AlfalfaGEDB | MS.gene67072

M. sativa cultivar XinJiangDaYe / MS.gene67072

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67072.t1	XP_024629238.1	91.1	124	11	0	1	124	15	138	5.50E-53	216.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67072.t1	Q8RYD1	47.5	120	61	2	1	119	15	133	3.5e-24	112.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67072.t1	A0A072VE24	81.5	124	23	0	1	124	13	136	2.2e-51	211.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene67072.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene67072	0.809979	1.45E-50	-1.69E-46
MS.gene049435	MS.gene67072	0.818271	2.14E-52	-1.69E-46
MS.gene049501	MS.gene67072	0.81493	1.20E-51	-1.69E-46
MS.gene049850	MS.gene67072	0.807667	4.52E-50	-1.69E-46
MS.gene050226	MS.gene67072	0.811448	6.97E-51	-1.69E-46
MS.gene050227	MS.gene67072	0.806091	9.73E-50	-1.69E-46
MS.gene050228	MS.gene67072	0.814418	1.56E-51	-1.69E-46
MS.gene050442	MS.gene67072	0.839625	1.42E-57	-1.69E-46
MS.gene050780	MS.gene67072	0.801016	1.10E-48	-1.69E-46
MS.gene050807	MS.gene67072	0.804361	2.24E-49	-1.69E-46
MS.gene051247	MS.gene67072	0.822169	2.73E-53	-1.69E-46
MS.gene051744	MS.gene67072	0.807513	4.87E-50	-1.69E-46
MS.gene052020	MS.gene67072	0.832612	8.56E-56	-1.69E-46
MS.gene052323	MS.gene67072	0.813712	2.23E-51	-1.69E-46
MS.gene052611	MS.gene67072	0.806114	9.63E-50	-1.69E-46
MS.gene052665	MS.gene67072	0.806448	8.19E-50	-1.69E-46
MS.gene052896	MS.gene67072	0.812879	3.39E-51	-1.69E-46
MS.gene053074	MS.gene67072	0.803647	3.16E-49	-1.69E-46
MS.gene053641	MS.gene67072	0.805541	1.27E-49	-1.69E-46
MS.gene05415	MS.gene67072	0.804852	1.77E-49	-1.69E-46
MS.gene054236	MS.gene67072	0.806983	6.31E-50	-1.69E-46
MS.gene054467	MS.gene67072	0.845368	4.24E-59	-1.69E-46
MS.gene055356	MS.gene67072	0.808673	2.76E-50	-1.69E-46
MS.gene055357	MS.gene67072	0.827367	1.63E-54	-1.69E-46
MS.gene055358	MS.gene67072	0.800257	1.57E-48	-1.69E-46
MS.gene055359	MS.gene67072	0.807113	5.92E-50	-1.69E-46
MS.gene055818	MS.gene67072	0.824018	1.01E-53	-1.69E-46
MS.gene05604	MS.gene67072	0.820993	5.11E-53	-1.69E-46
MS.gene056173	MS.gene67072	0.816983	4.17E-52	-1.69E-46
MS.gene057869	MS.gene67072	0.84089	6.62E-58	-1.69E-46
MS.gene057915	MS.gene67072	0.825948	3.55E-54	-1.69E-46
MS.gene058168	MS.gene67072	0.82158	3.74E-53	-1.69E-46
MS.gene058636	MS.gene67072	0.815279	1.00E-51	-1.69E-46
MS.gene059006	MS.gene67072	0.800624	1.32E-48	-1.69E-46
MS.gene059124	MS.gene67072	0.806025	1.01E-49	-1.69E-46
MS.gene059128	MS.gene67072	0.809247	2.08E-50	-1.69E-46
MS.gene060137	MS.gene67072	-0.8205	6.64E-53	-1.69E-46
MS.gene060803	MS.gene67072	0.816564	5.18E-52	-1.69E-46
MS.gene060998	MS.gene67072	0.819393	1.19E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67072.t1	MTR_1g021440	87.097	124	16	0	1	124	13	136	7.12e-79	234
MS.gene67072.t1	MTR_1g021440	87.097	124	16	0	1	124	13	136	8.87e-79	234
MS.gene67072.t1	MTR_1g021440	87.097	124	16	0	1	124	13	136	3.48e-77	233
MS.gene67072.t1	MTR_1g021440	87.097	124	16	0	1	124	13	136	3.73e-77	233
MS.gene67072.t1	MTR_1g021350	91.525	118	10	0	1	118	12	129	5.19e-77	231
MS.gene67072.t1	MTR_1g021330	86.400	125	16	1	1	124	16	140	6.16e-74	224
MS.gene67072.t1	MTR_1g021400	84.800	125	18	1	1	124	20	144	7.59e-73	221
MS.gene67072.t1	MTR_1g021410	82.114	123	22	0	1	123	13	135	6.62e-70	213
MS.gene67072.t1	MTR_1g021435	82.400	125	21	1	1	124	12	136	3.40e-69	212
