AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86920.t1 MTR_1g106860 97.567 781 19 0 1 781 1 781 0.0 1579
MS.gene86920.t1 MTR_2g008240 56.619 763 310 10 5 759 16 765 0.0 867
MS.gene86920.t1 MTR_4g094285 55.150 767 316 11 6 759 13 764 0.0 857
MS.gene86920.t1 MTR_1g087240 54.354 758 333 6 3 759 2 747 0.0 846
MS.gene86920.t1 MTR_7g109390 53.940 736 327 6 25 759 29 753 0.0 827
MS.gene86920.t1 MTR_2g034720 52.477 747 339 5 25 771 28 758 0.0 810
MS.gene86920.t1 MTR_5g062430 49.486 778 363 14 4 759 3 772 0.0 766
MS.gene86920.t1 MTR_3g074060 50.067 749 347 11 25 759 31 766 0.0 757
MS.gene86920.t1 MTR_5g062320 49.671 759 360 13 24 773 34 779 0.0 756
MS.gene86920.t1 MTR_5g030860 49.352 772 363 10 8 759 9 772 0.0 754
MS.gene86920.t1 MTR_5g062370 49.022 767 370 14 5 759 15 772 0.0 751
MS.gene86920.t1 MTR_5g062650 48.181 797 371 16 4 773 3 784 0.0 741
MS.gene86920.t1 MTR_5g062370 52.677 579 258 10 5 573 15 587 0.0 622
MS.gene86920.t1 MTR_6g028120 26.119 670 349 29 32 625 3 602 1.04e-36 147
MS.gene86920.t1 MTR_7g086030 26.873 614 351 27 38 591 4 579 3.45e-35 142
MS.gene86920.t1 MTR_7g086030 26.873 614 351 27 38 591 29 604 5.89e-35 142
MS.gene86920.t1 MTR_6g028090 26.626 661 327 32 47 625 37 621 3.40e-34 139
MS.gene86920.t1 MTR_6g028100 26.101 613 339 25 47 591 37 603 8.59e-33 135
MS.gene86920.t1 MTR_3g079750 25.943 636 361 29 48 625 38 621 1.37e-32 134
MS.gene86920.t1 MTR_6g028110 25.446 617 338 25 47 591 38 604 4.21e-32 133
MS.gene86920.t1 MTR_6g028110 24.408 676 373 27 26 629 17 626 5.48e-32 132
MS.gene86920.t1 MTR_2g030000 26.052 618 331 27 48 591 40 605 9.34e-30 126
MS.gene86920.t1 MTR_5g069800 25.421 535 327 23 73 581 85 573 1.30e-26 115
MS.gene86920.t1 MTR_3g462820 24.225 677 390 25 11 626 3 617 1.53e-26 115
MS.gene86920.t1 MTR_5g069740 24.953 529 325 22 73 581 85 561 5.92e-25 110
MS.gene86920.t1 MTR_7g086010 28.338 367 215 17 73 423 118 452 1.18e-23 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86920.t1 AT3G19620 70.658 760 204 8 21 772 23 771 0.0 1114
MS.gene86920.t1 AT5G64570 56.364 770 305 13 4 759 22 774 0.0 867
MS.gene86920.t1 AT5G09730 53.544 790 328 15 2 773 4 772 0.0 835
MS.gene86920.t1 AT1G02640 53.117 770 333 8 3 763 4 754 0.0 829
MS.gene86920.t1 AT5G49360 53.264 766 338 10 1 759 10 762 0.0 810
MS.gene86920.t1 AT5G10560 48.545 756 354 8 31 759 35 782 0.0 746
MS.gene86920.t1 AT1G78060 48.477 755 375 9 14 761 12 759 0.0 735
MS.gene86920.t1 AT5G64570 61.140 579 215 7 4 578 22 594 0.0 724
MS.gene86920.t1 AT5G64570 56.398 633 248 11 138 759 1 616 0.0 715
MS.gene86920.t1 AT5G09700 49.482 483 226 7 286 760 45 517 1.16e-157 469
MS.gene86920.t1 AT3G47040 25.723 657 363 30 38 628 10 607 1.11e-32 134
MS.gene86920.t1 AT3G47010 25.920 625 371 25 32 600 5 593 1.18e-32 134
MS.gene86920.t1 AT3G47000 25.460 652 365 26 38 625 10 604 1.47e-32 134
MS.gene86920.t1 AT3G62710 27.036 614 348 31 45 624 101 648 4.13e-32 133
MS.gene86920.t1 AT3G47010 25.080 622 379 23 32 600 5 592 1.28e-30 128
MS.gene86920.t1 AT5G20950 25.466 644 353 28 48 625 114 696 1.83e-30 128
MS.gene86920.t1 AT5G20950 25.309 648 357 28 48 629 36 622 1.86e-30 128
MS.gene86920.t1 AT5G20950 25.309 648 357 28 48 629 36 622 1.86e-30 128
MS.gene86920.t1 AT3G47050 25.373 603 379 22 35 591 7 584 1.49e-29 125
MS.gene86920.t1 AT5G04885 26.497 551 322 24 72 592 125 622 2.36e-29 125
MS.gene86920.t1 AT5G04885 26.497 551 322 24 72 592 108 605 2.80e-29 124
MS.gene86920.t1 AT5G04885 26.497 551 322 24 72 592 108 605 2.80e-29 124
MS.gene86920.t1 AT3G47040 25.037 683 360 32 38 628 10 632 3.69e-29 124
MS.gene86920.t1 AT3G47000 28.000 450 226 18 38 423 10 425 1.79e-27 117
MS.gene86920.t1 AT3G47040 27.169 438 245 18 38 423 10 425 2.97e-26 113
MS.gene86920.t1 AT5G20940 26.514 611 334 28 48 591 42 604 1.26e-25 113
MS.gene86920.t1 AT3G47010 27.679 448 242 19 32 423 5 426 6.93e-25 108
MS.gene86920.t1 AT3G47010 25.240 416 270 16 199 600 26 414 1.31e-20 95.9
MS.gene86920.t1 AT3G47000 24.459 462 291 20 183 625 5 427 5.05e-18 87.8
MS.gene86920.t1 AT3G47050 25.600 375 221 13 35 365 7 367 1.30e-17 86.3

Find 169 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGGCTGGTTTGACATTT+TGG 0.130488 1.2:-79194607 MS.gene86920:CDS
GCAACATAGGAGGGATATTA+TGG 0.220056 1.2:-79192684 MS.gene86920:CDS
TGAAACATTATGGTACAAAA+TGG 0.221947 1.2:-79194672 MS.gene86920:CDS
CTGCTTATGATGTTGATAAT+TGG 0.227264 1.2:-79194403 MS.gene86920:CDS
GCCATAGCACAAGTAATATT+TGG 0.228912 1.2:-79192631 MS.gene86920:CDS
GGGAAATCTCTCTATGAATT+TGG 0.245260 1.2:-79191892 MS.gene86920:CDS
GTGTCATGTGCTCATATAAT+AGG 0.254476 1.2:-79194103 MS.gene86920:CDS
GATTGTGGATACCATGTAAA+TGG 0.264876 1.2:+79192003 None:intergenic
TCCAACACACAGCTCTTAAA+TGG 0.278527 1.2:+79194140 None:intergenic
TTATTACCTTAGCATCAAAA+TGG 0.295146 1.2:+79194363 None:intergenic
CAAAGAAGCCAAGTCTCATT+AGG 0.298010 1.2:+79193247 None:intergenic
TGTCAAATTCAAACAACTTA+TGG 0.308883 1.2:+79191735 None:intergenic
GCCCTTCTGCTTCTATAGAT+TGG 0.325933 1.2:+79192842 None:intergenic
TTTATCTTCGTCTTTGATAA+AGG 0.334170 1.2:+79193096 None:intergenic
GATAGTGAAGTGTTGCAAAA+TGG 0.335511 1.2:+79194923 None:intergenic
AGGGAAATTTCTACTGCTAT+TGG 0.339643 1.2:+79191939 None:intergenic
AGCCTTGAAATGCCGGTAGA+AGG 0.350533 1.2:+79194830 None:intergenic
