AlfalfaGEDB | MS.gene057824

M. sativa cultivar XinJiangDaYe / MS.gene057824

KEGG: -
GO: -
PFAM: PF19055.3

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057824.t1	XP_013456597.1	100	69	0	0	1	69	276	344	2.30E-32	147.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057824.t1	Q9C8K2	91.3	69	6	0	1	69	228	296	2.0e-32	139.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057824.t1	G7JRM3	100.0	69	0	0	1	69	236	304	1.7e-32	147.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054173	MS.gene057824	0.803115	4.07E-49	-1.69E-46
MS.gene054183	MS.gene057824	0.810909	9.12E-51	-1.69E-46
MS.gene057749	MS.gene057824	0.896901	2.36E-76	-1.69E-46
MS.gene057823	MS.gene057824	0.889506	2.28E-73	-1.69E-46
MS.gene057824	MS.gene057825	0.814846	1.25E-51	-1.69E-46
MS.gene057824	MS.gene057826	0.906407	1.53E-80	-1.69E-46
MS.gene057824	MS.gene058949	0.814565	1.44E-51	-1.69E-46
MS.gene057824	MS.gene059420	0.830068	3.62E-55	-1.69E-46
MS.gene057824	MS.gene064519	0.82348	1.35E-53	-1.69E-46
MS.gene057824	MS.gene065130	0.825852	3.74E-54	-1.69E-46
MS.gene057824	MS.gene067385	0.802077	6.66E-49	-1.69E-46
MS.gene057824	MS.gene069680	0.808782	2.61E-50	-1.69E-46
MS.gene057824	MS.gene069681	0.804336	2.27E-49	-1.69E-46
MS.gene057824	MS.gene069859	0.807697	4.45E-50	-1.69E-46
MS.gene057824	MS.gene071062	0.808135	3.59E-50	-1.69E-46
MS.gene057824	MS.gene08264	0.835372	1.74E-56	-1.69E-46
MS.gene057824	MS.gene09574	0.8393	1.72E-57	-1.69E-46
MS.gene057824	MS.gene24098	0.837947	3.85E-57	-1.69E-46
MS.gene057824	MS.gene29105	0.801942	7.10E-49	-1.69E-46
MS.gene057824	MS.gene31035	0.807103	5.95E-50	-1.69E-46
MS.gene057824	MS.gene34874	0.830584	2.70E-55	-1.69E-46
MS.gene057824	MS.gene36952	0.823629	1.25E-53	-1.69E-46
MS.gene057824	MS.gene43589	0.815624	8.41E-52	-1.69E-46
MS.gene057824	MS.gene43919	0.800345	1.51E-48	-1.69E-46
MS.gene057824	MS.gene44640	0.800991	1.11E-48	-1.69E-46
MS.gene057824	MS.gene51389	0.806712	7.20E-50	-1.69E-46
MS.gene057824	MS.gene53143	0.802102	6.58E-49	-1.69E-46
MS.gene057824	MS.gene57193	0.805002	1.65E-49	-1.69E-46
MS.gene057824	MS.gene59149	0.809634	1.72E-50	-1.69E-46
MS.gene057824	MS.gene60558	0.810249	1.27E-50	-1.69E-46
MS.gene057824	MS.gene62822	0.837885	3.99E-57	-1.69E-46
MS.gene057824	MS.gene64994	0.801465	8.89E-49	-1.69E-46
MS.gene057824	MS.gene65683	0.839634	1.41E-57	-1.69E-46
MS.gene057824	MS.gene67422	0.80606	9.88E-50	-1.69E-46
MS.gene057824	MS.gene71481	0.800908	1.16E-48	-1.69E-46
MS.gene057824	MS.gene71664	0.809188	2.14E-50	-1.69E-46
MS.gene057824	MS.gene71836	0.827889	1.22E-54	-1.69E-46
MS.gene057824	MS.gene72747	0.817894	2.60E-52	-1.69E-46
MS.gene057824	MS.gene75176	0.804198	2.42E-49	-1.69E-46
MS.gene057824	MS.gene82533	0.807462	5.00E-50	-1.69E-46
MS.gene057824	MS.gene84472	0.803455	3.46E-49	-1.69E-46
MS.gene057824	MS.gene85860	0.800696	1.28E-48	-1.69E-46
MS.gene057824	MS.gene86081	0.800574	1.35E-48	-1.69E-46
MS.gene057824	MS.gene99874	0.808614	2.84E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057824.t1	MTR_4g076940	100.000	69	0	0	1	69	143	211	3.16e-44	149
MS.gene057824.t1	MTR_4g076940	100.000	69	0	0	1	69	276	344	5.47e-44	149
MS.gene057824.t1	MTR_4g076940	100.000	69	0	0	1	69	236	304	6.19e-44	149
MS.gene057824.t1	MTR_4g076900	95.652	69	3	0	1	69	212	280	2.57e-42	144
MS.gene057824.t1	MTR_4g076970	77.941	68	15	0	1	68	234	301	1.12e-34	123
MS.gene057824.t1	MTR_6g066240	80.882	68	13	0	1	68	249	316	1.14e-33	120
MS.gene057824.t1	MTR_4g094090	69.492	59	18	0	1	59	217	275	2.57e-23	91.3
MS.gene057824.t1	MTR_4g094060	66.102	59	20	0	1	59	259	317	7.95e-22	87.0
MS.gene057824.t1	MTR_4g094010	66.102	59	20	0	1	59	236	294	8.75e-22	87.0
MS.gene057824.t1	MTR_4g093845	65.000	60	21	0	1	60	245	304	1.09e-21	86.7
MS.gene057824.t1	MTR_4g094050	66.102	59	20	0	1	59	259	317	1.53e-21	86.3
MS.gene057824.t1	MTR_4g054020	57.143	70	30	0	1	70	156	225	3.10e-21	85.5
MS.gene057824.t1	MTR_2g095390	54.412	68	31	0	2	69	309	376	1.49e-20	83.6
MS.gene057824.t1	MTR_4g094020	54.167	72	29	1	1	68	169	240	5.38e-17	73.2
MS.gene057824.t1	MTR_4g113070	47.458	59	30	1	1	59	367	424	1.73e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057824.t1	AT3G21090	89.855	69	7	1	69	228	296	4.25e-40	139
MS.gene057824.t1	AT1G51500	91.304	69	6	1	69	228	296	2.55e-39	136
MS.gene057824.t1	AT1G17840	80.882	68	13	1	68	252	319	6.58e-33	118
MS.gene057824.t1	AT1G51460	78.261	69	15	1	69	213	281	9.26e-26	98.6
MS.gene057824.t1	AT2G28070	54.412	68	31	2	69	316	383	1.40e-20	83.6
MS.gene057824.t1	AT2G28070	54.412	68	31	2	69	316	383	1.40e-20	83.6

