AlfalfaGEDB | MS.gene70145

M. sativa cultivar XinJiangDaYe / MS.gene70145

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
PFAM: PF00107.29 PF08240.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70145.t1	XP_003630404.1	97.9	428	6	2	1	428	1	425	1.50E-238	835.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70145.t1	A4YGN0	43.9	369	197	5	57	425	1	359	7.7e-79	295.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70145.t1	G7LFT6	97.9	428	6	2	1	428	1	425	1.1e-238	835.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050170	MS.gene70145	-0.843944	1.03E-58	-1.69E-46
MS.gene050496	MS.gene70145	-0.800263	1.56E-48	-1.69E-46
MS.gene052486	MS.gene70145	-0.802062	6.71E-49	-1.69E-46
MS.gene053549	MS.gene70145	0.809553	1.79E-50	-1.69E-46
MS.gene05589	MS.gene70145	-0.803526	3.34E-49	-1.69E-46
MS.gene056504	MS.gene70145	-0.800062	1.72E-48	-1.69E-46
MS.gene06000	MS.gene70145	-0.81458	1.43E-51	-1.69E-46
MS.gene060187	MS.gene70145	0.825423	4.73E-54	-1.69E-46
MS.gene060595	MS.gene70145	0.833873	4.15E-56	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene013678	MS.gene70145	PPI
MS.gene78435	MS.gene70145	PPI
MS.gene81954	MS.gene70145	PPI
MS.gene013671	MS.gene70145	PPI
MS.gene78434	MS.gene70145	PPI
MS.gene77335	MS.gene70145	PPI
MS.gene81957	MS.gene70145	PPI
MS.gene81956	MS.gene70145	PPI
MS.gene70145	MS.gene79768	PPI
MS.gene84205	MS.gene70145	PPI
MS.gene038047	MS.gene70145	PPI
MS.gene044229	MS.gene70145	PPI
MS.gene069804	MS.gene70145	PPI
MS.gene39827	MS.gene70145	PPI
MS.gene02655	MS.gene70145	PPI
MS.gene37836	MS.gene70145	PPI
MS.gene02654	MS.gene70145	PPI
MS.gene84204	MS.gene70145	PPI
MS.gene83309	MS.gene70145	PPI
MS.gene016853	MS.gene70145	PPI
MS.gene074415	MS.gene70145	PPI
MS.gene042841	MS.gene70145	PPI
MS.gene33522	MS.gene70145	PPI
MS.gene70145	MS.gene02651	PPI
MS.gene77336	MS.gene70145	PPI
MS.gene044228	MS.gene70145	PPI
MS.gene02488	MS.gene70145	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70145.t1	MTR_8g095220	97.897	428	6	2	1	428	1	425	0.0	852
MS.gene70145.t1	MTR_8g095220	97.078	308	6	2	1	308	1	305	0.0	613
MS.gene70145.t1	MTR_7g099040	31.622	370	233	10	58	418	13	371	5.82e-46	163
MS.gene70145.t1	MTR_1g069430	31.063	367	237	10	58	417	11	368	6.12e-46	163
MS.gene70145.t1	MTR_3g089977	31.579	380	237	15	58	427	12	378	4.07e-45	161
MS.gene70145.t1	MTR_3g089940	31.316	380	238	15	58	427	12	378	1.54e-44	159
MS.gene70145.t1	MTR_3g089940	31.053	380	239	15	58	427	12	378	5.07e-44	158
MS.gene70145.t1	MTR_3g089970	30.952	378	238	15	60	427	15	379	9.91e-43	154
MS.gene70145.t1	MTR_3g089940	31.686	344	213	14	58	391	12	343	1.56e-40	148
MS.gene70145.t1	MTR_3g089940	31.395	344	214	14	58	391	12	343	5.56e-40	146
MS.gene70145.t1	MTR_5g087710	30.585	376	244	10	58	425	21	387	3.79e-38	142
MS.gene70145.t1	MTR_0001s0660	29.630	378	245	9	58	428	11	374	1.89e-36	139
MS.gene70145.t1	MTR_6g068930	29.600	375	247	11	58	427	13	375	8.94e-35	133
MS.gene70145.t1	MTR_7g068330	31.989	372	232	12	58	418	16	377	5.47e-34	131
MS.gene70145.t1	MTR_3g099460	27.979	386	261	10	51	427	12	389	1.30e-33	130
MS.gene70145.t1	MTR_5g045710	28.415	366	246	9	67	427	20	374	7.75e-33	127
MS.gene70145.t1	MTR_7g068320	31.551	374	232	15	58	418	23	385	4.48e-32	125
MS.gene70145.t1	MTR_3g099460	28.307	378	254	10	59	427	13	382	8.03e-32	125
MS.gene70145.t1	MTR_3g079180	28.042	378	254	11	50	418	8	376	3.08e-31	123
MS.gene70145.t1	MTR_6g465010	27.247	356	212	11	69	412	11	331	4.62e-28	114
MS.gene70145.t1	MTR_6g466320	27.247	356	212	11	69	412	27	347	4.82e-28	114
MS.gene70145.t1	MTR_6g465010	27.247	356	212	11	69	412	27	347	4.82e-28	114
MS.gene70145.t1	MTR_3g099460	28.136	295	198	7	51	338	12	299	5.29e-25	105
MS.gene70145.t1	MTR_5g045710	29.252	294	197	8	139	427	4	291	1.21e-24	103
MS.gene70145.t1	MTR_3g099460	27.469	324	221	8	110	427	11	326	1.33e-24	103
MS.gene70145.t1	MTR_3g099460	28.571	287	191	7	59	338	13	292	4.00e-23	99.8
MS.gene70145.t1	MTR_3g079170	31.330	233	150	8	194	419	54	283	2.87e-21	93.6
MS.gene70145.t1	MTR_4g115300	27.628	333	200	10	62	380	7	312	7.34e-20	90.9
MS.gene70145.t1	MTR_1g107425	25.538	372	241	10	59	427	13	351	3.65e-19	89.0
MS.gene70145.t1	MTR_5g031190	26.786	280	175	7	71	346	25	278	8.52e-19	87.8
MS.gene70145.t1	MTR_5g031305	28.188	298	180	10	54	346	11	279	1.86e-18	86.7
MS.gene70145.t1	MTR_8g068190	27.857	280	172	8	71	346	25	278	3.14e-18	85.9
MS.gene70145.t1	MTR_5g031360	26.849	365	223	14	71	427	27	355	4.35e-18	85.5
