Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72382.t1 | XP_002526487.1 | 100 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 1.30E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72382.t1 | Q9XHE4 | 97.8 | 92 | 2 | 0 | 1 | 92 | 1 | 92 | 5.9e-45 | 181.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72382.t1 | A0A251S2J9 | 100.0 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.4e-44 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene72382 | 0.815328 | 9.78E-52 | -1.69E-46 |
| MS.gene049387 | MS.gene72382 | 0.806789 | 6.93E-50 | -1.69E-46 |
| MS.gene050134 | MS.gene72382 | 0.885198 | 1.00E-71 | -1.69E-46 |
| MS.gene050252 | MS.gene72382 | 0.83199 | 1.22E-55 | -1.69E-46 |
| MS.gene050791 | MS.gene72382 | 0.891851 | 2.72E-74 | -1.69E-46 |
| MS.gene050912 | MS.gene72382 | 0.886227 | 4.11E-72 | -1.69E-46 |
| MS.gene051904 | MS.gene72382 | 0.864595 | 1.09E-64 | -1.69E-46 |
| MS.gene051984 | MS.gene72382 | 0.866779 | 2.22E-65 | -1.69E-46 |
| MS.gene052412 | MS.gene72382 | 0.807027 | 6.18E-50 | -1.69E-46 |
| MS.gene052664 | MS.gene72382 | 0.877262 | 7.23E-69 | -1.69E-46 |
| MS.gene053224 | MS.gene72382 | 0.86772 | 1.11E-65 | -1.69E-46 |
| MS.gene055232 | MS.gene72382 | 0.812506 | 4.10E-51 | -1.69E-46 |
| MS.gene056917 | MS.gene72382 | 0.802428 | 5.64E-49 | -1.69E-46 |
| MS.gene057092 | MS.gene72382 | 0.821147 | 4.71E-53 | -1.69E-46 |
| MS.gene057235 | MS.gene72382 | 0.811665 | 6.25E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene72382 | 0.876141 | 1.77E-68 | -1.69E-46 |
| MS.gene057641 | MS.gene72382 | 0.860321 | 2.24E-63 | -1.69E-46 |
| MS.gene057643 | MS.gene72382 | 0.891548 | 3.59E-74 | -1.69E-46 |
| MS.gene059340 | MS.gene72382 | 0.804741 | 1.87E-49 | -1.69E-46 |
| MS.gene060483 | MS.gene72382 | 0.835502 | 1.62E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72382.t1 | MTR_4g099160 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.47e-63 | 185 |
| MS.gene72382.t1 | MTR_4g103340 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.47e-63 | 185 |
| MS.gene72382.t1 | MTR_8g007485 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.47e-63 | 185 |
| MS.gene72382.t1 | MTR_0199s0040 | 100.000 | 92 | 0 | 0 | 1 | 92 | 82 | 173 | 1.46e-62 | 187 |
| MS.gene72382.t1 | MTR_4g103340 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 9.22e-47 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72382.t1 | AT3G10950 | 93.478 | 92 | 6 | 0 | 1 | 92 | 1 | 92 | 4.32e-59 | 176 |
| MS.gene72382.t1 | AT3G60245 | 92.308 | 91 | 7 | 0 | 1 | 91 | 1 | 91 | 8.36e-58 | 172 |
