AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35037

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35037.t1 MTR_4g094285 98.708 774 10 0 1 774 1 774 0.0 1587
MS.gene35037.t1 MTR_2g008240 85.032 775 115 1 1 774 1 775 0.0 1362
MS.gene35037.t1 MTR_1g087240 57.332 757 311 5 19 772 8 755 0.0 895
MS.gene35037.t1 MTR_7g109390 57.647 765 302 8 17 772 10 761 0.0 894
MS.gene35037.t1 MTR_2g034720 57.778 765 300 9 14 772 7 754 0.0 879
MS.gene35037.t1 MTR_1g106860 55.757 773 302 12 13 764 6 759 0.0 844
MS.gene35037.t1 MTR_5g030860 48.545 756 363 9 42 774 30 782 0.0 748
MS.gene35037.t1 MTR_3g074060 47.247 781 379 15 14 774 9 776 0.0 717
MS.gene35037.t1 MTR_5g062430 46.164 782 391 13 14 774 10 782 0.0 714
MS.gene35037.t1 MTR_5g062320 46.183 786 394 14 10 774 3 780 0.0 712
MS.gene35037.t1 MTR_5g062370 46.164 782 391 13 14 774 10 782 0.0 702
MS.gene35037.t1 MTR_5g062650 45.812 788 398 14 9 774 5 785 0.0 696
MS.gene35037.t1 MTR_5g062370 49.158 594 281 10 14 594 10 595 0.0 572
MS.gene35037.t1 MTR_6g028090 26.875 640 332 27 54 612 28 612 4.48e-40 157
MS.gene35037.t1 MTR_6g028120 27.414 642 332 28 51 612 6 593 1.01e-39 155
MS.gene35037.t1 MTR_3g079750 27.625 619 354 24 54 612 28 612 7.50e-39 153
MS.gene35037.t1 MTR_6g028100 25.786 636 344 24 54 612 28 612 9.64e-39 153
MS.gene35037.t1 MTR_6g028110 25.513 682 380 28 37 641 13 643 2.87e-37 149
MS.gene35037.t1 MTR_6g028110 25.802 655 351 28 38 612 14 613 1.67e-36 146
MS.gene35037.t1 MTR_3g462820 25.041 603 354 21 54 593 22 589 3.33e-35 142
MS.gene35037.t1 MTR_2g030000 27.068 665 364 26 54 648 30 643 1.70e-34 140
MS.gene35037.t1 MTR_7g086030 25.875 657 351 28 54 637 4 597 8.79e-34 138
MS.gene35037.t1 MTR_7g086030 25.875 657 351 28 54 637 29 622 1.48e-33 137
MS.gene35037.t1 MTR_5g069800 26.677 626 354 26 54 612 5 592 2.07e-33 137
MS.gene35037.t1 MTR_5g069740 25.884 622 352 24 54 612 5 580 7.05e-33 135
MS.gene35037.t1 MTR_7g086010 28.533 375 202 17 89 437 118 452 8.80e-24 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35037.t1 AT5G64570 71.483 782 204 4 5 774 10 784 0.0 1190
MS.gene35037.t1 AT5G09730 68.229 768 232 3 12 774 13 773 0.0 1125
MS.gene35037.t1 AT5G64570 73.046 627 162 2 154 774 1 626 0.0 995
MS.gene35037.t1 AT5G64570 73.898 590 141 3 5 588 10 592 0.0 907
MS.gene35037.t1 AT3G19620 56.275 757 317 7 18 764 6 758 0.0 882
MS.gene35037.t1 AT1G02640 54.941 759 335 4 15 772 6 758 0.0 868
MS.gene35037.t1 AT5G49360 57.702 766 302 11 19 772 15 770 0.0 866
MS.gene35037.t1 AT5G10560 47.656 768 361 9 39 772 30 790 0.0 743
MS.gene35037.t1 AT1G78060 47.340 752 370 11 40 774 25 767 0.0 723
MS.gene35037.t1 AT5G09700 66.598 482 155 2 299 774 45 526 0.0 688
MS.gene35037.t1 AT3G47050 26.942 605 363 23 51 603 7 584 2.58e-39 154
MS.gene35037.t1 AT3G47000 26.688 622 357 22 54 612 10 595 1.56e-38 152
MS.gene35037.t1 AT3G47040 24.733 655 374 24 54 643 10 610 1.23e-36 147
MS.gene35037.t1 AT3G47010 25.926 621 371 23 50 612 7 596 2.21e-36 145
MS.gene35037.t1 AT3G47010 25.645 620 373 22 50 612 7 595 9.41e-36 144
MS.gene35037.t1 AT5G04885 26.518 626 359 24 54 616 30 617 2.61e-35 143
MS.gene35037.t1 AT5G04885 26.518 626 359 24 54 616 30 617 2.61e-35 143
MS.gene35037.t1 AT5G04885 26.518 626 359 24 54 616 47 634 3.00e-35 143
MS.gene35037.t1 AT3G47040 24.478 670 382 25 54 643 10 635 2.12e-34 140
MS.gene35037.t1 AT3G62710 25.731 684 366 28 17 612 11 640 1.19e-33 138
MS.gene35037.t1 AT5G20940 26.572 636 363 26 41 612 18 613 3.07e-32 133
MS.gene35037.t1 AT3G47000 29.885 435 235 20 54 437 10 425 2.18e-31 128
MS.gene35037.t1 AT5G20950 26.034 653 377 28 38 625 90 701 1.33e-30 129
MS.gene35037.t1 AT5G20950 26.060 637 367 27 54 625 26 623 1.74e-30 128
MS.gene35037.t1 AT5G20950 26.060 637 367 27 54 625 26 623 1.74e-30 128
MS.gene35037.t1 AT3G47040 26.786 448 232 18 54 437 10 425 3.52e-30 125
MS.gene35037.t1 AT3G47010 26.915 457 252 18 50 449 7 438 1.07e-27 117
MS.gene35037.t1 AT3G47040 23.348 681 384 26 54 643 10 643 1.23e-25 113
MS.gene35037.t1 AT3G47050 28.713 303 161 11 51 310 7 297 2.28e-23 103
MS.gene35037.t1 AT3G47010 26.150 413 266 15 215 612 29 417 6.41e-23 102
MS.gene35037.t1 AT3G47000 26.225 408 273 15 215 612 29 418 1.91e-21 98.6

Find 190 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGACCACCATTCATATTT+TGG 0.140699 4.4:+20660196 None:intergenic
CACCAAACATAAACATATTT+AGG 0.152579 4.4:-20659887 MS.gene35037:CDS
ATTAAATCATTGGCAGTTAT+TGG 0.184597 4.4:-20658392 MS.gene35037:CDS
CGTGGCAAATGGAAACTAAA+TGG 0.204423 4.4:-20659430 MS.gene35037:CDS
CTCAACTTTCGAGCATAAAT+TGG 0.211723 4.4:-20657157 MS.gene35037:CDS
GCTGACCCTGATCTTCTTAA+AGG 0.226486 4.4:-20659460 MS.gene35037:CDS
TGTTCCTACAATCAAGTAAA+TGG 0.245995 4.4:-20659496 MS.gene35037:CDS
GCTGAAGTGCAGACATCTTT+TGG 0.254948 4.4:+20658503 None:intergenic
TCTTGACCTCTTCCCCATCT+TGG 0.278328 4.4:+20659861 None:intergenic
TGCAGCTTCCTCAGGGGTCT+TGG 0.289885 4.4:+20658886 None:intergenic
GCCATAGCTGATGTCATCTT+TGG 0.300033 4.4:-20657835 MS.gene35037:CDS
TGCCAATGATTTAATAGCTT+TGG 0.304831 4.4:+20658400 None:intergenic
CATGCCTCCTCCAGACATTA+TGG 0.305665 4.4:+20657936 None:intergenic
GACTTGGCTGCAGCTTCCTC+AGG 0.311461 4.4:+20658878 None:intergenic
ATTCGCCAATGCTGGATTCT+TGG 0.315695 4.4:+20660345 None:intergenic
GGGGAAACTGTCTTCTCATT+TGG 0.319056 4.4:-20657195 MS.gene35037:CDS
TCAATCACACAGCTCTTAAA+AGG 0.325703 4.4:+20659541 None:intergenic
ATTGTAGACGGCATTGTTAA+TGG 0.328849 4.4:+20658598 None:intergenic
