Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72747.t1 | XP_003612495.2 | 89.9 | 109 | 11 | 0 | 9 | 117 | 17 | 125 | 5.50E-34 | 153.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72747.t1 | G7K421 | 89.9 | 109 | 11 | 0 | 9 | 117 | 17 | 125 | 4.0e-34 | 153.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049488 | MS.gene72747 | 0.808388 | 3.17E-50 | -1.69E-46 |
| MS.gene049993 | MS.gene72747 | 0.811587 | 6.50E-51 | -1.69E-46 |
| MS.gene050670 | MS.gene72747 | 0.827319 | 1.67E-54 | -1.69E-46 |
| MS.gene051525 | MS.gene72747 | 0.811554 | 6.61E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene72747 | -0.803444 | 3.48E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene72747 | 0.805021 | 1.63E-49 | -1.69E-46 |
| MS.gene052253 | MS.gene72747 | 0.812999 | 3.20E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene72747 | 0.810435 | 1.15E-50 | -1.69E-46 |
| MS.gene052485 | MS.gene72747 | 0.81364 | 2.31E-51 | -1.69E-46 |
| MS.gene053813 | MS.gene72747 | 0.810203 | 1.30E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene72747 | 0.836951 | 6.92E-57 | -1.69E-46 |
| MS.gene054349 | MS.gene72747 | 0.807339 | 5.31E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene72747 | 0.836407 | 9.53E-57 | -1.69E-46 |
| MS.gene055369 | MS.gene72747 | 0.814001 | 1.92E-51 | -1.69E-46 |
| MS.gene055911 | MS.gene72747 | 0.807076 | 6.03E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene72747 | 0.807345 | 5.29E-50 | -1.69E-46 |
| MS.gene056407 | MS.gene72747 | 0.805803 | 1.12E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene72747 | 0.819716 | 1.00E-52 | -1.69E-46 |
| MS.gene056514 | MS.gene72747 | 0.807567 | 4.75E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene72747 | -0.807602 | 4.66E-50 | -1.69E-46 |
| MS.gene057749 | MS.gene72747 | 0.848808 | 4.84E-60 | -1.69E-46 |
| MS.gene057823 | MS.gene72747 | 0.845109 | 4.98E-59 | -1.69E-46 |
| MS.gene057824 | MS.gene72747 | 0.817894 | 2.60E-52 | -1.69E-46 |
| MS.gene057825 | MS.gene72747 | 0.809722 | 1.64E-50 | -1.69E-46 |
| MS.gene057826 | MS.gene72747 | 0.800769 | 1.23E-48 | -1.69E-46 |
| MS.gene057888 | MS.gene72747 | 0.815721 | 7.99E-52 | -1.69E-46 |
| MS.gene05886 | MS.gene72747 | 0.808867 | 2.51E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene72747 | 0.813589 | 2.37E-51 | -1.69E-46 |
| MS.gene058949 | MS.gene72747 | 0.801965 | 7.02E-49 | -1.69E-46 |
| MS.gene058995 | MS.gene72747 | 0.818175 | 2.25E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene72747 | -0.801132 | 1.04E-48 | -1.69E-46 |
| MS.gene05907 | MS.gene72747 | 0.809147 | 2.18E-50 | -1.69E-46 |
| MS.gene059082 | MS.gene72747 | 0.815956 | 7.09E-52 | -1.69E-46 |
