AlfalfaGEDB | MS.gene69854

M. sativa cultivar XinJiangDaYe / MS.gene69854

KEGG: K07904
GO: GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00071.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69854.t1	XP_003596706.1	100	97	0	0	1	97	122	218	2.10E-44	188

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69854.t1	Q40194	92.7	96	7	0	1	96	122	217	4.5e-43	174.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69854.t1	G7IM71	100.0	97	0	0	1	97	122	218	1.5e-44	188.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene69854	0.803869	2.84E-49	-1.69E-46
MS.gene049653	MS.gene69854	0.819577	1.08E-52	-1.69E-46
MS.gene049680	MS.gene69854	0.824272	8.83E-54	-1.69E-46
MS.gene050752	MS.gene69854	0.846127	2.64E-59	-1.69E-46
MS.gene050945	MS.gene69854	0.805332	1.40E-49	-1.69E-46
MS.gene050955	MS.gene69854	0.856767	2.57E-62	-1.69E-46
MS.gene051003	MS.gene69854	0.812592	3.92E-51	-1.69E-46
MS.gene051597	MS.gene69854	0.809807	1.58E-50	-1.69E-46
MS.gene051975	MS.gene69854	0.82938	5.32E-55	-1.69E-46
MS.gene052947	MS.gene69854	0.809708	1.66E-50	-1.69E-46
MS.gene053006	MS.gene69854	0.8349	2.29E-56	-1.69E-46
MS.gene053058	MS.gene69854	0.812664	3.78E-51	-1.69E-46
MS.gene053931	MS.gene69854	0.810169	1.32E-50	-1.69E-46
MS.gene053963	MS.gene69854	0.811141	8.12E-51	-1.69E-46
MS.gene054403	MS.gene69854	0.852214	5.35E-61	-1.69E-46
MS.gene05469	MS.gene69854	0.821281	4.39E-53	-1.69E-46
MS.gene05517	MS.gene69854	0.825249	5.20E-54	-1.69E-46
MS.gene055555	MS.gene69854	0.824664	7.14E-54	-1.69E-46
MS.gene055841	MS.gene69854	0.821839	3.26E-53	-1.69E-46
MS.gene056986	MS.gene69854	0.837259	5.78E-57	-1.69E-46
MS.gene057079	MS.gene69854	0.80372	3.05E-49	-1.69E-46
MS.gene057102	MS.gene69854	0.805	1.65E-49	-1.69E-46
MS.gene057208	MS.gene69854	0.826352	2.84E-54	-1.69E-46
MS.gene057705	MS.gene69854	0.826089	3.28E-54	-1.69E-46
MS.gene05781	MS.gene69854	0.811921	5.50E-51	-1.69E-46
MS.gene057999	MS.gene69854	0.80965	1.70E-50	-1.69E-46
MS.gene059146	MS.gene69854	0.815899	7.30E-52	-1.69E-46
MS.gene059830	MS.gene69854	0.812429	4.26E-51	-1.69E-46
MS.gene05985	MS.gene69854	-0.8273	1.69E-54	-1.69E-46
MS.gene060365	MS.gene69854	0.801647	8.16E-49	-1.69E-46
MS.gene060935	MS.gene69854	0.806884	6.62E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69854.t1	MTR_2g083860	100.000	97	0	0	1	97	122	218	1.53e-66	199
MS.gene69854.t1	MTR_2g075950	76.042	96	22	1	1	96	122	216	4.91e-48	152
MS.gene69854.t1	MTR_4g088090	64.286	98	30	3	1	96	122	216	1.49e-37	125
MS.gene69854.t1	MTR_2g014990	63.265	98	31	3	1	96	122	216	3.08e-37	124