TTAAATCATTGGCAGTTATT+GGG 0.348888 4.4:-20658391 MS.gene35037:CDS
AATCCAGCATTGGCGAATTA+CGG 0.359386 4.4:-20660340 MS.gene35037:CDS
ATCCAGCATTGGCGAATTAC+GGG 0.371963 4.4:-20660339 MS.gene35037:CDS
TTTAAGAGCTGTGTGATTGA+TGG 0.379593 4.4:-20659538 MS.gene35037:CDS
GAGAATAGGCATAGGAAAAC+TGG 0.380578 4.4:+20660108 None:intergenic
GACCCTAGCAAGCAACCTTA+TGG 0.387544 4.4:-20658536 MS.gene35037:CDS
GCTGTCAAGCAAGGGCTTAT+AGG 0.388682 4.4:-20658629 MS.gene35037:CDS
ATGCGTCTAGGTTTCTTTGA+TGG 0.391137 4.4:-20658560 MS.gene35037:CDS
ATTCTGTGGGTTGGCTATCC+TGG 0.392669 4.4:-20657874 MS.gene35037:CDS
ACCAAACATAAACATATTTA+GGG 0.399180 4.4:-20659886 MS.gene35037:CDS
TTTCCATAAGGTTGCTTGCT+AGG 0.400087 4.4:+20658533 None:intergenic
GGTTGGCTGGTTTAACATAT+TGG 0.401638 4.4:-20659911 MS.gene35037:CDS
CCATAGCTGATGTCATCTTT+GGG 0.402935 4.4:-20657834 MS.gene35037:CDS
AGCAGCAACCTTAAGCTTGT+TGG 0.404848 4.4:+20659755 None:intergenic
TGCCAAAGCTATTAAATCAT+TGG 0.406948 4.4:-20658402 MS.gene35037:CDS
TCCCTAAATATGTTTATGTT+TGG 0.410759 4.4:+20659885 None:intergenic
TGGAGATTCCAACAAGCTTA+AGG 0.418052 4.4:-20659763 MS.gene35037:CDS
ACTAACTGTTGCTGCTGTCC+TGG 0.428145 4.4:+20658000 None:intergenic
TGACAGCACCTTCTGTATAT+CGG 0.431143 4.4:+20658645 None:intergenic
ATAACACCTTTAAGAAGATC+AGG 0.432414 4.4:+20659454 None:intergenic
ACCTTCGTAGTTTCCAATCA+TGG 0.434772 4.4:+20658346 None:intergenic
ATTGGTTAAAGCACCACAAT+TGG 0.441371 4.4:-20657139 MS.gene35037:CDS
ATACATTACCACAGCATTGA+AGG 0.443167 4.4:+20659674 None:intergenic
TGCACCTCAGAAACACTTGT+TGG 0.443221 4.4:-20656938 MS.gene35037:CDS
TTGTGGGAACTAAGGCTGTT+AGG 0.443745 4.4:+20658175 None:intergenic
AGGCGAAAACTGCAGATGTT+TGG 0.443791 4.4:+20660377 None:intergenic
GGCCCAATGCTAATGCTACT+AGG 0.444209 4.4:-20658370 MS.gene35037:CDS
AACCCGTAATTCGCCAATGC+TGG 0.444375 4.4:+20660337 None:intergenic
GACATTATGGCAAGAATCAC+AGG 0.445330 4.4:+20657949 None:intergenic
TTCCATAAGGTTGCTTGCTA+GGG 0.446833 4.4:+20658534 None:intergenic
GCATTGGTGCATTGAACATC+AGG 0.448023 4.4:+20658135 None:intergenic
GTTGGCTATCCTGGAGAAGC+TGG 0.450796 4.4:-20657865 MS.gene35037:CDS
CTCATGTTCATATTGGTCAT+TGG 0.453094 4.4:+20657267 None:intergenic
GTTCCCACAAGCTTTGCTGC+TGG 0.455205 4.4:-20658162 MS.gene35037:CDS
CAGCTTCACGAGCTAGCTCC+TGG 0.458313 4.4:+20658477 None:intergenic
AGGATTAACATACTTCTTCC+AGG 0.461097 4.4:-20658018 MS.gene35037:CDS
ATCCGGCCTCATGTTCATAT+TGG 0.462557 4.4:+20657260 None:intergenic
AAAGAAGCTGCAATGAGAAT+AGG 0.464509 4.4:+20660094 None:intergenic
TCCCTTTGTTCTTGATCCTG+AGG 0.465656 4.4:+20657016 None:intergenic
CGGTGCAAATCTTGCAATAG+AGG 0.465750 4.4:-20658055 MS.gene35037:CDS
GGGTAGCCTGTAGCAGGATC+CGG 0.467218 4.4:+20657243 None:intergenic
CTTGCTAGCAAATATGCAGC+AGG 0.468402 4.4:-20659816 MS.gene35037:CDS
TGTAAGTCACTTGATGTTGT+TGG 0.469882 4.4:-20657072 MS.gene35037:CDS
GTGATTCTTGCCATAATGTC+TGG 0.471490 4.4:-20657946 MS.gene35037:CDS
CTTCATGGAGTCTCTAACAT+TGG 0.474694 4.4:-20660169 MS.gene35037:CDS
TGAGGTGCATTGTGCACTGC+AGG 0.474909 4.4:+20656952 None:intergenic
GCTGTTGATGTAAGCAGACT+TGG 0.476218 4.4:-20660223 MS.gene35037:CDS
GAGAAACCTTACCTTTCCTA+TGG 0.476556 4.4:+20660051 None:intergenic
TGGTGGTCTGAAGCACTTCA+TGG 0.476761 4.4:-20660184 MS.gene35037:CDS
AGCAAAGCTTGTGGGAACTA+AGG 0.478113 4.4:+20658167 None:intergenic
ATGTAGTTTACCTGCTAGAA+TGG 0.479155 4.4:+20658856 None:intergenic
ACCTCAGGATCAAGAACAAA+GGG 0.481557 4.4:-20657017 MS.gene35037:CDS
CATATTTGCTAGCAAGTAAA+GGG 0.484061 4.4:+20659823 None:intergenic
CATATTTAGGGATCCAAGAT+GGG 0.485529 4.4:-20659874 MS.gene35037:CDS
ACATATTTAGGGATCCAAGA+TGG 0.488212 4.4:-20659875 MS.gene35037:CDS
TTTGGACCAAGGTTTCCATA+AGG 0.489120 4.4:+20658521 None:intergenic
ATGCACAATGTAGGGTTGGC+TGG 0.489261 4.4:-20659924 MS.gene35037:CDS
GCATATTTGCTAGCAAGTAA+AGG 0.490578 4.4:+20659822 None:intergenic
TTGAGTGTGGTTGATGAACT+TGG 0.490808 4.4:-20656844 MS.gene35037:CDS
AAGGTGTTATCCGTGGCAAA+TGG 0.492172 4.4:-20659441 MS.gene35037:CDS
CTTTCCTATGGTTTGAAACA+AGG 0.492728 4.4:+20660063 None:intergenic
GGCATCGTCTAGTGCAGCAT+TGG 0.492888 4.4:+20658119 None:intergenic
GCAAGAGCAATGCACAATGT+AGG 0.493522 4.4:-20659933 MS.gene35037:CDS
CTACCAGGGTAGCCTGTAGC+AGG 0.493617 4.4:+20657237 None:intergenic
ATGAGGCCGGATCCTGCTAC+AGG 0.493861 4.4:-20657249 MS.gene35037:CDS
CTTTAAATCATCACGCTCAA+AGG 0.498511 4.4:+20656766 None:intergenic
AATGCTACTAGGGCCATGAT+TGG 0.501422 4.4:-20658359 MS.gene35037:CDS
GGTCTACAACAAACTGATGA+TGG 0.502433 4.4:-20659783 MS.gene35037:CDS
TTCGAGAAAGTACACTTGAC+AGG 0.502478 4.4:-20656913 MS.gene35037:CDS
TAGCCTTTGATATTCACCTC+AGG 0.509602 4.4:-20657032 MS.gene35037:CDS
TGTCTGCACTTCAGCAAACC+AGG 0.511708 4.4:-20658495 MS.gene35037:CDS
AGAAGTTGCAAATGTAGCTA+AGG 0.511795 4.4:-20657974 MS.gene35037:CDS
GCTTCCTTGTTTCAAACCAT+AGG 0.512430 4.4:-20660067 MS.gene35037:CDS
TTTACCTGCTAGAATGGACT+TGG 0.512571 4.4:+20658862 None:intergenic
GTGGCAAATGGAAACTAAAT+GGG 0.513107 4.4:-20659429 MS.gene35037:intron
GCTCGTGAAGCTGCAAGGCA+AGG 0.513237 4.4:-20658467 MS.gene35037:CDS
ACTGTCTTCTCATTTGGAGA+TGG 0.513776 4.4:-20657189 MS.gene35037:CDS
GCAGCTCCACCAGCTTCTCC+AGG 0.518655 4.4:+20657856 None:intergenic
CTTGTTTCAAACCATAGGAA+AGG 0.519277 4.4:-20660062 MS.gene35037:intron
ATAGGAAAACTGGTTGCTCC+AGG 0.520085 4.4:+20660118 None:intergenic
ACTTGGCTGCAGCTTCCTCA+GGG 0.522958 4.4:+20658879 None:intergenic
CAAGAGCAATGCACAATGTA+GGG 0.523520 4.4:-20659932 MS.gene35037:CDS
TAAAATCTGTATGTTCTACC+AGG 0.524873 4.4:+20657222 None:intergenic
