AlfalfaGEDB | MS.gene69477

M. sativa cultivar XinJiangDaYe / MS.gene69477

KEGG: K12832
GO: -
PFAM: PF07189.14

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69477.t1	XP_003628625.1	97.4	77	2	0	1	77	1	77	2.20E-34	156

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69477.t1	P58728	88.3	77	9	0	1	77	1	77	2.5e-34	147.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69477.t1	G7IW51	97.4	77	2	0	1	77	1	77	1.6e-34	156.0

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene69477	0.808271	3.36E-50	-1.69E-46
MS.gene049255	MS.gene69477	0.855454	6.24E-62	-1.69E-46
MS.gene049341	MS.gene69477	0.812399	4.32E-51	-1.69E-46
MS.gene049382	MS.gene69477	0.81134	7.35E-51	-1.69E-46
MS.gene049653	MS.gene69477	0.815981	6.99E-52	-1.69E-46
MS.gene049756	MS.gene69477	0.822761	1.99E-53	-1.69E-46
MS.gene050277	MS.gene69477	0.849561	2.99E-60	-1.69E-46
MS.gene050689	MS.gene69477	0.814146	1.79E-51	-1.69E-46
MS.gene050807	MS.gene69477	0.834727	2.54E-56	-1.69E-46
MS.gene050815	MS.gene69477	0.805096	1.57E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69477.t1	MTR_3g019310	97.403	77	2	1	77	1	77	3.33e-50	159
MS.gene69477.t1	MTR_3g019300	97.403	77	2	1	77	1	77	3.33e-50	159
MS.gene69477.t1	MTR_8g062750	97.403	77	2	1	77	1	77	4.83e-50	159

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69477.t1	AT4G14342	88.312	77	9	1	77	1	77	2.25e-46	149
MS.gene69477.t1	AT4G14342	87.013	77	10	1	77	1	77	6.18e-46	149
MS.gene69477.t1	AT3G23325	87.013	77	10	1	77	1	77	1.20e-45	147

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Find 47 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGGAAAGCGGTGAAGTGG+AGG	0.509855	8.1:+40985464	MS.gene69477:CDS
ACAAAGCCTAAGAATGCTCC+TGG	0.510487	8.1:+40985408	MS.gene69477:CDS
ACACAATCACTCCTATTTCC+AGG	0.369049	8.1:-40985426	None:intergenic
ACATCATGTTCAATGGGATT+AGG	0.403952	8.1:-40985630	None:intergenic
ACATGATGTTATAAAGGATG+AGG	0.598600	8.1:+40985644	MS.gene69477:CDS
AGAACGATGAAGAACTTTCA+AGG	0.532477	8.1:-40984749	None:intergenic
AGCTTGAAGATGCTCAAGTT+GGG	0.482407	8.1:-40983744	None:intergenic
AGGCTTTGTTTCTATAGCGA+AGG	0.562793	8.1:-40985394	None:intergenic
AGGGAAAGCGGTGAAGTGGA+GGG	0.601469	8.1:+40985465	MS.gene69477:CDS
AGTAAGAAAGTAAAGGGTAA+TGG	0.502423	8.1:-40983853	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACATATCTATGAATACGC+AGG	+	chr8.1:40984279-40984298	MS.gene69477:intron	30.0%
AAAGTAGCACTAGACAAAAA+TGG	-	chr8.1:40985203-40985222	None:intergenic	30.0%
AAATCATAGGAAACGATTAG+AGG	+	chr8.1:40984669-40984688	MS.gene69477:intron	30.0%
AAGCGATTTAAGAGAGAATT+GGG	+	chr8.1:40984701-40984720	MS.gene69477:intron	30.0%
AATAAGGATGAAACAGATGA+GGG	-	chr8.1:40985264-40985283	None:intergenic	30.0%
AATCATAGGAAACGATTAGA+GGG	+	chr8.1:40984670-40984689	MS.gene69477:intron	30.0%
ACACCTCAAAACAAAAAACA+TGG	-	chr8.1:40983946-40983965	None:intergenic	30.0%
ACATGATGTTATAAAGGATG+AGG	+	chr8.1:40985644-40985663	MS.gene69477:CDS	30.0%
AGTAAGAAAGTAAAGGGTAA+TGG	-	chr8.1:40983856-40983875	None:intergenic	30.0%
ATCCTTCTTAAACATGTTCA+TGG	-	chr8.1:40985553-40985572	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	40983618	40985707	40983618	ID=MS.gene69477
chr8.1	mRNA	40983618	40985707	40983618	ID=MS.gene69477.t1;Parent=MS.gene69477
chr8.1	exon	40983618	40983623	40983618	ID=MS.gene69477.t1.exon1;Parent=MS.gene69477.t1
chr8.1	CDS	40983618	40983623	40983618	ID=cds.MS.gene69477.t1;Parent=MS.gene69477.t1
chr8.1	exon	40983719	40983928	40983719	ID=MS.gene69477.t1.exon2;Parent=MS.gene69477.t1
chr8.1	CDS	40983719	40983928	40983719	ID=cds.MS.gene69477.t1;Parent=MS.gene69477.t1
chr8.1	exon	40984563	40984634	40984563	ID=MS.gene69477.t1.exon3;Parent=MS.gene69477.t1
chr8.1	CDS	40984563	40984634	40984563	ID=cds.MS.gene69477.t1;Parent=MS.gene69477.t1
chr8.1	exon	40984732	40984778	40984732	ID=MS.gene69477.t1.exon4;Parent=MS.gene69477.t1
chr8.1	CDS	40984732	40984778	40984732	ID=cds.MS.gene69477.t1;Parent=MS.gene69477.t1
chr8.1	exon	40984983	40985012	40984983	ID=MS.gene69477.t1.exon5;Parent=MS.gene69477.t1
chr8.1	CDS	40984983	40985012	40984983	ID=cds.MS.gene69477.t1;Parent=MS.gene69477.t1
chr8.1	exon	40985353	40985707	40985353	ID=MS.gene69477.t1.exon6;Parent=MS.gene69477.t1
chr8.1	CDS	40985353	40985707	40985353	ID=cds.MS.gene69477.t1;Parent=MS.gene69477.t1

