AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028420.t1 MTR_4g094285 97.674 774 18 0 1 774 1 774 0.0 1567
MS.gene028420.t1 MTR_2g008240 84.258 775 121 1 1 774 1 775 0.0 1344
MS.gene028420.t1 MTR_1g087240 56.935 757 314 5 19 772 8 755 0.0 889
MS.gene028420.t1 MTR_7g109390 57.124 765 306 8 17 772 10 761 0.0 884
MS.gene028420.t1 MTR_2g034720 57.124 765 305 9 14 772 7 754 0.0 870
MS.gene028420.t1 MTR_1g106860 55.757 773 302 12 13 764 6 759 0.0 847
MS.gene028420.t1 MTR_5g030860 48.280 756 365 9 42 774 30 782 0.0 740
MS.gene028420.t1 MTR_5g062320 46.310 786 393 14 10 774 3 780 0.0 713
MS.gene028420.t1 MTR_3g074060 46.863 781 382 15 14 774 9 776 0.0 709
MS.gene028420.t1 MTR_5g062430 46.036 782 392 13 14 774 10 782 0.0 708
MS.gene028420.t1 MTR_5g062370 46.164 782 391 13 14 774 10 782 0.0 701
MS.gene028420.t1 MTR_5g062650 45.685 788 399 14 9 774 5 785 0.0 690
MS.gene028420.t1 MTR_5g062370 49.158 594 281 10 14 594 10 595 0.0 570
MS.gene028420.t1 MTR_6g028090 26.845 637 367 26 38 612 13 612 3.10e-41 160
MS.gene028420.t1 MTR_6g028120 27.543 639 335 27 51 612 6 593 9.64e-41 159
MS.gene028420.t1 MTR_6g028100 26.803 638 337 26 53 612 27 612 2.90e-40 157
MS.gene028420.t1 MTR_3g079750 27.885 624 355 25 50 612 23 612 6.72e-39 154
MS.gene028420.t1 MTR_6g028110 25.849 677 382 27 38 641 14 643 8.78e-39 154
MS.gene028420.t1 MTR_6g028110 26.114 651 354 27 38 612 14 613 2.60e-38 152
MS.gene028420.t1 MTR_7g086030 25.496 655 358 26 53 637 3 597 1.20e-34 140
MS.gene028420.t1 MTR_7g086030 25.496 655 358 26 53 637 28 622 1.75e-34 140
MS.gene028420.t1 MTR_2g030000 27.365 687 356 30 43 648 19 643 2.00e-34 140
MS.gene028420.t1 MTR_3g462820 25.828 604 348 23 54 593 22 589 3.98e-34 139
MS.gene028420.t1 MTR_5g069800 26.911 628 352 27 52 612 5 592 8.51e-33 135
MS.gene028420.t1 MTR_5g069740 25.563 622 354 22 54 612 5 580 7.11e-32 132
MS.gene028420.t1 MTR_7g086010 27.989 368 213 16 89 437 118 452 1.40e-24 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028420.t1 AT5G64570 70.716 782 210 4 5 774 10 784 0.0 1177
MS.gene028420.t1 AT5G09730 67.839 768 235 3 12 774 13 773 0.0 1117
MS.gene028420.t1 AT5G64570 72.249 627 167 2 154 774 1 626 0.0 986
MS.gene028420.t1 AT5G64570 72.881 590 147 3 5 588 10 592 0.0 894
MS.gene028420.t1 AT3G19620 55.878 757 320 7 18 764 6 758 0.0 874
MS.gene028420.t1 AT5G49360 57.592 764 306 10 19 772 15 770 0.0 863
MS.gene028420.t1 AT1G02640 54.282 759 340 4 15 772 6 758 0.0 859
MS.gene028420.t1 AT5G10560 47.656 768 361 9 39 772 30 790 0.0 738
MS.gene028420.t1 AT1G78060 47.340 752 370 11 40 774 25 767 0.0 724
MS.gene028420.t1 AT5G09700 65.768 482 159 2 299 774 45 526 0.0 679
MS.gene028420.t1 AT3G47000 26.849 622 356 21 54 612 10 595 7.74e-39 153
MS.gene028420.t1 AT3G47050 26.777 605 364 22 51 603 7 584 9.84e-38 150
MS.gene028420.t1 AT3G47010 25.926 621 371 22 50 612 7 596 1.15e-35 144
MS.gene028420.t1 AT3G47040 24.580 655 375 24 54 643 10 610 4.78e-35 142
MS.gene028420.t1 AT3G47010 25.645 620 373 21 50 612 7 595 5.58e-35 141
MS.gene028420.t1 AT5G04885 27.074 639 339 28 54 616 30 617 6.51e-35 142
MS.gene028420.t1 AT5G04885 27.074 639 339 28 54 616 30 617 6.51e-35 142
MS.gene028420.t1 AT5G04885 27.074 639 339 28 54 616 47 634 8.37e-35 142
MS.gene028420.t1 AT3G62710 25.877 684 365 28 17 612 11 640 2.32e-34 140
MS.gene028420.t1 AT5G20940 27.044 636 360 27 41 612 18 613 3.23e-34 139
MS.gene028420.t1 AT3G47040 24.030 670 385 25 54 643 10 635 2.57e-32 134
MS.gene028420.t1 AT5G20950 26.187 653 376 28 38 625 90 701 2.66e-32 134
MS.gene028420.t1 AT5G20950 26.187 653 376 28 38 625 12 623 2.87e-32 133
MS.gene028420.t1 AT5G20950 26.187 653 376 28 38 625 12 623 2.87e-32 133
MS.gene028420.t1 AT3G47000 29.148 446 224 18 54 437 10 425 1.51e-31 129
MS.gene028420.t1 AT3G47040 26.786 448 232 18 54 437 10 425 6.36e-30 124
MS.gene028420.t1 AT3G47010 26.696 457 253 17 50 449 7 438 5.07e-28 118
MS.gene028420.t1 AT3G47040 22.907 681 387 26 54 643 10 643 1.41e-23 106
MS.gene028420.t1 AT3G47050 28.239 301 161 10 51 308 7 295 4.51e-23 102
MS.gene028420.t1 AT3G47010 26.392 413 265 15 215 612 29 417 8.84e-22 99.4
MS.gene028420.t1 AT3G47000 26.225 408 273 14 215 612 29 418 3.72e-21 97.4

Find 189 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGACCACCATTCATATTT+TGG 0.140699 4.2:+18835445 None:intergenic
CACCAAACATTAACATATTT+AGG 0.157012 4.2:-18835153 MS.gene028420:CDS
ATTAAATCATTGGCAGTTAT+TGG 0.184597 4.2:-18833660 MS.gene028420:CDS
CGTGGCAAATGGAAACTAAA+TGG 0.204423 4.2:-18834696 MS.gene028420:CDS
CTCAACTTTCGAGCATAAAT+TGG 0.211723 4.2:-18832424 MS.gene028420:CDS
GCTGACCCTGATCTTCTTAA+AGG 0.226486 4.2:-18834726 MS.gene028420:CDS
TGTTCCTACAATCAAGTAAA+TGG 0.245995 4.2:-18834762 MS.gene028420:CDS
GCTGAAGTGCAGACATCTTT+TGG 0.254948 4.2:+18833771 None:intergenic
ATCCAGCATTGGCGAATTAC+AGG 0.265672 4.2:-18835588 MS.gene028420:CDS
TCTTGACCTCTTCCCCATCT+TGG 0.278328 4.2:+18835127 None:intergenic
TGCAGCTTCCTCAGGGGTCT+TGG 0.289885 4.2:+18834154 None:intergenic
GCCATAGCTGATGTCATCTT+TGG 0.300033 4.2:-18833103 MS.gene028420:CDS
TGCCAATGATTTAATAGCTT+TGG 0.304831 4.2:+18833668 None:intergenic
CATGCCTCCTCCAGACATTA+TGG 0.305665 4.2:+18833204 None:intergenic
GACTTGGCTGCAGCTTCCTC+AGG 0.311461 4.2:+18834146 None:intergenic
GGGGAAACTGTCTTCTCATT+TGG 0.319056 4.2:-18832462 MS.gene028420:CDS
ATTCGCCAATGCTGGATTCT+TGG 0.323855 4.2:+18835594 None:intergenic
TCAATCACACAGCTCTTAAA+AGG 0.325703 4.2:+18834807 None:intergenic