MS.gene70145.t1	MTR_5g031250	27.734	256	151	7	62	304	7	241	1.55e-17	82.4
MS.gene70145.t1	MTR_5g031430	25.862	348	222	10	83	427	39	353	1.67e-17	84.0
MS.gene70145.t1	MTR_5g031250	26.174	298	181	8	62	346	7	278	2.21e-17	83.6
MS.gene70145.t1	MTR_8g092630	29.825	228	132	8	70	292	81	285	2.54e-17	84.0
MS.gene70145.t1	MTR_5g031180	26.429	280	176	7	71	346	25	278	2.17e-16	80.5
MS.gene70145.t1	MTR_5g031310	26.421	299	181	8	61	346	7	279	2.52e-16	80.5
MS.gene70145.t1	MTR_5g031230	26.690	281	176	7	71	347	25	279	3.85e-16	79.7
MS.gene70145.t1	MTR_5g031300	26.842	380	236	13	54	427	11	354	3.88e-16	79.7
MS.gene70145.t1	MTR_5g031210	27.961	304	178	10	58	347	3	279	7.43e-16	79.0
MS.gene70145.t1	MTR_1g107425	29.098	244	148	6	59	299	13	234	2.14e-15	77.0
MS.gene70145.t1	MTR_2g009810	23.611	360	242	9	6	359	8	340	1.04e-14	75.9
MS.gene70145.t1	MTR_1g025950	25.373	268	169	8	83	346	38	278	5.94e-14	73.2
MS.gene70145.t1	MTR_5g031390	30.612	196	112	7	91	283	47	221	2.12e-13	71.6
MS.gene70145.t1	MTR_4g050760	24.036	337	192	10	57	388	1	278	9.06e-13	69.3
MS.gene70145.t1	MTR_2g012380	24.036	337	192	10	57	388	1	278	9.06e-13	69.3
MS.gene70145.t1	MTR_2g012380	23.824	319	180	9	57	370	1	261	1.01e-12	68.6
MS.gene70145.t1	MTR_4g029500	29.237	236	154	6	198	425	142	372	2.35e-12	68.6
MS.gene70145.t1	MTR_2g012420	27.604	192	134	5	199	389	21	208	1.72e-11	64.7
MS.gene70145.t1	MTR_4g050710	27.604	192	134	5	199	389	15	202	2.33e-11	63.9
MS.gene70145.t1	MTR_2g012420	27.749	191	133	5	199	388	92	278	9.26e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70145.t1	AT5G63620	74.246	431	102	3	1	428	3	427	0.0	660
MS.gene70145.t1	AT5G63620	73.782	431	104	3	1	428	3	427	0.0	656
MS.gene70145.t1	AT5G63620	71.935	310	78	3	1	307	3	306	1.27e-162	461
MS.gene70145.t1	AT5G43940	30.851	376	243	11	58	426	11	376	1.32e-45	162
MS.gene70145.t1	AT5G43940	31.016	374	241	11	60	426	25	388	3.62e-45	161
MS.gene70145.t1	AT1G77120	30.871	379	241	13	58	427	11	377	7.63e-44	157
MS.gene70145.t1	AT1G64710	29.076	368	248	7	58	418	11	372	2.15e-43	156
MS.gene70145.t1	AT4G22110	33.069	378	232	11	52	418	12	379	1.54e-42	154
MS.gene70145.t1	AT4G22110	33.069	378	232	11	52	418	12	379	1.54e-42	154
MS.gene70145.t1	AT1G64710	29.014	355	239	7	71	418	1	349	1.56e-41	150
MS.gene70145.t1	AT5G42250	29.067	375	251	9	54	420	16	383	1.30e-39	146
MS.gene70145.t1	AT1G22430	30.952	378	240	11	52	418	11	378	1.83e-38	143
MS.gene70145.t1	AT1G22430	30.952	378	240	11	52	418	11	378	1.83e-38	143
MS.gene70145.t1	AT5G24760	30.133	375	241	12	60	427	19	379	1.31e-37	140
MS.gene70145.t1	AT1G32780	29.351	385	249	12	58	427	12	388	2.45e-37	140
MS.gene70145.t1	AT5G24760	29.945	364	234	12	71	427	1	350	1.20e-35	135
MS.gene70145.t1	AT5G24760	29.945	364	234	12	71	427	1	350	1.20e-35	135
MS.gene70145.t1	AT1G22440	29.255	376	247	10	52	417	9	375	1.73e-35	135
MS.gene70145.t1	AT1G64710	29.210	291	200	5	133	418	14	303	1.35e-34	131
MS.gene70145.t1	AT4G22110	34.400	250	155	5	52	298	12	255	2.02e-29	116
MS.gene70145.t1	AT1G22430	31.541	279	178	7	52	324	11	282	2.67e-28	114
MS.gene70145.t1	AT1G22430	31.325	249	162	5	52	297	11	253	1.40e-26	108
MS.gene70145.t1	AT1G22430	31.325	249	162	5	52	297	11	253	1.40e-26	108
MS.gene70145.t1	AT5G51970	25.543	368	231	11	69	428	29	361	5.71e-26	108
MS.gene70145.t1	AT5G51970	25.543	368	231	11	69	428	29	361	5.71e-26	108
MS.gene70145.t1	AT5G24760	31.418	261	167	8	174	427	10	265	6.71e-25	103
MS.gene70145.t1	AT4G37970	25.905	359	228	10	74	427	29	354	5.50e-18	85.5
MS.gene70145.t1	AT4G37970	27.113	284	175	8	71	349	26	282	9.88e-18	83.6
MS.gene70145.t1	AT4G37990	26.433	314	195	10	71	378	21	304	2.50e-17	83.6
MS.gene70145.t1	AT4G37970	29.583	240	142	7	71	305	26	243	3.94e-17	81.3
MS.gene70145.t1	AT4G37970	29.583	240	142	7	71	305	26	243	4.55e-17	81.3
MS.gene70145.t1	AT2G21730	27.719	285	174	10	71	349	19	277	1.23e-16	81.6
MS.gene70145.t1	AT5G61510	26.333	300	162	7	69	366	41	283	1.07e-15	78.6
MS.gene70145.t1	AT2G21890	28.070	285	172	11	71	349	19	276	1.26e-15	78.6
MS.gene70145.t1	AT5G61510	26.333	300	162	7	69	366	98	340	1.71e-15	78.2
MS.gene70145.t1	AT1G72680	27.536	276	170	8	91	363	45	293	2.02e-14	74.7
MS.gene70145.t1	AT4G39330	27.682	289	177	11	66	349	20	281	1.31e-13	71.6
MS.gene70145.t1	AT4G39330	27.682	289	177	11	66	349	20	281	1.44e-13	72.0
MS.gene70145.t1	AT4G37980	25.962	312	199	11	71	378	21	304	2.15e-13	71.6
MS.gene70145.t1	AT3G19450	24.734	376	239	12	59	427	13	351	2.97e-13	71.2
