AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023426.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023426.01.T01 MTR_7g059070 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_5g029820 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_5g029770 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_4g065990 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_4g088150 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_2g035230 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_8g092720 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_8g063500 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_8g103245 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_8g092820 86.614 127 17 0 1 127 1 127 7.51e-75 219
MsG0480023426.01.T01 MTR_7g013610 86.614 127 17 0 1 127 100 226 1.08e-73 220
MsG0480023426.01.T01 MTR_4g097175 83.465 127 21 0 1 127 1 127 4.48e-72 213
MsG0480023426.01.T01 MTR_1g023630 83.465 127 21 0 1 127 1 127 4.48e-72 213
MsG0480023426.01.T01 MTR_8g061940 83.465 127 21 0 1 127 1 127 4.48e-72 213
MsG0480023426.01.T01 MTR_8g099430 82.677 127 22 0 1 127 1 127 1.36e-71 211
MsG0480023426.01.T01 MTR_4g097170 82.677 127 22 0 1 127 42 168 3.07e-70 209
MsG0480023426.01.T01 MTR_2g082310 78.571 126 20 1 1 119 1 126 1.24e-66 199
MsG0480023426.01.T01 MTR_2g082340 78.571 126 20 1 1 119 1 126 1.24e-66 199
MsG0480023426.01.T01 MTR_2g082370 72.263 137 28 1 1 127 1 137 2.32e-61 186
MsG0480023426.01.T01 MTR_2g082400 71.094 128 35 2 1 127 1 127 1.04e-55 171
MsG0480023426.01.T01 MTR_1g023630 95.122 82 4 0 1 82 1 82 9.46e-52 160
MsG0480023426.01.T01 MTR_7g013600 72.816 103 28 0 25 127 2 104 1.66e-48 152
MsG0480023426.01.T01 MTR_0197s0100 63.235 136 50 0 1 136 1 136 4.60e-46 147
MsG0480023426.01.T01 MTR_5g024630 63.964 111 37 2 1 111 1 108 3.89e-38 125
MsG0480023426.01.T01 MTR_7g023830 54.331 127 57 1 1 127 1 126 3.56e-37 124
MsG0480023426.01.T01 MTR_5g024530 54.128 109 27 3 19 127 68 153 2.39e-26 97.4
MsG0480023426.01.T01 MTR_7g023880 46.825 126 53 3 3 127 18 130 3.57e-26 96.7
MsG0480023426.01.T01 MTR_8g027840 53.521 71 32 1 37 106 28 98 6.28e-16 69.7
MsG0480023426.01.T01 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.75e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023426.01.T01 AT5G65360 85.827 127 18 0 1 127 1 127 2.51e-74 218
MsG0480023426.01.T01 AT5G10390 85.827 127 18 0 1 127 1 127 2.51e-74 218
MsG0480023426.01.T01 AT5G10400 85.827 127 18 0 1 127 1 127 2.51e-74 218
MsG0480023426.01.T01 AT3G27360 85.827 127 18 0 1 127 1 127 2.51e-74 218
MsG0480023426.01.T01 AT1G09200 85.827 127 18 0 1 127 1 127 2.51e-74 218
