AlfalfaGEDB | MS.gene49741

M. sativa cultivar XinJiangDaYe / MS.gene49741

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
PFAM: PF00107.29 PF08240.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49741.t1	XP_003630404.1	97.9	428	6	2	1	428	1	425	1.50E-238	835.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49741.t1	A4YGN0	43.9	369	197	5	57	425	1	359	7.7e-79	295.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49741.t1	G7LFT6	97.9	428	6	2	1	428	1	425	1.1e-238	835.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050170	MS.gene49741	-0.83488	2.32E-56	-1.69E-46
MS.gene053549	MS.gene49741	0.802803	4.72E-49	-1.69E-46
MS.gene06000	MS.gene49741	-0.805645	1.21E-49	-1.69E-46
MS.gene060187	MS.gene49741	0.820239	7.62E-53	-1.69E-46
MS.gene060595	MS.gene49741	0.825062	5.75E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene49741	MS.gene013671	PPI
MS.gene33522	MS.gene49741	PPI
MS.gene044228	MS.gene49741	PPI
MS.gene49741	MS.gene79768	PPI
MS.gene013678	MS.gene49741	PPI
MS.gene78435	MS.gene49741	PPI
MS.gene49741	MS.gene02488	PPI
MS.gene78434	MS.gene49741	PPI
MS.gene49741	MS.gene02654	PPI
MS.gene81957	MS.gene49741	PPI
MS.gene49741	MS.gene038047	PPI
MS.gene81956	MS.gene49741	PPI
MS.gene49741	MS.gene83309	PPI
MS.gene49741	MS.gene77336	PPI
MS.gene49741	MS.gene84205	PPI
MS.gene49741	MS.gene37836	PPI
MS.gene044229	MS.gene49741	PPI
MS.gene81954	MS.gene49741	PPI
MS.gene49741	MS.gene77335	PPI
MS.gene39827	MS.gene49741	PPI
MS.gene02655	MS.gene49741	PPI
MS.gene49741	MS.gene02651	PPI
MS.gene49741	MS.gene069804	PPI
MS.gene84204	MS.gene49741	PPI
MS.gene49741	MS.gene042841	PPI
MS.gene016853	MS.gene49741	PPI
MS.gene074415	MS.gene49741	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49741.t1	MTR_8g095220	97.897	428	6	2	1	428	1	425	0.0	852
MS.gene49741.t1	MTR_8g095220	97.078	308	6	2	1	308	1	305	0.0	613
MS.gene49741.t1	MTR_7g099040	31.622	370	233	10	58	418	13	371	5.82e-46	163
MS.gene49741.t1	MTR_1g069430	31.063	367	237	10	58	417	11	368	6.12e-46	163
MS.gene49741.t1	MTR_3g089977	31.579	380	237	15	58	427	12	378	4.07e-45	161
MS.gene49741.t1	MTR_3g089940	31.316	380	238	15	58	427	12	378	1.54e-44	159
MS.gene49741.t1	MTR_3g089940	31.053	380	239	15	58	427	12	378	5.07e-44	158
MS.gene49741.t1	MTR_3g089970	30.952	378	238	15	60	427	15	379	9.91e-43	154
MS.gene49741.t1	MTR_3g089940	31.686	344	213	14	58	391	12	343	1.56e-40	148
MS.gene49741.t1	MTR_3g089940	31.395	344	214	14	58	391	12	343	5.56e-40	146
MS.gene49741.t1	MTR_5g087710	30.585	376	244	10	58	425	21	387	3.79e-38	142
MS.gene49741.t1	MTR_0001s0660	29.630	378	245	9	58	428	11	374	1.89e-36	139
MS.gene49741.t1	MTR_6g068930	29.600	375	247	11	58	427	13	375	8.94e-35	133
MS.gene49741.t1	MTR_7g068330	31.989	372	232	12	58	418	16	377	5.47e-34	131
MS.gene49741.t1	MTR_3g099460	27.979	386	261	10	51	427	12	389	1.30e-33	130
MS.gene49741.t1	MTR_5g045710	28.415	366	246	9	67	427	20	374	7.75e-33	127
MS.gene49741.t1	MTR_7g068320	31.551	374	232	15	58	418	23	385	4.48e-32	125
MS.gene49741.t1	MTR_3g099460	28.307	378	254	10	59	427	13	382	8.03e-32	125
MS.gene49741.t1	MTR_3g079180	28.042	378	254	11	50	418	8	376	3.08e-31	123
MS.gene49741.t1	MTR_6g465010	27.247	356	212	11	69	412	11	331	4.62e-28	114
MS.gene49741.t1	MTR_6g466320	27.247	356	212	11	69	412	27	347	4.82e-28	114
MS.gene49741.t1	MTR_6g465010	27.247	356	212	11	69	412	27	347	4.82e-28	114
MS.gene49741.t1	MTR_3g099460	28.136	295	198	7	51	338	12	299	5.29e-25	105
MS.gene49741.t1	MTR_5g045710	29.252	294	197	8	139	427	4	291	1.21e-24	103
MS.gene49741.t1	MTR_3g099460	27.469	324	221	8	110	427	11	326	1.33e-24	103
MS.gene49741.t1	MTR_3g099460	28.571	287	191	7	59	338	13	292	4.00e-23	99.8
MS.gene49741.t1	MTR_3g079170	31.330	233	150	8	194	419	54	283	2.87e-21	93.6
MS.gene49741.t1	MTR_4g115300	27.628	333	200	10	62	380	7	312	7.34e-20	90.9
MS.gene49741.t1	MTR_1g107425	25.538	372	241	10	59	427	13	351	3.65e-19	89.0
MS.gene49741.t1	MTR_5g031190	26.786	280	175	7	71	346	25	278	8.52e-19	87.8
MS.gene49741.t1	MTR_5g031305	28.188	298	180	10	54	346	11	279	1.86e-18	86.7
MS.gene49741.t1	MTR_8g068190	27.857	280	172	8	71	346	25	278	3.14e-18	85.9
MS.gene49741.t1	MTR_5g031360	26.849	365	223	14	71	427	27	355	4.35e-18	85.5
MS.gene49741.t1	MTR_5g031250	27.734	256	151	7	62	304	7	241	1.55e-17	82.4
MS.gene49741.t1	MTR_5g031430	25.862	348	222	10	83	427	39	353	1.67e-17	84.0
MS.gene49741.t1	MTR_5g031250	26.174	298	181	8	62	346	7	278	2.21e-17	83.6
