AlfalfaGEDB | MS.gene96246

M. sativa cultivar XinJiangDaYe / MS.gene96246

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00646.36 PF01344.28 PF13964.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96246.t1	XP_003593908.1	97	405	12	0	1	405	1	405	3.40E-237	830.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96246.t1	Q9C6Z0	66.5	406	127	2	1	405	1	398	2.3e-165	583.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96246.t1	G7IP74	97.0	405	12	0	1	405	1	405	2.5e-237	830.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049435	MS.gene96246	0.801529	8.62E-49	-1.69E-46
MS.gene050119	MS.gene96246	0.812694	3.73E-51	-1.69E-46
MS.gene05016	MS.gene96246	0.810331	1.22E-50	-1.69E-46
MS.gene050442	MS.gene96246	0.802905	4.49E-49	-1.69E-46
MS.gene050559	MS.gene96246	0.824246	8.96E-54	-1.69E-46
MS.gene050561	MS.gene96246	0.82229	2.56E-53	-1.69E-46
MS.gene050562	MS.gene96246	0.81252	4.07E-51	-1.69E-46
MS.gene050680	MS.gene96246	0.834114	3.61E-56	-1.69E-46
MS.gene050750	MS.gene96246	0.802213	6.24E-49	-1.69E-46
MS.gene050953	MS.gene96246	0.807017	6.21E-50	-1.69E-46
MS.gene052190	MS.gene96246	0.832197	1.08E-55	-1.69E-46
MS.gene052208	MS.gene96246	0.82264	2.13E-53	-1.69E-46
MS.gene052239	MS.gene96246	-0.804864	1.76E-49	-1.69E-46
MS.gene052333	MS.gene96246	0.82756	1.46E-54	-1.69E-46
MS.gene052519	MS.gene96246	0.81317	2.93E-51	-1.69E-46
MS.gene053006	MS.gene96246	0.84136	4.98E-58	-1.69E-46
MS.gene053166	MS.gene96246	0.83134	1.76E-55	-1.69E-46
MS.gene053998	MS.gene96246	0.838478	2.81E-57	-1.69E-46
MS.gene05415	MS.gene96246	0.837886	3.99E-57	-1.69E-46
MS.gene054405	MS.gene96246	0.807203	5.67E-50	-1.69E-46
MS.gene054559	MS.gene96246	0.825903	3.64E-54	-1.69E-46
MS.gene054651	MS.gene96246	0.806677	7.32E-50	-1.69E-46
MS.gene05510	MS.gene96246	0.818902	1.54E-52	-1.69E-46
MS.gene055227	MS.gene96246	0.809292	2.03E-50	-1.69E-46
MS.gene055230	MS.gene96246	0.813366	2.65E-51	-1.69E-46
MS.gene055356	MS.gene96246	0.810027	1.41E-50	-1.69E-46
MS.gene055701	MS.gene96246	0.818244	2.17E-52	-1.69E-46
MS.gene055818	MS.gene96246	0.858442	8.22E-63	-1.69E-46
MS.gene056174	MS.gene96246	0.801246	9.86E-49	-1.69E-46
MS.gene056506	MS.gene96246	0.812762	3.60E-51	-1.69E-46
MS.gene057060	MS.gene96246	0.819871	9.25E-53	-1.69E-46
MS.gene057208	MS.gene96246	0.806542	7.82E-50	-1.69E-46
MS.gene057693	MS.gene96246	0.806798	6.90E-50	-1.69E-46
MS.gene057869	MS.gene96246	0.806436	8.24E-50	-1.69E-46
MS.gene058009	MS.gene96246	0.80193	7.13E-49	-1.69E-46
MS.gene058010	MS.gene96246	0.803378	3.59E-49	-1.69E-46
MS.gene058625	MS.gene96246	0.816383	5.69E-52	-1.69E-46
MS.gene058783	MS.gene96246	0.808215	3.46E-50	-1.69E-46
MS.gene058979	MS.gene96246	0.822318	2.53E-53	-1.69E-46
MS.gene060011	MS.gene96246	0.81924	1.29E-52	-1.69E-46
MS.gene060322	MS.gene96246	0.830583	2.71E-55	-1.69E-46
MS.gene060843	MS.gene96246	0.805718	1.17E-49	-1.69E-46
MS.gene060918	MS.gene96246	0.83859	2.63E-57	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene96246	MS.gene40160	PPI
MS.gene96246	MS.gene028573	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96246.t1	MTR_2g019230	97.037	405	12	0	1	405	1	405	0.0	820
MS.gene96246.t1	MTR_3g108860	33.880	366	214	7	42	405	7	346	3.67e-67	217
MS.gene96246.t1	MTR_4g114110	33.566	286	175	8	44	325	73	347	3.30e-42	154
MS.gene96246.t1	MTR_8g085600	31.834	289	182	8	49	333	74	351	3.95e-38	143
MS.gene96246.t1	MTR_3g073640	27.536	345	178	12	52	330	43	381	9.75e-31	123