ATTGTAGACGGCATTGTTAA+TGG 0.328849 4.2:+18833866 None:intergenic
ACCAAACATTAACATATTTA+GGG 0.344637 4.2:-18835152 MS.gene028420:CDS
TTAAATCATTGGCAGTTATT+GGG 0.348888 4.2:-18833659 MS.gene028420:CDS
TTTAAGAGCTGTGTGATTGA+TGG 0.379593 4.2:-18834804 MS.gene028420:CDS
GAGAATAGGCATAGGAAAAC+TGG 0.380578 4.2:+18835357 None:intergenic
GACCCTAGCAAGCAACCTTA+TGG 0.387544 4.2:-18833804 MS.gene028420:CDS
GCTGTCAAGCAAGGGCTTAT+AGG 0.388682 4.2:-18833897 MS.gene028420:CDS
ATGCGTCTAGGTTTCTTTGA+TGG 0.391137 4.2:-18833828 MS.gene028420:CDS
ATTCTGTGGGTTGGCTATCC+TGG 0.392669 4.2:-18833142 MS.gene028420:CDS
TTTCCATAAGGTTGCTTGCT+AGG 0.400087 4.2:+18833801 None:intergenic
CCATAGCTGATGTCATCTTT+GGG 0.402935 4.2:-18833102 MS.gene028420:CDS
TGCCAAAGCTATTAAATCAT+TGG 0.406948 4.2:-18833670 MS.gene028420:CDS
AGCAGCAACCTTAAGCTTGT+TGG 0.411669 4.2:+18835021 None:intergenic
ACTAACTGTTGCTGCTGTCC+TGG 0.428145 4.2:+18833268 None:intergenic
ATAACACCTTTAAGAAGATC+AGG 0.432414 4.2:+18834720 None:intergenic
TGACAGCACCTTCTGTATAT+CGG 0.436285 4.2:+18833913 None:intergenic
ACCTTCGTAATTTCCAATCA+TGG 0.436792 4.2:+18833614 None:intergenic
CTTGTTAGCAAATATGCAGC+AGG 0.437373 4.2:-18835082 MS.gene028420:CDS
ATTGGTTAAAGCACCACAAT+TGG 0.441371 4.2:-18832406 MS.gene028420:CDS
ATACATTACCACAGCATTGA+AGG 0.443167 4.2:+18834940 None:intergenic
TGCACCTCAGAAACACTTGT+TGG 0.443221 4.2:-18832205 MS.gene028420:CDS
TTGTGGGAACTAAGGCTGTT+AGG 0.443745 4.2:+18833443 None:intergenic
AGGCGAAAACTGCAGATGTT+TGG 0.443791 4.2:+18835626 None:intergenic
GGCCCAATGCTAATGCTACT+AGG 0.444209 4.2:-18833638 MS.gene028420:CDS
TTCCATAAGGTTGCTTGCTA+GGG 0.446833 4.2:+18833802 None:intergenic
CTTCATGGAGTCTCTTACAT+TGG 0.446926 4.2:-18835418 MS.gene028420:CDS
GCATTGGTGCATTGAACATC+AGG 0.448023 4.2:+18833403 None:intergenic
CATATTTGCTAACAAGTAAA+GGG 0.449152 4.2:+18835089 None:intergenic
GTTGGCTATCCTGGAGAAGC+TGG 0.450796 4.2:-18833133 MS.gene028420:CDS
GGTTGGCTGGTTTAACATAT+TGG 0.452673 4.2:-18835177 MS.gene028420:CDS
CTCATGTTCATATTGGTCAT+TGG 0.453094 4.2:+18832534 None:intergenic
GCATATTTGCTAACAAGTAA+AGG 0.454563 4.2:+18835088 None:intergenic
GTTCCCACAAGCTTTGCTGC+TGG 0.455205 4.2:-18833430 MS.gene028420:CDS
ATGCACAACGTCGGGTTGGC+TGG 0.455328 4.2:-18835190 MS.gene028420:CDS
AACCTGTAATTCGCCAATGC+TGG 0.456897 4.2:+18835586 None:intergenic
TCCCTAAATATGTTAATGTT+TGG 0.457701 4.2:+18835151 None:intergenic
CAGCTTCACGAGCTAGCTCC+TGG 0.458313 4.2:+18833745 None:intergenic
CAAGAGCAATGCACAACGTC+GGG 0.459504 4.2:-18835198 MS.gene028420:CDS
AGGATTAACATACTTCTTCC+AGG 0.461097 4.2:-18833286 MS.gene028420:CDS
ACATATTTAGGGATCCAAGA+TGG 0.461963 4.2:-18835141 MS.gene028420:CDS
ATCCGGCCTCATGTTCATAT+TGG 0.462557 4.2:+18832527 None:intergenic
AAAGAAGCTGCAATGAGAAT+AGG 0.464509 4.2:+18835343 None:intergenic
TCCCTTTGTTCTTGATCCTG+AGG 0.465656 4.2:+18832283 None:intergenic
CGGTGCAAATCTTGCAATAG+AGG 0.465750 4.2:-18833323 MS.gene028420:CDS
GGGTAGCCTGTAGCAGGATC+CGG 0.467218 4.2:+18832510 None:intergenic
TGTAAGTCGCTTGATGTTGT+TGG 0.471371 4.2:-18832339 MS.gene028420:CDS
TGGAGACTCCAACAAGCTTA+AGG 0.472702 4.2:-18835029 MS.gene028420:CDS
CATATTTAGGGATCCAAGAT+GGG 0.473111 4.2:-18835140 MS.gene028420:CDS
TGAGGTGCATTGTGCACTGC+AGG 0.474909 4.2:+18832219 None:intergenic
GCTGTTGATGTAAGCAGACT+TGG 0.476218 4.2:-18835472 MS.gene028420:CDS
GAGAAACCTTACCTTTCCTA+TGG 0.476556 4.2:+18835300 None:intergenic
TGGTGGTCTGAAGCACTTCA+TGG 0.476761 4.2:-18835433 MS.gene028420:CDS
AGCAAAGCTTGTGGGAACTA+AGG 0.478113 4.2:+18833435 None:intergenic
ATGTAGTTTACCTGCTAGAA+TGG 0.479155 4.2:+18834124 None:intergenic
ACCTCAGGATCAAGAACAAA+GGG 0.481557 4.2:-18832284 MS.gene028420:CDS
GGAGTCTCTTACATTGGTCC+AGG 0.482956 4.2:-18835412 MS.gene028420:CDS
ATTTGCACCGACTACAATCA+TGG 0.486153 4.2:+18833336 None:intergenic
TTTGGACCAAGGTTTCCATA+AGG 0.489120 4.2:+18833789 None:intergenic
TTGAGTGTGGTTGATGAACT+TGG 0.490808 4.2:-18832111 MS.gene028420:CDS
AAGGTGTTATCCGTGGCAAA+TGG 0.492172 4.2:-18834707 MS.gene028420:CDS
CTTTCCTATGGTTTGAAACA+AGG 0.492728 4.2:+18835312 None:intergenic
GGCATCGTCTAGTGCAGCAT+TGG 0.492888 4.2:+18833387 None:intergenic
CTACCAGGGTAGCCTGTAGC+AGG 0.493617 4.2:+18832504 None:intergenic
ATGAGGCCGGATCCTGCTAC+AGG 0.493861 4.2:-18832516 MS.gene028420:CDS
CTTTAAATCATCACGCTCAA+AGG 0.498511 4.2:+18832033 None:intergenic
AATGCTACTAGGGCCATGAT+TGG 0.501422 4.2:-18833627 MS.gene028420:CDS
GGTCTACAACAAACTGATGA+TGG 0.502433 4.2:-18835049 MS.gene028420:CDS
TTCGAGAAAGTACACTTGAC+AGG 0.502478 4.2:-18832180 MS.gene028420:CDS
GTGATTCTTGCCATAATGTC+TGG 0.506442 4.2:-18833214 MS.gene028420:CDS
TAGCCTTTGATATTCACCTC+AGG 0.509602 4.2:-18832299 MS.gene028420:CDS
TGTCTGCACTTCAGCAAACC+AGG 0.511708 4.2:-18833763 MS.gene028420:CDS
AGAAGTTGCAAATGTAGCTA+AGG 0.511795 4.2:-18833242 MS.gene028420:CDS
GCTTCCTTGTTTCAAACCAT+AGG 0.512430 4.2:-18835316 MS.gene028420:CDS
TTTACCTGCTAGAATGGACT+TGG 0.512571 4.2:+18834130 None:intergenic
GTGGCAAATGGAAACTAAAT+GGG 0.513107 4.2:-18834695 MS.gene028420:intron
GCTCGTGAAGCTGCAAGGCA+AGG 0.513237 4.2:-18833735 MS.gene028420:CDS
ACTGTCTTCTCATTTGGAGA+TGG 0.513776 4.2:-18832456 MS.gene028420:CDS
GCAGCTCCACCAGCTTCTCC+AGG 0.518655 4.2:+18833124 None:intergenic
CTTGTTTCAAACCATAGGAA+AGG 0.519277 4.2:-18835311 MS.gene028420:intron
ATAGGAAAACTGGTTGCTCC+AGG 0.520085 4.2:+18835367 None:intergenic
ACTTGGCTGCAGCTTCCTCA+GGG 0.522958 4.2:+18834147 None:intergenic
TAAAATCTGTATGTTCTACC+AGG 0.524873 4.2:+18832489 None:intergenic