MS.gene49741.t1	MTR_8g092630	29.825	228	132	8	70	292	81	285	2.54e-17	84.0
MS.gene49741.t1	MTR_5g031180	26.429	280	176	7	71	346	25	278	2.17e-16	80.5
MS.gene49741.t1	MTR_5g031310	26.421	299	181	8	61	346	7	279	2.52e-16	80.5
MS.gene49741.t1	MTR_5g031230	26.690	281	176	7	71	347	25	279	3.85e-16	79.7
MS.gene49741.t1	MTR_5g031300	26.842	380	236	13	54	427	11	354	3.88e-16	79.7
MS.gene49741.t1	MTR_5g031210	27.961	304	178	10	58	347	3	279	7.43e-16	79.0
MS.gene49741.t1	MTR_1g107425	29.098	244	148	6	59	299	13	234	2.14e-15	77.0
MS.gene49741.t1	MTR_2g009810	23.611	360	242	9	6	359	8	340	1.04e-14	75.9
MS.gene49741.t1	MTR_1g025950	25.373	268	169	8	83	346	38	278	5.94e-14	73.2
MS.gene49741.t1	MTR_5g031390	30.612	196	112	7	91	283	47	221	2.12e-13	71.6
MS.gene49741.t1	MTR_4g050760	24.036	337	192	10	57	388	1	278	9.06e-13	69.3
MS.gene49741.t1	MTR_2g012380	24.036	337	192	10	57	388	1	278	9.06e-13	69.3
MS.gene49741.t1	MTR_2g012380	23.824	319	180	9	57	370	1	261	1.01e-12	68.6
MS.gene49741.t1	MTR_4g029500	29.237	236	154	6	198	425	142	372	2.35e-12	68.6
MS.gene49741.t1	MTR_2g012420	27.604	192	134	5	199	389	21	208	1.72e-11	64.7
MS.gene49741.t1	MTR_4g050710	27.604	192	134	5	199	389	15	202	2.33e-11	63.9
MS.gene49741.t1	MTR_2g012420	27.749	191	133	5	199	388	92	278	9.26e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49741.t1	AT5G63620	74.246	431	102	3	1	428	3	427	0.0	660
MS.gene49741.t1	AT5G63620	73.782	431	104	3	1	428	3	427	0.0	656
MS.gene49741.t1	AT5G63620	71.935	310	78	3	1	307	3	306	1.27e-162	461
MS.gene49741.t1	AT5G43940	30.851	376	243	11	58	426	11	376	1.32e-45	162
MS.gene49741.t1	AT5G43940	31.016	374	241	11	60	426	25	388	3.62e-45	161
MS.gene49741.t1	AT1G77120	30.871	379	241	13	58	427	11	377	7.63e-44	157
MS.gene49741.t1	AT1G64710	29.076	368	248	7	58	418	11	372	2.15e-43	156
MS.gene49741.t1	AT4G22110	33.069	378	232	11	52	418	12	379	1.54e-42	154
MS.gene49741.t1	AT4G22110	33.069	378	232	11	52	418	12	379	1.54e-42	154
MS.gene49741.t1	AT1G64710	29.014	355	239	7	71	418	1	349	1.56e-41	150
MS.gene49741.t1	AT5G42250	29.067	375	251	9	54	420	16	383	1.30e-39	146
MS.gene49741.t1	AT1G22430	30.952	378	240	11	52	418	11	378	1.83e-38	143
MS.gene49741.t1	AT1G22430	30.952	378	240	11	52	418	11	378	1.83e-38	143
MS.gene49741.t1	AT5G24760	30.133	375	241	12	60	427	19	379	1.31e-37	140
MS.gene49741.t1	AT1G32780	29.351	385	249	12	58	427	12	388	2.45e-37	140
MS.gene49741.t1	AT5G24760	29.945	364	234	12	71	427	1	350	1.20e-35	135
MS.gene49741.t1	AT5G24760	29.945	364	234	12	71	427	1	350	1.20e-35	135
MS.gene49741.t1	AT1G22440	29.255	376	247	10	52	417	9	375	1.73e-35	135
MS.gene49741.t1	AT1G64710	29.210	291	200	5	133	418	14	303	1.35e-34	131
MS.gene49741.t1	AT4G22110	34.400	250	155	5	52	298	12	255	2.02e-29	116
MS.gene49741.t1	AT1G22430	31.541	279	178	7	52	324	11	282	2.67e-28	114
MS.gene49741.t1	AT1G22430	31.325	249	162	5	52	297	11	253	1.40e-26	108
MS.gene49741.t1	AT1G22430	31.325	249	162	5	52	297	11	253	1.40e-26	108
MS.gene49741.t1	AT5G51970	25.543	368	231	11	69	428	29	361	5.71e-26	108
MS.gene49741.t1	AT5G51970	25.543	368	231	11	69	428	29	361	5.71e-26	108
MS.gene49741.t1	AT5G24760	31.418	261	167	8	174	427	10	265	6.71e-25	103
MS.gene49741.t1	AT4G37970	25.905	359	228	10	74	427	29	354	5.50e-18	85.5
MS.gene49741.t1	AT4G37970	27.113	284	175	8	71	349	26	282	9.88e-18	83.6
MS.gene49741.t1	AT4G37990	26.433	314	195	10	71	378	21	304	2.50e-17	83.6
MS.gene49741.t1	AT4G37970	29.583	240	142	7	71	305	26	243	3.94e-17	81.3
MS.gene49741.t1	AT4G37970	29.583	240	142	7	71	305	26	243	4.55e-17	81.3
MS.gene49741.t1	AT2G21730	27.719	285	174	10	71	349	19	277	1.23e-16	81.6
MS.gene49741.t1	AT5G61510	26.333	300	162	7	69	366	41	283	1.07e-15	78.6
MS.gene49741.t1	AT2G21890	28.070	285	172	11	71	349	19	276	1.26e-15	78.6
MS.gene49741.t1	AT5G61510	26.333	300	162	7	69	366	98	340	1.71e-15	78.2
MS.gene49741.t1	AT1G72680	27.536	276	170	8	91	363	45	293	2.02e-14	74.7
MS.gene49741.t1	AT4G39330	27.682	289	177	11	66	349	20	281	1.31e-13	71.6
MS.gene49741.t1	AT4G39330	27.682	289	177	11	66	349	20	281	1.44e-13	72.0
MS.gene49741.t1	AT4G37980	25.962	312	199	11	71	378	21	304	2.15e-13	71.6
MS.gene49741.t1	AT3G19450	24.734	376	239	12	59	427	13	351	2.97e-13	71.2
MS.gene49741.t1	AT4G37980	25.714	280	178	9	71	346	21	274	6.90e-13	69.3
MS.gene49741.t1	AT4G21580	28.652	178	123	4	195	371	55	229	9.96e-13	68.9
