Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88087.t1 | XP_003622081.1 | 98.7 | 158 | 2 | 0 | 1 | 158 | 16 | 173 | 1.20E-84 | 322.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88087.t1 | B7FIQ2 | 98.7 | 158 | 2 | 0 | 1 | 158 | 16 | 173 | 8.5e-85 | 322.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene88087 | 0.809121 | 2.21E-50 | -1.69E-46 |
| MS.gene049382 | MS.gene88087 | 0.820408 | 6.97E-53 | -1.69E-46 |
| MS.gene049758 | MS.gene88087 | 0.815241 | 1.02E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene88087 | 0.807531 | 4.83E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene88087 | 0.817857 | 2.65E-52 | -1.69E-46 |
| MS.gene050303 | MS.gene88087 | 0.80318 | 3.94E-49 | -1.69E-46 |
| MS.gene050330 | MS.gene88087 | -0.807923 | 3.99E-50 | -1.69E-46 |
| MS.gene050586 | MS.gene88087 | 0.814386 | 1.58E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene88087 | 0.830846 | 2.33E-55 | -1.69E-46 |
| MS.gene050815 | MS.gene88087 | 0.806146 | 9.48E-50 | -1.69E-46 |
| MS.gene051063 | MS.gene88087 | 0.844274 | 8.37E-59 | -1.69E-46 |
| MS.gene051245 | MS.gene88087 | 0.805849 | 1.09E-49 | -1.69E-46 |
| MS.gene051662 | MS.gene88087 | 0.826672 | 2.39E-54 | -1.69E-46 |
| MS.gene051663 | MS.gene88087 | 0.810116 | 1.35E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene88087 | 0.846771 | 1.76E-59 | -1.69E-46 |
| MS.gene052133 | MS.gene88087 | 0.808013 | 3.82E-50 | -1.69E-46 |
| MS.gene052254 | MS.gene88087 | 0.808748 | 2.66E-50 | -1.69E-46 |
| MS.gene052256 | MS.gene88087 | 0.841035 | 6.06E-58 | -1.69E-46 |
| MS.gene052286 | MS.gene88087 | 0.806718 | 7.18E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene88087 | 0.844077 | 9.45E-59 | -1.69E-46 |
| MS.gene052376 | MS.gene88087 | 0.8431 | 1.72E-58 | -1.69E-46 |
| MS.gene052665 | MS.gene88087 | 0.838055 | 3.61E-57 | -1.69E-46 |
| MS.gene052856 | MS.gene88087 | 0.820881 | 5.43E-53 | -1.69E-46 |
| MS.gene053132 | MS.gene88087 | 0.829586 | 4.74E-55 | -1.69E-46 |
| MS.gene053437 | MS.gene88087 | 0.857195 | 1.93E-62 | -1.69E-46 |
| MS.gene053630 | MS.gene88087 | 0.807447 | 5.03E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene88087 | 0.802378 | 5.77E-49 | -1.69E-46 |
| MS.gene054667 | MS.gene88087 | 0.811173 | 7.99E-51 | -1.69E-46 |
| MS.gene054942 | MS.gene88087 | 0.81938 | 1.20E-52 | -1.69E-46 |
| MS.gene055174 | MS.gene88087 | 0.835583 | 1.54E-56 | -1.69E-46 |
| MS.gene055591 | MS.gene88087 | -0.827561 | 1.46E-54 | -1.69E-46 |
| MS.gene056069 | MS.gene88087 | -0.863701 | 2.06E-64 | -1.69E-46 |
| MS.gene056432 | MS.gene88087 | 0.840924 | 6.49E-58 | -1.69E-46 |
| MS.gene056631 | MS.gene88087 | 0.801272 | 9.74E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene88087 | 0.82057 | 6.40E-53 | -1.69E-46 |
| MS.gene057057 | MS.gene88087 | 0.835991 | 1.22E-56 | -1.69E-46 |
| MS.gene057260 | MS.gene88087 | 0.828295 | 9.73E-55 | -1.69E-46 |