CAAACGTGATCCTCAGCTAA+AGG 0.526847 4.2:+18832375 None:intergenic
CTCGTGAAGCTGCAAGGCAA+GGG 0.532764 4.2:-18833734 MS.gene028420:CDS
GCCCTAGTAGCATTAGCATT+GGG 0.533958 4.2:+18833636 None:intergenic
TTGGGATTCCAAAATATGAA+TGG 0.535603 4.2:-18835453 MS.gene028420:CDS
ATCACAAGCATTCTGTGGGT+TGG 0.539372 4.2:-18833151 MS.gene028420:CDS
TGGATTCTTGGCAACATCGC+AGG 0.541711 4.2:+18835606 None:intergenic
GACAGGAAAATCAGAAGCAC+TGG 0.541961 4.2:-18832163 MS.gene028420:CDS
CAGCCAGCAGCAAAGCTTGT+GGG 0.542585 4.2:+18833427 None:intergenic
CAGAGGAAAACAGAGACGTT+AGG 0.545414 4.2:+18835700 None:intergenic
GGCTATCCTGGAGAAGCTGG+TGG 0.546561 4.2:-18833130 MS.gene028420:CDS
TGTCGCAAAATTATTGTAGA+CGG 0.546588 4.2:+18833854 None:intergenic
ACAAGTAAAGGGTCTTCTCC+TGG 0.552386 4.2:+18835100 None:intergenic
GGTGTCTGTTCCTTTAGCTG+AGG 0.552719 4.2:-18832385 MS.gene028420:CDS
AGCTAGCTCGTGAAGCTGCA+AGG 0.553456 4.2:-18833740 MS.gene028420:CDS
GGCCCTAGTAGCATTAGCAT+TGG 0.555454 4.2:+18833635 None:intergenic
ACAGAAGGTGCTGTCAAGCA+AGG 0.561917 4.2:-18833906 MS.gene028420:CDS
TTAATAGCTTTGGCATTCAG+AGG 0.562815 4.2:+18833678 None:intergenic
GCAGCCAAGTCCATTCTAGC+AGG 0.563938 4.2:-18834134 MS.gene028420:intron
AAAAGATCACAAGCATTCTG+TGG 0.568704 4.2:-18833156 MS.gene028420:CDS
GATCCTGAGGTGAATATCAA+AGG 0.569135 4.2:+18832296 None:intergenic
AGGGATCCAAGATGGGGAAG+AGG 0.570000 4.2:-18835133 MS.gene028420:CDS
AAGGTTGACATTGCAAGAGA+AGG 0.572395 4.2:-18835515 MS.gene028420:CDS
AGCAATGCACAACGTCGGGT+TGG 0.573033 4.2:-18835194 MS.gene028420:CDS
AAGCAACCTTATGGAAACCT+TGG 0.575163 4.2:-18833795 MS.gene028420:CDS
GCCCAATGCTAATGCTACTA+GGG 0.577253 4.2:-18833637 MS.gene028420:CDS
TTGACATTGCAAGAGAAGGT+TGG 0.577392 4.2:-18835511 MS.gene028420:CDS
CACAGCTCTTAAAAGGTGGT+TGG 0.578720 4.2:+18834814 None:intergenic
CAGAAGGTGCTGTCAAGCAA+GGG 0.579821 4.2:-18833905 MS.gene028420:CDS
GGAAAACATATGCTTCATGT+AGG 0.580882 4.2:-18832069 MS.gene028420:CDS
TTTGCGACATTAATGCGTCT+AGG 0.581567 4.2:-18833840 MS.gene028420:CDS
TTTCTTGACCGATATACAGA+AGG 0.581868 4.2:-18833921 MS.gene028420:CDS
CAGCATATGATGTTGATGAC+TGG 0.584387 4.2:-18834982 MS.gene028420:CDS
CACCTCAGGATCAAGAACAA+AGG 0.587124 4.2:-18832285 MS.gene028420:CDS
CATTTAGTTTCCATTTGCCA+CGG 0.587656 4.2:+18834697 None:intergenic
AAAGATCACAAGCATTCTGT+GGG 0.588555 4.2:-18833155 MS.gene028420:CDS
CCCAAAGATGACATCAGCTA+TGG 0.590854 4.2:+18833102 None:intergenic
GACCAATATGAACATGAGGC+CGG 0.590969 4.2:-18832529 MS.gene028420:CDS
AGAAATTGAGTGTGGATGCA+AGG 0.597818 4.2:-18835555 MS.gene028420:CDS
CTGTTGATGTAAGCAGACTT+GGG 0.597835 4.2:-18835471 MS.gene028420:CDS
TGTTGCCAAGAATCCAGCAT+TGG 0.599346 4.2:-18835599 MS.gene028420:CDS
GAAGTTGCAAATGTAGCTAA+GGG 0.599742 4.2:-18833241 MS.gene028420:CDS
TATGCAGCAGGGTACGTGAA+AGG 0.600652 4.2:-18835070 MS.gene028420:CDS
TAGAGGCAGAGAGTCATGAC+AGG 0.601562 4.2:-18833306 MS.gene028420:CDS
GGGTGAAAGACCTTGTGAGA+AGG 0.601719 4.2:-18835534 MS.gene028420:CDS
ACTTTGTCTACATATGATTG+TGG 0.603286 4.2:+18832558 None:intergenic
CATCAACAGCAGAATTCACA+AGG 0.603921 4.2:+18835485 None:intergenic
GGTTGCTCCAGGTATAACAT+TGG 0.604127 4.2:+18835378 None:intergenic
CTTCTTAAAGGTGTTATCCG+TGG 0.607358 4.2:-18834714 MS.gene028420:CDS
ACAGCCAGCAGCAAAGCTTG+TGG 0.607399 4.2:+18833426 None:intergenic
GCGTTACACCTTCAATGCTG+TGG 0.608802 4.2:-18834948 MS.gene028420:intron
CATAATGTCTGGAGGAGGCA+TGG 0.611672 4.2:-18833203 MS.gene028420:CDS
CTTGGCTGCAGCTTCCTCAG+GGG 0.612324 4.2:+18834148 None:intergenic
GAGTCTCTTACATTGGTCCA+GGG 0.612774 4.2:-18835411 MS.gene028420:CDS
CTTGCCATAATGTCTGGAGG+AGG 0.615660 4.2:-18833208 MS.gene028420:CDS
AAAGGTGATGTATATTTGCA+TGG 0.623811 4.2:+18833472 None:intergenic
AAAGCCAACAAGTGTTTCTG+AGG 0.625434 4.2:+18832201 None:intergenic
GAAATTGAGTGTGGATGCAA+GGG 0.626342 4.2:-18835554 MS.gene028420:CDS
GCAAGAGCAATGCACAACGT+CGG 0.629064 4.2:-18835199 MS.gene028420:CDS
TGTTTGTAATGATTTGAGTG+TGG 0.631283 4.2:-18832124 MS.gene028420:CDS
GGCAACAGAAAAGTTGCCCT+AGG 0.635515 4.2:-18832090 MS.gene028420:CDS
TGATTGTGGATACCATGTCA+TGG 0.635666 4.2:+18832572 None:intergenic
GCTGCAATGAGAATAGGCAT+AGG 0.636909 4.2:+18835349 None:intergenic
AAATATACATCACCTTTGCA+AGG 0.638543 4.2:-18833466 MS.gene028420:CDS
GATGCAACCATGATTGTAGT+CGG 0.639143 4.2:-18833343 MS.gene028420:CDS
ATCACACAGCTCTTAAAAGG+TGG 0.639170 4.2:+18834810 None:intergenic
AAAGGAACAGACACCAATTG+TGG 0.639642 4.2:+18832393 None:intergenic
ACAGGATTGGATTTGAACTG+TGG 0.643755 4.2:-18833948 MS.gene028420:intron
GATTGTGGATACCATGTCAT+GGG 0.644060 4.2:+18832573 None:intergenic
AAGGCTGTTAGGCCTTGCAA+AGG 0.647052 4.2:+18833454 None:intergenic
TAACACCTTTAAGAAGATCA+GGG 0.647148 4.2:+18834721 None:intergenic
GGATTCCAAAATATGAATGG+TGG 0.648983 4.2:-18835450 MS.gene028420:CDS
GATCCTGCTACAGGCTACCC+TGG 0.649738 4.2:-18832507 MS.gene028420:CDS
CAATGACCAATATGAACATG+AGG 0.652360 4.2:-18832533 MS.gene028420:CDS
AATCATGGTTGCATCTGCTG+AGG 0.654432 4.2:+18833351 None:intergenic
TTGTTAGCAAATATGCAGCA+GGG 0.654785 4.2:-18835081 MS.gene028420:CDS
GCAATGTCAACCTTCTCACA+AGG 0.660006 4.2:+18835524 None:intergenic
CAAACAGAACAAAGAAACAG+AGG 0.661188 4.2:+18835683 None:intergenic
ACCACCACTCAGCAAAAGTG+TGG 0.664666 4.2:+18835660 None:intergenic
AGATCAGGGTCAGCACAAGT+CGG 0.668758 4.2:+18834735 None:intergenic
AAAATCTGTATGTTCTACCA+GGG 0.669451 4.2:+18832490 None:intergenic
GGAAGAGGTCAAGAAACACC+AGG 0.670289 4.2:-18835118 MS.gene028420:CDS
ATATTTAGGGATCCAAGATG+GGG 0.672629 4.2:-18835139 MS.gene028420:CDS