CAAACGTGATCCTCAGCTAA+AGG 0.526847 4.4:+20657108 None:intergenic
CTCGTGAAGCTGCAAGGCAA+GGG 0.532764 4.4:-20658466 MS.gene35037:CDS
GCCCTAGTAGCATTAGCATT+GGG 0.533958 4.4:+20658368 None:intergenic
TTGGGATTCCAAAATATGAA+TGG 0.535603 4.4:-20660204 MS.gene35037:CDS
GCAAGTAAAGGGTCTTCTCC+TGG 0.538903 4.4:+20659834 None:intergenic
ATCACAAGCATTCTGTGGGT+TGG 0.539372 4.4:-20657883 MS.gene35037:CDS
GGAGTCTCTAACATTGGTCC+AGG 0.541530 4.4:-20660163 MS.gene35037:CDS
TGGATTCTTGGCAACATCGC+AGG 0.541711 4.4:+20660357 None:intergenic
GACAGGAAAATCAGAAGCAC+TGG 0.541961 4.4:-20656896 MS.gene35037:CDS
CAGCCAGCAGCAAAGCTTGT+GGG 0.542585 4.4:+20658159 None:intergenic
CAGAGGAAAACAGAGACGTT+AGG 0.545414 4.4:+20660451 None:intergenic
AGCAATGCACAATGTAGGGT+TGG 0.545688 4.4:-20659928 MS.gene35037:CDS
GGCTATCCTGGAGAAGCTGG+TGG 0.546561 4.4:-20657862 MS.gene35037:CDS
TGTCGCAAAATTATTGTAGA+CGG 0.546588 4.4:+20658586 None:intergenic
GGTGTCTGTTCCTTTAGCTG+AGG 0.552719 4.4:-20657118 MS.gene35037:CDS
AGCTAGCTCGTGAAGCTGCA+AGG 0.553456 4.4:-20658472 MS.gene35037:CDS
GGCCCTAGTAGCATTAGCAT+TGG 0.555454 4.4:+20658367 None:intergenic
ACAGAAGGTGCTGTCAAGCA+AGG 0.561917 4.4:-20658638 MS.gene35037:CDS
TTAATAGCTTTGGCATTCAG+AGG 0.562815 4.4:+20658410 None:intergenic
GCAGCCAAGTCCATTCTAGC+AGG 0.563938 4.4:-20658866 MS.gene35037:intron
AAAAGATCACAAGCATTCTG+TGG 0.568704 4.4:-20657888 MS.gene35037:CDS
GATCCTGAGGTGAATATCAA+AGG 0.569135 4.4:+20657029 None:intergenic
AGGGATCCAAGATGGGGAAG+AGG 0.570000 4.4:-20659867 MS.gene35037:CDS
AAGGTTGACATTGCAAGAGA+AGG 0.572395 4.4:-20660266 MS.gene35037:CDS
AAGCAACCTTATGGAAACCT+TGG 0.575163 4.4:-20658527 MS.gene35037:CDS
GCCCAATGCTAATGCTACTA+GGG 0.577253 4.4:-20658369 MS.gene35037:CDS
TTGACATTGCAAGAGAAGGT+TGG 0.577392 4.4:-20660262 MS.gene35037:CDS
CACAGCTCTTAAAAGGTGGT+TGG 0.578720 4.4:+20659548 None:intergenic
CAGAAGGTGCTGTCAAGCAA+GGG 0.579821 4.4:-20658637 MS.gene35037:CDS
GGAAAACATATGCTTCATGT+AGG 0.580882 4.4:-20656802 MS.gene35037:CDS
TTTGCGACATTAATGCGTCT+AGG 0.581567 4.4:-20658572 MS.gene35037:CDS
CAGCATATGATGTTGATGAC+TGG 0.584387 4.4:-20659716 MS.gene35037:CDS
CACCTCAGGATCAAGAACAA+AGG 0.587124 4.4:-20657018 MS.gene35037:CDS
CATTTAGTTTCCATTTGCCA+CGG 0.587656 4.4:+20659431 None:intergenic
TTTCTTGGCCGATATACAGA+AGG 0.588531 4.4:-20658653 MS.gene35037:CDS
AAAGATCACAAGCATTCTGT+GGG 0.588555 4.4:-20657887 MS.gene35037:CDS
CCCAAAGATGACATCAGCTA+TGG 0.590854 4.4:+20657834 None:intergenic
GACCAATATGAACATGAGGC+CGG 0.590969 4.4:-20657262 MS.gene35037:CDS
AGAAATTGAGTGTGGATGCA+AGG 0.597818 4.4:-20660306 MS.gene35037:CDS
CTGTTGATGTAAGCAGACTT+GGG 0.597835 4.4:-20660222 MS.gene35037:CDS
TGTTGCCAAGAATCCAGCAT+TGG 0.599346 4.4:-20660350 MS.gene35037:CDS
TAGAGGCAGAGAGTCATGAC+AGG 0.601562 4.4:-20658038 MS.gene35037:CDS
GGGTGAAAGACCTTGTGAGA+AGG 0.601719 4.4:-20660285 MS.gene35037:CDS
ATTTGCACCGACTACAATCA+CGG 0.602874 4.4:+20658068 None:intergenic
ACTTTGTCTACATATGATTG+TGG 0.603286 4.4:+20657291 None:intergenic
CATCAACAGCAGAATTCACA+AGG 0.603921 4.4:+20660236 None:intergenic
GGTTGCTCCAGGTATAACAT+TGG 0.604127 4.4:+20660129 None:intergenic
CTTCTTAAAGGTGTTATCCG+TGG 0.607358 4.4:-20659448 MS.gene35037:CDS
ACAGCCAGCAGCAAAGCTTG+TGG 0.607399 4.4:+20658158 None:intergenic
GCGTTACACCTTCAATGCTG+TGG 0.608802 4.4:-20659682 MS.gene35037:intron
CATAATGTCTGGAGGAGGCA+TGG 0.611672 4.4:-20657935 MS.gene35037:CDS
CTTGGCTGCAGCTTCCTCAG+GGG 0.612324 4.4:+20658880 None:intergenic
CTTGCCATAATGTCTGGAGG+AGG 0.615660 4.4:-20657940 MS.gene35037:CDS
GAAGTTGCAAATGTAGCTAA+GGG 0.617135 4.4:-20657973 MS.gene35037:CDS
TATGCAGCAGGGTATGTGAA+AGG 0.622859 4.4:-20659804 MS.gene35037:CDS
AAAGGTGATGTATATTTGCA+TGG 0.623811 4.4:+20658204 None:intergenic
AAAGCCAACAAGTGTTTCTG+AGG 0.625434 4.4:+20656934 None:intergenic
GAAATTGAGTGTGGATGCAA+GGG 0.626342 4.4:-20660305 MS.gene35037:CDS
GATGCAACCGTGATTGTAGT+CGG 0.629741 4.4:-20658075 MS.gene35037:CDS
TGTTTGTAATGATTTGAGTG+TGG 0.631283 4.4:-20656857 MS.gene35037:CDS
GGCAACAGAAAAGTTGCCCT+AGG 0.635515 4.4:-20656823 MS.gene35037:CDS
TGATTGTGGATACCATGTCA+TGG 0.635666 4.4:+20657305 None:intergenic
GCTGCAATGAGAATAGGCAT+AGG 0.636909 4.4:+20660100 None:intergenic
AAATATACATCACCTTTGCA+AGG 0.638543 4.4:-20658198 MS.gene35037:CDS
ATCACACAGCTCTTAAAAGG+TGG 0.639170 4.4:+20659544 None:intergenic
AAAGGAACAGACACCAATTG+TGG 0.639642 4.4:+20657126 None:intergenic
ACAGGATTGGATTTGAACTG+TGG 0.643755 4.4:-20658680 MS.gene35037:intron
GATTGTGGATACCATGTCAT+GGG 0.644060 4.4:+20657306 None:intergenic
AAGGCTGTTAGGCCTTGCAA+AGG 0.647052 4.4:+20658186 None:intergenic
TAACACCTTTAAGAAGATCA+GGG 0.647148 4.4:+20659455 None:intergenic
GGATTCCAAAATATGAATGG+TGG 0.648983 4.4:-20660201 MS.gene35037:CDS
GATCCTGCTACAGGCTACCC+TGG 0.649738 4.4:-20657240 MS.gene35037:CDS
CAATGACCAATATGAACATG+AGG 0.652360 4.4:-20657266 MS.gene35037:CDS
GCAATGTCAACCTTCTCACA+AGG 0.660006 4.4:+20660275 None:intergenic
CAAACAGAACAAAGAAACAG+AGG 0.661188 4.4:+20660434 None:intergenic
ACCACCACTCAGCAAAAGTG+TGG 0.664666 4.4:+20660411 None:intergenic
AGATCAGGGTCAGCACAAGT+CGG 0.668758 4.4:+20659469 None:intergenic
AAAATCTGTATGTTCTACCA+GGG 0.669451 4.4:+20657223 None:intergenic
GGAAGAGGTCAAGAAACACC+AGG 0.670289 4.4:-20659852 MS.gene35037:CDS
TTGCTAGCAAATATGCAGCA+GGG 0.674352 4.4:-20659815 MS.gene35037:CDS
TATGATGTTGATGACTGGAA+AGG 0.681867 4.4:-20659711 MS.gene35037:CDS
ATATTTAGGGATCCAAGATG+GGG 0.682736 4.4:-20659873 MS.gene35037:CDS
GTGGAAGATTACCCATGACA+TGG 0.684813 4.4:-20657317 MS.gene35037:CDS