MS.gene96246.t1	MTR_8g030940	30.031	323	205	11	51	364	24	334	4.81e-30	119
MS.gene96246.t1	MTR_2g008440	28.205	351	171	13	52	330	43	384	1.57e-29	119
MS.gene96246.t1	MTR_8g080370	30.263	304	186	9	45	334	34	325	1.43e-28	115
MS.gene96246.t1	MTR_3g085570	27.682	289	195	7	49	334	32	309	1.27e-23	101
MS.gene96246.t1	MTR_3g094850	30.272	294	176	11	52	338	9	280	7.89e-23	99.0
MS.gene96246.t1	MTR_2g094093	26.377	345	230	9	50	386	18	346	1.18e-20	92.8
MS.gene96246.t1	MTR_8g085650	30.357	224	140	4	52	262	7	227	9.53e-19	87.0
MS.gene96246.t1	MTR_1330s0010	23.120	359	226	12	12	354	80	404	8.20e-18	85.1
MS.gene96246.t1	MTR_0345s0010	23.120	359	226	12	12	354	67	391	1.03e-17	85.1
MS.gene96246.t1	MTR_0105s0170	23.120	359	226	12	12	354	80	404	1.94e-17	84.3
MS.gene96246.t1	MTR_0105s0040	23.120	359	226	12	12	354	165	489	2.37e-17	84.3
MS.gene96246.t1	MTR_0105s0130	23.120	359	226	12	12	354	67	391	3.58e-17	83.2
MS.gene96246.t1	MTR_0105s0020	23.184	358	227	12	12	354	67	391	8.56e-17	82.0
MS.gene96246.t1	MTR_7g095220	29.954	217	135	7	52	258	4	213	2.00e-16	80.5
MS.gene96246.t1	MTR_6g023805	24.662	296	196	9	53	333	57	340	3.03e-16	80.5
MS.gene96246.t1	MTR_0496s0010	22.513	382	243	14	12	378	49	392	9.93e-16	79.0
MS.gene96246.t1	MTR_4g022260	22.312	372	237	13	12	366	49	385	6.19e-14	73.6
MS.gene96246.t1	MTR_4g108280	22.191	356	241	12	44	379	23	362	3.23e-13	70.9
MS.gene96246.t1	MTR_7g029290	23.256	301	206	9	46	333	43	331	7.15e-12	67.0
MS.gene96246.t1	MTR_7g070320	32.192	146	93	5	156	299	526	667	4.41e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96246.t1	AT1G30090	68.473	406	119	2	1	405	1	398	0.0	599
MS.gene96246.t1	AT2G24540	37.433	374	217	9	35	404	11	371	1.55e-83	260
MS.gene96246.t1	AT1G55270	32.975	279	178	7	49	326	75	345	3.01e-41	151
MS.gene96246.t1	AT1G22040	30.233	344	169	13	52	330	43	380	4.92e-34	132
MS.gene96246.t1	AT1G67480	32.069	290	186	6	42	328	29	310	1.78e-33	129
MS.gene96246.t1	AT1G67480	32.069	290	186	6	42	328	29	310	1.78e-33	129
MS.gene96246.t1	AT1G67480	32.069	290	186	6	42	328	29	310	1.78e-33	129
MS.gene96246.t1	AT1G27420	26.398	322	184	9	51	372	9	277	1.12e-25	107
MS.gene96246.t1	AT1G55270	29.464	224	150	6	104	326	3	219	7.26e-22	95.5
MS.gene96246.t1	AT5G60570	27.365	296	193	7	45	332	39	320	4.81e-21	94.4
MS.gene96246.t1	AT5G60570	27.365	296	193	7	45	332	39	320	4.81e-21	94.4
MS.gene96246.t1	AT5G60570	27.365	296	193	7	45	332	39	320	4.81e-21	94.4
MS.gene96246.t1	AT3G63220	28.472	288	173	10	52	328	4	269	7.81e-20	90.5
MS.gene96246.t1	AT3G63220	28.472	288	173	10	52	328	11	276	8.13e-20	90.5
MS.gene96246.t1	AT3G63220	28.472	288	173	10	52	328	11	276	8.13e-20	90.5
MS.gene96246.t1	AT1G16250	27.530	247	162	7	49	285	5	244	1.34e-18	87.0
MS.gene96246.t1	AT1G16250	27.530	247	162	7	49	285	5	244	1.34e-18	87.0
MS.gene96246.t1	AT3G61350	22.956	318	219	11	52	361	21	320	1.64e-18	86.7
MS.gene96246.t1	AT3G61350	22.956	318	219	11	52	361	21	320	1.64e-18	86.7
MS.gene96246.t1	AT2G21950	27.303	304	194	11	52	344	20	307	3.21e-17	83.2
MS.gene96246.t1	AT4G39580	25.874	286	184	10	56	330	26	294	1.08e-14	75.5
MS.gene96246.t1	AT3G27150	23.256	344	230	10	8	333	21	348	4.24e-14	73.9
MS.gene96246.t1	AT3G27150	23.256	344	230	10	8	333	21	348	4.24e-14	73.9
MS.gene96246.t1	AT1G26930	23.602	322	212	10	52	354	71	377	5.17e-13	70.5
MS.gene96246.t1	AT1G60570	25.342	292	189	11	49	328	17	291	1.21e-12	69.3
