTCTTTGGTTGGCTTG+GGG | + | chr1.1:27065121-27065140 | MS.gene65951:CDS | 35.0% |
| !! | GTTTTTGCTATGCAAGGTTT+TGG | + | chr1.1:27065368-27065387 | MS.gene65951:intron | 35.0% |
| !! | TACAACATTTGTTGTACCTG+CGG | + | chr1.1:27066138-27066157 | MS.gene65951:CDS | 35.0% |
| !! | TGGAATTGTTGCTTTGATTG+TGG | + | chr1.1:27065403-27065422 | MS.gene65951:CDS | 35.0% |
| !!! | ACATTTTTAGTGCAATCGGA+TGG | + | chr1.1:27065831-27065850 | MS.gene65951:CDS | 35.0% |
| !!! | TGTTTCTTTAGGTTTTGGCT+TGG | + | chr1.1:27065257-27065276 | MS.gene65951:intron | 35.0% |
| !!! | TTTAGGTTTTGGCTTGGTTT+TGG | + | chr1.1:27065263-27065282 | MS.gene65951:intron | 35.0% |
| !!! | TTTTGGCTTGGTTTTGGAAT+TGG | + | chr1.1:27065269-27065288 | MS.gene65951:intron | 35.0% |
| AAGAACTCGCTTATCATGCT+CGG | + | chr1.1:27066316-27066335 | MS.gene65951:CDS | 40.0% | |
| ACCAGTTGGATACCCTTTAT+CGG | - | chr1.1:27066287-27066306 | None:intergenic | 40.0% | |
| ACCATATGATCATTGGAGCA+AGG | + | chr1.1:27066045-27066064 | MS.gene65951:CDS | 40.0% | |
| ACTTGAAGTTGAAGAGGAGA+AGG | + | chr1.1:27065661-27065680 | MS.gene65951:CDS | 40.0% | |
| AGAGTTAAGTGGAGAGAATG+AGG | + | chr1.1:27066405-27066424 | MS.gene65951:CDS | 40.0% | |
| AGTGTTGCCATAACACTCTT+TGG | - | chr1.1:27065236-27065255 | None:intergenic | 40.0% | |
| ATCCTACCAAGACCGATAAA+GGG | + | chr1.1:27066272-27066291 | MS.gene65951:CDS | 40.0% | |
| CAATACCTTAGACATATCCG+CGG | - | chr1.1:27065633-27065652 | None:intergenic | 40.0% | |
| CAGGCCAATTATTCTTTGGT+TGG | + | chr1.1:27065114-27065133 | MS.gene65951:CDS | 40.0% | |
| CCCTAACAACTTTGTGACAA+GGG | - | chr1.1:27064997-27065016 | None:intergenic | 40.0% | |
| CCCTTGTCACAAAGTTGTTA+GGG | + | chr1.1:27064994-27065013 | MS.gene65951:CDS | 40.0% | |
| CTAGCAGGCCAATTATTCTT+TGG | + | chr1.1:27065110-27065129 | MS.gene65951:CDS | 40.0% | |
| GACATGATTGTAGCTGAAAG+AGG | - | chr1.1:27065305-27065324 | None:intergenic | 40.0% | |
| GAGTTAAGTGGAGAGAATGA+GGG | + | chr1.1:27066406-27066425 | MS.gene65951:CDS | 40.0% | |
| GCTCCAATGATCATATGGTA+TGG | - | chr1.1:27066044-27066063 | None:intergenic | 40.0% | |
| GGAATCAAAGGGAAAATCGT+TGG | + | chr1.1:27066381-27066400 | MS.gene65951:CDS | 40.0% | |
| GTTATGCACTTTGCTTGTTC+CGG | + | chr1.1:27066360-27066379 | MS.gene65951:CDS | 40.0% | |
| TCCCTAACAACTTTGTGACA+AGG | - | chr1.1:27064998-27065017 | None:intergenic | 40.0% | |