GATTGGACAGCACACTATTC+TGG 0.361517 1.2:-79191482 MS.gene86920:CDS
AGAGGCCAGTGGGGTCTAGA+TGG 0.363411 1.2:-79194029 MS.gene86920:CDS
GCAAGAGCCACTGCATCTTC+AGG 0.368484 1.2:+79193503 None:intergenic
GACTAAAATCAATGTTCCTT+TGG 0.370514 1.2:+79192753 None:intergenic
TTACAAATGGAGAAGCATTA+AGG 0.375714 1.2:-79191417 MS.gene86920:CDS
AGCTATGTACAATGTGGATT+TGG 0.386393 1.2:-79194624 MS.gene86920:CDS
AGAGTTAACTTGACATTGCC+TGG 0.387425 1.2:-79192814 MS.gene86920:CDS
TTAGTGAAAGAAATATCAAT+TGG 0.388314 1.2:+79192715 None:intergenic
GATGCAGTGGTGTTGGTTGT+TGG 0.395186 1.2:-79192871 MS.gene86920:CDS
TTTGCAAATGGAAGTGACTT+TGG 0.397416 1.2:+79193219 None:intergenic
GTTCCACTGAAACAATTGAT+AGG 0.400095 1.2:-79191613 MS.gene86920:CDS
TTGCAAATGGAAGTGACTTT+GGG 0.402569 1.2:+79193220 None:intergenic
AACTCCAGGGGAAGATCCTT+TGG 0.404077 1.2:-79194534 MS.gene86920:CDS
TGCTAAAGATGATAGGCTTA+AGG 0.408182 1.2:-79194450 MS.gene86920:CDS
ACTGTGGTGGCATTTGCATT+TGG 0.412568 1.2:+79193054 None:intergenic
CCACTCACCACCTCTGAACT+TGG 0.416144 1.2:+79191637 None:intergenic
CTCATGTTCATGTCTGTCAT+TGG 0.420175 1.2:+79191964 None:intergenic
ACAAGCTTCTCTTGAAATCC+AGG 0.424917 1.2:+79192796 None:intergenic
TCTACCGGCATTTCAAGGCT+TGG 0.426574 1.2:-79194827 MS.gene86920:CDS
CTCTGCTGATGCAGTGGTGT+TGG 0.432899 1.2:-79192878 MS.gene86920:CDS
ACCCTTCTACCGGCATTTCA+AGG 0.433066 1.2:-79194832 MS.gene86920:CDS
ACTTCTGTAGTCCTTGTAAA+GGG 0.438650 1.2:+79192989 None:intergenic
TACTTCTGTAGTCCTTGTAA+AGG 0.441252 1.2:+79192988 None:intergenic
AAAGAGCACAAGTTAAGGTT+GGG 0.443226 1.2:-79191585 MS.gene86920:CDS
CACCACATTGATATTACAAA+TGG 0.443706 1.2:-79191430 MS.gene86920:CDS
GTTGCAAAATGGAAAGTTGC+TGG 0.448852 1.2:+79194934 None:intergenic
CACTAAAGAAAATCAGCAAT+TGG 0.455452 1.2:-79193175 MS.gene86920:CDS
ATTTGAAAGAGCACAAGTTA+AGG 0.457325 1.2:-79191590 MS.gene86920:CDS
GAAAGAGCACAAGTTAAGGT+TGG 0.460343 1.2:-79191586 MS.gene86920:CDS
AAGCAAGTGCTAAAGATGAT+AGG 0.460961 1.2:-79194457 MS.gene86920:CDS
TGCTCATATAATAGGGTGAA+TGG 0.462881 1.2:-79194095 MS.gene86920:CDS
TTGGTGTTCCTGCTTATGAA+TGG 0.463212 1.2:-79194808 MS.gene86920:CDS
ATTACACCTTGGAGAAGGTC+TGG 0.463689 1.2:+79194053 None:intergenic
GAAACATTATGGTACAAAAT+GGG 0.466706 1.2:-79194671 MS.gene86920:CDS
AGTGGTGTTGGTTGTTGGAT+TGG 0.468220 1.2:-79192866 MS.gene86920:CDS
GGTAAAAGAAAGTTGGTGAT+TGG 0.468406 1.2:-79191499 MS.gene86920:CDS
ATCTATAGAAGCAGAAGGGC+TGG 0.468944 1.2:-79192839 MS.gene86920:CDS
ATTTCCCTGGTAGAACATAC+AGG 0.469611 1.2:-79191924 MS.gene86920:CDS
AATCCTATCAATTGTTTCAG+TGG 0.470201 1.2:+79191610 None:intergenic
TGTCATGTGCTCATATAATA+GGG 0.473992 1.2:-79194102 MS.gene86920:CDS
CAGGGACGCGTTTCGATAGC+AGG 0.477482 1.2:-79194748 MS.gene86920:CDS
ATTTAAGAGCTGTGTGTTGG+AGG 0.484967 1.2:-79194138 MS.gene86920:CDS
ACTAAAAGTGTAAGCAACAT+AGG 0.484978 1.2:-79192697 MS.gene86920:CDS
TGACACTAAGTCCTTGGCTC+TGG 0.485207 1.2:+79194892 None:intergenic
TCACTTCCATTTGCAAACCT+TGG 0.486006 1.2:-79193213 MS.gene86920:CDS
TCTCTCTATGAATTTGGCTA+TGG 0.491259 1.2:-79191886 MS.gene86920:CDS
AACACCAAGCCTTGAAATGC+CGG 0.491966 1.2:+79194823 None:intergenic
TGAATATCGGCATGGTATGC+CGG 0.492312 1.2:+79193014 None:intergenic
TTCTGGATCATTATGGTGGA+AGG 0.493551 1.2:+79191823 None:intergenic
TATGCTGTAAATTATGTTCG+TGG 0.495123 1.2:-79194500 MS.gene86920:CDS
CAACATAGGAGGGATATTAT+GGG 0.496768 1.2:-79192683 MS.gene86920:CDS
ATTGTGGATACCATGTAAAT+GGG 0.498218 1.2:+79192004 None:intergenic
CGATATTCAAGCCCTTTACA+AGG 0.501394 1.2:-79193000 MS.gene86920:CDS
TCCAAATATTACTTGTGCTA+TGG 0.501814 1.2:+79192630 None:intergenic
TAGTGAAAGAAATATCAATT+GGG 0.504026 1.2:+79192716 None:intergenic
TCCATTTAAGAGCTGTGTGT+TGG 0.505176 1.2:-79194141 MS.gene86920:CDS
ATGTACAATGTGGATTTGGC+TGG 0.507340 1.2:-79194620 MS.gene86920:CDS
GATGGTCCAAGGTTTGCAAA+TGG 0.509359 1.2:+79193207 None:intergenic
GCCTTGAAATGCCGGTAGAA+GGG 0.510160 1.2:+79194831 None:intergenic
ATATTATGGGTAGGTTATCC+TGG 0.512772 1.2:-79192670 MS.gene86920:CDS
CTTGTAAAGGGCTTGAATAT+CGG 0.513958 1.2:+79193001 None:intergenic
ATATTTGGAGACTATAACCC+AGG 0.518089 1.2:-79192616 MS.gene86920:intron
CTGCTATCGAAACGCGTCCC+TGG 0.518333 1.2:+79194749 None:intergenic
GCAGGTGACAAAGCAAGACT+TGG 0.520570 1.2:-79194180 MS.gene86920:intron
TCTTTAGTGCATACATCAGA+TGG 0.520982 1.2:+79193189 None:intergenic
TGGTGGTCAGAGGCACTTCA+TGG 0.521560 1.2:-79194788 MS.gene86920:CDS
GGTGACAAAGCAAGACTTGG+AGG 0.523632 1.2:-79194177 MS.gene86920:CDS
CGTTTCGATAGCAGGGTACC+TGG 0.524275 1.2:-79194740 MS.gene86920:CDS
TATCAATTGCTTGATCATCA+AGG 0.526561 1.2:+79191764 None:intergenic
GTGGCTCTTGCACTGAAAGC+AGG 0.529634 1.2:-79193491 MS.gene86920:intron
GGCAGCCTCTGCTGATGCAG+TGG 0.534510 1.2:-79192884 MS.gene86920:CDS
CTTCATGGTGTCTCAAATGT+AGG 0.539417 1.2:-79194773 MS.gene86920:CDS
GGGCGTGGACAAGAAACTCC+AGG 0.541036 1.2:-79194548 MS.gene86920:CDS
TTTAAGAGCTGTGTGTTGGA+GGG 0.541333 1.2:-79194137 MS.gene86920:CDS
GTTCGTGGACTACAAGAAGT+TGG 0.541602 1.2:-79194485 MS.gene86920:CDS
ACCACATGAGATCCGTCAAA+AGG 0.541648 1.2:+79191679 None:intergenic
TAAAACCTGTATGTTCTACC+AGG 0.542758 1.2:+79191919 None:intergenic
ATAATTGGAAAGGCGTTGAT+CGG 0.552315 1.2:-79194388 MS.gene86920:CDS
GGACAGCACACTATTCTGGT+TGG 0.552636 1.2:-79191478 MS.gene86920:CDS
