Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78382.t1 | XP_003597439.1 | 78.3 | 451 | 92 | 2 | 1 | 451 | 1 | 445 | 3.30E-172 | 614.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78382.t1 | G7IUC5 | 78.3 | 451 | 92 | 2 | 1 | 451 | 1 | 445 | 2.4e-172 | 614.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene78382 | 0.83712 | 6.27E-57 | -1.69E-46 |
| MS.gene049404 | MS.gene78382 | 0.812547 | 4.01E-51 | -1.69E-46 |
| MS.gene049452 | MS.gene78382 | 0.856099 | 4.04E-62 | -1.69E-46 |
| MS.gene049850 | MS.gene78382 | 0.802226 | 6.20E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene78382 | 0.803839 | 2.88E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene78382 | 0.809466 | 1.87E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene78382 | 0.800347 | 1.50E-48 | -1.69E-46 |
| MS.gene050759 | MS.gene78382 | 0.825966 | 3.51E-54 | -1.69E-46 |
| MS.gene050962 | MS.gene78382 | 0.812676 | 3.76E-51 | -1.69E-46 |
| MS.gene051181 | MS.gene78382 | 0.831637 | 1.49E-55 | -1.69E-46 |
| MS.gene051198 | MS.gene78382 | 0.824544 | 7.62E-54 | -1.69E-46 |
| MS.gene051279 | MS.gene78382 | 0.834159 | 3.52E-56 | -1.69E-46 |
| MS.gene051720 | MS.gene78382 | 0.810491 | 1.12E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene78382 | 0.819181 | 1.33E-52 | -1.69E-46 |
| MS.gene051800 | MS.gene78382 | 0.811966 | 5.38E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene78382 | 0.853738 | 1.96E-61 | -1.69E-46 |
| MS.gene052582 | MS.gene78382 | 0.843197 | 1.62E-58 | -1.69E-46 |
| MS.gene052584 | MS.gene78382 | 0.834443 | 2.99E-56 | -1.69E-46 |
| MS.gene052773 | MS.gene78382 | 0.817013 | 4.11E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene78382 | 0.811393 | 7.16E-51 | -1.69E-46 |
| MS.gene052896 | MS.gene78382 | 0.8103 | 1.23E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene78382 | 0.801949 | 7.07E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene78382 | 0.810926 | 9.04E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene78382 | 0.816208 | 6.23E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene78382 | 0.800218 | 1.60E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene78382 | 0.809011 | 2.34E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene78382 | 0.821421 | 4.07E-53 | -1.69E-46 |
| MS.gene05423 | MS.gene78382 | 0.807715 | 4.41E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene78382 | -0.803261 | 3.79E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene78382 | 0.821114 | 4.80E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene78382 | 0.848905 | 4.55E-60 | -1.69E-46 |
| MS.gene054775 | MS.gene78382 | 0.813488 | 2.50E-51 | -1.69E-46 |
| MS.gene055227 | MS.gene78382 | 0.812338 | 4.46E-51 | -1.69E-46 |
| MS.gene055228 | MS.gene78382 | 0.817256 | 3.62E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene78382 | 0.830808 | 2.38E-55 | -1.69E-46 |
| MS.gene055926 | MS.gene78382 | 0.823049 | 1.71E-53 | -1.69E-46 |
| MS.gene056174 | MS.gene78382 | 0.825995 | 3.46E-54 | -1.69E-46 |
| MS.gene056366 | MS.gene78382 | 0.816162 | 6.37E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene78382 | 0.809411 | 1.92E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene78382 | 0.836226 | 1.06E-56 | -1.69E-46 |
| MS.gene057314 | MS.gene78382 | 0.80234 | 5.88E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene78382 | 0.803974 | 2.70E-49 | -1.69E-46 |