GGTGCATTGTGCACTGCAGG+AGG 0.684891 4.4:+20656955 None:intergenic
CTGCAACAAGAAATTGAGTG+TGG 0.685493 4.4:-20660314 MS.gene35037:CDS
GCCATGATTGGAAACTACGA+AGG 0.687224 4.4:-20658347 MS.gene35037:intron
AATCACGGTTGCATCTGCTG+AGG 0.690719 4.4:+20658083 None:intergenic
ATTCTTGCCATAATGTCTGG+AGG 0.713271 4.4:-20657943 MS.gene35037:CDS
ACATTACACCAAGACCCCTG+AGG 0.763610 4.4:-20658894 MS.gene35037:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTGTTTATATTTGAA+TGG + chr4.4:20657882-20657901 None:intergenic 10.0%
!!! CATATAAAAGATAATTTTTT+TGG + chr4.4:20658048-20658067 None:intergenic 10.0%
!! CATATATTATTGAGAATTAT+TGG + chr4.4:20659794-20659813 None:intergenic 15.0%
!!! AATTTTTTTGGATGTAAATT+GGG + chr4.4:20658036-20658055 None:intergenic 15.0%
!!! CAACTTAATTTTTTTTATTC+AGG - chr4.4:20659904-20659923 MS.gene35037:CDS 15.0%
!!! TAATTTTTTTGGATGTAAAT+TGG + chr4.4:20658037-20658056 None:intergenic 15.0%
!! AATGATAATTCTGTAATATG+AGG + chr4.4:20659474-20659493 None:intergenic 20.0%
!! ACCAAACATAAACATATTTA+GGG - chr4.4:20657357-20657376 MS.gene35037:intron 20.0%
!!! AAATTTTGATTTTACAGGAT+TGG - chr4.4:20658550-20658569 MS.gene35037:CDS 20.0%
!!! AACAACTTTGATTTTAAACT+CGG - chr4.4:20659567-20659586 MS.gene35037:CDS 20.0%
!!! AACATACAGATTTTATAAAG+GGG - chr4.4:20660029-20660048 MS.gene35037:intron 20.0%
!!! AACTTTCAAATGATGTTTTA+TGG - chr4.4:20657259-20657278 MS.gene35037:CDS 20.0%
!!! AGAACATACAGATTTTATAA+AGG - chr4.4:20660027-20660046 MS.gene35037:intron 20.0%
!!! ATTTTGCCTATAAAAGTTAT+AGG + chr4.4:20658939-20658958 None:intergenic 20.0%
!!! CTTAATTTTTTTTATTCAGG+TGG - chr4.4:20659907-20659926 MS.gene35037:CDS 20.0%
!!! CTTTTTATTAGTTTTAGCAA+AGG + chr4.4:20659340-20659359 None:intergenic 20.0%
!!! GAACATACAGATTTTATAAA+GGG - chr4.4:20660028-20660047 MS.gene35037:intron 20.0%
!!! TAATTGTTGTTGATTTTGTT+AGG - chr4.4:20657644-20657663 MS.gene35037:intron 20.0%
!!! TGTACAAATTTTGATTTTAC+AGG - chr4.4:20658545-20658564 MS.gene35037:CDS 20.0%
! AAATCGAAAACAGAAAAGAA+TGG + chr4.4:20658236-20658255 None:intergenic 25.0%
! AGTGAGATAAAAGAATTAAG+AGG + chr4.4:20657859-20657878 None:intergenic 25.0%
! ATTAAATCATTGGCAGTTAT+TGG - chr4.4:20658851-20658870 MS.gene35037:intron 25.0%
! CACCAAACATAAACATATTT+AGG - chr4.4:20657356-20657375 MS.gene35037:intron 25.0%
! GAATTATCATTCCAATAACT+TGG - chr4.4:20659483-20659502 MS.gene35037:CDS 25.0%
! TCACTAGGATCTAATTATAT+GGG - chr4.4:20659870-20659889 MS.gene35037:CDS 25.0%
! TCCCTAAATATGTTTATGTT+TGG + chr4.4:20657361-20657380 None:intergenic 25.0%
! TTAAATCATTGGCAGTTATT+GGG - chr4.4:20658852-20658871 MS.gene35037:intron 25.0%
! TTACACAATCACATATTCAT+AGG - chr4.4:20658419-20658438 MS.gene35037:CDS 25.0%
!! TTTTCCATTTACTTGATTGT+AGG + chr4.4:20657754-20657773 None:intergenic 25.0%
!!! AGCTTCTTTAGAATTATACA+AGG - chr4.4:20658499-20658518 MS.gene35037:CDS 25.0%
AAAATCTGTATGTTCTACCA+GGG + chr4.4:20660023-20660042 None:intergenic 30.0%
AAAGGTGATGTATATTTGCA+TGG + chr4.4:20659042-20659061 None:intergenic 30.0%
AAATATACATCACCTTTGCA+AGG - chr4.4:20659045-20659064 MS.gene35037:intron 30.0%
ACTTTGTCTACATATGATTG+TGG + chr4.4:20659955-20659974 None:intergenic 30.0%
AGGATCTAATTATATGGGTA+TGG - chr4.4:20659875-20659894 MS.gene35037:CDS 30.0%
CAAAAGGAAATCCAAGTTAT+TGG + chr4.4:20659497-20659516 None:intergenic 30.0%
CAAAATAAACTCATGTTTGC+TGG + chr4.4:20658177-20658196 None:intergenic 30.0%
CATATAATTAGATCCTAGTG+AGG + chr4.4:20659871-20659890 None:intergenic 30.0%
CCTATAAAAGTTATAGGAGT+GGG + chr4.4:20658933-20658952 None:intergenic 30.0%
CTACAAACTTGAAATTGTAG+CGG - chr4.4:20659656-20659675 MS.gene35037:intron 30.0%
CTCACTAGGATCTAATTATA+TGG - chr4.4:20659869-20659888 MS.gene35037:CDS 30.0%
TAAAATCTGTATGTTCTACC+AGG + chr4.4:20660024-20660043 None:intergenic 30.0%
TAGAGAACAAAAACACTGTT+TGG + chr4.4:20660266-20660285 None:intergenic 30.0%
TGAAAACAAGCTATAGAATG+CGG - chr4.4:20657919-20657938 MS.gene35037:CDS 30.0%
TGCCAAAGCTATTAAATCAT+TGG - chr4.4:20658841-20658860 MS.gene35037:intron 30.0%
TGCCAATGATTTAATAGCTT+TGG + chr4.4:20658846-20658865 None:intergenic 30.0%
TGCTTGAAAATTGTGACAAA+AGG + chr4.4:20659513-20659532 None:intergenic 30.0%
TGTCGCAAAATTATTGTAGA+CGG + chr4.4:20658660-20658679 None:intergenic 30.0%
TGTTCCTACAATCAAGTAAA+TGG - chr4.4:20657747-20657766 MS.gene35037:intron 30.0%
TGTTTGTAATGATTTGAGTG+TGG - chr4.4:20660386-20660405 MS.gene35037:CDS 30.0%
TTGGGATTCCAAAATATGAA+TGG - chr4.4:20657039-20657058 MS.gene35037:CDS 30.0%
TTGTTCACTGTAATTCTATC+CGG - chr4.4:20657972-20657991 MS.gene35037:CDS 30.0%
! ATAACACCTTTAAGAAGATC+AGG + chr4.4:20657792-20657811 None:intergenic 30.0%
! ATCAAGTGACTTACATTTTG+AGG + chr4.4:20660166-20660185 None:intergenic 30.0%
! ATGAAGCATATGTTTTCCTA+GGG + chr4.4:20660439-20660458 None:intergenic 30.0%
! CATATTTGCTAGCAAGTAAA+GGG + chr4.4:20657423-20657442 None:intergenic 30.0%
! CATTTTTCCAATGTTATACC+TGG - chr4.4:20657107-20657126 MS.gene35037:CDS 30.0%
! TAACACCTTTAAGAAGATCA+GGG + chr4.4:20657791-20657810 None:intergenic 30.0%
!! AACAAGTTCTAAAACTTTGC+AGG - chr4.4:20659012-20659031 MS.gene35037:intron 30.0%
!! AGCTAGTTAATGTGTTTTTG+AGG - chr4.4:20657614-20657633 MS.gene35037:intron 30.0%
!! ATTTCAAGTTTGTAGCAACT+TGG + chr4.4:20659651-20659670 None:intergenic 30.0%
!! GTTTTATGGCTATTTTGTGT+AGG - chr4.4:20657273-20657292 MS.gene35037:CDS 30.0%
AAAAGATCACAAGCATTCTG+TGG - chr4.4:20659355-20659374 MS.gene35037:intron 35.0%
AAAGAAGCTGCAATGAGAAT+AGG + chr4.4:20657152-20657171 None:intergenic 35.0%