MS.gene67072.t1	MTR_1g021360	82.114	123	22	0	1	123	16	138	4.12e-69	215
MS.gene67072.t1	MTR_1g021360	80.645	124	23	1	1	124	174	296	1.67e-67	211
MS.gene67072.t1	MTR_1g021320	82.258	124	21	1	1	123	15	138	2.81e-67	217
MS.gene67072.t1	MTR_1g021320	72.581	124	33	1	1	124	174	296	2.22e-57	190
MS.gene67072.t1	MTR_1g021290	80.165	121	24	0	1	121	18	138	7.09e-66	203
MS.gene67072.t1	MTR_1g021290	74.194	124	31	1	1	124	147	269	4.02e-60	188
MS.gene67072.t1	MTR_1g021380	82.301	113	20	0	1	113	12	124	3.05e-65	201
MS.gene67072.t1	MTR_1g021500	58.594	128	31	3	1	124	17	126	2.90e-42	144
MS.gene67072.t1	MTR_1g021500	58.594	128	31	3	1	124	17	126	3.99e-42	145
MS.gene67072.t1	MTR_7g050710	35.965	114	72	1	11	124	14	126	1.33e-16	74.3
MS.gene67072.t1	MTR_7g050640	38.824	85	52	0	23	107	26	110	2.28e-16	69.7
MS.gene67072.t1	MTR_1g021270	37.383	107	65	2	10	116	15	119	2.05e-15	71.2
MS.gene67072.t1	MTR_7g050580	36.170	94	60	0	11	104	14	107	2.38e-15	70.5
MS.gene67072.t1	MTR_3g098790	34.021	97	63	1	9	104	11	107	3.49e-15	70.5
MS.gene67072.t1	MTR_7g050720	35.294	102	65	1	4	104	3	104	6.33e-15	69.7
MS.gene67072.t1	MTR_7g050560	34.021	97	63	1	9	104	11	107	9.02e-13	63.5
MS.gene67072.t1	MTR_5g044670	35.106	94	61	0	11	104	17	110	1.78e-12	62.8
MS.gene67072.t1	MTR_4g068320	36.047	86	55	0	15	100	11	96	3.46e-12	62.0
MS.gene67072.t1	MTR_7g050590	39.189	74	45	0	26	99	2	75	5.08e-12	61.2
MS.gene67072.t1	MTR_1g046200	30.476	105	71	1	11	113	8	112	7.61e-12	60.8
MS.gene67072.t1	MTR_8g103940	30.392	102	70	1	4	104	32	133	1.03e-11	60.8
MS.gene67072.t1	MTR_1g034240	36.782	87	55	0	14	100	11	97	1.93e-11	60.1
MS.gene67072.t1	MTR_3g005480	32.292	96	64	1	10	104	20	115	9.72e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67072.t1	AT4G33280	52.000	125	58	2	1	124	15	138	2.28e-41	138
MS.gene67072.t1	AT4G33280	52.000	125	58	2	1	124	15	138	3.03e-40	138
MS.gene67072.t1	AT4G33280	53.333	120	54	2	1	119	15	133	5.49e-40	136
MS.gene67072.t1	AT4G34400	37.383	107	62	3	4	107	8	112	2.37e-20	85.1
MS.gene67072.t1	AT4G34400	37.383	107	62	3	4	107	8	112	2.37e-20	85.1
MS.gene67072.t1	AT3G06220	33.333	105	69	1	11	115	11	114	8.92e-16	69.7
MS.gene67072.t1	AT3G06160	32.381	105	69	2	11	115	70	172	5.79e-15	70.1
MS.gene67072.t1	AT3G06160	32.381	105	69	2	11	115	26	128	4.81e-14	67.4
MS.gene67072.t1	AT3G06160	32.143	112	74	2	11	122	11	120	4.93e-14	67.0
MS.gene67072.t1	AT3G06160	32.143	112	74	2	11	122	11	120	4.93e-14	67.0
MS.gene67072.t1	AT3G06160	32.143	112	74	2	11	122	11	120	4.93e-14	67.0
MS.gene67072.t1	AT3G18990	36.559	93	58	1	8	100	5	96	6.14e-14	67.0
MS.gene67072.t1	AT3G18990	36.559	93	58	1	8	100	5	96	7.23e-14	67.0
MS.gene67072.t1	AT5G60130	37.363	91	52	2	22	107	25	115	1.04e-12	62.8
MS.gene67072.t1	AT5G60140	32.110	109	70	2	2	107	7	114	1.17e-12	63.2
MS.gene67072.t1	AT5G60130	38.202	89	50	2	24	107	1	89	1.17e-12	63.2
MS.gene67072.t1	AT5G60130	37.363	91	52	2	22	107	25	115	1.45e-12	62.8
MS.gene67072.t1	AT5G60130	37.363	91	52	2	22	107	25	115	1.48e-12	63.2
MS.gene67072.t1	AT5G60130	37.363	91	52	2	22	107	22	112	1.60e-12	62.8
MS.gene67072.t1	AT5G66980	39.326	89	49	2	23	107	22	109	2.21e-12	62.4
MS.gene67072.t1	AT3G53310	35.443	79	51	0	22	100	22	100	2.49e-12	62.0
MS.gene67072.t1	AT4G01580	36.458	96	60	1	11	106	31	125	2.03e-11	58.5
MS.gene67072.t1	AT3G18960	36.458	96	60	1	11	106	31	125	5.22e-11	57.8
MS.gene67072.t1	AT3G18960	36.458	96	60	1	11	106	31	125	7.79e-11	57.4