MS.gene70145.t1	AT4G37980	25.714	280	178	9	71	346	21	274	6.90e-13	69.3
MS.gene70145.t1	AT4G21580	28.652	178	123	4	195	371	55	229	9.96e-13	68.9
MS.gene70145.t1	AT4G21580	27.000	200	141	5	195	392	88	284	1.50e-12	68.6

Find 102 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTAACCTTTCTATGGTTT+TGG	0.175052	4.1:-7481820	None:intergenic
GGTGATACTGTTGCTGTTAT+TGG	0.219498	4.1:+7482643	MS.gene70145:CDS
CTGGCACAAGCTGGTTCTTT+AGG	0.246946	4.1:+7483320	MS.gene70145:CDS
ACCGATATACATGTATAGTA+TGG	0.286726	4.1:+7482477	MS.gene70145:CDS
TGTGAACAGATAAAAGTTAT+TGG	0.299278	4.1:+7483897	MS.gene70145:intron
TCGAGTTTCACCATCGTATA+AGG	0.300970	4.1:-7482202	None:intergenic
ACTGTTGCTGTTATTGGAAC+TGG	0.304388	4.1:+7482649	MS.gene70145:CDS
GATGAGAAGTTCACCAGCTT+TGG	0.318763	4.1:-7481455	None:intergenic
TATACCGAGTCTGCAATTCT+AGG	0.324055	4.1:+7482565	MS.gene70145:CDS
TCGGTATATGGCATTGAGTT+TGG	0.337585	4.1:-7482550	None:intergenic
CCAATTGGATCTCGAGTTGT+TGG	0.344296	4.1:+7482002	MS.gene70145:CDS
TTCAATGGTGAGAGGTTTGT+TGG	0.348495	4.1:-7481413	None:intergenic
AACTTCACGCAGGAAATTAC+TGG	0.359885	4.1:+7483194	MS.gene70145:intron
GATGTTGCTGTTGAAGCCTT+AGG	0.362906	4.1:+7483230	MS.gene70145:CDS
TTAATTAGATTGGCAGAAAC+AGG	0.368222	4.1:+7483957	MS.gene70145:CDS
ATAAAAGTTATTGGCTCATA+TGG	0.373792	4.1:+7483906	MS.gene70145:CDS
ATGAGAAGTTCACCAGCTTT+GGG	0.377738	4.1:-7481454	None:intergenic
TATAATTGCTGTTGATGTTC+AGG	0.379502	4.1:+7482941	MS.gene70145:CDS
GGAGTCAGGCAAAGCATTCC+AGG	0.386069	4.1:+7484025	MS.gene70145:CDS
TCTTGAAAAGGTTGTCTTGT+TGG	0.386179	4.1:+7481263	MS.gene70145:CDS
TCTTCCAAAGTTAATTAGAT+TGG	0.387780	4.1:+7483947	MS.gene70145:CDS
CTGCAGATAGCTAGAGCATT+TGG	0.389366	4.1:+7482909	MS.gene70145:CDS
AGAATTGCAGACTCGGTATA+TGG	0.391325	4.1:-7482562	None:intergenic
GTTGTGGGCCATGAGATTAC+TGG	0.411881	4.1:+7481765	MS.gene70145:CDS
GATTGTTTCAGGCTTCCAAT+TGG	0.413947	4.1:+7481987	MS.gene70145:intron
CTGTTGCTGTTATTGGAACT+GGG	0.417859	4.1:+7482650	MS.gene70145:CDS
CCCATACTATACATGTATAT+CGG	0.418017	4.1:-7482478	None:intergenic
TCTGCCAATCTAATTAACTT+TGG	0.433236	4.1:-7483951	None:intergenic
GCTCATATGGAGGGAGGGCT+AGG	0.438318	4.1:+7483919	MS.gene70145:CDS
GGAGGAAAAGCAGTGATGAT+TGG	0.449002	4.1:+7483296	MS.gene70145:CDS
TGCAACTCACACAATAAATT+CGG	0.452277	4.1:+7482995	MS.gene70145:CDS
TTTGCTTATAACCGCGCAAA+AGG	0.453633	4.1:+7482177	MS.gene70145:CDS
ACCAACAGCTTCTGTGTGAA+TGG	0.454170	4.1:-7481344	None:intergenic
ACAGAAGCTGTTGGTGGTGT+TGG	0.457231	4.1:+7481352	MS.gene70145:CDS
CGACTGTTTCTTCGAGGAAG+TGG	0.460944	4.1:+7482222	MS.gene70145:CDS
GAAACAGGTATCTTCGACCT+TGG	0.461531	4.1:+7483972	MS.gene70145:CDS
TCAGCAACAACTTCTTGAAA+AGG	0.464826	4.1:+7481251	MS.gene70145:CDS
TCAATGGTGAGAGGTTTGTT+GGG	0.468644	4.1:-7481412	None:intergenic
AGCTAGAGCATTTGGTGCCT+CGG	0.473664	4.1:+7482917	MS.gene70145:CDS
CCAACAACTCGAGATCCAAT+TGG	0.477791	4.1:-7482002	None:intergenic
CATATTCCTCGTCCCAAAGC+TGG	0.483522	4.1:+7481442	MS.gene70145:CDS
AATACCGCCAATGCATTTGC+TGG	0.484950	4.1:-7482526	None:intergenic
AGTTCACCAGCTTTGGGACG+AGG	0.494387	4.1:-7481448	None:intergenic
CATGCAGCCGAAGTGCGCCC+TGG	0.499244	4.1:+7482622	MS.gene70145:CDS
ATGTTGCTGTTGAAGCCTTA+GGG	0.501604	4.1:+7483231	MS.gene70145:CDS
AGCTGGTTCTTTAGGAGAGG+TGG	0.503154	4.1:+7483328	MS.gene70145:CDS
CAATGTTACCTTTGAACAGT+AGG	0.517503	4.1:-7482054	None:intergenic
AGGAATATGAAATTCTTCAA+TGG	0.521033	4.1:-7481428	None:intergenic
TTCTTGTAACAGCATGGCCA+AGG	0.522537	4.1:-7483989	None:intergenic
AAATAGACTTGTTCGCAGAA+AGG	0.523418	4.1:+7483355	MS.gene70145:CDS
GAAACTCGACTGTTTCTTCG+AGG	0.525121	4.1:+7482216	MS.gene70145:CDS
CAGGAAATTACTGGTGGAAA+GGG	0.526933	4.1:+7483203	MS.gene70145:intron
ACAACACAAGGACTTGAAAA+CGG	0.537012	4.1:-7481747	None:intergenic
GCAGGAAATTACTGGTGGAA+AGG	0.554988	4.1:+7483202	MS.gene70145:intron
CCGATATACATGTATAGTAT+GGG	0.556704	4.1:+7482478	MS.gene70145:CDS
ACAGCACCACGCATGTAAGA+TGG	0.560649	4.1:-7481379	None:intergenic
GCAAAAGGAACCTTATACGA+TGG	0.560670	4.1:+7482192	MS.gene70145:CDS
CTGATTCCAAAACCATAGAA+AGG	0.561548	4.1:+7481814	MS.gene70145:CDS
CTAGAGAAAGCAAAGACACT+TGG	0.562324	4.1:+7482972	MS.gene70145:CDS
ATCTCATGGCCCACAACACA+AGG	0.562994	4.1:-7481759	None:intergenic
TCTGATCTTCATGTCATGAA+AGG	0.564745	4.1:+7481717	MS.gene70145:CDS
AAATGTCTGTGGTCTCCCTA+AGG	0.567546	4.1:-7483246	None:intergenic
TGCACACAAAGTGTGAAAGA+TGG	0.567900	4.1:+7483275	MS.gene70145:CDS
TCCATTCACACAGAAGCTGT+TGG	0.570592	4.1:+7481343	MS.gene70145:CDS