TTTACTAATTAGATTCACTC+AGG 0.554229 1.2:+79191394 None:intergenic
TAAGCGTGACACTAAGTCCT+TGG 0.559465 1.2:+79194886 None:intergenic
TGCTATGTGGGTGGAGAATG+TGG 0.559491 1.2:+79191852 None:intergenic
CTCTGATTACACCTTGGAGA+AGG 0.562519 1.2:+79194048 None:intergenic
ATCTATTGTAGACCAAGCCT+TGG 0.563804 1.2:-79193286 MS.gene86920:CDS
GAGGCCAGTGGGGTCTAGAT+GGG 0.564760 1.2:-79194028 MS.gene86920:intron
GCAGCTGATAGTATCACTGC+TGG 0.566392 1.2:+79194704 None:intergenic
CTCACCACCTCTGAACTTGG+TGG 0.569744 1.2:+79191640 None:intergenic
TCTGACCACCATTCATAAGC+AGG 0.570631 1.2:+79194800 None:intergenic
TGTTTCAGACTGTGATTCAG+TGG 0.570986 1.2:-79193561 MS.gene86920:CDS
GCTCTAGAAGCTGCGAAGCA+AGG 0.571329 1.2:-79193153 MS.gene86920:CDS
GGTGTCTCAAATGTAGGACC+AGG 0.572126 1.2:-79194767 MS.gene86920:CDS
GTGTTCCTGCTTATGAATGG+TGG 0.572399 1.2:-79194805 MS.gene86920:CDS
TGCATACATCAGATGGTCCA+AGG 0.572554 1.2:+79193196 None:intergenic
AAAAGTGTAAGCAACATAGG+AGG 0.573233 1.2:-79192694 MS.gene86920:CDS
AGGTGTAATCAGAGGCCAGT+GGG 0.573573 1.2:-79194039 MS.gene86920:CDS
GTAGGTTATCCTGGTCAAGA+TGG 0.574821 1.2:-79192661 MS.gene86920:CDS
TACATAGTCCTAATGAGACT+TGG 0.576502 1.2:-79193255 MS.gene86920:CDS
GGAAGGAGCAGATGCTATGT+GGG 0.581485 1.2:+79191840 None:intergenic
TTTGGAGCCACCAAGTTCAG+AGG 0.581797 1.2:-79191647 MS.gene86920:CDS
CAAATTCAAACAACTTATGG+TGG 0.582768 1.2:+79191738 None:intergenic
GTTATGATTAGTAACTATGC+CGG 0.583450 1.2:-79193033 MS.gene86920:CDS
TGTAGTTGTATACCAAGGCT+TGG 0.586966 1.2:+79193274 None:intergenic
GCATTTCCTCCATCTTGACC+AGG 0.588584 1.2:+79192652 None:intergenic
GTGGTAGGTCCCCATTTACA+TGG 0.594929 1.2:-79192014 MS.gene86920:CDS
CAACTAGTAAACCCTTCTAC+CGG 0.596049 1.2:-79194842 MS.gene86920:CDS
ACAACCAAAGGATCTTCCCC+TGG 0.596108 1.2:+79194530 None:intergenic
TAGTGATGGTAAAAGAAAGT+TGG 0.596133 1.2:-79191506 MS.gene86920:CDS
GGCGTGGACAAGAAACTCCA+GGG 0.596923 1.2:-79194547 MS.gene86920:CDS
AATAGCAGTAGAAATTTCCC+TGG 0.600435 1.2:-79191937 MS.gene86920:CDS
GACCAATCTATAGAAGCAGA+AGG 0.600841 1.2:-79192844 MS.gene86920:CDS
CTAAGTAATGTTGATAGTGA+TGG 0.602583 1.2:-79191520 MS.gene86920:CDS
GGAGCCACCAAGTTCAGAGG+TGG 0.603381 1.2:-79191644 MS.gene86920:CDS
TGCTTATGAATGGTGGTCAG+AGG 0.605760 1.2:-79194798 MS.gene86920:CDS
ATCTTATGAAACCAGAGCCA+AGG 0.606136 1.2:-79194903 MS.gene86920:CDS
TCTTCAGTAACTTACGCGCA+TGG 0.611081 1.2:-79192961 MS.gene86920:CDS
AAAGTGTAAGCAACATAGGA+GGG 0.615065 1.2:-79192693 MS.gene86920:CDS
TATGATGTTGATAATTGGAA+AGG 0.616039 1.2:-79194398 MS.gene86920:CDS
AGGTCTGGATCAGCACATGT+AGG 0.621412 1.2:+79194068 None:intergenic
AAAGACTCCTGAAGATGCAG+TGG 0.622680 1.2:-79193510 MS.gene86920:CDS
GTGATTGGTGTGAAGAACAA+TGG 0.624345 1.2:-79191703 MS.gene86920:CDS
GATGTGGCTGCTGCTACCAA+AGG 0.627255 1.2:-79192769 MS.gene86920:CDS
CCAAGTTCAGAGGTGGTGAG+TGG 0.628834 1.2:-79191637 MS.gene86920:CDS
GGTTATCCTGGTCAAGATGG+AGG 0.630089 1.2:-79192658 MS.gene86920:CDS
CTCCATTTGTAATATCAATG+TGG 0.634610 1.2:+79191428 None:intergenic
ACTTGATCAACATATGATTG+TGG 0.635008 1.2:+79191988 None:intergenic
GTTACTAATCATAACTGTGG+TGG 0.637990 1.2:+79193041 None:intergenic
ACTGGCCTCTGATTACACCT+TGG 0.640961 1.2:+79194042 None:intergenic
CTTCTCCAAGGTGTAATCAG+AGG 0.642134 1.2:-79194047 MS.gene86920:CDS
TGGAAGGAGCAGATGCTATG+TGG 0.642253 1.2:+79191839 None:intergenic
CAACACCACTGCATCAGCAG+AGG 0.646395 1.2:+79192879 None:intergenic
GTTCTGCTTGAAAACAACAA+AGG 0.647276 1.2:-79193126 MS.gene86920:CDS
CAAACCCATCTAGACCCCAC+TGG 0.647961 1.2:+79194024 None:intergenic
GACTATGTAGTTGTATACCA+AGG 0.652901 1.2:+79193269 None:intergenic
GCTGGAAAGCTAGTAGCACC+AGG 0.654050 1.2:+79194722 None:intergenic
AACACACAGCTCTTAAATGG+AGG 0.654259 1.2:+79194143 None:intergenic
GCTGATCCAGACCTTCTCCA+AGG 0.658385 1.2:-79194059 MS.gene86920:CDS
ATAGGAGGGATATTATGGGT+AGG 0.660257 1.2:-79192679 MS.gene86920:CDS
ACCAATCTATAGAAGCAGAA+GGG 0.660435 1.2:-79192843 MS.gene86920:CDS
AGCTAGAGCTATGTACAATG+TGG 0.670531 1.2:-79194630 MS.gene86920:CDS
AAAACCTGTATGTTCTACCA+GGG 0.670795 1.2:+79191920 None:intergenic
AGGAGCAGATGCTATGTGGG+TGG 0.677137 1.2:+79191843 None:intergenic
AAAGGGCTTGAATATCGGCA+TGG 0.678069 1.2:+79193006 None:intergenic
ATAGTTACTAATCATAACTG+TGG 0.678359 1.2:+79193038 None:intergenic
AGGGACGCGTTTCGATAGCA+GGG 0.694015 1.2:-79194747 MS.gene86920:CDS
AAGGTGTAATCAGAGGCCAG+TGG 0.694576 1.2:-79194040 MS.gene86920:CDS
GCATATCTAGAAACAACCAA+AGG 0.695865 1.2:+79194518 None:intergenic
GCGTGGACAAGAAACTCCAG+GGG 0.698903 1.2:-79194546 MS.gene86920:CDS
GTGTCTCAAATGTAGGACCA+GGG 0.699223 1.2:-79194766 MS.gene86920:CDS
AGAGAAGCTTGTGAAAGATG+TGG 0.705643 1.2:-79192785 MS.gene86920:CDS
GGTGTAATCAGAGGCCAGTG+GGG 0.715668 1.2:-79194038 MS.gene86920:CDS
TTGTGGATACCATGTAAATG+GGG 0.746412 1.2:+79192005 None:intergenic
GCAGGTTTAAACATGAACTG+CGG 0.750864 1.2:-79193363 MS.gene86920:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAAGTTTATGTGTT+TGG + chr1.2:79194325-79194344 None:intergenic 15.0%
!! AAATATGTAAAGATAAATGT+AGG + chr1.2:79193877-79193896 None:intergenic 15.0%
!!! ACAGTGAAAATTTTATTTAT+AGG - chr1.2:79194403-79194422 MS.gene86920:CDS 15.0%
!!! ATTATTAAGTGTTTTTGAAA+TGG + chr1.2:79194650-79194669 None:intergenic 15.0%
!!! GCTATTATAAATTGATAATT+AGG + chr1.2:79194099-79194118 None:intergenic 15.0%