| MS.gene058009 | MS.gene78382 | 0.810937 | 8.99E-51 | -1.69E-46 |
| MS.gene058010 | MS.gene78382 | 0.821422 | 4.07E-53 | -1.69E-46 |
| MS.gene058037 | MS.gene78382 | 0.833929 | 4.02E-56 | -1.69E-46 |
| MS.gene058625 | MS.gene78382 | 0.812718 | 3.68E-51 | -1.69E-46 |
| MS.gene058783 | MS.gene78382 | 0.808239 | 3.41E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene78382 | 0.832646 | 8.39E-56 | -1.69E-46 |
| MS.gene059584 | MS.gene78382 | 0.803317 | 3.69E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene78382 | 0.811972 | 5.36E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene78382 | 0.813917 | 2.01E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene78382 | 0.812166 | 4.86E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene78382 | 0.815632 | 8.37E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene78382 | 0.814869 | 1.24E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78382.t1 | MTR_2g098080 | 94.966 | 437 | 17 | 1 | 15 | 451 | 14 | 445 | 0.0 | 838 |
| MS.gene78382.t1 | MTR_1g098920 | 47.500 | 360 | 162 | 8 | 88 | 422 | 92 | 449 | 5.32e-95 | 294 |
| MS.gene78382.t1 | MTR_6g035315 | 46.450 | 338 | 154 | 4 | 106 | 417 | 105 | 441 | 5.86e-82 | 262 |
| MS.gene78382.t1 | MTR_7g103390 | 44.531 | 256 | 100 | 6 | 4 | 246 | 3 | 229 | 3.08e-58 | 198 |
| MS.gene78382.t1 | MTR_1g061640 | 59.627 | 161 | 59 | 3 | 87 | 246 | 94 | 249 | 1.22e-54 | 189 |
| MS.gene78382.t1 | MTR_1g061640 | 41.441 | 111 | 61 | 1 | 313 | 419 | 371 | 481 | 3.52e-23 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78382.t1 | AT1G21200 | 41.149 | 435 | 187 | 10 | 27 | 422 | 39 | 443 | 1.07e-87 | 275 |
| MS.gene78382.t1 | AT1G21200 | 41.149 | 435 | 187 | 10 | 27 | 422 | 39 | 443 | 1.07e-87 | 275 |
| MS.gene78382.t1 | AT1G76870 | 42.651 | 347 | 167 | 10 | 87 | 422 | 60 | 385 | 2.43e-71 | 230 |
| MS.gene78382.t1 | AT1G76870 | 42.651 | 347 | 167 | 10 | 87 | 422 | 60 | 385 | 2.43e-71 | 230 |
| MS.gene78382.t1 | AT1G76870 | 42.651 | 347 | 167 | 10 | 87 | 422 | 60 | 385 | 2.43e-71 | 230 |
| MS.gene78382.t1 | AT3G10040 | 37.366 | 372 | 189 | 6 | 89 | 433 | 74 | 428 | 3.41e-66 | 218 |
Find 115 sgRNAs with CRISPR-Local
Find 137 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAATAATAATAATCAAAT+TGG | 0.228579 | 2.3:+5190831 | MS.gene78382:CDS |
| TTCTCCCACGGTGACTTTGC+AGG | 0.254875 | 2.3:-5191695 | None:intergenic |
| TCAATTATGAATGGTTCTTC+TGG | 0.281471 | 2.3:+5190666 | None:intergenic |
| TGCCTATGAAATGGAGATTC+AGG | 0.292024 | 2.3:-5190735 | None:intergenic |
| GGTTCTTCTGGTTTGGGTGG+TGG | 0.300179 | 2.3:+5190678 | MS.gene78382:CDS |
| TATGAATGGTTCTTCTGGTT+TGG | 0.302937 | 2.3:+5190671 | None:intergenic |
| CAAGTCAAGATCATGACTAT+TGG | 0.311830 | 2.3:-5191423 | None:intergenic |
| ATGAATGGTTCTTCTGGTTT+GGG | 0.316755 | 2.3:+5190672 | MS.gene78382:CDS |
| GTTCTTCTGGTTTGGGTGGT+GGG | 0.328898 | 2.3:+5190679 | MS.gene78382:CDS |
| ACCAATGGAGGTAGGGTCTA+AGG | 0.337698 | 2.3:-5191954 | None:intergenic |
| GTGAGGAGGATGGCCACTTT+TGG | 0.369260 | 2.3:+5191615 | MS.gene78382:CDS |
| GGAGAAAAGAGAGTGGATTA+AGG | 0.381772 | 2.3:+5191712 | MS.gene78382:CDS |
| TATGAATATGATAACTGGTT+TGG | 0.383314 | 2.3:+5190794 | MS.gene78382:CDS |
| GGATAGGGAGCTGGAGAAGC+TGG | 0.386271 | 2.3:+5191835 | MS.gene78382:CDS |
| CAACATATGAATATGATAAC+TGG | 0.387205 | 2.3:+5190789 | MS.gene78382:CDS |