MS.gene96246.t1	AT1G80440	29.279	222	143	7	52	262	3	221	6.32e-11	63.9

Find 121 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCCCCTCACAACTTTCTTT+TGG	0.176864	2.2:+65401633	None:intergenic
ATCAGGTAAGCCCGGAATTA+AGG	0.209782	2.2:+65402586	None:intergenic
GGTTCAAGCGTGGTTGTTTA+TGG	0.236597	2.2:-65401855	MS.gene96246:CDS
TTGTCCTTGCAAGGCATTGC+AGG	0.324010	2.2:+65402335	None:intergenic
TGTCGTAGGCCGGAATCTTC+TGG	0.330235	2.2:-65401685	MS.gene96246:CDS
AAGAAAGTTGTGAGGGGAAA+TGG	0.336256	2.2:-65401629	MS.gene96246:CDS
TGACACTATCTCCCAAGTTT+AGG	0.343062	2.2:-65402676	MS.gene96246:CDS
AGCCATAGTTTCCCAGCTAT+TGG	0.348050	2.2:+65401902	None:intergenic
TGCCAACATGGGAACCAATA+TGG	0.351045	2.2:-65402027	MS.gene96246:CDS
TAATTGTCTTCTCCGTCTTC+CGG	0.361345	2.2:-65402552	MS.gene96246:CDS
CTTCCCAGGAATCGGCTTCC+TGG	0.361899	2.2:+65401781	None:intergenic
AGGCAAAGGGGAGCCATCTA+TGG	0.368132	2.2:+65401758	None:intergenic
TAGATCCGATTAGCGGGAAT+TGG	0.368843	2.2:-65402058	MS.gene96246:CDS
CCTCTAGGAGAGACATAAAA+AGG	0.369553	2.2:+65401945	None:intergenic
AGAGCTTGAAGGGGTCAAAT+TGG	0.369698	2.2:+65401560	None:intergenic
AACCAATAGCTGGGAAACTA+TGG	0.377206	2.2:-65401904	MS.gene96246:CDS
AGAGAAGAACCCTTAATTCC+GGG	0.383870	2.2:-65402596	MS.gene96246:CDS
AAGAGAAGAACCCTTAATTC+CGG	0.391873	2.2:-65402597	MS.gene96246:CDS
GTTCAGAACTGCAGCATCAT+AGG	0.399835	2.2:+65402001	None:intergenic
ATAACCAGGAAGCCGATTCC+TGG	0.405175	2.2:-65401785	MS.gene96246:CDS
GTTTCCCAGCTATTGGTTCT+CGG	0.422970	2.2:+65401909	None:intergenic
GCGCGCCAATTCCCGCTAAT+CGG	0.425020	2.2:+65402053	None:intergenic
AGCATCATAGGATGCCATAT+TGG	0.428156	2.2:+65402013	None:intergenic
TCAGGTAAGCCCGGAATTAA+GGG	0.433050	2.2:+65402587	None:intergenic
TGCATATTTGCTCAGGCAAA+GGG	0.438541	2.2:+65401745	None:intergenic
GCTCGAAAGAATGAAGCTTA+AGG	0.440805	2.2:-65401811	MS.gene96246:CDS
TCACAAGCATTCACAGCAAA+AGG	0.445454	2.2:+65401720	None:intergenic
CAACAGCAACCAGAAGATTC+CGG	0.446885	2.2:+65401676	None:intergenic
TTATGTCTCTCCTAGAGGAC+AGG	0.451993	2.2:-65401940	MS.gene96246:CDS
TTTGACCCCTTCAAGCTCTC+AGG	0.453320	2.2:-65401556	MS.gene96246:CDS
AACCACCGCTAACCTAAACT+TGG	0.453592	2.2:+65402664	None:intergenic
AAAGGTTTGCATATTTGCTC+AGG	0.456311	2.2:+65401738	None:intergenic
CGTGGATTGTCCTCTTGACT+TGG	0.459145	2.2:-65402234	MS.gene96246:CDS
TTTGCATATCATAAGTGTAC+TGG	0.463348	2.2:-65402410	MS.gene96246:CDS
AAGGACAAGGTTTGTCCTCA+TGG	0.464137	2.2:-65402320	MS.gene96246:CDS
ATGATGCTGCAGTTCTGAAC+GGG	0.464281	2.2:-65401998	MS.gene96246:CDS
AAATGGGTTTCAAAGATCCT+TGG	0.469304	2.2:-65402442	MS.gene96246:CDS
ATCTATGGCTTCCCAGGAAT+CGG	0.471739	2.2:+65401773	None:intergenic
GATTCCTGGGAAGCCATAGA+TGG	0.472750	2.2:-65401771	MS.gene96246:CDS
CCTCTTGCAGACAGCCCTAC+AGG	0.472883	2.2:+65402514	None:intergenic
GTCAAATTTATGTCGTAGGC+CGG	0.477828	2.2:-65401695	MS.gene96246:CDS
ATCTCCCAAGTTTAGGTTAG+CGG	0.477981	2.2:-65402669	MS.gene96246:CDS
TACTGGAAAGATTCAATGGC+AGG	0.482203	2.2:-65402393	MS.gene96246:CDS
TTGTAACAGAAGGATGGTTA+TGG	0.483194	2.2:-65401968	MS.gene96246:CDS
CAATTAAGAGCAACATCATC+AGG	0.483657	2.2:+65402569	None:intergenic
CGGAATCTTCTGGTTGCTGT+TGG	0.483823	2.2:-65401675	MS.gene96246:CDS
GGAGCCATCTATGGCTTCCC+AGG	0.485903	2.2:+65401767	None:intergenic
TAGTACTGATCTTTATGAGC+TGG	0.486572	2.2:-65402096	MS.gene96246:CDS
ACGATCCGAGAACCAATAGC+TGG	0.496530	2.2:-65401914	MS.gene96246:CDS
TTTACTAATAGGAAGCAAAT+GGG	0.500450	2.2:-65402458	MS.gene96246:CDS