MS.gene49741.t1	AT4G21580	27.000	200	141	5	195	392	88	284	1.50e-12	68.6

Find 102 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTAACCTTTCTATGGTTT+TGG	0.175052	4.4:-7573207	None:intergenic
GGTGATACTGTTGCTGTTAT+TGG	0.219498	4.4:+7574029	MS.gene49741:CDS
CTGGCACAAGCTGGTTCTTT+AGG	0.246946	4.4:+7574706	MS.gene49741:CDS
ACCGATATACATGTATAGTA+TGG	0.286726	4.4:+7573863	MS.gene49741:CDS
TGTGAACAGATAAAAGTTAT+TGG	0.299278	4.4:+7575283	MS.gene49741:intron
TCGAGTTTCACCATCGTATA+AGG	0.300970	4.4:-7573588	None:intergenic
ACTGTTGCTGTTATTGGAAC+TGG	0.304388	4.4:+7574035	MS.gene49741:CDS
GATGAGAAGTTCACCAGCTT+TGG	0.318763	4.4:-7572842	None:intergenic
TATACCGAGTCTGCAATTCT+AGG	0.324055	4.4:+7573951	MS.gene49741:CDS
TCGGTATATGGCATTGAGTT+TGG	0.337585	4.4:-7573936	None:intergenic
CCAATTGGATCTCGAGTTGT+TGG	0.344296	4.4:+7573388	MS.gene49741:CDS
TTCAATGGTGAGAGGTTTGT+TGG	0.348495	4.4:-7572800	None:intergenic
AACTTCACGCAGGAAATTAC+TGG	0.359885	4.4:+7574580	MS.gene49741:intron
GATGTTGCTGTTGAAGCCTT+AGG	0.362906	4.4:+7574616	MS.gene49741:CDS
TTAATTAGATTGGCAGAAAC+AGG	0.368222	4.4:+7575343	MS.gene49741:CDS
ATAAAAGTTATTGGCTCATA+TGG	0.373792	4.4:+7575292	MS.gene49741:CDS
ATGAGAAGTTCACCAGCTTT+GGG	0.377738	4.4:-7572841	None:intergenic
TATAATTGCTGTTGATGTTC+AGG	0.379502	4.4:+7574327	MS.gene49741:CDS
GGAGTCAGGCAAAGCATTCC+AGG	0.386069	4.4:+7575411	MS.gene49741:CDS
TCTTGAAAAGGTTGTCTTGT+TGG	0.386179	4.4:+7572650	MS.gene49741:CDS
TCTTCCAAAGTTAATTAGAT+TGG	0.387780	4.4:+7575333	MS.gene49741:CDS
CTGCAGATAGCTAGAGCATT+TGG	0.389366	4.4:+7574295	MS.gene49741:CDS
AGAATTGCAGACTCGGTATA+TGG	0.391325	4.4:-7573948	None:intergenic
GTTGTGGGCCATGAGATTAC+TGG	0.411881	4.4:+7573152	MS.gene49741:CDS
GATTGTTTCAGGCTTCCAAT+TGG	0.413947	4.4:+7573373	MS.gene49741:intron
CTGTTGCTGTTATTGGAACT+GGG	0.417859	4.4:+7574036	MS.gene49741:CDS
CCCATACTATACATGTATAT+CGG	0.418017	4.4:-7573864	None:intergenic
TCTGCCAATCTAATTAACTT+TGG	0.433236	4.4:-7575337	None:intergenic
GCTCATATGGAGGGAGGGCT+AGG	0.438318	4.4:+7575305	MS.gene49741:CDS
GGAGGAAAAGCAGTGATGAT+TGG	0.449002	4.4:+7574682	MS.gene49741:CDS
TGCAACTCACACAATAAATT+CGG	0.452277	4.4:+7574381	MS.gene49741:CDS
TTTGCTTATAACCGCGCAAA+AGG	0.453633	4.4:+7573563	MS.gene49741:CDS
ACCAACAGCTTCTGTGTGAA+TGG	0.454170	4.4:-7572731	None:intergenic
ACAGAAGCTGTTGGTGGTGT+TGG	0.457231	4.4:+7572739	MS.gene49741:CDS
CGACTGTTTCTTCGAGGAAG+TGG	0.460944	4.4:+7573608	MS.gene49741:CDS
GAAACAGGTATCTTCGACCT+TGG	0.461531	4.4:+7575358	MS.gene49741:CDS
TCAGCAACAACTTCTTGAAA+AGG	0.464826	4.4:+7572638	MS.gene49741:CDS
TCAATGGTGAGAGGTTTGTT+GGG	0.468644	4.4:-7572799	None:intergenic
AGCTAGAGCATTTGGTGCCT+CGG	0.473664	4.4:+7574303	MS.gene49741:CDS
CCAACAACTCGAGATCCAAT+TGG	0.477791	4.4:-7573388	None:intergenic
CATATTCCTCGTCCCAAAGC+TGG	0.483522	4.4:+7572829	MS.gene49741:CDS
AATACCGCCAATGCATTTGC+TGG	0.484950	4.4:-7573912	None:intergenic
AGTTCACCAGCTTTGGGACG+AGG	0.494387	4.4:-7572835	None:intergenic
CATGCAGCCGAAGTGCGCCC+TGG	0.499244	4.4:+7574008	MS.gene49741:CDS
ATGTTGCTGTTGAAGCCTTA+GGG	0.501604	4.4:+7574617	MS.gene49741:CDS
AGCTGGTTCTTTAGGAGAGG+TGG	0.503154	4.4:+7574714	MS.gene49741:CDS
CAATGTTACCTTTGAACAGT+AGG	0.517503	4.4:-7573440	None:intergenic
AGGAATATGAAATTCTTCAA+TGG	0.521033	4.4:-7572815	None:intergenic
TTCTTGTAACAGCATGGCCA+AGG	0.522537	4.4:-7575375	None:intergenic
AAATAGACTTGTTCGCAGAA+AGG	0.523418	4.4:+7574741	MS.gene49741:CDS
GAAACTCGACTGTTTCTTCG+AGG	0.525121	4.4:+7573602	MS.gene49741:CDS
CAGGAAATTACTGGTGGAAA+GGG	0.526933	4.4:+7574589	MS.gene49741:intron
ACAACACAAGGACTTGAAAA+CGG	0.537012	4.4:-7573134	None:intergenic
GCAGGAAATTACTGGTGGAA+AGG	0.554988	4.4:+7574588	MS.gene49741:intron
CCGATATACATGTATAGTAT+GGG	0.556704	4.4:+7573864	MS.gene49741:CDS
ACAGCACCACGCATGTAAGA+TGG	0.560649	4.4:-7572766	None:intergenic
GCAAAAGGAACCTTATACGA+TGG	0.560670	4.4:+7573578	MS.gene49741:CDS
CTGATTCCAAAACCATAGAA+AGG	0.561548	4.4:+7573201	MS.gene49741:CDS
CTAGAGAAAGCAAAGACACT+TGG	0.562324	4.4:+7574358	MS.gene49741:CDS
ATCTCATGGCCCACAACACA+AGG	0.562994	4.4:-7573146	None:intergenic
TCTGATCTTCATGTCATGAA+AGG	0.564745	4.4:+7573104	MS.gene49741:CDS
AAATGTCTGTGGTCTCCCTA+AGG	0.567546	4.4:-7574632	None:intergenic
TGCACACAAAGTGTGAAAGA+TGG	0.567900	4.4:+7574661	MS.gene49741:CDS
TCCATTCACACAGAAGCTGT+TGG	0.570592	4.4:+7572730	MS.gene49741:CDS
AAAGTTATTGGCTCATATGG+AGG	0.577253	4.4:+7575295	MS.gene49741:CDS
TGAAATTCTTCAATGGTGAG+AGG	0.583588	4.4:-7572808	None:intergenic