!! AAAAAATCAAATGATTGCTT+AGG + chr1.2:79192449-79192468 None:intergenic 20.0%
!! AAAAATCAAATGATTGCTTA+GGG + chr1.2:79192448-79192467 None:intergenic 20.0%
!! AAAGACGAAGATAAAAAATT+TGG - chr1.2:79193351-79193370 MS.gene86920:CDS 20.0%
!! GTAAAAATATTTAACACGTT+CGG + chr1.2:79192191-79192210 None:intergenic 20.0%
!! TAGTGAAAGAAATATCAATT+GGG + chr1.2:79193726-79193745 None:intergenic 20.0%
!! TATTGGATTATTAAATGGTA+TGG - chr1.2:79193040-79193059 MS.gene86920:CDS 20.0%
!! TCAACTAAATCCTATATTTA+GGG + chr1.2:79192559-79192578 None:intergenic 20.0%
!! TCTATCATAGTATTTATTAC+AGG - chr1.2:79192673-79192692 MS.gene86920:CDS 20.0%
!! TTAGTGAAAGAAATATCAAT+TGG + chr1.2:79193727-79193746 None:intergenic 20.0%
!! TTGACTAAATTACTAGAATT+TGG - chr1.2:79192575-79192594 MS.gene86920:intron 20.0%
!! TTGATTCATAAAAATCAGTT+TGG - chr1.2:79194132-79194151 MS.gene86920:CDS 20.0%
!!! AAATTTTATTTATAGGTGGT+AGG - chr1.2:79194410-79194429 MS.gene86920:CDS 20.0%
!!! AACATTGATTTTAGTCATAA+TGG - chr1.2:79193693-79193712 MS.gene86920:intron 20.0%
!!! AATGTTTTTTTAACTGTCAT+AGG + chr1.2:79193905-79193924 None:intergenic 20.0%
!!! AATTAGTAGTATTGTTTTAC+AGG - chr1.2:79192630-79192649 MS.gene86920:CDS 20.0%
!!! CAAAAAAGTATAAGATATCA+GGG + chr1.2:79192847-79192866 None:intergenic 20.0%
!!! CTAGTTTTAATGAAACATTA+TGG - chr1.2:79191757-79191776 MS.gene86920:CDS 20.0%
!!! GTGAAAATTTTATTTATAGG+TGG - chr1.2:79194406-79194425 MS.gene86920:CDS 20.0%
!!! TCAAAAAAGTATAAGATATC+AGG + chr1.2:79192848-79192867 None:intergenic 20.0%
! AAAAATCAAACGATAACGAA+TGG + chr1.2:79194024-79194043 None:intergenic 25.0%
! AATAAAGTTATGACTGGTAT+TGG - chr1.2:79193023-79193042 MS.gene86920:CDS 25.0%
! ACTGCAAAAAAAATTCTGAT+TGG - chr1.2:79192995-79193014 MS.gene86920:CDS 25.0%
! ATAAAAAATTTGGCTGTGAT+TGG - chr1.2:79193361-79193380 MS.gene86920:CDS 25.0%
! ATAGTTACTAATCATAACTG+TGG + chr1.2:79193404-79193423 None:intergenic 25.0%
! ATTACTAGAATTTGGAAAGT+TGG - chr1.2:79192583-79192602 MS.gene86920:intron 25.0%
! CTGCAAAAAAAATTCTGATT+GGG - chr1.2:79192996-79193015 MS.gene86920:CDS 25.0%
! GAAACATTATGGTACAAAAT+GGG - chr1.2:79191768-79191787 MS.gene86920:CDS 25.0%
! GTCAACTAAATCCTATATTT+AGG + chr1.2:79192560-79192579 None:intergenic 25.0%
! TAGTTTATCATAGCTATCAT+AGG + chr1.2:79192522-79192541 None:intergenic 25.0%
! TATCACCATGTAGATATTTA+CGG - chr1.2:79192228-79192247 MS.gene86920:intron 25.0%
! TGAAACATTATGGTACAAAA+TGG - chr1.2:79191767-79191786 MS.gene86920:CDS 25.0%
! TGTCAAATTCAAACAACTTA+TGG + chr1.2:79194707-79194726 None:intergenic 25.0%
! TTATGTGTTTGGTATAAACT+TGG + chr1.2:79194314-79194333 None:intergenic 25.0%
! TTATTACCTTAGCATCAAAA+TGG + chr1.2:79192079-79192098 None:intergenic 25.0%
! TTTAATAGAAAGCTATACCT+GGG + chr1.2:79193843-79193862 None:intergenic 25.0%
! TTTATCTTCGTCTTTGATAA+AGG + chr1.2:79193346-79193365 None:intergenic 25.0%
! TTTATTACAGGTTGTAGAAA+AGG - chr1.2:79192685-79192704 MS.gene86920:CDS 25.0%
!! ACTGGTATTGGATTATTAAA+TGG - chr1.2:79193035-79193054 MS.gene86920:CDS 25.0%
!! TGTTTATATTTGAGACCATA+AGG + chr1.2:79194264-79194283 None:intergenic 25.0%
!!! TACAATTCGGTTTTATGTAA+AGG - chr1.2:79194165-79194184 MS.gene86920:CDS 25.0%
!!! TATGATGTTGATAATTGGAA+AGG - chr1.2:79192041-79192060 MS.gene86920:intron 25.0%
AACAACTCTAACATTCGATT+TGG - chr1.2:79194046-79194065 MS.gene86920:CDS 30.0%
AATCCTATCAATTGTTTCAG+TGG + chr1.2:79194832-79194851 None:intergenic 30.0%
AATCTCCGTAAATATCTACA+TGG + chr1.2:79192236-79192255 None:intergenic 30.0%
ACGCAGTTGAAAATTTGAAA+CGG - chr1.2:79192749-79192768 MS.gene86920:CDS 30.0%
ACTTGATCAACATATGATTG+TGG + chr1.2:79194454-79194473 None:intergenic 30.0%
ATTGTGGATACCATGTAAAT+GGG + chr1.2:79194438-79194457 None:intergenic 30.0%
CAAATTCAAACAACTTATGG+TGG + chr1.2:79194704-79194723 None:intergenic 30.0%
CACCACATTGATATTACAAA+TGG - chr1.2:79195009-79195028 MS.gene86920:CDS 30.0%
CACTAAAGAAAATCAGCAAT+TGG - chr1.2:79193264-79193283 MS.gene86920:CDS 30.0%
CTATCTTAGTCCCTAAATAT+AGG - chr1.2:79192546-79192565 MS.gene86920:intron 30.0%
CTCCATTTGTAATATCAATG+TGG + chr1.2:79195014-79195033 None:intergenic 30.0%
GAAACGGTGTCTTATAAAAA+GGG - chr1.2:79192765-79192784 MS.gene86920:CDS 30.0%
GACTAAAATCAATGTTCCTT+TGG + chr1.2:79193689-79193708 None:intergenic 30.0%
GGAAGAAATAAAGTTATGAC+TGG - chr1.2:79193017-79193036 MS.gene86920:CDS 30.0%
GTGTCTTATAAAAAGGGATA+AGG - chr1.2:79192771-79192790 MS.gene86920:CDS 30.0%
GTTATGATTAGTAACTATGC+CGG - chr1.2:79193406-79193425 MS.gene86920:intron 30.0%
GTTTAATAGAAAGCTATACC+TGG + chr1.2:79193844-79193863 None:intergenic 30.0%
TAATGTTACCACATACCTTA+TGG - chr1.2:79194246-79194265 MS.gene86920:intron 30.0%
TAGTGATGGTAAAAGAAAGT+TGG - chr1.2:79194933-79194952 MS.gene86920:CDS 30.0%
TATCAATTGCTTGATCATCA+AGG + chr1.2:79194678-79194697 None:intergenic 30.0%
TATGCTGTAAATTATGTTCG+TGG - chr1.2:79191939-79191958 MS.gene86920:CDS 30.0%
TCCAAATATTACTTGTGCTA+TGG + chr1.2:79193812-79193831 None:intergenic 30.0%
TCTACATGGTGATATATGAT+TGG + chr1.2:79192222-79192241 None:intergenic 30.0%
TGAAACGGTGTCTTATAAAA+AGG - chr1.2:79192764-79192783 MS.gene86920:CDS 30.0%
TGGCAGCATATTAAATTTCA+TGG + chr1.2:79192117-79192136 None:intergenic 30.0%
TGGTTCGAAAAACTACAATT+CGG - chr1.2:79194152-79194171 MS.gene86920:CDS 30.0%
