Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003983.01.T01 | XP_003590641.1 | 98.684 | 380 | 5 | 0 | 5 | 384 | 1 | 380 | 0 | 777 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003983.01.T01 | Q93ZQ3 | 70.876 | 388 | 103 | 6 | 3 | 384 | 1 | 384 | 0 | 560 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003983.01.T01 | G7IEC3 | 98.684 | 380 | 5 | 0 | 5 | 384 | 1 | 380 | 0.0 | 777 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048797.01 | MsG0180003983.01 | 0.809311 | 2.014941e-50 | 1.179028e-47 |
| MsG0180003857.01 | MsG0180003983.01 | 0.840386 | 8.972780e-58 | 1.276096e-54 |
| MsG0180003905.01 | MsG0180003983.01 | 0.849319 | 3.489597e-60 | 6.590522e-57 |
| MsG0180003983.01 | MsG0180004120.01 | 0.853083 | 3.020113e-61 | 6.448071e-58 |
| MsG0180003983.01 | MsG0180004185.01 | 0.813419 | 2.584862e-51 | 1.687160e-48 |
| MsG0180003983.01 | MsG0180004538.01 | 0.812886 | 3.383216e-51 | 2.176613e-48 |
| MsG0180003983.01 | MsG0180004583.01 | 0.844557 | 7.022074e-59 | 1.137998e-55 |
| MsG0180003983.01 | MsG0180004652.01 | 0.806194 | 9.260215e-50 | 4.995195e-47 |
| MsG0180003983.01 | MsG0180005655.01 | 0.864594 | 1.086681e-64 | 3.430727e-61 |
| MsG0180003983.01 | MsG0280006851.01 | 0.809836 | 1.553660e-50 | 9.219016e-48 |
| MsG0180003983.01 | MsG0280007688.01 | 0.818238 | 2.175068e-52 | 1.619067e-49 |
| MsG0180003983.01 | MsG0380014542.01 | 0.834875 | 2.327326e-56 | 2.798200e-53 |
| MsG0180003983.01 | MsG0380014544.01 | 0.834710 | 2.561154e-56 | 3.063736e-53 |
| MsG0180003983.01 | MsG0380014666.01 | 0.821771 | 3.382399e-53 | 2.777762e-50 |
| MsG0180003983.01 | MsG0380014807.01 | 0.801727 | 7.855942e-49 | 3.779098e-46 |
| MsG0180003983.01 | MsG0380015340.01 | 0.816069 | 6.685807e-52 | 4.690125e-49 |
| MsG0180003983.01 | MsG0380015656.01 | 0.807279 | 5.463513e-50 | 3.030583e-47 |
| MsG0180003983.01 | MsG0380015954.01 | 0.811622 | 6.384793e-51 | 3.971338e-48 |
| MsG0180003983.01 | MsG0380016027.01 | 0.810454 | 1.143616e-50 | 6.896234e-48 |
| MsG0180003983.01 | MsG0380017128.01 | 0.830917 | 2.241638e-55 | 2.395426e-52 |
| MsG0180003983.01 | MsG0380017332.01 | 0.803675 | 3.113311e-49 | 1.573999e-46 |
| MsG0180003983.01 | MsG0380017696.01 | 0.830885 | 2.282427e-55 | 2.436605e-52 |
| MsG0180003983.01 | MsG0380018055.01 | 0.817782 | 2.758448e-52 | 2.027905e-49 |
| MsG0180003983.01 | MsG0480018228.01 | 0.851104 | 1.102625e-60 | 2.207650e-57 |
| MsG0180003983.01 | MsG0480018767.01 | 0.815326 | 9.791263e-52 | 6.729771e-49 |
| MsG0180003983.01 | MsG0480020398.01 | 0.841738 | 3.961421e-58 | 5.874796e-55 |
| MsG0180003983.01 | MsG0480020856.01 | 0.821421 | 4.074642e-53 | 3.313442e-50 |
| MsG0180003983.01 | MsG0480020937.01 | 0.803370 | 3.601406e-49 | 1.806536e-46 |
| MsG0180003983.01 | MsG0480021100.01 | 0.819292 | 1.254105e-52 | 9.610788e-50 |
| MsG0180003983.01 | MsG0480021773.01 | 0.834701 | 2.573588e-56 | 3.077874e-53 |
| MsG0180003983.01 | MsG0480021902.01 | 0.848930 | 4.477897e-60 | 8.350426e-57 |
| MsG0180003983.01 | MsG0480022307.01 | 0.806397 | 8.391845e-50 | 4.549941e-47 |
| MsG0180003983.01 | MsG0480022378.01 | 0.814777 | 1.296270e-51 | 8.777545e-49 |
| MsG0180003983.01 | MsG0480022682.01 | 0.825358 | 4.895344e-54 | 4.451785e-51 |
| MsG0180003983.01 | MsG0480022784.01 | 0.819148 | 1.352046e-52 | 1.032037e-49 |