| TCCTGCATTGATGGATTCAA+TGG | 0.411710 | 2.3:+5191298 | MS.gene78382:CDS |
| GAGGATGATGAGTTGGATAA+TGG | 0.423042 | 2.3:+5191527 | MS.gene78382:CDS |
| AAGTCAAGATCATGACTATT+GGG | 0.423237 | 2.3:-5191422 | None:intergenic |
| GAAGTTCAAAAGCCCTGGCT+TGG | 0.432989 | 2.3:-5191772 | None:intergenic |
| GAGCATGGTTACGGAGAAGA+TGG | 0.435266 | 2.3:+5190945 | MS.gene78382:CDS |
| TCTCCCACGGTGACTTTGCA+GGG | 0.439902 | 2.3:-5191694 | None:intergenic |
| AATCGTGTTTCAATTATGAA+TGG | 0.449194 | 2.3:+5190657 | None:intergenic |
| TGGTAGGGGAACTTGTTGTC+AGG | 0.449451 | 2.3:+5191265 | MS.gene78382:CDS |
| GAAGACGATGAGCATGGTTA+CGG | 0.454149 | 2.3:+5190936 | MS.gene78382:CDS |
| ACCCTGAATCTCCATTTCAT+AGG | 0.455146 | 2.3:+5190733 | MS.gene78382:CDS |
| GTGAGTTGTGTTGGTGATGA+TGG | 0.455421 | 2.3:+5191068 | MS.gene78382:CDS |
| GAAGAAACTTGAGAGTGAAA+TGG | 0.458951 | 2.3:+5191655 | MS.gene78382:CDS |
| GGTTGAGAATCCTGCATTGA+TGG | 0.463782 | 2.3:+5191289 | MS.gene78382:CDS |
| GATACCGCAGCAAGAAGGAT+AGG | 0.473004 | 2.3:+5191819 | MS.gene78382:CDS |
| ATCCAGGGTGTCACAGAAGA+AGG | 0.479228 | 2.3:+5191124 | MS.gene78382:CDS |
| TGACTTGCATAGTTATTCGT+TGG | 0.479428 | 2.3:+5191439 | MS.gene78382:CDS |
| TTCAGAAACAGCGGTTTAAG+TGG | 0.489001 | 2.3:+5191792 | MS.gene78382:CDS |
| ACCATTGAATCCATCAATGC+AGG | 0.492133 | 2.3:-5191299 | None:intergenic |
| AATATTAATGCACGTGGTGA+TGG | 0.497668 | 2.3:+5191554 | MS.gene78382:CDS |
| GGTGATGGAGGGAGAATGGA+AGG | 0.501886 | 2.3:+5191569 | MS.gene78382:CDS |
| TCTAAGATAATGATAAGTAA+AGG | 0.502250 | 2.3:+5191164 | MS.gene78382:CDS |
| AATGGTTAACTTGTCTGCTA+AGG | 0.514836 | 2.3:+5191316 | MS.gene78382:CDS |
| TTGTGGCCTCTTGATACCAA+TGG | 0.514883 | 2.3:-5191969 | None:intergenic |
| AATGGTTCTTCTGGTTTGGG+TGG | 0.516998 | 2.3:+5190675 | MS.gene78382:CDS |
| TCCTTAGACCCTACCTCCAT+TGG | 0.518261 | 2.3:+5191953 | MS.gene78382:CDS |
| GGTTGAATGAGATGCTTGGT+AGG | 0.519981 | 2.3:+5191249 | MS.gene78382:CDS |
| TGTTGTTGATGCCTATGAAA+TGG | 0.522650 | 2.3:-5190744 | None:intergenic |
| CATAGTTATTCGTTGGAACA+CGG | 0.524019 | 2.3:+5191446 | MS.gene78382:CDS |
| ATGGTGTTAAGAAGAAATCC+AGG | 0.526175 | 2.3:+5191108 | MS.gene78382:CDS |
| AAAATGTTGACTTCCAAGCC+AGG | 0.526284 | 2.3:+5191759 | MS.gene78382:CDS |
| CACTAGACTGCGGCCAAAAG+TGG | 0.527780 | 2.3:-5191628 | None:intergenic |
| TTTCTGAAGTTCAAAAGCCC+TGG | 0.531742 | 2.3:-5191777 | None:intergenic |
| GGTGATGATGGAACAATTAG+TGG | 0.532799 | 2.3:+5191080 | MS.gene78382:CDS |
| ACCCTTCTTCTGTGACACCC+TGG | 0.535802 | 2.3:-5191126 | None:intergenic |
| CAGCAAGAAGGATAGGGAGC+TGG | 0.539921 | 2.3:+5191826 | MS.gene78382:CDS |
| TGGGTATCCTTTGACCATTG+TGG | 0.541818 | 2.3:-5191403 | None:intergenic |
| ATTAATGCACGTGGTGATGG+AGG | 0.542789 | 2.3:+5191557 | MS.gene78382:CDS |
| TAACTTGTCTGCTAAGGCTA+AGG | 0.548373 | 2.3:+5191322 | MS.gene78382:CDS |
| TGAAGATAACAATGAAAGTG+AGG | 0.549731 | 2.3:+5191508 | MS.gene78382:CDS |
| ATAGCAGTGGTGAGTTGTGT+TGG | 0.551265 | 2.3:+5191059 | MS.gene78382:CDS |
| GCCTATGAAATGGAGATTCA+GGG | 0.555779 | 2.3:-5190734 | None:intergenic |
| TAGGGGAACTTGTTGTCAGG+TGG | 0.556404 | 2.3:+5191268 | MS.gene78382:CDS |
| TGAAAGTGAGGATGATGAGT+TGG | 0.557152 | 2.3:+5191520 | MS.gene78382:CDS |
| AACTTGAGAGTGAAATGGCA+AGG | 0.557312 | 2.3:+5191660 | MS.gene78382:CDS |
| GTTGAATGAGATGCTTGGTA+GGG | 0.559093 | 2.3:+5191250 | MS.gene78382:CDS |
