Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93665.t1 | KEH23142.1 | 93.4 | 257 | 17 | 0 | 1 | 257 | 1 | 257 | 2.60E-105 | 391.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93665.t1 | A0A072U0F6 | 93.4 | 257 | 17 | 0 | 1 | 257 | 1 | 257 | 1.8e-105 | 391.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049195 | MS.gene93665 | 0.802481 | 5.50E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene93665 | 0.806592 | 7.63E-50 | -1.69E-46 |
| MS.gene049993 | MS.gene93665 | 0.823981 | 1.03E-53 | -1.69E-46 |
| MS.gene050406 | MS.gene93665 | 0.804744 | 1.86E-49 | -1.69E-46 |
| MS.gene050426 | MS.gene93665 | -0.81254 | 4.03E-51 | -1.69E-46 |
| MS.gene050464 | MS.gene93665 | 0.819211 | 1.31E-52 | -1.69E-46 |
| MS.gene050529 | MS.gene93665 | 0.800369 | 1.49E-48 | -1.69E-46 |
| MS.gene050670 | MS.gene93665 | 0.823641 | 1.24E-53 | -1.69E-46 |
| MS.gene051208 | MS.gene93665 | 0.801213 | 1.00E-48 | -1.69E-46 |
| MS.gene051525 | MS.gene93665 | 0.825085 | 5.68E-54 | -1.69E-46 |
| MS.gene051582 | MS.gene93665 | 0.881407 | 2.46E-70 | -1.69E-46 |
| MS.gene051983 | MS.gene93665 | 0.81111 | 8.25E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene93665 | -0.836532 | 8.86E-57 | -1.69E-46 |
| MS.gene052077 | MS.gene93665 | 0.828148 | 1.06E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene93665 | 0.803376 | 3.59E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene93665 | 0.808649 | 2.79E-50 | -1.69E-46 |
| MS.gene053330 | MS.gene93665 | 0.843216 | 1.61E-58 | -1.69E-46 |
| MS.gene053836 | MS.gene93665 | 0.841251 | 5.32E-58 | -1.69E-46 |
| MS.gene054102 | MS.gene93665 | 0.805236 | 1.47E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene93665 | 0.825141 | 5.51E-54 | -1.69E-46 |
| MS.gene054719 | MS.gene93665 | 0.811738 | 6.02E-51 | -1.69E-46 |
| MS.gene054918 | MS.gene93665 | 0.84353 | 1.32E-58 | -1.69E-46 |
| MS.gene054983 | MS.gene93665 | 0.834313 | 3.22E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene93665 | 0.82354 | 1.31E-53 | -1.69E-46 |
| MS.gene055428 | MS.gene93665 | 0.816972 | 4.20E-52 | -1.69E-46 |
| MS.gene05553 | MS.gene93665 | 0.860203 | 2.43E-63 | -1.69E-46 |
| MS.gene055712 | MS.gene93665 | 0.811432 | 7.02E-51 | -1.69E-46 |
| MS.gene05594 | MS.gene93665 | 0.802055 | 6.73E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene93665 | 0.830923 | 2.23E-55 | -1.69E-46 |
| MS.gene056612 | MS.gene93665 | 0.816293 | 5.96E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene93665 | 0.822184 | 2.71E-53 | -1.69E-46 |
| MS.gene056899 | MS.gene93665 | -0.827542 | 1.48E-54 | -1.69E-46 |
| MS.gene056979 | MS.gene93665 | 0.803619 | 3.20E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene93665 | 0.824977 | 6.03E-54 | -1.69E-46 |
| MS.gene058002 | MS.gene93665 | 0.819318 | 1.24E-52 | -1.69E-46 |
| MS.gene058142 | MS.gene93665 | 0.830898 | 2.27E-55 | -1.69E-46 |
| MS.gene058276 | MS.gene93665 | 0.82444 | 8.06E-54 | -1.69E-46 |
| MS.gene05886 | MS.gene93665 | 0.826775 | 2.25E-54 | -1.69E-46 |
| MS.gene059011 | MS.gene93665 | 0.816722 | 4.78E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene93665 | 0.812466 | 4.18E-51 | -1.69E-46 |