GCCATGATTGGAAATTACGA+AGG 0.674380 4.2:-18833615 MS.gene028420:intron
GTGGAAGGCTACCCATGACA+TGG 0.674570 4.2:-18832584 MS.gene028420:CDS
TATGATGTTGATGACTGGAA+AGG 0.681867 4.2:-18834977 MS.gene028420:CDS
GGTGCATTGTGCACTGCAGG+AGG 0.684891 4.2:+18832222 None:intergenic
CTGCAACAAGAAATTGAGTG+TGG 0.685493 4.2:-18835563 MS.gene028420:CDS
ATTCTTGCCATAATGTCTGG+AGG 0.713271 4.2:-18833211 MS.gene028420:CDS
ACATTACACCAAGACCCCTG+AGG 0.763610 4.2:-18834162 MS.gene028420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAACAAATATTGTTTTA+TGG - chr4.2:18832509-18832528 MS.gene028420:CDS 10.0%
!!! AACTTAATTTTTTTTTATTC+AGG - chr4.2:18835153-18835172 MS.gene028420:CDS 10.0%
!!! ATATTTTGTTTATATTTGAA+TGG + chr4.2:18833132-18833151 None:intergenic 10.0%
!!! CATATAAAAGATAATTTTTT+TGG + chr4.2:18833296-18833315 None:intergenic 10.0%
!! CATATATTATTGAGAATTAT+TGG + chr4.2:18835042-18835061 None:intergenic 15.0%
!!! AATTTTTTTGGATGTAAATT+GGG + chr4.2:18833284-18833303 None:intergenic 15.0%
!!! TAATTTTTTTGGATGTAAAT+TGG + chr4.2:18833285-18833304 None:intergenic 15.0%
!!! TTAATTTTTTTTTATTCAGG+TGG - chr4.2:18835156-18835175 MS.gene028420:CDS 15.0%
!! ACCAAACATTAACATATTTA+GGG - chr4.2:18832607-18832626 MS.gene028420:intron 20.0%
!!! AAATTTTGATTTTACAGGAT+TGG - chr4.2:18833798-18833817 MS.gene028420:CDS 20.0%
!!! AACAACTTTGATTTTAAACT+CGG - chr4.2:18834815-18834834 MS.gene028420:CDS 20.0%
!!! AACATACAGATTTTATAAAG+GGG - chr4.2:18835278-18835297 MS.gene028420:intron 20.0%
!!! AGAACATACAGATTTTATAA+AGG - chr4.2:18835276-18835295 MS.gene028420:intron 20.0%
!!! ATTTTGCCTATAAAAGTTAT+AGG + chr4.2:18834187-18834206 None:intergenic 20.0%
!!! CTTTTTATTAGTTTTAGCAA+AGG + chr4.2:18834588-18834607 None:intergenic 20.0%
!!! GAACATACAGATTTTATAAA+GGG - chr4.2:18835277-18835296 MS.gene028420:intron 20.0%
!!! TAATTGTTGTTGATTTTGTT+AGG - chr4.2:18832894-18832913 MS.gene028420:intron 20.0%
!!! TGTACAAATTTTGATTTTAC+AGG - chr4.2:18833793-18833812 MS.gene028420:CDS 20.0%
! AAATCGAAAACAGAAAAGAA+TGG + chr4.2:18833484-18833503 None:intergenic 25.0%
! AATGACAATTCTGTAATATG+AGG + chr4.2:18834722-18834741 None:intergenic 25.0%
! AGTGAGATAAAAGAATTAAG+AGG + chr4.2:18833109-18833128 None:intergenic 25.0%
! ATTAAATCATTGGCAGTTAT+TGG - chr4.2:18834099-18834118 MS.gene028420:intron 25.0%
! CACCAAACATTAACATATTT+AGG - chr4.2:18832606-18832625 MS.gene028420:intron 25.0%
! TATCATATCATGCATGTTAA+TGG - chr4.2:18832483-18832502 MS.gene028420:CDS 25.0%
! TCACTAGGATCTAATTATAT+GGG - chr4.2:18835118-18835137 MS.gene028420:CDS 25.0%
! TCCCTAAATATGTTAATGTT+TGG + chr4.2:18832611-18832630 None:intergenic 25.0%
! TTAAATCATTGGCAGTTATT+GGG - chr4.2:18834100-18834119 MS.gene028420:intron 25.0%
! TTACACAATCACATATTCAT+AGG - chr4.2:18833667-18833686 MS.gene028420:CDS 25.0%
!! CATATTTGCTAACAAGTAAA+GGG + chr4.2:18832673-18832692 None:intergenic 25.0%
!! TTTTCCATTTACTTGATTGT+AGG + chr4.2:18833004-18833023 None:intergenic 25.0%
!!! AGCTTCTTTAGAATTATACA+AGG - chr4.2:18833747-18833766 MS.gene028420:CDS 25.0%
!!! TTTTTTTTTATTCAGGTGGA+AGG - chr4.2:18835160-18835179 MS.gene028420:CDS 25.0%
AAAATCTGTATGTTCTACCA+GGG + chr4.2:18835272-18835291 None:intergenic 30.0%
AAAGGTGATGTATATTTGCA+TGG + chr4.2:18834290-18834309 None:intergenic 30.0%
AAATATACATCACCTTTGCA+AGG - chr4.2:18834293-18834312 MS.gene028420:intron 30.0%
ACTTTGTCTACATATGATTG+TGG + chr4.2:18835204-18835223 None:intergenic 30.0%
AGGATCTAATTATATGGGTA+TGG - chr4.2:18835123-18835142 MS.gene028420:CDS 30.0%
CAAAAGGAAATCCAAGTTAT+TGG + chr4.2:18834745-18834764 None:intergenic 30.0%
CAAAATAAACTCATGTTTGC+TGG + chr4.2:18833425-18833444 None:intergenic 30.0%
CATATAATTAGATCCTAGTG+AGG + chr4.2:18835119-18835138 None:intergenic 30.0%
CCTATAAAAGTTATAGGAGT+GGG + chr4.2:18834181-18834200 None:intergenic 30.0%
CTCACTAGGATCTAATTATA+TGG - chr4.2:18835117-18835136 MS.gene028420:CDS 30.0%
GAATTGTCATTCCAATAACT+TGG - chr4.2:18834731-18834750 MS.gene028420:CDS 30.0%
TAAAATCTGTATGTTCTACC+AGG + chr4.2:18835273-18835292 None:intergenic 30.0%
TAGAGAACAAAAACACTGTT+TGG + chr4.2:18835515-18835534 None:intergenic 30.0%
TGAAAACAAGCTATAGAATG+CGG - chr4.2:18833169-18833188 MS.gene028420:CDS 30.0%
TGCCAAAGCTATTAAATCAT+TGG - chr4.2:18834089-18834108 MS.gene028420:intron 30.0%
TGCCAATGATTTAATAGCTT+TGG + chr4.2:18834094-18834113 None:intergenic 30.0%
TGCTTGAAAATTGTGACAAA+AGG + chr4.2:18834761-18834780 None:intergenic 30.0%
TGTCGCAAAATTATTGTAGA+CGG + chr4.2:18833908-18833927 None:intergenic 30.0%
TGTTCCTACAATCAAGTAAA+TGG - chr4.2:18832997-18833016 MS.gene028420:intron 30.0%
TGTTTGTAATGATTTGAGTG+TGG - chr4.2:18835635-18835654 MS.gene028420:CDS 30.0%
TTGGGATTCCAAAATATGAA+TGG - chr4.2:18832306-18832325 MS.gene028420:CDS 30.0%
TTGTTCACTGTAATTCTATC+CGG - chr4.2:18833220-18833239 MS.gene028420:CDS 30.0%
! ATAACACCTTTAAGAAGATC+AGG + chr4.2:18833042-18833061 None:intergenic 30.0%
! ATGAAGCATATGTTTTCCTA+GGG + chr4.2:18835688-18835707 None:intergenic 30.0%
! GCATATTTGCTAACAAGTAA+AGG + chr4.2:18832674-18832693 None:intergenic 30.0%
! TAACACCTTTAAGAAGATCA+GGG + chr4.2:18833041-18833060 None:intergenic 30.0%
!! AACAAGTTCTAAAACTTTGC+AGG - chr4.2:18834260-18834279 MS.gene028420:intron 30.0%
!! AGCTAGTTAATGTGTTTTTG+AGG - chr4.2:18832864-18832883 MS.gene028420:intron 30.0%
!! ATTTCAAGTTTGTAGCAACT+TGG + chr4.2:18834899-18834918 None:intergenic 30.0%
!! GTTTTATGGCTATTTTGTGT+AGG - chr4.2:18832523-18832542 MS.gene028420:CDS 30.0%
AAAAGATCACAAGCATTCTG+TGG - chr4.2:18834603-18834622 MS.gene028420:intron 35.0%