TGTCATGTGCTCATATAATA+GGG - chr1.2:79192337-79192356 MS.gene86920:intron 30.0%
! ACTAAAAGTGTAAGCAACAT+AGG - chr1.2:79193742-79193761 MS.gene86920:intron 30.0%
! AGAACATACAGGTTTTACAA+CGG - chr1.2:79194526-79194545 MS.gene86920:CDS 30.0%
! ATTTGAAAGAGCACAAGTTA+AGG - chr1.2:79194849-79194868 MS.gene86920:CDS 30.0%
! TCCAATTGCTTATGCATTTT+TGG + chr1.2:79192137-79192156 None:intergenic 30.0%
! TGTGTTTTTCTGGATCATTA+TGG + chr1.2:79194626-79194645 None:intergenic 30.0%
! TTAAGTTAGCTTGACATGTA+AGG + chr1.2:79192473-79192492 None:intergenic 30.0%
! TTATATTTGAGACTTGAGAC+CGG - chr1.2:79194347-79194366 MS.gene86920:intron 30.0%
! TTGAAATGGATGTGTTTTTC+TGG + chr1.2:79194636-79194655 None:intergenic 30.0%
!! CTAAGTAATGTTGATAGTGA+TGG - chr1.2:79194919-79194938 MS.gene86920:CDS 30.0%
!! CTGCTTATGATGTTGATAAT+TGG - chr1.2:79192036-79192055 MS.gene86920:intron 30.0%
!! GCATGTTTTTGACTAGTAAT+TGG + chr1.2:79191462-79191481 None:intergenic 30.0%
!! GGTTTTATGTAAAGGTAGTT+CGG - chr1.2:79194173-79194192 MS.gene86920:CDS 30.0%
!! TTACAAATGGAGAAGCATTA+AGG - chr1.2:79195022-79195041 MS.gene86920:CDS 30.0%
AAAACCTGTATGTTCTACCA+GGG + chr1.2:79194522-79194541 None:intergenic 35.0%
AAAGTGTAAGCAACATAGGA+GGG - chr1.2:79193746-79193765 MS.gene86920:intron 35.0%
AAGCAAGTGCTAAAGATGAT+AGG - chr1.2:79191982-79192001 MS.gene86920:CDS 35.0%
AATAGCAGTAGAAATTTCCC+TGG - chr1.2:79194502-79194521 MS.gene86920:CDS 35.0%
AATGTTTGCACTGCATTCTT+TGG - chr1.2:79194376-79194395 MS.gene86920:CDS 35.0%
ACCAATCTATAGAAGCAGAA+GGG - chr1.2:79193596-79193615 MS.gene86920:intron 35.0%
ACTTCTGTAGTCCTTGTAAA+GGG + chr1.2:79193453-79193472 None:intergenic 35.0%
AGCTATGTACAATGTGGATT+TGG - chr1.2:79191815-79191834 MS.gene86920:CDS 35.0%
AGGGAAATTTCTACTGCTAT+TGG + chr1.2:79194503-79194522 None:intergenic 35.0%
ATATTATGGGTAGGTTATCC+TGG - chr1.2:79193769-79193788 MS.gene86920:intron 35.0%
ATATTTGGAGACTATAACCC+AGG - chr1.2:79193823-79193842 MS.gene86920:intron 35.0%
CAAAAACATGCATGTGACAA+AGG - chr1.2:79191471-79191490 MS.gene86920:CDS 35.0%
CAAATTGAACCAAAGCAAAC+CGG + chr1.2:79193997-79194016 None:intergenic 35.0%
CAACATAGGAGGGATATTAT+GGG - chr1.2:79193756-79193775 MS.gene86920:intron 35.0%
CATAGCTATCATAGGTACAA+AGG + chr1.2:79192514-79192533 None:intergenic 35.0%
CGAACTTAACTAAACCAATC+CGG - chr1.2:79193975-79193994 MS.gene86920:intron 35.0%
CGCGAAAAACATTCACATTA+GGG + chr1.2:79191860-79191879 None:intergenic 35.0%
CTTGTAAAGGGCTTGAATAT+CGG + chr1.2:79193441-79193460 None:intergenic 35.0%
GACTATGTAGTTGTATACCA+AGG + chr1.2:79193173-79193192 None:intergenic 35.0%
GATTGTGGATACCATGTAAA+TGG + chr1.2:79194439-79194458 None:intergenic 35.0%
GCATATCTAGAAACAACCAA+AGG + chr1.2:79191924-79191943 None:intergenic 35.0%
GCCAAAAATGCATAAGCAAT+TGG - chr1.2:79192133-79192152 MS.gene86920:intron 35.0%
GCCATAGCACAAGTAATATT+TGG - chr1.2:79193808-79193827 MS.gene86920:intron 35.0%
GGGAAATCTCTCTATGAATT+TGG - chr1.2:79194547-79194566 MS.gene86920:CDS 35.0%
GTGTCATGTGCTCATATAAT+AGG - chr1.2:79192336-79192355 MS.gene86920:intron 35.0%
GTTACTAATCATAACTGTGG+TGG + chr1.2:79193401-79193420 None:intergenic 35.0%
GTTCCACTGAAACAATTGAT+AGG - chr1.2:79194826-79194845 MS.gene86920:CDS 35.0%
GTTCTGCTTGAAAACAACAA+AGG - chr1.2:79193313-79193332 MS.gene86920:CDS 35.0%
TAAAACCTGTATGTTCTACC+AGG + chr1.2:79194523-79194542 None:intergenic 35.0%
TACATAGTCCTAATGAGACT+TGG - chr1.2:79193184-79193203 MS.gene86920:CDS 35.0%
TACTTCTGTAGTCCTTGTAA+AGG + chr1.2:79193454-79193473 None:intergenic 35.0%
TATGGTCTCATATATCATGC+AGG - chr1.2:79193058-79193077 MS.gene86920:CDS 35.0%
TCGCGAAAAACATTCACATT+AGG + chr1.2:79191861-79191880 None:intergenic 35.0%
TCTCTCTATGAATTTGGCTA+TGG - chr1.2:79194553-79194572 MS.gene86920:CDS 35.0%
TCTTTAGTGCATACATCAGA+TGG + chr1.2:79193253-79193272 None:intergenic 35.0%
TGCTAAAGATGATAGGCTTA+AGG - chr1.2:79191989-79192008 MS.gene86920:CDS 35.0%
TGCTCATATAATAGGGTGAA+TGG - chr1.2:79192344-79192363 MS.gene86920:intron 35.0%
TTGCAAATGGAAGTGACTTT+GGG + chr1.2:79193222-79193241 None:intergenic 35.0%
TTGTGGATACCATGTAAATG+GGG + chr1.2:79194437-79194456 None:intergenic 35.0%
TTTGCAAATGGAAGTGACTT+TGG + chr1.2:79193223-79193242 None:intergenic 35.0%
! AAAAGTGTAAGCAACATAGG+AGG - chr1.2:79193745-79193764 MS.gene86920:intron 35.0%
! AAAGAGCACAAGTTAAGGTT+GGG - chr1.2:79194854-79194873 MS.gene86920:CDS 35.0%
! AAGAACAATGGACCTTTTGA+CGG - chr1.2:79194748-79194767 MS.gene86920:CDS 35.0%
! ATATTTACGGAGATTTTCGC+AGG - chr1.2:79192241-79192260 MS.gene86920:intron 35.0%
! ATTTGAGACCATAAGGTATG+TGG + chr1.2:79194257-79194276 None:intergenic 35.0%
! GAACATACAGGTTTTACAAC+GGG - chr1.2:79194527-79194546 MS.gene86920:CDS 35.0%
! GTTTTTCTGGATCATTATGG+TGG + chr1.2:79194623-79194642 None:intergenic 35.0%
! TTGACATTTTCGCTTGTGAT+TGG - chr1.2:79194721-79194740 MS.gene86920:CDS 35.0%
!! ATAATTGGAAAGGCGTTGAT+CGG - chr1.2:79192051-79192070 MS.gene86920:intron 35.0%
!! ATTTTAGTCATAATGGCAGC+AGG - chr1.2:79193700-79193719 MS.gene86920:intron 35.0%
!! CTCTAACATTCGATTTGGTT+TGG - chr1.2:79194051-79194070 MS.gene86920:CDS 35.0%
!! GATAGTGAAGTGTTGCAAAA+TGG + chr1.2:79191519-79191538 None:intergenic 35.0%
!! GGTAAAAGAAAGTTGGTGAT+TGG - chr1.2:79194940-79194959 MS.gene86920:CDS 35.0%