AATAGCACCTGAGAGCTTGA+AGG	0.504117	2.2:+65401549	None:intergenic
TTGCATATTTGCTCAGGCAA+AGG	0.504929	2.2:+65401744	None:intergenic
CTTGCAAGGCATTGCAGGGA+TGG	0.507543	2.2:+65402340	None:intergenic
AGCTGGGAAACTATGGCTGT+TGG	0.507613	2.2:-65401897	MS.gene96246:CDS
GATGGGATGGTTTATGCAGC+TGG	0.507844	2.2:-65402125	MS.gene96246:CDS
CAGGATGAATGACTTTGAAC+CGG	0.508558	2.2:+65402533	None:intergenic
TGCCATATTGGTTCCCATGT+TGG	0.508940	2.2:+65402025	None:intergenic
TGCAATGCCTTGCAAGGACA+AGG	0.510866	2.2:-65402333	MS.gene96246:CDS
ATGGGATGGTTTATGCAGCT+GGG	0.512009	2.2:-65402124	MS.gene96246:CDS
AGGTATTAGATCCGATTAGC+GGG	0.521082	2.2:-65402064	MS.gene96246:CDS
TTGGGAGATAGTGTCATTTG+AGG	0.521340	2.2:+65402683	None:intergenic
AATGGAACTTCAGTGTTCAA+TGG	0.527552	2.2:-65401611	MS.gene96246:CDS
AGTGTACTGGAAAGATTCAA+TGG	0.529957	2.2:-65402397	MS.gene96246:CDS
ATAGCACCTGAGAGCTTGAA+GGG	0.534517	2.2:+65401550	None:intergenic
GCTCAGAAACAACAAATAAG+TGG	0.537113	2.2:+65401832	None:intergenic
TAACCAGGAAGCCGATTCCT+GGG	0.538113	2.2:-65401784	MS.gene96246:CDS
CTTAGAGAATGATCTAACTC+TGG	0.539641	2.2:+65402617	None:intergenic
TTTGCCAGTGGAGTAATCGA+TGG	0.542803	2.2:-65402143	MS.gene96246:CDS
CAAGCACCGGTTCACAAGCT+AGG	0.543317	2.2:-65402710	MS.gene96246:CDS
TTGCCAGTGGAGTAATCGAT+GGG	0.543911	2.2:-65402142	MS.gene96246:CDS
AAGAGGTGGCATATGTTACT+TGG	0.546808	2.2:-65402497	MS.gene96246:CDS
GGACTTAGAGAAGGGTGGAC+TGG	0.550423	2.2:-65401876	MS.gene96246:CDS
TTGGCGCGCTATTGCCAACA+TGG	0.550527	2.2:-65402039	MS.gene96246:CDS
GGATCACCTAGCTTGTGAAC+CGG	0.553072	2.2:+65402704	None:intergenic
GCAACATCATCAGGTAAGCC+CGG	0.556029	2.2:+65402578	None:intergenic
TCAAAGTCATTCATCCTGTA+GGG	0.557607	2.2:-65402528	MS.gene96246:CDS
GGATCGTAGACCTGTCCTCT+AGG	0.560032	2.2:+65401930	None:intergenic
CATCCCTGCAATGCCTTGCA+AGG	0.560526	2.2:-65402339	MS.gene96246:CDS
GATTGTCAAATTTATGTCGT+AGG	0.561359	2.2:-65401699	MS.gene96246:CDS
GAATGATCTAACTCTGGCAT+CGG	0.561877	2.2:+65402623	None:intergenic
CATCCCATCGATTACTCCAC+TGG	0.564791	2.2:+65402139	None:intergenic
TGTCCTTGCAAGGCATTGCA+GGG	0.566024	2.2:+65402336	None:intergenic
TCTTTACGTATGCGGTGGCA+TGG	0.567533	2.2:-65402264	MS.gene96246:CDS
AACCCAAAAGAAAGTTGTGA+GGG	0.567884	2.2:-65401636	MS.gene96246:CDS
TTCAAAGTCATTCATCCTGT+AGG	0.569327	2.2:-65402529	MS.gene96246:CDS
TATGATGCTGCAGTTCTGAA+CGG	0.569619	2.2:-65401999	MS.gene96246:CDS
GTAGGGCTGTCTGCAAGAGG+TGG	0.571680	2.2:-65402511	MS.gene96246:CDS
ATGGCTGTTGGACTTAGAGA+AGG	0.574595	2.2:-65401885	MS.gene96246:CDS
CCTGTAGGGCTGTCTGCAAG+AGG	0.578172	2.2:-65402514	MS.gene96246:CDS
CAGTGGAGTAATCGATGGGA+TGG	0.581579	2.2:-65402138	MS.gene96246:CDS
TGTTACTTGGTAGTAAAGAG+AGG	0.594306	2.2:-65402484	MS.gene96246:CDS
GGACAAACCTTGTCCTTGCA+AGG	0.597418	2.2:+65402326	None:intergenic
TGGGATGGTTTATGCAGCTG+GGG	0.601401	2.2:-65402123	MS.gene96246:CDS
AATGACTTTGAACCGGAAGA+CGG	0.603601	2.2:+65402540	None:intergenic
GAAGCTTAAGGTTTATAACC+AGG	0.604476	2.2:-65401799	MS.gene96246:CDS
TCCCAAGTTTAGGTTAGCGG+TGG	0.605858	2.2:-65402666	MS.gene96246:CDS
TTATGAGCTGGATTCAGCTG+AGG	0.606127	2.2:-65402084	MS.gene96246:CDS
TATTTCAACACCAAGTCAAG+AGG	0.607388	2.2:+65402224	None:intergenic
CGGTGGCATGGTCTCTGACG+TGG	0.609464	2.2:-65402252	MS.gene96246:CDS
GAGGTATTAGATCCGATTAG+CGG	0.618113	2.2:-65402065	MS.gene96246:CDS
GGGAAACTGCTTGTAACAGA+AGG	0.619168	2.2:-65401978	MS.gene96246:CDS
CGATCCGAGAACCAATAGCT+GGG	0.621734	2.2:-65401913	MS.gene96246:CDS