MsG0480023426.01.T01 AT5G10980 83.465 127 21 0 1 127 1 127 4.69e-72 213
MsG0480023426.01.T01 AT4G40040 83.465 127 21 0 1 127 1 127 4.69e-72 213
MsG0480023426.01.T01 AT4G40040 83.465 127 21 0 1 127 1 127 4.69e-72 213
MsG0480023426.01.T01 AT4G40040 83.465 127 21 0 1 127 1 127 4.69e-72 213
MsG0480023426.01.T01 AT4G40030 83.465 127 21 0 1 127 1 127 4.69e-72 213
MsG0480023426.01.T01 AT4G40030 83.465 127 21 0 1 127 1 127 4.69e-72 213
MsG0480023426.01.T01 AT4G40030 83.465 127 21 0 1 127 1 127 4.69e-72 213
MsG0480023426.01.T01 AT4G40030 83.465 127 21 0 1 127 29 155 1.19e-71 213
MsG0480023426.01.T01 AT5G65350 81.890 127 23 0 1 127 1 127 1.09e-70 209
MsG0480023426.01.T01 AT1G75600 80.315 127 25 0 1 127 1 127 6.95e-69 204
MsG0480023426.01.T01 AT1G13370 81.102 127 24 0 1 127 1 127 1.20e-68 204
MsG0480023426.01.T01 AT1G19890 77.953 127 20 2 1 119 1 127 1.07e-62 189
MsG0480023426.01.T01 AT5G12910 59.843 127 46 2 1 127 1 122 9.06e-48 151
MsG0480023426.01.T01 AT1G01370 41.026 117 66 2 24 138 63 178 3.47e-17 74.3
MsG0480023426.01.T01 AT1G01370 41.026 117 66 2 24 138 63 178 3.47e-17 74.3
MsG0480023426.01.T01 AT1G01370 41.026 117 66 2 24 138 63 178 3.47e-17 74.3
MsG0480023426.01.T01 AT1G01370 41.026 117 66 2 24 138 63 178 3.47e-17 74.3

Find 38 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGTGGCGAGCTGCTTC+CGG 0.229871 4:+85070513 None:intergenic
CTTTGTGGCGAGCTGCTTCC+GGG 0.231058 4:+85070514 None:intergenic
CAGTGCTGGTTCTGCTCTTC+AGG 0.253307 4:-85070303 MsG0480023426.01.T01:CDS
AGGGTTACGAAATTGAAAAT+TGG 0.263711 4:+85070138 None:intergenic
TCTTGCAAGTTGAATGTCCT+TGG 0.320782 4:+85070200 None:intergenic
TCTCTCAAAGCAACTGTTCC+AGG 0.333816 4:+85070434 None:intergenic
GGGTTACGAAATTGAAAATT+GGG 0.339758 4:+85070139 None:intergenic
CTTGCAAGTTGAATGTCCTT+GGG 0.355623 4:+85070201 None:intergenic
GGAAGCAGCTGAAGCTTACT+TGG 0.369838 4:-85070282 MsG0480023426.01.T01:CDS
TGATTTCCGAGCGGCCTTTG+TGG 0.424857 4:+85070499 None:intergenic
GAGCAGAACCAGCACTGCTC+TGG 0.425796 4:+85070309 None:intergenic
TTCTTCACTCCTCCGGTCGC+CGG 0.442119 4:+85070473 None:intergenic
CTTAGATTCCAGAGCAGTGC+TGG 0.448605 4:-85070317 MsG0480023426.01.T01:CDS
GACGTTGGAATGGAAGTTTA+CGG 0.458530 4:+85070375 None:intergenic
CGGAAATCAGCTCCGGCGAC+CGG 0.461897 4:-85070485 MsG0480023426.01.T01:CDS
GCAGCTGAAGCTTACTTGGT+TGG 0.472209 4:-85070278 MsG0480023426.01.T01:CDS
CCACCGGCGGCAAGGCTCCC+CGG 0.480154 4:-85070532 MsG0480023426.01.T01:CDS
AGCTCGCCACAAAGGCCGCT+CGG 0.491073 4:-85070505 MsG0480023426.01.T01:CDS
GGCCGCTCGGAAATCAGCTC+CGG 0.498921 4:-85070492 MsG0480023426.01.T01:CDS
GTGTGGCTTCTTCACTCCTC+CGG 0.499925 4:+85070466 None:intergenic