!! TCATGTGGTGTTAGTGTTTT+TGG - chr1.2:79194774-79194793 MS.gene86920:CDS 35.0%
!!! AATGGAAAGTTGCTGGTTTT+AGG + chr1.2:79191501-79191520 None:intergenic 35.0%
!!! ATTTGGCTGGTTTGACATTT+TGG - chr1.2:79191832-79191851 MS.gene86920:CDS 35.0%
AACACACAGCTCTTAAATGG+AGG + chr1.2:79192299-79192318 None:intergenic 40.0%
ACAAGCTTCTCTTGAAATCC+AGG + chr1.2:79193646-79193665 None:intergenic 40.0%
AGAGAAGCTTGTGAAAGATG+TGG - chr1.2:79193654-79193673 MS.gene86920:intron 40.0%
AGCTAGAGCTATGTACAATG+TGG - chr1.2:79191809-79191828 MS.gene86920:CDS 40.0%
ATAGGAGGGATATTATGGGT+AGG - chr1.2:79193760-79193779 MS.gene86920:intron 40.0%
ATCTATTGTAGACCAAGCCT+TGG - chr1.2:79193153-79193172 MS.gene86920:CDS 40.0%
ATCTTATGAAACCAGAGCCA+AGG - chr1.2:79191536-79191555 MS.gene86920:CDS 40.0%
ATGTACAATGTGGATTTGGC+TGG - chr1.2:79191819-79191838 MS.gene86920:CDS 40.0%
ATTTAAGAGCTGTGTGTTGG+AGG - chr1.2:79192301-79192320 MS.gene86920:intron 40.0%
ATTTCCCTGGTAGAACATAC+AGG - chr1.2:79194515-79194534 MS.gene86920:CDS 40.0%
CAAAGAAGCCAAGTCTCATT+AGG + chr1.2:79193195-79193214 None:intergenic 40.0%
CAACTAGTAAACCCTTCTAC+CGG - chr1.2:79191597-79191616 MS.gene86920:CDS 40.0%
CGATATTCAAGCCCTTTACA+AGG - chr1.2:79193439-79193458 MS.gene86920:intron 40.0%
CTCATGTTCATGTCTGTCAT+TGG + chr1.2:79194478-79194497 None:intergenic 40.0%
CTTCATGGTGTCTCAAATGT+AGG - chr1.2:79191666-79191685 MS.gene86920:CDS 40.0%
GACCAATCTATAGAAGCAGA+AGG - chr1.2:79193595-79193614 MS.gene86920:intron 40.0%
GCAACATAGGAGGGATATTA+TGG - chr1.2:79193755-79193774 MS.gene86920:intron 40.0%
GCAGGTTTAAACATGAACTG+CGG - chr1.2:79193076-79193095 MS.gene86920:CDS 40.0%
GTGATTGGTGTGAAGAACAA+TGG - chr1.2:79194736-79194755 MS.gene86920:CDS 40.0%
GTTGCAAAATGGAAAGTTGC+TGG + chr1.2:79191508-79191527 None:intergenic 40.0%
TCACTTCCATTTGCAAACCT+TGG - chr1.2:79193226-79193245 MS.gene86920:CDS 40.0%
TCCAACACACAGCTCTTAAA+TGG + chr1.2:79192302-79192321 None:intergenic 40.0%
TCCATTTAAGAGCTGTGTGT+TGG - chr1.2:79192298-79192317 MS.gene86920:intron 40.0%
TGTAGTTGTATACCAAGGCT+TGG + chr1.2:79193168-79193187 None:intergenic 40.0%
TGTTTCAGACTGTGATTCAG+TGG - chr1.2:79192878-79192897 MS.gene86920:CDS 40.0%
TTTAAGAGCTGTGTGTTGGA+GGG - chr1.2:79192302-79192321 MS.gene86920:intron 40.0%
! AGAGTTAACTTGACATTGCC+TGG - chr1.2:79193625-79193644 MS.gene86920:intron 40.0%
! GAAAGAGCACAAGTTAAGGT+TGG - chr1.2:79194853-79194872 MS.gene86920:CDS 40.0%
! TAAACCAATCCGGTTTGCTT+TGG - chr1.2:79193985-79194004 MS.gene86920:intron 40.0%
! TCTTTTTGCAGCAGCAGTTA+AGG - chr1.2:79193531-79193550 MS.gene86920:CDS 40.0%
! TGAATGTTTTTCGCGATCCA+AGG - chr1.2:79191865-79191884 MS.gene86920:CDS 40.0%
! TTCTGGATCATTATGGTGGA+AGG + chr1.2:79194619-79194638 None:intergenic 40.0%
! TTGGTGTTCCTGCTTATGAA+TGG - chr1.2:79191631-79191650 MS.gene86920:CDS 40.0%
!! TCGGTTCCATTTTGATGCTA+AGG - chr1.2:79192070-79192089 MS.gene86920:intron 40.0%
AAAGACTCCTGAAGATGCAG+TGG - chr1.2:79192929-79192948 MS.gene86920:CDS 45.0%
AAAGGGCTTGAATATCGGCA+TGG + chr1.2:79193436-79193455 None:intergenic 45.0%
AACACCAAGCCTTGAAATGC+CGG + chr1.2:79191619-79191638 None:intergenic 45.0%
ACCACATGAGATCCGTCAAA+AGG + chr1.2:79194763-79194782 None:intergenic 45.0%
ACTGTGGTGGCATTTGCATT+TGG + chr1.2:79193388-79193407 None:intergenic 45.0%
ATCTATAGAAGCAGAAGGGC+TGG - chr1.2:79193600-79193619 MS.gene86920:intron 45.0%
ATTACACCTTGGAGAAGGTC+TGG + chr1.2:79192389-79192408 None:intergenic 45.0%
CAGTGCAAACATTACTCCTC+CGG + chr1.2:79194369-79194388 None:intergenic 45.0%
CTCTGATTACACCTTGGAGA+AGG + chr1.2:79192394-79192413 None:intergenic 45.0%
CTTCTCCAAGGTGTAATCAG+AGG - chr1.2:79192392-79192411 MS.gene86920:intron 45.0%
GATGGTCCAAGGTTTGCAAA+TGG + chr1.2:79193235-79193254 None:intergenic 45.0%
GATTGGACAGCACACTATTC+TGG - chr1.2:79194957-79194976 MS.gene86920:CDS 45.0%
GCCCTTCTGCTTCTATAGAT+TGG + chr1.2:79193600-79193619 None:intergenic 45.0%
GTAGGTTATCCTGGTCAAGA+TGG - chr1.2:79193778-79193797 MS.gene86920:intron 45.0%
GTGTCTCAAATGTAGGACCA+GGG - chr1.2:79191673-79191692 MS.gene86920:CDS 45.0%
GTGTTCCTGCTTATGAATGG+TGG - chr1.2:79191634-79191653 MS.gene86920:CDS 45.0%
TAAGCGTGACACTAAGTCCT+TGG + chr1.2:79191556-79191575 None:intergenic 45.0%
TCTGACCACCATTCATAAGC+AGG + chr1.2:79191642-79191661 None:intergenic 45.0%
TCTTCAGTAACTTACGCGCA+TGG - chr1.2:79193478-79193497 MS.gene86920:intron 45.0%
TGAACCAAAGCAAACCGGAT+TGG + chr1.2:79193992-79194011 None:intergenic 45.0%
TGAATATCGGCATGGTATGC+CGG + chr1.2:79193428-79193447 None:intergenic 45.0%
TGCATACATCAGATGGTCCA+AGG + chr1.2:79193246-79193265 None:intergenic 45.0%
TGCTTATGAATGGTGGTCAG+AGG - chr1.2:79191641-79191660 MS.gene86920:CDS 45.0%
! AACGAACCTTCATACCGGTT+CGG - chr1.2:79194212-79194231 MS.gene86920:intron 45.0%
! ATGTTTTTCGCGATCCAAGG+TGG - chr1.2:79191868-79191887 MS.gene86920:CDS 45.0%
! GTTCGTGGACTACAAGAAGT+TGG - chr1.2:79191954-79191973 MS.gene86920:CDS 45.0%
! TATTTGAGACTTGAGACCGG+AGG - chr1.2:79194350-79194369 MS.gene86920:intron 45.0%
! TGTTTTTCGCGATCCAAGGT+GGG - chr1.2:79191869-79191888 MS.gene86920:CDS 45.0%
! TTTTGCAGCAGCAGTTAAGG+CGG - chr1.2:79193534-79193553 MS.gene86920:CDS 45.0%
!! ACCTTTTGACGGATCTCATG+TGG - chr1.2:79194759-79194778 MS.gene86920:CDS 45.0%