Find 23 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAAAGGCTGTAGGAATA+TGG | 0.316747 | 8.3:-21489449 | MS.gene72382:CDS |
| GAGAAAGGCTGTAGGAATAT+GGG | 0.416937 | 8.3:-21489448 | MS.gene72382:CDS |
| GCTGGTATCGTTGGCAAATA+CGG | 0.433650 | 8.3:-21490341 | MS.gene72382:intron |
| ATGATATTAGGTACCCGTTA+TGG | 0.441488 | 8.3:-21489672 | MS.gene72382:intron |
| CTGCAAGTGCCGTGACTGTT+CGG | 0.445396 | 8.3:-21489289 | MS.gene72382:CDS |
| TGACTGTTCGGAGCACCATC+AGG | 0.472923 | 8.3:-21489277 | MS.gene72382:CDS |
| ACGCAAACTAGCACCATAAC+GGG | 0.476774 | 8.3:+21489659 | None:intergenic |
| TTGTTCCCTCAACCTCCTGA+TGG | 0.500191 | 8.3:+21489262 | None:intergenic |
| GGAGCACCATCAGGAGGTTG+AGG | 0.503709 | 8.3:-21489268 | MS.gene72382:CDS |
| TACGCAAACTAGCACCATAA+CGG | 0.535734 | 8.3:+21489658 | None:intergenic |
| AGAAAGGCTGTAGGAATATG+GGG | 0.537082 | 8.3:-21489447 | MS.gene72382:CDS |
| ACTAAGAGAACTAAGAAGGC+TGG | 0.546098 | 8.3:-21490359 | MS.gene72382:CDS |
| GAGCACCATCAGGAGGTTGA+GGG | 0.548544 | 8.3:-21489267 | MS.gene72382:CDS |
| ACTAAGAAGGCTGGTATCGT+TGG | 0.554051 | 8.3:-21490350 | MS.gene72382:CDS |
| GCAGACTAAGAGAACTAAGA+AGG | 0.566891 | 8.3:-21490363 | MS.gene72382:intron |
| GATTGCGGTAAAGTGAAAGC+AGG | 0.587249 | 8.3:-21489417 | MS.gene72382:CDS |
| TGTAGGAATATGGGGATGCA+AGG | 0.599232 | 8.3:-21489439 | MS.gene72382:CDS |
| GATGGTGCTCCGAACAGTCA+CGG | 0.619064 | 8.3:+21489280 | None:intergenic |
| GCAGTATGCTGTGAAGAGAA+AGG | 0.632531 | 8.3:-21489463 | MS.gene72382:intron |
| TGCGGTAAAGTGAAAGCAGG+CGG | 0.647382 | 8.3:-21489414 | MS.gene72382:CDS |
| ATATGGGGATGCAAGGATTG+CGG | 0.650089 | 8.3:-21489432 | MS.gene72382:CDS |
| GCTGTGAAGAGAAAGGCTGT+AGG | 0.663437 | 8.3:-21489456 | MS.gene72382:CDS |
| CTGTTCGGAGCACCATCAGG+AGG | 0.673364 | 8.3:-21489274 | MS.gene72382:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAATAATATTTTATGCA+AGG | - | chr8.3:21489816-21489835 | MS.gene72382:intron | 10.0% |
| !!! | ATTATATTTTATGTAAAACA+AGG | + | chr8.3:21490047-21490066 | None:intergenic | 10.0% |
| !!! | TGATTTAAATGTTTTAAATA+TGG | - | chr8.3:21489564-21489583 | MS.gene72382:intron | 10.0% |
| !!! | TTTTATTTTTCAAATCTGAA+AGG | - | chr8.3:21490457-21490476 | MS.gene72382:intron | 15.0% |
| !! | ACAAAGAATTTGTAAGATTT+CGG | + | chr8.3:21489496-21489515 | None:intergenic | 20.0% |
| !! | ACAAATACAATTCATGAATT+GGG | + | chr8.3:21490013-21490032 | None:intergenic | 20.0% |
| !! | GAAAAATAAAACATTGAGAA+AGG | + | chr8.3:21490448-21490467 | None:intergenic | 20.0% |