| MS.gene059285 | MS.gene72747 | 0.804298 | 2.31E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene72747 | 0.860042 | 2.72E-63 | -1.69E-46 |
| MS.gene059462 | MS.gene72747 | 0.817295 | 3.55E-52 | -1.69E-46 |
| MS.gene059807 | MS.gene72747 | 0.820763 | 5.78E-53 | -1.69E-46 |
| MS.gene059876 | MS.gene72747 | 0.826109 | 3.25E-54 | -1.69E-46 |
| MS.gene060403 | MS.gene72747 | 0.801631 | 8.22E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene72747 | 0.800818 | 1.21E-48 | -1.69E-46 |
| MS.gene061159 | MS.gene72747 | 0.82468 | 7.08E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72747.t1 | MTR_5g025650 | 90.265 | 113 | 11 | 0 | 5 | 117 | 13 | 125 | 5.70e-64 | 207 |
| MS.gene72747.t1 | MTR_8g072210 | 48.214 | 112 | 53 | 1 | 3 | 114 | 1 | 107 | 3.13e-21 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTATTTCAGCTTCCATGGC+TGG | 0.407420 | 5.3:-15249054 | MS.gene72747:CDS |
| TAGAAAGGATAGTTTCAGAT+TGG | 0.419331 | 5.3:-15249298 | MS.gene72747:CDS |
| GGAAGCTGAAATACGGTAGA+AGG | 0.433466 | 5.3:+15249062 | None:intergenic |
| AGAAAGGATAGTTTCAGATT+GGG | 0.448247 | 5.3:-15249297 | MS.gene72747:CDS |
| CTACCGTATTTCAGCTTCCA+TGG | 0.449137 | 5.3:-15249058 | MS.gene72747:CDS |
| GGAACAAAGTCATTGATAGA+AGG | 0.463513 | 5.3:+15249114 | None:intergenic |
| TAGGAAGGGTAGCTTTAGAA+AGG | 0.469277 | 5.3:-15249313 | MS.gene72747:CDS |
| GACATGGAGAATAGTAATAG+TGG | 0.471196 | 5.3:-15249360 | MS.gene72747:CDS |
| GATTCGTCGTCGTCGGAGAC+AGG | 0.475074 | 5.3:+15249093 | None:intergenic |
| TCACAAACTCTCTTTCTGTT+AGG | 0.475121 | 5.3:-15249176 | MS.gene72747:CDS |
| CAGCCATGGAAGCTGAAATA+CGG | 0.530516 | 5.3:+15249055 | None:intergenic |
| GATCACACAGTGGTTGAGCT+AGG | 0.545146 | 5.3:-15249267 | MS.gene72747:CDS |
| AAGGATAGTTTCAGATTGGG+AGG | 0.546950 | 5.3:-15249294 | MS.gene72747:CDS |
| ACGTTCAGGTGTTCCAGCCA+TGG | 0.550679 | 5.3:+15249041 | None:intergenic |
| AGTATCTGAGAGAGCAGTGA+GGG | 0.572897 | 5.3:+15249143 | None:intergenic |
| GTTGTTTATGTGCAGCTTGG+GGG | 0.591555 | 5.3:-15248265 | MS.gene72747:intron |
| AAAAGTGGTAGCTTTAGGAA+GGG | 0.598113 | 5.3:-15249327 | MS.gene72747:CDS |
| ATTATAGAGCAACATGGACA+TGG | 0.609231 | 5.3:-15249376 | None:intergenic |
| AGCAGCGGATTCGTCGTCGT+CGG | 0.630964 | 5.3:+15249086 | None:intergenic |
| TAGTATCTGAGAGAGCAGTG+AGG | 0.721002 | 5.3:+15249142 | None:intergenic |
| AATACGGTAGAAGGTAGCAG+CGG | 0.736624 | 5.3:+15249071 | None:intergenic |
| GGGAGGAAAAGATCACACAG+TGG | 0.818391 | 5.3:-15249277 | MS.gene72747:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATAAGTTTTTATATATAG+TGG | - | chr5.3:15248791-15248810 | MS.gene72747:intron | 10.0% |
| !!! | GATATATATTTTTAATTAGA+AGG | - | chr5.3:15249254-15249273 | MS.gene72747:CDS | 10.0% |
| !!! | TATATTAAATAATTTTAACC+GGG | + | chr5.3:15248674-15248693 | None:intergenic | 10.0% |
