AlfalfaGEDB | MS.gene83309

M. sativa cultivar XinJiangDaYe / MS.gene83309

KEGG: K00001
GO: GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0008270 GO:0016491 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0055114
PFAM: PF00107.29 PF08240.15

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83309.t1	XP_003619782.1	98.6	352	5	0	1	352	26	377	3.10E-202	714.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83309.t1	Q8LEB2	78.1	352	77	0	1	352	30	381	6.2e-167	588.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83309.t1	G7KPC5	98.6	352	5	0	1	352	26	377	2.2e-202	714.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049807	MS.gene83309	0.818293	2.11E-52	-1.69E-46
MS.gene051885	MS.gene83309	0.828202	1.03E-54	-1.69E-46
MS.gene054554	MS.gene83309	0.814571	1.44E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene007227	MS.gene83309	PPI
MS.gene013678	MS.gene83309	PPI
MS.gene016853	MS.gene83309	PPI
MS.gene02655	MS.gene83309	PPI
MS.gene030545	MS.gene83309	PPI
MS.gene044228	MS.gene83309	PPI
MS.gene044229	MS.gene83309	PPI
MS.gene050322	MS.gene83309	PPI
MS.gene074415	MS.gene83309	PPI
MS.gene33522	MS.gene83309	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83309.t1	MTR_6g068930	98.580	352	5	0	1	352	26	377	0.0	715
MS.gene83309.t1	MTR_1g069430	51.977	354	166	2	1	350	24	377	1.43e-134	389
MS.gene83309.t1	MTR_7g099040	51.695	354	167	2	1	350	26	379	1.68e-133	386
MS.gene83309.t1	MTR_3g089940	50.847	354	170	2	1	350	25	378	1.84e-132	383
MS.gene83309.t1	MTR_3g089940	50.847	354	170	2	1	350	25	378	2.63e-132	383
MS.gene83309.t1	MTR_3g089977	50.565	354	171	2	1	350	25	378	8.27e-132	381
MS.gene83309.t1	MTR_3g089970	50.282	354	172	2	1	350	26	379	6.65e-131	379
MS.gene83309.t1	MTR_5g087710	50.000	358	173	4	1	352	34	391	2.68e-121	355
MS.gene83309.t1	MTR_3g089940	51.582	316	149	2	1	312	25	340	3.52e-116	341
MS.gene83309.t1	MTR_3g089940	51.582	316	149	2	1	312	25	340	3.84e-116	340

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83309.t1	AT5G24760	78.125	352	77	0	1	352	30	381	0.0	592
MS.gene83309.t1	AT5G24760	78.125	352	77	0	1	352	1	352	0.0	590
MS.gene83309.t1	AT5G24760	78.125	352	77	0	1	352	1	352	0.0	590
MS.gene83309.t1	AT5G24760	77.528	267	60	0	86	352	1	267	1.29e-160	450
MS.gene83309.t1	AT1G77120	53.239	355	160	3	1	350	24	377	1.31e-137	396
MS.gene83309.t1	AT5G43940	52.528	356	165	2	1	352	24	379	2.98e-136	393
MS.gene83309.t1	AT5G43940	52.528	356	165	2	1	352	36	391	4.52e-136	393
MS.gene83309.t1	AT1G64710	48.179	357	178	3	1	350	1	357	5.26e-121	353
MS.gene83309.t1	AT1G64710	48.179	357	178	3	1	350	24	380	1.91e-120	353
MS.gene83309.t1	AT5G42250	48.739	357	176	5	1	350	33	389	2.25e-118	348