TGTTGAAGCTGATGCATGTA+CGG	0.583923	4.4:+7572705	MS.gene49741:CDS
TCACACAATAAATTCGGCTA+AGG	0.585756	4.4:+7574387	MS.gene49741:CDS
ATTCACACAGAAGCTGTTGG+TGG	0.585825	4.4:+7572733	MS.gene49741:CDS
AGTATCACCAGGGCGCACTT+CGG	0.593810	4.4:-7574015	None:intergenic
CAACTTCACCAGTAATCTCA+TGG	0.594487	4.4:-7573160	None:intergenic
ACAAGCTGGTTCTTTAGGAG+AGG	0.597825	4.4:+7574711	MS.gene49741:CDS
TGTTGCTGTTATTGGAACTG+GGG	0.602671	4.4:+7574037	MS.gene49741:CDS
CAACTGTTCCTACTGTTCAA+AGG	0.602922	4.4:+7573432	MS.gene49741:CDS
ACATCCTAGAATTGCAGACT+CGG	0.607209	4.4:-7573955	None:intergenic
AAGTTATTGGCTCATATGGA+GGG	0.607746	4.4:+7575296	MS.gene49741:CDS
GGAGCCTTCATTATGCCTTG+CGG	0.609397	4.4:+7573409	MS.gene49741:CDS
TGGCATCTGCACTAGTTCGT+AGG	0.610288	4.4:+7572608	MS.gene49741:CDS
TGTGCCAGCAAATGCATTGG+CGG	0.610816	4.4:+7573908	MS.gene49741:CDS
ATGATTGGACTGGCACAAGC+TGG	0.611907	4.4:+7574697	MS.gene49741:CDS
GTTGCTGTTATTGGAACTGG+GGG	0.615280	4.4:+7574038	MS.gene49741:CDS
ATATACATGTATAGTATGGG+AGG	0.616452	4.4:+7573867	MS.gene49741:CDS
ATAACAGCAACAGTATCACC+AGG	0.620053	4.4:-7574026	None:intergenic
AAGCTTCGCACAGATCATCA+TGG	0.622065	4.4:-7573538	None:intergenic
GTTGCCGCAAGGCATAATGA+AGG	0.623436	4.4:-7573413	None:intergenic
AAAAGCAGTGATGATTGGAC+TGG	0.624735	4.4:+7574687	MS.gene49741:CDS
GTGCACTGAGCAAATGTCTG+TGG	0.625723	4.4:-7574643	None:intergenic
CAGTAGGAACAGTTGCCGCA+AGG	0.626718	4.4:-7573424	None:intergenic
ACTGGTGAAGTTGTTGAACA+TGG	0.627651	4.4:+7573170	MS.gene49741:CDS
GAACTTCTCATCAAAACCAA+GGG	0.629940	4.4:+7572853	MS.gene49741:CDS
TTCACGCAGGAAATTACTGG+TGG	0.641213	4.4:+7574583	MS.gene49741:intron
TATACATGTATAGTATGGGA+GGG	0.642941	4.4:+7573868	MS.gene49741:CDS
TGAACTTCTCATCAAAACCA+AGG	0.647879	4.4:+7572852	MS.gene49741:CDS
TATTGGCTCATATGGAGGGA+GGG	0.648186	4.4:+7575300	MS.gene49741:CDS
TTATTGGCTCATATGGAGGG+AGG	0.648294	4.4:+7575299	MS.gene49741:CDS
ATCAACAGCAATTATATCCG+AGG	0.648309	4.4:-7574320	None:intergenic
TGTTGTGCCAGCAAATGCAT+TGG	0.658264	4.4:+7573905	MS.gene49741:CDS
GGATTGCCATCTTACATGCG+TGG	0.668555	4.4:+7572760	MS.gene49741:CDS
GCATTCCAGGATCTCAACGA+AGG	0.671434	4.4:+7575424	MS.gene49741:CDS
ACACAAAGTGTGAAAGATGG+AGG	0.689218	4.4:+7574664	MS.gene49741:CDS
TCATGACATGAAGATCAGAG+TGG	0.751085	4.4:-7573100	None:intergenic
TAACAGCAACAGTATCACCA+GGG	0.751466	4.4:-7574025	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATAATTGGCAAATTTAT+AGG	+	chr4.4:7573837-7573856	MS.gene49741:intron	15.0%
!!	TAGAGAAAACAATTAAATAA+AGG	-	chr4.4:7574561-7574580	None:intergenic	15.0%
!!!	ATTTTTGATATTGTTATGAT+TGG	+	chr4.4:7573015-7573034	MS.gene49741:intron	15.0%
!!	AAAAAACTTTAACCTTTCTA+TGG	-	chr4.4:7573216-7573235	None:intergenic	20.0%
!!	ATAAATTAAGCTTGTCTATA+TGG	-	chr4.4:7573706-7573725	None:intergenic	20.0%
!!	ATTGGAATACTGAAAATAAT+TGG	+	chr4.4:7573825-7573844	MS.gene49741:intron	20.0%
!!	GATTAAATTGTTCATATCAA+GGG	+	chr4.4:7575055-7575074	MS.gene49741:intron	20.0%
!!	TAAATTAAGCTTGTCTATAT+GGG	-	chr4.4:7573705-7573724	None:intergenic	20.0%
!!	TTTCAGTATTCCAATTATAA+AGG	-	chr4.4:7573820-7573839	None:intergenic	20.0%
!!!	ACATACAAATTTAATTTGCT+TGG	-	chr4.4:7575266-7575285	None:intergenic	20.0%
!!!	ATATACAAGTATCTTTTCAA+TGG	-	chr4.4:7574419-7574438	None:intergenic	20.0%
!!!	TATACAAGTATCTTTTCAAT+GGG	-	chr4.4:7574418-7574437	None:intergenic	20.0%
!	AAATGATTCAAGATAACCTT+TGG	+	chr4.4:7575032-7575051	MS.gene49741:intron	25.0%
!	AATGATTCAAGATAACCTTT+GGG	+	chr4.4:7575033-7575052	MS.gene49741:intron	25.0%
!	AGGAATATGAAATTCTTCAA+TGG	-	chr4.4:7572818-7572837	None:intergenic	25.0%
!	ATGAATTTGTCAAATAAGTG+AGG	-	chr4.4:7575243-7575262	None:intergenic	25.0%
!	GAAACAGCAAAAATAGAAAA+AGG	-	chr4.4:7574139-7574158	None:intergenic	25.0%
!	GGATTAAATTGTTCATATCA+AGG	+	chr4.4:7575054-7575073	MS.gene49741:intron	25.0%
!	TAACTAAATCAATTGTCAAG+TGG	+	chr4.4:7574074-7574093	MS.gene49741:intron	25.0%
!	TACAATCATTTCACATGTAT+CGG	-	chr4.4:7573669-7573688	None:intergenic	25.0%
!	TATGAACAATTTAATCCCAA+AGG	-	chr4.4:7575051-7575070	None:intergenic	25.0%
!	TCTTCCAAAGTTAATTAGAT+TGG	+	chr4.4:7575333-7575352	MS.gene49741:CDS	25.0%
!	TGTGAACAGATAAAAGTTAT+TGG	+	chr4.4:7575283-7575302	MS.gene49741:intron	25.0%
!	TTTGATATTGTTATGATTGG+TGG	+	chr4.4:7573018-7573037	MS.gene49741:intron	25.0%
!!	TACAAGAAAATACACTTTTG+AGG	+	chr4.4:7575390-7575409	MS.gene49741:CDS	25.0%
!!	TTATGATTGGTGGTTATTAT+TGG	+	chr4.4:7573028-7573047	MS.gene49741:intron	25.0%