| MsG0180003983.01 | MsG0480023414.01 | 0.801937 | 7.113772e-49 | 3.440401e-46 |
| MsG0180003983.01 | MsG0480023426.01 | 0.825021 | 5.881509e-54 | 5.298057e-51 |
| MsG0180003983.01 | MsG0480023706.01 | 0.851488 | 8.590905e-61 | 1.741558e-57 |
| MsG0180003983.01 | MsG0480023842.01 | 0.801494 | 8.770535e-49 | 4.194452e-46 |
| MsG0180003983.01 | MsG0580024276.01 | 0.816117 | 6.525075e-52 | 4.583221e-49 |
| MsG0180003983.01 | MsG0580024787.01 | 0.802724 | 4.898593e-49 | 2.417193e-46 |
| MsG0180003983.01 | MsG0580025504.01 | 0.834367 | 3.122732e-56 | 3.698079e-53 |
| MsG0180003983.01 | MsG0580028955.01 | 0.802239 | 6.165332e-49 | 3.004819e-46 |
| MsG0180003983.01 | MsG0580030141.01 | 0.808763 | 2.639592e-50 | 1.522079e-47 |
| MsG0180003983.01 | MsG0680031006.01 | 0.825007 | 5.926362e-54 | 5.336389e-51 |
| MsG0180003983.01 | MsG0780038736.01 | 0.836595 | 8.534909e-57 | 1.080613e-53 |
| MsG0180003983.01 | MsG0780040830.01 | 0.809565 | 1.776711e-50 | 1.046732e-47 |
| MsG0180003983.01 | MsG0780040899.01 | 0.802067 | 6.687599e-49 | 3.245123e-46 |
| MsG0180003983.01 | MsG0880043684.01 | 0.835218 | 1.907095e-56 | 2.316913e-53 |
| MsG0180003983.01 | MsG0880043714.01 | 0.800720 | 1.262714e-48 | 5.921494e-46 |
| MsG0180003983.01 | MsG0880044437.01 | 0.852081 | 5.834478e-61 | 1.205363e-57 |
| MsG0180003983.01 | MsG0880045356.01 | 0.812250 | 4.659894e-51 | 2.947981e-48 |
| MsG0180003983.01 | MsG0880045439.01 | 0.840130 | 1.046509e-57 | 1.476613e-54 |
| MsG0180003983.01 | MsG0880045584.01 | 0.803389 | 3.568680e-49 | 1.791044e-46 |
| MsG0180003983.01 | MsG0880045612.01 | 0.837699 | 4.456368e-57 | 5.836046e-54 |
| MsG0180003983.01 | MsG0880045647.01 | 0.874533 | 6.268416e-68 | 2.831429e-64 |
| MsG0180003983.01 | MsG0880045879.01 | 0.836326 | 9.994059e-57 | 1.255400e-53 |
| MsG0180003983.01 | MsG0880046904.01 | 0.832057 | 1.174318e-55 | 1.297907e-52 |
| MsG0180003983.01 | MsG0880047649.01 | 0.843819 | 1.108077e-58 | 1.754528e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003983.01.T01 | MTR_1g072190 | 98.684 | 380 | 5 | 0 | 5 | 384 | 1 | 380 | 0.0 | 777 |
| MsG0180003983.01.T01 | MTR_7g094940 | 72.441 | 381 | 97 | 4 | 5 | 379 | 1 | 379 | 0.0 | 545 |
| MsG0180003983.01.T01 | MTR_1g095165 | 40.489 | 368 | 177 | 6 | 33 | 360 | 74 | 439 | 1.29e-88 | 275 |
| MsG0180003983.01.T01 | MTR_2g029510 | 37.799 | 418 | 191 | 11 | 1 | 367 | 20 | 419 | 6.66e-72 | 231 |
| MsG0180003983.01.T01 | MTR_0368s0010 | 44.706 | 255 | 110 | 5 | 135 | 360 | 23 | 275 | 3.23e-64 | 207 |
| MsG0180003983.01.T01 | MTR_5g017670 | 27.362 | 307 | 194 | 12 | 36 | 317 | 18 | 320 | 1.54e-26 | 109 |
| MsG0180003983.01.T01 | MTR_8g007090 | 27.463 | 335 | 211 | 11 | 36 | 347 | 34 | 359 | 6.71e-26 | 108 |
| MsG0180003983.01.T01 | MTR_5g017680 | 26.879 | 346 | 206 | 13 | 36 | 344 | 12 | 347 | 2.05e-25 | 106 |
| MsG0180003983.01.T01 | MTR_1g019940 | 24.927 | 341 | 219 | 11 | 36 | 344 | 23 | 358 | 2.88e-25 | 106 |
| MsG0180003983.01.T01 | MTR_8g007095 | 27.164 | 335 | 212 | 11 | 36 | 347 | 34 | 359 | 1.02e-24 | 105 |
| MsG0180003983.01.T01 | MTR_1g019940 | 24.927 | 341 | 217 | 12 | 36 | 344 | 23 | 356 | 7.42e-24 | 102 |
| MsG0180003983.01.T01 | MTR_8g007065 | 29.218 | 243 | 151 | 7 | 36 | 267 | 34 | 266 | 4.23e-23 | 100 |
| MsG0180003983.01.T01 | MTR_3g101410 | 26.667 | 345 | 206 | 11 | 36 | 344 | 22 | 355 | 4.19e-22 | 97.4 |
