Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71718.t1 | AES60682.1 | 86 | 236 | 14 | 1 | 1 | 236 | 139 | 355 | 1.10E-112 | 416.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71718.t1 | Q9FKK6 | 49.6 | 234 | 114 | 2 | 1 | 232 | 148 | 379 | 4.0e-60 | 233.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71718.t1 | G7IA65 | 86.0 | 236 | 14 | 1 | 1 | 236 | 139 | 355 | 7.6e-113 | 416.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene71718 | 0.835737 | 1.41E-56 | -1.69E-46 |
| MS.gene049159 | MS.gene71718 | 0.807554 | 4.78E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene71718 | 0.810785 | 9.70E-51 | -1.69E-46 |
| MS.gene049343 | MS.gene71718 | 0.829632 | 4.62E-55 | -1.69E-46 |
| MS.gene049672 | MS.gene71718 | 0.811964 | 5.38E-51 | -1.69E-46 |
| MS.gene049711 | MS.gene71718 | 0.829804 | 4.19E-55 | -1.69E-46 |
| MS.gene049850 | MS.gene71718 | 0.842592 | 2.35E-58 | -1.69E-46 |
| MS.gene050156 | MS.gene71718 | 0.809477 | 1.86E-50 | -1.69E-46 |
| MS.gene051181 | MS.gene71718 | 0.806724 | 7.16E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene71718 | 0.81703 | 4.07E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene71718 | 0.830093 | 3.56E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene71718 | 0.83445 | 2.98E-56 | -1.69E-46 |
| MS.gene052516 | MS.gene71718 | 0.833368 | 5.55E-56 | -1.69E-46 |
| MS.gene052519 | MS.gene71718 | 0.859955 | 2.89E-63 | -1.69E-46 |
| MS.gene052611 | MS.gene71718 | 0.859392 | 4.27E-63 | -1.69E-46 |
| MS.gene052612 | MS.gene71718 | 0.802047 | 6.75E-49 | -1.69E-46 |
| MS.gene052773 | MS.gene71718 | 0.809951 | 1.47E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene71718 | 0.858281 | 9.17E-63 | -1.69E-46 |
| MS.gene052896 | MS.gene71718 | 0.80133 | 9.47E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene71718 | 0.815603 | 8.49E-52 | -1.69E-46 |
| MS.gene053145 | MS.gene71718 | 0.800141 | 1.66E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene71718 | 0.841772 | 3.88E-58 | -1.69E-46 |
| MS.gene053900 | MS.gene71718 | 0.818276 | 2.13E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene71718 | 0.813056 | 3.11E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene71718 | 0.856399 | 3.30E-62 | -1.69E-46 |
| MS.gene055818 | MS.gene71718 | 0.845885 | 3.07E-59 | -1.69E-46 |
| MS.gene056050 | MS.gene71718 | 0.818144 | 2.29E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene71718 | 0.832178 | 1.10E-55 | -1.69E-46 |
| MS.gene056424 | MS.gene71718 | 0.801835 | 7.46E-49 | -1.69E-46 |
| MS.gene056542 | MS.gene71718 | 0.833268 | 5.88E-56 | -1.69E-46 |
| MS.gene056543 | MS.gene71718 | 0.825584 | 4.33E-54 | -1.69E-46 |
| MS.gene056545 | MS.gene71718 | 0.836742 | 7.83E-57 | -1.69E-46 |