AAAGATCACAAGCATTCTGT+GGG - chr4.4:20659356-20659375 MS.gene35037:intron 35.0%
ACATATTTAGGGATCCAAGA+TGG - chr4.4:20657368-20657387 MS.gene35037:intron 35.0%
ACATTGGAAAAATGAGTTCC+TGG + chr4.4:20657101-20657120 None:intergenic 35.0%
ACGGCTTACAACCTAAATAA+TGG - chr4.4:20659736-20659755 MS.gene35037:CDS 35.0%
AGAAGTTGCAAATGTAGCTA+AGG - chr4.4:20659269-20659288 MS.gene35037:intron 35.0%
AGAATTATTGGAACCTTGTG+GGG + chr4.4:20659782-20659801 None:intergenic 35.0%
AGGATTAACATACTTCTTCC+AGG - chr4.4:20659225-20659244 MS.gene35037:intron 35.0%
ATATTTAGGGATCCAAGATG+GGG - chr4.4:20657370-20657389 MS.gene35037:intron 35.0%
ATGTAGTTTACCTGCTAGAA+TGG + chr4.4:20658390-20658409 None:intergenic 35.0%
ATTGTAGACGGCATTGTTAA+TGG + chr4.4:20658648-20658667 None:intergenic 35.0%
CAAAAACTAATTCCTCAACC+CGG + chr4.4:20657994-20658013 None:intergenic 35.0%
CAAACAGAACAAAGAAACAG+AGG + chr4.4:20656812-20656831 None:intergenic 35.0%
CAATGACCAATATGAACATG+AGG - chr4.4:20659977-20659996 MS.gene35037:intron 35.0%
CATATTTAGGGATCCAAGAT+GGG - chr4.4:20657369-20657388 MS.gene35037:intron 35.0%
CATGAGCGAAACCATTATTT+AGG + chr4.4:20659750-20659769 None:intergenic 35.0%
CTCAACTTTCGAGCATAAAT+TGG - chr4.4:20660086-20660105 MS.gene35037:CDS 35.0%
CTCATGTTCATATTGGTCAT+TGG + chr4.4:20659979-20659998 None:intergenic 35.0%
CTTGTTTCAAACCATAGGAA+AGG - chr4.4:20657181-20657200 MS.gene35037:CDS 35.0%
GAAGTTGCAAATGTAGCTAA+GGG - chr4.4:20659270-20659289 MS.gene35037:intron 35.0%
GAGAATTATTGGAACCTTGT+GGG + chr4.4:20659783-20659802 None:intergenic 35.0%
GCCTATAAAAGTTATAGGAG+TGG + chr4.4:20658934-20658953 None:intergenic 35.0%
GGAAAACATATGCTTCATGT+AGG - chr4.4:20660441-20660460 MS.gene35037:CDS 35.0%
GGATTCCAAAATATGAATGG+TGG - chr4.4:20657042-20657061 MS.gene35037:CDS 35.0%
GTCGTTCAATCTTGATCTAA+TGG - chr4.4:20659623-20659642 MS.gene35037:intron 35.0%
GTGGCAAATGGAAACTAAAT+GGG - chr4.4:20657814-20657833 MS.gene35037:intron 35.0%
TATCATATCCTGCATGGTAA+TGG - chr4.4:20657216-20657235 MS.gene35037:CDS 35.0%
TATTTACACCATTACCATGC+AGG + chr4.4:20657227-20657246 None:intergenic 35.0%
TCAATCACACAGCTCTTAAA+AGG + chr4.4:20657705-20657724 None:intergenic 35.0%
TGAATGTGTGGAAATTGTGA+CGG - chr4.4:20659717-20659736 MS.gene35037:CDS 35.0%
TGAGAATTATTGGAACCTTG+TGG + chr4.4:20659784-20659803 None:intergenic 35.0%
TGTAAGTCACTTGATGTTGT+TGG - chr4.4:20660171-20660190 MS.gene35037:CDS 35.0%
TGTTCACTGTAATTCTATCC+GGG - chr4.4:20657973-20657992 MS.gene35037:CDS 35.0%
TTCAAACCATAGGAAAGGTA+AGG - chr4.4:20657186-20657205 MS.gene35037:CDS 35.0%
TTCTCTTATCATATCCTGCA+TGG - chr4.4:20657210-20657229 MS.gene35037:CDS 35.0%
TTGCTAAATGCATGAATGTG+TGG - chr4.4:20659705-20659724 MS.gene35037:CDS 35.0%
TTTAAGAGCTGTGTGATTGA+TGG - chr4.4:20657705-20657724 MS.gene35037:intron 35.0%
! ATACATTACCACAGCATTGA+AGG + chr4.4:20657572-20657591 None:intergenic 35.0%
! ATTGGTTAAAGCACCACAAT+TGG - chr4.4:20660104-20660123 MS.gene35037:CDS 35.0%
! CATGAAGCATATGTTTTCCT+AGG + chr4.4:20660440-20660459 None:intergenic 35.0%
! CATTTAGTTTCCATTTGCCA+CGG + chr4.4:20657815-20657834 None:intergenic 35.0%
! CCCACTCCTATAACTTTTAT+AGG - chr4.4:20658930-20658949 MS.gene35037:CDS 35.0%
! CTTTCCTATGGTTTGAAACA+AGG + chr4.4:20657183-20657202 None:intergenic 35.0%
! GCATATTTGCTAGCAAGTAA+AGG + chr4.4:20657424-20657443 None:intergenic 35.0%
! GTCAGTGAGATTGATTTACT+TGG + chr4.4:20659444-20659463 None:intergenic 35.0%
! TCAGACCACCATTCATATTT+TGG + chr4.4:20657050-20657069 None:intergenic 35.0%
! TTGAACTGTGGAAGTTTTCT+TGG - chr4.4:20658575-20658594 MS.gene35037:CDS 35.0%
!! TATGATGTTGATGACTGGAA+AGG - chr4.4:20657532-20657551 MS.gene35037:intron 35.0%
!! TTAATAGCTTTGGCATTCAG+AGG + chr4.4:20658836-20658855 None:intergenic 35.0%
!! TTATGGCTATTTTGTGTAGG+TGG - chr4.4:20657276-20657295 MS.gene35037:CDS 35.0%
AAAGGAACAGACACCAATTG+TGG + chr4.4:20660120-20660139 None:intergenic 40.0%
AAGCAACCTTATGGAAACCT+TGG - chr4.4:20658716-20658735 MS.gene35037:intron 40.0%
AAGGTTGACATTGCAAGAGA+AGG - chr4.4:20656977-20656996 MS.gene35037:CDS 40.0%
AATTATTGGAACCTTGTGGG+GGG + chr4.4:20659780-20659799 None:intergenic 40.0%
ACAGGATTGGATTTGAACTG+TGG - chr4.4:20658563-20658582 MS.gene35037:CDS 40.0%
ACCTCAGGATCAAGAACAAA+GGG - chr4.4:20660226-20660245 MS.gene35037:CDS 40.0%
ACCTTCGTAGTTTCCAATCA+TGG + chr4.4:20658900-20658919 None:intergenic 40.0%
ACTGTCTTCTCATTTGGAGA+TGG - chr4.4:20660054-20660073 MS.gene35037:intron 40.0%
AGAAATTGAGTGTGGATGCA+AGG - chr4.4:20656937-20656956 MS.gene35037:CDS 40.0%
ATCACACAGCTCTTAAAAGG+TGG + chr4.4:20657702-20657721 None:intergenic 40.0%
ATGCAGTAAGACGAAGTTGT+TGG - chr4.4:20659540-20659559 MS.gene35037:CDS 40.0%
ATGCGTCTAGGTTTCTTTGA+TGG - chr4.4:20658683-20658702 MS.gene35037:intron 40.0%
ATTCTTGCCATAATGTCTGG+AGG - chr4.4:20659300-20659319 MS.gene35037:intron 40.0%
CAAGAGCAATGCACAATGTA+GGG - chr4.4:20657311-20657330 MS.gene35037:CDS 40.0%
CATCAACAGCAGAATTCACA+AGG + chr4.4:20657010-20657029 None:intergenic 40.0%
CCATAGCTGATGTCATCTTT+GGG - chr4.4:20659409-20659428 MS.gene35037:intron 40.0%
CGTGGCAAATGGAAACTAAA+TGG - chr4.4:20657813-20657832 MS.gene35037:intron 40.0%
CTGCAACAAGAAATTGAGTG+TGG - chr4.4:20656929-20656948 MS.gene35037:CDS 40.0%
CTGTTGATGTAAGCAGACTT+GGG - chr4.4:20657021-20657040 MS.gene35037:CDS 40.0%
CTTCATGGAGTCTCTAACAT+TGG - chr4.4:20657074-20657093 MS.gene35037:CDS 40.0%
GAAATTGAGTGTGGATGCAA+GGG - chr4.4:20656938-20656957 MS.gene35037:CDS 40.0%