Find 30 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGCTCCACTAGGACCTTTA+AGG	0.180866	1.2:-3205884	None:intergenic
TAACAAGTTGTTGTTGAAAA+TGG	0.201740	1.2:-3205499	None:intergenic
CCAGAAAATGTGACCCTTAA+AGG	0.236805	1.2:+3205870	MS.gene67072:CDS
AGTGGAGCTGTGTGGGATAT+TGG	0.305311	1.2:+3205897	MS.gene67072:CDS
CATGGGAGGAAGAGATTTAC+TGG	0.311171	1.2:+3205432	None:intergenic
AATGGGTGGTACTCAGAAAT+TGG	0.319733	1.2:-3205481	None:intergenic
TGAAATGGATGAACTGAAAA+TGG	0.343707	1.2:-3205457	None:intergenic
TACTCAGAAATTGGGTGAAA+TGG	0.383889	1.2:-3205472	None:intergenic
GTGGAGCTGTGTGGGATATT+GGG	0.390357	1.2:+3205898	MS.gene67072:CDS
TGTTTACTTCGTGGGCGGTT+GGG	0.420992	1.2:+3205941	MS.gene67072:CDS
CTGTTTACTTCGTGGGCGGT+TGG	0.428777	1.2:+3205940	MS.gene67072:CDS
AACAAGTTGTTGTTGAAAAT+GGG	0.464779	1.2:-3205498	None:intergenic
AGCTCCACTAGGACCTTTAA+GGG	0.472647	1.2:-3205883	None:intergenic
CAACGGTGAATCACTCTTTG+AGG	0.482140	1.2:+3206019	MS.gene67072:CDS
GATGGTACTGTTTACTTCGT+GGG	0.490529	1.2:+3205933	MS.gene67072:CDS
ATGGGTGGTACTCAGAAATT+GGG	0.499844	1.2:-3205480	None:intergenic
CACCGTTGTACTTAAAGACA+AGG	0.534444	1.2:-3206004	None:intergenic
AGATGGTACTGTTTACTTCG+TGG	0.536376	1.2:+3205932	MS.gene67072:CDS
TTCCTTGTCTTTAAGTACAA+CGG	0.557608	1.2:+3206002	MS.gene67072:CDS
AGGTCCTAGTGGAGCTGTGT+GGG	0.568073	1.2:+3205890	MS.gene67072:CDS
GGACAAGCTCATACTGAACA+AGG	0.580719	1.2:+3206104	MS.gene67072:CDS
ATATCCCACACAGCTCCACT+AGG	0.588180	1.2:-3205894	None:intergenic
AAGGTCCTAGTGGAGCTGTG+TGG	0.617527	1.2:+3205889	MS.gene67072:CDS
GTGACCCTTAAAGGTCCTAG+TGG	0.630968	1.2:+3205879	MS.gene67072:CDS
ACAAGCTCATACTGAACAAG+GGG	0.635406	1.2:+3206106	MS.gene67072:CDS
ATTGGGTTGACAACTAGAGA+TGG	0.647577	1.2:+3205915	MS.gene67072:CDS
GACAAGCTCATACTGAACAA+GGG	0.653892	1.2:+3206105	MS.gene67072:CDS
GGTACTGTTTACTTCGTGGG+CGG	0.659429	1.2:+3205936	MS.gene67072:CDS
AAGTTGTTGTTGAAAATGGG+TGG	0.700580	1.2:-3205495	None:intergenic
CAAGCTCATACTGAACAAGG+GGG	0.792313	1.2:+3206107	MS.gene67072:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTGTCTGAAAATGTTTT+AGG	-	chr1.2:3205837-3205856	None:intergenic	20.0%
!!!	GAAATTTATATTTTAACTGC+AGG	+	chr1.2:3205806-3205825	MS.gene67072:intron	20.0%
!!!	TTTGAGGTTTTAATCTTTAA+TGG	+	chr1.2:3206035-3206054	MS.gene67072:CDS	20.0%
!!!	TTTTTGTTTCATTTAGAAGA+TGG	-	chr1.2:3205554-3205573	None:intergenic	20.0%
!	TCAATACTATAGTCAAAGAA+AGG	-	chr1.2:3205582-3205601	None:intergenic	25.0%
!!	AACAAGTTGTTGTTGAAAAT+GGG	-	chr1.2:3205501-3205520	None:intergenic	25.0%
!!	ATGTATCTTTTAATCTCAGA+TGG	+	chr1.2:3205784-3205803	MS.gene67072:intron	25.0%
!!	TAACAAGTTGTTGTTGAAAA+TGG	-	chr1.2:3205502-3205521	None:intergenic	25.0%
!!!	TCTTATTTTGTTCTAGAATG+TGG	+	chr1.2:3206083-3206102	MS.gene67072:CDS	25.0%