AAAGTTATTGGCTCATATGG+AGG	0.577253	4.1:+7483909	MS.gene70145:CDS
TGAAATTCTTCAATGGTGAG+AGG	0.583588	4.1:-7481421	None:intergenic
TGTTGAAGCTGATGCATGTA+CGG	0.583923	4.1:+7481318	MS.gene70145:CDS
TCACACAATAAATTCGGCTA+AGG	0.585756	4.1:+7483001	MS.gene70145:CDS
ATTCACACAGAAGCTGTTGG+TGG	0.585825	4.1:+7481346	MS.gene70145:CDS
AGTATCACCAGGGCGCACTT+CGG	0.593810	4.1:-7482629	None:intergenic
CAACTTCACCAGTAATCTCA+TGG	0.594487	4.1:-7481773	None:intergenic
ACAAGCTGGTTCTTTAGGAG+AGG	0.597825	4.1:+7483325	MS.gene70145:CDS
TGTTGCTGTTATTGGAACTG+GGG	0.602671	4.1:+7482651	MS.gene70145:CDS
CAACTGTTCCTACTGTTCAA+AGG	0.602922	4.1:+7482046	MS.gene70145:CDS
ACATCCTAGAATTGCAGACT+CGG	0.607209	4.1:-7482569	None:intergenic
AAGTTATTGGCTCATATGGA+GGG	0.607746	4.1:+7483910	MS.gene70145:CDS
GGAGCCTTCATTATGCCTTG+CGG	0.609397	4.1:+7482023	MS.gene70145:CDS
TGGCATCTGCACTAGTTCGT+AGG	0.610288	4.1:+7481221	MS.gene70145:CDS
TGTGCCAGCAAATGCATTGG+CGG	0.610816	4.1:+7482522	MS.gene70145:CDS
ATGATTGGACTGGCACAAGC+TGG	0.611907	4.1:+7483311	MS.gene70145:CDS
GTTGCTGTTATTGGAACTGG+GGG	0.615280	4.1:+7482652	MS.gene70145:CDS
ATATACATGTATAGTATGGG+AGG	0.616452	4.1:+7482481	MS.gene70145:CDS
ATAACAGCAACAGTATCACC+AGG	0.620053	4.1:-7482640	None:intergenic
AAGCTTCGCACAGATCATCA+TGG	0.622065	4.1:-7482152	None:intergenic
GTTGCCGCAAGGCATAATGA+AGG	0.623436	4.1:-7482027	None:intergenic
AAAAGCAGTGATGATTGGAC+TGG	0.624735	4.1:+7483301	MS.gene70145:CDS
GTGCACTGAGCAAATGTCTG+TGG	0.625723	4.1:-7483257	None:intergenic
CAGTAGGAACAGTTGCCGCA+AGG	0.626718	4.1:-7482038	None:intergenic
ACTGGTGAAGTTGTTGAACA+TGG	0.627651	4.1:+7481783	MS.gene70145:CDS
GAACTTCTCATCAAAACCAA+GGG	0.629940	4.1:+7481466	MS.gene70145:CDS
TTCACGCAGGAAATTACTGG+TGG	0.641213	4.1:+7483197	MS.gene70145:intron
TATACATGTATAGTATGGGA+GGG	0.642941	4.1:+7482482	MS.gene70145:CDS
TGAACTTCTCATCAAAACCA+AGG	0.647879	4.1:+7481465	MS.gene70145:CDS
TATTGGCTCATATGGAGGGA+GGG	0.648186	4.1:+7483914	MS.gene70145:CDS
TTATTGGCTCATATGGAGGG+AGG	0.648294	4.1:+7483913	MS.gene70145:CDS
ATCAACAGCAATTATATCCG+AGG	0.648309	4.1:-7482934	None:intergenic
TGTTGTGCCAGCAAATGCAT+TGG	0.658264	4.1:+7482519	MS.gene70145:CDS
GGATTGCCATCTTACATGCG+TGG	0.668555	4.1:+7481373	MS.gene70145:CDS
GCATTCCAGGATCTCAACGA+AGG	0.671434	4.1:+7484038	MS.gene70145:CDS
ACACAAAGTGTGAAAGATGG+AGG	0.689218	4.1:+7483278	MS.gene70145:CDS
TCATGACATGAAGATCAGAG+TGG	0.751085	4.1:-7481713	None:intergenic
TAACAGCAACAGTATCACCA+GGG	0.751466	4.1:-7482639	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATAATTGGCAAATTTAT+AGG	+	chr4.1:7482451-7482470	MS.gene70145:intron	15.0%
!!	TAGAGAAAACAATTAAATAA+AGG	-	chr4.1:7483175-7483194	None:intergenic	15.0%
!!!	ATTTTTGATATTGTTATGAT+TGG	+	chr4.1:7481628-7481647	MS.gene70145:intron	15.0%
!!	AAAAAACTTTAACCTTTCTA+TGG	-	chr4.1:7481829-7481848	None:intergenic	20.0%
!!	ATAAATTAAGCTTGTCTATA+TGG	-	chr4.1:7482320-7482339	None:intergenic	20.0%
!!	ATTGGAATACTGAAAATAAT+TGG	+	chr4.1:7482439-7482458	MS.gene70145:intron	20.0%
!!	GATTAAATTGTTCATATCAA+GGG	+	chr4.1:7483669-7483688	MS.gene70145:intron	20.0%
!!	TAAATTAAGCTTGTCTATAT+GGG	-	chr4.1:7482319-7482338	None:intergenic	20.0%
!!	TTTCAGTATTCCAATTATAA+AGG	-	chr4.1:7482434-7482453	None:intergenic	20.0%
!!!	ACATACAAATTTAATTTGCT+TGG	-	chr4.1:7483880-7483899	None:intergenic	20.0%
!!!	ATATACAAGTATCTTTTCAA+TGG	-	chr4.1:7483033-7483052	None:intergenic	20.0%
!!!	TATACAAGTATCTTTTCAAT+GGG	-	chr4.1:7483032-7483051	None:intergenic	20.0%
!	AAATGATTCAAGATAACCTT+TGG	+	chr4.1:7483646-7483665	MS.gene70145:intron	25.0%
!	AATGATTCAAGATAACCTTT+GGG	+	chr4.1:7483647-7483666	MS.gene70145:intron	25.0%
!	AGGAATATGAAATTCTTCAA+TGG	-	chr4.1:7481431-7481450	None:intergenic	25.0%
!	ATGAATTTGTCAAATAAGTG+AGG	-	chr4.1:7483857-7483876	None:intergenic	25.0%
!	GAAACAGCAAAAATAGAAAA+AGG	-	chr4.1:7482753-7482772	None:intergenic	25.0%
!	GGATTAAATTGTTCATATCA+AGG	+	chr4.1:7483668-7483687	MS.gene70145:intron	25.0%
!	TAACTAAATCAATTGTCAAG+TGG	+	chr4.1:7482688-7482707	MS.gene70145:intron	25.0%
!	TACAATCATTTCACATGTAT+CGG	-	chr4.1:7482283-7482302	None:intergenic	25.0%
!	TATGAACAATTTAATCCCAA+AGG	-	chr4.1:7483665-7483684	None:intergenic	25.0%
!	TCTTCCAAAGTTAATTAGAT+TGG	+	chr4.1:7483947-7483966	MS.gene70145:CDS	25.0%
!	TGTGAACAGATAAAAGTTAT+TGG	+	chr4.1:7483897-7483916	MS.gene70145:intron	25.0%
!	TTTGATATTGTTATGATTGG+TGG	+	chr4.1:7481631-7481650	MS.gene70145:intron	25.0%
