Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91970.t1 | XP_003618009.2 | 95.1 | 164 | 7 | 1 | 1 | 163 | 1 | 164 | 1.30E-80 | 309.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91970.t1 | G7K4Z5 | 95.1 | 164 | 7 | 1 | 1 | 163 | 1 | 164 | 9.4e-81 | 309.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene91970 | 0.855444 | 6.28E-62 | -1.69E-46 |
| MS.gene049369 | MS.gene91970 | 0.835603 | 1.52E-56 | -1.69E-46 |
| MS.gene049661 | MS.gene91970 | 0.854161 | 1.48E-61 | -1.69E-46 |
| MS.gene049662 | MS.gene91970 | 0.847208 | 1.34E-59 | -1.69E-46 |
| MS.gene049758 | MS.gene91970 | 0.835327 | 1.79E-56 | -1.69E-46 |
| MS.gene050128 | MS.gene91970 | 0.827529 | 1.49E-54 | -1.69E-46 |
| MS.gene050301 | MS.gene91970 | 0.837757 | 4.31E-57 | -1.69E-46 |
| MS.gene050397 | MS.gene91970 | 0.804005 | 2.66E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene91970 | 0.825026 | 5.87E-54 | -1.69E-46 |
| MS.gene050670 | MS.gene91970 | 0.808981 | 2.37E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene91970 | 0.813372 | 2.65E-51 | -1.69E-46 |
| MS.gene052078 | MS.gene91970 | 0.809608 | 1.74E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene91970 | 0.84399 | 9.97E-59 | -1.69E-46 |
| MS.gene052253 | MS.gene91970 | 0.828592 | 8.25E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene91970 | 0.827392 | 1.60E-54 | -1.69E-46 |
| MS.gene052340 | MS.gene91970 | 0.831346 | 1.76E-55 | -1.69E-46 |
| MS.gene052485 | MS.gene91970 | 0.817299 | 3.54E-52 | -1.69E-46 |
| MS.gene053073 | MS.gene91970 | 0.800494 | 1.40E-48 | -1.69E-46 |
| MS.gene05316 | MS.gene91970 | 0.818403 | 2.00E-52 | -1.69E-46 |
| MS.gene053209 | MS.gene91970 | 0.820831 | 5.57E-53 | -1.69E-46 |
| MS.gene053836 | MS.gene91970 | 0.828598 | 8.22E-55 | -1.69E-46 |
| MS.gene053928 | MS.gene91970 | 0.80002 | 1.75E-48 | -1.69E-46 |
| MS.gene054123 | MS.gene91970 | 0.833172 | 6.21E-56 | -1.69E-46 |
| MS.gene054349 | MS.gene91970 | 0.806809 | 6.87E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene91970 | 0.863988 | 1.68E-64 | -1.69E-46 |
| MS.gene055277 | MS.gene91970 | 0.818536 | 1.86E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene91970 | 0.829026 | 6.48E-55 | -1.69E-46 |
| MS.gene055428 | MS.gene91970 | 0.802034 | 6.79E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene91970 | 0.812398 | 4.33E-51 | -1.69E-46 |
| MS.gene056191 | MS.gene91970 | 0.816686 | 4.87E-52 | -1.69E-46 |
| MS.gene056407 | MS.gene91970 | 0.827218 | 1.77E-54 | -1.69E-46 |
| MS.gene056410 | MS.gene91970 | 0.804367 | 2.23E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene91970 | 0.83556 | 1.56E-56 | -1.69E-46 |
| MS.gene056609 | MS.gene91970 | 0.814058 | 1.87E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene91970 | 0.835873 | 1.30E-56 | -1.69E-46 |
| MS.gene05735 | MS.gene91970 | 0.813892 | 2.03E-51 | -1.69E-46 |