| MsG0180003983.01.T01 | MTR_8g007040 | 26.384 | 307 | 195 | 9 | 36 | 321 | 34 | 330 | 7.64e-22 | 96.7 |
| MsG0180003983.01.T01 | MTR_3g101400 | 26.254 | 339 | 215 | 11 | 36 | 344 | 22 | 355 | 8.85e-22 | 96.3 |
| MsG0180003983.01.T01 | MTR_8g007140 | 28.972 | 214 | 135 | 7 | 58 | 267 | 53 | 253 | 1.14e-19 | 90.1 |
| MsG0180003983.01.T01 | MTR_8g007125 | 26.216 | 370 | 231 | 12 | 36 | 382 | 34 | 384 | 8.83e-19 | 87.8 |
| MsG0180003983.01.T01 | MTR_1g019940 | 24.537 | 216 | 135 | 7 | 156 | 344 | 25 | 239 | 3.58e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003983.01.T01 | AT3G63200 | 70.876 | 388 | 103 | 6 | 3 | 384 | 1 | 384 | 0.0 | 560 |
| MsG0180003983.01.T01 | AT2G39220 | 43.296 | 358 | 171 | 5 | 33 | 360 | 107 | 462 | 2.16e-90 | 281 |
| MsG0180003983.01.T01 | AT3G54950 | 40.988 | 405 | 181 | 9 | 5 | 360 | 54 | 449 | 2.34e-87 | 273 |
| MsG0180003983.01.T01 | AT4G29800 | 39.623 | 371 | 171 | 8 | 36 | 360 | 123 | 486 | 7.74e-78 | 249 |
| MsG0180003983.01.T01 | AT4G29800 | 38.978 | 372 | 173 | 8 | 36 | 360 | 123 | 487 | 4.50e-76 | 244 |
| MsG0180003983.01.T01 | AT4G37060 | 32.609 | 276 | 171 | 8 | 36 | 299 | 21 | 293 | 1.01e-38 | 143 |
| MsG0180003983.01.T01 | AT4G37060 | 30.065 | 306 | 170 | 9 | 36 | 299 | 21 | 324 | 8.88e-35 | 133 |
| MsG0180003983.01.T01 | AT4G37070 | 29.181 | 281 | 174 | 7 | 36 | 299 | 21 | 293 | 4.78e-32 | 125 |
| MsG0180003983.01.T01 | AT4G37070 | 29.181 | 281 | 174 | 7 | 36 | 299 | 21 | 293 | 4.78e-32 | 125 |
| MsG0180003983.01.T01 | AT4G37070 | 28.198 | 344 | 204 | 11 | 36 | 345 | 21 | 355 | 7.41e-32 | 125 |
| MsG0180003983.01.T01 | AT4G37070 | 27.758 | 281 | 167 | 7 | 36 | 299 | 21 | 282 | 6.66e-28 | 113 |
| MsG0180003983.01.T01 | AT5G43590 | 27.143 | 350 | 212 | 11 | 36 | 353 | 16 | 354 | 2.48e-26 | 109 |
| MsG0180003983.01.T01 | AT5G43590 | 27.143 | 350 | 212 | 11 | 36 | 353 | 83 | 421 | 6.30e-26 | 108 |
| MsG0180003983.01.T01 | AT2G26560 | 27.129 | 317 | 183 | 11 | 36 | 317 | 21 | 324 | 5.90e-23 | 99.8 |
| MsG0180003983.01.T01 | AT4G37050 | 27.018 | 285 | 162 | 10 | 53 | 302 | 54 | 327 | 7.04e-23 | 99.8 |
Find 93 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACGGTTGTTGACTTTAA+AGG | 0.243570 | 1:+71355168 | MsG0180003983.01.T01:CDS |
| CACGTGCTCAACGCCGTTAA+TGG | 0.278448 | 1:+71355549 | MsG0180003983.01.T01:CDS |
| AAGCCATTTGTGTTCGAGTT+TGG | 0.303079 | 1:-71354759 | None:intergenic |
| GTGCAACGTCTTGTTGCTAA+TGG | 0.308645 | 1:+71355924 | MsG0180003983.01.T01:CDS |
| GAACGGCGTGGAGGATCTTT+TGG | 0.314436 | 1:+71355471 | MsG0180003983.01.T01:CDS |
| TACGGATTACGTCAGAATTC+AGG | 0.347197 | 1:+71355642 | MsG0180003983.01.T01:CDS |
| TTGTAACAGGCGTTTGGTTT+GGG | 0.353889 | 1:+71355801 | MsG0180003983.01.T01:intron |
| TTTGTAACAGGCGTTTGGTT+TGG | 0.357424 | 1:+71355800 | MsG0180003983.01.T01:intron |
| AGAATGCGTTTCCAAGCATT+TGG | 0.389464 | 1:-71355609 | None:intergenic |
| TGAATTCAAAAGGTTTGAAA+TGG | 0.393286 | 1:-71355333 | None:intergenic |
| TTCGACATTGTCGCTGGTAC+CGG | 0.395862 | 1:+71354935 | MsG0180003983.01.T01:CDS |
| GAGCGTGAGGATCGGAGGTT+TGG | 0.410241 | 1:-71354898 | None:intergenic |
| TCAAAAGGTTTGAAATGGTT+AGG | 0.422953 | 1:-71355328 | None:intergenic |
| CTCTACAAACGCAAATCAAC+CGG | 0.426095 | 1:+71355064 | MsG0180003983.01.T01:CDS |
| GCTGTTACATCTCTCGCTAA+TGG | 0.433412 | 1:+71355984 | MsG0180003983.01.T01:CDS |