!!	TACAAGAAAATACACTTTTG+AGG	+	chr4.1:7484004-7484023	MS.gene70145:CDS	25.0%
!!	TTATGATTGGTGGTTATTAT+TGG	+	chr4.1:7481641-7481660	MS.gene70145:intron	25.0%
!!!	ATAAAAGTTATTGGCTCATA+TGG	+	chr4.1:7483906-7483925	MS.gene70145:CDS	25.0%
	AAGCGGTGATAAAGTTAAAA+TGG	-	chr4.1:7482881-7482900	None:intergenic	30.0%
	ACCATAATTCAGTTGACAAA+TGG	-	chr4.1:7482096-7482115	None:intergenic	30.0%
	ACCGATATACATGTATAGTA+TGG	+	chr4.1:7482477-7482496	MS.gene70145:CDS	30.0%
	AGAGTAGACTCCTTTATAAT+TGG	+	chr4.1:7482421-7482440	MS.gene70145:intron	30.0%
	AGCAAAAAGGAATAACAAGT+AGG	+	chr4.1:7481955-7481974	MS.gene70145:intron	30.0%
	AGCATTATCACACAAAAATG+TGG	-	chr4.1:7481886-7481905	None:intergenic	30.0%
	ATATACATGTATAGTATGGG+AGG	+	chr4.1:7482481-7482500	MS.gene70145:CDS	30.0%
	CACTCAATTGAGTTTAAAAG+CGG	-	chr4.1:7483695-7483714	None:intergenic	30.0%
	CCCATACTATACATGTATAT+CGG	-	chr4.1:7482481-7482500	None:intergenic	30.0%
	CCGATATACATGTATAGTAT+GGG	+	chr4.1:7482478-7482497	MS.gene70145:CDS	30.0%
	GAATCAAGTAAGAAGTAAGA+GGG	-	chr4.1:7482252-7482271	None:intergenic	30.0%
	GACTATGTAAACACATACTT+AGG	-	chr4.1:7482344-7482363	None:intergenic	30.0%
	GCAAAAATAGAAAAAGGAAG+TGG	-	chr4.1:7482747-7482766	None:intergenic	30.0%
	TATACATGTATAGTATGGGA+GGG	+	chr4.1:7482482-7482501	MS.gene70145:CDS	30.0%
	TCCAACATGAATAAAACTAG+TGG	-	chr4.1:7483449-7483468	None:intergenic	30.0%
	TCCATTTGTCAACTGAATTA+TGG	+	chr4.1:7482092-7482111	MS.gene70145:intron	30.0%
	TCTGCCAATCTAATTAACTT+TGG	-	chr4.1:7483954-7483973	None:intergenic	30.0%
	TGAATCAAGTAAGAAGTAAG+AGG	-	chr4.1:7482253-7482272	None:intergenic	30.0%
	TGCAACTCACACAATAAATT+CGG	+	chr4.1:7482995-7483014	MS.gene70145:CDS	30.0%
	TGTATATTGCAGCTTAATTG+TGG	+	chr4.1:7481580-7481599	MS.gene70145:intron	30.0%
	TTAATTAGATTGGCAGAAAC+AGG	+	chr4.1:7483957-7483976	MS.gene70145:CDS	30.0%
	TTCGATAATGCTTGCTTAAA+TGG	+	chr4.1:7482770-7482789	MS.gene70145:intron	30.0%
	TTGTTCAACAGTTCAAGTAA+AGG	-	chr4.1:7483628-7483647	None:intergenic	30.0%
!	AGCTGCAATATACAAGTTTT+AGG	-	chr4.1:7481574-7481593	None:intergenic	30.0%
!	CTTTAACCTTTCTATGGTTT+TGG	-	chr4.1:7481823-7481842	None:intergenic	30.0%
!	TAAGAATTAGGTTGTACAAG+TGG	+	chr4.1:7483597-7483616	MS.gene70145:intron	30.0%
!	TATAATTGCTGTTGATGTTC+AGG	+	chr4.1:7482941-7482960	MS.gene70145:CDS	30.0%
!	TGTATTTTCTTGTAACAGCA+TGG	-	chr4.1:7483998-7484017	None:intergenic	30.0%
!!!	ACCACTAGTTTTATTCATGT+TGG	+	chr4.1:7483445-7483464	MS.gene70145:intron	30.0%
!!!	AGATGTGATTTTTTGAATGC+AGG	+	chr4.1:7481678-7481697	MS.gene70145:intron	30.0%
	ACAACACAAGGACTTGAAAA+CGG	-	chr4.1:7481750-7481769	None:intergenic	35.0%
	AGAGAGATAAACAATACCCT+TGG	-	chr4.1:7481485-7481504	None:intergenic	35.0%
	ATCAACAGCAATTATATCCG+AGG	-	chr4.1:7482937-7482956	None:intergenic	35.0%
	CAATGTTACCTTTGAACAGT+AGG	-	chr4.1:7482057-7482076	None:intergenic	35.0%
	CTCAACGAAGGAAAAATAGT+CGG	+	chr4.1:7484050-7484069	MS.gene70145:CDS	35.0%
	CTGATTCCAAAACCATAGAA+AGG	+	chr4.1:7481814-7481833	MS.gene70145:CDS	35.0%
	GAACTTCTCATCAAAACCAA+GGG	+	chr4.1:7481466-7481485	MS.gene70145:CDS	35.0%
	GGATTGTTCTTGATTGTTTC+AGG	+	chr4.1:7481976-7481995	MS.gene70145:intron	35.0%
	GTGTTTATGCTGTCTACAAA+CGG	+	chr4.1:7482120-7482139	MS.gene70145:intron	35.0%
	GTTCTGGCAATTAAACAATG+AGG	-	chr4.1:7483070-7483089	None:intergenic	35.0%
	TCACACAATAAATTCGGCTA+AGG	+	chr4.1:7483001-7483020	MS.gene70145:CDS	35.0%
	TCAGCAACAACTTCTTGAAA+AGG	+	chr4.1:7481251-7481270	MS.gene70145:CDS	35.0%
	TCTGATCTTCATGTCATGAA+AGG	+	chr4.1:7481717-7481736	MS.gene70145:CDS	35.0%
	TCTTGAAAAGGTTGTCTTGT+TGG	+	chr4.1:7481263-7481282	MS.gene70145:CDS	35.0%
	TGAAATTCTTCAATGGTGAG+AGG	-	chr4.1:7481424-7481443	None:intergenic	35.0%
	TGAACTTCTCATCAAAACCA+AGG	+	chr4.1:7481465-7481484	MS.gene70145:CDS	35.0%
	TGTCAACTGAATTATGGTCT+CGG	+	chr4.1:7482098-7482117	MS.gene70145:intron	35.0%
!	AAATAGACTTGTTCGCAGAA+AGG	+	chr4.1:7483355-7483374	MS.gene70145:CDS	35.0%
!	AGTCATAGTTTTCTCTGCTA+TGG	+	chr4.1:7482360-7482379	MS.gene70145:intron	35.0%
!	GTTCTTGATGACTAGCAAAA+AGG	+	chr4.1:7481942-7481961	MS.gene70145:intron	35.0%
!	TATTCCCATACCCCTAATTT+TGG	-	chr4.1:7483579-7483598	None:intergenic	35.0%
!	TTTTACTGCTTATGGTGCAA+TGG	+	chr4.1:7482597-7482616	MS.gene70145:CDS	35.0%
!	TTTTCATATATCCGAGCAGT+AGG	+	chr4.1:7482833-7482852	MS.gene70145:intron	35.0%
!!	AAAGTTATTGGCTCATATGG+AGG	+	chr4.1:7483909-7483928	MS.gene70145:CDS	35.0%
!!	AAATACACTTTTGAGGAGTC+AGG	+	chr4.1:7484011-7484030	MS.gene70145:CDS	35.0%