AAAGAAGCTGCAATGAGAAT+AGG + chr4.2:18832419-18832438 None:intergenic 35.0%
AAAGATCACAAGCATTCTGT+GGG - chr4.2:18834604-18834623 MS.gene028420:intron 35.0%
ACATATTTAGGGATCCAAGA+TGG - chr4.2:18832618-18832637 MS.gene028420:intron 35.0%
ACCTTCGTAATTTCCAATCA+TGG + chr4.2:18834148-18834167 None:intergenic 35.0%
ACGGCTTACAACCTAAATAA+TGG - chr4.2:18834984-18835003 MS.gene028420:CDS 35.0%
AGAAGTTGCAAATGTAGCTA+AGG - chr4.2:18834517-18834536 MS.gene028420:intron 35.0%
AGAATTATTGGAACCTTGTG+GGG + chr4.2:18835030-18835049 None:intergenic 35.0%
AGGATTAACATACTTCTTCC+AGG - chr4.2:18834473-18834492 MS.gene028420:intron 35.0%
ATATTTAGGGATCCAAGATG+GGG - chr4.2:18832620-18832639 MS.gene028420:intron 35.0%
ATGTAGTTTACCTGCTAGAA+TGG + chr4.2:18833638-18833657 None:intergenic 35.0%
ATTGTAGACGGCATTGTTAA+TGG + chr4.2:18833896-18833915 None:intergenic 35.0%
CAAAAACTAATTCCTCAACC+CGG + chr4.2:18833242-18833261 None:intergenic 35.0%
CAAACAGAACAAAGAAACAG+AGG + chr4.2:18832079-18832098 None:intergenic 35.0%
CAATGACCAATATGAACATG+AGG - chr4.2:18835226-18835245 MS.gene028420:intron 35.0%
CATATTTAGGGATCCAAGAT+GGG - chr4.2:18832619-18832638 MS.gene028420:intron 35.0%
CATGAGCGAAACCATTATTT+AGG + chr4.2:18834998-18835017 None:intergenic 35.0%
CTCAACTTTCGAGCATAAAT+TGG - chr4.2:18835335-18835354 MS.gene028420:CDS 35.0%
CTCATGTTCATATTGGTCAT+TGG + chr4.2:18835228-18835247 None:intergenic 35.0%
CTTGTTTCAAACCATAGGAA+AGG - chr4.2:18832448-18832467 MS.gene028420:CDS 35.0%
GAAGTTGCAAATGTAGCTAA+GGG - chr4.2:18834518-18834537 MS.gene028420:intron 35.0%
GAGAATTATTGGAACCTTGT+GGG + chr4.2:18835031-18835050 None:intergenic 35.0%
GCCTATAAAAGTTATAGGAG+TGG + chr4.2:18834182-18834201 None:intergenic 35.0%
GGAAAACATATGCTTCATGT+AGG - chr4.2:18835690-18835709 MS.gene028420:CDS 35.0%
GGATTCCAAAATATGAATGG+TGG - chr4.2:18832309-18832328 MS.gene028420:CDS 35.0%
GTCGTTCAATCTTGATCTAA+TGG - chr4.2:18834871-18834890 MS.gene028420:intron 35.0%
GTGGCAAATGGAAACTAAAT+GGG - chr4.2:18833064-18833083 MS.gene028420:intron 35.0%
TCAATCACACAGCTCTTAAA+AGG + chr4.2:18832955-18832974 None:intergenic 35.0%
TGAATGTGTGGAAATTGTGA+CGG - chr4.2:18834965-18834984 MS.gene028420:CDS 35.0%
TGAGAATTATTGGAACCTTG+TGG + chr4.2:18835032-18835051 None:intergenic 35.0%
TGTTCACTGTAATTCTATCC+GGG - chr4.2:18833221-18833240 MS.gene028420:CDS 35.0%
TTCAAACCATAGGAAAGGTA+AGG - chr4.2:18832453-18832472 MS.gene028420:CDS 35.0%
TTGCTAAATGCATGAATGTG+TGG - chr4.2:18834953-18834972 MS.gene028420:CDS 35.0%
TTGTTAGCAAATATGCAGCA+GGG - chr4.2:18832678-18832697 MS.gene028420:intron 35.0%
TTTAAGAGCTGTGTGATTGA+TGG - chr4.2:18832955-18832974 MS.gene028420:intron 35.0%
TTTCTTGACCGATATACAGA+AGG - chr4.2:18833838-18833857 MS.gene028420:CDS 35.0%
! ATACATTACCACAGCATTGA+AGG + chr4.2:18832822-18832841 None:intergenic 35.0%
! ATCAAGCGACTTACATTTTG+AGG + chr4.2:18835415-18835434 None:intergenic 35.0%
! ATTGGTTAAAGCACCACAAT+TGG - chr4.2:18835353-18835372 MS.gene028420:CDS 35.0%
! CATGAAGCATATGTTTTCCT+AGG + chr4.2:18835689-18835708 None:intergenic 35.0%
! CATTTAGTTTCCATTTGCCA+CGG + chr4.2:18833065-18833084 None:intergenic 35.0%
! CCCACTCCTATAACTTTTAT+AGG - chr4.2:18834178-18834197 MS.gene028420:CDS 35.0%
! CGTTTTTCCAATGTTATACC+TGG - chr4.2:18832374-18832393 MS.gene028420:CDS 35.0%
! CTTTCCTATGGTTTGAAACA+AGG + chr4.2:18832450-18832469 None:intergenic 35.0%
! TCAGACCACCATTCATATTT+TGG + chr4.2:18832317-18832336 None:intergenic 35.0%
!! TATGATGTTGATGACTGGAA+AGG - chr4.2:18832782-18832801 MS.gene028420:intron 35.0%
!! TTAATAGCTTTGGCATTCAG+AGG + chr4.2:18834084-18834103 None:intergenic 35.0%
!! TTATGGCTATTTTGTGTAGG+TGG - chr4.2:18832526-18832545 MS.gene028420:CDS 35.0%
AAAGGAACAGACACCAATTG+TGG + chr4.2:18835369-18835388 None:intergenic 40.0%
AAGCAACCTTATGGAAACCT+TGG - chr4.2:18833964-18833983 MS.gene028420:intron 40.0%
AAGGTTGACATTGCAAGAGA+AGG - chr4.2:18832244-18832263 MS.gene028420:CDS 40.0%
AATTATTGGAACCTTGTGGG+GGG + chr4.2:18835028-18835047 None:intergenic 40.0%
ACAGGATTGGATTTGAACTG+TGG - chr4.2:18833811-18833830 MS.gene028420:CDS 40.0%
ACCTCAGGATCAAGAACAAA+GGG - chr4.2:18835475-18835494 MS.gene028420:CDS 40.0%
ACTGTCTTCTCATTTGGAGA+TGG - chr4.2:18835303-18835322 MS.gene028420:intron 40.0%
AGAAATTGAGTGTGGATGCA+AGG - chr4.2:18832204-18832223 MS.gene028420:CDS 40.0%
ATCACACAGCTCTTAAAAGG+TGG + chr4.2:18832952-18832971 None:intergenic 40.0%
ATGCAGTAAGACGAAGTTGT+TGG - chr4.2:18834788-18834807 MS.gene028420:CDS 40.0%
ATGCGTCTAGGTTTCTTTGA+TGG - chr4.2:18833931-18833950 MS.gene028420:CDS 40.0%
ATTCTTGCCATAATGTCTGG+AGG - chr4.2:18834548-18834567 MS.gene028420:intron 40.0%
CATCAACAGCAGAATTCACA+AGG + chr4.2:18832277-18832296 None:intergenic 40.0%
CCATAGCTGATGTCATCTTT+GGG - chr4.2:18834657-18834676 MS.gene028420:intron 40.0%
CGTGGCAAATGGAAACTAAA+TGG - chr4.2:18833063-18833082 MS.gene028420:intron 40.0%
CTGCAACAAGAAATTGAGTG+TGG - chr4.2:18832196-18832215 MS.gene028420:CDS 40.0%
CTGTTGATGTAAGCAGACTT+GGG - chr4.2:18832288-18832307 MS.gene028420:CDS 40.0%
CTTCATGGAGTCTCTTACAT+TGG - chr4.2:18832341-18832360 MS.gene028420:CDS 40.0%
CTTGTTAGCAAATATGCAGC+AGG - chr4.2:18832677-18832696 MS.gene028420:intron 40.0%
GAAATTGAGTGTGGATGCAA+GGG - chr4.2:18832205-18832224 MS.gene028420:CDS 40.0%
GAATTATTGGAACCTTGTGG+GGG + chr4.2:18835029-18835048 None:intergenic 40.0%
GAGAAACCTTACCTTTCCTA+TGG + chr4.2:18832462-18832481 None:intergenic 40.0%
GAGAATAGGCATAGGAAAAC+TGG + chr4.2:18832405-18832424 None:intergenic 40.0%