| TTGAATTCACTTCCGTCGAC+GGG | 0.443523 | 1:+71355347 | MsG0180003983.01.T01:CDS |
| GAGTATTTCGTCGCCGGTGC+CGG | 0.444213 | 1:+71355086 | MsG0180003983.01.T01:CDS |
| AATGCTGAGAATGCGTGTTT+TGG | 0.445862 | 1:-71354807 | None:intergenic |
| GATAATACGAACGGATTCTC+TGG | 0.450254 | 1:-71355023 | None:intergenic |
| TTGGTTCTTTCATTGGGAAA+CGG | 0.453399 | 1:+71355490 | MsG0180003983.01.T01:CDS |
| ATGGTTGACATTGTTCTTGA+CGG | 0.456691 | 1:+71355568 | MsG0180003983.01.T01:CDS |
| CATGACTAAACCACCGTCTA+CGG | 0.459167 | 1:-71355386 | None:intergenic |
| CAAGGACTGAACGGTGGACT+TGG | 0.463958 | 1:-71355951 | None:intergenic |
| AGAACAATGTCAACCATTAA+CGG | 0.466581 | 1:-71355562 | None:intergenic |
| GAGGAAGTGTTGAAGGAAAG+AGG | 0.467634 | 1:+71355831 | MsG0180003983.01.T01:CDS |
| TGCTTGGAAACGCATTCTGT+TGG | 0.468643 | 1:+71355614 | MsG0180003983.01.T01:CDS |
| GAGACTAACGGAAACAGAAT+CGG | 0.469947 | 1:+71355894 | MsG0180003983.01.T01:CDS |
| TCTCCAAACTCGAACACAAA+TGG | 0.477489 | 1:+71354756 | MsG0180003983.01.T01:CDS |
| AAGTGAAGAGGAAGTGTTGA+AGG | 0.478976 | 1:+71355824 | MsG0180003983.01.T01:CDS |
| AGGAAGTGTTGAAGGAAAGA+GGG | 0.480873 | 1:+71355832 | MsG0180003983.01.T01:CDS |
| TCGTTAGAAAAGAAGATTCA+CGG | 0.480915 | 1:+71355152 | MsG0180003983.01.T01:CDS |
| ATCACCGCAGCCGATGCATT+CGG | 0.482402 | 1:+71354983 | MsG0180003983.01.T01:CDS |
| GGGTTGGAGTCGTTACCGTT+TGG | 0.482615 | 1:+71355852 | MsG0180003983.01.T01:CDS |
| TCGACGGAAGTGAATTCAAA+AGG | 0.487749 | 1:-71355343 | None:intergenic |
| GATTTCTTCGACATTGTCGC+TGG | 0.493168 | 1:+71354929 | MsG0180003983.01.T01:CDS |
| AGTGTTGAAGGAAAGAGGGT+TGG | 0.493713 | 1:+71355836 | MsG0180003983.01.T01:CDS |
| CAGAGTTCGAAGTTGAAGCT+AGG | 0.494303 | 1:-71355277 | None:intergenic |
| GAGACGATTGACCAAATGCT+TGG | 0.495301 | 1:+71355598 | MsG0180003983.01.T01:CDS |
| TTGGAGTCGTTACCGTTTGG+AGG | 0.498554 | 1:+71355855 | MsG0180003983.01.T01:CDS |
| TAAAGCAGCGCCGGCTACAA+TGG | 0.502613 | 1:-71354849 | None:intergenic |
| TCCTCCACGCCGTTCACCGT+TGG | 0.502660 | 1:-71355463 | None:intergenic |
| TTTGAATTCACTTCCGTCGA+CGG | 0.509205 | 1:+71355346 | MsG0180003983.01.T01:CDS |
| AACATGCGTGACTGCGGCGG+CGG | 0.512596 | 1:-71355419 | None:intergenic |
| GGAACAACCGCCATTGTAGC+CGG | 0.514821 | 1:+71354839 | MsG0180003983.01.T01:CDS |
| ATTGTCGCTGGTACCGGCAT+TGG | 0.515883 | 1:+71354941 | MsG0180003983.01.T01:CDS |
| TTGAAGAGAATCTCCGGCAC+CGG | 0.522502 | 1:-71355099 | None:intergenic |
| CTAGCTTCAACTTCGAACTC+TGG | 0.523183 | 1:+71355278 | MsG0180003983.01.T01:CDS |
| CGTTACCGTTTGGAGGGAAA+CGG | 0.524114 | 1:+71355862 | MsG0180003983.01.T01:CDS |
| TGGAGGGAAACGGTTATTGA+CGG | 0.527447 | 1:+71355872 | MsG0180003983.01.T01:CDS |
| ACACGCATTCTCAGCATTGA+CGG | 0.529562 | 1:+71354812 | MsG0180003983.01.T01:CDS |
| CGCATTCTGTTGGAATCGTA+CGG | 0.537202 | 1:+71355624 | MsG0180003983.01.T01:CDS |
| TGTTCTTTGAAGAGAATCTC+CGG | 0.539079 | 1:-71355105 | None:intergenic |
| ACCTCTTGCTCCGCCGTAGA+CGG | 0.551267 | 1:+71355373 | MsG0180003983.01.T01:CDS |
| AGCAAGGATAGCACCAATGC+CGG | 0.552635 | 1:-71354954 | None:intergenic |
| GTTTGGTTTGGGAAGTGAAG+AGG | 0.553372 | 1:+71355812 | MsG0180003983.01.T01:CDS |
| AAGCATTTGGTCAATCGTCT+CGG | 0.559192 | 1:-71355596 | None:intergenic |
| CGGTTATTGACGGAGACTAA+CGG | 0.566958 | 1:+71355882 | MsG0180003983.01.T01:CDS |