MS.gene69854.t1	MTR_4g079350	60.204	98	34	3	1	96	122	216	9.74e-37	123
MS.gene69854.t1	MTR_2g090865	60.825	97	34	3	1	96	120	213	2.51e-34	117
MS.gene69854.t1	MTR_4g064897	57.292	96	39	2	1	96	121	214	1.19e-30	108
MS.gene69854.t1	MTR_3g069510	55.670	97	42	1	1	97	121	216	3.46e-30	106
MS.gene69854.t1	MTR_1g052475	51.515	99	44	1	1	95	124	222	1.66e-27	100
MS.gene69854.t1	MTR_2g005510	48.454	97	50	0	1	97	124	220	2.51e-25	94.4
MS.gene69854.t1	MTR_0262s0050	50.000	96	47	1	1	95	124	219	6.51e-24	90.9
MS.gene69854.t1	MTR_3g086980	51.546	97	45	2	1	95	125	221	1.36e-23	90.1
MS.gene69854.t1	MTR_8g089985	55.556	81	35	1	1	80	126	206	1.16e-22	87.8
MS.gene69854.t1	MTR_6g079550	42.708	96	54	1	1	96	121	215	4.85e-21	83.2
MS.gene69854.t1	MTR_7g405790	45.361	97	52	1	1	96	124	220	9.92e-18	74.7
MS.gene69854.t1	MTR_8g027220	56.923	65	28	0	1	65	135	199	1.35e-17	74.7
MS.gene69854.t1	MTR_1g078200	39.604	101	56	2	1	96	123	223	7.44e-17	72.4
MS.gene69854.t1	MTR_7g051940	46.053	76	41	0	1	76	122	197	4.55e-16	70.1
MS.gene69854.t1	MTR_8g036220	40.777	103	54	2	1	96	125	227	6.97e-16	70.1
MS.gene69854.t1	MTR_2g027660	39.048	105	47	3	1	96	115	211	1.03e-12	61.2
MS.gene69854.t1	MTR_4g099470	35.417	96	51	2	1	96	93	177	5.01e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69854.t1	AT1G06400	75.258	97	22	1	1	97	122	216	4.29e-48	152
MS.gene69854.t1	AT1G16920	75.258	97	22	2	1	97	122	216	1.28e-45	146
MS.gene69854.t1	AT4G18800	73.196	97	22	2	1	97	122	214	2.68e-43	140
MS.gene69854.t1	AT5G45750	71.134	97	26	1	1	97	122	216	2.93e-43	140
MS.gene69854.t1	AT5G60860	64.646	99	30	3	1	97	122	217	2.14e-39	130
MS.gene69854.t1	AT3G15060	65.306	98	29	3	1	96	122	216	1.65e-38	128
MS.gene69854.t1	AT1G28550	63.265	98	32	3	1	96	122	217	6.58e-38	126
MS.gene69854.t1	AT2G33870	61.224	98	34	3	1	96	122	217	3.60e-36	122
MS.gene69854.t1	AT4G18430	59.184	98	35	3	1	96	122	216	8.92e-36	121
MS.gene69854.t1	AT1G07410	56.250	96	39	2	1	96	121	213	3.24e-30	107
MS.gene69854.t1	AT1G09630	51.546	97	46	1	1	97	121	216	2.52e-28	102
MS.gene69854.t1	AT3G12160	55.102	98	39	2	1	95	124	219	6.90e-28	101
MS.gene69854.t1	AT5G47960	57.143	98	38	2	1	95	124	220	7.60e-27	98.6
MS.gene69854.t1	AT5G59150	53.608	97	43	2	1	96	70	165	2.03e-26	95.9
MS.gene69854.t1	AT5G59150	53.608	97	43	2	1	96	121	216	9.89e-26	95.5
MS.gene69854.t1	AT3G46830	53.061	98	44	2	1	97	121	217	1.84e-25	94.7
MS.gene69854.t1	AT3G46830	53.061	98	44	2	1	97	121	217	1.84e-25	94.7
MS.gene69854.t1	AT4G39990	47.619	105	38	3	1	96	126	222	3.57e-23	89.0
MS.gene69854.t1	AT5G65270	45.794	107	40	2	1	96	126	225	4.91e-22	86.3