!!!	ATAAAAGTTATTGGCTCATA+TGG	+	chr4.4:7575292-7575311	MS.gene49741:CDS	25.0%
	AAGCGGTGATAAAGTTAAAA+TGG	-	chr4.4:7574267-7574286	None:intergenic	30.0%
	ACCATAATTCAGTTGACAAA+TGG	-	chr4.4:7573482-7573501	None:intergenic	30.0%
	ACCGATATACATGTATAGTA+TGG	+	chr4.4:7573863-7573882	MS.gene49741:CDS	30.0%
	AGAGTAGACTCCTTTATAAT+TGG	+	chr4.4:7573807-7573826	MS.gene49741:intron	30.0%
	AGCAAAAAGGAATAACAAGT+AGG	+	chr4.4:7573341-7573360	MS.gene49741:intron	30.0%
	AGCATTATCACACAAAAATG+TGG	-	chr4.4:7573272-7573291	None:intergenic	30.0%
	ATATACATGTATAGTATGGG+AGG	+	chr4.4:7573867-7573886	MS.gene49741:CDS	30.0%
	CACTCAATTGAGTTTAAAAG+CGG	-	chr4.4:7575081-7575100	None:intergenic	30.0%
	CCCATACTATACATGTATAT+CGG	-	chr4.4:7573867-7573886	None:intergenic	30.0%
	CCGATATACATGTATAGTAT+GGG	+	chr4.4:7573864-7573883	MS.gene49741:CDS	30.0%
	GAATCAAGTAAGAAGTAAGA+GGG	-	chr4.4:7573638-7573657	None:intergenic	30.0%
	GACTATGTAAACACATACTT+AGG	-	chr4.4:7573730-7573749	None:intergenic	30.0%
	GCAAAAATAGAAAAAGGAAG+TGG	-	chr4.4:7574133-7574152	None:intergenic	30.0%
	TATACATGTATAGTATGGGA+GGG	+	chr4.4:7573868-7573887	MS.gene49741:CDS	30.0%
	TCCAACATGAATAAAACTAG+TGG	-	chr4.4:7574835-7574854	None:intergenic	30.0%
	TCCATTTGTCAACTGAATTA+TGG	+	chr4.4:7573478-7573497	MS.gene49741:intron	30.0%
	TCTGCCAATCTAATTAACTT+TGG	-	chr4.4:7575340-7575359	None:intergenic	30.0%
	TGAATCAAGTAAGAAGTAAG+AGG	-	chr4.4:7573639-7573658	None:intergenic	30.0%
	TGCAACTCACACAATAAATT+CGG	+	chr4.4:7574381-7574400	MS.gene49741:CDS	30.0%
	TGTATATTGCAGCTTAATTG+TGG	+	chr4.4:7572967-7572986	MS.gene49741:intron	30.0%
	TTAATTAGATTGGCAGAAAC+AGG	+	chr4.4:7575343-7575362	MS.gene49741:CDS	30.0%
	TTCGATAATGCTTGCTTAAA+TGG	+	chr4.4:7574156-7574175	MS.gene49741:intron	30.0%
	TTGTTCAACAGTTCAAGTAA+AGG	-	chr4.4:7575014-7575033	None:intergenic	30.0%
!	AGCTGCAATATACAAGTTTT+AGG	-	chr4.4:7572961-7572980	None:intergenic	30.0%
!	CTTTAACCTTTCTATGGTTT+TGG	-	chr4.4:7573210-7573229	None:intergenic	30.0%
!	TAAGAATTAGGTTGTACAAG+TGG	+	chr4.4:7574983-7575002	MS.gene49741:intron	30.0%
!	TATAATTGCTGTTGATGTTC+AGG	+	chr4.4:7574327-7574346	MS.gene49741:CDS	30.0%
!	TGTATTTTCTTGTAACAGCA+TGG	-	chr4.4:7575384-7575403	None:intergenic	30.0%
!!!	ACCACTAGTTTTATTCATGT+TGG	+	chr4.4:7574831-7574850	MS.gene49741:intron	30.0%
!!!	AGATGTGATTTTTTGAATGC+AGG	+	chr4.4:7573065-7573084	MS.gene49741:intron	30.0%
	ACAACACAAGGACTTGAAAA+CGG	-	chr4.4:7573137-7573156	None:intergenic	35.0%
	AGAGAGATAAACAATACCCT+TGG	-	chr4.4:7572872-7572891	None:intergenic	35.0%
	ATCAACAGCAATTATATCCG+AGG	-	chr4.4:7574323-7574342	None:intergenic	35.0%
	CAATGTTACCTTTGAACAGT+AGG	-	chr4.4:7573443-7573462	None:intergenic	35.0%
	CTCAACGAAGGAAAAATAGT+CGG	+	chr4.4:7575436-7575455	MS.gene49741:CDS	35.0%
	CTGATTCCAAAACCATAGAA+AGG	+	chr4.4:7573201-7573220	MS.gene49741:CDS	35.0%
	GAACTTCTCATCAAAACCAA+GGG	+	chr4.4:7572853-7572872	MS.gene49741:CDS	35.0%
	GGATTGTTCTTGATTGTTTC+AGG	+	chr4.4:7573362-7573381	MS.gene49741:intron	35.0%
	GTGTTTATGCTGTCTACAAA+CGG	+	chr4.4:7573506-7573525	MS.gene49741:intron	35.0%
	GTTCTGGCAATTAAACAATG+AGG	-	chr4.4:7574456-7574475	None:intergenic	35.0%
	TCACACAATAAATTCGGCTA+AGG	+	chr4.4:7574387-7574406	MS.gene49741:CDS	35.0%
	TCAGCAACAACTTCTTGAAA+AGG	+	chr4.4:7572638-7572657	MS.gene49741:CDS	35.0%
	TCTGATCTTCATGTCATGAA+AGG	+	chr4.4:7573104-7573123	MS.gene49741:CDS	35.0%
	TCTTGAAAAGGTTGTCTTGT+TGG	+	chr4.4:7572650-7572669	MS.gene49741:CDS	35.0%
	TGAAATTCTTCAATGGTGAG+AGG	-	chr4.4:7572811-7572830	None:intergenic	35.0%
	TGAACTTCTCATCAAAACCA+AGG	+	chr4.4:7572852-7572871	MS.gene49741:CDS	35.0%
	TGTCAACTGAATTATGGTCT+CGG	+	chr4.4:7573484-7573503	MS.gene49741:intron	35.0%
!	AAATAGACTTGTTCGCAGAA+AGG	+	chr4.4:7574741-7574760	MS.gene49741:CDS	35.0%
!	AGTCATAGTTTTCTCTGCTA+TGG	+	chr4.4:7573746-7573765	MS.gene49741:intron	35.0%
!	GTTCTTGATGACTAGCAAAA+AGG	+	chr4.4:7573328-7573347	MS.gene49741:intron	35.0%
!	TATTCCCATACCCCTAATTT+TGG	-	chr4.4:7574965-7574984	None:intergenic	35.0%
!	TTTTACTGCTTATGGTGCAA+TGG	+	chr4.4:7573983-7574002	MS.gene49741:CDS	35.0%
!	TTTTCATATATCCGAGCAGT+AGG	+	chr4.4:7574219-7574238	MS.gene49741:intron	35.0%
!!	AAAGTTATTGGCTCATATGG+AGG	+	chr4.4:7575295-7575314	MS.gene49741:CDS	35.0%
!!	AAATACACTTTTGAGGAGTC+AGG	+	chr4.4:7575397-7575416	MS.gene49741:CDS	35.0%
!!	AAGTTATTGGCTCATATGGA+GGG	+	chr4.4:7575296-7575315	MS.gene49741:CDS	35.0%
!!!	GATTTTTTGAATGCAGGATG+TGG	+	chr4.4:7573071-7573090	MS.gene49741:intron	35.0%