!! AGTGGTGTTGGTTGTTGGAT+TGG - chr1.2:79193573-79193592 MS.gene86920:intron 45.0%
AACTCCAGGGGAAGATCCTT+TGG - chr1.2:79191905-79191924 MS.gene86920:CDS 50.0%
AAGGTGTAATCAGAGGCCAG+TGG - chr1.2:79192399-79192418 MS.gene86920:intron 50.0%
ACAACCAAAGGATCTTCCCC+TGG + chr1.2:79191912-79191931 None:intergenic 50.0%
ACCCTTCTACCGGCATTTCA+AGG - chr1.2:79191607-79191626 MS.gene86920:CDS 50.0%
AGCCTTGAAATGCCGGTAGA+AGG + chr1.2:79191612-79191631 None:intergenic 50.0%
AGGTCTGGATCAGCACATGT+AGG + chr1.2:79192374-79192393 None:intergenic 50.0%
AGGTGTAATCAGAGGCCAGT+GGG - chr1.2:79192400-79192419 MS.gene86920:intron 50.0%
CGGTATGAAGGTTCGTTCAC+CGG + chr1.2:79194209-79194228 None:intergenic 50.0%
CGGTGAACGAACCTTCATAC+CGG - chr1.2:79194207-79194226 MS.gene86920:intron 50.0%
GCAGCTGATAGTATCACTGC+TGG + chr1.2:79191738-79191757 None:intergenic 50.0%
GCAGGTGACAAAGCAAGACT+TGG - chr1.2:79192259-79192278 MS.gene86920:intron 50.0%
GCATTTCCTCCATCTTGACC+AGG + chr1.2:79193790-79193809 None:intergenic 50.0%
GCCTTGAAATGCCGGTAGAA+GGG + chr1.2:79191611-79191630 None:intergenic 50.0%
GGAAGGAGCAGATGCTATGT+GGG + chr1.2:79194602-79194621 None:intergenic 50.0%
GGACAGCACACTATTCTGGT+TGG - chr1.2:79194961-79194980 MS.gene86920:CDS 50.0%
GGTGACAAAGCAAGACTTGG+AGG - chr1.2:79192262-79192281 MS.gene86920:intron 50.0%
GGTGTCTCAAATGTAGGACC+AGG - chr1.2:79191672-79191691 MS.gene86920:CDS 50.0%
GGTTATCCTGGTCAAGATGG+AGG - chr1.2:79193781-79193800 MS.gene86920:intron 50.0%
GTAGTTCGGAACCACAGAAC+CGG - chr1.2:79194187-79194206 MS.gene86920:intron 50.0%
GTGGTAGGTCCCCATTTACA+TGG - chr1.2:79194425-79194444 MS.gene86920:CDS 50.0%
TCTACCGGCATTTCAAGGCT+TGG - chr1.2:79191612-79191631 MS.gene86920:CDS 50.0%
TGACACTAAGTCCTTGGCTC+TGG + chr1.2:79191550-79191569 None:intergenic 50.0%
TGCTATGTGGGTGGAGAATG+TGG + chr1.2:79194590-79194609 None:intergenic 50.0%
TGGAAGGAGCAGATGCTATG+TGG + chr1.2:79194603-79194622 None:intergenic 50.0%
TTATTACCGTCGTCCCGAAC+CGG + chr1.2:79194229-79194248 None:intergenic 50.0%
! ACTGGCCTCTGATTACACCT+TGG + chr1.2:79192400-79192419 None:intergenic 50.0%
! GTTTTTCGCGATCCAAGGTG+GGG - chr1.2:79191870-79191889 MS.gene86920:CDS 50.0%
! TTTGGAGCCACCAAGTTCAG+AGG - chr1.2:79194792-79194811 MS.gene86920:CDS 50.0%
! TTTTTCGCGATCCAAGGTGG+GGG - chr1.2:79191871-79191890 MS.gene86920:CDS 50.0%
!! ACGAACCTTCATACCGGTTC+GGG - chr1.2:79194213-79194232 MS.gene86920:intron 50.0%
!! GATGCAGTGGTGTTGGTTGT+TGG - chr1.2:79193568-79193587 MS.gene86920:CDS 50.0%
AGGAGCAGATGCTATGTGGG+TGG + chr1.2:79194599-79194618 None:intergenic 55.0%
AGGGACGCGTTTCGATAGCA+GGG - chr1.2:79191692-79191711 MS.gene86920:CDS 55.0%
CAAACCCATCTAGACCCCAC+TGG + chr1.2:79192418-79192437 None:intergenic 55.0%
CAACACCACTGCATCAGCAG+AGG + chr1.2:79193563-79193582 None:intergenic 55.0%
CCAAGTTCAGAGGTGGTGAG+TGG - chr1.2:79194802-79194821 MS.gene86920:CDS 55.0%
CCACTCACCACCTCTGAACT+TGG + chr1.2:79194805-79194824 None:intergenic 55.0%
CTCACCACCTCTGAACTTGG+TGG + chr1.2:79194802-79194821 None:intergenic 55.0%
CTCTGCTGATGCAGTGGTGT+TGG - chr1.2:79193561-79193580 MS.gene86920:CDS 55.0%
GATGTGGCTGCTGCTACCAA+AGG - chr1.2:79193670-79193689 MS.gene86920:intron 55.0%
GCAAGAGCCACTGCATCTTC+AGG + chr1.2:79192939-79192958 None:intergenic 55.0%
GCGTGGACAAGAAACTCCAG+GGG - chr1.2:79191893-79191912 MS.gene86920:CDS 55.0%
GCTGATCCAGACCTTCTCCA+AGG - chr1.2:79192380-79192399 MS.gene86920:intron 55.0%
GCTGGAAAGCTAGTAGCACC+AGG + chr1.2:79191720-79191739 None:intergenic 55.0%
GGCGTGGACAAGAAACTCCA+GGG - chr1.2:79191892-79191911 MS.gene86920:CDS 55.0%
GGTGTAATCAGAGGCCAGTG+GGG - chr1.2:79192401-79192420 MS.gene86920:intron 55.0%
GTGGCTCTTGCACTGAAAGC+AGG - chr1.2:79192948-79192967 MS.gene86920:CDS 55.0%
! CGTTTCGATAGCAGGGTACC+TGG - chr1.2:79191699-79191718 MS.gene86920:CDS 55.0%
!! AGGTTCGTTCACCGGTTCTG+TGG + chr1.2:79194201-79194220 None:intergenic 55.0%
!! GCTCTAGAAGCTGCGAAGCA+AGG - chr1.2:79193286-79193305 MS.gene86920:CDS 55.0%
!! TGGTGGTCAGAGGCACTTCA+TGG - chr1.2:79191651-79191670 MS.gene86920:CDS 55.0%
!! TTCATACCGGTTCGGGACGA+CGG - chr1.2:79194220-79194239 MS.gene86920:intron 55.0%
AGAGGCCAGTGGGGTCTAGA+TGG - chr1.2:79192410-79192429 MS.gene86920:intron 60.0%
CAGGGACGCGTTTCGATAGC+AGG - chr1.2:79191691-79191710 MS.gene86920:CDS 60.0%
CTGCTATCGAAACGCGTCCC+TGG + chr1.2:79191693-79191712 None:intergenic 60.0%
GAGGCCAGTGGGGTCTAGAT+GGG - chr1.2:79192411-79192430 MS.gene86920:intron 60.0%
GGAGCCACCAAGTTCAGAGG+TGG - chr1.2:79194795-79194814 MS.gene86920:CDS 60.0%
GGGCGTGGACAAGAAACTCC+AGG - chr1.2:79191891-79191910 MS.gene86920:CDS 60.0%
GTCGTCCCGAACCGGTATGA+AGG + chr1.2:79194221-79194240 None:intergenic 60.0%
GGCAGCCTCTGCTGATGCAG+TGG - chr1.2:79193555-79193574 MS.gene86920:CDS 65.0%
TCTTGTCCACGCCCCCACCT+TGG + chr1.2:79191885-79191904 None:intergenic 65.0%
CGCGATCCAAGGTGGGGGCG+TGG - chr1.2:79191876-79191895 MS.gene86920:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 79191408 79195053 79191408 ID=MS.gene86920
chr1.2 mRNA 79191408 79195053 79191408 ID=MS.gene86920.t1;Parent=MS.gene86920
chr1.2 exon 79194370 79195053 79194370 ID=MS.gene86920.t1.exon1;Parent=MS.gene86920.t1
chr1.2 CDS 79194370 79195053 79194370 ID=cds.MS.gene86920.t1;Parent=MS.gene86920.t1
chr1.2 exon 79194029 79194198 79194029 ID=MS.gene86920.t1.exon2;Parent=MS.gene86920.t1