| MS.gene057448 | MS.gene91970 | 0.840649 | 7.66E-58 | -1.69E-46 |
| MS.gene057449 | MS.gene91970 | 0.842124 | 3.13E-58 | -1.69E-46 |
| MS.gene057450 | MS.gene91970 | 0.848677 | 5.26E-60 | -1.69E-46 |
| MS.gene057749 | MS.gene91970 | 0.809505 | 1.83E-50 | -1.69E-46 |
| MS.gene057823 | MS.gene91970 | 0.811035 | 8.56E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene91970 | 0.813599 | 2.36E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene91970 | 0.820805 | 5.65E-53 | -1.69E-46 |
| MS.gene058787 | MS.gene91970 | 0.813998 | 1.93E-51 | -1.69E-46 |
| MS.gene058928 | MS.gene91970 | 0.850132 | 2.07E-60 | -1.69E-46 |
| MS.gene058949 | MS.gene91970 | 0.819117 | 1.37E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene91970 | 0.822263 | 2.60E-53 | -1.69E-46 |
| MS.gene059285 | MS.gene91970 | 0.817588 | 3.05E-52 | -1.69E-46 |
| MS.gene059366 | MS.gene91970 | 0.814698 | 1.35E-51 | -1.69E-46 |
| MS.gene059597 | MS.gene91970 | 0.843531 | 1.32E-58 | -1.69E-46 |
| MS.gene059674 | MS.gene91970 | 0.81074 | 9.92E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene91970 | 0.81833 | 2.07E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene91970 | 0.845323 | 4.36E-59 | -1.69E-46 |
| MS.gene059692 | MS.gene91970 | 0.844145 | 9.06E-59 | -1.69E-46 |
| MS.gene059695 | MS.gene91970 | 0.841958 | 3.46E-58 | -1.69E-46 |
| MS.gene059711 | MS.gene91970 | 0.808218 | 3.45E-50 | -1.69E-46 |
| MS.gene059807 | MS.gene91970 | 0.852142 | 5.61E-61 | -1.69E-46 |
| MS.gene059876 | MS.gene91970 | 0.807758 | 4.32E-50 | -1.69E-46 |
| MS.gene059894 | MS.gene91970 | 0.819823 | 9.49E-53 | -1.69E-46 |
| MS.gene06037 | MS.gene91970 | 0.873964 | 9.78E-68 | -1.69E-46 |
| MS.gene060403 | MS.gene91970 | 0.824231 | 9.03E-54 | -1.69E-46 |
| MS.gene060495 | MS.gene91970 | 0.856299 | 3.53E-62 | -1.69E-46 |
| MS.gene060837 | MS.gene91970 | 0.802489 | 5.48E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene91970 | 0.82081 | 5.63E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene91970 | 0.861885 | 7.49E-64 | -1.69E-46 |
| MS.gene061051 | MS.gene91970 | -0.847227 | 1.32E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91970.t1 | MTR_5g097970 | 95.122 | 164 | 7 | 1 | 1 | 163 | 1 | 164 | 5.37e-112 | 317 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91970.t1 | AT2G04790 | 62.069 | 145 | 52 | 2 | 15 | 156 | 36 | 180 | 1.05e-63 | 195 |
| MS.gene91970.t1 | AT2G04790 | 62.069 | 145 | 52 | 2 | 15 | 156 | 34 | 178 | 1.18e-63 | 195 |
| MS.gene91970.t1 | AT2G04790 | 62.069 | 145 | 52 | 2 | 15 | 156 | 17 | 161 | 2.22e-63 | 194 |
| MS.gene91970.t1 | AT2G04790 | 64.662 | 133 | 44 | 2 | 15 | 144 | 36 | 168 | 4.77e-61 | 188 |
| MS.gene91970.t1 | AT2G04790 | 64.662 | 133 | 44 | 2 | 15 | 144 | 34 | 166 | 5.27e-61 | 188 |
| MS.gene91970.t1 | AT2G04790 | 64.662 | 133 | 44 | 2 | 15 | 144 | 17 | 149 | 8.61e-61 | 187 |