| MS.gene058168 | MS.gene88087 | 0.814319 | 1.64E-51 | -1.69E-46 |
| MS.gene058182 | MS.gene88087 | 0.867508 | 1.30E-65 | -1.69E-46 |
| MS.gene058851 | MS.gene88087 | 0.824317 | 8.62E-54 | -1.69E-46 |
| MS.gene058906 | MS.gene88087 | 0.860514 | 1.96E-63 | -1.69E-46 |
| MS.gene058982 | MS.gene88087 | 0.835009 | 2.15E-56 | -1.69E-46 |
| MS.gene06001 | MS.gene88087 | 0.847591 | 1.05E-59 | -1.69E-46 |
| MS.gene060165 | MS.gene88087 | 0.828811 | 7.31E-55 | -1.69E-46 |
| MS.gene060404 | MS.gene88087 | 0.814448 | 1.53E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene88087 | 0.837504 | 5.00E-57 | -1.69E-46 |
| MS.gene060619 | MS.gene88087 | 0.850258 | 1.91E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88087.t1 | MTR_7g027020 | 98.734 | 158 | 2 | 0 | 1 | 158 | 16 | 173 | 1.78e-115 | 324 |
| MS.gene88087.t1 | MTR_7g405770 | 48.322 | 149 | 68 | 5 | 18 | 158 | 26 | 173 | 8.83e-43 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88087.t1 | AT3G26960 | 51.701 | 147 | 64 | 3 | 14 | 157 | 30 | 172 | 7.79e-54 | 168 |
| MS.gene88087.t1 | AT5G41050 | 51.748 | 143 | 66 | 1 | 18 | 157 | 29 | 171 | 1.64e-53 | 167 |
| MS.gene88087.t1 | AT5G13140 | 48.611 | 144 | 71 | 1 | 18 | 158 | 35 | 178 | 1.17e-48 | 158 |
Find 23 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGTTTCCCTAGCACTTT+TGG | 0.216215 | 7.1:+69835028 | None:intergenic |
| TCTTGCCAAGCAAATTTAAT+AGG | 0.232515 | 7.1:-69834052 | MS.gene88087:CDS |
| AACACACTGATTTGAGAATT+AGG | 0.305662 | 7.1:+69835001 | None:intergenic |
| ACCACTGCTTGCAATGTTCC+TGG | 0.329830 | 7.1:-69834022 | MS.gene88087:CDS |
| CTTGCCAAGCAAATTTAATA+GGG | 0.333086 | 7.1:-69834051 | MS.gene88087:CDS |
| AACAGAACAACAAATAAGTA+TGG | 0.359972 | 7.1:-69834142 | MS.gene88087:CDS |
| ATTTGAGAATTAGGAAATCT+TGG | 0.365267 | 7.1:+69835010 | None:intergenic |
| AACTCCCTATTAAATTTGCT+TGG | 0.406122 | 7.1:+69834047 | None:intergenic |
| AATTCTCAAATCAGTGTGTT+GGG | 0.418120 | 7.1:-69834998 | MS.gene88087:CDS |
| TAATTCTCAAATCAGTGTGT+TGG | 0.461034 | 7.1:-69834999 | MS.gene88087:CDS |
| ATTGAAGTACCATCAGTTGA+TGG | 0.490136 | 7.1:-69834109 | MS.gene88087:CDS |
| AGACATAGCTACTTCTTGCC+AGG | 0.496803 | 7.1:-69834935 | MS.gene88087:intron |
| AGACCAGTGTCCTTCTTAGA+TGG | 0.511274 | 7.1:+69833914 | None:intergenic |
| CCTGCAAGACCAAAAGTGCT+AGG | 0.546586 | 7.1:-69835037 | MS.gene88087:CDS |
| CAAATAAGTATGGAATGTAC+AGG | 0.554772 | 7.1:-69834132 | MS.gene88087:CDS |
| ATAATTATGTTCAGGTGCTG+AGG | 0.580549 | 7.1:-69834230 | MS.gene88087:intron |
| CATCTCACTCCATCAACTGA+TGG | 0.589191 | 7.1:+69834100 | None:intergenic |
| AAGAAGGACACTGGTCTATG+TGG | 0.605551 | 7.1:-69833908 | MS.gene88087:CDS |
| TCCAGGAACATTGCAAGCAG+TGG | 0.607145 | 7.1:+69834021 | None:intergenic |
| CTGCAAGACCAAAAGTGCTA+GGG | 0.608365 | 7.1:-69835036 | MS.gene88087:CDS |
| ATGTGATTTCAGTCAAAGCA+AGG | 0.609000 | 7.1:-69833982 | MS.gene88087:CDS |