| TATCAAGAGGCCACAAGGGA+GGG | 0.560647 | 2.3:+5191976 | MS.gene78382:CDS |
| ATACCGCAGCAAGAAGGATA+GGG | 0.564239 | 2.3:+5191820 | MS.gene78382:CDS |
| AAAGCTGAAGCAGAGAGAGC+AGG | 0.565355 | 2.3:+5191904 | MS.gene78382:CDS |
| TTGATATGTTCCCTCCCTTG+TGG | 0.565958 | 2.3:-5191986 | None:intergenic |
| GTCCACTTCATTCTCTGCCA+AGG | 0.566514 | 2.3:-5191014 | None:intergenic |
| ACTTGAATAAGAGATATAAG+AGG | 0.568508 | 2.3:+5191228 | MS.gene78382:CDS |
| TGTCACAGAAGAAGGGTAAG+TGG | 0.569426 | 2.3:+5191132 | MS.gene78382:CDS |
| TCTTGATACCAATGGAGGTA+GGG | 0.573392 | 2.3:-5191961 | None:intergenic |
| GGAGCTGGAGAAGCTGGCGA+TGG | 0.574129 | 2.3:+5191841 | MS.gene78382:CDS |
| AAGAGGTTGAATGAGATGCT+TGG | 0.576189 | 2.3:+5191245 | MS.gene78382:CDS |
| AGCAGGAGGCAGAGTTCAGT+AGG | 0.578594 | 2.3:+5191921 | MS.gene78382:CDS |
| CAACAGACTCAGTTAGCTCA+TGG | 0.578956 | 2.3:+5190762 | MS.gene78382:CDS |
| CTCTTGATACCAATGGAGGT+AGG | 0.581041 | 2.3:-5191962 | None:intergenic |
| TTGAATGAGATGCTTGGTAG+GGG | 0.587060 | 2.3:+5191251 | MS.gene78382:CDS |
| TAGAAATAAATTAAGTGAGG+AGG | 0.587853 | 2.3:+5191601 | MS.gene78382:CDS |
| ACGTGGTGATGGAGGGAGAA+TGG | 0.588300 | 2.3:+5191565 | MS.gene78382:CDS |
| TGATAGAAATAAATTAAGTG+AGG | 0.588915 | 2.3:+5191598 | MS.gene78382:CDS |
| GTTAAGATACCGCAGCAAGA+AGG | 0.589509 | 2.3:+5191814 | MS.gene78382:CDS |
| TTAATGCACGTGGTGATGGA+GGG | 0.592885 | 2.3:+5191558 | MS.gene78382:CDS |
| AAATGTTGACTTCCAAGCCA+GGG | 0.593930 | 2.3:+5191760 | MS.gene78382:CDS |
| ATAGTTATTCGTTGGAACAC+GGG | 0.600371 | 2.3:+5191447 | MS.gene78382:CDS |
| TTATTCGTTGGAACACGGGA+AGG | 0.601197 | 2.3:+5191451 | MS.gene78382:CDS |
| GTGCATACCACAATGGTCAA+AGG | 0.603169 | 2.3:+5191396 | MS.gene78382:CDS |
| TGATGGAACAATTAGTGGTG+TGG | 0.604354 | 2.3:+5191085 | MS.gene78382:CDS |
| ACTTGAGAGTGAAATGGCAA+GGG | 0.606060 | 2.3:+5191661 | MS.gene78382:CDS |
| TCCAGGGTGTCACAGAAGAA+GGG | 0.606142 | 2.3:+5191125 | MS.gene78382:CDS |
| AGTGGACGGATAATGTGGTT+GGG | 0.610749 | 2.3:+5191030 | MS.gene78382:CDS |
| AAGTGGACGGATAATGTGGT+TGG | 0.613391 | 2.3:+5191029 | MS.gene78382:CDS |
| GTATCAAGAGGCCACAAGGG+AGG | 0.615865 | 2.3:+5191975 | MS.gene78382:CDS |
| AACTTATCCTCACACTGCTG+CGG | 0.618536 | 2.3:-5191200 | None:intergenic |
| CTACCTCCATTGGTATCAAG+AGG | 0.620308 | 2.3:+5191963 | MS.gene78382:CDS |
| AGCATGGTTACGGAGAAGAT+GGG | 0.623013 | 2.3:+5190946 | MS.gene78382:CDS |
| GGAACAATTAGTGGTGTGGA+TGG | 0.625621 | 2.3:+5191089 | MS.gene78382:CDS |
| AATAAATTAAGTGAGGAGGA+TGG | 0.626359 | 2.3:+5191605 | MS.gene78382:CDS |
| AATGAAGTGGACGGATAATG+TGG | 0.631463 | 2.3:+5191025 | MS.gene78382:CDS |
| AGGGAGGGAACATATCAACT+TGG | 0.631664 | 2.3:+5191991 | MS.gene78382:CDS |
| CTCCTTGGCAGAGAATGAAG+TGG | 0.631774 | 2.3:+5191012 | MS.gene78382:CDS |
| TGTGTCGCCGCAGCAGTGTG+AGG | 0.632288 | 2.3:+5191193 | MS.gene78382:CDS |
| GCTCCCTATCCTTCTTGCTG+CGG | 0.632768 | 2.3:-5191823 | None:intergenic |
| GGTTGGGCTTCTTATAGCAG+TGG | 0.632989 | 2.3:+5191046 | MS.gene78382:CDS |
| ATTGGTATCAAGAGGCCACA+AGG | 0.633070 | 2.3:+5191971 | MS.gene78382:CDS |
| TTCTTCATCCCACTAGACTG+CGG | 0.642211 | 2.3:-5191638 | None:intergenic |
| GCTGAAGCAGAGAGAGCAGG+AGG | 0.642379 | 2.3:+5191907 | MS.gene78382:CDS |
| ATGAGCGAAGACGATGAGCA+TGG | 0.644222 | 2.3:+5190930 | MS.gene78382:CDS |
| TTGGCAGAGAATGAAGTGGA+CGG | 0.645694 | 2.3:+5191016 | MS.gene78382:CDS |