MS.gene69854.t1	AT2G43130	45.833	96	49	1	1	96	121	213	3.29e-19	78.6
MS.gene69854.t1	AT5G46025	63.793	58	21	0	1	58	54	111	1.08e-18	74.7
MS.gene69854.t1	AT1G05810	44.444	99	49	2	1	96	121	216	3.10e-18	75.9
MS.gene69854.t1	AT3G07410	43.750	96	54	0	1	96	121	216	3.18e-18	75.9
MS.gene69854.t1	AT5G47520	40.566	106	45	2	1	96	123	220	3.83e-18	75.9
MS.gene69854.t1	AT1G05810	44.444	99	49	2	1	96	167	262	5.76e-18	76.3
MS.gene69854.t1	AT2G31680	43.434	99	51	3	1	96	121	217	2.37e-16	71.2
MS.gene69854.t1	AT1G18200	41.758	91	46	2	1	84	122	212	1.20e-14	66.6
MS.gene69854.t1	AT4G17160	44.792	96	46	3	1	95	115	204	6.07e-14	64.7
MS.gene69854.t1	AT1G73640	50.000	60	30	0	1	60	122	181	8.30e-14	64.7
MS.gene69854.t1	AT4G17170	39.423	104	46	3	1	95	115	210	1.64e-13	63.5
MS.gene69854.t1	AT1G01200	43.878	98	44	4	1	87	137	234	2.24e-12	60.8
MS.gene69854.t1	AT1G02130	38.384	99	45	3	1	96	117	202	2.33e-12	60.5
MS.gene69854.t1	AT4G35860	36.082	97	59	2	1	95	69	164	1.08e-11	57.8
MS.gene69854.t1	AT4G35860	36.082	97	59	2	1	95	115	210	3.05e-11	57.4
MS.gene69854.t1	AT5G47200	36.458	96	50	2	1	96	117	201	7.54e-11	56.2
MS.gene69854.t1	AT4G08190	63.043	46	14	1	1	46	85	127	8.45e-11	54.7

Find 20 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATATTGTGGTCATGCTTAT+TGG	0.176164	2.1:-17634220	None:intergenic
CTTCATGGAGACTTCTGCTT+TGG	0.384252	2.1:-17634119	MS.gene69854:CDS
GAGGGAGTCTCTCTACTTCA+TGG	0.418666	2.1:-17634134	MS.gene69854:CDS
AAACGTGAAAGAGGATTCTT+CGG	0.420021	2.1:-17633969	MS.gene69854:CDS
AAGACTTTCCATCTTCTGTT+GGG	0.447210	2.1:+17634164	None:intergenic
AAAGACTTTCCATCTTCTGT+TGG	0.448264	2.1:+17634163	None:intergenic
ATTTGGATGCTGCTCGAACT+AGG	0.492151	2.1:-17633936	None:intergenic
AGCAAGAAAGCAGTTGAAGC+AGG	0.498565	2.1:-17634037	MS.gene69854:CDS
GTTGCTGTCCCAACAGAAGA+TGG	0.535125	2.1:-17634172	MS.gene69854:CDS
GCAGTTGAAGCAGGTGAAGG+TGG	0.535787	2.1:-17634028	MS.gene69854:CDS
ACAGACAATAAACGTGAAAG+AGG	0.555146	2.1:-17633978	MS.gene69854:CDS
TTATTGTCTGTCCTACAGAC+GGG	0.589827	2.1:+17633990	None:intergenic
TTTATTGTCTGTCCTACAGA+CGG	0.598655	2.1:+17633989	None:intergenic
CTTCTGTTGGGACAGCAACA+AGG	0.604051	2.1:+17634176	None:intergenic
AAAGCAGTTGAAGCAGGTGA+AGG	0.608647	2.1:-17634031	MS.gene69854:CDS
TTGGGACAGCAACAAGGTGT+CGG	0.610129	2.1:+17634182	None:intergenic
TCCTCGTCAGTCCCGTCTGT+AGG	0.610460	2.1:-17634001	MS.gene69854:CDS
CTGCTTTCTTGCTCACTATG+CGG	0.632736	2.1:+17634047	None:intergenic
TCCTACAGACGGGACTGACG+AGG	0.654473	2.1:+17634000	None:intergenic