| MS.gene056553 | MS.gene71718 | 0.807336 | 5.31E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene71718 | 0.813677 | 2.27E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene71718 | 0.815113 | 1.09E-51 | -1.69E-46 |
| MS.gene057132 | MS.gene71718 | 0.827693 | 1.36E-54 | -1.69E-46 |
| MS.gene057855 | MS.gene71718 | 0.808283 | 3.34E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene71718 | 0.805914 | 1.06E-49 | -1.69E-46 |
| MS.gene058009 | MS.gene71718 | 0.814118 | 1.81E-51 | -1.69E-46 |
| MS.gene058010 | MS.gene71718 | 0.809372 | 1.96E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene71718 | 0.802299 | 5.99E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene71718 | 0.848755 | 5.01E-60 | -1.69E-46 |
| MS.gene058783 | MS.gene71718 | 0.828933 | 6.83E-55 | -1.69E-46 |
| MS.gene05894 | MS.gene71718 | 0.812423 | 4.27E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene71718 | 0.817513 | 3.17E-52 | -1.69E-46 |
| MS.gene059015 | MS.gene71718 | 0.806606 | 7.58E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene71718 | 0.801928 | 7.14E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene71718 | 0.828384 | 9.26E-55 | -1.69E-46 |
| MS.gene060352 | MS.gene71718 | 0.810722 | 1.00E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene71718 | 0.811094 | 8.31E-51 | -1.69E-46 |
| MS.gene060962 | MS.gene71718 | 0.808352 | 3.23E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71718.t1 | MTR_1g062090 | 86.017 | 236 | 14 | 1 | 1 | 236 | 139 | 355 | 9.05e-148 | 419 |
| MS.gene71718.t1 | MTR_4g060790 | 63.900 | 241 | 85 | 1 | 1 | 241 | 145 | 383 | 4.12e-109 | 322 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71718.t1 | AT5G60370 | 48.739 | 238 | 118 | 2 | 1 | 236 | 148 | 383 | 9.37e-68 | 216 |
| MS.gene71718.t1 | AT5G60370 | 52.830 | 159 | 71 | 2 | 1 | 157 | 148 | 304 | 6.14e-42 | 147 |
Find 49 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGCATCTTACACATATTT+TGG | 0.169674 | 1.1:-77503041 | None:intergenic |
| GCAAAATCAAAGCTTATTAT+AGG | 0.231984 | 1.1:-77502700 | None:intergenic |
| TTAGGAATTGTCTTGAGTTT+TGG | 0.264173 | 1.1:+77503169 | MS.gene71718:CDS |
| CGAACAGCGTGTGTTGATTC+TGG | 0.273513 | 1.1:+77502979 | MS.gene71718:CDS |
| ATCTTCCTCATCAACATAAC+TGG | 0.331924 | 1.1:-77503208 | None:intergenic |
| GGTGGATAAAGAATGAAATT+AGG | 0.335421 | 1.1:+77503151 | MS.gene71718:CDS |
| TCAGCTTGAACAAGAGGTTC+TGG | 0.351425 | 1.1:+77502567 | MS.gene71718:CDS |
| GTGTTCAATAAGTTCTGTTA+TGG | 0.366221 | 1.1:+77503339 | MS.gene71718:CDS |
| AAACTTGTTAATTTCATCAA+TGG | 0.388045 | 1.1:+77502637 | MS.gene71718:CDS |
| GAAGTCATCTGAAGACTATA+TGG | 0.392038 | 1.1:+77502609 | MS.gene71718:CDS |
| AGTTCTGTTATGGTTTCTGC+TGG | 0.409840 | 1.1:+77503349 | MS.gene71718:CDS |
| TGGTTTCTGCTGGAATGGTT+AGG | 0.414203 | 1.1:+77503359 | MS.gene71718:CDS |
| TGTTATGGTTTCTGCTGGAA+TGG | 0.419059 | 1.1:+77503354 | MS.gene71718:CDS |