| CACCGTGGGAGAAAAGAGAG+TGG | 0.647688 | 2.3:+5191705 | MS.gene78382:CDS |
| TGGCCTCTTGATACCAATGG+AGG | 0.649145 | 2.3:-5191966 | None:intergenic |
| GTGATGGAGGGAGAATGGAA+GGG | 0.653559 | 2.3:+5191570 | MS.gene78382:CDS |
| AGACCCTGCAAAGTCACCGT+GGG | 0.657508 | 2.3:+5191691 | MS.gene78382:CDS |
| TTGGTATCAAGAGGCCACAA+GGG | 0.674612 | 2.3:+5191972 | MS.gene78382:CDS |
| GAAATGTGTGCATACCACAA+TGG | 0.676978 | 2.3:+5191389 | MS.gene78382:CDS |
| GGAATTAATATTAATGCACG+TGG | 0.680730 | 2.3:+5191548 | MS.gene78382:CDS |
| TGGTGTTAAGAAGAAATCCA+GGG | 0.687571 | 2.3:+5191109 | MS.gene78382:CDS |
| AAGACCCTGCAAAGTCACCG+TGG | 0.699063 | 2.3:+5191690 | MS.gene78382:CDS |
| AAGGACTCAAGAGATCACGA+TGG | 0.786747 | 2.3:+5191470 | MS.gene78382:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAATTTGAATTTTGGTAA+AGG | + | chr2.3:5190867-5190886 | MS.gene78382:CDS | 15.0% |
| !! | TGATAGAAATAAATTAAGTG+AGG | + | chr2.3:5191598-5191617 | MS.gene78382:CDS | 20.0% |
| !!! | GAAGTGAAAAATTTGAATTT+TGG | + | chr2.3:5190861-5190880 | MS.gene78382:CDS | 20.0% |
| !!! | TCTAAGATAATGATAAGTAA+AGG | + | chr2.3:5191164-5191183 | MS.gene78382:CDS | 20.0% |
| ! | ACTTGAATAAGAGATATAAG+AGG | + | chr2.3:5191228-5191247 | MS.gene78382:CDS | 25.0% |
| ! | CAACATATGAATATGATAAC+TGG | + | chr2.3:5190789-5190808 | MS.gene78382:CDS | 25.0% |
| ! | TAGAAATAAATTAAGTGAGG+AGG | + | chr2.3:5191601-5191620 | MS.gene78382:CDS | 25.0% |
| ! | TATGAATATGATAACTGGTT+TGG | + | chr2.3:5190794-5190813 | MS.gene78382:CDS | 25.0% |
| !!! | TTGAATTTTGGTAAAGGAAA+GGG | + | chr2.3:5190873-5190892 | MS.gene78382:CDS | 25.0% |
| !!! | TTTGAATTTTGGTAAAGGAA+AGG | + | chr2.3:5190872-5190891 | MS.gene78382:CDS | 25.0% |
| AAGTCAAGATCATGACTATT+GGG | - | chr2.3:5191425-5191444 | None:intergenic | 30.0% | |
| AATAAATTAAGTGAGGAGGA+TGG | + | chr2.3:5191605-5191624 | MS.gene78382:CDS | 30.0% | |
| GGAATTAATATTAATGCACG+TGG | + | chr2.3:5191548-5191567 | MS.gene78382:CDS | 30.0% | |
| TGAAGATAACAATGAAAGTG+AGG | + | chr2.3:5191508-5191527 | MS.gene78382:CDS | 30.0% | |
| !!! | AATTGCGAGAATTTTTTCGA+TGG | + | chr2.3:5190969-5190988 | MS.gene78382:CDS | 30.0% |
| AATATTAATGCACGTGGTGA+TGG | + | chr2.3:5191554-5191573 | MS.gene78382:CDS | 35.0% | |
| ATAGTTATTCGTTGGAACAC+GGG | + | chr2.3:5191447-5191466 | MS.gene78382:CDS | 35.0% | |
| CAAGTCAAGATCATGACTAT+TGG | - | chr2.3:5191426-5191445 | None:intergenic | 35.0% | |
| CATAGTTATTCGTTGGAACA+CGG | + | chr2.3:5191446-5191465 | MS.gene78382:CDS | 35.0% | |
| GAAGAAACTTGAGAGTGAAA+TGG | + | chr2.3:5191655-5191674 | MS.gene78382:CDS | 35.0% | |
| TGTTGTTGATGCCTATGAAA+TGG | - | chr2.3:5190747-5190766 | None:intergenic | 35.0% | |
| ! | ATGGTGTTAAGAAGAAATCC+AGG | + | chr2.3:5191108-5191127 | MS.gene78382:CDS | 35.0% |
| ! | CTTTTGAACTTCAGAAACAG+CGG | + | chr2.3:5191783-5191802 | MS.gene78382:CDS | 35.0% |
| ! | TGACTTGCATAGTTATTCGT+TGG | + | chr2.3:5191439-5191458 | MS.gene78382:CDS | 35.0% |
| ! | TGGTGTTAAGAAGAAATCCA+GGG | + | chr2.3:5191109-5191128 | MS.gene78382:CDS | 35.0% |
| !! | AATGGTTAACTTGTCTGCTA+AGG | + | chr2.3:5191316-5191335 | MS.gene78382:CDS | 35.0% |
| !! | ATGAATGGTTCTTCTGGTTT+GGG | + | chr2.3:5190672-5190691 | MS.gene78382:CDS | 35.0% |
| !!! | AATTTTTTCGATGGTGGGAA+AGG | + | chr2.3:5190978-5190997 | MS.gene78382:CDS | 35.0% |