| !! | TACAAATACAATTCATGAAT+TGG | + | chr8.3:21490014-21490033 | None:intergenic | 20.0% |
| !! | TATACATGGATATATGTATA+TGG | - | chr8.3:21489701-21489720 | MS.gene72382:intron | 20.0% |
| !!! | AAATATGGTTTTGTTTATGA+TGG | - | chr8.3:21489579-21489598 | MS.gene72382:intron | 20.0% |
| !!! | AAATTTTTCTGTGAATTTTG+TGG | - | chr8.3:21490206-21490225 | MS.gene72382:intron | 20.0% |
| !!! | ATCTAAGTATTTAGTTTAGT+TGG | - | chr8.3:21490270-21490289 | MS.gene72382:intron | 20.0% |
| !!! | TGGTTTTATTGTATGATATT+AGG | - | chr8.3:21490122-21490141 | MS.gene72382:intron | 20.0% |
| ! | AAAACATTGAGAAAGGAATA+AGG | + | chr8.3:21490441-21490460 | None:intergenic | 25.0% |
| ! | AAAATAAACCAATCCAAACT+AGG | + | chr8.3:21489770-21489789 | None:intergenic | 25.0% |
| ! | ATGATATACAACATTACACA+AGG | + | chr8.3:21490241-21490260 | None:intergenic | 25.0% |
| ! | GATTTATTTGTAACTCTTCA+AGG | - | chr8.3:21490070-21490089 | MS.gene72382:intron | 25.0% |
| ! | TTTGTGAGAAGTTAATTCAT+TGG | - | chr8.3:21490102-21490121 | MS.gene72382:intron | 25.0% |
| !! | TTGAAAGAGAAGTTTAGAAT+TGG | + | chr8.3:21489303-21489322 | None:intergenic | 25.0% |
| !!! | TTGGTTTATTTTTGAGTTTG+TGG | - | chr8.3:21489778-21489797 | MS.gene72382:intron | 25.0% |
| ACCTAAAATGTAACTACCTA+GGG | + | chr8.3:21489948-21489967 | None:intergenic | 30.0% | |
| ATAATGAGAACAAAGTCATG+AGG | + | chr8.3:21490487-21490506 | None:intergenic | 30.0% | |
| TTACAAATCTGAGTGAACAA+AGG | + | chr8.3:21489346-21489365 | None:intergenic | 30.0% | |
| ! | AGAGAAGTTTAGAATTGGAT+TGG | + | chr8.3:21489298-21489317 | None:intergenic | 30.0% |
| ! | TTTAGAATTGGATTGGATGA+TGG | + | chr8.3:21489291-21489310 | None:intergenic | 30.0% |
| ! | TTTAGGTGGTTTGATGTTTT+GGG | - | chr8.3:21489961-21489980 | MS.gene72382:intron | 30.0% |
| ! | TTTCTGTGAATTTTGTGGAA+AGG | - | chr8.3:21490211-21490230 | MS.gene72382:intron | 30.0% |
| !! | TTTTAGGTGGTTTGATGTTT+TGG | - | chr8.3:21489960-21489979 | MS.gene72382:intron | 30.0% |
| !!! | TAAGCTATTTTTCATAAGCC+AGG | - | chr8.3:21489922-21489941 | MS.gene72382:intron | 30.0% |
| ATGATATTAGGTACCCGTTA+TGG | - | chr8.3:21490134-21490153 | MS.gene72382:intron | 35.0% | |
| CACCTAAAATGTAACTACCT+AGG | + | chr8.3:21489949-21489968 | None:intergenic | 35.0% | |
| GCGTAAGCAGATTAAAAAGA+TGG | - | chr8.3:21490166-21490185 | MS.gene72382:intron | 35.0% | |
| GGTAATTGATCATCAGAACT+CGG | + | chr8.3:21490319-21490338 | None:intergenic | 35.0% | |
| TTGTCTTGCACTCTTATACA+TGG | - | chr8.3:21489687-21489706 | MS.gene72382:intron | 35.0% | |