| TCCCTTGTCACAAAGTTGTT+AGG | + | chr1.1:27064993-27065012 | MS.gene65951:CDS | 40.0% | |
| TCCTTGCTCCAATGATCATA+TGG | - | chr1.1:27066049-27066068 | None:intergenic | 40.0% | |
| TCGGTGTGATCAACTTTGTT+GGG | + | chr1.1:27066335-27066354 | MS.gene65951:CDS | 40.0% | |
| TTGCTTGTTCCGGAATCAAA+GGG | + | chr1.1:27066370-27066389 | MS.gene65951:CDS | 40.0% | |
| TTTGCTTGTTCCGGAATCAA+AGG | + | chr1.1:27066369-27066388 | MS.gene65951:CDS | 40.0% | |
| ! | AGATTTTTCCGGCTAGGTTA+AGG | + | chr1.1:27066161-27066180 | MS.gene65951:CDS | 40.0% |
| ! | CATTCATTTCTTTTGCCGGA+GGG | - | chr1.1:27065859-27065878 | None:intergenic | 40.0% |
| !! | AAAGGGTATCCAACTGGTAT+TGG | + | chr1.1:27066289-27066308 | MS.gene65951:CDS | 40.0% |
| !! | AAGGGTATCCAACTGGTATT+GGG | + | chr1.1:27066290-27066309 | MS.gene65951:CDS | 40.0% |
| !! | CAAGGTTTTGGTATCTTAGG+TGG | + | chr1.1:27065380-27065399 | MS.gene65951:intron | 40.0% |
| AAGCAATTCGCGAAACGACA+TGG | + | chr1.1:27065731-27065750 | MS.gene65951:intron | 45.0% | |
| AATTCGCGAAACGACATGGT+TGG | + | chr1.1:27065735-27065754 | MS.gene65951:intron | 45.0% | |
| AGGTTAAGGTCAACCTGTCA+CGG | + | chr1.1:27066175-27066194 | MS.gene65951:CDS | 45.0% | |
| AGTGCTCAATCAGCTGTAAC+TGG | + | chr1.1:27065068-27065087 | MS.gene65951:CDS | 45.0% | |
| ATTCGCGAAACGACATGGTT+GGG | + | chr1.1:27065736-27065755 | MS.gene65951:intron | 45.0% | |
| CACTGAGCAAGAAGGATCAA+GGG | + | chr1.1:27066444-27066463 | MS.gene65951:CDS | 45.0% | |
| GATCCTACCAAGACCGATAA+AGG | + | chr1.1:27066271-27066290 | MS.gene65951:CDS | 45.0% | |
| GGAGCAATTGTTGGTGCATT+TGG | + | chr1.1:27066223-27066242 | MS.gene65951:CDS | 45.0% | |
| GTTACAGCTGATTGAGCACT+TGG | - | chr1.1:27065068-27065087 | None:intergenic | 45.0% | |
| TACCCTTTATCGGTCTTGGT+AGG | - | chr1.1:27066277-27066296 | None:intergenic | 45.0% | |
| TGATCCAAAAGAGAGTCCTG+AGG | - | chr1.1:27065210-27065229 | None:intergenic | 45.0% | |
| TGGATACCCTTTATCGGTCT+TGG | - | chr1.1:27066281-27066300 | None:intergenic | 45.0% | |
| TGGCAACAAGAGCTGTGTAA+CGG | - | chr1.1:27065592-27065611 | None:intergenic | 45.0% | |
| ! | ATGTCGTTTCGCGAATTGCT+TGG | - | chr1.1:27065732-27065751 | None:intergenic | 45.0% |
| ! | CTCGGTGTGATCAACTTTGT+TGG | + | chr1.1:27066334-27066353 | MS.gene65951:CDS | 45.0% |
| ! | GCATTCATTTCTTTTGCCGG+AGG | - | chr1.1:27065860-27065879 | None:intergenic | 45.0% |