	TGAAATGGATGAACTGAAAA+TGG	-	chr1.2:3205460-3205479	None:intergenic	30.0%
	TGATTTCAGCAATGATTTAC+AGG	-	chr1.2:3205730-3205749	None:intergenic	30.0%
	TGTTTCATTTAGAAGATGGA+AGG	-	chr1.2:3205550-3205569	None:intergenic	30.0%
	TTCCTTGTCTTTAAGTACAA+CGG	+	chr1.2:3206002-3206021	MS.gene67072:CDS	30.0%
	GAACTAACAAGTGCAGTTAA+AGG	-	chr1.2:3205654-3205673	None:intergenic	35.0%
	TACTCAGAAATTGGGTGAAA+TGG	-	chr1.2:3205475-3205494	None:intergenic	35.0%
!	AAGTTGTTGTTGAAAATGGG+TGG	-	chr1.2:3205498-3205517	None:intergenic	35.0%
	AATGGGTGGTACTCAGAAAT+TGG	-	chr1.2:3205484-3205503	None:intergenic	40.0%
	ACAAGCTCATACTGAACAAG+GGG	+	chr1.2:3206106-3206125	MS.gene67072:CDS	40.0%
	AGATGGTACTGTTTACTTCG+TGG	+	chr1.2:3205932-3205951	MS.gene67072:CDS	40.0%
	ATGGGTGGTACTCAGAAATT+GGG	-	chr1.2:3205483-3205502	None:intergenic	40.0%
	CACCGTTGTACTTAAAGACA+AGG	-	chr1.2:3206007-3206026	None:intergenic	40.0%
	CCAGAAAATGTGACCCTTAA+AGG	+	chr1.2:3205870-3205889	MS.gene67072:CDS	40.0%
	GACAAGCTCATACTGAACAA+GGG	+	chr1.2:3206105-3206124	MS.gene67072:CDS	40.0%
	GATGGTACTGTTTACTTCGT+GGG	+	chr1.2:3205933-3205952	MS.gene67072:CDS	40.0%
!!	ATTGGGTTGACAACTAGAGA+TGG	+	chr1.2:3205915-3205934	MS.gene67072:CDS	40.0%
!!	CCTTTAAGGGTCACATTTTC+TGG	-	chr1.2:3205873-3205892	None:intergenic	40.0%
	AGCTCCACTAGGACCTTTAA+GGG	-	chr1.2:3205886-3205905	None:intergenic	45.0%
	CAACGGTGAATCACTCTTTG+AGG	+	chr1.2:3206019-3206038	MS.gene67072:CDS	45.0%
	GGACAAGCTCATACTGAACA+AGG	+	chr1.2:3206104-3206123	MS.gene67072:CDS	45.0%
	AGTGGAGCTGTGTGGGATAT+TGG	+	chr1.2:3205897-3205916	MS.gene67072:CDS	50.0%
	ATATCCCACACAGCTCCACT+AGG	-	chr1.2:3205897-3205916	None:intergenic	50.0%
	CAGCTCCACTAGGACCTTTA+AGG	-	chr1.2:3205887-3205906	None:intergenic	50.0%
	GGTACTGTTTACTTCGTGGG+CGG	+	chr1.2:3205936-3205955	MS.gene67072:CDS	50.0%
	GTGACCCTTAAAGGTCCTAG+TGG	+	chr1.2:3205879-3205898	MS.gene67072:CDS	50.0%
	GTGGAGCTGTGTGGGATATT+GGG	+	chr1.2:3205898-3205917	MS.gene67072:CDS	50.0%
	TGTTTACTTCGTGGGCGGTT+GGG	+	chr1.2:3205941-3205960	MS.gene67072:CDS	50.0%
	AAGGTCCTAGTGGAGCTGTG+TGG	+	chr1.2:3205889-3205908	MS.gene67072:CDS	55.0%
	AGGTCCTAGTGGAGCTGTGT+GGG	+	chr1.2:3205890-3205909	MS.gene67072:CDS	55.0%
	CTGTTTACTTCGTGGGCGGT+TGG	+	chr1.2:3205940-3205959	MS.gene67072:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	3205446	3206128	3205446	ID=MS.gene67072
chr1.2	mRNA	3205446	3206128	3205446	ID=MS.gene67072.t1;Parent=MS.gene67072
chr1.2	exon	3205446	3205517	3205446	ID=MS.gene67072.t1.exon1;Parent=MS.gene67072.t1
chr1.2	CDS	3205446	3205517	3205446	ID=cds.MS.gene67072.t1;Parent=MS.gene67072.t1
chr1.2	exon	3205828	3206128	3205828	ID=MS.gene67072.t1.exon2;Parent=MS.gene67072.t1
chr1.2	CDS	3205828	3206128	3205828	ID=cds.MS.gene67072.t1;Parent=MS.gene67072.t1