| MS.gene91970.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 36 | 112 | 2.10e-31 | 111 |
| MS.gene91970.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 36 | 112 | 2.10e-31 | 111 |
| MS.gene91970.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 17 | 93 | 4.43e-31 | 109 |
| MS.gene91970.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 17 | 93 | 4.43e-31 | 109 |
| MS.gene91970.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 36 | 112 | 4.87e-31 | 110 |
| MS.gene91970.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 36 | 112 | 4.87e-31 | 110 |
| MS.gene91970.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 17 | 93 | 9.37e-31 | 109 |
| MS.gene91970.t1 | AT2G04790 | 72.727 | 44 | 12 | 0 | 15 | 58 | 36 | 79 | 7.97e-18 | 75.1 |
| MS.gene91970.t1 | AT2G04790 | 72.727 | 44 | 12 | 0 | 15 | 58 | 36 | 79 | 7.97e-18 | 75.1 |
| MS.gene91970.t1 | AT2G04790 | 72.727 | 44 | 12 | 0 | 15 | 58 | 36 | 79 | 7.97e-18 | 75.1 |
Find 43 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCTTACTCCCTTAATATT+TGG | 0.106470 | 5.2:-82505227 | MS.gene91970:CDS |
| ATCACTTTCCTCGATTTATT+TGG | 0.194419 | 5.2:+82495488 | None:intergenic |
| GCTGCAAATCCCAAATATTA+AGG | 0.224867 | 5.2:+82505217 | None:intergenic |
| TGAAAATGAGAGATGGAATT+TGG | 0.232618 | 5.2:-82495687 | MS.gene91970:CDS |
| TGCTTACTCCCTTAATATTT+GGG | 0.251158 | 5.2:-82505226 | MS.gene91970:CDS |
| TATATATCTTCAGGATCTTT+TGG | 0.303960 | 5.2:+82504964 | None:intergenic |
| ATGTGGCAGCAATGCCTCTA+AGG | 0.323320 | 5.2:-82505284 | MS.gene91970:CDS |
| CCTCGATTTATTTGGCTTTG+AGG | 0.364586 | 5.2:+82495496 | None:intergenic |
| TTGGGATTTGCAGCATTTCA+TGG | 0.365079 | 5.2:-82505208 | MS.gene91970:CDS |
| GGTCCCTTTGAAGCCCTTAG+AGG | 0.394810 | 5.2:+82505270 | None:intergenic |
| CAAACCTTCCTCACCTTCAA+AGG | 0.430343 | 5.2:-82505178 | MS.gene91970:intron |
| CTGCAAATCCCAAATATTAA+GGG | 0.434798 | 5.2:+82505218 | None:intergenic |
| ACCAACCAGCATTTGCTTCG+CGG | 0.449071 | 5.2:+82504761 | None:intergenic |
| AATAGGATTGAAACCTTTGA+AGG | 0.455552 | 5.2:+82505165 | None:intergenic |
| GGTTTGGTCATGCAACTCAT+TGG | 0.462182 | 5.2:-82495864 | MS.gene91970:CDS |
| AATGCCTCTAAGGGCTTCAA+AGG | 0.472734 | 5.2:-82505274 | MS.gene91970:CDS |
| AGTGCCGCATATATATCTTC+AGG | 0.473960 | 5.2:+82504955 | None:intergenic |
| TGGGAAACAGATTTGAGAAT+CGG | 0.477510 | 5.2:-82504686 | MS.gene91970:CDS |
| AGAAGAAGATGCTGTGGAAA+TGG | 0.484961 | 5.2:-82504729 | MS.gene91970:CDS |
| CGAAGCAAATGCTGGTTGGT+TGG | 0.522860 | 5.2:-82504758 | MS.gene91970:CDS |
| GAAACCTTTGAAGGTGAGGA+AGG | 0.532186 | 5.2:+82505174 | None:intergenic |
| AGGCAGAGAGGAAGTGTATG+TGG | 0.544412 | 5.2:-82505301 | MS.gene91970:CDS |
| TTATCTGGTAGAGCTGTGCC+AGG | 0.545939 | 5.2:-82504925 | MS.gene91970:intron |
| TCAAAGACAAGAATGGTAAG+AGG | 0.550312 | 5.2:-82505321 | MS.gene91970:CDS |