| !!! | ATATTAAATAATTTTAACCG+GGG | + | chr5.3:15248673-15248692 | None:intergenic | 15.0% |
| !!! | TTTTATGTCTTAAATTTTGT+TGG | - | chr5.3:15249036-15249055 | MS.gene72747:intron | 15.0% |
| !!! | ATGAATTAATGTTTTGGTTT+GGG | - | chr5.3:15249329-15249348 | MS.gene72747:CDS | 20.0% |
| !!! | TATGAATTAATGTTTTGGTT+TGG | - | chr5.3:15249328-15249347 | MS.gene72747:CDS | 20.0% |
| !!! | TATTAAATAATTTTAACCGG+GGG | + | chr5.3:15248672-15248691 | None:intergenic | 20.0% |
| !!! | TGTTGTATGAATTAATGTTT+TGG | - | chr5.3:15249323-15249342 | MS.gene72747:CDS | 20.0% |
| ! | AAATAAAAAGTGGTAGCTTT+AGG | - | chr5.3:15248284-15248303 | MS.gene72747:intron | 25.0% |
| !! | ACACAAAAACTTCTTTTAGA+AGG | - | chr5.3:15248382-15248401 | MS.gene72747:intron | 25.0% |
| !!! | TAAAGCTACCACTTTTTATT+TGG | + | chr5.3:15248285-15248304 | None:intergenic | 25.0% |
| AGAAAGGATAGTTTCAGATT+GGG | - | chr5.3:15248319-15248338 | MS.gene72747:intron | 30.0% | |
| AGTGGAAACCAAATAAAAAG+TGG | - | chr5.3:15248274-15248293 | MS.gene72747:intron | 30.0% | |
| TAGAAAGGATAGTTTCAGAT+TGG | - | chr5.3:15248318-15248337 | MS.gene72747:intron | 30.0% | |
| !! | CAAAAACTTCTTTTAGAAGG+TGG | - | chr5.3:15248385-15248404 | MS.gene72747:intron | 30.0% |
| !! | CTAATAAAGTATCGCAATTG+TGG | - | chr5.3:15249178-15249197 | MS.gene72747:CDS | 30.0% |
| !!! | TATTTTTTCTCTTAAACCCC+CGG | - | chr5.3:15248653-15248672 | MS.gene72747:intron | 30.0% |
| ATGGGAAAATATACACGTTC+AGG | + | chr5.3:15248592-15248611 | None:intergenic | 35.0% | |
| GACATGGAGAATAGTAATAG+TGG | - | chr5.3:15248256-15248275 | MS.gene72747:intron | 35.0% | |
| GGAACAAAGTCATTGATAGA+AGG | + | chr5.3:15248505-15248524 | None:intergenic | 35.0% | |
| TCACAAACTCTCTTTCTGTT+AGG | - | chr5.3:15248440-15248459 | MS.gene72747:intron | 35.0% | |
| !! | AAAAAGTGGTAGCTTTAGGA+AGG | - | chr5.3:15248288-15248307 | MS.gene72747:intron | 35.0% |
| !! | AAAAGTGGTAGCTTTAGGAA+GGG | - | chr5.3:15248289-15248308 | MS.gene72747:intron | 35.0% |
| !! | AAACTTCTTTTAGAAGGTGG+TGG | - | chr5.3:15248388-15248407 | MS.gene72747:intron | 35.0% |
| !! | AAAGTATCGCAATTGTGGAT+TGG | - | chr5.3:15249183-15249202 | MS.gene72747:CDS | 35.0% |
| AAGGATAGTTTCAGATTGGG+AGG | - | chr5.3:15248322-15248341 | MS.gene72747:intron | 40.0% | |
| GCAGCTAGCAAGGTATATAT+GGG | + | chr5.3:15248610-15248629 | None:intergenic | 40.0% | |
| TGCAGCTAGCAAGGTATATA+TGG | + | chr5.3:15248611-15248630 | None:intergenic | 40.0% | |
| TTTATGAGTTGCAGCTAGCA+AGG | + | chr5.3:15248620-15248639 | None:intergenic | 40.0% | |
| !! | TAGGAAGGGTAGCTTTAGAA+AGG | - | chr5.3:15248303-15248322 | MS.gene72747:intron | 40.0% |
| AATACGGTAGAAGGTAGCAG+CGG | + | chr5.3:15248548-15248567 | None:intergenic | 45.0% | |