!!!	TGTGCTGTTTTTACTGCTTA+TGG	+	chr4.4:7573975-7573994	MS.gene49741:CDS	35.0%
!!!	TTTTCAAGTCCTTGTGTTGT+GGG	+	chr4.4:7573137-7573156	MS.gene49741:CDS	35.0%
	AAAAGCAGTGATGATTGGAC+TGG	+	chr4.4:7574687-7574706	MS.gene49741:CDS	40.0%
	AACTTCACGCAGGAAATTAC+TGG	+	chr4.4:7574580-7574599	MS.gene49741:intron	40.0%
	ACACAAAGTGTGAAAGATGG+AGG	+	chr4.4:7574664-7574683	MS.gene49741:CDS	40.0%
	ACATCCTAGAATTGCAGACT+CGG	-	chr4.4:7573958-7573977	None:intergenic	40.0%
	ACGAAGGAAAAATAGTCGGT+CGG	+	chr4.4:7575440-7575459	MS.gene49741:CDS	40.0%
	ACTGGTGAAGTTGTTGAACA+TGG	+	chr4.4:7573170-7573189	MS.gene49741:CDS	40.0%
	ACTTCACGTCAATTCTGCTT+TGG	-	chr4.4:7575166-7575185	None:intergenic	40.0%
	ATAACAGCAACAGTATCACC+AGG	-	chr4.4:7574029-7574048	None:intergenic	40.0%
	ATGAGAAGTTCACCAGCTTT+GGG	-	chr4.4:7572844-7572863	None:intergenic	40.0%
	CAACTGTTCCTACTGTTCAA+AGG	+	chr4.4:7573432-7573451	MS.gene49741:CDS	40.0%
	CAACTTCACCAGTAATCTCA+TGG	-	chr4.4:7573163-7573182	None:intergenic	40.0%
	CAGGAAATTACTGGTGGAAA+GGG	+	chr4.4:7574589-7574608	MS.gene49741:intron	40.0%
	CTAGAGAAAGCAAAGACACT+TGG	+	chr4.4:7574358-7574377	MS.gene49741:CDS	40.0%
	GATTGTTTCAGGCTTCCAAT+TGG	+	chr4.4:7573373-7573392	MS.gene49741:intron	40.0%
	GCAAAAGGAACCTTATACGA+TGG	+	chr4.4:7573578-7573597	MS.gene49741:CDS	40.0%
	GGGGTATGGGAATAAGAATT+AGG	+	chr4.4:7574971-7574990	MS.gene49741:intron	40.0%
	TAACAGCAACAGTATCACCA+GGG	-	chr4.4:7574028-7574047	None:intergenic	40.0%
	TATACCGAGTCTGCAATTCT+AGG	+	chr4.4:7573951-7573970	MS.gene49741:CDS	40.0%
	TCAAGCCAAAATTAGGGGTA+TGG	+	chr4.4:7574957-7574976	MS.gene49741:intron	40.0%
	TCATGACATGAAGATCAGAG+TGG	-	chr4.4:7573103-7573122	None:intergenic	40.0%
	TCGAGTTTCACCATCGTATA+AGG	-	chr4.4:7573591-7573610	None:intergenic	40.0%
	TGCACACAAAGTGTGAAAGA+TGG	+	chr4.4:7574661-7574680	MS.gene49741:CDS	40.0%
	TGTTAGGAGCTAATAGACAG+AGG	-	chr4.4:7574493-7574512	None:intergenic	40.0%
	TGTTGAAGCTGATGCATGTA+CGG	+	chr4.4:7572705-7572724	MS.gene49741:CDS	40.0%
	TTATGCACGATAGTGTGAGT+GGG	-	chr4.4:7574519-7574538	None:intergenic	40.0%
	TTCAATGGTGAGAGGTTTGT+TGG	-	chr4.4:7572803-7572822	None:intergenic	40.0%
	TTGCAGCTTAATTGTGGTTG+TGG	+	chr4.4:7572973-7572992	MS.gene49741:intron	40.0%
	TTTATGCACGATAGTGTGAG+TGG	-	chr4.4:7574520-7574539	None:intergenic	40.0%
	TTTGCTTATAACCGCGCAAA+AGG	+	chr4.4:7573563-7573582	MS.gene49741:CDS	40.0%
!	ACTGTTGCTGTTATTGGAAC+TGG	+	chr4.4:7574035-7574054	MS.gene49741:CDS	40.0%
!	AGAATTGCAGACTCGGTATA+TGG	-	chr4.4:7573951-7573970	None:intergenic	40.0%
!	ATGTTGCTGTTGAAGCCTTA+GGG	+	chr4.4:7574617-7574636	MS.gene49741:CDS	40.0%
!	CAAGCCAAAATTAGGGGTAT+GGG	+	chr4.4:7574958-7574977	MS.gene49741:intron	40.0%
!	CTGTTGCTGTTATTGGAACT+GGG	+	chr4.4:7574036-7574055	MS.gene49741:CDS	40.0%
!	GGTGATACTGTTGCTGTTAT+TGG	+	chr4.4:7574029-7574048	MS.gene49741:CDS	40.0%
!	TAAGTGAGGTGCTACACTAA+AGG	-	chr4.4:7575229-7575248	None:intergenic	40.0%
!	TCAATGGTGAGAGGTTTGTT+GGG	-	chr4.4:7572802-7572821	None:intergenic	40.0%
!	TCGGTATATGGCATTGAGTT+TGG	-	chr4.4:7573939-7573958	None:intergenic	40.0%
!	TGTTGCTGTTATTGGAACTG+GGG	+	chr4.4:7574037-7574056	MS.gene49741:CDS	40.0%
!	TGTTTTCTCTAACTTCACGC+AGG	+	chr4.4:7574570-7574589	MS.gene49741:intron	40.0%
!	TTTTTCCTTCGTTGAGATCC+TGG	-	chr4.4:7575432-7575451	None:intergenic	40.0%
!!!	GTTTTCAAGTCCTTGTGTTG+TGG	+	chr4.4:7573136-7573155	MS.gene49741:CDS	40.0%
	AAATGTCTGTGGTCTCCCTA+AGG	-	chr4.4:7574635-7574654	None:intergenic	45.0%
	AAGCTTCGCACAGATCATCA+TGG	-	chr4.4:7573541-7573560	None:intergenic	45.0%
	AATACCGCCAATGCATTTGC+TGG	-	chr4.4:7573915-7573934	None:intergenic	45.0%
	ACCAACAGCTTCTGTGTGAA+TGG	-	chr4.4:7572734-7572753	None:intergenic	45.0%
	ATTCACACAGAAGCTGTTGG+TGG	+	chr4.4:7572733-7572752	MS.gene49741:CDS	45.0%
	CCAACAACTCGAGATCCAAT+TGG	-	chr4.4:7573391-7573410	None:intergenic	45.0%
	CCCTGCTCAAGCCAAAATTA+GGG	+	chr4.4:7574951-7574970	MS.gene49741:intron	45.0%
	CCTGCTCAAGCCAAAATTAG+GGG	+	chr4.4:7574952-7574971	MS.gene49741:intron	45.0%
	CGAAGGAAAAATAGTCGGTC+GGG	+	chr4.4:7575441-7575460	MS.gene49741:CDS	45.0%
	CTGTCTACAAACGGTATGCA+GGG	+	chr4.4:7573515-7573534	MS.gene49741:intron	45.0%
	GAAACAGGTATCTTCGACCT+TGG	+	chr4.4:7575358-7575377	MS.gene49741:CDS	45.0%
	GATGAGAAGTTCACCAGCTT+TGG	-	chr4.4:7572845-7572864	None:intergenic	45.0%
	GCAGGAAATTACTGGTGGAA+AGG	+	chr4.4:7574588-7574607	MS.gene49741:intron	45.0%