GAATTATTGGAACCTTGTGG+GGG + chr4.4:20659781-20659800 None:intergenic 40.0%
GACATTATGGCAAGAATCAC+AGG + chr4.4:20659297-20659316 None:intergenic 40.0%
GAGAAACCTTACCTTTCCTA+TGG + chr4.4:20657195-20657214 None:intergenic 40.0%
GAGAATAGGCATAGGAAAAC+TGG + chr4.4:20657138-20657157 None:intergenic 40.0%
GATCCTGAGGTGAATATCAA+AGG + chr4.4:20660217-20660236 None:intergenic 40.0%
GATTGTGGATACCATGTCAT+GGG + chr4.4:20659940-20659959 None:intergenic 40.0%
GGTCTACAACAAACTGATGA+TGG - chr4.4:20657460-20657479 MS.gene35037:intron 40.0%
GTGATTCTTGCCATAATGTC+TGG - chr4.4:20659297-20659316 MS.gene35037:intron 40.0%
TAGCCTTTGATATTCACCTC+AGG - chr4.4:20660211-20660230 MS.gene35037:CDS 40.0%
TGACAGCACCTTCTGTATAT+CGG + chr4.4:20658601-20658620 None:intergenic 40.0%
TGATTGTGGATACCATGTCA+TGG + chr4.4:20659941-20659960 None:intergenic 40.0%
TGGAGATTCCAACAAGCTTA+AGG - chr4.4:20657480-20657499 MS.gene35037:intron 40.0%
TTGACATTGCAAGAGAAGGT+TGG - chr4.4:20656981-20657000 MS.gene35037:CDS 40.0%
TTGCTAGCAAATATGCAGCA+GGG - chr4.4:20657428-20657447 MS.gene35037:intron 40.0%
TTTCCATAAGGTTGCTTGCT+AGG + chr4.4:20658713-20658732 None:intergenic 40.0%
TTTCTTGGCCGATATACAGA+AGG - chr4.4:20658590-20658609 MS.gene35037:CDS 40.0%
TTTGCGACATTAATGCGTCT+AGG - chr4.4:20658671-20658690 MS.gene35037:CDS 40.0%
TTTGGACCAAGGTTTCCATA+AGG + chr4.4:20658725-20658744 None:intergenic 40.0%
! AAAGCCAACAAGTGTTTCTG+AGG + chr4.4:20660312-20660331 None:intergenic 40.0%
! AGGTGTTCTGTTTTCAACAG+AGG + chr4.4:20656775-20656794 None:intergenic 40.0%
! GCTTCCTTGTTTCAAACCAT+AGG - chr4.4:20657176-20657195 MS.gene35037:CDS 40.0%
! TTCCATAAGGTTGCTTGCTA+GGG + chr4.4:20658712-20658731 None:intergenic 40.0%
! TTCGAGAAAGTACACTTGAC+AGG - chr4.4:20660330-20660349 MS.gene35037:CDS 40.0%
! TTTACCTGCTAGAATGGACT+TGG + chr4.4:20658384-20658403 None:intergenic 40.0%
! TTTTGTGTAGGTGGTTTCGA+CGG - chr4.4:20657285-20657304 MS.gene35037:CDS 40.0%
!! AATCCAGCATTGGCGAATTA+CGG - chr4.4:20656903-20656922 MS.gene35037:CDS 40.0%
!! ATTTGCACCGACTACAATCA+CGG + chr4.4:20659178-20659197 None:intergenic 40.0%
!! CAGCATATGATGTTGATGAC+TGG - chr4.4:20657527-20657546 MS.gene35037:intron 40.0%
!! CTTCTTAAAGGTGTTATCCG+TGG - chr4.4:20657795-20657814 MS.gene35037:intron 40.0%
!! GGTTGGCTGGTTTAACATAT+TGG - chr4.4:20657332-20657351 MS.gene35037:intron 40.0%
!! GTGTTGCTTAAAAACTGTGC+AGG - chr4.4:20658803-20658822 MS.gene35037:intron 40.0%
!! TTGAGTGTGGTTGATGAACT+TGG - chr4.4:20660399-20660418 MS.gene35037:CDS 40.0%
AATGCTACTAGGGCCATGAT+TGG - chr4.4:20658884-20658903 MS.gene35037:CDS 45.0%
AGCAAAGCTTGTGGGAACTA+AGG + chr4.4:20659079-20659098 None:intergenic 45.0%
AGCAATGCACAATGTAGGGT+TGG - chr4.4:20657315-20657334 MS.gene35037:CDS 45.0%
AGCAGCAACCTTAAGCTTGT+TGG + chr4.4:20657491-20657510 None:intergenic 45.0%
AGGCGAAAACTGCAGATGTT+TGG + chr4.4:20656869-20656888 None:intergenic 45.0%
ATAGGAAAACTGGTTGCTCC+AGG + chr4.4:20657128-20657147 None:intergenic 45.0%
ATATGCTCAGTGACCTCACT+AGG - chr4.4:20659855-20659874 MS.gene35037:CDS 45.0%
ATCACAAGCATTCTGTGGGT+TGG - chr4.4:20659360-20659379 MS.gene35037:intron 45.0%
ATCCGGCCTCATGTTCATAT+TGG + chr4.4:20659986-20660005 None:intergenic 45.0%
ATTCGCCAATGCTGGATTCT+TGG + chr4.4:20656901-20656920 None:intergenic 45.0%
CAAACGTGATCCTCAGCTAA+AGG + chr4.4:20660138-20660157 None:intergenic 45.0%
CACAGCTCTTAAAAGGTGGT+TGG + chr4.4:20657698-20657717 None:intergenic 45.0%
CACCTCAGGATCAAGAACAA+AGG - chr4.4:20660225-20660244 MS.gene35037:CDS 45.0%
CAGAGGAAAACAGAGACGTT+AGG + chr4.4:20656795-20656814 None:intergenic 45.0%
CCCAAAGATGACATCAGCTA+TGG + chr4.4:20659412-20659431 None:intergenic 45.0%
CGGTGCAAATCTTGCAATAG+AGG - chr4.4:20659188-20659207 MS.gene35037:intron 45.0%
CTGTAATTCTATCCGGGTTG+AGG - chr4.4:20657979-20657998 MS.gene35037:CDS 45.0%
CTTGCTAGCAAATATGCAGC+AGG - chr4.4:20657427-20657446 MS.gene35037:intron 45.0%
GACCAATATGAACATGAGGC+CGG - chr4.4:20659981-20660000 MS.gene35037:intron 45.0%
GATCTTGATCTAACGGCTTC+AGG - chr4.4:20659682-20659701 MS.gene35037:intron 45.0%
GATGCAACCGTGATTGTAGT+CGG - chr4.4:20659168-20659187 MS.gene35037:intron 45.0%
GCAAGAGCAATGCACAATGT+AGG - chr4.4:20657310-20657329 MS.gene35037:CDS 45.0%
GCAATGTCAACCTTCTCACA+AGG + chr4.4:20656971-20656990 None:intergenic 45.0%
GCCATAGCTGATGTCATCTT+TGG - chr4.4:20659408-20659427 MS.gene35037:intron 45.0%
GCCATGATTGGAAACTACGA+AGG - chr4.4:20658896-20658915 MS.gene35037:CDS 45.0%
GCCCAATGCTAATGCTACTA+GGG - chr4.4:20658874-20658893 MS.gene35037:CDS 45.0%
GCCCTAGTAGCATTAGCATT+GGG + chr4.4:20658878-20658897 None:intergenic 45.0%
GCGGTTCGATCTTGATCTAA+CGG - chr4.4:20659675-20659694 MS.gene35037:intron 45.0%
GCTGACCCTGATCTTCTTAA+AGG - chr4.4:20657783-20657802 MS.gene35037:intron 45.0%
GCTGCAATGAGAATAGGCAT+AGG + chr4.4:20657146-20657165 None:intergenic 45.0%
GCTGTTGATGTAAGCAGACT+TGG - chr4.4:20657020-20657039 MS.gene35037:CDS 45.0%
GGGGAAACTGTCTTCTCATT+TGG - chr4.4:20660048-20660067 MS.gene35037:intron 45.0%
GGTTGCTCCAGGTATAACAT+TGG + chr4.4:20657117-20657136 None:intergenic 45.0%
GTGGAAGATTACCCATGACA+TGG - chr4.4:20659926-20659945 MS.gene35037:CDS 45.0%
TATGCAGCAGGGTATGTGAA+AGG - chr4.4:20657439-20657458 MS.gene35037:intron 45.0%
TCCCTTTGTTCTTGATCCTG+AGG + chr4.4:20660230-20660249 None:intergenic 45.0%
TGCACCTCAGAAACACTTGT+TGG - chr4.4:20660305-20660324 MS.gene35037:CDS 45.0%
TGTTGCCAAGAATCCAGCAT+TGG - chr4.4:20656893-20656912 MS.gene35037:CDS 45.0%
TTGTGGGAACTAAGGCTGTT+AGG + chr4.4:20659071-20659090 None:intergenic 45.0%