CTTCCGGGGAGCCTTGCCGC+CGG 0.507682 4:+85070529 None:intergenic
GGACTTGCGAGCTGTTTGCT+TGG 0.512299 4:+85070553 None:intergenic
TCGCAAGTCCACCGGCGGCA+AGG 0.530180 4:-85070540 MsG0480023426.01.T01:CDS
CCGGGGAGCCTTGCCGCCGG+TGG 0.548377 4:+85070532 None:intergenic
CAAACAGCTCGCAAGTCCAC+CGG 0.573307 4:-85070548 MsG0480023426.01.T01:CDS
TTTGTGGCGAGCTGCTTCCG+GGG 0.576886 4:+85070515 None:intergenic
AAGAAGAATCAGAGGCGAGA+GGG 0.597241 4:-85070181 MsG0480023426.01.T01:CDS
TCACGAACAAGACGTTGGAA+TGG 0.598115 4:+85070365 None:intergenic
AAATCAGCTCCGGCGACCGG+AGG 0.599225 4:-85070482 MsG0480023426.01.T01:CDS
CCGGAAGCAGCTCGCCACAA+AGG 0.611340 4:-85070513 MsG0480023426.01.T01:CDS
CGATTTCACGAACAAGACGT+TGG 0.613448 4:+85070360 None:intergenic
GAGAGTTACAATCATGCCCA+AGG 0.637894 4:-85070217 MsG0480023426.01.T01:CDS
CAAGAAGAATCAGAGGCGAG+AGG 0.648850 4:-85070182 MsG0480023426.01.T01:CDS
ACAGCTCGCAAGTCCACCGG+CGG 0.655092 4:-85070545 MsG0480023426.01.T01:CDS
CAACTTGCAAGAAGAATCAG+AGG 0.655500 4:-85070189 MsG0480023426.01.T01:CDS
AAGCCACACAGATTCAGACC+TGG 0.672238 4:-85070452 MsG0480023426.01.T01:CDS
GTTCCAGGTCTGAATCTGTG+TGG 0.688220 4:+85070449 None:intergenic
CGCCGGAGCTGATTTCCGAG+CGG 0.728257 4:+85070490 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CAACTTGCAAGAAGAATCAG+AGG - Chr4:85070516-85070535 MsG0480023426.01.T01:CDS 40.0%
GACGTTGGAATGGAAGTTTA+CGG + Chr4:85070333-85070352 None:intergenic 40.0%
! CTTGCAAGTTGAATGTCCTT+GGG + Chr4:85070507-85070526 None:intergenic 40.0%
! TCTTGCAAGTTGAATGTCCT+TGG + Chr4:85070508-85070527 None:intergenic 40.0%
AAGAAGAATCAGAGGCGAGA+GGG - Chr4:85070524-85070543 MsG0480023426.01.T01:CDS 45.0%
CGATTTCACGAACAAGACGT+TGG + Chr4:85070348-85070367 None:intergenic 45.0%
GAGAGTTACAATCATGCCCA+AGG - Chr4:85070488-85070507 MsG0480023426.01.T01:CDS 45.0%
TCACGAACAAGACGTTGGAA+TGG + Chr4:85070343-85070362 None:intergenic 45.0%
TCTCTCAAAGCAACTGTTCC+AGG + Chr4:85070274-85070293 None:intergenic 45.0%
AAGCCACACAGATTCAGACC+TGG - Chr4:85070253-85070272 MsG0480023426.01.T01:CDS 50.0%
CAAGAAGAATCAGAGGCGAG+AGG - Chr4:85070523-85070542 MsG0480023426.01.T01:CDS 50.0%
CTTAGATTCCAGAGCAGTGC+TGG - Chr4:85070388-85070407 MsG0480023426.01.T01:CDS 50.0%
GCAGCTGAAGCTTACTTGGT+TGG - Chr4:85070427-85070446 MsG0480023426.01.T01:CDS 50.0%
GGAAGCAGCTGAAGCTTACT+TGG - Chr4:85070423-85070442 MsG0480023426.01.T01:CDS 50.0%
GTTCCAGGTCTGAATCTGTG+TGG + Chr4:85070259-85070278 None:intergenic 50.0%
CAAACAGCTCGCAAGTCCAC+CGG - Chr4:85070157-85070176 MsG0480023426.01.T01:CDS 55.0%
GGACTTGCGAGCTGTTTGCT+TGG + Chr4:85070155-85070174 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + Chr4:85070242-85070261 None:intergenic 55.0%