| MS.gene059141 | MS.gene93665 | 0.805783 | 1.13E-49 | -1.69E-46 |
| MS.gene059247 | MS.gene93665 | 0.828223 | 1.01E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene93665 | 0.823064 | 1.69E-53 | -1.69E-46 |
| MS.gene060403 | MS.gene93665 | 0.826648 | 2.42E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene93665 | 0.829273 | 5.65E-55 | -1.69E-46 |
| MS.gene061114 | MS.gene93665 | -0.829562 | 4.80E-55 | -1.69E-46 |
| MS.gene061159 | MS.gene93665 | 0.811118 | 8.22E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93665.t1 | MTR_7g067000 | 93.774 | 257 | 16 | 0 | 1 | 257 | 1 | 257 | 1.24e-174 | 481 |
| MS.gene93665.t1 | MTR_4g015010 | 56.349 | 252 | 97 | 5 | 1 | 239 | 14 | 265 | 4.38e-85 | 255 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93665.t1 | AT3G09050 | 50.988 | 253 | 105 | 7 | 4 | 243 | 2 | 248 | 7.69e-76 | 231 |
Find 38 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGCATACTTAACTCAATT+TGG | 0.207885 | 7.1:+34605755 | MS.gene93665:CDS |
| CAACAGGTACCTTCTTTCTA+AGG | 0.304642 | 7.1:-34606078 | None:intergenic |
| AATGTTTACCTCTTCTTCAT+TGG | 0.306206 | 7.1:+34606181 | MS.gene93665:CDS |
| GTGTTTGAAGCGTGACAATT+TGG | 0.310066 | 7.1:-34605697 | None:intergenic |
| TAAAGGCTGGTAGCGGAATC+AGG | 0.365890 | 7.1:+34606155 | MS.gene93665:CDS |
| TTGCGTCGCCTTCGACTGAT+TGG | 0.388513 | 7.1:+34606233 | MS.gene93665:CDS |
| CTTTAAGAGCCTTAGAAAGA+AGG | 0.401246 | 7.1:+34606069 | MS.gene93665:CDS |
| TGTTTGAAGCGTGACAATTT+GGG | 0.431403 | 7.1:-34605696 | None:intergenic |
| AACGCCGAGTTTGAAAAGAT+TGG | 0.433296 | 7.1:+34605788 | MS.gene93665:CDS |
| AGCGGAGTTTGAAGTTCAAA+TGG | 0.433795 | 7.1:+34606021 | MS.gene93665:CDS |
| TCCGCTACCAGCCTTTAGTC+TGG | 0.451103 | 7.1:-34606149 | None:intergenic |
| TGAGTTTGAAGGAAGGATGT+AGG | 0.478381 | 7.1:-34605541 | None:intergenic |
| ATTCCTCGAATTCTAGCATC+TGG | 0.482256 | 7.1:-34605952 | None:intergenic |
| TTTGAAGTTCAAATGGAAAA+CGG | 0.485912 | 7.1:+34606028 | MS.gene93665:CDS |
| TTGTGCTTATGTAAGTCCAA+TGG | 0.488206 | 7.1:+34605620 | MS.gene93665:CDS |
| TGTGCTTATGTAAGTCCAAT+GGG | 0.490101 | 7.1:+34605621 | MS.gene93665:CDS |
| CTTGATGTTGGTGAGTTTGA+AGG | 0.524254 | 7.1:-34605552 | None:intergenic |
| AAACTACTGTAAAGGTCAGC+AGG | 0.526715 | 7.1:-34606204 | None:intergenic |
| AGCTTGAGAATCAGACCCAT+TGG | 0.534125 | 7.1:-34605636 | None:intergenic |
| GAGTTTGAAGGAAGGATGTA+GGG | 0.534701 | 7.1:-34605540 | None:intergenic |
| GAGCCAGATGCTAGAATTCG+AGG | 0.536260 | 7.1:+34605949 | MS.gene93665:CDS |
| AATAGAATAAACTACTGTAA+AGG | 0.541708 | 7.1:-34606212 | None:intergenic |
| TAAAGATGTCACCAGACTAA+AGG | 0.542082 | 7.1:+34606138 | MS.gene93665:CDS |
| ATCTTCAGAAATTAACAGAC+AGG | 0.549990 | 7.1:+34605973 | MS.gene93665:CDS |
| GATTCTCAAGCTCCTCAACC+AGG | 0.562806 | 7.1:+34605647 | MS.gene93665:CDS |
| AGAGCGTGAAAACGAATTAG+CGG | 0.567801 | 7.1:+34606003 | MS.gene93665:CDS |
| ATGGAAAACGGCAGAAACGA+AGG | 0.568917 | 7.1:+34606040 | MS.gene93665:CDS |
| TTAACAGATAACAGCGAACA+TGG | 0.573660 | 7.1:+34605925 | MS.gene93665:intron |