| AGACCATCTAAGAAGGACAC+TGG | 0.644130 | 7.1:-69833917 | MS.gene88087:CDS |
| GATGGAGTGAGATGTGCTGA+AGG | 0.665515 | 7.1:-69834091 | MS.gene88087:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAATTTTAAAAAAAATAT+TGG | - | chr7.1:69834121-69834140 | MS.gene88087:CDS | 0.0% |
| !!! | TTTTAAAAAATATATATATT+TGG | - | chr7.1:69834374-69834393 | MS.gene88087:intron | 0.0% |
| !! | AGAAATTACTATAAATTAAA+AGG | - | chr7.1:69834147-69834166 | MS.gene88087:CDS | 10.0% |
| !! | ATTAAAAACATTCATTAATG+TGG | - | chr7.1:69834268-69834287 | MS.gene88087:intron | 15.0% |
| !! | GGTGAAAAAATAAATATATT+TGG | - | chr7.1:69834395-69834414 | MS.gene88087:intron | 15.0% |
| !! | TTACTATAAATTAAAAGGAT+AGG | - | chr7.1:69834152-69834171 | MS.gene88087:CDS | 15.0% |
| !!! | ATGAATGTTTTTAATTTATG+TGG | + | chr7.1:69834263-69834282 | None:intergenic | 15.0% |
| !! | GGATTTAAAAAAGAGATTAA+TGG | - | chr7.1:69834177-69834196 | MS.gene88087:CDS | 20.0% |
| !! | TATAAATTAAAAGGATAGGA+AGG | - | chr7.1:69834156-69834175 | MS.gene88087:CDS | 20.0% |
| !! | TTTAAAAAAGAGATTAATGG+TGG | - | chr7.1:69834180-69834199 | MS.gene88087:CDS | 20.0% |
| !! | TTTCTTTCATAATTATGTTC+AGG | - | chr7.1:69834715-69834734 | MS.gene88087:intron | 20.0% |
| !!! | CATTTTACAACTTTTTATCA+AGG | - | chr7.1:69834460-69834479 | MS.gene88087:intron | 20.0% |
| ! | AACAGAACAACAAATAAGTA+TGG | - | chr7.1:69834811-69834830 | MS.gene88087:intron | 25.0% |
| ! | ACACTCTATTTGAACTATTT+CGG | + | chr7.1:69834102-69834121 | None:intergenic | 25.0% |
| ! | ATTTGAGAATTAGGAAATCT+TGG | + | chr7.1:69833946-69833965 | None:intergenic | 25.0% |
| ! | GGTTATTCAAAATTATTACC+TGG | + | chr7.1:69834039-69834058 | None:intergenic | 25.0% |
| !! | AAATTTTAGACCATCTAAGA+AGG | - | chr7.1:69835029-69835048 | MS.gene88087:CDS | 25.0% |
| !! | ACTAATTTTTGTTAACTCAG+TGG | + | chr7.1:69834676-69834695 | None:intergenic | 25.0% |
| !! | TAGTTTAACTTTCGTAGAAA+TGG | + | chr7.1:69834650-69834669 | None:intergenic | 25.0% |
| !! | TTATATGTGCAAAGATGTTT+TGG | - | chr7.1:69834313-69834332 | MS.gene88087:intron | 25.0% |
| !! | TTGTGTATGTAAAGTTGTTT+TGG | + | chr7.1:69834218-69834237 | None:intergenic | 25.0% |
| !! | TTTTTCCTGAAGACAAAATT+GGG | - | chr7.1:69834578-69834597 | MS.gene88087:intron | 25.0% |
| !!! | TTTTTTCCTGAAGACAAAAT+TGG | - | chr7.1:69834577-69834596 | MS.gene88087:intron | 25.0% |
| AAAAAGAGATTAATGGTGGT+AGG | - | chr7.1:69834184-69834203 | MS.gene88087:CDS | 30.0% | |
| AATTCTCAAATCAGTGTGTT+GGG | - | chr7.1:69833955-69833974 | MS.gene88087:CDS | 30.0% | |
| ATGCAAACAATTAACACTAG+AGG | + | chr7.1:69834060-69834079 | None:intergenic | 30.0% | |
| CAAATAAGTATGGAATGTAC+AGG | - | chr7.1:69834821-69834840 | MS.gene88087:intron | 30.0% | |
| CTTGCCAAGCAAATTTAATA+GGG | - | chr7.1:69834902-69834921 | MS.gene88087:intron | 30.0% | |
| TAATTCTCAAATCAGTGTGT+TGG | - | chr7.1:69833954-69833973 | MS.gene88087:CDS | 30.0% | |