TGGCTGTTGGACTTAGAGAA+GGG	0.623432	2.2:-65401884	MS.gene96246:CDS
AACTGCTTGTAACAGAAGGA+TGG	0.627353	2.2:-65401974	MS.gene96246:CDS
TGGCGCGCTATTGCCAACAT+GGG	0.632332	2.2:-65402038	MS.gene96246:CDS
TGAATCGAATGATCAGTGCT+AGG	0.644133	2.2:-65402172	MS.gene96246:CDS
CTGTTGGACTTAGAGAAGGG+TGG	0.655252	2.2:-65401881	MS.gene96246:CDS
GGTAATCTTTACGTATGCGG+TGG	0.656082	2.2:-65402269	MS.gene96246:CDS
TGTGTTTCGATGCCTCACGA+TGG	0.656289	2.2:-65402290	MS.gene96246:CDS
ACCACCGCTAACCTAAACTT+GGG	0.656767	2.2:+65402665	None:intergenic
GCATATTTGCTCAGGCAAAG+GGG	0.660049	2.2:+65401746	None:intergenic
ACGTAAAGATTACCATCGTG+AGG	0.668276	2.2:+65402278	None:intergenic
AGGGTGGACTGGTTCAAGCG+TGG	0.672213	2.2:-65401865	MS.gene96246:CDS
GGGATGGTTTATGCAGCTGG+GGG	0.673599	2.2:-65402122	MS.gene96246:CDS
GAACCCAAAAGAAAGTTGTG+AGG	0.676488	2.2:-65401637	MS.gene96246:CDS
ACTGATCATTCGATTCATCA+CGG	0.677591	2.2:+65402178	None:intergenic
GGATGGTTTATGCAGCTGGG+GGG	0.678556	2.2:-65402121	MS.gene96246:CDS
ACCCAAAAGAAAGTTGTGAG+GGG	0.696628	2.2:-65401635	MS.gene96246:CDS
TAGCACCTGAGAGCTTGAAG+GGG	0.706757	2.2:+65401551	None:intergenic
GATGGTAATCTTTACGTATG+CGG	0.716864	2.2:-65402272	MS.gene96246:CDS
GAAACACACCGAAAACCATG+AGG	0.732405	2.2:+65402305	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTTACTAATAGGAAGCAAAT+GGG	-	chr2.2:65401821-65401840	MS.gene96246:CDS	25.0%
!!	GTCAAATTGGATAAACTTTT+AGG	+	chr2.2:65402709-65402728	None:intergenic	25.0%
!!	TTTTACTAATAGGAAGCAAA+TGG	-	chr2.2:65401820-65401839	MS.gene96246:CDS	25.0%
!!!	AAGAGAGGTTTTTTACTAAT+AGG	-	chr2.2:65401810-65401829	MS.gene96246:CDS	25.0%
	GATTGTCAAATTTATGTCGT+AGG	-	chr2.2:65402580-65402599	MS.gene96246:CDS	30.0%
	TATGCAAATACAAAAAGCCA+AGG	+	chr2.2:65401857-65401876	None:intergenic	30.0%
	TTTGCATATCATAAGTGTAC+TGG	-	chr2.2:65401869-65401888	MS.gene96246:CDS	30.0%
!!	TTGATCTAACTCACTTTTCT+TGG	-	chr2.2:65401912-65401931	MS.gene96246:CDS	30.0%
	AAAGGTTTGCATATTTGCTC+AGG	+	chr2.2:65402544-65402563	None:intergenic	35.0%
	AACCCAAAAGAAAGTTGTGA+GGG	-	chr2.2:65402643-65402662	MS.gene96246:CDS	35.0%
	AAGAGAAGAACCCTTAATTC+CGG	-	chr2.2:65401682-65401701	MS.gene96246:CDS	35.0%
	AATGGAACTTCAGTGTTCAA+TGG	-	chr2.2:65402668-65402687	MS.gene96246:CDS	35.0%
	ACTGATCATTCGATTCATCA+CGG	+	chr2.2:65402104-65402123	None:intergenic	35.0%
	AGTGTACTGGAAAGATTCAA+TGG	-	chr2.2:65401882-65401901	MS.gene96246:CDS	35.0%
	ATGAGATCACGAAAAATCGT+TGG	-	chr2.2:65402077-65402096	MS.gene96246:CDS	35.0%
	CAATTAAGAGCAACATCATC+AGG	+	chr2.2:65401713-65401732	None:intergenic	35.0%
	CTTAGAGAATGATCTAACTC+TGG	+	chr2.2:65401665-65401684	None:intergenic	35.0%
	GAAGCTTAAGGTTTATAACC+AGG	-	chr2.2:65402480-65402499	MS.gene96246:CDS	35.0%
	GATGGTAATCTTTACGTATG+CGG	-	chr2.2:65402007-65402026	MS.gene96246:CDS	35.0%
	GCTCAGAAACAACAAATAAG+TGG	+	chr2.2:65402450-65402469	None:intergenic	35.0%
	TATTTCAACACCAAGTCAAG+AGG	+	chr2.2:65402058-65402077	None:intergenic	35.0%
	TCAAAGTCATTCATCCTGTA+GGG	-	chr2.2:65401751-65401770	MS.gene96246:CDS	35.0%
	TTCAAAGTCATTCATCCTGT+AGG	-	chr2.2:65401750-65401769	MS.gene96246:CDS	35.0%
	TTGTAACAGAAGGATGGTTA+TGG	-	chr2.2:65402311-65402330	MS.gene96246:CDS	35.0%
!	AAATGGGTTTCAAAGATCCT+TGG	-	chr2.2:65401837-65401856	MS.gene96246:CDS	35.0%
!	TGTTACTTGGTAGTAAAGAG+AGG	-	chr2.2:65401795-65401814	MS.gene96246:CDS	35.0%
!!	TAGTACTGATCTTTATGAGC+TGG	-	chr2.2:65402183-65402202	MS.gene96246:CDS	35.0%