!!	AAGTTATTGGCTCATATGGA+GGG	+	chr4.1:7483910-7483929	MS.gene70145:CDS	35.0%
!!!	GATTTTTTGAATGCAGGATG+TGG	+	chr4.1:7481684-7481703	MS.gene70145:intron	35.0%
!!!	TGTGCTGTTTTTACTGCTTA+TGG	+	chr4.1:7482589-7482608	MS.gene70145:CDS	35.0%
!!!	TTTTCAAGTCCTTGTGTTGT+GGG	+	chr4.1:7481750-7481769	MS.gene70145:CDS	35.0%
	AAAAGCAGTGATGATTGGAC+TGG	+	chr4.1:7483301-7483320	MS.gene70145:CDS	40.0%
	AACTTCACGCAGGAAATTAC+TGG	+	chr4.1:7483194-7483213	MS.gene70145:intron	40.0%
	ACACAAAGTGTGAAAGATGG+AGG	+	chr4.1:7483278-7483297	MS.gene70145:CDS	40.0%
	ACATCCTAGAATTGCAGACT+CGG	-	chr4.1:7482572-7482591	None:intergenic	40.0%
	ACGAAGGAAAAATAGTCGGT+CGG	+	chr4.1:7484054-7484073	MS.gene70145:CDS	40.0%
	ACTGGTGAAGTTGTTGAACA+TGG	+	chr4.1:7481783-7481802	MS.gene70145:CDS	40.0%
	ACTTCACGTCAATTCTGCTT+TGG	-	chr4.1:7483780-7483799	None:intergenic	40.0%
	ATAACAGCAACAGTATCACC+AGG	-	chr4.1:7482643-7482662	None:intergenic	40.0%
	ATGAGAAGTTCACCAGCTTT+GGG	-	chr4.1:7481457-7481476	None:intergenic	40.0%
	CAACTGTTCCTACTGTTCAA+AGG	+	chr4.1:7482046-7482065	MS.gene70145:CDS	40.0%
	CAACTTCACCAGTAATCTCA+TGG	-	chr4.1:7481776-7481795	None:intergenic	40.0%
	CAGGAAATTACTGGTGGAAA+GGG	+	chr4.1:7483203-7483222	MS.gene70145:intron	40.0%
	CTAGAGAAAGCAAAGACACT+TGG	+	chr4.1:7482972-7482991	MS.gene70145:CDS	40.0%
	GATTGTTTCAGGCTTCCAAT+TGG	+	chr4.1:7481987-7482006	MS.gene70145:intron	40.0%
	GCAAAAGGAACCTTATACGA+TGG	+	chr4.1:7482192-7482211	MS.gene70145:CDS	40.0%
	GGGGTATGGGAATAAGAATT+AGG	+	chr4.1:7483585-7483604	MS.gene70145:intron	40.0%
	TAACAGCAACAGTATCACCA+GGG	-	chr4.1:7482642-7482661	None:intergenic	40.0%
	TATACCGAGTCTGCAATTCT+AGG	+	chr4.1:7482565-7482584	MS.gene70145:CDS	40.0%
	TCAAGCCAAAATTAGGGGTA+TGG	+	chr4.1:7483571-7483590	MS.gene70145:intron	40.0%
	TCATGACATGAAGATCAGAG+TGG	-	chr4.1:7481716-7481735	None:intergenic	40.0%
	TCGAGTTTCACCATCGTATA+AGG	-	chr4.1:7482205-7482224	None:intergenic	40.0%
	TGCACACAAAGTGTGAAAGA+TGG	+	chr4.1:7483275-7483294	MS.gene70145:CDS	40.0%
	TGTTAGGAGCTAATAGACAG+AGG	-	chr4.1:7483107-7483126	None:intergenic	40.0%
	TGTTGAAGCTGATGCATGTA+CGG	+	chr4.1:7481318-7481337	MS.gene70145:CDS	40.0%
	TTATGCACGATAGTGTGAGT+GGG	-	chr4.1:7483133-7483152	None:intergenic	40.0%
	TTCAATGGTGAGAGGTTTGT+TGG	-	chr4.1:7481416-7481435	None:intergenic	40.0%
	TTGCAGCTTAATTGTGGTTG+TGG	+	chr4.1:7481586-7481605	MS.gene70145:intron	40.0%
	TTTATGCACGATAGTGTGAG+TGG	-	chr4.1:7483134-7483153	None:intergenic	40.0%
	TTTGCTTATAACCGCGCAAA+AGG	+	chr4.1:7482177-7482196	MS.gene70145:CDS	40.0%
!	ACTGTTGCTGTTATTGGAAC+TGG	+	chr4.1:7482649-7482668	MS.gene70145:CDS	40.0%
!	AGAATTGCAGACTCGGTATA+TGG	-	chr4.1:7482565-7482584	None:intergenic	40.0%
!	ATGTTGCTGTTGAAGCCTTA+GGG	+	chr4.1:7483231-7483250	MS.gene70145:CDS	40.0%
!	CAAGCCAAAATTAGGGGTAT+GGG	+	chr4.1:7483572-7483591	MS.gene70145:intron	40.0%
!	CTGTTGCTGTTATTGGAACT+GGG	+	chr4.1:7482650-7482669	MS.gene70145:CDS	40.0%
!	GGTGATACTGTTGCTGTTAT+TGG	+	chr4.1:7482643-7482662	MS.gene70145:CDS	40.0%
!	TAAGTGAGGTGCTACACTAA+AGG	-	chr4.1:7483843-7483862	None:intergenic	40.0%
!	TCAATGGTGAGAGGTTTGTT+GGG	-	chr4.1:7481415-7481434	None:intergenic	40.0%
!	TCGGTATATGGCATTGAGTT+TGG	-	chr4.1:7482553-7482572	None:intergenic	40.0%
!	TGTTGCTGTTATTGGAACTG+GGG	+	chr4.1:7482651-7482670	MS.gene70145:CDS	40.0%
!	TGTTTTCTCTAACTTCACGC+AGG	+	chr4.1:7483184-7483203	MS.gene70145:intron	40.0%
!	TTTTTCCTTCGTTGAGATCC+TGG	-	chr4.1:7484046-7484065	None:intergenic	40.0%
!!!	GTTTTCAAGTCCTTGTGTTG+TGG	+	chr4.1:7481749-7481768	MS.gene70145:CDS	40.0%
	AAATGTCTGTGGTCTCCCTA+AGG	-	chr4.1:7483249-7483268	None:intergenic	45.0%
	AAGCTTCGCACAGATCATCA+TGG	-	chr4.1:7482155-7482174	None:intergenic	45.0%
	AATACCGCCAATGCATTTGC+TGG	-	chr4.1:7482529-7482548	None:intergenic	45.0%
	ACCAACAGCTTCTGTGTGAA+TGG	-	chr4.1:7481347-7481366	None:intergenic	45.0%
	ATTCACACAGAAGCTGTTGG+TGG	+	chr4.1:7481346-7481365	MS.gene70145:CDS	45.0%
	CCAACAACTCGAGATCCAAT+TGG	-	chr4.1:7482005-7482024	None:intergenic	45.0%
	CCCTGCTCAAGCCAAAATTA+GGG	+	chr4.1:7483565-7483584	MS.gene70145:intron	45.0%
	CCTGCTCAAGCCAAAATTAG+GGG	+	chr4.1:7483566-7483585	MS.gene70145:intron	45.0%
	CGAAGGAAAAATAGTCGGTC+GGG	+	chr4.1:7484055-7484074	MS.gene70145:CDS	45.0%
	CTGTCTACAAACGGTATGCA+GGG	+	chr4.1:7482129-7482148	MS.gene70145:intron	45.0%
	GAAACAGGTATCTTCGACCT+TGG	+	chr4.1:7483972-7483991	MS.gene70145:CDS	45.0%
	GATGAGAAGTTCACCAGCTT+TGG	-	chr4.1:7481458-7481477	None:intergenic	45.0%
	GCAGGAAATTACTGGTGGAA+AGG	+	chr4.1:7483202-7483221	MS.gene70145:intron	45.0%