	GGAGGAAAAGCAGTGATGAT+TGG	+	chr4.4:7574682-7574701	MS.gene49741:CDS	45.0%
	TAGTGTGAGTGGGTGATGTT+AGG	-	chr4.4:7574509-7574528	None:intergenic	45.0%
	TATCTGCAGACAACTGGAAG+CGG	-	chr4.4:7574284-7574303	None:intergenic	45.0%
	TCCATTCACACAGAAGCTGT+TGG	+	chr4.4:7572730-7572749	MS.gene49741:CDS	45.0%
	TCTAGCTATCTGCAGACAAC+TGG	-	chr4.4:7574290-7574309	None:intergenic	45.0%
	TGCCAGAACTCTATCTTGTG+CGG	+	chr4.4:7574467-7574486	MS.gene49741:intron	45.0%
	TGTTGTGCCAGCAAATGCAT+TGG	+	chr4.4:7573905-7573924	MS.gene49741:CDS	45.0%
	TTCACGCAGGAAATTACTGG+TGG	+	chr4.4:7574583-7574602	MS.gene49741:intron	45.0%
	TTCTTGTAACAGCATGGCCA+AGG	-	chr4.4:7575378-7575397	None:intergenic	45.0%
!	ACAAGCTGGTTCTTTAGGAG+AGG	+	chr4.4:7574711-7574730	MS.gene49741:CDS	45.0%
!	CCAATTGGATCTCGAGTTGT+TGG	+	chr4.4:7573388-7573407	MS.gene49741:CDS	45.0%
!	CGTATAAGGTTCCTTTTGCG+CGG	-	chr4.4:7573577-7573596	None:intergenic	45.0%
!	CTGCAGATAGCTAGAGCATT+TGG	+	chr4.4:7574295-7574314	MS.gene49741:CDS	45.0%
!	GAAACTCGACTGTTTCTTCG+AGG	+	chr4.4:7573602-7573621	MS.gene49741:CDS	45.0%
!	GATGTTGCTGTTGAAGCCTT+AGG	+	chr4.4:7574616-7574635	MS.gene49741:CDS	45.0%
!	GTTGCTGTTATTGGAACTGG+GGG	+	chr4.4:7574038-7574057	MS.gene49741:CDS	45.0%
!!	TATTGGCTCATATGGAGGGA+GGG	+	chr4.4:7575300-7575319	MS.gene49741:CDS	45.0%
!!	TTATTGGCTCATATGGAGGG+AGG	+	chr4.4:7575299-7575318	MS.gene49741:CDS	45.0%
!!!	ACATGCGTGGTGCTGTTTTT+CGG	+	chr4.4:7572773-7572792	MS.gene49741:CDS	45.0%
!!!	CCCTAATTTTGGCTTGAGCA+GGG	-	chr4.4:7574954-7574973	None:intergenic	45.0%
	ACAGCACCACGCATGTAAGA+TGG	-	chr4.4:7572769-7572788	None:intergenic	50.0%
	ATCTCATGGCCCACAACACA+AGG	-	chr4.4:7573149-7573168	None:intergenic	50.0%
	CATATTCCTCGTCCCAAAGC+TGG	+	chr4.4:7572829-7572848	MS.gene49741:CDS	50.0%
	GCATTCCAGGATCTCAACGA+AGG	+	chr4.4:7575424-7575443	MS.gene49741:CDS	50.0%
	GCCCTGCTCAAGCCAAAATT+AGG	+	chr4.4:7574950-7574969	MS.gene49741:intron	50.0%
	GCTGTCTACAAACGGTATGC+AGG	+	chr4.4:7573514-7573533	MS.gene49741:intron	50.0%
	GGAGCCTTCATTATGCCTTG+CGG	+	chr4.4:7573409-7573428	MS.gene49741:CDS	50.0%
	GGATTGCCATCTTACATGCG+TGG	+	chr4.4:7572760-7572779	MS.gene49741:CDS	50.0%
	GTGCACTGAGCAAATGTCTG+TGG	-	chr4.4:7574646-7574665	None:intergenic	50.0%
	GTTGATCAGAGCCTACTGCT+CGG	-	chr4.4:7574233-7574252	None:intergenic	50.0%
	GTTGCCGCAAGGCATAATGA+AGG	-	chr4.4:7573416-7573435	None:intergenic	50.0%
	GTTGTGGGCCATGAGATTAC+TGG	+	chr4.4:7573152-7573171	MS.gene49741:CDS	50.0%
	TGGCATCTGCACTAGTTCGT+AGG	+	chr4.4:7572608-7572627	MS.gene49741:CDS	50.0%
	TGTGCCAGCAAATGCATTGG+CGG	+	chr4.4:7573908-7573927	MS.gene49741:CDS	50.0%
!	AGCTGGTTCTTTAGGAGAGG+TGG	+	chr4.4:7574714-7574733	MS.gene49741:CDS	50.0%
!	CGACTGTTTCTTCGAGGAAG+TGG	+	chr4.4:7573608-7573627	MS.gene49741:CDS	50.0%
!	CTGGCACAAGCTGGTTCTTT+AGG	+	chr4.4:7574706-7574725	MS.gene49741:CDS	50.0%
!	GTTATTGGAACTGGGGGTGT+TGG	+	chr4.4:7574044-7574063	MS.gene49741:CDS	50.0%
!!	ACAGAAGCTGTTGGTGGTGT+TGG	+	chr4.4:7572739-7572758	MS.gene49741:CDS	50.0%
!!	AGCTAGAGCATTTGGTGCCT+CGG	+	chr4.4:7574303-7574322	MS.gene49741:CDS	50.0%
!!	ATGATTGGACTGGCACAAGC+TGG	+	chr4.4:7574697-7574716	MS.gene49741:CDS	50.0%
!!!	CATGCGTGGTGCTGTTTTTC+GGG	+	chr4.4:7572774-7572793	MS.gene49741:CDS	50.0%
!!!	CCCCTAATTTTGGCTTGAGC+AGG	-	chr4.4:7574955-7574974	None:intergenic	50.0%
	AGTATCACCAGGGCGCACTT+CGG	-	chr4.4:7574018-7574037	None:intergenic	55.0%
	CAGTAGGAACAGTTGCCGCA+AGG	-	chr4.4:7573427-7573446	None:intergenic	55.0%
	GGCCGCACAAGATAGAGTTC+TGG	-	chr4.4:7574472-7574491	None:intergenic	55.0%
!	GGAGTCAGGCAAAGCATTCC+AGG	+	chr4.4:7575411-7575430	MS.gene49741:CDS	55.0%
!!	AGTTCACCAGCTTTGGGACG+AGG	-	chr4.4:7572838-7572857	None:intergenic	55.0%
!!	GAACTGGGGGTGTTGGTTCT+AGG	+	chr4.4:7574051-7574070	MS.gene49741:CDS	55.0%
	GCTCATATGGAGGGAGGGCT+AGG	+	chr4.4:7575305-7575324	MS.gene49741:CDS	60.0%
	CATGCAGCCGAAGTGCGCCC+TGG	+	chr4.4:7574008-7574027	MS.gene49741:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	7572607	7575483	7572607	ID=MS.gene49741
chr4.4	mRNA	7572607	7575483	7572607	ID=MS.gene49741.t1;Parent=MS.gene49741
chr4.4	exon	7572607	7572874	7572607	ID=MS.gene49741.t1.exon1;Parent=MS.gene49741.t1
chr4.4	CDS	7572607	7572874	7572607	ID=cds.MS.gene49741.t1;Parent=MS.gene49741.t1
chr4.4	exon	7573087	7573222	7573087	ID=MS.gene49741.t1.exon2;Parent=MS.gene49741.t1
chr4.4	CDS	7573087	7573222	7573087	ID=cds.MS.gene49741.t1;Parent=MS.gene49741.t1
chr4.4	exon	7573384	7573453	7573384	ID=MS.gene49741.t1.exon3;Parent=MS.gene49741.t1