| ! | TGTACCTGCGGAGATTTTTC+CGG | + | chr1.1:27066150-27066169 | MS.gene65951:CDS | 45.0% |
| !! | ACCGATAAAGGGTATCCAAC+TGG | + | chr1.1:27066283-27066302 | MS.gene65951:CDS | 45.0% |
| !! | GGTTTTGGTATCTTAGGTGG+TGG | + | chr1.1:27065383-27065402 | MS.gene65951:intron | 45.0% |
| !! | GTAACTGGTGTTGCACTTGT+TGG | + | chr1.1:27065083-27065102 | MS.gene65951:CDS | 45.0% |
| !!! | GCTGCTGTTTTTGCTATGCA+AGG | + | chr1.1:27065362-27065381 | MS.gene65951:intron | 45.0% |
| !!! | TGGCTTGGTTTTGGAATTGG+TGG | + | chr1.1:27065272-27065291 | MS.gene65951:intron | 45.0% |
| AATTGCTCCTGCCTTTCCAG+CGG | - | chr1.1:27066212-27066231 | None:intergenic | 50.0% | |
| ACAGCTGATTGAGCACTTGG+TGG | - | chr1.1:27065065-27065084 | None:intergenic | 50.0% | |
| AGTGGAGAGAATGAGGGAGA+AGG | + | chr1.1:27066412-27066431 | MS.gene65951:CDS | 50.0% | |
| CCACTGAGCAAGAAGGATCA+AGG | + | chr1.1:27066443-27066462 | MS.gene65951:CDS | 50.0% | |
| CCTTGATCCTTCTTGCTCAG+TGG | - | chr1.1:27066446-27066465 | None:intergenic | 50.0% | |
| GAACCAAATCCGACAAGACC+AGG | + | chr1.1:27065032-27065051 | MS.gene65951:CDS | 50.0% | |
| GCAACTGTGAACCAGTAACC+CGG | - | chr1.1:27065944-27065963 | None:intergenic | 50.0% | |
| GGAAAGGCAGGAGCAATTGT+TGG | + | chr1.1:27066214-27066233 | MS.gene65951:CDS | 50.0% | |
| GGCAACAAGAGCTGTGTAAC+GGG | - | chr1.1:27065591-27065610 | None:intergenic | 50.0% | |
| GGCACTAACAATGATGCTGC+TGG | - | chr1.1:27065476-27065495 | None:intergenic | 50.0% | |
| ! | CAGGTTGACCTTAACCTAGC+CGG | - | chr1.1:27066172-27066191 | None:intergenic | 50.0% |
| ! | GCACTTGTTGGAACACTAGC+AGG | + | chr1.1:27065095-27065114 | MS.gene65951:CDS | 50.0% |
| ! | GTTGCCTCAGGACTCTCTTT+TGG | + | chr1.1:27065203-27065222 | MS.gene65951:intron | 50.0% |
| !! | CGACATGGTTGGGCTTTGTT+TGG | + | chr1.1:27065746-27065765 | MS.gene65951:intron | 50.0% |
| !! | GGAAGTGTTCCTGGTCTTGT+CGG | - | chr1.1:27065044-27065063 | None:intergenic | 50.0% |
| !! | GTTCCTGGTCTTGTCGGATT+TGG | - | chr1.1:27065038-27065057 | None:intergenic | 50.0% |
| AATCTCAGCTGCCGCTGGAA+AGG | + | chr1.1:27066198-27066217 | MS.gene65951:CDS | 55.0% | |
| AGCTGCCGCGGATATGTCTA+AGG | + | chr1.1:27065625-27065644 | MS.gene65951:CDS | 55.0% | |
| CTAGCCGGAAAAATCTCCGC+AGG | - | chr1.1:27066157-27066176 | None:intergenic | 55.0% | |
| GCAGTACTGTTCCGGGTTAC+TGG | + | chr1.1:27065930-27065949 | MS.gene65951:CDS | 55.0% | |