TGATTTCCGAGCGGCCTTTG+TGG + Chr4:85070209-85070228 None:intergenic 55.0%
!! CAGTGCTGGTTCTGCTCTTC+AGG - Chr4:85070402-85070421 MsG0480023426.01.T01:CDS 55.0%
CCTTTGTGGCGAGCTGCTTC+CGG + Chr4:85070195-85070214 None:intergenic 60.0%
CTTTGTGGCGAGCTGCTTCC+GGG + Chr4:85070194-85070213 None:intergenic 60.0%
GAGCAGAACCAGCACTGCTC+TGG + Chr4:85070399-85070418 None:intergenic 60.0%
TTCTTCACTCCTCCGGTCGC+CGG + Chr4:85070235-85070254 None:intergenic 60.0%
TTTGTGGCGAGCTGCTTCCG+GGG + Chr4:85070193-85070212 None:intergenic 60.0%
AAATCAGCTCCGGCGACCGG+AGG - Chr4:85070223-85070242 MsG0480023426.01.T01:CDS 65.0%
ACAGCTCGCAAGTCCACCGG+CGG - Chr4:85070160-85070179 MsG0480023426.01.T01:CDS 65.0%
AGCTCGCCACAAAGGCCGCT+CGG - Chr4:85070200-85070219 MsG0480023426.01.T01:CDS 65.0%
CCGGAAGCAGCTCGCCACAA+AGG - Chr4:85070192-85070211 MsG0480023426.01.T01:CDS 65.0%
CGGAAATCAGCTCCGGCGAC+CGG - Chr4:85070220-85070239 MsG0480023426.01.T01:CDS 65.0%
GGCCGCTCGGAAATCAGCTC+CGG - Chr4:85070213-85070232 MsG0480023426.01.T01:CDS 65.0%
! CGCCGGAGCTGATTTCCGAG+CGG + Chr4:85070218-85070237 None:intergenic 65.0%
TCGCAAGTCCACCGGCGGCA+AGG - Chr4:85070165-85070184 MsG0480023426.01.T01:CDS 70.0%
CTTCCGGGGAGCCTTGCCGC+CGG + Chr4:85070179-85070198 None:intergenic 75.0%
! CCACCGGCGGCAAGGCTCCC+CGG - Chr4:85070173-85070192 MsG0480023426.01.T01:CDS 80.0%
! CCGGGGAGCCTTGCCGCCGG+TGG + Chr4:85070176-85070195 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
Chr4 gene 85070142 85070585 85070142 ID=MsG0480023426.01;Name=MsG0480023426.01
Chr4 mRNA 85070142 85070585 85070142 ID=MsG0480023426.01.T01;Parent=MsG0480023426.01;Name=MsG0480023426.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|147
Chr4 exon 85070142 85070585 85070142 ID=MsG0480023426.01.T01:exon:2816;Parent=MsG0480023426.01.T01
Chr4 CDS 85070142 85070585 85070142 ID=MsG0480023426.01.T01:cds;Parent=MsG0480023426.01.T01
Gene Sequence

>MsG0480023426.01.T01

ATGGCACGTACCAAGCAAACAGCTCGCAAGTCCACCGGCGGCAAGGCTCCCCGGAAGCAGCTCGCCACAAAGGCCGCTCGGAAATCAGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACAGATTCAGACCTGGAACAGTTGCTTTGAGAGAGATTCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCCGTAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAAGATTTCAAGACAGATCTTAGATTCCAGAGCAGTGCTGGTTCTGCTCTTCAGGAAGCAGCTGAAGCTTACTTGGTTGGACTCTTTGAAGACACAAACTTTGTGCAATTCATGCTAAGAGAGTTACAATCATGCCCAAGGACATTCAACTTGCAAGAAGAATCAGAGGCGAGAGGGCTTAGTTTCAATTTCAATTCCCAATTTTCAATTTCGTAA

Protein sequence

>MsG0480023426.01.T01

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAGSALQEAAEAYLVGLFEDTNFVQFMLRELQSCPRTFNLQEESEARGLSFNFNSQFSIS*