Find 10 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTCAGAATTGATGCATCTT+AGG	0.314280	4.4:+36300789	None:intergenic
GGTGGCGAAACTGTTTATTT+TGG	0.335273	4.4:-36301173	MS.gene057824:CDS
TTTACGCGGACAAGGGAAAC+CGG	0.462370	4.4:+36300832	None:intergenic
TTCTGACTTTGACGTTGTAA+CGG	0.522158	4.4:-36300774	MS.gene057824:CDS
CGTTGTAACGGCTACACTGA+AGG	0.548225	4.4:-36300762	MS.gene057824:CDS
GTTGTAACGGCTACACTGAA+GGG	0.582706	4.4:-36300761	MS.gene057824:CDS
CATCTTAGGAAGTGATCAGA+AGG	0.588071	4.4:+36300803	None:intergenic
AGTGCAAAGACTTCACTGCT+AGG	0.611217	4.4:+36301221	None:intergenic
GCTACACTGAAGGGATCTCA+AGG	0.644373	4.4:-36300752	MS.gene057824:CDS
TGAAGCTAAAATGGCAATCG+AGG	0.650043	4.4:-36301150	MS.gene057824:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCGAGGTATAAATACATTTT+TGG	-	chr4.4:36300834-36300853	MS.gene057824:CDS	25.0%
!!!	TTATTTTGGTGAAGCTAAAA+TGG	-	chr4.4:36300808-36300827	MS.gene057824:CDS	25.0%
	ATTCATACAATATTGACACG+TGG	-	chr4.4:36301017-36301036	MS.gene057824:intron	30.0%
	AGTCAGAATTGATGCATCTT+AGG	+	chr4.4:36301181-36301200	None:intergenic	35.0%
	ATGTCCTTCTCAAAATGTGA+GGG	+	chr4.4:36300924-36300943	None:intergenic	35.0%
	TTCTGACTTTGACGTTGTAA+CGG	-	chr4.4:36301193-36301212	MS.gene057824:CDS	35.0%
!	TCAGAAGGATTTCTTTTACG+CGG	+	chr4.4:36301152-36301171	None:intergenic	35.0%
	CATCTTAGGAAGTGATCAGA+AGG	+	chr4.4:36301167-36301186	None:intergenic	40.0%
	CATGTCCTTCTCAAAATGTG+AGG	+	chr4.4:36300925-36300944	None:intergenic	40.0%
	TGAAGCTAAAATGGCAATCG+AGG	-	chr4.4:36300817-36300836	MS.gene057824:CDS	40.0%
!	GATGATCTTTTCTTGCTCTC+TGG	-	chr4.4:36300773-36300792	MS.gene057824:CDS	40.0%
!	GATTTCTTTTACGCGGACAA+GGG	+	chr4.4:36301145-36301164	None:intergenic	40.0%
!	GGTGGCGAAACTGTTTATTT+TGG	-	chr4.4:36300794-36300813	MS.gene057824:CDS	40.0%
!!	GATACCCTCACATTTTGAGA+AGG	-	chr4.4:36300917-36300936	MS.gene057824:intron	40.0%
	AGTGCAAAGACTTCACTGCT+AGG	+	chr4.4:36300749-36300768	None:intergenic	45.0%
	GTTGTAACGGCTACACTGAA+GGG	-	chr4.4:36301206-36301225	MS.gene057824:CDS	45.0%
!	GATCTTTTCTTGCTCTCTGG+TGG	-	chr4.4:36300776-36300795	MS.gene057824:CDS	45.0%
!	GGATTTCTTTTACGCGGACA+AGG	+	chr4.4:36301146-36301165	None:intergenic	45.0%
!!!	GTGCAGTTTTTTGCTGAAGC+CGG	-	chr4.4:36301116-36301135	MS.gene057824:intron	45.0%
	CGTTGTAACGGCTACACTGA+AGG	-	chr4.4:36301205-36301224	MS.gene057824:CDS	50.0%
	GCAAAGACTTCACTGCTAGG+AGG	+	chr4.4:36300746-36300765	None:intergenic	50.0%
	GCTACACTGAAGGGATCTCA+AGG	-	chr4.4:36301215-36301234	MS.gene057824:CDS	50.0%
	TTTACGCGGACAAGGGAAAC+CGG	+	chr4.4:36301138-36301157	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	36300746	36301243	36300746	ID=MS.gene057824
chr4.4	mRNA	36300746	36301243	36300746	ID=MS.gene057824.t1;Parent=MS.gene057824
chr4.4	exon	36301151	36301243	36301151	ID=MS.gene057824.t1.exon1;Parent=MS.gene057824.t1
chr4.4	CDS	36301151	36301243	36301151	ID=cds.MS.gene057824.t1;Parent=MS.gene057824.t1
chr4.4	exon	36300746	36300867	36300746	ID=MS.gene057824.t1.exon2;Parent=MS.gene057824.t1
chr4.4	CDS	36300746	36300867	36300746	ID=cds.MS.gene057824.t1;Parent=MS.gene057824.t1