| GGCTTGGGGACAAACTTGGT+AGG | + | chr1.1:27065135-27065154 | MS.gene65951:intron | 55.0% | |
| TCCTTCTTGCTCAGTGGCCT+CGG | - | chr1.1:27066440-27066459 | None:intergenic | 55.0% | |
| TGCAATCGGATGGATCCCTC+CGG | + | chr1.1:27065841-27065860 | MS.gene65951:CDS | 55.0% | |
| TGCGCTGTGCAGTACTGTTC+CGG | + | chr1.1:27065922-27065941 | MS.gene65951:CDS | 55.0% | |
| ! | CTGCGGAGATTTTTCCGGCT+AGG | + | chr1.1:27066155-27066174 | MS.gene65951:CDS | 55.0% |
| ! | GGCAGCTTGTTTCGCGTTCT+TGG | - | chr1.1:27065612-27065631 | None:intergenic | 55.0% |
| ! | GTGGGTTGTTCTGTTGCCTC+AGG | + | chr1.1:27065191-27065210 | MS.gene65951:intron | 55.0% |
| !! | GCACTTGGTGGAAGTGTTCC+TGG | - | chr1.1:27065053-27065072 | None:intergenic | 55.0% |
| !! | GCTGTGTAACGGGCTGTTTC+TGG | - | chr1.1:27065581-27065600 | None:intergenic | 55.0% |
| !! | GGTTGGCTTGGGGACAAACT+TGG | + | chr1.1:27065131-27065150 | MS.gene65951:CDS | 55.0% |
| GAACGCGAAACAAGCTGCCG+CGG | + | chr1.1:27065613-27065632 | MS.gene65951:CDS | 60.0% | |
| GCGCTGTGCAGTACTGTTCC+GGG | + | chr1.1:27065923-27065942 | MS.gene65951:CDS | 60.0% | |
| GGCAGCTGAGATTCCGTGAC+AGG | - | chr1.1:27066191-27066210 | None:intergenic | 60.0% | |
| !! | GAATGAGGGAGAAGGTGCCG+AGG | + | chr1.1:27066420-27066439 | MS.gene65951:CDS | 60.0% |
| CACGGAATCTCAGCTGCCGC+TGG | + | chr1.1:27066193-27066212 | MS.gene65951:CDS | 65.0% | |
| GCCGAGGCCACTGAGCAAGA+AGG | + | chr1.1:27066436-27066455 | MS.gene65951:CDS | 65.0% | |
| TCAGCTGCCGCTGGAAAGGC+AGG | + | chr1.1:27066202-27066221 | MS.gene65951:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 27064954 | 27066471 | 27064954 | ID=MS.gene65951 |
| chr1.1 | mRNA | 27064954 | 27066471 | 27064954 | ID=MS.gene65951.t1;Parent=MS.gene65951 |
| chr1.1 | exon | 27064954 | 27065152 | 27064954 | ID=MS.gene65951.t1.exon1;Parent=MS.gene65951.t1 |
| chr1.1 | CDS | 27064954 | 27065152 | 27064954 | ID=cds.MS.gene65951.t1;Parent=MS.gene65951.t1 |
| chr1.1 | exon | 27065399 | 27065682 | 27065399 | ID=MS.gene65951.t1.exon2;Parent=MS.gene65951.t1 |
| chr1.1 | CDS | 27065399 | 27065682 | 27065399 | ID=cds.MS.gene65951.t1;Parent=MS.gene65951.t1 |
| chr1.1 | exon | 27065830 | 27066471 | 27065830 | ID=MS.gene65951.t1.exon3;Parent=MS.gene65951.t1 |
| chr1.1 | CDS | 27065830 | 27066471 | 27065830 | ID=cds.MS.gene65951.t1;Parent=MS.gene65951.t1 |
| Gene Sequence |
| Protein sequence |