| ! | CTAGGTAGTTACATTTTAGG+TGG | - | chr8.3:21489947-21489966 | MS.gene72382:intron | 35.0% |
| ! | TTAGGTGGTTTGATGTTTTG+GGG | - | chr8.3:21489962-21489981 | MS.gene72382:intron | 35.0% |
| !! | ATTGGATTGGATGATGGAAA+AGG | + | chr8.3:21489285-21489304 | None:intergenic | 35.0% |
| !! | TTGGATTGGATGATGGAAAA+GGG | + | chr8.3:21489284-21489303 | None:intergenic | 35.0% |
| ACTAAGAGAACTAAGAAGGC+TGG | - | chr8.3:21489447-21489466 | MS.gene72382:CDS | 40.0% | |
| AGAAAGGCTGTAGGAATATG+GGG | - | chr8.3:21490359-21490378 | MS.gene72382:CDS | 40.0% | |
| AGAGAAAGGCTGTAGGAATA+TGG | - | chr8.3:21490357-21490376 | MS.gene72382:CDS | 40.0% | |
| GAGAAAGGCTGTAGGAATAT+GGG | - | chr8.3:21490358-21490377 | MS.gene72382:CDS | 40.0% | |
| GCAGACTAAGAGAACTAAGA+AGG | - | chr8.3:21489443-21489462 | MS.gene72382:CDS | 40.0% | |
| TACGCAAACTAGCACCATAA+CGG | + | chr8.3:21490151-21490170 | None:intergenic | 40.0% | |
| TGACTACACCTAGTTTGGAT+TGG | - | chr8.3:21489759-21489778 | MS.gene72382:intron | 40.0% | |
| ! | GCCCTAGGTAGTTACATTTT+AGG | - | chr8.3:21489944-21489963 | MS.gene72382:intron | 40.0% |
| !! | CAGAACTCGGCATTTTGAAA+AGG | + | chr8.3:21490306-21490325 | None:intergenic | 40.0% |
| !! | TAGGTGGTTTGATGTTTTGG+GGG | - | chr8.3:21489963-21489982 | MS.gene72382:intron | 40.0% |
| !! | TGGATTGGATGATGGAAAAG+GGG | + | chr8.3:21489283-21489302 | None:intergenic | 40.0% |
| AAATGTAACTACCTAGGGCC+TGG | + | chr8.3:21489943-21489962 | None:intergenic | 45.0% | |
| ACGCAAACTAGCACCATAAC+GGG | + | chr8.3:21490150-21490169 | None:intergenic | 45.0% | |
| ACTAAGAAGGCTGGTATCGT+TGG | - | chr8.3:21489456-21489475 | MS.gene72382:CDS | 45.0% | |
| ATATGGGGATGCAAGGATTG+CGG | - | chr8.3:21490374-21490393 | MS.gene72382:intron | 45.0% | |
| GATTGCGGTAAAGTGAAAGC+AGG | - | chr8.3:21490389-21490408 | MS.gene72382:intron | 45.0% | |
| GCAGTATGCTGTGAAGAGAA+AGG | - | chr8.3:21490343-21490362 | MS.gene72382:CDS | 45.0% | |
| GCTGGTATCGTTGGCAAATA+CGG | - | chr8.3:21489465-21489484 | MS.gene72382:intron | 45.0% | |
| GTAGCTGACTACACCTAGTT+TGG | - | chr8.3:21489754-21489773 | MS.gene72382:intron | 45.0% | |
| TCTTCACAGCATACTGCAAC+AGG | + | chr8.3:21490340-21490359 | None:intergenic | 45.0% | |
| TGAGAACAAAGTCATGAGGC+TGG | + | chr8.3:21490483-21490502 | None:intergenic | 45.0% | |
| TGTAGGAATATGGGGATGCA+AGG | - | chr8.3:21490367-21490386 | MS.gene72382:intron | 45.0% | |
| ! | ATGATGGAAAAGGGGAGAAG+GGG | + | chr8.3:21489275-21489294 | None:intergenic | 45.0% |
| ! | TTTTTCATAAGCCAGGCCCT+AGG | - | chr8.3:21489929-21489948 | MS.gene72382:intron | 45.0% |