Gene Sequence

>MS.gene057824

CCTAGCAGTGAAGTCTTTGCACTTTTTGATGATCTTTTCTTGCTCTCTGGTGGCGAAACTGTTTATTTTGGTGAAGCTAAAATGGCAATCGAGGTATAAATACATTTTTGGTGTCTGCAAATATTTATAGATTTAAAATGTTTTTAGTCTCTGCAATGATTTTATCTTGTCGATACCCTCACATTTTGAGAAGGACATGTTTTCTCTTATGTAGAAAGACACTTTAAGTTCAAGACGTGTACTGAGTGTCTGAAACATATCAATGTTCGATATTCATACAATATTGACACGTGGATTCACGTGTGTCTGATCATAAATTTTCTCTTCTTCAACAACTAATGCTCATGTTGTATAAATTTTTCGATTTGTTGTGCAGTTTTTTGCTGAAGCCGGTTTCCCTTGTCCGCGTAAAAGAAATCCTTCTGATCACTTCCTAAGATGCATCAATTCTGACTTTGACGTTGTAACGGCTACACTGAAGGGATCTCAAGGATTCCT

Protein sequence

>MS.gene057824.t1

PSSEVFALFDDLFLLSGGETVYFGEAKMAIEFFAEAGFPCPRKRNPSDHFLRCINSDFDVVTATLKGSQGF