| ACCAGACTAAAGGCTGGTAG+CGG | 0.574115 | 7.1:+34606148 | MS.gene93665:CDS |
| TCAGCAGGCCAATGAAGAAG+AGG | 0.579281 | 7.1:-34606189 | None:intergenic |
| AAGAAAGACCAACAATGAAG+AGG | 0.585477 | 7.1:+34606345 | MS.gene93665:CDS |
| ATGTTGGTGAGTTTGAAGGA+AGG | 0.590472 | 7.1:-34605548 | None:intergenic |
| GTGGATATTACACTTGATGT+TGG | 0.603769 | 7.1:-34605564 | None:intergenic |
| GATGTCACCAGACTAAAGGC+TGG | 0.604550 | 7.1:+34606142 | MS.gene93665:CDS |
| CATCTAGATATTCTGTAAGA+CGG | 0.624579 | 7.1:-34605727 | None:intergenic |
| TGATAAAGCAAAAGCCAAAG+AGG | 0.625476 | 7.1:+34606102 | MS.gene93665:CDS |
| TGGAAAACGGCAGAAACGAA+GGG | 0.629953 | 7.1:+34606041 | MS.gene93665:CDS |
| TAAAGCAAAAGCCAAAGAGG+AGG | 0.659588 | 7.1:+34606105 | MS.gene93665:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATACAAAATGAATATAAG+AGG | - | chr7.1:34605907-34605926 | None:intergenic | 15.0% |
| !!! | TTTTTTATTTACATTTGACA+TGG | - | chr7.1:34605881-34605900 | None:intergenic | 15.0% |
| !! | AATAGAATAAACTACTGTAA+AGG | - | chr7.1:34606215-34606234 | None:intergenic | 20.0% |
| !! | TTTGAAGTTCAAATGGAAAA+CGG | + | chr7.1:34606028-34606047 | MS.gene93665:CDS | 25.0% |
| !!! | TTTATTTTCCTCTTCATTGT+TGG | - | chr7.1:34606356-34606375 | None:intergenic | 25.0% |
| AATGTTTACCTCTTCTTCAT+TGG | + | chr7.1:34606181-34606200 | MS.gene93665:CDS | 30.0% | |
| ATCTTCAGAAATTAACAGAC+AGG | + | chr7.1:34605973-34605992 | MS.gene93665:CDS | 30.0% | |
| CATCTAGATATTCTGTAAGA+CGG | - | chr7.1:34605730-34605749 | None:intergenic | 30.0% | |
| TCTGCATACTTAACTCAATT+TGG | + | chr7.1:34605755-34605774 | MS.gene93665:CDS | 30.0% | |
| ! | TGGAGAAAAGTAACAGTTTT+TGG | + | chr7.1:34606253-34606272 | MS.gene93665:CDS | 30.0% |
| ! | TTCTCCAATCTTTTCAAACT+CGG | - | chr7.1:34605795-34605814 | None:intergenic | 30.0% |
| !!! | TCTTGTTTTTTTGTATCTCC+TGG | - | chr7.1:34605668-34605687 | None:intergenic | 30.0% |
| AAGAAAGACCAACAATGAAG+AGG | + | chr7.1:34606345-34606364 | MS.gene93665:CDS | 35.0% | |
| GTGGATATTACACTTGATGT+TGG | - | chr7.1:34605567-34605586 | None:intergenic | 35.0% | |
| TAAAGATGTCACCAGACTAA+AGG | + | chr7.1:34606138-34606157 | MS.gene93665:CDS | 35.0% | |
| TGATAAAGCAAAAGCCAAAG+AGG | + | chr7.1:34606102-34606121 | MS.gene93665:CDS | 35.0% | |
| TGTGCTTATGTAAGTCCAAT+GGG | + | chr7.1:34605621-34605640 | MS.gene93665:CDS | 35.0% | |
| TTAACAGATAACAGCGAACA+TGG | + | chr7.1:34605925-34605944 | MS.gene93665:intron | 35.0% | |
| TTGTGCTTATGTAAGTCCAA+TGG | + | chr7.1:34605620-34605639 | MS.gene93665:CDS | 35.0% | |
| ! | CTTTAAGAGCCTTAGAAAGA+AGG | + | chr7.1:34606069-34606088 | MS.gene93665:CDS | 35.0% |
| ! | GCTTTTGCTTTATCAACAAC+AGG | - | chr7.1:34606097-34606116 | None:intergenic | 35.0% |
| ! | TGTTGTAAAGTCTTTTGCAG+TGG | - | chr7.1:34605586-34605605 | None:intergenic | 35.0% |
| ! | TGTTTGAAGCGTGACAATTT+GGG | - | chr7.1:34605699-34605718 | None:intergenic | 35.0% |
| !! | TGCTGACATGTTTTGTTCAT+AGG | - | chr7.1:34606311-34606330 | None:intergenic | 35.0% |
| !!! | AATTTTTTCTGCCTCCTCTT+TGG | - | chr7.1:34606119-34606138 | None:intergenic | 35.0% |
| !!! | AGTTTTTGGAGCAATTCTTG+TGG | + | chr7.1:34606267-34606286 | MS.gene93665:CDS | 35.0% |