| GACTTCACATTCACTTCCAC+TGG | 0.448189 | 1.1:-77502592 | None:intergenic |
| TGGGGAAAATTGATGAAATT+CGG | 0.453588 | 1.1:+77502740 | MS.gene71718:CDS |
| CACCAATGTAAGCAGGGCAC+TGG | 0.454243 | 1.1:-77503262 | None:intergenic |
| TAATGTGTTACAAGTATTTG+TGG | 0.476002 | 1.1:+77502869 | MS.gene71718:CDS |
| AGCATTGACACTTGCTGATG+TGG | 0.498297 | 1.1:+77503008 | MS.gene71718:CDS |
| CACAAGGTATTTGGATGGTG+GGG | 0.503716 | 1.1:+77502722 | MS.gene71718:CDS |
| GCACAAGGTATTTGGATGGT+GGG | 0.504127 | 1.1:+77502721 | MS.gene71718:CDS |
| TCAGAATCACCAATGTAAGC+AGG | 0.505369 | 1.1:-77503269 | None:intergenic |
| TCCCAGTGCCCTGCTTACAT+TGG | 0.505487 | 1.1:+77503260 | MS.gene71718:CDS |
| TGAGTCATCATACTCTGAAA+GGG | 0.510429 | 1.1:-77503303 | None:intergenic |
| CACTAGCTTATCATTAGCAC+GGG | 0.510557 | 1.1:-77503064 | None:intergenic |
| TTGAGTCATCATACTCTGAA+AGG | 0.523686 | 1.1:-77503304 | None:intergenic |
| TATTCAAACAATCTTTCAGA+AGG | 0.525726 | 1.1:-77502916 | None:intergenic |
| AATGTGTTACAAGTATTTGT+GGG | 0.525943 | 1.1:+77502870 | MS.gene71718:CDS |
| GTCTTAGTTTCAACTAGCAT+CGG | 0.533684 | 1.1:-77502790 | None:intergenic |
| AAGAGTAAAGCGATGAAAGC+TGG | 0.536293 | 1.1:+77502529 | None:intergenic |
| GTGAATCAACTGTTGTTTGA+AGG | 0.539135 | 1.1:+77502661 | MS.gene71718:CDS |
| TGCACAAGGTATTTGGATGG+TGG | 0.540504 | 1.1:+77502720 | MS.gene71718:CDS |
| AGAAGCCAGTTATGTTGATG+AGG | 0.540672 | 1.1:+77503203 | MS.gene71718:CDS |
| TGATGAAATTCGGATGCCTA+AGG | 0.544579 | 1.1:+77502750 | MS.gene71718:CDS |
| TCAGAGGGATCACTCACTGT+TGG | 0.546666 | 1.1:+77503101 | MS.gene71718:CDS |
| CGATGAAAGCTGGTATAGAT+CGG | 0.566862 | 1.1:+77502539 | None:intergenic |
| ACCAATGTAAGCAGGGCACT+GGG | 0.567241 | 1.1:-77503261 | None:intergenic |
| ATTCTGCAGGAACTGTATCT+CGG | 0.580937 | 1.1:-77502819 | None:intergenic |
| ATGTTGATGAGGAAGATCAA+TGG | 0.586177 | 1.1:+77503214 | MS.gene71718:CDS |
| ATTCAAACAATCTTTCAGAA+GGG | 0.587293 | 1.1:-77502915 | None:intergenic |
| ATTGACACTTGCTGATGTGG+TGG | 0.594742 | 1.1:+77503011 | MS.gene71718:CDS |
| ACACTAGCTTATCATTAGCA+CGG | 0.607468 | 1.1:-77503065 | None:intergenic |
| CAGAATCACCAATGTAAGCA+GGG | 0.612970 | 1.1:-77503268 | None:intergenic |
| TGAGGAAGATCAATGGAATG+TGG | 0.617405 | 1.1:+77503221 | MS.gene71718:CDS |
| GTTGAGATACGAATCTCAGA+GGG | 0.624591 | 1.1:+77503086 | MS.gene71718:CDS |
| ACAAGAGGTTCTGGAACCAG+TGG | 0.648454 | 1.1:+77502576 | MS.gene71718:CDS |
| AATTTGTGTATGATGGTAGG+TGG | 0.653126 | 1.1:+77503133 | MS.gene71718:CDS |
| TCTTTACATAAATTACGTCG+AGG | 0.667263 | 1.1:-77502952 | None:intergenic |
| TGTTGAGATACGAATCTCAG+AGG | 0.675787 | 1.1:+77503085 | MS.gene71718:CDS |
| ACTAGCTTATCATTAGCACG+GGG | 0.797096 | 1.1:-77503063 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAACTTGTTAATTTCATCAA+TGG | + | chr1.1:77502637-77502656 | MS.gene71718:CDS | 20.0% |