| TGAATTACCTCTCGCTACCC+TGG | 0.553210 | 5.2:+82495811 | None:intergenic |
| GATTGCCGCGAAGCAAATGC+TGG | 0.556329 | 5.2:-82504766 | MS.gene91970:CDS |
| AGTTGTATCAAAGACAAGAA+TGG | 0.561294 | 5.2:-82505328 | MS.gene91970:CDS |
| TGTGGCAGCAATGCCTCTAA+GGG | 0.570128 | 5.2:-82505283 | MS.gene91970:CDS |
| GATTGTCGCGATTATACCAA+TGG | 0.578050 | 5.2:-82504656 | MS.gene91970:intron |
| CTCAAAGTGAAAATGAGAGA+TGG | 0.582340 | 5.2:-82495694 | MS.gene91970:CDS |
| ATGCCTCTAAGGGCTTCAAA+GGG | 0.584284 | 5.2:-82505273 | MS.gene91970:CDS |
| GATTGAAACCTTTGAAGGTG+AGG | 0.593749 | 5.2:+82505170 | None:intergenic |
| GCTGAAACGTTTGAGAAACC+AGG | 0.597971 | 5.2:-82495829 | MS.gene91970:CDS |
| GCAGCAGACATAAGCAATTG+TGG | 0.599579 | 5.2:+82505246 | None:intergenic |
| TCTCAGGAGCTGTTCTTGTG+AGG | 0.603725 | 5.2:-82504799 | MS.gene91970:intron |
| GCCGCGAAGCAAATGCTGGT+TGG | 0.618375 | 5.2:-82504762 | MS.gene91970:CDS |
| AAGAATGGTAAGAGGCAGAG+AGG | 0.650232 | 5.2:-82505313 | MS.gene91970:CDS |
| CTGAAACGTTTGAGAAACCA+GGG | 0.653890 | 5.2:-82495828 | MS.gene91970:CDS |
| CCTCAAAGCCAAATAAATCG+AGG | 0.665555 | 5.2:-82495496 | MS.gene91970:CDS |
| AGATCCTGAAGATATATATG+CGG | 0.681441 | 5.2:-82504959 | MS.gene91970:CDS |
| ATATGCAGAAGAAGATGCTG+TGG | 0.682714 | 5.2:-82504735 | MS.gene91970:CDS |
| TGAGAAACCAGGGTAGCGAG+AGG | 0.685947 | 5.2:-82495818 | MS.gene91970:intron |
| GCAGACATAAGCAATTGTGG+TGG | 0.708742 | 5.2:+82505249 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 82495489 | 82505358 | 82495489 | ID=MS.gene91970 |
| chr5.2 | mRNA | 82495489 | 82505358 | 82495489 | ID=MS.gene91970.t1;Parent=MS.gene91970 |
| chr5.2 | exon | 82505179 | 82505358 | 82505179 | ID=MS.gene91970.t1.exon1;Parent=MS.gene91970.t1 |
| chr5.2 | CDS | 82505179 | 82505358 | 82505179 | ID=cds.MS.gene91970.t1;Parent=MS.gene91970.t1 |
| chr5.2 | exon | 82504926 | 82505007 | 82504926 | ID=MS.gene91970.t1.exon2;Parent=MS.gene91970.t1 |
| chr5.2 | CDS | 82504926 | 82505007 | 82504926 | ID=cds.MS.gene91970.t1;Parent=MS.gene91970.t1 |
| chr5.2 | exon | 82504657 | 82504815 | 82504657 | ID=MS.gene91970.t1.exon3;Parent=MS.gene91970.t1 |
| chr5.2 | CDS | 82504657 | 82504815 | 82504657 | ID=cds.MS.gene91970.t1;Parent=MS.gene91970.t1 |
| chr5.2 | exon | 82495819 | 82495885 | 82495819 | ID=MS.gene91970.t1.exon4;Parent=MS.gene91970.t1 |
| chr5.2 | CDS | 82495819 | 82495885 | 82495819 | ID=cds.MS.gene91970.t1;Parent=MS.gene91970.t1 |
| chr5.2 | exon | 82495654 | 82495718 | 82495654 | ID=MS.gene91970.t1.exon5;Parent=MS.gene91970.t1 |
| chr5.2 | CDS | 82495654 | 82495718 | 82495654 | ID=cds.MS.gene91970.t1;Parent=MS.gene91970.t1 |
| chr5.2 | exon | 82495489 | 82495538 | 82495489 | ID=MS.gene91970.t1.exon6;Parent=MS.gene91970.t1 |
| chr5.2 | CDS | 82495489 | 82495538 | 82495489 | ID=cds.MS.gene91970.t1;Parent=MS.gene91970.t1 |
| Gene Sequence |
| Protein sequence |