TGTAGAAAATGCATTCACTG+AGG	0.755384	2.1:-17634086	MS.gene69854:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGTGAATGCATTTTCTACAT+TGG	+	chr2.1:17634058-17634077	None:intergenic	30.0%
	AAACGTGAAAGAGGATTCTT+CGG	-	chr2.1:17634176-17634195	MS.gene69854:CDS	35.0%
	AAAGACTTTCCATCTTCTGT+TGG	+	chr2.1:17633985-17634004	None:intergenic	35.0%
	AAGACTTTCCATCTTCTGTT+GGG	+	chr2.1:17633984-17634003	None:intergenic	35.0%
	ACAGACAATAAACGTGAAAG+AGG	-	chr2.1:17634167-17634186	MS.gene69854:CDS	35.0%
	TGTAGAAAATGCATTCACTG+AGG	-	chr2.1:17634059-17634078	MS.gene69854:CDS	35.0%
	TTTATTGTCTGTCCTACAGA+CGG	+	chr2.1:17634159-17634178	None:intergenic	35.0%
!!!	TCTTCGGTTTTGAAGAGATT+TGG	-	chr2.1:17634192-17634211	MS.gene69854:CDS	35.0%
	TTATTGTCTGTCCTACAGAC+GGG	+	chr2.1:17634158-17634177	None:intergenic	40.0%
!	ATGGAAAGTCTTTTGCAGAG+AGG	-	chr2.1:17633992-17634011	MS.gene69854:CDS	40.0%
!	TGGAAAGTCTTTTGCAGAGA+GGG	-	chr2.1:17633993-17634012	MS.gene69854:CDS	40.0%
	AAAGCAGTTGAAGCAGGTGA+AGG	-	chr2.1:17634114-17634133	MS.gene69854:CDS	45.0%
	AGCAAGAAAGCAGTTGAAGC+AGG	-	chr2.1:17634108-17634127	MS.gene69854:CDS	45.0%
	CTGCTTTCTTGCTCACTATG+CGG	+	chr2.1:17634101-17634120	None:intergenic	45.0%
	CTTCATGGAGACTTCTGCTT+TGG	-	chr2.1:17634026-17634045	MS.gene69854:CDS	45.0%
	CTTCTGTTGGGACAGCAACA+AGG	+	chr2.1:17633972-17633991	None:intergenic	50.0%
	GAGGGAGTCTCTCTACTTCA+TGG	-	chr2.1:17634011-17634030	MS.gene69854:CDS	50.0%
	GTTGCTGTCCCAACAGAAGA+TGG	-	chr2.1:17633973-17633992	MS.gene69854:CDS	50.0%
	TTGGGACAGCAACAAGGTGT+CGG	+	chr2.1:17633966-17633985	None:intergenic	50.0%
	GCAGTTGAAGCAGGTGAAGG+TGG	-	chr2.1:17634117-17634136	MS.gene69854:CDS	55.0%
	TCCTACAGACGGGACTGACG+AGG	+	chr2.1:17634148-17634167	None:intergenic	60.0%
	TCCTCGTCAGTCCCGTCTGT+AGG	-	chr2.1:17634144-17634163	MS.gene69854:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	17633937	17634230	17633937	ID=MS.gene69854
chr2.1	mRNA	17633937	17634230	17633937	ID=MS.gene69854.t1;Parent=MS.gene69854
chr2.1	exon	17633937	17634230	17633937	ID=MS.gene69854.t1.exon1;Parent=MS.gene69854.t1
chr2.1	CDS	17633937	17634230	17633937	ID=cds.MS.gene69854.t1;Parent=MS.gene69854.t1

Gene Sequence	>MS.gene69854 ATGCTTATTGGAAATAAGTCTGATCTCCGACACCTTGTTGCTGTCCCAACAGAAGATGGAAAGTCTTTTGCAGAGAGGGAGTCTCTCTACTTCATGGAGACTTCTGCTTTGGAAGCAACCAATGTAGAAAATGCATTCACTGAGGTTCTTTCTCAGATATACCGCATAGTGAGCAAGAAAGCAGTTGAAGCAGGTGAAGGTGGCAGTTCCTCGTCAGTCCCGTCTGTAGGACAGACAATAAACGTGAAAGAGGATTCTTCGGTTTTGAAGAGATTTGGATGCTGCTCGAACTAG
Protein sequence	>MS.gene69854.t1 MLIGNKSDLRHLVAVPTEDGKSFAERESLYFMETSALEATNVENAFTEVLSQIYRIVSKKAVEAGEGGSSSSVPSVGQTINVKEDSSVLKRFGCCSN