| CGAATGGAGCTGAACTCTTG+AGG | 0.567430 | 1:-71355225 | None:intergenic |
| GATTCTCTGGCTGTGTACAT+TGG | 0.567766 | 1:-71355010 | None:intergenic |
| ACCGTCTACGGCGGAGCAAG+AGG | 0.568932 | 1:-71355374 | None:intergenic |
| TGCGTGACTGCGGCGGCGGT+AGG | 0.569292 | 1:-71355415 | None:intergenic |
| TGGAGTCGTTACCGTTTGGA+GGG | 0.569499 | 1:+71355856 | MsG0180003983.01.T01:CDS |
| AAATCAACGACGTGAGCGTG+AGG | 0.572164 | 1:-71354911 | None:intergenic |
| GACGGATCTGATCTTCGAGA+TGG | 0.576098 | 1:-71354874 | None:intergenic |
| AACGACGTGAGCGTGAGGAT+CGG | 0.580123 | 1:-71354906 | None:intergenic |
| TCCACCGTTCAGTCCTTGCA+AGG | 0.582711 | 1:+71355956 | MsG0180003983.01.T01:CDS |
| CAACCGGAGTATTTCGTCGC+CGG | 0.582946 | 1:+71355080 | MsG0180003983.01.T01:CDS |
| CATTGGTCTGCCGAATGCAT+CGG | 0.592915 | 1:-71354993 | None:intergenic |
| ATTCTCAGCATTGACGGCGG+AGG | 0.596923 | 1:+71354818 | MsG0180003983.01.T01:CDS |
| GAATCCTTGCAAGGACTGAA+CGG | 0.599507 | 1:-71355960 | None:intergenic |
| AGCAGCGCCGGCTACAATGG+CGG | 0.599958 | 1:-71354846 | None:intergenic |
| AATAACCGTTTCCCTCCAAA+CGG | 0.603874 | 1:-71355867 | None:intergenic |
| TCAAAGCAAGGTATAAGCAA+TGG | 0.604770 | 1:-71355202 | None:intergenic |
| GCTAGGAGATTCCGTTGCGT+CGG | 0.605268 | 1:-71355260 | None:intergenic |
| ATGGTTAGGTGTTGCTGACG+TGG | 0.605531 | 1:-71355314 | None:intergenic |
| GAGATTCTCTTCAAAGAACA+TGG | 0.612147 | 1:+71355108 | MsG0180003983.01.T01:CDS |
| GACGTGAGCGTGAGGATCGG+AGG | 0.616538 | 1:-71354903 | None:intergenic |
| TCTTGCTCCGCCGTAGACGG+TGG | 0.618695 | 1:+71355376 | MsG0180003983.01.T01:CDS |
| GTAACAGCTGAATCCTTGCA+AGG | 0.619384 | 1:-71355969 | None:intergenic |
| TCCTTGCAAGGACTGAACGG+TGG | 0.622124 | 1:-71355957 | None:intergenic |
| GAGATGGATTAAAGCAGCGC+CGG | 0.632030 | 1:-71354858 | None:intergenic |
| TCTGCCGAATGCATCGGCTG+CGG | 0.632893 | 1:-71354987 | None:intergenic |
| AAGAACATGCGTGACTGCGG+CGG | 0.648230 | 1:-71355422 | None:intergenic |
| TTAACGGCGTTGAGCACGTG+CGG | 0.648903 | 1:-71355546 | None:intergenic |
| GAGGATCGGAGGTTTGGAGA+CGG | 0.648909 | 1:-71354892 | None:intergenic |
| CTGCGGTGATCATTGCAGCA+AGG | 0.658132 | 1:-71354970 | None:intergenic |
| TAGGTGTTGCTGACGTGGCG+CGG | 0.660160 | 1:-71355309 | None:intergenic |
| GACTAAACCACCGTCTACGG+CGG | 0.660165 | 1:-71355383 | None:intergenic |
| TCCAACGGTGAACGGCGTGG+AGG | 0.667115 | 1:+71355462 | MsG0180003983.01.T01:CDS |
| GTGAAGAACATGCGTGACTG+CGG | 0.680632 | 1:-71355425 | None:intergenic |
| GCACCGGCGACGAAATACTC+CGG | 0.681135 | 1:-71355083 | None:intergenic |
| CGCATTCTCAGCATTGACGG+CGG | 0.683765 | 1:+71354815 | MsG0180003983.01.T01:CDS |
| GAACTCTTGAGGTCAAAGCA+AGG | 0.690315 | 1:-71355214 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAATTCAAAAGGTTTGAAA+TGG | - | Chr1:71355336-71355355 | None:intergenic | 25.0% |
| !! | TTTTTCTGTGATAATACGAA+CGG | - | Chr1:71355035-71355054 | None:intergenic | 25.0% |
| AGAACAATGTCAACCATTAA+CGG | - | Chr1:71355565-71355584 | None:intergenic | 30.0% | |
| ATACAGTTACCGTTACTTAA+CGG | + | Chr1:71355770-71355789 | MsG0180003983.01.T01:intron | 30.0% | |
| TCGTTAGAAAAGAAGATTCA+CGG | + | Chr1:71355152-71355171 | MsG0180003983.01.T01:CDS | 30.0% | |
| ! | TCAAAAGGTTTGAAATGGTT+AGG | - | Chr1:71355331-71355350 | None:intergenic | 30.0% |
| !!! | GATCTTTTGGTTCTTTCATT+GGG | + | Chr1:71355484-71355503 | MsG0180003983.01.T01:CDS | 30.0% |