	GGAGGAAAAGCAGTGATGAT+TGG	+	chr4.1:7483296-7483315	MS.gene70145:CDS	45.0%
	TAGTGTGAGTGGGTGATGTT+AGG	-	chr4.1:7483123-7483142	None:intergenic	45.0%
	TATCTGCAGACAACTGGAAG+CGG	-	chr4.1:7482898-7482917	None:intergenic	45.0%
	TCCATTCACACAGAAGCTGT+TGG	+	chr4.1:7481343-7481362	MS.gene70145:CDS	45.0%
	TCTAGCTATCTGCAGACAAC+TGG	-	chr4.1:7482904-7482923	None:intergenic	45.0%
	TGCCAGAACTCTATCTTGTG+CGG	+	chr4.1:7483081-7483100	MS.gene70145:intron	45.0%
	TGTTGTGCCAGCAAATGCAT+TGG	+	chr4.1:7482519-7482538	MS.gene70145:CDS	45.0%
	TTCACGCAGGAAATTACTGG+TGG	+	chr4.1:7483197-7483216	MS.gene70145:intron	45.0%
	TTCTTGTAACAGCATGGCCA+AGG	-	chr4.1:7483992-7484011	None:intergenic	45.0%
!	ACAAGCTGGTTCTTTAGGAG+AGG	+	chr4.1:7483325-7483344	MS.gene70145:CDS	45.0%
!	CCAATTGGATCTCGAGTTGT+TGG	+	chr4.1:7482002-7482021	MS.gene70145:CDS	45.0%
!	CGTATAAGGTTCCTTTTGCG+CGG	-	chr4.1:7482191-7482210	None:intergenic	45.0%
!	CTGCAGATAGCTAGAGCATT+TGG	+	chr4.1:7482909-7482928	MS.gene70145:CDS	45.0%
!	GAAACTCGACTGTTTCTTCG+AGG	+	chr4.1:7482216-7482235	MS.gene70145:CDS	45.0%
!	GATGTTGCTGTTGAAGCCTT+AGG	+	chr4.1:7483230-7483249	MS.gene70145:CDS	45.0%
!	GTTGCTGTTATTGGAACTGG+GGG	+	chr4.1:7482652-7482671	MS.gene70145:CDS	45.0%
!!	TATTGGCTCATATGGAGGGA+GGG	+	chr4.1:7483914-7483933	MS.gene70145:CDS	45.0%
!!	TTATTGGCTCATATGGAGGG+AGG	+	chr4.1:7483913-7483932	MS.gene70145:CDS	45.0%
!!!	ACATGCGTGGTGCTGTTTTT+CGG	+	chr4.1:7481386-7481405	MS.gene70145:CDS	45.0%
!!!	CCCTAATTTTGGCTTGAGCA+GGG	-	chr4.1:7483568-7483587	None:intergenic	45.0%
	ACAGCACCACGCATGTAAGA+TGG	-	chr4.1:7481382-7481401	None:intergenic	50.0%
	ATCTCATGGCCCACAACACA+AGG	-	chr4.1:7481762-7481781	None:intergenic	50.0%
	CATATTCCTCGTCCCAAAGC+TGG	+	chr4.1:7481442-7481461	MS.gene70145:CDS	50.0%
	GCATTCCAGGATCTCAACGA+AGG	+	chr4.1:7484038-7484057	MS.gene70145:CDS	50.0%
	GCCCTGCTCAAGCCAAAATT+AGG	+	chr4.1:7483564-7483583	MS.gene70145:intron	50.0%
	GCTGTCTACAAACGGTATGC+AGG	+	chr4.1:7482128-7482147	MS.gene70145:intron	50.0%
	GGAGCCTTCATTATGCCTTG+CGG	+	chr4.1:7482023-7482042	MS.gene70145:CDS	50.0%
	GGATTGCCATCTTACATGCG+TGG	+	chr4.1:7481373-7481392	MS.gene70145:CDS	50.0%
	GTGCACTGAGCAAATGTCTG+TGG	-	chr4.1:7483260-7483279	None:intergenic	50.0%
	GTTGATCAGAGCCTACTGCT+CGG	-	chr4.1:7482847-7482866	None:intergenic	50.0%
	GTTGCCGCAAGGCATAATGA+AGG	-	chr4.1:7482030-7482049	None:intergenic	50.0%
	GTTGTGGGCCATGAGATTAC+TGG	+	chr4.1:7481765-7481784	MS.gene70145:CDS	50.0%
	TGGCATCTGCACTAGTTCGT+AGG	+	chr4.1:7481221-7481240	MS.gene70145:CDS	50.0%
	TGTGCCAGCAAATGCATTGG+CGG	+	chr4.1:7482522-7482541	MS.gene70145:CDS	50.0%
!	AGCTGGTTCTTTAGGAGAGG+TGG	+	chr4.1:7483328-7483347	MS.gene70145:CDS	50.0%
!	CGACTGTTTCTTCGAGGAAG+TGG	+	chr4.1:7482222-7482241	MS.gene70145:CDS	50.0%
!	CTGGCACAAGCTGGTTCTTT+AGG	+	chr4.1:7483320-7483339	MS.gene70145:CDS	50.0%
!	GTTATTGGAACTGGGGGTGT+TGG	+	chr4.1:7482658-7482677	MS.gene70145:CDS	50.0%
!!	ACAGAAGCTGTTGGTGGTGT+TGG	+	chr4.1:7481352-7481371	MS.gene70145:CDS	50.0%
!!	AGCTAGAGCATTTGGTGCCT+CGG	+	chr4.1:7482917-7482936	MS.gene70145:CDS	50.0%
!!	ATGATTGGACTGGCACAAGC+TGG	+	chr4.1:7483311-7483330	MS.gene70145:CDS	50.0%
!!!	CATGCGTGGTGCTGTTTTTC+GGG	+	chr4.1:7481387-7481406	MS.gene70145:CDS	50.0%
!!!	CCCCTAATTTTGGCTTGAGC+AGG	-	chr4.1:7483569-7483588	None:intergenic	50.0%
	AGTATCACCAGGGCGCACTT+CGG	-	chr4.1:7482632-7482651	None:intergenic	55.0%
	CAGTAGGAACAGTTGCCGCA+AGG	-	chr4.1:7482041-7482060	None:intergenic	55.0%
	GGCCGCACAAGATAGAGTTC+TGG	-	chr4.1:7483086-7483105	None:intergenic	55.0%
!	GGAGTCAGGCAAAGCATTCC+AGG	+	chr4.1:7484025-7484044	MS.gene70145:CDS	55.0%
!!	AGTTCACCAGCTTTGGGACG+AGG	-	chr4.1:7481451-7481470	None:intergenic	55.0%
!!	GAACTGGGGGTGTTGGTTCT+AGG	+	chr4.1:7482665-7482684	MS.gene70145:CDS	55.0%
	GCTCATATGGAGGGAGGGCT+AGG	+	chr4.1:7483919-7483938	MS.gene70145:CDS	60.0%
	CATGCAGCCGAAGTGCGCCC+TGG	+	chr4.1:7482622-7482641	MS.gene70145:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	7481220	7484097	7481220	ID=MS.gene70145
chr4.1	mRNA	7481220	7484097	7481220	ID=MS.gene70145.t1;Parent=MS.gene70145
chr4.1	exon	7481220	7481487	7481220	ID=MS.gene70145.t1.exon1;Parent=MS.gene70145.t1
chr4.1	CDS	7481220	7481487	7481220	ID=cds.MS.gene70145.t1;Parent=MS.gene70145.t1
chr4.1	exon	7481700	7481835	7481700	ID=MS.gene70145.t1.exon2;Parent=MS.gene70145.t1
chr4.1	CDS	7481700	7481835	7481700	ID=cds.MS.gene70145.t1;Parent=MS.gene70145.t1