! AAGGTGTTATCCGTGGCAAA+TGG - chr4.4:20657802-20657821 MS.gene35037:intron 45.0%
! GACAGGAAAATCAGAAGCAC+TGG - chr4.4:20660347-20660366 MS.gene35037:CDS 45.0%
! GCATTGGTGCATTGAACATC+AGG + chr4.4:20659111-20659130 None:intergenic 45.0%
! GCTGAAGTGCAGACATCTTT+TGG + chr4.4:20658743-20658762 None:intergenic 45.0%
! TGCAGACATCTTTTGGACCA+AGG + chr4.4:20658736-20658755 None:intergenic 45.0%
! TGCTGAGGCTGCAATCTTTT+TGG + chr4.4:20659148-20659167 None:intergenic 45.0%
! TTTGCCACACTTTTGCTGAG+TGG - chr4.4:20656828-20656847 MS.gene35037:CDS 45.0%
!! ATCCAGCATTGGCGAATTAC+GGG - chr4.4:20656904-20656923 MS.gene35037:CDS 45.0%
AACCCGTAATTCGCCAATGC+TGG + chr4.4:20656909-20656928 None:intergenic 50.0%
AAGGCTGTTAGGCCTTGCAA+AGG + chr4.4:20659060-20659079 None:intergenic 50.0%
AATCACGGTTGCATCTGCTG+AGG + chr4.4:20659163-20659182 None:intergenic 50.0%
ACATTACACCAAGACCCCTG+AGG - chr4.4:20658349-20658368 MS.gene35037:CDS 50.0%
ACCACCACTCAGCAAAAGTG+TGG + chr4.4:20656835-20656854 None:intergenic 50.0%
ACTAACTGTTGCTGCTGTCC+TGG + chr4.4:20659246-20659265 None:intergenic 50.0%
AGATCAGGGTCAGCACAAGT+CGG + chr4.4:20657777-20657796 None:intergenic 50.0%
CATAATGTCTGGAGGAGGCA+TGG - chr4.4:20659308-20659327 MS.gene35037:intron 50.0%
CATGAAAATTGCCCCCCACA+AGG - chr4.4:20659766-20659785 MS.gene35037:CDS 50.0%
CATGCCTCCTCCAGACATTA+TGG + chr4.4:20659310-20659329 None:intergenic 50.0%
CTTGCCATAATGTCTGGAGG+AGG - chr4.4:20659303-20659322 MS.gene35037:intron 50.0%
GACCCTAGCAAGCAACCTTA+TGG - chr4.4:20658707-20658726 MS.gene35037:intron 50.0%
GCAAGTAAAGGGTCTTCTCC+TGG + chr4.4:20657412-20657431 None:intergenic 50.0%
GCGTTACACCTTCAATGCTG+TGG - chr4.4:20657561-20657580 MS.gene35037:intron 50.0%
GCTGTCAAGCAAGGGCTTAT+AGG - chr4.4:20658614-20658633 MS.gene35037:CDS 50.0%
GGAAGAGGTCAAGAAACACC+AGG - chr4.4:20657391-20657410 MS.gene35037:intron 50.0%
GGAGTCTCTAACATTGGTCC+AGG - chr4.4:20657080-20657099 MS.gene35037:CDS 50.0%
GGCAACAGAAAAGTTGCCCT+AGG - chr4.4:20660420-20660439 MS.gene35037:CDS 50.0%
GGCCCAATGCTAATGCTACT+AGG - chr4.4:20658873-20658892 MS.gene35037:CDS 50.0%
GGCCCTAGTAGCATTAGCAT+TGG + chr4.4:20658879-20658898 None:intergenic 50.0%
GGGTGAAAGACCTTGTGAGA+AGG - chr4.4:20656958-20656977 MS.gene35037:CDS 50.0%
GGTGTCTGTTCCTTTAGCTG+AGG - chr4.4:20660125-20660144 MS.gene35037:CDS 50.0%
TAGAGGCAGAGAGTCATGAC+AGG - chr4.4:20659205-20659224 MS.gene35037:intron 50.0%
TCTTGACCTCTTCCCCATCT+TGG + chr4.4:20657385-20657404 None:intergenic 50.0%
TGGATTCTTGGCAACATCGC+AGG + chr4.4:20656889-20656908 None:intergenic 50.0%
TGTCTGCACTTCAGCAAACC+AGG - chr4.4:20658748-20658767 MS.gene35037:intron 50.0%
! ATGCACAATGTAGGGTTGGC+TGG - chr4.4:20657319-20657338 MS.gene35037:CDS 50.0%
! TGGTGGTCTGAAGCACTTCA+TGG - chr4.4:20657059-20657078 MS.gene35037:CDS 50.0%
!! ACAGAAGGTGCTGTCAAGCA+AGG - chr4.4:20658605-20658624 MS.gene35037:CDS 50.0%
!! ATTCTGTGGGTTGGCTATCC+TGG - chr4.4:20659369-20659388 MS.gene35037:intron 50.0%
!! CAGAAGGTGCTGTCAAGCAA+GGG - chr4.4:20658606-20658625 MS.gene35037:CDS 50.0%
ACAGCCAGCAGCAAAGCTTG+TGG + chr4.4:20659088-20659107 None:intergenic 55.0%
ACTTGGCTGCAGCTTCCTCA+GGG + chr4.4:20658367-20658386 None:intergenic 55.0%
AGCTAGCTCGTGAAGCTGCA+AGG - chr4.4:20658771-20658790 MS.gene35037:intron 55.0%
AGGGATCCAAGATGGGGAAG+AGG - chr4.4:20657376-20657395 MS.gene35037:intron 55.0%
CAGCCAGCAGCAAAGCTTGT+GGG + chr4.4:20659087-20659106 None:intergenic 55.0%
CTCGTGAAGCTGCAAGGCAA+GGG - chr4.4:20658777-20658796 MS.gene35037:intron 55.0%
GCAGCCAAGTCCATTCTAGC+AGG - chr4.4:20658377-20658396 MS.gene35037:CDS 55.0%
GGCATCGTCTAGTGCAGCAT+TGG + chr4.4:20659127-20659146 None:intergenic 55.0%
GTTCCCACAAGCTTTGCTGC+TGG - chr4.4:20659081-20659100 MS.gene35037:intron 55.0%
GTTGGCTATCCTGGAGAAGC+TGG - chr4.4:20659378-20659397 MS.gene35037:intron 55.0%
TGAGGTGCATTGTGCACTGC+AGG + chr4.4:20660294-20660313 None:intergenic 55.0%
! GCCACACTTTTGCTGAGTGG+TGG - chr4.4:20656831-20656850 MS.gene35037:CDS 55.0%
ATGAGGCCGGATCCTGCTAC+AGG - chr4.4:20659994-20660013 MS.gene35037:intron 60.0%
CAGCTTCACGAGCTAGCTCC+TGG + chr4.4:20658769-20658788 None:intergenic 60.0%
CTACCAGGGTAGCCTGTAGC+AGG + chr4.4:20660009-20660028 None:intergenic 60.0%
CTTGGCTGCAGCTTCCTCAG+GGG + chr4.4:20658366-20658385 None:intergenic 60.0%
GACTTGGCTGCAGCTTCCTC+AGG + chr4.4:20658368-20658387 None:intergenic 60.0%
GATCCTGCTACAGGCTACCC+TGG - chr4.4:20660003-20660022 MS.gene35037:intron 60.0%
GCTCGTGAAGCTGCAAGGCA+AGG - chr4.4:20658776-20658795 MS.gene35037:intron 60.0%
GGCTATCCTGGAGAAGCTGG+TGG - chr4.4:20659381-20659400 MS.gene35037:intron 60.0%
GGGTAGCCTGTAGCAGGATC+CGG + chr4.4:20660003-20660022 None:intergenic 60.0%
GGTGCATTGTGCACTGCAGG+AGG + chr4.4:20660291-20660310 None:intergenic 60.0%
TGCAGCTTCCTCAGGGGTCT+TGG + chr4.4:20658360-20658379 None:intergenic 60.0%
GCAGCTCCACCAGCTTCTCC+AGG + chr4.4:20659390-20659409 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 20656768 20660497 20656768 ID=MS.gene35037
chr4.4 mRNA 20656768 20660497 20656768 ID=MS.gene35037.t1;Parent=MS.gene35037
chr4.4 exon 20660063 20660497 20660063 ID=MS.gene35037.t1.exon1;Parent=MS.gene35037.t1
chr4.4 CDS 20660063 20660497 20660063 ID=cds.MS.gene35037.t1;Parent=MS.gene35037.t1
chr4.4 exon 20659683 20659970 20659683 ID=MS.gene35037.t1.exon2;Parent=MS.gene35037.t1
chr4.4 CDS 20659683 20659970 20659683 ID=cds.MS.gene35037.t1;Parent=MS.gene35037.t1
chr4.4 exon 20659430 20659599 20659430 ID=MS.gene35037.t1.exon3;Parent=MS.gene35037.t1