!!!	ACTTTCTTTTGGGTTCAGTA+TGG	+	chr2.2:65402638-65402657	None:intergenic	35.0%
	AACCAATAGCTGGGAAACTA+TGG	-	chr2.2:65402375-65402394	MS.gene96246:CDS	40.0%
	AACTGCTTGTAACAGAAGGA+TGG	-	chr2.2:65402305-65402324	MS.gene96246:CDS	40.0%
	AAGAAAGTTGTGAGGGGAAA+TGG	-	chr2.2:65402650-65402669	MS.gene96246:CDS	40.0%
	AAGAGGTGGCATATGTTACT+TGG	-	chr2.2:65401782-65401801	MS.gene96246:CDS	40.0%
	AATGACTTTGAACCGGAAGA+CGG	+	chr2.2:65401742-65401761	None:intergenic	40.0%
	ACCCAAAAGAAAGTTGTGAG+GGG	-	chr2.2:65402644-65402663	MS.gene96246:CDS	40.0%
	ACGTAAAGATTACCATCGTG+AGG	+	chr2.2:65402004-65402023	None:intergenic	40.0%
	AGAGAAGAACCCTTAATTCC+GGG	-	chr2.2:65401683-65401702	MS.gene96246:CDS	40.0%
	AGCATCATAGGATGCCATAT+TGG	+	chr2.2:65402269-65402288	None:intergenic	40.0%
	AGGTATTAGATCCGATTAGC+GGG	-	chr2.2:65402215-65402234	MS.gene96246:CDS	40.0%
	CAGGATGAATGACTTTGAAC+CGG	+	chr2.2:65401749-65401768	None:intergenic	40.0%
	GAACCCAAAAGAAAGTTGTG+AGG	-	chr2.2:65402642-65402661	MS.gene96246:CDS	40.0%
	GAATGATCTAACTCTGGCAT+CGG	+	chr2.2:65401659-65401678	None:intergenic	40.0%
	GCTCGAAAGAATGAAGCTTA+AGG	-	chr2.2:65402468-65402487	MS.gene96246:CDS	40.0%
	GTCAAATTTATGTCGTAGGC+CGG	-	chr2.2:65402584-65402603	MS.gene96246:CDS	40.0%
	TAATTGTCTTCTCCGTCTTC+CGG	-	chr2.2:65401727-65401746	MS.gene96246:CDS	40.0%
	TACTGGAAAGATTCAATGGC+AGG	-	chr2.2:65401886-65401905	MS.gene96246:CDS	40.0%
	TCACAAGCATTCACAGCAAA+AGG	+	chr2.2:65402562-65402581	None:intergenic	40.0%
	TGAATCGAATGATCAGTGCT+AGG	-	chr2.2:65402107-65402126	MS.gene96246:CDS	40.0%
	TGACACTATCTCCCAAGTTT+AGG	-	chr2.2:65401603-65401622	MS.gene96246:CDS	40.0%
	TGCATATTTGCTCAGGCAAA+GGG	+	chr2.2:65402537-65402556	None:intergenic	40.0%
	TTGCATATTTGCTCAGGCAA+AGG	+	chr2.2:65402538-65402557	None:intergenic	40.0%
!	AGGTTTGTCCTCATGGTTTT+CGG	-	chr2.2:65401966-65401985	MS.gene96246:CDS	40.0%
!	ATCTCCCAAGTTTAGGTTAG+CGG	-	chr2.2:65401610-65401629	MS.gene96246:CDS	40.0%
!	ATTTTCGTCTCAACAAGCAC+CGG	-	chr2.2:65401556-65401575	MS.gene96246:CDS	40.0%
!	CCTCTAGGAGAGACATAAAA+AGG	+	chr2.2:65402337-65402356	None:intergenic	40.0%
!	CCTTTTTATGTCTCTCCTAG+AGG	-	chr2.2:65402334-65402353	MS.gene96246:CDS	40.0%
!	GAGGTATTAGATCCGATTAG+CGG	-	chr2.2:65402214-65402233	MS.gene96246:CDS	40.0%
!	TCCCCTCACAACTTTCTTTT+GGG	+	chr2.2:65402648-65402667	None:intergenic	40.0%
!	TTCCCCTCACAACTTTCTTT+TGG	+	chr2.2:65402649-65402668	None:intergenic	40.0%
!!	TATGATGCTGCAGTTCTGAA+CGG	-	chr2.2:65402280-65402299	MS.gene96246:CDS	40.0%
!!	TTGGGAGATAGTGTCATTTG+AGG	+	chr2.2:65401599-65401618	None:intergenic	40.0%
	AACCACCGCTAACCTAAACT+TGG	+	chr2.2:65401618-65401637	None:intergenic	45.0%
	AAGGACAAGGTTTGTCCTCA+TGG	-	chr2.2:65401959-65401978	MS.gene96246:CDS	45.0%
	AATAGCACCTGAGAGCTTGA+AGG	+	chr2.2:65402733-65402752	None:intergenic	45.0%
	ACCACCGCTAACCTAAACTT+GGG	+	chr2.2:65401617-65401636	None:intergenic	45.0%
	AGAGCTTGAAGGGGTCAAAT+TGG	+	chr2.2:65402722-65402741	None:intergenic	45.0%
	AGCCATAGTTTCCCAGCTAT+TGG	+	chr2.2:65402380-65402399	None:intergenic	45.0%
	ATAGCACCTGAGAGCTTGAA+GGG	+	chr2.2:65402732-65402751	None:intergenic	45.0%
	ATCAGGTAAGCCCGGAATTA+AGG	+	chr2.2:65401696-65401715	None:intergenic	45.0%
	ATCTATGGCTTCCCAGGAAT+CGG	+	chr2.2:65402509-65402528	None:intergenic	45.0%
	ATGGCTGTTGGACTTAGAGA+AGG	-	chr2.2:65402394-65402413	MS.gene96246:CDS	45.0%
	ATGGGATGGTTTATGCAGCT+GGG	-	chr2.2:65402155-65402174	MS.gene96246:CDS	45.0%
	CAACAGCAACCAGAAGATTC+CGG	+	chr2.2:65402606-65402625	None:intergenic	45.0%