GATCCTGAGGTGAATATCAA+AGG + chr4.2:18835466-18835485 None:intergenic 40.0%
GATCTTGATCTAATGGCTTC+AGG - chr4.2:18834930-18834949 MS.gene028420:intron 40.0%
GATGCAACCATGATTGTAGT+CGG - chr4.2:18834416-18834435 MS.gene028420:intron 40.0%
GATTGTGGATACCATGTCAT+GGG + chr4.2:18835189-18835208 None:intergenic 40.0%
GCCATGATTGGAAATTACGA+AGG - chr4.2:18834144-18834163 MS.gene028420:CDS 40.0%
GCCATTAGATCAAGATCGAA+CGG + chr4.2:18834927-18834946 None:intergenic 40.0%
GGTCTACAACAAACTGATGA+TGG - chr4.2:18832710-18832729 MS.gene028420:intron 40.0%
GTGATTCTTGCCATAATGTC+TGG - chr4.2:18834545-18834564 MS.gene028420:intron 40.0%
TAGCCTTTGATATTCACCTC+AGG - chr4.2:18835460-18835479 MS.gene028420:CDS 40.0%
TGACAGCACCTTCTGTATAT+CGG + chr4.2:18833849-18833868 None:intergenic 40.0%
TGATTGTGGATACCATGTCA+TGG + chr4.2:18835190-18835209 None:intergenic 40.0%
TGTAAGTCGCTTGATGTTGT+TGG - chr4.2:18835420-18835439 MS.gene028420:CDS 40.0%
TTGACATTGCAAGAGAAGGT+TGG - chr4.2:18832248-18832267 MS.gene028420:CDS 40.0%
TTTCCATAAGGTTGCTTGCT+AGG + chr4.2:18833961-18833980 None:intergenic 40.0%
TTTGCGACATTAATGCGTCT+AGG - chr4.2:18833919-18833938 MS.gene028420:CDS 40.0%
TTTGGACCAAGGTTTCCATA+AGG + chr4.2:18833973-18833992 None:intergenic 40.0%
! AAAGCCAACAAGTGTTTCTG+AGG + chr4.2:18835561-18835580 None:intergenic 40.0%
! AGGTGTTCTGTTTTCAACAG+AGG + chr4.2:18832042-18832061 None:intergenic 40.0%
! GCTTCCTTGTTTCAAACCAT+AGG - chr4.2:18832443-18832462 MS.gene028420:CDS 40.0%
! GTCAGTGAGATTGACTTACT+TGG + chr4.2:18834692-18834711 None:intergenic 40.0%
! TTCCATAAGGTTGCTTGCTA+GGG + chr4.2:18833960-18833979 None:intergenic 40.0%
! TTCGAGAAAGTACACTTGAC+AGG - chr4.2:18835579-18835598 MS.gene028420:CDS 40.0%
! TTTACCTGCTAGAATGGACT+TGG + chr4.2:18833632-18833651 None:intergenic 40.0%
! TTTTGTGTAGGTGGTTTCGA+CGG - chr4.2:18832535-18832554 MS.gene028420:CDS 40.0%
!! ATTTGCACCGACTACAATCA+TGG + chr4.2:18834426-18834445 None:intergenic 40.0%
!! CAGCATATGATGTTGATGAC+TGG - chr4.2:18832777-18832796 MS.gene028420:intron 40.0%
!! CTTCTTAAAGGTGTTATCCG+TGG - chr4.2:18833045-18833064 MS.gene028420:intron 40.0%
!! GGTTGGCTGGTTTAACATAT+TGG - chr4.2:18832582-18832601 MS.gene028420:CDS 40.0%
!! GTGTTGCTTAAAAACTGTGC+AGG - chr4.2:18834051-18834070 MS.gene028420:intron 40.0%
!! TTGAGTGTGGTTGATGAACT+TGG - chr4.2:18835648-18835667 MS.gene028420:CDS 40.0%
AACCTGTAATTCGCCAATGC+TGG + chr4.2:18832176-18832195 None:intergenic 45.0%
AATCATGGTTGCATCTGCTG+AGG + chr4.2:18834411-18834430 None:intergenic 45.0%
AATGCTACTAGGGCCATGAT+TGG - chr4.2:18834132-18834151 MS.gene028420:intron 45.0%
ACAAGTAAAGGGTCTTCTCC+TGG + chr4.2:18832662-18832681 None:intergenic 45.0%
ACATTGGAAAAACGAGTCCC+TGG + chr4.2:18832368-18832387 None:intergenic 45.0%
AGCAAAGCTTGTGGGAACTA+AGG + chr4.2:18834327-18834346 None:intergenic 45.0%
AGCAGCAACCTTAAGCTTGT+TGG + chr4.2:18832741-18832760 None:intergenic 45.0%
AGGCGAAAACTGCAGATGTT+TGG + chr4.2:18832136-18832155 None:intergenic 45.0%
ATAGGAAAACTGGTTGCTCC+AGG + chr4.2:18832395-18832414 None:intergenic 45.0%
ATATGCTCAGTGACCTCACT+AGG - chr4.2:18835103-18835122 MS.gene028420:CDS 45.0%
ATCACAAGCATTCTGTGGGT+TGG - chr4.2:18834608-18834627 MS.gene028420:intron 45.0%
ATCCGGCCTCATGTTCATAT+TGG + chr4.2:18835235-18835254 None:intergenic 45.0%
ATTCGCCAATGCTGGATTCT+TGG + chr4.2:18832168-18832187 None:intergenic 45.0%
CAAACGTGATCCTCAGCTAA+AGG + chr4.2:18835387-18835406 None:intergenic 45.0%
CACAGCTCTTAAAAGGTGGT+TGG + chr4.2:18832948-18832967 None:intergenic 45.0%
CACCTCAGGATCAAGAACAA+AGG - chr4.2:18835474-18835493 MS.gene028420:CDS 45.0%
CAGAGGAAAACAGAGACGTT+AGG + chr4.2:18832062-18832081 None:intergenic 45.0%
CCCAAAGATGACATCAGCTA+TGG + chr4.2:18834660-18834679 None:intergenic 45.0%
CGGTGCAAATCTTGCAATAG+AGG - chr4.2:18834436-18834455 MS.gene028420:intron 45.0%
CTGTAATTCTATCCGGGTTG+AGG - chr4.2:18833227-18833246 MS.gene028420:CDS 45.0%
GACCAATATGAACATGAGGC+CGG - chr4.2:18835230-18835249 MS.gene028420:intron 45.0%
GAGTCTCTTACATTGGTCCA+GGG - chr4.2:18832348-18832367 MS.gene028420:CDS 45.0%
GCAATGTCAACCTTCTCACA+AGG + chr4.2:18832238-18832257 None:intergenic 45.0%
GCCATAGCTGATGTCATCTT+TGG - chr4.2:18834656-18834675 MS.gene028420:intron 45.0%
GCCCAATGCTAATGCTACTA+GGG - chr4.2:18834122-18834141 MS.gene028420:intron 45.0%
GCCCTAGTAGCATTAGCATT+GGG + chr4.2:18834126-18834145 None:intergenic 45.0%
GCCGTTCGATCTTGATCTAA+TGG - chr4.2:18834923-18834942 MS.gene028420:intron 45.0%
GCTGACCCTGATCTTCTTAA+AGG - chr4.2:18833033-18833052 MS.gene028420:intron 45.0%
GCTGCAATGAGAATAGGCAT+AGG + chr4.2:18832413-18832432 None:intergenic 45.0%
GCTGTTGATGTAAGCAGACT+TGG - chr4.2:18832287-18832306 MS.gene028420:CDS 45.0%
GGGGAAACTGTCTTCTCATT+TGG - chr4.2:18835297-18835316 MS.gene028420:intron 45.0%
GGTTGCTCCAGGTATAACAT+TGG + chr4.2:18832384-18832403 None:intergenic 45.0%
TCCCTTTGTTCTTGATCCTG+AGG + chr4.2:18835479-18835498 None:intergenic 45.0%
TGCACCTCAGAAACACTTGT+TGG - chr4.2:18835554-18835573 MS.gene028420:CDS 45.0%
TGGAGACTCCAACAAGCTTA+AGG - chr4.2:18832730-18832749 MS.gene028420:intron 45.0%
TGTTGCCAAGAATCCAGCAT+TGG - chr4.2:18832160-18832179 MS.gene028420:CDS 45.0%
TTGTGGGAACTAAGGCTGTT+AGG + chr4.2:18834319-18834338 None:intergenic 45.0%
! AAGGTGTTATCCGTGGCAAA+TGG - chr4.2:18833052-18833071 MS.gene028420:intron 45.0%
! GACAGGAAAATCAGAAGCAC+TGG - chr4.2:18835596-18835615 MS.gene028420:CDS 45.0%
! GCATTGGTGCATTGAACATC+AGG + chr4.2:18834359-18834378 None:intergenic 45.0%
