Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66979.t1 | XP_003618966.1 | 97.8 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 2.40E-42 | 181 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66979.t1 | G7KPM7 | 97.8 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 1.7e-42 | 181.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04920 | MS.gene66979 | 0.809367 | 1.96E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene66979 | 0.815362 | 9.61E-52 | -1.69E-46 |
| MS.gene049580 | MS.gene66979 | 0.82098 | 5.15E-53 | -1.69E-46 |
| MS.gene049680 | MS.gene66979 | 0.809112 | 2.22E-50 | -1.69E-46 |
| MS.gene04998 | MS.gene66979 | 0.804027 | 2.63E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene66979 | 0.81262 | 3.87E-51 | -1.69E-46 |
| MS.gene051043 | MS.gene66979 | 0.804226 | 2.39E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene66979 | 0.841623 | 4.25E-58 | -1.69E-46 |
| MS.gene051902 | MS.gene66979 | 0.823707 | 1.20E-53 | -1.69E-46 |
| MS.gene052296 | MS.gene66979 | 0.844664 | 6.57E-59 | -1.69E-46 |
| MS.gene052533 | MS.gene66979 | 0.826145 | 3.19E-54 | -1.69E-46 |
| MS.gene052793 | MS.gene66979 | 0.818883 | 1.55E-52 | -1.69E-46 |
| MS.gene052818 | MS.gene66979 | -0.826616 | 2.46E-54 | -1.69E-46 |
| MS.gene053045 | MS.gene66979 | 0.844736 | 6.28E-59 | -1.69E-46 |
| MS.gene053298 | MS.gene66979 | 0.83089 | 2.28E-55 | -1.69E-46 |
| MS.gene053340 | MS.gene66979 | 0.817665 | 2.93E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene66979 | 0.814893 | 1.22E-51 | -1.69E-46 |
| MS.gene054222 | MS.gene66979 | 0.856602 | 2.88E-62 | -1.69E-46 |
| MS.gene054403 | MS.gene66979 | 0.80173 | 7.84E-49 | -1.69E-46 |
| MS.gene055555 | MS.gene66979 | 0.815658 | 8.26E-52 | -1.69E-46 |
| MS.gene056677 | MS.gene66979 | 0.809341 | 1.99E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene66979 | 0.852712 | 3.86E-61 | -1.69E-46 |
| MS.gene057576 | MS.gene66979 | 0.816443 | 5.52E-52 | -1.69E-46 |
| MS.gene057584 | MS.gene66979 | 0.821368 | 4.19E-53 | -1.69E-46 |
| MS.gene057588 | MS.gene66979 | 0.804183 | 2.44E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene66979 | 0.802371 | 5.79E-49 | -1.69E-46 |
| MS.gene057893 | MS.gene66979 | -0.80186 | 7.38E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene66979 | 0.810243 | 1.27E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene66979 | 0.801907 | 7.22E-49 | -1.69E-46 |
| MS.gene058074 | MS.gene66979 | 0.827215 | 1.77E-54 | -1.69E-46 |
| MS.gene058159 | MS.gene66979 | 0.806746 | 7.08E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene66979 | 0.802883 | 4.54E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene66979 | 0.830336 | 3.11E-55 | -1.69E-46 |