| !! | GATGATGGAAAAGGGGAGAA+GGG | + | chr8.3:21489276-21489295 | None:intergenic | 45.0% |
| AAAAGGGGAGAAGGGGAGAA+GGG | + | chr8.3:21489268-21489287 | None:intergenic | 50.0% | |
| GCTGTGAAGAGAAAGGCTGT+AGG | - | chr8.3:21490350-21490369 | MS.gene72382:CDS | 50.0% | |
| TGCGGTAAAGTGAAAGCAGG+CGG | - | chr8.3:21490392-21490411 | MS.gene72382:intron | 50.0% | |
| TTGTTCCCTCAACCTCCTGA+TGG | + | chr8.3:21490547-21490566 | None:intergenic | 50.0% | |
| !! | GGATGATGGAAAAGGGGAGA+AGG | + | chr8.3:21489277-21489296 | None:intergenic | 50.0% |
| AAAGGGGAGAAGGGGAGAAG+GGG | + | chr8.3:21489267-21489286 | None:intergenic | 55.0% | |
| GAAAAGGGGAGAAGGGGAGA+AGG | + | chr8.3:21489269-21489288 | None:intergenic | 55.0% | |
| GAGCACCATCAGGAGGTTGA+GGG | - | chr8.3:21490539-21490558 | MS.gene72382:intron | 55.0% | |
| GATGGTGCTCCGAACAGTCA+CGG | + | chr8.3:21490529-21490548 | None:intergenic | 55.0% | |
| ! | CTGCAAGTGCCGTGACTGTT+CGG | - | chr8.3:21490517-21490536 | MS.gene72382:intron | 55.0% |
| ! | TGACTGTTCGGAGCACCATC+AGG | - | chr8.3:21490529-21490548 | MS.gene72382:intron | 55.0% |
| ! | CTGTTCGGAGCACCATCAGG+AGG | - | chr8.3:21490532-21490551 | MS.gene72382:intron | 60.0% |
| ! | GGAGCACCATCAGGAGGTTG+AGG | - | chr8.3:21490538-21490557 | MS.gene72382:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 21489250 | 21490578 | 21489250 | ID=MS.gene72382 |
| chr8.3 | mRNA | 21489250 | 21490578 | 21489250 | ID=MS.gene72382.t1;Parent=MS.gene72382 |
| chr8.3 | exon | 21490576 | 21490578 | 21490576 | ID=MS.gene72382.t1.exon1;Parent=MS.gene72382.t1 |
| chr8.3 | CDS | 21490576 | 21490578 | 21490576 | ID=cds.MS.gene72382.t1;Parent=MS.gene72382.t1 |
| chr8.3 | exon | 21490342 | 21490381 | 21490342 | ID=MS.gene72382.t1.exon2;Parent=MS.gene72382.t1 |
| chr8.3 | CDS | 21490342 | 21490381 | 21490342 | ID=cds.MS.gene72382.t1;Parent=MS.gene72382.t1 |
| chr8.3 | exon | 21489596 | 21489684 | 21489596 | ID=MS.gene72382.t1.exon3;Parent=MS.gene72382.t1 |
| chr8.3 | CDS | 21489596 | 21489684 | 21489596 | ID=cds.MS.gene72382.t1;Parent=MS.gene72382.t1 |
| chr8.3 | exon | 21489399 | 21489481 | 21489399 | ID=MS.gene72382.t1.exon4;Parent=MS.gene72382.t1 |
| chr8.3 | CDS | 21489399 | 21489481 | 21489399 | ID=cds.MS.gene72382.t1;Parent=MS.gene72382.t1 |
| chr8.3 | exon | 21489250 | 21489313 | 21489250 | ID=MS.gene72382.t1.exon5;Parent=MS.gene72382.t1 |
| chr8.3 | CDS | 21489250 | 21489313 | 21489250 | ID=cds.MS.gene72382.t1;Parent=MS.gene72382.t1 |
| Gene Sequence |
| Protein sequence |