| !!! | TTTTTTGTATCTCCTGGTTG+AGG | - | chr7.1:34605662-34605681 | None:intergenic | 35.0% |
| AAACTACTGTAAAGGTCAGC+AGG | - | chr7.1:34606207-34606226 | None:intergenic | 40.0% | |
| AACGCCGAGTTTGAAAAGAT+TGG | + | chr7.1:34605788-34605807 | MS.gene93665:CDS | 40.0% | |
| AGAGCGTGAAAACGAATTAG+CGG | + | chr7.1:34606003-34606022 | MS.gene93665:CDS | 40.0% | |
| ATTCCTCGAATTCTAGCATC+TGG | - | chr7.1:34605955-34605974 | None:intergenic | 40.0% | |
| CAACAGGTACCTTCTTTCTA+AGG | - | chr7.1:34606081-34606100 | None:intergenic | 40.0% | |
| GAGTTTGAAGGAAGGATGTA+GGG | - | chr7.1:34605543-34605562 | None:intergenic | 40.0% | |
| TAAAGCAAAAGCCAAAGAGG+AGG | + | chr7.1:34606105-34606124 | MS.gene93665:CDS | 40.0% | |
| TGAGTTTGAAGGAAGGATGT+AGG | - | chr7.1:34605544-34605563 | None:intergenic | 40.0% | |
| ! | ACTTTTCTCCAATCAGTCGA+AGG | - | chr7.1:34606244-34606263 | None:intergenic | 40.0% |
| ! | AGCGGAGTTTGAAGTTCAAA+TGG | + | chr7.1:34606021-34606040 | MS.gene93665:CDS | 40.0% |
| ! | ATGTTGGTGAGTTTGAAGGA+AGG | - | chr7.1:34605551-34605570 | None:intergenic | 40.0% |
| ! | CTTGATGTTGGTGAGTTTGA+AGG | - | chr7.1:34605555-34605574 | None:intergenic | 40.0% |
| ! | GTGTTTGAAGCGTGACAATT+TGG | - | chr7.1:34605700-34605719 | None:intergenic | 40.0% |
| AAGTGGAAGCAAGCATACTC+TGG | - | chr7.1:34605849-34605868 | None:intergenic | 45.0% | |
| AGCTTGAGAATCAGACCCAT+TGG | - | chr7.1:34605639-34605658 | None:intergenic | 45.0% | |
| ATGGAAAACGGCAGAAACGA+AGG | + | chr7.1:34606040-34606059 | MS.gene93665:CDS | 45.0% | |
| TGACATGGATAGCTCGAAAG+TGG | - | chr7.1:34605866-34605885 | None:intergenic | 45.0% | |
| TGGAAAACGGCAGAAACGAA+GGG | + | chr7.1:34606041-34606060 | MS.gene93665:CDS | 45.0% | |
| ACCAGACTAAAGGCTGGTAG+CGG | + | chr7.1:34606148-34606167 | MS.gene93665:CDS | 50.0% | |
| GAGCCAGATGCTAGAATTCG+AGG | + | chr7.1:34605949-34605968 | MS.gene93665:CDS | 50.0% | |
| GATGTCACCAGACTAAAGGC+TGG | + | chr7.1:34606142-34606161 | MS.gene93665:CDS | 50.0% | |
| GATTCTCAAGCTCCTCAACC+AGG | + | chr7.1:34605647-34605666 | MS.gene93665:CDS | 50.0% | |
| TCAGCAGGCCAATGAAGAAG+AGG | - | chr7.1:34606192-34606211 | None:intergenic | 50.0% | |
| !! | TAAAGGCTGGTAGCGGAATC+AGG | + | chr7.1:34606155-34606174 | MS.gene93665:CDS | 50.0% |
| TCCGCTACCAGCCTTTAGTC+TGG | - | chr7.1:34606152-34606171 | None:intergenic | 55.0% | |
| TTGCGTCGCCTTCGACTGAT+TGG | + | chr7.1:34606233-34606252 | MS.gene93665:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 34605524 | 34606378 | 34605524 | ID=MS.gene93665 |
| chr7.1 | mRNA | 34605524 | 34606378 | 34605524 | ID=MS.gene93665.t1;Parent=MS.gene93665 |
| chr7.1 | exon | 34605524 | 34605850 | 34605524 | ID=MS.gene93665.t1.exon1;Parent=MS.gene93665.t1 |
| chr7.1 | CDS | 34605524 | 34605850 | 34605524 | ID=cds.MS.gene93665.t1;Parent=MS.gene93665.t1 |
| chr7.1 | exon | 34605932 | 34606378 | 34605932 | ID=MS.gene93665.t1.exon2;Parent=MS.gene93665.t1 |
| chr7.1 | CDS | 34605932 | 34606378 | 34605932 | ID=cds.MS.gene93665.t1;Parent=MS.gene93665.t1 |
| Gene Sequence |
| Protein sequence |