chr4.4 CDS 20659430 20659599 20659430 ID=cds.MS.gene35037.t1;Parent=MS.gene35037.t1
chr4.4 exon 20658867 20658964 20658867 ID=MS.gene35037.t1.exon4;Parent=MS.gene35037.t1
chr4.4 CDS 20658867 20658964 20658867 ID=cds.MS.gene35037.t1;Parent=MS.gene35037.t1
chr4.4 exon 20658348 20658698 20658348 ID=MS.gene35037.t1.exon5;Parent=MS.gene35037.t1
chr4.4 CDS 20658348 20658698 20658348 ID=cds.MS.gene35037.t1;Parent=MS.gene35037.t1
chr4.4 exon 20657821 20658231 20657821 ID=MS.gene35037.t1.exon6;Parent=MS.gene35037.t1
chr4.4 CDS 20657821 20658231 20657821 ID=cds.MS.gene35037.t1;Parent=MS.gene35037.t1
chr4.4 exon 20656768 20657339 20656768 ID=MS.gene35037.t1.exon7;Parent=MS.gene35037.t1
chr4.4 CDS 20656768 20657339 20656768 ID=cds.MS.gene35037.t1;Parent=MS.gene35037.t1
Gene Sequence

>MS.gene35037

ATGGCCTCTGTTGAAAACAGAACACCTAACGTCTCTGTTTTCCTCTGTTTCTTTGTTCTGTTTGCCACACTTTTGCTGAGTGGTGGTCGAGTTTCGAGCCAAACATCTGCAGTTTTCGCCTGCGATGTTGCCAAGAATCCAGCATTGGCGAATTACGGGTTCTGCAACAAGAAATTGAGTGTGGATGCAAGGGTGAAAGACCTTGTGAGAAGGTTGACATTGCAAGAGAAGGTTGGTAACCTTGTGAATTCTGCTGTTGATGTAAGCAGACTTGGGATTCCAAAATATGAATGGTGGTCTGAAGCACTTCATGGAGTCTCTAACATTGGTCCAGGAACTCATTTTTCCAATGTTATACCTGGAGCAACCAGTTTTCCTATGCCTATTCTCATTGCAGCTTCTTTCAATGCTTCCTTGTTTCAAACCATAGGAAAGGTAAGGTTTCTCTTATCATATCCTGCATGGTAATGGTGTAAATAACATGTATTTTTAACTTTCAAATGATGTTTTATGGCTATTTTGTGTAGGTGGTTTCGACGGAAGCAAGAGCAATGCACAATGTAGGGTTGGCTGGTTTAACATATTGGTCACCAAACATAAACATATTTAGGGATCCAAGATGGGGAAGAGGTCAAGAAACACCAGGAGAAGACCCTTTACTTGCTAGCAAATATGCAGCAGGGTATGTGAAAGGTCTACAACAAACTGATGATGGAGATTCCAACAAGCTTAAGGTTGCTGCTTGTTGCAAACACTACACAGCATATGATGTTGATGACTGGAAAGGTGTTCAGCGTTACACCTTCAATGCTGTGGTAATGTATCAATATCATTGTTTGAAATGAAAGCTAGTTAATGTGTTTTTGAGGATTGTTTTAATTGTTGTTGATTTTGTTAGGTGACACAACAAGATTTAGATGACACATACCAACCACCTTTTAAGAGCTGTGTGATTGATGGAAATGTTGCTAGTGTTATGTGTTCCTACAATCAAGTAAATGGAAAACCGACTTGTGCTGACCCTGATCTTCTTAAAGGTGTTATCCGTGGCAAATGGAAACTAAATGGGTATCTTTTCATTTTTCAAACCTCTTAATTCTTTTATCTCACTCCATTCAAATATAAACAAAATATATTATCTCATATTTTTTTGAAAACAAGCTATAGAATGCGGCAACTTTAAAACTCTTGAGTGAGAACTTTGTTGTTCACTGTAATTCTATCCGGGTTGAGGAATTAGTTTTTGTAGTTGCGAACAAAGAATACCCAATTTACATCCAAAAAAATTATCTTTTATATGAGAATAAAAAAGATTGTTAGAAAATAAATATATATTAATTAAATGACATTTAGAAATAATATTATTAGATAGAGTGAAATCAAATAAGATTTATTTATATTTTAGACCAGCAAACATGAGTTTATTTTGCTTATATTGAAGATTTGAGAAAAATAATGATGTTTGCCATTCTTTTCTGTTTTCGATTTTTTTACAATAAATATGAATATGTTGTTTATCTATGTGCTATGCAGATATATAGTCTCTGATTGTGACTCAGTAGATGTGCTTTTCAAAAATCAACATTACACCAAGACCCCTGAGGAAGCTGCAGCCAAGTCCATTCTAGCAGGTAAACTACATTCGAAAAGATTACACAATCACATATTCATAGGCAACAACAGTTGAATTGTAATTTTATTTAGATGATTCATTTGAAATTTAATCAGTGTAGCTTCTTTAGAATTATACAAGGCAACTATTTATTTATCATTTTTGTGTACAAATTTTGATTTTACAGGATTGGATTTGAACTGTGGAAGTTTTCTTGGCCGATATACAGAAGGTGCTGTCAAGCAAGGGCTTATAGGTGAAGCATCCATTAACAATGCCGTCTACAATAATTTTGCGACATTAATGCGTCTAGGTTTCTTTGATGGCGACCCTAGCAAGCAACCTTATGGAAACCTTGGTCCAAAAGATGTCTGCACTTCAGCAAACCAGGAGCTAGCTCGTGAAGCTGCAAGGCAAGGGATTGTGTTGCTTAAAAACTGTGCAGGATCATTGCCTCTGAATGCCAAAGCTATTAAATCATTGGCAGTTATTGGGCCCAATGCTAATGCTACTAGGGCCATGATTGGAAACTACGAAGGTATCAAATTTTCCCACTCCTATAACTTTTATAGGCAAAATGTATGATATAGTTTGTTTTTGAAGTTGAATTGACATAATATTTAAGAAATACTAACAAGTTCTAAAACTTTGCAGGCATTCCATGCAAATATACATCACCTTTGCAAGGCCTAACAGCCTTAGTTCCCACAAGCTTTGCTGCTGGCTGTCCTGATGTTCAATGCACCAATGCTGCACTAGACGATGCCAAAAAGATTGCAGCCTCAGCAGATGCAACCGTGATTGTAGTCGGTGCAAATCTTGCAATAGAGGCAGAGAGTCATGACAGGATTAACATACTTCTTCCAGGACAGCAGCAACAGTTAGTTACAGAAGTTGCAAATGTAGCTAAGGGACCTGTGATTCTTGCCATAATGTCTGGAGGAGGCATGGATGTTTCCTTTGCTAAAACTAATAAAAAGATCACAAGCATTCTGTGGGTTGGCTATCCTGGAGAAGCTGGTGGAGCTGCCATAGCTGATGTCATCTTTGGGTATCATAATCCAAGTAAATCAATCTCACTGACATTCTTTCCTCATATTACAGAATTATCATTCCAATAACTTGGATTTCCTTTTGTCACAATTTTCAAGCACTCACGCATGCAGTAAGACGAAGTTGTTGGATCAAACAACTTTGATTTTAAACTCGGTATATCAGCTTCAAAAACAAACTTGAAATTATAGTCGTTCAATCTTGATCTAATGGCTCCAAGTTGCTACAAACTTGAAATTGTAGCGGTTCGATCTTGATCTAACGGCTTCAGGTTGCTAAATGCATGAATGTGTGGAAATTGTGACGGCTTACAACCTAAATAATGGTTTCGCTCATGAAAATTGCCCCCCACAAGGTTCCAATAATTCTCAATAATATATGTTTATATATTAGCTGCATTAGCATATACACTTGTATATATTATATGCTCAGTGACCTCACTAGGATCTAATTATATGGGTATGGTCTGCACAACTTAATTTTTTTTATTCAGGTGGAAGATTACCCATGACATGGTATCCACAATCATATGTAGACAAAGTACCAATGACCAATATGAACATGAGGCCGGATCCTGCTACAGGCTACCCTGGTAGAACATACAGATTTTATAAAGGGGAAACTGTCTTCTCATTTGGAGATGGAATAAGCTACTCAACTTTCGAGCATAAATTGGTTAAAGCACCACAATTGGTGTCTGTTCCTTTAGCTGAGGATCACGTTTGTCGTTCCTCAAAATGTAAGTCACTTGATGTTGTTGGCGAACATTGTCAAAACTTAGCCTTTGATATTCACCTCAGGATCAAGAACAAAGGGAAAATGAGCAGTAGCCAAACAGTGTTTTTGTTCTCTACTCCTCCTGCAGTGCACAATGCACCTCAGAAACACTTGTTGGCTTTCGAGAAAGTACACTTGACAGGAAAATCAGAAGCACTGGTCAGTTTCAAAGTAGATGTTTGTAATGATTTGAGTGTGGTTGATGAACTTGGCAACAGAAAAGTTGCCCTAGGAAAACATATGCTTCATGTAGGAGACTTGAAGCATCCTTTGAGCGTGATGATTTAA
Protein sequence

>MS.gene35037.t1

MASVENRTPNVSVFLCFFVLFATLLLSGGRVSSQTSAVFACDVAKNPALANYGFCNKKLSVDARVKDLVRRLTLQEKVGNLVNSAVDVSRLGIPKYEWWSEALHGVSNIGPGTHFSNVIPGATSFPMPILIAASFNASLFQTIGKVVSTEARAMHNVGLAGLTYWSPNINIFRDPRWGRGQETPGEDPLLASKYAAGYVKGLQQTDDGDSNKLKVAACCKHYTAYDVDDWKGVQRYTFNAVVTQQDLDDTYQPPFKSCVIDGNVASVMCSYNQVNGKPTCADPDLLKGVIRGKWKLNGYIVSDCDSVDVLFKNQHYTKTPEEAAAKSILAGLDLNCGSFLGRYTEGAVKQGLIGEASINNAVYNNFATLMRLGFFDGDPSKQPYGNLGPKDVCTSANQELAREAARQGIVLLKNCAGSLPLNAKAIKSLAVIGPNANATRAMIGNYEGIPCKYTSPLQGLTALVPTSFAAGCPDVQCTNAALDDAKKIAASADATVIVVGANLAIEAESHDRINILLPGQQQQLVTEVANVAKGPVILAIMSGGGMDVSFAKTNKKITSILWVGYPGEAGGAAIADVIFGYHNPSGRLPMTWYPQSYVDKVPMTNMNMRPDPATGYPGRTYRFYKGETVFSFGDGISYSTFEHKLVKAPQLVSVPLAEDHVCRSSKCKSLDVVGEHCQNLAFDIHLRIKNKGKMSSSQTVFLFSTPPAVHNAPQKHLLAFEKVHLTGKSEALVSFKVDVCNDLSVVDELGNRKVALGKHMLHVGDLKHPLSVMI