	GAAACACACCGAAAACCATG+AGG	+	chr2.2:65401977-65401996	None:intergenic	45.0%
	GCATATTTGCTCAGGCAAAG+GGG	+	chr2.2:65402536-65402555	None:intergenic	45.0%
	GGGAAACTGCTTGTAACAGA+AGG	-	chr2.2:65402301-65402320	MS.gene96246:CDS	45.0%
	GGTAATCTTTACGTATGCGG+TGG	-	chr2.2:65402010-65402029	MS.gene96246:CDS	45.0%
	GGTTCAAGCGTGGTTGTTTA+TGG	-	chr2.2:65402424-65402443	MS.gene96246:CDS	45.0%
	GTTCAGAACTGCAGCATCAT+AGG	+	chr2.2:65402281-65402300	None:intergenic	45.0%
	TAGATCCGATTAGCGGGAAT+TGG	-	chr2.2:65402221-65402240	MS.gene96246:CDS	45.0%
	TCAGGTAAGCCCGGAATTAA+GGG	+	chr2.2:65401695-65401714	None:intergenic	45.0%
	TGCCAACATGGGAACCAATA+TGG	-	chr2.2:65402252-65402271	MS.gene96246:CDS	45.0%
	TGGCTGTTGGACTTAGAGAA+GGG	-	chr2.2:65402395-65402414	MS.gene96246:CDS	45.0%
	TTATGAGCTGGATTCAGCTG+AGG	-	chr2.2:65402195-65402214	MS.gene96246:CDS	45.0%
	TTATGTCTCTCCTAGAGGAC+AGG	-	chr2.2:65402339-65402358	MS.gene96246:CDS	45.0%
	TTGCCAGTGGAGTAATCGAT+GGG	-	chr2.2:65402137-65402156	MS.gene96246:CDS	45.0%
	TTTGCCAGTGGAGTAATCGA+TGG	-	chr2.2:65402136-65402155	MS.gene96246:CDS	45.0%
!!	ATGATGCTGCAGTTCTGAAC+GGG	-	chr2.2:65402281-65402300	MS.gene96246:CDS	45.0%
!!	GTTTCCCAGCTATTGGTTCT+CGG	+	chr2.2:65402373-65402392	None:intergenic	45.0%
!!	TGCCATATTGGTTCCCATGT+TGG	+	chr2.2:65402257-65402276	None:intergenic	45.0%
!!!	GCTAGGTCATTTTTTGCCAG+TGG	-	chr2.2:65402124-65402143	MS.gene96246:CDS	45.0%
	ACGATCCGAGAACCAATAGC+TGG	-	chr2.2:65402365-65402384	MS.gene96246:CDS	50.0%
	AGCTGGGAAACTATGGCTGT+TGG	-	chr2.2:65402382-65402401	MS.gene96246:CDS	50.0%
	CAGTGGAGTAATCGATGGGA+TGG	-	chr2.2:65402141-65402160	MS.gene96246:CDS	50.0%
	CGATCCGAGAACCAATAGCT+GGG	-	chr2.2:65402366-65402385	MS.gene96246:CDS	50.0%
	CTGTTGGACTTAGAGAAGGG+TGG	-	chr2.2:65402398-65402417	MS.gene96246:CDS	50.0%
	GATGGGATGGTTTATGCAGC+TGG	-	chr2.2:65402154-65402173	MS.gene96246:CDS	50.0%
	GATTCCTGGGAAGCCATAGA+TGG	-	chr2.2:65402508-65402527	MS.gene96246:CDS	50.0%
	GCAACATCATCAGGTAAGCC+CGG	+	chr2.2:65401704-65401723	None:intergenic	50.0%
	GGACAAACCTTGTCCTTGCA+AGG	+	chr2.2:65401956-65401975	None:intergenic	50.0%
	TAGCACCTGAGAGCTTGAAG+GGG	+	chr2.2:65402731-65402750	None:intergenic	50.0%
	TCTTTACGTATGCGGTGGCA+TGG	-	chr2.2:65402015-65402034	MS.gene96246:CDS	50.0%
	TGCAATGCCTTGCAAGGACA+AGG	-	chr2.2:65401946-65401965	MS.gene96246:CDS	50.0%
	TGGGATGGTTTATGCAGCTG+GGG	-	chr2.2:65402156-65402175	MS.gene96246:CDS	50.0%
	TGTGTTTCGATGCCTCACGA+TGG	-	chr2.2:65401989-65402008	MS.gene96246:CDS	50.0%
	TTTGACCCCTTCAAGCTCTC+AGG	-	chr2.2:65402723-65402742	MS.gene96246:CDS	50.0%
!	ATAACCAGGAAGCCGATTCC+TGG	-	chr2.2:65402494-65402513	MS.gene96246:CDS	50.0%
!	CATCCCATCGATTACTCCAC+TGG	+	chr2.2:65402143-65402162	None:intergenic	50.0%
!	CGGAATCTTCTGGTTGCTGT+TGG	-	chr2.2:65402604-65402623	MS.gene96246:CDS	50.0%
!	CGTGGATTGTCCTCTTGACT+TGG	-	chr2.2:65402045-65402064	MS.gene96246:CDS	50.0%
!	GGATCACCTAGCTTGTGAAC+CGG	+	chr2.2:65401578-65401597	None:intergenic	50.0%
!	TAACCAGGAAGCCGATTCCT+GGG	-	chr2.2:65402495-65402514	MS.gene96246:CDS	50.0%
!	TCCCAAGTTTAGGTTAGCGG+TGG	-	chr2.2:65401613-65401632	MS.gene96246:CDS	50.0%
!!	TGTCCTTGCAAGGCATTGCA+GGG	+	chr2.2:65401946-65401965	None:intergenic	50.0%
!!	TTGTCCTTGCAAGGCATTGC+AGG	+	chr2.2:65401947-65401966	None:intergenic	50.0%
	AGGCAAAGGGGAGCCATCTA+TGG	+	chr2.2:65402524-65402543	None:intergenic	55.0%
	CATCCCTGCAATGCCTTGCA+AGG	-	chr2.2:65401940-65401959	MS.gene96246:CDS	55.0%