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Find 73 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATGCATTCTTAGCTG+TGG	0.671818	6.1:-63032716	MS.gene83309:CDS
AAAGGAATTATCTTGGCATG+TGG	0.620375	6.1:+63030301	None:intergenic
AACTAATATTTGTCAAGTTT+TGG	0.171217	6.1:-63032897	MS.gene83309:CDS
AAGACAAGATTTACAGTAAA+AGG	0.163487	6.1:-63032839	MS.gene83309:CDS
AAGATCACTACGACAAAGAG+AGG	0.642771	6.1:+63034558	None:intergenic
AAGCCTGTTTATCATTATTG+CGG	0.594521	6.1:-63032815	MS.gene83309:CDS
AAGGATCTTTATTTGGAGGA+TGG	0.448842	6.1:-63031055	MS.gene83309:CDS
AATCCTCAGAAGTGTGAAAA+TGG	0.318657	6.1:-63031750	MS.gene83309:intron
AATGTTGCCGATGTATCAAA+AGG	0.534216	6.1:-63032054	MS.gene83309:CDS
ACAAGCCAAAGGAATTATCT+TGG	0.468138	6.1:+63030294	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATATGCATTCTTAGCTG+TGG	-	chr6.1:63032211-63032230	MS.gene83309:intron	30.0%
AACAGTAACTCAAGAGATTA+TGG	+	chr6.1:63033258-63033277	None:intergenic	30.0%
AAGCCTGTTTATCATTATTG+CGG	-	chr6.1:63032112-63032131	MS.gene83309:intron	30.0%
ACAGTAGTTATATTTGGTCT+TGG	-	chr6.1:63032900-63032919	MS.gene83309:CDS	30.0%
ACATACTTCTCTACTAATGA+AGG	+	chr6.1:63033916-63033935	None:intergenic	30.0%
ATAGGTTCTTTGTACAAACT+CGG	+	chr6.1:63033341-63033360	None:intergenic	30.0%
CGATAAGACAATATCAATAG+GGG	-	chr6.1:63034183-63034202	MS.gene83309:intron	30.0%
CTAAGGAATAAAAATCCACA+AGG	+	chr6.1:63034447-63034466	None:intergenic	30.0%
CTATAACAAAACATGACTTG+AGG	+	chr6.1:63033640-63033659	None:intergenic	30.0%
CTATGAAAACTGTTAACACA+TGG	+	chr6.1:63031973-63031992	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	63030308	63034641	63030308	ID=MS.gene83309
chr6.1	mRNA	63030308	63034641	63030308	ID=MS.gene83309.t1;Parent=MS.gene83309
chr6.1	exon	63034540	63034641	63034540	ID=MS.gene83309.t1.exon1;Parent=MS.gene83309.t1
chr6.1	CDS	63034540	63034641	63034540	ID=cds.MS.gene83309.t1;Parent=MS.gene83309.t1
chr6.1	exon	63033687	63033724	63033687	ID=MS.gene83309.t1.exon2;Parent=MS.gene83309.t1
chr6.1	CDS	63033687	63033724	63033687	ID=cds.MS.gene83309.t1;Parent=MS.gene83309.t1
chr6.1	exon	63032705	63033027	63032705	ID=MS.gene83309.t1.exon3;Parent=MS.gene83309.t1
chr6.1	CDS	63032705	63033027	63032705	ID=cds.MS.gene83309.t1;Parent=MS.gene83309.t1
chr6.1	exon	63032011	63032093	63032011	ID=MS.gene83309.t1.exon4;Parent=MS.gene83309.t1
chr6.1	CDS	63032011	63032093	63032011	ID=cds.MS.gene83309.t1;Parent=MS.gene83309.t1
chr6.1	exon	63031751	63031826	63031751	ID=MS.gene83309.t1.exon5;Parent=MS.gene83309.t1
chr6.1	CDS	63031751	63031826	63031751	ID=cds.MS.gene83309.t1;Parent=MS.gene83309.t1
chr6.1	exon	63031582	63031643	63031582	ID=MS.gene83309.t1.exon6;Parent=MS.gene83309.t1
chr6.1	CDS	63031582	63031643	63031582	ID=cds.MS.gene83309.t1;Parent=MS.gene83309.t1
chr6.1	exon	63031328	63031423	63031328	ID=MS.gene83309.t1.exon7;Parent=MS.gene83309.t1
chr6.1	CDS	63031328	63031423	63031328	ID=cds.MS.gene83309.t1;Parent=MS.gene83309.t1
chr6.1	exon	63031007	63031168	63031007	ID=MS.gene83309.t1.exon8;Parent=MS.gene83309.t1
chr6.1	CDS	63031007	63031168	63031007	ID=cds.MS.gene83309.t1;Parent=MS.gene83309.t1
chr6.1	exon	63030308	63030424	63030308	ID=MS.gene83309.t1.exon9;Parent=MS.gene83309.t1
chr6.1	CDS	63030308	63030424	63030308	ID=cds.MS.gene83309.t1;Parent=MS.gene83309.t1