| !!! | ATTTTTTCGATGGTGGGAAA+GGG | + | chr2.3:5190979-5190998 | MS.gene78382:CDS | 35.0% |
| AACTTGAGAGTGAAATGGCA+AGG | + | chr2.3:5191660-5191679 | MS.gene78382:CDS | 40.0% | |
| AAGAGGTTGAATGAGATGCT+TGG | + | chr2.3:5191245-5191264 | MS.gene78382:CDS | 40.0% | |
| AATGAAGTGGACGGATAATG+TGG | + | chr2.3:5191025-5191044 | MS.gene78382:CDS | 40.0% | |
| ACCATTGAATCCATCAATGC+AGG | - | chr2.3:5191302-5191321 | None:intergenic | 40.0% | |
| ACCCTGAATCTCCATTTCAT+AGG | + | chr2.3:5190733-5190752 | MS.gene78382:CDS | 40.0% | |
| ACTTGAGAGTGAAATGGCAA+GGG | + | chr2.3:5191661-5191680 | MS.gene78382:CDS | 40.0% | |
| GAAATGTGTGCATACCACAA+TGG | + | chr2.3:5191389-5191408 | MS.gene78382:CDS | 40.0% | |
| GCCTATGAAATGGAGATTCA+GGG | - | chr2.3:5190737-5190756 | None:intergenic | 40.0% | |
| GTTGAATGAGATGCTTGGTA+GGG | + | chr2.3:5191250-5191269 | MS.gene78382:CDS | 40.0% | |
| TCTTGATACCAATGGAGGTA+GGG | - | chr2.3:5191964-5191983 | None:intergenic | 40.0% | |
| TGAAAGTGAGGATGATGAGT+TGG | + | chr2.3:5191520-5191539 | MS.gene78382:CDS | 40.0% | |
| TGATGGAACAATTAGTGGTG+TGG | + | chr2.3:5191085-5191104 | MS.gene78382:CDS | 40.0% | |
| TGCCTATGAAATGGAGATTC+AGG | - | chr2.3:5190738-5190757 | None:intergenic | 40.0% | |
| TTCAGAAACAGCGGTTTAAG+TGG | + | chr2.3:5191792-5191811 | MS.gene78382:CDS | 40.0% | |
| TTGAATGAGATGCTTGGTAG+GGG | + | chr2.3:5191251-5191270 | MS.gene78382:CDS | 40.0% | |
| ! | ATGGTGGGAAAGGGAAAAAA+GGG | + | chr2.3:5190988-5191007 | MS.gene78382:CDS | 40.0% |
| ! | GAGGATGATGAGTTGGATAA+TGG | + | chr2.3:5191527-5191546 | MS.gene78382:CDS | 40.0% |
| ! | GGAAGTCAACATTTTGCTCT+TGG | - | chr2.3:5191754-5191773 | None:intergenic | 40.0% |
| ! | TAACTTGTCTGCTAAGGCTA+AGG | + | chr2.3:5191322-5191341 | MS.gene78382:CDS | 40.0% |
| !! | AAAATGTTGACTTCCAAGCC+AGG | + | chr2.3:5191759-5191778 | MS.gene78382:CDS | 40.0% |
| !! | AAATGTTGACTTCCAAGCCA+GGG | + | chr2.3:5191760-5191779 | MS.gene78382:CDS | 40.0% |
| !! | GGAGAAAAGAGAGTGGATTA+AGG | + | chr2.3:5191712-5191731 | MS.gene78382:CDS | 40.0% |
| !! | GGTGATGATGGAACAATTAG+TGG | + | chr2.3:5191080-5191099 | MS.gene78382:CDS | 40.0% |
| !! | TCCTGCATTGATGGATTCAA+TGG | + | chr2.3:5191298-5191317 | MS.gene78382:CDS | 40.0% |
| !! | TTTCTGAAGTTCAAAAGCCC+TGG | - | chr2.3:5191780-5191799 | None:intergenic | 40.0% |
| !!! | GCGAGAATTTTTTCGATGGT+GGG | + | chr2.3:5190973-5190992 | MS.gene78382:CDS | 40.0% |
| !!! | TGCGAGAATTTTTTCGATGG+TGG | + | chr2.3:5190972-5190991 | MS.gene78382:CDS | 40.0% |
| AACTTATCCTCACACTGCTG+CGG | - | chr2.3:5191203-5191222 | None:intergenic | 45.0% | |
| AAGGACTCAAGAGATCACGA+TGG | + | chr2.3:5191470-5191489 | MS.gene78382:CDS | 45.0% | |
| AAGGGAAAAAAGGGTCTCCT+TGG | + | chr2.3:5190997-5191016 | MS.gene78382:CDS | 45.0% | |
| AAGTGGACGGATAATGTGGT+TGG | + | chr2.3:5191029-5191048 | MS.gene78382:CDS | 45.0% | |
| AGCATGGTTACGGAGAAGAT+GGG | + | chr2.3:5190946-5190965 | MS.gene78382:CDS | 45.0% | |
| AGGGAGGGAACATATCAACT+TGG | + | chr2.3:5191991-5192010 | MS.gene78382:CDS | 45.0% | |
| AGTGGACGGATAATGTGGTT+GGG | + | chr2.3:5191030-5191049 | MS.gene78382:CDS | 45.0% | |
| ATACCGCAGCAAGAAGGATA+GGG | + | chr2.3:5191820-5191839 | MS.gene78382:CDS | 45.0% | |
| ATAGCAGTGGTGAGTTGTGT+TGG | + | chr2.3:5191059-5191078 | MS.gene78382:CDS | 45.0% | |
| ATTGGTATCAAGAGGCCACA+AGG | + | chr2.3:5191971-5191990 | MS.gene78382:CDS | 45.0% | |