| MS.gene05985 | MS.gene66979 | -0.836308 | 1.01E-56 | -1.69E-46 |
| MS.gene06018 | MS.gene66979 | 0.834115 | 3.61E-56 | -1.69E-46 |
| MS.gene061100 | MS.gene66979 | 0.822442 | 2.36E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66979.t1 | MTR_6g029390 | 97.778 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 9.28e-62 | 182 |
| MS.gene66979.t1 | MTR_5g098200 | 83.333 | 84 | 14 | 0 | 1 | 84 | 1 | 84 | 4.45e-48 | 147 |
| MS.gene66979.t1 | MTR_7g085610 | 61.798 | 89 | 31 | 2 | 1 | 88 | 1 | 87 | 8.80e-35 | 114 |
| MS.gene66979.t1 | MTR_5g098280 | 47.368 | 76 | 37 | 1 | 1 | 76 | 1 | 73 | 1.80e-20 | 77.8 |
| MS.gene66979.t1 | MTR_3g466420 | 46.341 | 82 | 38 | 1 | 1 | 76 | 1 | 82 | 1.46e-19 | 75.9 |
| MS.gene66979.t1 | MTR_5g075070 | 42.697 | 89 | 42 | 2 | 1 | 80 | 1 | 89 | 3.35e-19 | 75.1 |
| MS.gene66979.t1 | MTR_0398s0030 | 43.210 | 81 | 41 | 1 | 1 | 76 | 1 | 81 | 1.34e-17 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66979.t1 | AT5G18150 | 62.821 | 78 | 21 | 2 | 1 | 78 | 1 | 70 | 8.32e-31 | 103 |
| MS.gene66979.t1 | AT5G14602 | 57.143 | 84 | 32 | 2 | 1 | 84 | 1 | 80 | 4.76e-30 | 102 |
| MS.gene66979.t1 | AT5G58375 | 44.048 | 84 | 42 | 2 | 1 | 81 | 1 | 82 | 1.03e-16 | 68.6 |
Find 19 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGATTCAAAATGGATTT+TGG | 0.097169 | 6.4:-3855120 | MS.gene66979:CDS |
| AGAGAGAGATGTGTCCTTTA+AGG | 0.173917 | 6.4:-3855804 | None:intergenic |
| ATTGAAATCCTCTAAGAATT+AGG | 0.190751 | 6.4:-3855029 | None:intergenic |
| ATTGAGTCCCTAATTCTTAG+AGG | 0.300143 | 6.4:+3855021 | None:intergenic |
| AACACCAAGATGAACCTTAA+AGG | 0.300317 | 6.4:+3855790 | None:intergenic |
| GGTGTTCTTCTCTGCGGTTC+TGG | 0.325709 | 6.4:-3855773 | MS.gene66979:CDS |
| ATCTTCACCAAAGATTGATT+TGG | 0.357200 | 6.4:-3855722 | MS.gene66979:CDS |
| TTGAAATCCTCTAAGAATTA+GGG | 0.358948 | 6.4:-3855028 | None:intergenic |
| TCACAAGCCAAATCAATCTT+TGG | 0.382003 | 6.4:+3855715 | None:intergenic |
| TTCTGGCAGGTTACTTTGCA+TGG | 0.389470 | 6.4:-3855756 | MS.gene66979:CDS |
| TGGCTAGTGGAAGGTATCTA+TGG | 0.397595 | 6.4:-3855084 | MS.gene66979:CDS |
| TTCTTCTCTGCGGTTCTGGC+AGG | 0.404540 | 6.4:-3855769 | MS.gene66979:CDS |
| GTGTCCTTTAAGGTTCATCT+TGG | 0.474313 | 6.4:-3855794 | MS.gene66979:CDS |
| GGTATCTATGGAGAAATTTG+AGG | 0.538445 | 6.4:-3855072 | MS.gene66979:CDS |
| GGCTTGTGATGATTCAATTG+AGG | 0.570775 | 6.4:-3855701 | MS.gene66979:CDS |
| AGGTTACTTTGCATGGAGAA+CGG | 0.580884 | 6.4:-3855749 | MS.gene66979:CDS |
| GTATTCGTTGACATGGCTAG+TGG | 0.585469 | 6.4:-3855097 | MS.gene66979:CDS |