	GGACTTAGAGAAGGGTGGAC+TGG	-	chr2.2:65402403-65402422	MS.gene96246:CDS	55.0%
	GGATCGTAGACCTGTCCTCT+AGG	+	chr2.2:65402352-65402371	None:intergenic	55.0%
	GGATGGTTTATGCAGCTGGG+GGG	-	chr2.2:65402158-65402177	MS.gene96246:CDS	55.0%
	GGGATGGTTTATGCAGCTGG+GGG	-	chr2.2:65402157-65402176	MS.gene96246:CDS	55.0%
	TGGCGCGCTATTGCCAACAT+GGG	-	chr2.2:65402241-65402260	MS.gene96246:CDS	55.0%
	TGTCGTAGGCCGGAATCTTC+TGG	-	chr2.2:65402594-65402613	MS.gene96246:CDS	55.0%
	TTGGCGCGCTATTGCCAACA+TGG	-	chr2.2:65402240-65402259	MS.gene96246:CDS	55.0%
!!	CAAGCACCGGTTCACAAGCT+AGG	-	chr2.2:65401569-65401588	MS.gene96246:CDS	55.0%
!!	CTTGCAAGGCATTGCAGGGA+TGG	+	chr2.2:65401942-65401961	None:intergenic	55.0%
	CCTCTTGCAGACAGCCCTAC+AGG	+	chr2.2:65401768-65401787	None:intergenic	60.0%
	CCTGTAGGGCTGTCTGCAAG+AGG	-	chr2.2:65401765-65401784	MS.gene96246:CDS	60.0%
	GCGCGCCAATTCCCGCTAAT+CGG	+	chr2.2:65402229-65402248	None:intergenic	60.0%
	GGAGCCATCTATGGCTTCCC+AGG	+	chr2.2:65402515-65402534	None:intergenic	60.0%
	GTAGGGCTGTCTGCAAGAGG+TGG	-	chr2.2:65401768-65401787	MS.gene96246:CDS	60.0%
!	CTTCCCAGGAATCGGCTTCC+TGG	+	chr2.2:65402501-65402520	None:intergenic	60.0%
!!	AGGGTGGACTGGTTCAAGCG+TGG	-	chr2.2:65402414-65402433	MS.gene96246:CDS	60.0%
	CGGTGGCATGGTCTCTGACG+TGG	-	chr2.2:65402027-65402046	MS.gene96246:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	65401542	65402759	65401542	ID=MS.gene96246
chr2.2	mRNA	65401542	65402759	65401542	ID=MS.gene96246.t1;Parent=MS.gene96246
chr2.2	exon	65401542	65402759	65401542	ID=MS.gene96246.t1.exon1;Parent=MS.gene96246.t1
chr2.2	CDS	65401542	65402759	65401542	ID=cds.MS.gene96246.t1;Parent=MS.gene96246.t1

Gene Sequence

>MS.gene96246

ATGCAGCGTGTTAGATTTTCGTCTCAACAAGCACCGGTTCACAAGCTAGGTGATCCTCAAATGACACTATCTCCCAAGTTTAGGTTAGCGGTGGTTCAATCTAACTTGATGAGTCCGATGCCAGAGTTAGATCATTCTCTAAGAGAAGAACCCTTAATTCCGGGCTTACCTGATGATGTTGCTCTTAATTGTCTTCTCCGTCTTCCGGTTCAAAGTCATTCATCCTGTAGGGCTGTCTGCAAGAGGTGGCATATGTTACTTGGTAGTAAAGAGAGGTTTTTTACTAATAGGAAGCAAATGGGTTTCAAAGATCCTTGGCTTTTTGTATTTGCATATCATAAGTGTACTGGAAAGATTCAATGGCAGGTTTTTGATCTAACTCACTTTTCTTGGCACACCATCCCTGCAATGCCTTGCAAGGACAAGGTTTGTCCTCATGGTTTTCGGTGTGTTTCGATGCCTCACGATGGTAATCTTTACGTATGCGGTGGCATGGTCTCTGACGTGGATTGTCCTCTTGACTTGGTGTTGAAATATGAGATCACGAAAAATCGTTGGACCGTGATGAATCGAATGATCAGTGCTAGGTCATTTTTTGCCAGTGGAGTAATCGATGGGATGGTTTATGCAGCTGGGGGGAATAGTACTGATCTTTATGAGCTGGATTCAGCTGAGGTATTAGATCCGATTAGCGGGAATTGGCGCGCTATTGCCAACATGGGAACCAATATGGCATCCTATGATGCTGCAGTTCTGAACGGGAAACTGCTTGTAACAGAAGGATGGTTATGGCCTTTTTATGTCTCTCCTAGAGGACAGGTCTACGATCCGAGAACCAATAGCTGGGAAACTATGGCTGTTGGACTTAGAGAAGGGTGGACTGGTTCAAGCGTGGTTGTTTATGGCCACTTATTTGTTGTTTCTGAGCTCGAAAGAATGAAGCTTAAGGTTTATAACCAGGAAGCCGATTCCTGGGAAGCCATAGATGGCTCCCCTTTGCCTGAGCAAATATGCAAACCTTTTGCTGTGAATGCTTGTGATTGTCAAATTTATGTCGTAGGCCGGAATCTTCTGGTTGCTGTTGGTCATATCTCCATACTGAACCCAAAAGAAAGTTGTGAGGGGAAATGGAACTTCAGTGTTCAATGGCAAGTAATTGACGCGCCTAAAAGTTTATCCAATTTGACCCCTTCAAGCTCTCAGGTGCTATTTGCATAG

Protein sequence

>MS.gene96246.t1

MQRVRFSSQQAPVHKLGDPQMTLSPKFRLAVVQSNLMSPMPELDHSLREEPLIPGLPDDVALNCLLRLPVQSHSSCRAVCKRWHMLLGSKERFFTNRKQMGFKDPWLFVFAYHKCTGKIQWQVFDLTHFSWHTIPAMPCKDKVCPHGFRCVSMPHDGNLYVCGGMVSDVDCPLDLVLKYEITKNRWTVMNRMISARSFFASGVIDGMVYAAGGNSTDLYELDSAEVLDPISGNWRAIANMGTNMASYDAAVLNGKLLVTEGWLWPFYVSPRGQVYDPRTNSWETMAVGLREGWTGSSVVVYGHLFVVSELERMKLKVYNQEADSWEAIDGSPLPEQICKPFAVNACDCQIYVVGRNLLVAVGHISILNPKESCEGKWNFSVQWQVIDAPKSLSNLTPSSSQVLFA