| CAACAGACTCAGTTAGCTCA+TGG | + | chr2.3:5190762-5190781 | MS.gene78382:CDS | 45.0% | |
| CTACCTCCATTGGTATCAAG+AGG | + | chr2.3:5191963-5191982 | MS.gene78382:CDS | 45.0% | |
| CTCTTGATACCAATGGAGGT+AGG | - | chr2.3:5191965-5191984 | None:intergenic | 45.0% | |
| GAAGACGATGAGCATGGTTA+CGG | + | chr2.3:5190936-5190955 | MS.gene78382:CDS | 45.0% | |
| GGAACAATTAGTGGTGTGGA+TGG | + | chr2.3:5191089-5191108 | MS.gene78382:CDS | 45.0% | |
| GGTTGAATGAGATGCTTGGT+AGG | + | chr2.3:5191249-5191268 | MS.gene78382:CDS | 45.0% | |
| GGTTGAGAATCCTGCATTGA+TGG | + | chr2.3:5191289-5191308 | MS.gene78382:CDS | 45.0% | |
| GTGCATACCACAATGGTCAA+AGG | + | chr2.3:5191396-5191415 | MS.gene78382:CDS | 45.0% | |
| GTTAAGATACCGCAGCAAGA+AGG | + | chr2.3:5191814-5191833 | MS.gene78382:CDS | 45.0% | |
| TGGGTATCCTTTGACCATTG+TGG | - | chr2.3:5191406-5191425 | None:intergenic | 45.0% | |
| TGTCACAGAAGAAGGGTAAG+TGG | + | chr2.3:5191132-5191151 | MS.gene78382:CDS | 45.0% | |
| TTATTCGTTGGAACACGGGA+AGG | + | chr2.3:5191451-5191470 | MS.gene78382:CDS | 45.0% | |
| TTCTTCATCCCACTAGACTG+CGG | - | chr2.3:5191641-5191660 | None:intergenic | 45.0% | |
| TTGATATGTTCCCTCCCTTG+TGG | - | chr2.3:5191989-5192008 | None:intergenic | 45.0% | |
| TTGGCAGAGAATGAAGTGGA+CGG | + | chr2.3:5191016-5191035 | MS.gene78382:CDS | 45.0% | |
| TTGGTATCAAGAGGCCACAA+GGG | + | chr2.3:5191972-5191991 | MS.gene78382:CDS | 45.0% | |
| TTGTGGCCTCTTGATACCAA+TGG | - | chr2.3:5191972-5191991 | None:intergenic | 45.0% | |
| ! | ATCCACTCTCTTTTCTCCCA+CGG | - | chr2.3:5191710-5191729 | None:intergenic | 45.0% |
| !! | AATGGTTCTTCTGGTTTGGG+TGG | + | chr2.3:5190675-5190694 | MS.gene78382:CDS | 45.0% |
| !! | ATTAATGCACGTGGTGATGG+AGG | + | chr2.3:5191557-5191576 | MS.gene78382:CDS | 45.0% |
| !! | GATGGTGGGAAAGGGAAAAA+AGG | + | chr2.3:5190987-5191006 | MS.gene78382:CDS | 45.0% |
| !! | GTGAGTTGTGTTGGTGATGA+TGG | + | chr2.3:5191068-5191087 | MS.gene78382:CDS | 45.0% |
| !! | TTAATGCACGTGGTGATGGA+GGG | + | chr2.3:5191558-5191577 | MS.gene78382:CDS | 45.0% |
| !! | AATAATAATAATAATCAAAT+TGG | + | chr2.3:5190831-5190850 | MS.gene78382:CDS | 5.0% |
| AAAGCTGAAGCAGAGAGAGC+AGG | + | chr2.3:5191904-5191923 | MS.gene78382:CDS | 50.0% | |
| ACCAATGGAGGTAGGGTCTA+AGG | - | chr2.3:5191957-5191976 | None:intergenic | 50.0% | |
| ATGAGCGAAGACGATGAGCA+TGG | + | chr2.3:5190930-5190949 | MS.gene78382:CDS | 50.0% | |
| CTCCTTGGCAGAGAATGAAG+TGG | + | chr2.3:5191012-5191031 | MS.gene78382:CDS | 50.0% | |
| GAAGTTCAAAAGCCCTGGCT+TGG | - | chr2.3:5191775-5191794 | None:intergenic | 50.0% | |
| GAGCATGGTTACGGAGAAGA+TGG | + | chr2.3:5190945-5190964 | MS.gene78382:CDS | 50.0% | |
| GATACCGCAGCAAGAAGGAT+AGG | + | chr2.3:5191819-5191838 | MS.gene78382:CDS | 50.0% | |
| GGTTGGGCTTCTTATAGCAG+TGG | + | chr2.3:5191046-5191065 | MS.gene78382:CDS | 50.0% | |
| GTCCACTTCATTCTCTGCCA+AGG | - | chr2.3:5191017-5191036 | None:intergenic | 50.0% | |
| TATCAAGAGGCCACAAGGGA+GGG | + | chr2.3:5191976-5191995 | MS.gene78382:CDS | 50.0% | |
| TCCTTAGACCCTACCTCCAT+TGG | + | chr2.3:5191953-5191972 | MS.gene78382:CDS | 50.0% | |
| TGGCCTCTTGATACCAATGG+AGG | - | chr2.3:5191969-5191988 | None:intergenic | 50.0% | |
| ! | ACTTTTGGCCGCAGTCTAGT+GGG | + | chr2.3:5191630-5191649 | MS.gene78382:CDS | 50.0% |
| ! | ATCCAGGGTGTCACAGAAGA+AGG | + | chr2.3:5191124-5191143 | MS.gene78382:CDS | 50.0% |