| CATCTTGGTGTTCTTCTCTG+CGG | 0.597461 | 6.4:-3855779 | MS.gene66979:CDS |
| TCGTTGACATGGCTAGTGGA+AGG | 0.632760 | 6.4:-3855093 | MS.gene66979:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTATATAATTGATCT+TGG | - | chr6.4:3855215-3855234 | MS.gene66979:intron | 10.0% |
| !!! | TTATTTTTAATTTTTGTGTT+TGG | - | chr6.4:3855639-3855658 | MS.gene66979:intron | 10.0% |
| !! | AAAATTAAAATTCCAAGATT+TGG | + | chr6.4:3855298-3855317 | None:intergenic | 15.0% |
| !! | AATTAATAACTAATGTTGAT+TGG | - | chr6.4:3855360-3855379 | MS.gene66979:intron | 15.0% |
| !!! | ACATAACTTTAAAAAGATAA+AGG | + | chr6.4:3855243-3855262 | None:intergenic | 15.0% |
| !!! | ATTAATAACTAATGTTGATT+GGG | - | chr6.4:3855361-3855380 | MS.gene66979:intron | 15.0% |
| !!! | ATGATATGCTAATTGATTTT+GGG | - | chr6.4:3855486-3855505 | MS.gene66979:intron | 20.0% |
| !!! | ATGTTTTATTGTTAAGGTTT+GGG | - | chr6.4:3855394-3855413 | MS.gene66979:intron | 20.0% |
| !!! | TAAATCTTGGAATTTTCTTT+GGG | - | chr6.4:3855579-3855598 | MS.gene66979:intron | 20.0% |
| !!! | TAGATGATGTTTTATTGTTA+AGG | - | chr6.4:3855388-3855407 | MS.gene66979:intron | 20.0% |
| !!! | TGTTTTGGTTTTTTAATCAT+GGG | - | chr6.4:3855443-3855462 | MS.gene66979:intron | 20.0% |
| !!! | TTAAATCTTGGAATTTTCTT+TGG | - | chr6.4:3855578-3855597 | MS.gene66979:intron | 20.0% |
| ! | AGAAAAATCATCTTTCAAGA+AGG | - | chr6.4:3855149-3855168 | MS.gene66979:intron | 25.0% |
| ! | ATTCACCAAAATATGTTGAT+TGG | - | chr6.4:3855533-3855552 | MS.gene66979:intron | 25.0% |
| ! | TTAATCATGGGAATAATGAT+TGG | - | chr6.4:3855455-3855474 | MS.gene66979:intron | 25.0% |
| !! | AACTCATCAAAATGTTGATT+GGG | - | chr6.4:3855678-3855697 | MS.gene66979:CDS | 25.0% |
| !! | TAAACCCAATCAACATATTT+TGG | + | chr6.4:3855541-3855560 | None:intergenic | 25.0% |
| !! | TTCACCAAAATATGTTGATT+GGG | - | chr6.4:3855534-3855553 | MS.gene66979:intron | 25.0% |
| !!! | AGATGATTCAAAATGGATTT+TGG | - | chr6.4:3855706-3855725 | MS.gene66979:CDS | 25.0% |
| !!! | GATGATATGCTAATTGATTT+TGG | - | chr6.4:3855485-3855504 | MS.gene66979:intron | 25.0% |
| !!! | GATGATTCAAAATGGATTTT+GGG | - | chr6.4:3855707-3855726 | MS.gene66979:CDS | 25.0% |
| !!! | GATGTTTTATTGTTAAGGTT+TGG | - | chr6.4:3855393-3855412 | MS.gene66979:intron | 25.0% |
| !!! | GGAAGATTTTGATTTCAAAA+AGG | - | chr6.4:3855170-3855189 | MS.gene66979:intron | 25.0% |
| !!! | GTGTTTTGGTTTTTTAATCA+TGG | - | chr6.4:3855442-3855461 | MS.gene66979:intron | 25.0% |
| ATCTTCACCAAAGATTGATT+TGG | - | chr6.4:3855104-3855123 | MS.gene66979:CDS | 30.0% | |
| GAACTCATCAAAATGTTGAT+TGG | - | chr6.4:3855677-3855696 | MS.gene66979:CDS | 30.0% | |