Gene Sequence

>MS.gene69477

ATGCAGGTAACAGTTTTCTTCGATTATCAATTTCTTTTATTATTCGCTTATCAATTTAACGAAATCATGAAACTACGGTGTTGCGTTGCGTTGCGTTGCAGGCGAGTGATAGGTTTAACATAAATTCCCAACTTGAGCATCTTCAAGCTAAATACGTTGGAACTGGACATGCTGATATCAACAGATTTGAATGGGCTGTGAATATTCAACGTGATAGCTATGCTTCATACATTGGCCATTACCCTTTACTTTCTTACTTTTCTATCGCTCAAAACGAATCTATTGGTAGACAGCGTTATACTTTTATGCAGGTATATTACTCATTCCATGTTTTTTGTTTTGAGGTGTAAACAAACAAACAAACAAAATTACTTGTTAATTGGAATTGCTTTCTTACTGTATGTGTGTTTTTTATACTGTTAAATTGGAAGGGTTGGACCATCAGTGTGCAACCCCGGTCAATTTAGAAACAAATCCTGTTGTTAACCTACTATATAGCCGGTCAATTAGTGGTGTACTAGTATCGTACATGTCAAATTTTGTGCCTTTTAGATATTTCACTTTGAGGAAATAGAATAATGATGTTGAGTGGATAGTATTCTCTCTCATGGAGTTTAGACAAGATTGAAGAAGTGAAGCTCATGTCAAGACATTGATGCTGAAACATATCTATGAATACGCAGGATAATTTTGTAAATAAAGAATTTTCCTTAGTGAGATAAGTAACTTGGTTAGGTTAAGATTGCAAAGTTCGACATGATATTTAGCAATAAAAATTTAAAATAGATTCCCACACACGTTGTGGTAGGTTGATATGTTTATTTTGATTACATGCTCAATACCTGTGTTGTCAGTAGTACTGCTATGGCAGGCAATAGCGAAATAGCGGCGAGGACTAGGGGTCTGCTGTGATGCTAAAGCCTGTTGCAGTGCATTTTCTTGTAGTGCAATTTGTTGCCGCAAATAGGTGACAAGGAGAGTATTAGTACTCAAACTTTGGTTTGTTATTTGGAAATGGTACGCTTAGCTAGAGAGGCAAACCGTGATTTAAAAATCATAGGAAACGATTAGAGGGAAATCTTCAAGCGATTTAAGAGAGAATTGGGGCTTTTAGGTTAGGAAGTTTGCTGACCTTGAAAGTTCTTCATCGTTCTTTCCTTTGTAATCGAGTCATATCCCTATTTATTTCTAATAAAATACCAGATTCATATCTTCAAAAAATAAAAAACAGATTTGTACCTCAGAAAGATGTATATTTGAGTTTAAGATGGGAACAGTGGGTCATGGAATAATAACCTCAGAAAGAGATTCCCATCTTCTGATTTCTGCACGCGGGTCAAAAGAATACCCATTTTCTGCTCGCAGGTCAGAAGAATACCCGTTTTCTGCTCGCAGGTTAGAAGAAGACATGTTTCTCCATGCGGGTCATAAAGAAAACCCATTTTAAAAATAGGATTATTTGGTTAAAAAATCGTGCTTATTAGTTATTATGATTTAATTATTTTAAAAACAGGGCAACCTTTAATTATTAATAATTTTGTTTAGAAACTGTCTTCGTGACCCTCTTTATCCCCTGCTGCGACCATTTTTGTCTAGTGCTACTTTTCCTTTCCATCCTTTCTTGTCCATCTCTGTCCAGTTTTCCCTCATCTGTTTCATCCTTATTCTCCCTCACGACGAATTCGTCCTGTTCTTCTCTGTTTTCATTGTTAACCAAATTGCTTCTACTTCTCAGATTGCTACTAGTGAGGGATTTGCTGCTACTCACTCTCCTGTCCTTCGCTATAGAAACAAAGCCTAAGAATGCTCCTGGAAATAGGAGTGATTGTGTAGAACTTACTGAAAAAAGGGAAAGCGGTGAAGTGGAGGGTGTTGTTATCATTGACAGCTCCAAAAATATTTTTAAGAGAAGATTTGGCTCACAGCCCACTACCATGAACATGTTTAAGAAGGATATAAATTTTGATGATGTCTCGTCTAATAATGAATGGGTTGTGGCGGAAAATTATACTCCTAATCCCATTGAACATGATGTTATAAAGGATGAGGGTGGTCATGATATGCCACCTCTAGAAAAGATTGACGATTAG

Protein sequence

>MS.gene69477.t1

MQASDRFNINSQLEHLQAKYVGTGHADINRFEWAVNIQRDSYASYIGHYPLLSYFSIAQNESIGRQRYTFMQCNLLPQIGDKESISTQTLVCYLEMVRKFADLESSSSFFPLSEEYPFSARRLLLVRDLLLLTLLSFAIETKPKNAPGNRSDCVELTEKRESGEVEGVVIIDSSKNIFKRRFGSQPTTMNMFKKDINFDDVSSNNEWVVAENYTPNPIEHDVIKDEGGHDMPPLEKIDD