| ! | GTGATGGAGGGAGAATGGAA+GGG | + | chr2.3:5191570-5191589 | MS.gene78382:CDS | 50.0% |
| ! | TCCAGGGTGTCACAGAAGAA+GGG | + | chr2.3:5191125-5191144 | MS.gene78382:CDS | 50.0% |
| !! | GTTCTTCTGGTTTGGGTGGT+GGG | + | chr2.3:5190679-5190698 | MS.gene78382:CDS | 50.0% |
| !! | TAGGGGAACTTGTTGTCAGG+TGG | + | chr2.3:5191268-5191287 | MS.gene78382:CDS | 50.0% |
| !! | TGGTAGGGGAACTTGTTGTC+AGG | + | chr2.3:5191265-5191284 | MS.gene78382:CDS | 50.0% |
| !!! | GGTTTTTGAGTGGTCCCACT+GGG | + | chr2.3:5190700-5190719 | MS.gene78382:CDS | 50.0% |
| !!! | GTTTTTGAGTGGTCCCACTG+GGG | + | chr2.3:5190701-5190720 | MS.gene78382:CDS | 50.0% |
| !!! | TTGGGTGGTGGGTTTTTGAG+TGG | + | chr2.3:5190690-5190709 | MS.gene78382:CDS | 50.0% |
| !!! | TTTTTGAGTGGTCCCACTGG+GGG | + | chr2.3:5190702-5190721 | MS.gene78382:CDS | 50.0% |
| AAGACCCTGCAAAGTCACCG+TGG | + | chr2.3:5191690-5191709 | MS.gene78382:CDS | 55.0% | |
| ACCCTTCTTCTGTGACACCC+TGG | - | chr2.3:5191129-5191148 | None:intergenic | 55.0% | |
| AGACCCTGCAAAGTCACCGT+GGG | + | chr2.3:5191691-5191710 | MS.gene78382:CDS | 55.0% | |
| AGCAGGAGGCAGAGTTCAGT+AGG | + | chr2.3:5191921-5191940 | MS.gene78382:CDS | 55.0% | |
| AGGGTCCAAAATACCCCCAG+TGG | - | chr2.3:5190718-5190737 | None:intergenic | 55.0% | |
| CACCGTGGGAGAAAAGAGAG+TGG | + | chr2.3:5191705-5191724 | MS.gene78382:CDS | 55.0% | |
| CACTAGACTGCGGCCAAAAG+TGG | - | chr2.3:5191631-5191650 | None:intergenic | 55.0% | |
| CAGCAAGAAGGATAGGGAGC+TGG | + | chr2.3:5191826-5191845 | MS.gene78382:CDS | 55.0% | |
| GCTCCCTATCCTTCTTGCTG+CGG | - | chr2.3:5191826-5191845 | None:intergenic | 55.0% | |
| GGGTCCAAAATACCCCCAGT+GGG | - | chr2.3:5190717-5190736 | None:intergenic | 55.0% | |
| GTATCAAGAGGCCACAAGGG+AGG | + | chr2.3:5191975-5191994 | MS.gene78382:CDS | 55.0% | |
| TCTCCCACGGTGACTTTGCA+GGG | - | chr2.3:5191697-5191716 | None:intergenic | 55.0% | |
| TTCTCCCACGGTGACTTTGC+AGG | - | chr2.3:5191698-5191717 | None:intergenic | 55.0% | |
| ! | CACTTTTGGCCGCAGTCTAG+TGG | + | chr2.3:5191629-5191648 | MS.gene78382:CDS | 55.0% |
| !! | ACGTGGTGATGGAGGGAGAA+TGG | + | chr2.3:5191565-5191584 | MS.gene78382:CDS | 55.0% |
| !! | GGTGATGGAGGGAGAATGGA+AGG | + | chr2.3:5191569-5191588 | MS.gene78382:CDS | 55.0% |
| !! | GGTTCTTCTGGTTTGGGTGG+TGG | + | chr2.3:5190678-5190697 | MS.gene78382:CDS | 55.0% |
| !! | GTGAGGAGGATGGCCACTTT+TGG | + | chr2.3:5191615-5191634 | MS.gene78382:CDS | 55.0% |
| !!! | GGGTTTTTGAGTGGTCCCAC+TGG | + | chr2.3:5190699-5190718 | MS.gene78382:CDS | 55.0% |
| !!! | TGGTCCCACTGGGGGTATTT+TGG | + | chr2.3:5190710-5190729 | MS.gene78382:CDS | 55.0% |
| GCTGAAGCAGAGAGAGCAGG+AGG | + | chr2.3:5191907-5191926 | MS.gene78382:CDS | 60.0% | |
| GGATAGGGAGCTGGAGAAGC+TGG | + | chr2.3:5191835-5191854 | MS.gene78382:CDS | 60.0% | |
| GGAGCTGGAGAAGCTGGCGA+TGG | + | chr2.3:5191841-5191860 | MS.gene78382:CDS | 65.0% | |
| TGTGTCGCCGCAGCAGTGTG+AGG | + | chr2.3:5191193-5191212 | MS.gene78382:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 5190672 | 5192027 | 5190672 | ID=MS.gene78382 |
| chr2.3 | mRNA | 5190672 | 5192027 | 5190672 | ID=MS.gene78382.t1;Parent=MS.gene78382 |
| chr2.3 | exon | 5190672 | 5192027 | 5190672 | ID=MS.gene78382.t1.exon1;Parent=MS.gene78382.t1 |
| chr2.3 | CDS | 5190672 | 5192027 | 5190672 | ID=cds.MS.gene78382.t1;Parent=MS.gene78382.t1 |
| Gene Sequence |
| Protein sequence |