chr1.2 CDS 79194029 79194198 79194029 ID=cds.MS.gene86920.t1;Parent=MS.gene86920.t1
chr1.2 exon 79193492 79193589 79193492 ID=MS.gene86920.t1.exon3;Parent=MS.gene86920.t1
chr1.2 CDS 79193492 79193589 79193492 ID=cds.MS.gene86920.t1;Parent=MS.gene86920.t1
chr1.2 exon 79192617 79193381 79192617 ID=MS.gene86920.t1.exon4;Parent=MS.gene86920.t1
chr1.2 CDS 79192617 79193381 79192617 ID=cds.MS.gene86920.t1;Parent=MS.gene86920.t1
chr1.2 exon 79191408 79192036 79191408 ID=MS.gene86920.t1.exon5;Parent=MS.gene86920.t1
chr1.2 CDS 79191408 79192036 79191408 ID=cds.MS.gene86920.t1;Parent=MS.gene86920.t1
Gene Sequence

>MS.gene86920

ATGAAAAATCATCTTTTGTTCACGTGTATTTTCTTTTCATTTTTGATACATCCAATTACTAGTCAAAAACATGCATGTGACAAAGGCTCTCCTAAAACCAGCAACTTTCCATTTTGCAACACTTCACTATCTTATGAAACCAGAGCCAAGGACTTAGTGTCACGCTTAACACTTCAAGAAAAAGCACAACAACTAGTAAACCCTTCTACCGGCATTTCAAGGCTTGGTGTTCCTGCTTATGAATGGTGGTCAGAGGCACTTCATGGTGTCTCAAATGTAGGACCAGGGACGCGTTTCGATAGCAGGGTACCTGGTGCTACTAGCTTTCCAGCAGTGATACTATCAGCTGCTAGTTTTAATGAAACATTATGGTACAAAATGGGACAAGTTGTGTCAAATGAAGCTAGAGCTATGTACAATGTGGATTTGGCTGGTTTGACATTTTGGAGCCCTAATGTGAATGTTTTTCGCGATCCAAGGTGGGGGCGTGGACAAGAAACTCCAGGGGAAGATCCTTTGGTTGTTTCTAGATATGCTGTAAATTATGTTCGTGGACTACAAGAAGTTGGTGATGAAGCAAGTGCTAAAGATGATAGGCTTAAGGTTTCTAGCTGTTGTAAGCATTATACTGCTTATGATGTTGATAATTGGAAAGGCGTTGATCGGTTCCATTTTGATGCTAAGGTAATAAAGATGACAAATTAGACCATGAAATTTAATATGCTGCCAAAAATGCATAAGCAATTGGATCATGATGACAGAATAATTTAATGACTATCACCGAACGTGTTAAATATTTTTACAACTGCATCCAATCATATATCACCATGTAGATATTTACGGAGATTTTCGCAGGTGACAAAGCAAGACTTGGAGGATACTTATCAGCCTCCATTTAAGAGCTGTGTGTTGGAGGGACATGTTAGCAGTGTCATGTGCTCATATAATAGGGTGAATGGAATTCCTACATGTGCTGATCCAGACCTTCTCCAAGGTGTAATCAGAGGCCAGTGGGGTCTAGATGGGTTTGTCTCTATCCCTAAGCAATCATTTGATTTTTTTCCTTACATGTCAAGCTAACTTAAACACTAAAGCAATTTATACCTTTGTACCTATGATAGCTATGATAAACTACATACTATCTTAGTCCCTAAATATAGGATTTAGTTGACTAAATTACTAGAATTTGGAAAGTTGGTATAGCAGTATGAAATTTGTTAAGAATTAGTAGTATTGTTTTACAGGAAAGACAACTAATTTATCTTTCTATCATAGTATTTATTACAGGTTGTAGAAAAGGATGTAACGTAATTAAGAGTATGTTGATAAAAATAATAATTAACGCAGTTGAAAATTTGAAACGGTGTCTTATAAAAAGGGATAAGGAAAAATCTCAACATAATCTTATATTTAGAGACAGATAACTAAAAGAAGCTCCCTGATATCTTATACTTTTTTGAACAGATATATTGTTTCAGACTGTGATTCAGTGGAAGTTTATTACGACTCAATTCATTACACAAAGACTCCTGAAGATGCAGTGGCTCTTGCACTGAAAGCAGGTAATCAAGTAGAACTGAAGTTGAAACTGCAAAAAAAATTCTGATTGGGAAGAAATAAAGTTATGACTGGTATTGGATTATTAAATGGTATGGTCTCATATATCATGCAGGTTTAAACATGAACTGCGGCGACTTTCTTAAAAAGTACACTGCAAATGCTGTAAACTTGAAGAAAGTAGATGTATCTATTGTAGACCAAGCCTTGGTATACAACTACATAGTCCTAATGAGACTTGGCTTCTTTGAAAACCCAAAGTCACTTCCATTTGCAAACCTTGGACCATCTGATGTATGCACTAAAGAAAATCAGCAATTGGCTCTAGAAGCTGCGAAGCAAGGAATAGTTCTGCTTGAAAACAACAAAGGAGCTCTTCCTTTATCAAAGACGAAGATAAAAAATTTGGCTGTGATTGGACCAAATGCAAATGCCACCACAGTTATGATTAGTAACTATGCCGGCATACCATGCCGATATTCAAGCCCTTTACAAGGACTACAGAAGTACATTTCTTCAGTAACTTACGCGCATGGTTGCAGTGATGTTAAATGCAGTAATCAGAATCTTTTTGCAGCAGCAGTTAAGGCGGCAGCCTCTGCTGATGCAGTGGTGTTGGTTGTTGGATTGGACCAATCTATAGAAGCAGAAGGGCTGGATAGAGTTAACTTGACATTGCCTGGATTTCAAGAGAAGCTTGTGAAAGATGTGGCTGCTGCTACCAAAGGAACATTGATTTTAGTCATAATGGCAGCAGGCCCAATTGATATTTCTTTCACTAAAAGTGTAAGCAACATAGGAGGGATATTATGGGTAGGTTATCCTGGTCAAGATGGAGGAAATGCCATAGCACAAGTAATATTTGGAGACTATAACCCAGGTATAGCTTTCTATTAAACTATTAAAATACCTACATTTATCTTTACATATTTTGTTACCTATGACAGTTAAAAAAACATTAATTGATCTTTTAAACTGAACTGAAAAATATGAAATGTGCTGATAGTTCACGAACTTAACTAAACCAATCCGGTTTGCTTTGGTTCAATTTGTGAACCATTCGTTATCGTTTGATTTTTTAAACAACTCTAACATTCGATTTGGTTTGGTTTTTGATATTTTTTCAATAATCCTAATTATCAATTTATAATAGCAATTCGATGTACTTTGATTCATAAAAATCAGTTTGGTTCGAAAAACTACAATTCGGTTTTATGTAAAGGTAGTTCGGAACCACAGAACCGGTGAACGAACCTTCATACCGGTTCGGGACGACGGTAATAATGTTACCACATACCTTATGGTCTCAAATATAAACAAACAAGAAACAAGTCTACACATTTAATCCAAGTTTATACCAAACACATAAACTTTTATTTTTCTTATATTTGAGACTTGAGACCGGAGGAGTAATGTTTGCACTGCATTCTTTGGACTAACAGTGAAAATTTTATTTATAGGTGGTAGGTCCCCATTTACATGGTATCCACAATCATATGTTGATCAAGTTCCAATGACAGACATGAACATGAGAGCCAATAGCAGTAGAAATTTCCCTGGTAGAACATACAGGTTTTACAACGGGAAATCTCTCTATGAATTTGGCTATGGATTAAGCTACTCCACATTCTCCACCCACATAGCATCTGCTCCTTCCACCATAATGATCCAGAAAAACACATCCATTTCAAAAACACTTAATAATATCTTCCTTGATGATCAAGCAATTGATATTTCCACCATAAGTTGTTTGAATTTGACATTTTCGCTTGTGATTGGTGTGAAGAACAATGGACCTTTTGACGGATCTCATGTGGTGTTAGTGTTTTTGGAGCCACCAAGTTCAGAGGTGGTGAGTGGTGTTCCACTGAAACAATTGATAGGATTTGAAAGAGCACAAGTTAAGGTTGGGAAGACAGAGTTTGTGACAGTAAAAATTGATATATGCAAATTGCTAAGTAATGTTGATAGTGATGGTAAAAGAAAGTTGGTGATTGGACAGCACACTATTCTGGTTGGAGCTTCTAGTGAAAAACAAGTTACACACCACATTGATATTACAAATGGAGAAGCATTAAGGCCTGAGTGA
Protein sequence

>MS.gene86920.t1

MKNHLLFTCIFFSFLIHPITSQKHACDKGSPKTSNFPFCNTSLSYETRAKDLVSRLTLQEKAQQLVNPSTGISRLGVPAYEWWSEALHGVSNVGPGTRFDSRVPGATSFPAVILSAASFNETLWYKMGQVVSNEARAMYNVDLAGLTFWSPNVNVFRDPRWGRGQETPGEDPLVVSRYAVNYVRGLQEVGDEASAKDDRLKVSSCCKHYTAYDVDNWKGVDRFHFDAKVTKQDLEDTYQPPFKSCVLEGHVSSVMCSYNRVNGIPTCADPDLLQGVIRGQWGLDGYIVSDCDSVEVYYDSIHYTKTPEDAVALALKAGLNMNCGDFLKKYTANAVNLKKVDVSIVDQALVYNYIVLMRLGFFENPKSLPFANLGPSDVCTKENQQLALEAAKQGIVLLENNKGALPLSKTKIKNLAVIGPNANATTVMISNYAGIPCRYSSPLQGLQKYISSVTYAHGCSDVKCSNQNLFAAAVKAAASADAVVLVVGLDQSIEAEGLDRVNLTLPGFQEKLVKDVAAATKGTLILVIMAAGPIDISFTKSVSNIGGILWVGYPGQDGGNAIAQVIFGDYNPGGRSPFTWYPQSYVDQVPMTDMNMRANSSRNFPGRTYRFYNGKSLYEFGYGLSYSTFSTHIASAPSTIMIQKNTSISKTLNNIFLDDQAIDISTISCLNLTFSLVIGVKNNGPFDGSHVVLVFLEPPSSEVVSGVPLKQLIGFERAQVKVGKTEFVTVKIDICKLLSNVDSDGKRKLVIGQHTILVGASSEKQVTHHIDITNGEALRPE