| TCTTGCCAAGCAAATTTAAT+AGG | - | chr7.1:69834901-69834920 | MS.gene88087:intron | 30.0% | |
| ! | AACTCCCTATTAAATTTGCT+TGG | + | chr7.1:69834909-69834928 | None:intergenic | 30.0% |
| ! | TTTTCCTGAAGACAAAATTG+GGG | - | chr7.1:69834579-69834598 | MS.gene88087:intron | 30.0% |
| !! | AACACACTGATTTGAGAATT+AGG | + | chr7.1:69833955-69833974 | None:intergenic | 30.0% |
| !!! | GCAATTCTAAGTGTTTTGAT+TGG | - | chr7.1:69834290-69834309 | MS.gene88087:intron | 30.0% |
| !!! | GTATGTAAAGTTGTTTTGGA+CGG | + | chr7.1:69834214-69834233 | None:intergenic | 30.0% |
| ATGTGATTTCAGTCAAAGCA+AGG | - | chr7.1:69834971-69834990 | MS.gene88087:CDS | 35.0% | |
| ! | ATAATTATGTTCAGGTGCTG+AGG | - | chr7.1:69834723-69834742 | MS.gene88087:intron | 35.0% |
| ! | ATTGAAGTACCATCAGTTGA+TGG | - | chr7.1:69834844-69834863 | MS.gene88087:intron | 35.0% |
| ! | TATACCCCAATTTTGTCTTC+AGG | + | chr7.1:69834586-69834605 | None:intergenic | 35.0% |
| ! | CAGTCAAGTTGTTATCACTG+AGG | - | chr7.1:69834337-69834356 | MS.gene88087:intron | 40.0% |
| ! | TTAGCAGTGCTTTTGTATCC+AGG | + | chr7.1:69834952-69834971 | None:intergenic | 40.0% |
| AGACATAGCTACTTCTTGCC+AGG | - | chr7.1:69834018-69834037 | MS.gene88087:CDS | 45.0% | |
| AGACCAGTGTCCTTCTTAGA+TGG | + | chr7.1:69835042-69835061 | None:intergenic | 45.0% | |
| AGACCATCTAAGAAGGACAC+TGG | - | chr7.1:69835036-69835055 | MS.gene88087:CDS | 45.0% | |
| CATCTCACTCCATCAACTGA+TGG | + | chr7.1:69834856-69834875 | None:intergenic | 45.0% | |
| ! | AAGAAGGACACTGGTCTATG+TGG | - | chr7.1:69835045-69835064 | MS.gene88087:CDS | 45.0% |
| ! | CTTGGTTTCCCTAGCACTTT+TGG | + | chr7.1:69833928-69833947 | None:intergenic | 45.0% |
| !! | CTGCAAGACCAAAAGTGCTA+GGG | - | chr7.1:69833917-69833936 | MS.gene88087:CDS | 45.0% |
| !!! | TATATTTTTTAAAATATATG+AGG | + | chr7.1:69834368-69834387 | None:intergenic | 5.0% |
| ACCACTGCTTGCAATGTTCC+TGG | - | chr7.1:69834931-69834950 | MS.gene88087:intron | 50.0% | |
| GATGGAGTGAGATGTGCTGA+AGG | - | chr7.1:69834862-69834881 | MS.gene88087:intron | 50.0% | |
| TCCAGGAACATTGCAAGCAG+TGG | + | chr7.1:69834935-69834954 | None:intergenic | 50.0% | |
| !! | CCTGCAAGACCAAAAGTGCT+AGG | - | chr7.1:69833916-69833935 | MS.gene88087:CDS | 50.0% |
| !!! | CCTAGCACTTTTGGTCTTGC+AGG | + | chr7.1:69833919-69833938 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 69833901 | 69835074 | 69833901 | ID=MS.gene88087 |
| chr7.1 | mRNA | 69833901 | 69835074 | 69833901 | ID=MS.gene88087.t1;Parent=MS.gene88087 |
| chr7.1 | exon | 69834936 | 69835074 | 69834936 | ID=MS.gene88087.t1.exon1;Parent=MS.gene88087.t1 |
| chr7.1 | CDS | 69834936 | 69835074 | 69834936 | ID=cds.MS.gene88087.t1;Parent=MS.gene88087.t1 |
| chr7.1 | exon | 69833901 | 69834238 | 69833901 | ID=MS.gene88087.t1.exon2;Parent=MS.gene88087.t1 |
| chr7.1 | CDS | 69833901 | 69834238 | 69833901 | ID=cds.MS.gene88087.t1;Parent=MS.gene88087.t1 |
| Gene Sequence |
| Protein sequence |