| AAACAAACGCCGTTAAGTAA+CGG | - | Chr1:71355782-71355801 | None:intergenic | 35.0% | |
| ATGGTTGACATTGTTCTTGA+CGG | + | Chr1:71355568-71355587 | MsG0180003983.01.T01:CDS | 35.0% | |
| GAGATTCTCTTCAAAGAACA+TGG | + | Chr1:71355108-71355127 | MsG0180003983.01.T01:CDS | 35.0% | |
| TCAAAGCAAGGTATAAGCAA+TGG | - | Chr1:71355205-71355224 | None:intergenic | 35.0% | |
| ! | CAACAAACGTGATTTTCCAA+CGG | + | Chr1:71355447-71355466 | MsG0180003983.01.T01:CDS | 35.0% |
| ! | TGTTCTTTGAAGAGAATCTC+CGG | - | Chr1:71355108-71355127 | None:intergenic | 35.0% |
| ! | TTGGTTCTTTCATTGGGAAA+CGG | + | Chr1:71355490-71355509 | MsG0180003983.01.T01:CDS | 35.0% |
| !! | TTCACGGTTGTTGACTTTAA+AGG | + | Chr1:71355168-71355187 | MsG0180003983.01.T01:CDS | 35.0% |
| !!! | GGATCTTTTGGTTCTTTCAT+TGG | + | Chr1:71355483-71355502 | MsG0180003983.01.T01:CDS | 35.0% |
| !!! | TTTGTTTTGTAACAGGCGTT+TGG | + | Chr1:71355795-71355814 | MsG0180003983.01.T01:intron | 35.0% |
| AAGCATTTGGTCAATCGTCT+CGG | - | Chr1:71355599-71355618 | None:intergenic | 40.0% | |
| AATAACCGTTTCCCTCCAAA+CGG | - | Chr1:71355870-71355889 | None:intergenic | 40.0% | |
| AGAATGCGTTTCCAAGCATT+TGG | - | Chr1:71355612-71355631 | None:intergenic | 40.0% | |
| CTCTACAAACGCAAATCAAC+CGG | + | Chr1:71355064-71355083 | MsG0180003983.01.T01:CDS | 40.0% | |
| GAGACTAACGGAAACAGAAT+CGG | + | Chr1:71355894-71355913 | MsG0180003983.01.T01:CDS | 40.0% | |
| TCGACGGAAGTGAATTCAAA+AGG | - | Chr1:71355346-71355365 | None:intergenic | 40.0% | |
| TCTCCAAACTCGAACACAAA+TGG | + | Chr1:71354756-71354775 | MsG0180003983.01.T01:CDS | 40.0% | |
| TTTGAATTCACTTCCGTCGA+CGG | + | Chr1:71355346-71355365 | MsG0180003983.01.T01:CDS | 40.0% | |
| TTTGTAACAGGCGTTTGGTT+TGG | + | Chr1:71355800-71355819 | MsG0180003983.01.T01:intron | 40.0% | |
| ! | AAGCCATTTGTGTTCGAGTT+TGG | - | Chr1:71354762-71354781 | None:intergenic | 40.0% |
| ! | AATGCTGAGAATGCGTGTTT+TGG | - | Chr1:71354810-71354829 | None:intergenic | 40.0% |
| ! | GATAATACGAACGGATTCTC+TGG | - | Chr1:71355026-71355045 | None:intergenic | 40.0% |
| ! | TACGGATTACGTCAGAATTC+AGG | + | Chr1:71355642-71355661 | MsG0180003983.01.T01:CDS | 40.0% |
| ! | TTGTAACAGGCGTTTGGTTT+GGG | + | Chr1:71355801-71355820 | MsG0180003983.01.T01:intron | 40.0% |
| !! | AAGTGAAGAGGAAGTGTTGA+AGG | + | Chr1:71355824-71355843 | MsG0180003983.01.T01:CDS | 40.0% |
| !! | AGGAAGTGTTGAAGGAAAGA+GGG | + | Chr1:71355832-71355851 | MsG0180003983.01.T01:CDS | 40.0% |
| !!! | AACGGCGTTTGTTTTGTAAC+AGG | + | Chr1:71355788-71355807 | MsG0180003983.01.T01:intron | 40.0% |
| CAGAGTTCGAAGTTGAAGCT+AGG | - | Chr1:71355280-71355299 | None:intergenic | 45.0% | |
| CATGACTAAACCACCGTCTA+CGG | - | Chr1:71355389-71355408 | None:intergenic | 45.0% | |
| CGCATTCTGTTGGAATCGTA+CGG | + | Chr1:71355624-71355643 | MsG0180003983.01.T01:CDS | 45.0% | |
| CGGTTATTGACGGAGACTAA+CGG | + | Chr1:71355882-71355901 | MsG0180003983.01.T01:CDS | 45.0% | |
| CTAGCTTCAACTTCGAACTC+TGG | + | Chr1:71355278-71355297 | MsG0180003983.01.T01:CDS | 45.0% | |
| GAACTCTTGAGGTCAAAGCA+AGG | - | Chr1:71355217-71355236 | None:intergenic | 45.0% | |
| GAATCCTTGCAAGGACTGAA+CGG | - | Chr1:71355963-71355982 | None:intergenic | 45.0% | |
| GAGACGATTGACCAAATGCT+TGG | + | Chr1:71355598-71355617 | MsG0180003983.01.T01:CDS | 45.0% | |