chr4.4	CDS	7573384	7573453	7573384	ID=cds.MS.gene49741.t1;Parent=MS.gene49741.t1
chr4.4	exon	7573536	7573629	7573536	ID=MS.gene49741.t1.exon4;Parent=MS.gene49741.t1
chr4.4	CDS	7573536	7573629	7573536	ID=cds.MS.gene49741.t1;Parent=MS.gene49741.t1
chr4.4	exon	7573859	7574072	7573859	ID=MS.gene49741.t1.exon5;Parent=MS.gene49741.t1
chr4.4	CDS	7573859	7574072	7573859	ID=cds.MS.gene49741.t1;Parent=MS.gene49741.t1
chr4.4	exon	7574291	7574432	7574291	ID=MS.gene49741.t1.exon6;Parent=MS.gene49741.t1
chr4.4	CDS	7574291	7574432	7574291	ID=cds.MS.gene49741.t1;Parent=MS.gene49741.t1
chr4.4	exon	7574592	7574762	7574592	ID=MS.gene49741.t1.exon7;Parent=MS.gene49741.t1
chr4.4	CDS	7574592	7574762	7574592	ID=cds.MS.gene49741.t1;Parent=MS.gene49741.t1
chr4.4	exon	7575292	7575483	7575292	ID=MS.gene49741.t1.exon8;Parent=MS.gene49741.t1
chr4.4	CDS	7575292	7575483	7575292	ID=cds.MS.gene49741.t1;Parent=MS.gene49741.t1

Gene Sequence

>MS.gene49741

ATGGCATCTGCACTAGTTCGTAGGAACATCATCAGCAACAACTTCTTGAAAAGGTTGTCTTGTTGGCGTTTGAATAATAACAAACAGTTGTTGAATAATGTTGAAGCTGATGCATGTACGGTTTCCATTCACACAGAAGCTGTTGGTGGTGTTGGATTGCCATCTTACATGCGTGGTGCTGTTTTTCGGGAACCCAACAAACCTCTCACCATTGAAGAATTTCATATTCCTCGTCCCAAAGCTGGTGAACTTCTCATCAAAACCAAGGGTATTGTTTATCTCTCTTGATGCTATATATATATATATTCTATTCATTTTTTAAATTAAAAATAATGACGATATGTTTCTGATCCTAAAACTTGTATATTGCAGCTTAATTGTGGTTGTGGATTGTAATTCAAAATTATTATTTTTGATATTGTTATGATTGGTGGTTATTATTGGATCTGATTGTGTAAAGATGTGATTTTTTGAATGCAGGATGTGGAGTATGCCACTCTGATCTTCATGTCATGAAAGGTGAAATTCCGTTTTCAAGTCCTTGTGTTGTGGGCCATGAGATTACTGGTGAAGTTGTTGAACATGGACAACACACTGATTCCAAAACCATAGAAAGGTTAAAGTTTTTTTTTTTCTTTGTGTAATTGAAAACTGTCAAACTGCCACATTTTTGTGTGATAATGCTATGATGATGAGTTTGCATTCAATGAAAAGATTGATTGTTCTTGATGACTAGCAAAAAGGAATAACAAGTAGGATTGTTCTTGATTGTTTCAGGCTTCCAATTGGATCTCGAGTTGTTGGAGCCTTCATTATGCCTTGCGGCAACTGTTCCTACTGTTCAAAGGTAACATTGTATTACACACTCTACTCCATTTGTCAACTGAATTATGGTCTCGGTGTTTATGCTGTCTACAAACGGTATGCAGGGCCATGATGATCTGTGCGAAGCTTTTTTTGCTTATAACCGCGCAAAAGGAACCTTATACGATGGTGAAACTCGACTGTTTCTTCGAGGAAGTGGTGAACCCTCTTACTTCTTACTTGATTCACATATTACCGATACATGTGAAATGATTGTATGTTATCATTATTCCCATATAGACAAGCTTAATTTATACCTAAGTATGTGTTTACATAGTCATAGTTTTCTCTGCTATGGTATTTCATATTCAGCTGATACAAATTTCATATTTGTTGAGAGTAGACTCCTTTATAATTGGAATACTGAAAATAATTGGCAAATTTATAGGAAAACCGATATACATGTATAGTATGGGAGGGCTTGCTGAATACTGTGTTGTGCCAGCAAATGCATTGGCGGTATTGCCAAACTCAATGCCATATACCGAGTCTGCAATTCTAGGATGTGCTGTTTTTACTGCTTATGGTGCAATGGCTCATGCAGCCGAAGTGCGCCCTGGTGATACTGTTGCTGTTATTGGAACTGGGGGTGTTGGTTCTAGGTAACTAAATCAATTGTCAAGTGGATGTCACATTGTTAAATATTTAATCATCAGTGACCACTTCCTTTTTCTATTTTTGCTGTTTCGATAATGCTTGCTTAAATGGTCACTGATTTGAGAAGAAAACTCTTTATATTATGCAAAACTTTTCATATATCCGAGCAGTAGGCTCTGATCAACTGCACTTGTTTCCATTTTAACTTTATCACCGCTTCCAGTTGTCTGCAGATAGCTAGAGCATTTGGTGCCTCGGATATAATTGCTGTTGATGTTCAGGATGAAAAGCTAGAGAAAGCAAAGACACTTGGTGCAACTCACACAATAAATTCGGCTAAGGAAGATCCCATTGAAAAGATACTTGTATATGCATTTCTCTTTCGCCTCATTGTTTAATTGCCAGAACTCTATCTTGTGCGGCCTCTGTCTATTAGCTCCTAACATCACCCACTCACACTATCGTGCATAAATTATATCGCTTGAGAGATCCTTTATTTAATTGTTTTCTCTAACTTCACGCAGGAAATTACTGGTGGAAAGGGTGTTGATGTTGCTGTTGAAGCCTTAGGGAGACCACAGACATTTGCTCAGTGCACACAAAGTGTGAAAGATGGAGGAAAAGCAGTGATGATTGGACTGGCACAAGCTGGTTCTTTAGGAGAGGTGGATATAAATAGACTTGTTCGCAGAAAGGTAAGTGCTTTGTGTGTTGTATACATTGTCGATCGTTACTATAATTACTTTTTCTACTCTGACATATAACCACTAGTTTTATTCATGTTGGATTTAAACTGTTTAGCACACTTATCGTTATTAATGTTTGTTTTGTTCTAGAATTTGCTTCATTTTTTTCTGCGATTTATCGCAATGTCTTAATCTTGCCCTGCTCAAGCCAAAATTAGGGGTATGGGAATAAGAATTAGGTTGTACAAGTGGTTTTGCCTTTACTTGAACTGTTGAACAAATGATTCAAGATAACCTTTGGGATTAAATTGTTCATATCAAGGGCCGCTTTTAAACTCAATTGAGTGTTACATGTGAACAATATAGAGACTATAAATATGTAATATTTATTGATGATGCTAATTTTCTACCAAAGCAGAATTGACGTGAAGTGCTAAAACATAAAGTATACATTACTAAATGACAATAATTTCCTTTAGTGTAGCACCTCACTTATTTGACAAATTCATCCAAGCAAATTAAATTTGTATGTGAACAGATAAAAGTTATTGGCTCATATGGAGGGAGGGCTAGGCAAGATCTTCCAAAGTTAATTAGATTGGCAGAAACAGGTATCTTCGACCTTGGCCATGCTGTTACAAGAAAATACACTTTTGAGGAGTCAGGCAAAGCATTCCAGGATCTCAACGAAGGAAAAATAGTCGGTCGGGCTGTAATCGAGATAGTATAG

Protein sequence

>MS.gene49741.t1

MASALVRRNIISNNFLKRLSCWRLNNNKQLLNNVEADACTVSIHTEAVGGVGLPSYMRGAVFREPNKPLTIEEFHIPRPKAGELLIKTKGCGVCHSDLHVMKGEIPFSSPCVVGHEITGEVVEHGQHTDSKTIERLPIGSRVVGAFIMPCGNCSYCSKGHDDLCEAFFAYNRAKGTLYDGETRLFLRGSGKPIYMYSMGGLAEYCVVPANALAVLPNSMPYTESAILGCAVFTAYGAMAHAAEVRPGDTVAVIGTGGVGSSCLQIARAFGASDIIAVDVQDEKLEKAKTLGATHTINSAKEDPIEKILEITGGKGVDVAVEALGRPQTFAQCTQSVKDGGKAVMIGLAQAGSLGEVDINRLVRRKIKVIGSYGGRARQDLPKLIRLAETGIFDLGHAVTRKYTFEESGKAFQDLNEGKIVGRAVIEIV