Gene Sequence

>MS.gene67072

ATTTACTGGAACCATTTTCAGTTCATCCATTTCACCCAATTTCTGAGTACCACCCATTTTCAACAACAACTTGTTAGTACTTCTTTTTGCTCTTTTTCTTTCCTTCCATCTTCTAAATGAAACAAAAATGAAACCTTTCTTTGACTATAGTATTGAGTGCATGCACTTCATCTCATGTTCATAAATTTTTTATCTTTTTACACTTCCTTTAACTGCACTTGTTAGTTCTTTTGAACAAAGTTTTCAGCTTTTCATTTTTAGCTTTTCAGTTTTTAGCTTTTCCTGTAAATCATTGCTGAAATCATGTACTTGTGATGATAATAGCTGTTTTTCAATCAATGTATCTTTTAATCTCAGATGGAAATTTATATTTTAACTGCAGGCACTTCCTAAAACATTTTCAGACAATTTAAAAAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCTAGTGGAGCTGTGTGGGATATTGGGTTGACAACTAGAGATGGTACTGTTTACTTCGTGGGCGGTTGGGAGCAATTCATGAAAGATCACTCTTTGAAAGAGAATGATTTCCTTGTCTTTAAGTACAACGGTGAATCACTCTTTGAGGTTTTAATCTTTAATGGAGAAAGCTTATGTGAGAAAGCAGCTTCTTATTTTGTTCTAGAATGTGGACAAGCTCATACTGAACAAGGGG

Protein sequence

>MS.gene67072.t1

IYWNHFQFIHFTQFLSTTHFQQQLALPKTFSDNLKKKLPENVTLKGPSGAVWDIGLTTRDGTVYFVGGWEQFMKDHSLKENDFLVFKYNGESLFEVLIFNGESLCEKAASYFVLECGQAHTEQG