chr4.1	exon	7481998	7482067	7481998	ID=MS.gene70145.t1.exon3;Parent=MS.gene70145.t1
chr4.1	CDS	7481998	7482067	7481998	ID=cds.MS.gene70145.t1;Parent=MS.gene70145.t1
chr4.1	exon	7482150	7482243	7482150	ID=MS.gene70145.t1.exon4;Parent=MS.gene70145.t1
chr4.1	CDS	7482150	7482243	7482150	ID=cds.MS.gene70145.t1;Parent=MS.gene70145.t1
chr4.1	exon	7482473	7482686	7482473	ID=MS.gene70145.t1.exon5;Parent=MS.gene70145.t1
chr4.1	CDS	7482473	7482686	7482473	ID=cds.MS.gene70145.t1;Parent=MS.gene70145.t1
chr4.1	exon	7482905	7483046	7482905	ID=MS.gene70145.t1.exon6;Parent=MS.gene70145.t1
chr4.1	CDS	7482905	7483046	7482905	ID=cds.MS.gene70145.t1;Parent=MS.gene70145.t1
chr4.1	exon	7483206	7483376	7483206	ID=MS.gene70145.t1.exon7;Parent=MS.gene70145.t1
chr4.1	CDS	7483206	7483376	7483206	ID=cds.MS.gene70145.t1;Parent=MS.gene70145.t1
chr4.1	exon	7483906	7484097	7483906	ID=MS.gene70145.t1.exon8;Parent=MS.gene70145.t1
chr4.1	CDS	7483906	7484097	7483906	ID=cds.MS.gene70145.t1;Parent=MS.gene70145.t1

Gene Sequence

>MS.gene70145

ATGGCATCTGCACTAGTTCGTAGGAACATCATCAGCAACAACTTCTTGAAAAGGTTGTCTTGTTGGCGTTTGAATAATAACAAACAGTTGTTGAATAATGTTGAAGCTGATGCATGTACGGTTTCCATTCACACAGAAGCTGTTGGTGGTGTTGGATTGCCATCTTACATGCGTGGTGCTGTTTTTCGGGAACCCAACAAACCTCTCACCATTGAAGAATTTCATATTCCTCGTCCCAAAGCTGGTGAACTTCTCATCAAAACCAAGGGTATTGTTTATCTCTCTTGATGCTATATATATATATATTCTATTCATTTTTTAAATTAAAAATAATGACGATATGTTTCTGATCCTAAAACTTGTATATTGCAGCTTAATTGTGGTTGTGGATTGTAATTCAAAATTATTATTTTTGATATTGTTATGATTGGTGGTTATTATTGGATCTGATTGTGTAAAGATGTGATTTTTTGAATGCAGGATGTGGAGTATGCCACTCTGATCTTCATGTCATGAAAGGTGAAATTCCGTTTTCAAGTCCTTGTGTTGTGGGCCATGAGATTACTGGTGAAGTTGTTGAACATGGACAACACACTGATTCCAAAACCATAGAAAGGTTAAAGTTTTTTTTTTTTCTTTGTGTAATTGAAAACTGTCAAACTGCCACATTTTTGTGTGATAATGCTATGATGATGAGTTTGCATTCAATGAAAAGATTGATTGTTCTTGATGACTAGCAAAAAGGAATAACAAGTAGGATTGTTCTTGATTGTTTCAGGCTTCCAATTGGATCTCGAGTTGTTGGAGCCTTCATTATGCCTTGCGGCAACTGTTCCTACTGTTCAAAGGTAACATTGTATTACACACTCTACTCCATTTGTCAACTGAATTATGGTCTCGGTGTTTATGCTGTCTACAAACGGTATGCAGGGCCATGATGATCTGTGCGAAGCTTTTTTTGCTTATAACCGCGCAAAAGGAACCTTATACGATGGTGAAACTCGACTGTTTCTTCGAGGAAGTGGTGAACCCTCTTACTTCTTACTTGATTCACATATTACCGATACATGTGAAATGATTGTATGTTATCATTATTCCCATATAGACAAGCTTAATTTATACCTAAGTATGTGTTTACATAGTCATAGTTTTCTCTGCTATGGTATTTCATATTCAGCTGATACAAATTTCATATTTGTTGAGAGTAGACTCCTTTATAATTGGAATACTGAAAATAATTGGCAAATTTATAGGAAAACCGATATACATGTATAGTATGGGAGGGCTTGCTGAATACTGTGTTGTGCCAGCAAATGCATTGGCGGTATTGCCAAACTCAATGCCATATACCGAGTCTGCAATTCTAGGATGTGCTGTTTTTACTGCTTATGGTGCAATGGCTCATGCAGCCGAAGTGCGCCCTGGTGATACTGTTGCTGTTATTGGAACTGGGGGTGTTGGTTCTAGGTAACTAAATCAATTGTCAAGTGGATGTCACATTGTTAAATATTTAATCATCAGTGACCACTTCCTTTTTCTATTTTTGCTGTTTCGATAATGCTTGCTTAAATGGTCACTGATTTGAGAAGAAAACTCTTTATATTATGCAAAACTTTTCATATATCCGAGCAGTAGGCTCTGATCAACTGCACTTGTTTCCATTTTAACTTTATCACCGCTTCCAGTTGTCTGCAGATAGCTAGAGCATTTGGTGCCTCGGATATAATTGCTGTTGATGTTCAGGATGAAAAGCTAGAGAAAGCAAAGACACTTGGTGCAACTCACACAATAAATTCGGCTAAGGAAGATCCCATTGAAAAGATACTTGTATATGCATTTCTCTTTCGCCTCATTGTTTAATTGCCAGAACTCTATCTTGTGCGGCCTCTGTCTATTAGCTCCTAACATCACCCACTCACACTATCGTGCATAAATTATATCGCTTGAGAGATCCTTTATTTAATTGTTTTCTCTAACTTCACGCAGGAAATTACTGGTGGAAAGGGTGTTGATGTTGCTGTTGAAGCCTTAGGGAGACCACAGACATTTGCTCAGTGCACACAAAGTGTGAAAGATGGAGGAAAAGCAGTGATGATTGGACTGGCACAAGCTGGTTCTTTAGGAGAGGTGGATATAAATAGACTTGTTCGCAGAAAGGTAAGTGCTTTGTGTGTTGTATACATTGTCGATCGTTACTATAATTACTTTTTCTACTCTGACATATAACCACTAGTTTTATTCATGTTGGATTTAAACTGTTTAGCACACTTATCGTTATTAATGTTTGTTTTGTTCTAGAATTTGCTTCATTTTTTTCTGCGATTTATCGCAATGTCTTAATCTTGCCCTGCTCAAGCCAAAATTAGGGGTATGGGAATAAGAATTAGGTTGTACAAGTGGTTTTGCCTTTACTTGAACTGTTGAACAAATGATTCAAGATAACCTTTGGGATTAAATTGTTCATATCAAGGGCCGCTTTTAAACTCAATTGAGTGTTACATGTGAACAATATAGAGACTATAAATATGTAATATTTATTGATGATGCTAATTTTCTACCAAAGCAGAATTGACGTGAAGTGCTAAAACATAAAGTATACATTACTAAATGACAATAATTTCCTTTAGTGTAGCACCTCACTTATTTGACAAATTCATCCAAGCAAATTAAATTTGTATGTGAACAGATAAAAGTTATTGGCTCATATGGAGGGAGGGCTAGGCAAGATCTTCCAAAGTTAATTAGATTGGCAGAAACAGGTATCTTCGACCTTGGCCATGCTGTTACAAGAAAATACACTTTTGAGGAGTCAGGCAAAGCATTCCAGGATCTCAACGAAGGAAAAATAGTCGGTCGGGCTGTAATCGAGATAGTATAG

Protein sequence

>MS.gene70145.t1

MASALVRRNIISNNFLKRLSCWRLNNNKQLLNNVEADACTVSIHTEAVGGVGLPSYMRGAVFREPNKPLTIEEFHIPRPKAGELLIKTKGCGVCHSDLHVMKGEIPFSSPCVVGHEITGEVVEHGQHTDSKTIERLPIGSRVVGAFIMPCGNCSYCSKGHDDLCEAFFAYNRAKGTLYDGETRLFLRGSGKPIYMYSMGGLAEYCVVPANALAVLPNSMPYTESAILGCAVFTAYGAMAHAAEVRPGDTVAVIGTGGVGSSCLQIARAFGASDIIAVDVQDEKLEKAKTLGATHTINSAKEDPIEKILEITGGKGVDVAVEALGRPQTFAQCTQSVKDGGKAVMIGLAQAGSLGEVDINRLVRRKIKVIGSYGGRARQDLPKLIRLAETGIFDLGHAVTRKYTFEESGKAFQDLNEGKIVGRAVIEIV