! GCTGAAGTGCAGACATCTTT+TGG + chr4.2:18833991-18834010 None:intergenic 45.0%
! TGCAGACATCTTTTGGACCA+AGG + chr4.2:18833984-18834003 None:intergenic 45.0%
! TGCTGAGGCTGCAATCTTTT+TGG + chr4.2:18834396-18834415 None:intergenic 45.0%
! TTTGCCACACTTTTGCTGAG+TGG - chr4.2:18832095-18832114 MS.gene028420:CDS 45.0%
!! ATCCAGCATTGGCGAATTAC+AGG - chr4.2:18832171-18832190 MS.gene028420:CDS 45.0%
AAGGCTGTTAGGCCTTGCAA+AGG + chr4.2:18834308-18834327 None:intergenic 50.0%
ACATTACACCAAGACCCCTG+AGG - chr4.2:18833597-18833616 MS.gene028420:intron 50.0%
ACCACCACTCAGCAAAAGTG+TGG + chr4.2:18832102-18832121 None:intergenic 50.0%
ACTAACTGTTGCTGCTGTCC+TGG + chr4.2:18834494-18834513 None:intergenic 50.0%
AGATCAGGGTCAGCACAAGT+CGG + chr4.2:18833027-18833046 None:intergenic 50.0%
CAAGAGCAATGCACAACGTC+GGG - chr4.2:18832561-18832580 MS.gene028420:CDS 50.0%
CATAATGTCTGGAGGAGGCA+TGG - chr4.2:18834556-18834575 MS.gene028420:intron 50.0%
CATGAAAATTGCCCCCCACA+AGG - chr4.2:18835014-18835033 MS.gene028420:CDS 50.0%
CATGCCTCCTCCAGACATTA+TGG + chr4.2:18834558-18834577 None:intergenic 50.0%
CTTGCCATAATGTCTGGAGG+AGG - chr4.2:18834551-18834570 MS.gene028420:intron 50.0%
GACCCTAGCAAGCAACCTTA+TGG - chr4.2:18833955-18833974 MS.gene028420:intron 50.0%
GCAAGAGCAATGCACAACGT+CGG - chr4.2:18832560-18832579 MS.gene028420:CDS 50.0%
GCGTTACACCTTCAATGCTG+TGG - chr4.2:18832811-18832830 MS.gene028420:intron 50.0%
GCTGTCAAGCAAGGGCTTAT+AGG - chr4.2:18833862-18833881 MS.gene028420:CDS 50.0%
GGAAGAGGTCAAGAAACACC+AGG - chr4.2:18832641-18832660 MS.gene028420:intron 50.0%
GGAGTCTCTTACATTGGTCC+AGG - chr4.2:18832347-18832366 MS.gene028420:CDS 50.0%
GGCAACAGAAAAGTTGCCCT+AGG - chr4.2:18835669-18835688 MS.gene028420:CDS 50.0%
GGCCCAATGCTAATGCTACT+AGG - chr4.2:18834121-18834140 MS.gene028420:intron 50.0%
GGCCCTAGTAGCATTAGCAT+TGG + chr4.2:18834127-18834146 None:intergenic 50.0%
GGGTGAAAGACCTTGTGAGA+AGG - chr4.2:18832225-18832244 MS.gene028420:CDS 50.0%
GGTGTCTGTTCCTTTAGCTG+AGG - chr4.2:18835374-18835393 MS.gene028420:CDS 50.0%
TAGAGGCAGAGAGTCATGAC+AGG - chr4.2:18834453-18834472 MS.gene028420:intron 50.0%
TATGCAGCAGGGTACGTGAA+AGG - chr4.2:18832689-18832708 MS.gene028420:intron 50.0%
TCTTGACCTCTTCCCCATCT+TGG + chr4.2:18832635-18832654 None:intergenic 50.0%
TGGATTCTTGGCAACATCGC+AGG + chr4.2:18832156-18832175 None:intergenic 50.0%
TGTCTGCACTTCAGCAAACC+AGG - chr4.2:18833996-18834015 MS.gene028420:intron 50.0%
! TGGTGGTCTGAAGCACTTCA+TGG - chr4.2:18832326-18832345 MS.gene028420:CDS 50.0%
!! ACAGAAGGTGCTGTCAAGCA+AGG - chr4.2:18833853-18833872 MS.gene028420:CDS 50.0%
!! ATTCTGTGGGTTGGCTATCC+TGG - chr4.2:18834617-18834636 MS.gene028420:intron 50.0%
!! CAGAAGGTGCTGTCAAGCAA+GGG - chr4.2:18833854-18833873 MS.gene028420:CDS 50.0%
ACAGCCAGCAGCAAAGCTTG+TGG + chr4.2:18834336-18834355 None:intergenic 55.0%
ACTTGGCTGCAGCTTCCTCA+GGG + chr4.2:18833615-18833634 None:intergenic 55.0%
AGCAATGCACAACGTCGGGT+TGG - chr4.2:18832565-18832584 MS.gene028420:CDS 55.0%
AGCTAGCTCGTGAAGCTGCA+AGG - chr4.2:18834019-18834038 MS.gene028420:intron 55.0%
AGGGATCCAAGATGGGGAAG+AGG - chr4.2:18832626-18832645 MS.gene028420:intron 55.0%
CAGCCAGCAGCAAAGCTTGT+GGG + chr4.2:18834335-18834354 None:intergenic 55.0%
CTCGTGAAGCTGCAAGGCAA+GGG - chr4.2:18834025-18834044 MS.gene028420:intron 55.0%
GCAGCCAAGTCCATTCTAGC+AGG - chr4.2:18833625-18833644 MS.gene028420:CDS 55.0%
GGCATCGTCTAGTGCAGCAT+TGG + chr4.2:18834375-18834394 None:intergenic 55.0%
GTGGAAGGCTACCCATGACA+TGG - chr4.2:18835175-18835194 MS.gene028420:CDS 55.0%
GTTCCCACAAGCTTTGCTGC+TGG - chr4.2:18834329-18834348 MS.gene028420:intron 55.0%
GTTGGCTATCCTGGAGAAGC+TGG - chr4.2:18834626-18834645 MS.gene028420:intron 55.0%
TGAGGTGCATTGTGCACTGC+AGG + chr4.2:18835543-18835562 None:intergenic 55.0%
! GCCACACTTTTGCTGAGTGG+TGG - chr4.2:18832098-18832117 MS.gene028420:CDS 55.0%
ATGAGGCCGGATCCTGCTAC+AGG - chr4.2:18835243-18835262 MS.gene028420:intron 60.0%
CAGCTTCACGAGCTAGCTCC+TGG + chr4.2:18834017-18834036 None:intergenic 60.0%
CTACCAGGGTAGCCTGTAGC+AGG + chr4.2:18835258-18835277 None:intergenic 60.0%
CTTGGCTGCAGCTTCCTCAG+GGG + chr4.2:18833614-18833633 None:intergenic 60.0%
GACTTGGCTGCAGCTTCCTC+AGG + chr4.2:18833616-18833635 None:intergenic 60.0%
GATCCTGCTACAGGCTACCC+TGG - chr4.2:18835252-18835271 MS.gene028420:intron 60.0%
GCTCGTGAAGCTGCAAGGCA+AGG - chr4.2:18834024-18834043 MS.gene028420:intron 60.0%
GGCTATCCTGGAGAAGCTGG+TGG - chr4.2:18834629-18834648 MS.gene028420:intron 60.0%
GGGTAGCCTGTAGCAGGATC+CGG + chr4.2:18835252-18835271 None:intergenic 60.0%
GGTGCATTGTGCACTGCAGG+AGG + chr4.2:18835540-18835559 None:intergenic 60.0%
TGCAGCTTCCTCAGGGGTCT+TGG + chr4.2:18833608-18833627 None:intergenic 60.0%
!! ATGCACAACGTCGGGTTGGC+TGG - chr4.2:18832569-18832588 MS.gene028420:CDS 60.0%
GCAGCTCCACCAGCTTCTCC+AGG + chr4.2:18834638-18834657 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 18832035 18835746 18832035 ID=MS.gene028420
chr4.2 mRNA 18832035 18835746 18832035 ID=MS.gene028420.t1;Parent=MS.gene028420
chr4.2 exon 18835312 18835746 18835312 ID=MS.gene028420.t1.exon1;Parent=MS.gene028420.t1
chr4.2 CDS 18835312 18835746 18835312 ID=cds.MS.gene028420.t1;Parent=MS.gene028420.t1
chr4.2 exon 18834949 18835236 18834949 ID=MS.gene028420.t1.exon2;Parent=MS.gene028420.t1
chr4.2 CDS 18834949 18835236 18834949 ID=cds.MS.gene028420.t1;Parent=MS.gene028420.t1
chr4.2 exon 18834696 18834865 18834696 ID=MS.gene028420.t1.exon3;Parent=MS.gene028420.t1