| ! | CTTTGTGTTTGATTGTGTTT+TGG | - | chr6.4:3855428-3855447 | MS.gene66979:intron | 30.0% |
| ! | GAGGTTTTCATGTTAAATCT+TGG | - | chr6.4:3855566-3855585 | MS.gene66979:intron | 30.0% |
| ! | GGTTTTCAGATGATTCAAAA+TGG | - | chr6.4:3855699-3855718 | MS.gene66979:CDS | 30.0% |
| AACACCAAGATGAACCTTAA+AGG | + | chr6.4:3855039-3855058 | None:intergenic | 35.0% | |
| GGTATCTATGGAGAAATTTG+AGG | - | chr6.4:3855754-3855773 | MS.gene66979:CDS | 35.0% | |
| GTTGATTGGGTTTATAGATG+AGG | - | chr6.4:3855547-3855566 | MS.gene66979:intron | 35.0% | |
| TAGTCTACGAATCCAAATCT+TGG | - | chr6.4:3855283-3855302 | MS.gene66979:intron | 35.0% | |
| TCACAAGCCAAATCAATCTT+TGG | + | chr6.4:3855114-3855133 | None:intergenic | 35.0% | |
| !! | ATTTTGGGTATTCGTTGACA+TGG | - | chr6.4:3855722-3855741 | MS.gene66979:CDS | 35.0% |
| !! | CTTGGAATTTTCTTTGGGTT+TGG | - | chr6.4:3855584-3855603 | MS.gene66979:intron | 35.0% |
| GGCTTGTGATGATTCAATTG+AGG | - | chr6.4:3855125-3855144 | MS.gene66979:intron | 40.0% | |
| ! | AGGTTACTTTGCATGGAGAA+CGG | - | chr6.4:3855077-3855096 | MS.gene66979:CDS | 40.0% |
| !! | GTGTCCTTTAAGGTTCATCT+TGG | - | chr6.4:3855032-3855051 | MS.gene66979:CDS | 40.0% |
| GTATTCGTTGACATGGCTAG+TGG | - | chr6.4:3855729-3855748 | MS.gene66979:CDS | 45.0% | |
| ! | TTCTGGCAGGTTACTTTGCA+TGG | - | chr6.4:3855070-3855089 | MS.gene66979:CDS | 45.0% |
| !! | CATCTTGGTGTTCTTCTCTG+CGG | - | chr6.4:3855047-3855066 | MS.gene66979:CDS | 45.0% |
| !! | TGGCTAGTGGAAGGTATCTA+TGG | - | chr6.4:3855742-3855761 | MS.gene66979:CDS | 45.0% |
| !!! | AAAAATAATAATTTTTTTGA+AGG | + | chr6.4:3855201-3855220 | None:intergenic | 5.0% |
| !!! | AAAATAATAATTTTTTTGAA+GGG | + | chr6.4:3855200-3855219 | None:intergenic | 5.0% |
| TCGTTGACATGGCTAGTGGA+AGG | - | chr6.4:3855733-3855752 | MS.gene66979:CDS | 50.0% | |
| GGTGTTCTTCTCTGCGGTTC+TGG | - | chr6.4:3855053-3855072 | MS.gene66979:CDS | 55.0% | |
| ! | TTCTTCTCTGCGGTTCTGGC+AGG | - | chr6.4:3855057-3855076 | MS.gene66979:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 3855030 | 3855818 | 3855030 | ID=MS.gene66979 |
| chr6.4 | mRNA | 3855030 | 3855818 | 3855030 | ID=MS.gene66979.t1;Parent=MS.gene66979 |
| chr6.4 | exon | 3855657 | 3855818 | 3855657 | ID=MS.gene66979.t1.exon1;Parent=MS.gene66979.t1 |
| chr6.4 | CDS | 3855657 | 3855818 | 3855657 | ID=cds.MS.gene66979.t1;Parent=MS.gene66979.t1 |
| chr6.4 | exon | 3855030 | 3855140 | 3855030 | ID=MS.gene66979.t1.exon2;Parent=MS.gene66979.t1 |
| chr6.4 | CDS | 3855030 | 3855140 | 3855030 | ID=cds.MS.gene66979.t1;Parent=MS.gene66979.t1 |
| Gene Sequence |
| Protein sequence |