| GATTCTCTGGCTGTGTACAT+TGG | - | Chr1:71355013-71355032 | None:intergenic | 45.0% | |
| GATTTCTTCGACATTGTCGC+TGG | + | Chr1:71354929-71354948 | MsG0180003983.01.T01:CDS | 45.0% | |
| GCTGTTACATCTCTCGCTAA+TGG | + | Chr1:71355984-71356003 | MsG0180003983.01.T01:CDS | 45.0% | |
| GTAACAGCTGAATCCTTGCA+AGG | - | Chr1:71355972-71355991 | None:intergenic | 45.0% | |
| TGCTTGGAAACGCATTCTGT+TGG | + | Chr1:71355614-71355633 | MsG0180003983.01.T01:CDS | 45.0% | |
| TGGAGGGAAACGGTTATTGA+CGG | + | Chr1:71355872-71355891 | MsG0180003983.01.T01:CDS | 45.0% | |
| TTGAATTCACTTCCGTCGAC+GGG | + | Chr1:71355347-71355366 | MsG0180003983.01.T01:CDS | 45.0% | |
| ! | ACACGCATTCTCAGCATTGA+CGG | + | Chr1:71354812-71354831 | MsG0180003983.01.T01:CDS | 45.0% |
| ! | AGTGTTGAAGGAAAGAGGGT+TGG | + | Chr1:71355836-71355855 | MsG0180003983.01.T01:CDS | 45.0% |
| ! | CGTGATTTTCCAACGGTGAA+CGG | + | Chr1:71355454-71355473 | MsG0180003983.01.T01:CDS | 45.0% |
| ! | GTGCAACGTCTTGTTGCTAA+TGG | + | Chr1:71355924-71355943 | MsG0180003983.01.T01:CDS | 45.0% |
| ! | TCGGCTCGTGAAAAAACGAA+TGG | - | Chr1:71355244-71355263 | None:intergenic | 45.0% |
| !! | GAGGAAGTGTTGAAGGAAAG+AGG | + | Chr1:71355831-71355850 | MsG0180003983.01.T01:CDS | 45.0% |
| !! | GTTTGGTTTGGGAAGTGAAG+AGG | + | Chr1:71355812-71355831 | MsG0180003983.01.T01:CDS | 45.0% |
| AAATCAACGACGTGAGCGTG+AGG | - | Chr1:71354914-71354933 | None:intergenic | 50.0% | |
| AGCAAGGATAGCACCAATGC+CGG | - | Chr1:71354957-71354976 | None:intergenic | 50.0% | |
| CGAATGGAGCTGAACTCTTG+AGG | - | Chr1:71355228-71355247 | None:intergenic | 50.0% | |
| CGTTACCGTTTGGAGGGAAA+CGG | + | Chr1:71355862-71355881 | MsG0180003983.01.T01:CDS | 50.0% | |
| GACGGATCTGATCTTCGAGA+TGG | - | Chr1:71354877-71354896 | None:intergenic | 50.0% | |
| GTGAAGAACATGCGTGACTG+CGG | - | Chr1:71355428-71355447 | None:intergenic | 50.0% | |
| TGGAGTCGTTACCGTTTGGA+GGG | + | Chr1:71355856-71355875 | MsG0180003983.01.T01:CDS | 50.0% | |
| TTCGACATTGTCGCTGGTAC+CGG | + | Chr1:71354935-71354954 | MsG0180003983.01.T01:CDS | 50.0% | |
| TTGAAGAGAATCTCCGGCAC+CGG | - | Chr1:71355102-71355121 | None:intergenic | 50.0% | |
| TTGGAGTCGTTACCGTTTGG+AGG | + | Chr1:71355855-71355874 | MsG0180003983.01.T01:CDS | 50.0% | |
| ! | CATTGGTCTGCCGAATGCAT+CGG | - | Chr1:71354996-71355015 | None:intergenic | 50.0% |
| ! | TTTTTCACGAGCCGACGCAA+CGG | + | Chr1:71355249-71355268 | MsG0180003983.01.T01:CDS | 50.0% |
| !! | ATGGTTAGGTGTTGCTGACG+TGG | - | Chr1:71355317-71355336 | None:intergenic | 50.0% |
| !! | GAGATGGATTAAAGCAGCGC+CGG | - | Chr1:71354861-71354880 | None:intergenic | 50.0% |
| AACGACGTGAGCGTGAGGAT+CGG | - | Chr1:71354909-71354928 | None:intergenic | 55.0% | |
| AAGAACATGCGTGACTGCGG+CGG | - | Chr1:71355425-71355444 | None:intergenic | 55.0% | |
| ATCACCGCAGCCGATGCATT+CGG | + | Chr1:71354983-71355002 | MsG0180003983.01.T01:CDS | 55.0% | |
| CACGTGCTCAACGCCGTTAA+TGG | + | Chr1:71355549-71355568 | MsG0180003983.01.T01:CDS | 55.0% | |
| CTGCGGTGATCATTGCAGCA+AGG | - | Chr1:71354973-71354992 | None:intergenic | 55.0% | |
| GACTAAACCACCGTCTACGG+CGG | - | Chr1:71355386-71355405 | None:intergenic | 55.0% | |
| GAGGATCGGAGGTTTGGAGA+CGG | - | Chr1:71354895-71354914 | None:intergenic | 55.0% | |
| GCTAGGAGATTCCGTTGCGT+CGG | - | Chr1:71355263-71355282 | None:intergenic | 55.0% | |