| ! | AAAAATTTGTGTATGATGGT+AGG | + | chr1.1:77503130-77503149 | MS.gene71718:CDS | 25.0% |
| ! | AATGTGTTACAAGTATTTGT+GGG | + | chr1.1:77502870-77502889 | MS.gene71718:CDS | 25.0% |
| ! | ATTCAAACAATCTTTCAGAA+GGG | - | chr1.1:77502918-77502937 | None:intergenic | 25.0% |
| ! | GAAGAAAAATTTGTGTATGA+TGG | + | chr1.1:77503126-77503145 | MS.gene71718:CDS | 25.0% |
| ! | GCAAAATCAAAGCTTATTAT+AGG | - | chr1.1:77502703-77502722 | None:intergenic | 25.0% |
| ! | TAATGTGTTACAAGTATTTG+TGG | + | chr1.1:77502869-77502888 | MS.gene71718:CDS | 25.0% |
| ! | TATTCAAACAATCTTTCAGA+AGG | - | chr1.1:77502919-77502938 | None:intergenic | 25.0% |
| !!! | TTATGATCTTTTTTTGCCTT+AGG | - | chr1.1:77502769-77502788 | None:intergenic | 25.0% |
| CAGAATCACAAAAAAGAAAC+GGG | + | chr1.1:77502836-77502855 | MS.gene71718:CDS | 30.0% | |
| GCAGAATCACAAAAAAGAAA+CGG | + | chr1.1:77502835-77502854 | MS.gene71718:CDS | 30.0% | |
| GGTGGATAAAGAATGAAATT+AGG | + | chr1.1:77503151-77503170 | MS.gene71718:CDS | 30.0% | |
| GTGTTCAATAAGTTCTGTTA+TGG | + | chr1.1:77503339-77503358 | MS.gene71718:CDS | 30.0% | |
| TCTTTACATAAATTACGTCG+AGG | - | chr1.1:77502955-77502974 | None:intergenic | 30.0% | |
| TGGGGAAAATTGATGAAATT+CGG | + | chr1.1:77502740-77502759 | MS.gene71718:CDS | 30.0% | |
| ! | TTAGGAATTGTCTTGAGTTT+TGG | + | chr1.1:77503169-77503188 | MS.gene71718:CDS | 30.0% |
| !! | ATAAGCTTTGATTTTGCACA+AGG | + | chr1.1:77502706-77502725 | MS.gene71718:CDS | 30.0% |
| !! | TTGATTTTGCACAAGGTATT+TGG | + | chr1.1:77502713-77502732 | MS.gene71718:CDS | 30.0% |
| !!! | TTTCTTTTTTGTGATTCTGC+AGG | - | chr1.1:77502835-77502854 | None:intergenic | 30.0% |
| AATCACAAAAAAGAAACGGG+AGG | + | chr1.1:77502839-77502858 | MS.gene71718:CDS | 35.0% | |
| AATTTGTGTATGATGGTAGG+TGG | + | chr1.1:77503133-77503152 | MS.gene71718:CDS | 35.0% | |
| ATCTTCCTCATCAACATAAC+TGG | - | chr1.1:77503211-77503230 | None:intergenic | 35.0% | |
| GAAGTCATCTGAAGACTATA+TGG | + | chr1.1:77502609-77502628 | MS.gene71718:CDS | 35.0% | |
| GTCTTAGTTTCAACTAGCAT+CGG | - | chr1.1:77502793-77502812 | None:intergenic | 35.0% | |
| TTGAGTCATCATACTCTGAA+AGG | - | chr1.1:77503307-77503326 | None:intergenic | 35.0% | |
| ! | ACACTAGCTTATCATTAGCA+CGG | - | chr1.1:77503068-77503087 | None:intergenic | 35.0% |
| ! | ATGTTGATGAGGAAGATCAA+TGG | + | chr1.1:77503214-77503233 | MS.gene71718:CDS | 35.0% |
| ! | GCAGCATCTTACACATATTT+TGG | - | chr1.1:77503044-77503063 | None:intergenic | 35.0% |
| ! | GTGAATCAACTGTTGTTTGA+AGG | + | chr1.1:77502661-77502680 | MS.gene71718:CDS | 35.0% |
| ! | TGAGTCATCATACTCTGAAA+GGG | - | chr1.1:77503306-77503325 | None:intergenic | 35.0% |
| !! | TTTTGCACAAGGTATTTGGA+TGG | + | chr1.1:77502717-77502736 | MS.gene71718:CDS | 35.0% |
| !!! | AATTGTCTTGAGTTTTGGCT+TGG | + | chr1.1:77503174-77503193 | MS.gene71718:CDS | 35.0% |