Gene Sequence

>MS.gene83309

ATGGAGGAAGTGGAAGTCAGTCCTCCTCAACCATTGGAGATTAGGATTAAGGTTGTTTCCACCTCTCTTTGTCGTAGTGATCTTTCTGCTTGGGAAACTCATGTAAGCCTCTTATTTTATCATTATAGATTTAATTAATATACATGTTCAATGTGTAAGAAATCCTTGATATATATTGGTAGATCAAATACCGCGTTCAATGTAAATATTTTTACAATGTTGATCAATCATAATTGTCAAATCGTTAAAAAAGAAGATCGAATTTAATCATAACTATTTCTAAAGTCAAACATTAACGGTGTAAAATTTTATACGCCGATAATGTATCAAAATTAAACTATATCATCATATCCTGCCCTTTTTACTTCTTGTCTCCGATTTTATTATTTTCAATATCATGAATTTCCAATTAGTTGTTTTCTATAGATATTGTCGGGTCATCATACAAGAATTGGTCCTTTGTGAGATGTAATTGGATAAGATTATGCTTTTTTTCGTTTATGAGACAATGTATACCAATCAACATTTTGTTTGACATGATTAATGGTTAACTATGTAGCACCAATACTTCCAGATTAAAACTGTGTCTGACGCCAACACTCACGACCCCGACACGTATTGTTACTTCAGTCATTCCATTTTCTCAAATTATTATCGAGTGTTAGACATTTTAGTGTTACTGTCTATCGTGTCTGATGTCAGTTACTATGTCAGTGCTTCATATGTGATTAATCCTACATATTACAGTTCTTAAGAGTATCACTGCTTGTGTTGTTTGTAAATAAAAGCTAATGAAAGCTTGGTAGACTAGCCTGCTAATGAATATTTATTCTTTTTTCATAAATTATTCTACTTTTTTTATACTGAATATAAAACTAAATGGAATTACATCTAACATAAGGTGTTTTTATATGCAGGCCATATTTCCTCGCATATTTGGCCACGAAGCATCTGGGTAATCTACACTTAATGTTCTACTGTTGGATTAATTAAACAATAGCGATCCTTTAGTTGTTTTATTTGAAGATCTTAAATAGAATTTGTCTTTCTTAGAGTTTCACAATTATCTAGTATGAATTTATAATTAGATCAAATACTTAGAAACCAACGCTCAGTTAGAGTGACATTGATCAACTATGGGAATGGATTCCTTACGGTCAAAATATCACACACTACAGTAGATGAATCATTGGATGAAGATCAAATGACTCCCGTTTGAAATCCGATTTACCGAGTTTAAAATGAGAATCATCTGATTCCGTCCAACGATTCCAGCCTACTATCTGCAGTAAATGCGTGATACTATGACTGAAGGCAATCCAGGTCCATAAAAAATGGTAGGGTGCAGCAGAAGCACAGCTGAATGTTACTGATGTTAGACAGTGATGTATATGCATGAGAATGGGATAGTTGTAGCCAACTAGCCATAGCTGTAAATTGCGGTCTGCAAATGCAATTGCGGCACATTGGCTGCATTTGCCTGTATTTTGCTGTACCATTAAGGTTCACAATATAATTGTAACCGCAATTCACATAGACATGACTTTAACCAAATTATTTCAAAAGGTGTAATTTTCCATAGTAGATGTTGAATATAGTGTAATGGTTTTGCAGGATTGTTGAGAGCGTAGGGCTAGGAGTGACTGAATTCAAAGAAGGGGACCATGTGTTAACAGTTTTCATAGGAGAATGCATGTCATGCAGGCTGTGCAAATCAGGAAAAACTAATATTTGTCAAGTTTTGGGATTGGAAAGAAAGGGTTTAATGCATACTGATCAAAAGACAAGATTTACAGTAAAAGGGAAGCCTGTTTATCATTATTGCGGTGTTTCAAGTTTTAGTGAATACACAGTTGTTCATTCTGGATGTGCAGTGAAAGTCAGTCCTCATGTACCTCTTGAGAAAATATGCATTCTTAGCTGTGGTGTTTCTGCAGGTAAATATTTCCAACTTCCAAGTGTTTATTATTAAACTATAAGAAATTGAAAGTTAAGGTTTAAGGAAACTCTAGTAATCCCTTCGTCCTATATTGAGTGGCCTTTTAAGTTATCTCACACGAAGAGTTGTATGTAAATAAGTGAAAAAGTATAACTATTTTACAAAATTATCGTTATTAACTATTAGGTGCTAACCATTGTCATTGTAATTTGGACCGTAGTATTTCAATACAATTTGTACTCTGTAACCCTAACAATTGTATAATAGAAAACAACAACAAACACTTTCATGTCTGAGACGTAGGTATGATCAGCTAAACTCTGTGATCAATCGGTGTGTTCTTATGTGTTTATTTTTCTGTTTCTTTAGAGCTGGATTTGCTATTCTAACAGTTATAAATGCATAGTAGAGTATAATGAACTTGAGAGTGATGGCACATGATATTGATCTGAGGGTATAATCGCAATAAAATAATTAATATTATGTTGGCAACATAAAGTGATACTTATTATTTTAGGACATCTTTCTCAATGGAGAGCATTGAAATTCTAATGTCATTGATGAATTCGTTGTTGTTTATCATGTGACTCATTGCATTCCTAACATTAGGTCTAGGTGCAGCATGGAATGTTGCCGATGTATCAAAAGGATCAACAGTAGTTATATTTGGTCTTGGAACTGTTGGCCTTTCTGTAAGTCCAAGTACTCAACCACTATATCTATCTTATCTTCTGACGAGGATCTCAGAAGCACAAAGAGAATTCATATCTTAATAAATTAAAATATAAATATTGTTTGTGCTCTTGAGATCCTTATACTCTTCTCCACAGATGAATGCCATAACCTTTTCTAAATCATAACTTTCTTGTTCAATAGGTTGCACAAGGGGCCAAACTAAGGGGTGCATCTAGAATAATTGGTGTTGACAACAATCCTCAGAAGTGTGAAAATGGTAACATAATCAGTTCTCCGAATTACTCTGAATTTATTTTTAAACCTTAAAACGGGCCATAATCTCTTGAGTTACTGTTTGTTGTCTTATTCATTGCTACCACACAGCTAAATCTTTTGGGATTACAGAAGTTGTTGATCCGAGTTTGTACAAAGAACCTATCGCGCAGGTCGATTATTCATCTCATAAAATTTCCAATTTTACCTTTACAAATAATCTTATATACTTGTAATATATGACTATCAAAGTATATGATGCTAGATCACACGCACAACTGCACATAAGCTATAAATAACAATGCAACCATTTGTGTGTTGAAAATTTCAGGTTATTAAGAGCATTACTGATGGTGGGGCAGATTTCTGTTTTGAATGTGCAGGTGACACTGATATGATAACCACTGCATTGCAATCATGCTGTGATGTAAAACTCTATATCCCTCAAGTCATGTTTTGTTATAGAAATAGTTTATATATAAGCACTTGTACGATAACTGTTTGTATGCTATAAGCGCTTAATTAAGTTGTTTATCCAAACAGGGTCTGACTTGAATTTCATTCTATCTTTCCTTTTTTACAACAGGGGTGGGGCTTGACTGTAACACTTGGTGTGCCAAAGGTGAAGCCTGAGATGTCAGCTCATTATGGACTATTCCTGACAGGAAGAACTTTAAAAGGATCTTTATTTGGAGGATGGAAACCTAAAACTGATCTTCCTTCATTAGTAGAGAAGTATGTCAACAAGGTAAAGTGACAATATCTAGAATATATCCTTTATCTAAGCATGATTTTATATATTTTTACTTTATGGTAACACATGACAATGTCTACTTGATCACAATATCATTTCCCTTTTCTTGTTGTAACATACTTGTCTAACCAATACTTTTAAGCAAGACATTTTAGGCTGTTATTTTTGCTTACAGTTCTCTGCTACAATTTTTAATGTCATTTTGTTGTCTAAGTTATCATGGTCATTGGCCGACGATAAGACAATATCAATAGGGGTTGAGGCTAGAAGAAAGGACTAATCTCCCACAATGTGGCAAACCACCAATGTTCGAAACTGAATCTCTTTTGGGAGAAGTTTCCACATTGAACGTCCGACGATGTCTCACATATATGTGACTTAAATACATTCATAAATTTGTCCCCTACCCTTAACTTTTTAATATAAACATATGAAAGAGATTTTATTTTTGGATAAGGAAAAGTAATTCTAGTTACTTGCTTCATGTGCTTTTCTCCTTGTGGATTTTTATTCCTTAGGCAGTATTTATTAAGCATAAAAAAATATTTACTAATGATATGATAAATAAATATGCAGGAAATCCAGATTGATGACTACATAACACACAATTTGTCATTTGATGAAATTAACAAAGCTTTCAATCTCATGAAAGAAGGAAAGTGTCTTCGTTGTGTTATCCACATGCCAAGATAA

Protein sequence

>MS.gene83309.t1

MEEVEVSPPQPLEIRIKVVSTSLCRSDLSAWETHAIFPRIFGHEASGIVESVGLGVTEFKEGDHVLTVFIGECMSCRLCKSGKTNICQVLGLERKGLMHTDQKTRFTVKGKPVYHYCGVSSFSEYTVVHSGCAVKVSPHVPLEKICILSCGVSAGLGAAWNVADVSKGSTVVIFGLGTVGLSVAQGAKLRGASRIIGVDNNPQKCENAKSFGITEVVDPSLYKEPIAQVIKSITDGGADFCFECAGDTDMITTALQSCCDGWGLTVTLGVPKVKPEMSAHYGLFLTGRTLKGSLFGGWKPKTDLPSLVEKYVNKEIQIDDYITHNLSFDEINKAFNLMKEGKCLRCVIHMPR