chr4.2 CDS 18834696 18834865 18834696 ID=cds.MS.gene028420.t1;Parent=MS.gene028420.t1
chr4.2 exon 18834135 18834232 18834135 ID=MS.gene028420.t1.exon4;Parent=MS.gene028420.t1
chr4.2 CDS 18834135 18834232 18834135 ID=cds.MS.gene028420.t1;Parent=MS.gene028420.t1
chr4.2 exon 18833616 18833966 18833616 ID=MS.gene028420.t1.exon5;Parent=MS.gene028420.t1
chr4.2 CDS 18833616 18833966 18833616 ID=cds.MS.gene028420.t1;Parent=MS.gene028420.t1
chr4.2 exon 18833089 18833499 18833089 ID=MS.gene028420.t1.exon6;Parent=MS.gene028420.t1
chr4.2 CDS 18833089 18833499 18833089 ID=cds.MS.gene028420.t1;Parent=MS.gene028420.t1
chr4.2 exon 18832035 18832606 18832035 ID=MS.gene028420.t1.exon7;Parent=MS.gene028420.t1
chr4.2 CDS 18832035 18832606 18832035 ID=cds.MS.gene028420.t1;Parent=MS.gene028420.t1
Gene Sequence

>MS.gene028420

ATGGCCTCTGTTGAAAACAGAACACCTAACGTCTCTGTTTTCCTCTGTTTCTTTGTTCTGTTTGCCACACTTTTGCTGAGTGGTGGTCGAGTTTCGAGCCAAACATCTGCAGTTTTCGCCTGCGATGTTGCCAAGAATCCAGCATTGGCGAATTACAGGTTCTGCAACAAGAAATTGAGTGTGGATGCAAGGGTGAAAGACCTTGTGAGAAGGTTGACATTGCAAGAGAAGGTTGGTAACCTTGTGAATTCTGCTGTTGATGTAAGCAGACTTGGGATTCCAAAATATGAATGGTGGTCTGAAGCACTTCATGGAGTCTCTTACATTGGTCCAGGGACTCGTTTTTCCAATGTTATACCTGGAGCAACCAGTTTTCCTATGCCTATTCTCATTGCAGCTTCTTTCAATGCTTCCTTGTTTCAAACCATAGGAAAGGTAAGGTTTCTCTTATCATATCATGCATGTTAATGGTGTAAATAACAAATATTGTTTTATGGCTATTTTGTGTAGGTGGTTTCGACGGAAGCAAGAGCAATGCACAACGTCGGGTTGGCTGGTTTAACATATTGGTCACCAAACATTAACATATTTAGGGATCCAAGATGGGGAAGAGGTCAAGAAACACCAGGAGAAGACCCTTTACTTGTTAGCAAATATGCAGCAGGGTACGTGAAAGGTCTACAACAAACTGATGATGGAGACTCCAACAAGCTTAAGGTTGCTGCTTGTTGCAAACACTACACAGCATATGATGTTGATGACTGGAAAGGTGTTCAGCGTTACACCTTCAATGCTGTGGTAATGTATCAATATCATTGTTTGAAATGAAAGCTAGTTAATGTGTTTTTGAGGATTGTTTTAATTGTTGTTGATTTTGTTAGGTGACACAACAAGATTTAGATGACACATACCAACCACCTTTTAAGAGCTGTGTGATTGATGGAAATGTTGCTAGTGTTATGTGTTCCTACAATCAAGTAAATGGAAAACCGACTTGTGCTGACCCTGATCTTCTTAAAGGTGTTATCCGTGGCAAATGGAAACTAAATGGGTATCTTTTCATTTTTCAAACCTCTTAATTCTTTTATCTCACTCCATTCAAATATAAACAAAATATATTATCTCATACTTTTTTGAAAACAAGCTATAGAATGCGGCAACTTTAAAACTCGAGTGAGAAAATTGTTGTTCACTGTAATTCTATCCGGGTTGAGGAATTAGTTTTTGTAGTTGCGAACAAAGAATACCCAATTTACATCCAAAAAAATTATCTTTTATATGAGAATAAAAAAGATTGTTAGAAAATAAATATATATTAATTAAATGACATTTAGAAATAATATTATTAGATAGAGTGAAATCAAATAAGATTTATTTATATTTTAGACCAGCAAACATGAGTTTATTTTGCTTATATTGAAGATTTGAGAAAAATAATGATGTTTGCCATTCTTTTCTGTTTTCGATTTTTTTACAATAAATATGAATATGTTGTTTATCTATGTGCTATGCAGATATATAGTCTCTGATTGTGACTCAGTAGATGTGTTTTTCAAAAATCAACATTACACCAAGACCCCTGAGGAAGCTGCAGCCAAGTCCATTCTAGCAGGTAAACTACATTCGAAAAGATTACACAATCACATATTCATAGGCAACAACAGTTGAATTGTAATTTTATTTAGATGATTCATTTGAAATTTAATTAGTGTAGCTTCTTTAGAATTATACAAGGCAACTATTTATTTATCATTTTTGTGTACAAATTTTGATTTTACAGGATTGGATTTGAACTGTGGAAGTTTTCTTGACCGATATACAGAAGGTGCTGTCAAGCAAGGGCTTATAGGTGAAGCATCCATTAACAATGCCGTCTACAATAATTTTGCGACATTAATGCGTCTAGGTTTCTTTGATGGCGACCCTAGCAAGCAACCTTATGGAAACCTTGGTCCAAAAGATGTCTGCACTTCAGCAAACCAGGAGCTAGCTCGTGAAGCTGCAAGGCAAGGGATTGTGTTGCTTAAAAACTGTGCAGGATCATTGCCTCTGAATGCCAAAGCTATTAAATCATTGGCAGTTATTGGGCCCAATGCTAATGCTACTAGGGCCATGATTGGAAATTACGAAGGTATCAAATTTTCCCACTCCTATAACTTTTATAGGCAAAATGTATGATATAGTTTGTTTTTGAAGTTGAATTGACATAATATTTAAGAAATACTAACAAGTTCTAAAACTTTGCAGGCATTCCATGCAAATATACATCACCTTTGCAAGGCCTAACAGCCTTAGTTCCCACAAGCTTTGCTGCTGGCTGTCCTGATGTTCAATGCACCAATGCTGCACTAGACGATGCCAAAAAGATTGCAGCCTCAGCAGATGCAACCATGATTGTAGTCGGTGCAAATCTTGCAATAGAGGCAGAGAGTCATGACAGGATTAACATACTTCTTCCAGGACAGCAGCAACAGTTAGTTACAGAAGTTGCAAATGTAGCTAAGGGACATGTGATTCTTGCCATAATGTCTGGAGGAGGCATGGATGTTTCCTTTGCTAAAACTAATAAAAAGATCACAAGCATTCTGTGGGTTGGCTATCCTGGAGAAGCTGGTGGAGCTGCCATAGCTGATGTCATCTTTGGGTATCATAATCCAAGTAAGTCAATCTCACTGACATTCTTTCCTCATATTACAGAATTGTCATTCCAATAACTTGGATTTCCTTTTGTCACAATTTTCAAGCACTCACGCATGCAGTAAGACGAAGTTGTTGGATCAAACAACTTTGATTTTAAACTCGGTATATCAGCTTCAAAAACAAACTTGAAATTATAGTCGTTCAATCTTGATCTAATGGCTCCAAGTTGCTACAAACTTGAAATTGTAGCCGTTCGATCTTGATCTAATGGCTTCAGGTTGCTAAATGCATGAATGTGTGGAAATTGTGACGGCTTACAACCTAAATAATGGTTTCGCTCATGAAAATTGCCCCCCACAAGGTTCCAATAATTCTCAATAATATATGTTTATATATTAGCTGCATTAGCATATACACTTGTATATATTATATGCTCAGTGACCTCACTAGGATCTAATTATATGGGTATGGTCTGCACAACTTAATTTTTTTTTATTCAGGTGGAAGGCTACCCATGACATGGTATCCACAATCATATGTAGACAAAGTACCAATGACCAATATGAACATGAGGCCGGATCCTGCTACAGGCTACCCTGGTAGAACATACAGATTTTATAAAGGGGAAACTGTCTTCTCATTTGGAGATGGAATAAGCTACTCAACTTTCGAGCATAAATTGGTTAAAGCACCACAATTGGTGTCTGTTCCTTTAGCTGAGGATCACGTTTGTCGTTCCTCAAAATGTAAGTCGCTTGATGTTGTTGGCGAACATTGTCAAAACTTAGCCTTTGATATTCACCTCAGGATCAAGAACAAAGGGAAAATGAGCAGTAGCCAAACAGTGTTTTTGTTCTCTACTCCTCCTGCAGTGCACAATGCACCTCAGAAACACTTGTTGGCTTTCGAGAAAGTACACTTGACAGGAAAATCAGAAGCACTGGTCAGTTTCAAAGTAGATGTTTGTAATGATTTGAGTGTGGTTGATGAACTTGGCAACAGAAAAGTTGCCCTAGGAAAACATATGCTTCATGTAGGAGACTTGAAGCATCCTTTGAGCGTGATGATTTAA
Protein sequence

>MS.gene028420.t1

MASVENRTPNVSVFLCFFVLFATLLLSGGRVSSQTSAVFACDVAKNPALANYRFCNKKLSVDARVKDLVRRLTLQEKVGNLVNSAVDVSRLGIPKYEWWSEALHGVSYIGPGTRFSNVIPGATSFPMPILIAASFNASLFQTIGKVVSTEARAMHNVGLAGLTYWSPNINIFRDPRWGRGQETPGEDPLLVSKYAAGYVKGLQQTDDGDSNKLKVAACCKHYTAYDVDDWKGVQRYTFNAVVTQQDLDDTYQPPFKSCVIDGNVASVMCSYNQVNGKPTCADPDLLKGVIRGKWKLNGYIVSDCDSVDVFFKNQHYTKTPEEAAAKSILAGLDLNCGSFLDRYTEGAVKQGLIGEASINNAVYNNFATLMRLGFFDGDPSKQPYGNLGPKDVCTSANQELAREAARQGIVLLKNCAGSLPLNAKAIKSLAVIGPNANATRAMIGNYEGIPCKYTSPLQGLTALVPTSFAAGCPDVQCTNAALDDAKKIAASADATMIVVGANLAIEAESHDRINILLPGQQQQLVTEVANVAKGHVILAIMSGGGMDVSFAKTNKKITSILWVGYPGEAGGAAIADVIFGYHNPSGRLPMTWYPQSYVDKVPMTNMNMRPDPATGYPGRTYRFYKGETVFSFGDGISYSTFEHKLVKAPQLVSVPLAEDHVCRSSKCKSLDVVGEHCQNLAFDIHLRIKNKGKMSSSQTVFLFSTPPAVHNAPQKHLLAFEKVHLTGKSEALVSFKVDVCNDLSVVDELGNRKVALGKHMLHVGDLKHPLSVMI