| AGAAGCCAGTTATGTTGATG+AGG | + | chr1.1:77503203-77503222 | MS.gene71718:CDS | 40.0% | |
| AGTTCTGTTATGGTTTCTGC+TGG | + | chr1.1:77503349-77503368 | MS.gene71718:CDS | 40.0% | |
| ATTCTGCAGGAACTGTATCT+CGG | - | chr1.1:77502822-77502841 | None:intergenic | 40.0% | |
| CAGAATCACCAATGTAAGCA+GGG | - | chr1.1:77503271-77503290 | None:intergenic | 40.0% | |
| GTTGAGATACGAATCTCAGA+GGG | + | chr1.1:77503086-77503105 | MS.gene71718:CDS | 40.0% | |
| TCAGAATCACCAATGTAAGC+AGG | - | chr1.1:77503272-77503291 | None:intergenic | 40.0% | |
| TGAGGAAGATCAATGGAATG+TGG | + | chr1.1:77503221-77503240 | MS.gene71718:CDS | 40.0% | |
| TGATGAAATTCGGATGCCTA+AGG | + | chr1.1:77502750-77502769 | MS.gene71718:CDS | 40.0% | |
| TGTTATGGTTTCTGCTGGAA+TGG | + | chr1.1:77503354-77503373 | MS.gene71718:CDS | 40.0% | |
| TGTTGAGATACGAATCTCAG+AGG | + | chr1.1:77503085-77503104 | MS.gene71718:CDS | 40.0% | |
| ! | ACTAGCTTATCATTAGCACG+GGG | - | chr1.1:77503066-77503085 | None:intergenic | 40.0% |
| ! | CACTAGCTTATCATTAGCAC+GGG | - | chr1.1:77503067-77503086 | None:intergenic | 40.0% |
| ! | GCATTTTCAGCTTGAACAAG+AGG | + | chr1.1:77502561-77502580 | MS.gene71718:CDS | 40.0% |
| GACTTCACATTCACTTCCAC+TGG | - | chr1.1:77502595-77502614 | None:intergenic | 45.0% | |
| TCAGCTTGAACAAGAGGTTC+TGG | + | chr1.1:77502567-77502586 | MS.gene71718:CDS | 45.0% | |
| ! | ATTGACACTTGCTGATGTGG+TGG | + | chr1.1:77503011-77503030 | MS.gene71718:CDS | 45.0% |
| ! | CACAAGGTATTTGGATGGTG+GGG | + | chr1.1:77502722-77502741 | MS.gene71718:CDS | 45.0% |
| ! | GCACAAGGTATTTGGATGGT+GGG | + | chr1.1:77502721-77502740 | MS.gene71718:CDS | 45.0% |
| ! | TGCACAAGGTATTTGGATGG+TGG | + | chr1.1:77502720-77502739 | MS.gene71718:CDS | 45.0% |
| !! | AGCATTGACACTTGCTGATG+TGG | + | chr1.1:77503008-77503027 | MS.gene71718:CDS | 45.0% |
| TCAGAGGGATCACTCACTGT+TGG | + | chr1.1:77503101-77503120 | MS.gene71718:CDS | 50.0% | |
| ! | ACCAATGTAAGCAGGGCACT+GGG | - | chr1.1:77503264-77503283 | None:intergenic | 50.0% |
| !! | ACAAGAGGTTCTGGAACCAG+TGG | + | chr1.1:77502576-77502595 | MS.gene71718:CDS | 50.0% |
| !! | CGAACAGCGTGTGTTGATTC+TGG | + | chr1.1:77502979-77502998 | MS.gene71718:CDS | 50.0% |
| CACCAATGTAAGCAGGGCAC+TGG | - | chr1.1:77503265-77503284 | None:intergenic | 55.0% | |
| TCCCAGTGCCCTGCTTACAT+TGG | + | chr1.1:77503260-77503279 | MS.gene71718:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 77502541 | 77503380 | 77502541 | ID=MS.gene71718 |
| chr1.1 | mRNA | 77502541 | 77503380 | 77502541 | ID=MS.gene71718.t1;Parent=MS.gene71718 |
| chr1.1 | exon | 77502541 | 77503380 | 77502541 | ID=MS.gene71718.t1.exon1;Parent=MS.gene71718.t1 |
| chr1.1 | CDS | 77502541 | 77503380 | 77502541 | ID=cds.MS.gene71718.t1;Parent=MS.gene71718.t1 |
| Gene Sequence |
| Protein sequence |