| GGAACAACCGCCATTGTAGC+CGG | + | Chr1:71354839-71354858 | MsG0180003983.01.T01:CDS | 55.0% | |
| GGGTTGGAGTCGTTACCGTT+TGG | + | Chr1:71355852-71355871 | MsG0180003983.01.T01:CDS | 55.0% | |
| TCCACCGTTCAGTCCTTGCA+AGG | + | Chr1:71355956-71355975 | MsG0180003983.01.T01:CDS | 55.0% | |
| TCCTTGCAAGGACTGAACGG+TGG | - | Chr1:71355960-71355979 | None:intergenic | 55.0% | |
| ! | CAACCGGAGTATTTCGTCGC+CGG | + | Chr1:71355080-71355099 | MsG0180003983.01.T01:CDS | 55.0% |
| ! | CAAGGACTGAACGGTGGACT+TGG | - | Chr1:71355954-71355973 | None:intergenic | 55.0% |
| ! | GAACGGCGTGGAGGATCTTT+TGG | + | Chr1:71355471-71355490 | MsG0180003983.01.T01:CDS | 55.0% |
| ! | GCAAGAGGTTTTCCCGTCGA+CGG | - | Chr1:71355362-71355381 | None:intergenic | 55.0% |
| ! | TTAACGGCGTTGAGCACGTG+CGG | - | Chr1:71355549-71355568 | None:intergenic | 55.0% |
| ! | TTTTCCAACGGTGAACGGCG+TGG | + | Chr1:71355459-71355478 | MsG0180003983.01.T01:CDS | 55.0% |
| !! | ATTCTCAGCATTGACGGCGG+AGG | + | Chr1:71354818-71354837 | MsG0180003983.01.T01:CDS | 55.0% |
| !! | ATTGTCGCTGGTACCGGCAT+TGG | + | Chr1:71354941-71354960 | MsG0180003983.01.T01:CDS | 55.0% |
| !! | CGCATTCTCAGCATTGACGG+CGG | + | Chr1:71354815-71354834 | MsG0180003983.01.T01:CDS | 55.0% |
| !! | TAAAGCAGCGCCGGCTACAA+TGG | - | Chr1:71354852-71354871 | None:intergenic | 55.0% |
| ACCTCTTGCTCCGCCGTAGA+CGG | + | Chr1:71355373-71355392 | MsG0180003983.01.T01:CDS | 60.0% | |
| GAGCGTGAGGATCGGAGGTT+TGG | - | Chr1:71354901-71354920 | None:intergenic | 60.0% | |
| GAGTATTTCGTCGCCGGTGC+CGG | + | Chr1:71355086-71355105 | MsG0180003983.01.T01:CDS | 60.0% | |
| ! | GCACCGGCGACGAAATACTC+CGG | - | Chr1:71355086-71355105 | None:intergenic | 60.0% |
| !! | TAGGTGTTGCTGACGTGGCG+CGG | - | Chr1:71355312-71355331 | None:intergenic | 60.0% |
| !! | TCTGCCGAATGCATCGGCTG+CGG | - | Chr1:71354990-71355009 | None:intergenic | 60.0% |
| AACATGCGTGACTGCGGCGG+CGG | - | Chr1:71355422-71355441 | None:intergenic | 65.0% | |
| ACCGTCTACGGCGGAGCAAG+AGG | - | Chr1:71355377-71355396 | None:intergenic | 65.0% | |
| GACGTGAGCGTGAGGATCGG+AGG | - | Chr1:71354906-71354925 | None:intergenic | 65.0% | |
| TCCAACGGTGAACGGCGTGG+AGG | + | Chr1:71355462-71355481 | MsG0180003983.01.T01:CDS | 65.0% | |
| TCCTCCACGCCGTTCACCGT+TGG | - | Chr1:71355466-71355485 | None:intergenic | 65.0% | |
| TCTTGCTCCGCCGTAGACGG+TGG | + | Chr1:71355376-71355395 | MsG0180003983.01.T01:CDS | 65.0% | |
| !! | AGCAGCGCCGGCTACAATGG+CGG | - | Chr1:71354849-71354868 | None:intergenic | 65.0% |
| TGCGTGACTGCGGCGGCGGT+AGG | - | Chr1:71355418-71355437 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 71354713 | 71356013 | 71354713 | ID=MsG0180003983.01;Name=MsG0180003983.01 |
| Chr1 | mRNA | 71354713 | 71356013 | 71354713 | ID=MsG0180003983.01.T01;Parent=MsG0180003983.01;Name=MsG0180003983.01.T01;_AED=0.19;_eAED=0.20;_QI=0|0|0|1|1|1|2|0|384 |
| Chr1 | exon | 71354713 | 71355663 | 71354713 | ID=MsG0180003983.01.T01:exon:30780;Parent=MsG0180003983.01.T01 |
| Chr1 | exon | 71355810 | 71356013 | 71355810 | ID=MsG0180003983.01.T01:exon:30781;Parent=MsG0180003983.01.T01 |
| Chr1 | CDS | 71354713 | 71355663 | 71354713 | ID=MsG0180003983.01.T01:cds;Parent=MsG0180003983.01.T01 |
| Chr1 | CDS | 71355810 | 71356013 | 71355810 | ID=MsG0180003983.01.T01:cds;Parent=MsG0180003983.01.T01 |
| Gene Sequence |
| Protein sequence |