| AGTATCTGAGAGAGCAGTGA+GGG | + | chr5.3:15248476-15248495 | None:intergenic | 45.0% | |
| CAGCCATGGAAGCTGAAATA+CGG | + | chr5.3:15248564-15248583 | None:intergenic | 45.0% | |
| CTACCGTATTTCAGCTTCCA+TGG | - | chr5.3:15248558-15248577 | MS.gene72747:intron | 45.0% | |
| GGAAGCTGAAATACGGTAGA+AGG | + | chr5.3:15248557-15248576 | None:intergenic | 45.0% | |
| GGGTTGTTTATGTGCAGCTT+GGG | - | chr5.3:15249349-15249368 | MS.gene72747:CDS | 45.0% | |
| GGTTGTTTATGTGCAGCTTG+GGG | - | chr5.3:15249350-15249369 | MS.gene72747:CDS | 45.0% | |
| GTTGAAGTTCATGCAGTTGC+AGG | + | chr5.3:15249108-15249127 | None:intergenic | 45.0% | |
| GTTGTTTATGTGCAGCTTGG+GGG | - | chr5.3:15249351-15249370 | MS.gene72747:CDS | 45.0% | |
| TAGTATCTGAGAGAGCAGTG+AGG | + | chr5.3:15248477-15248496 | None:intergenic | 45.0% | |
| TGGGTTGTTTATGTGCAGCT+TGG | - | chr5.3:15249348-15249367 | MS.gene72747:CDS | 45.0% | |
| !! | CTTCTTTTAGAAGGTGGTGG+AGG | - | chr5.3:15248391-15248410 | MS.gene72747:intron | 45.0% |
| !! | TATATATGTAATAAATATAT+AGG | + | chr5.3:15248698-15248717 | None:intergenic | 5.0% |
| !! | TGATATAAAAAAAAAAAAAT+AGG | - | chr5.3:15248966-15248985 | MS.gene72747:intron | 5.0% |
| !! | TTGATATAATAAAAAAAAAT+AGG | - | chr5.3:15248931-15248950 | MS.gene72747:intron | 5.0% |
| !!! | ATATATCATTAATTTTAAAA+GGG | + | chr5.3:15249241-15249260 | None:intergenic | 5.0% |
| !!! | ATATATTAAATAATTTTAAC+CGG | + | chr5.3:15248675-15248694 | None:intergenic | 5.0% |
| !!! | TATATATCATTAATTTTAAA+AGG | + | chr5.3:15249242-15249261 | None:intergenic | 5.0% |
| CGTATTTCAGCTTCCATGGC+TGG | - | chr5.3:15248562-15248581 | MS.gene72747:intron | 50.0% | |
| GATCACACAGTGGTTGAGCT+AGG | - | chr5.3:15248349-15248368 | MS.gene72747:intron | 50.0% | |
| GGGAGGAAAAGATCACACAG+TGG | - | chr5.3:15248339-15248358 | MS.gene72747:intron | 50.0% | |
| ACGTTCAGGTGTTCCAGCCA+TGG | + | chr5.3:15248578-15248597 | None:intergenic | 55.0% | |
| GATTCGTCGTCGTCGGAGAC+AGG | + | chr5.3:15248526-15248545 | None:intergenic | 60.0% | |
| ! | AGCAGCGGATTCGTCGTCGT+CGG | + | chr5.3:15248533-15248552 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 15248253 | 15249385 | 15248253 | ID=MS.gene72747 |
| chr5.3 | mRNA | 15248253 | 15249385 | 15248253 | ID=MS.gene72747.t1;Parent=MS.gene72747 |
| chr5.3 | exon | 15249043 | 15249385 | 15249043 | ID=MS.gene72747.t1.exon1;Parent=MS.gene72747.t1 |
| chr5.3 | CDS | 15249043 | 15249385 | 15249043 | ID=cds.MS.gene72747.t1;Parent=MS.gene72747.t1 |
| chr5.3 | exon | 15248253 | 15248272 | 15248253 | ID=MS.gene72747.t1.exon2;Parent=MS.gene72747.t1 |
| chr5.3 | CDS | 15248253 | 15248272 | 15248253 | ID=cds.MS.gene72747.t1;Parent=MS.gene72747.t1 |
| Gene Sequence |
| Protein sequence |
