AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016027.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016027.01.T01 MTR_3g082070 91.713 181 9 2 1 181 1 175 2.59e-120 337
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016027.01.T01 AT4G15140 75.472 53 12 1 20 71 19 71 8.70e-20 82.0

Find 45 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCCTTCAATCTCAAATT+TGG 0.164475 3:-78117249 MsG0380016027.01.T01:CDS
ACTACTCTCAAAGCACTATT+TGG 0.262045 3:-78117410 MsG0380016027.01.T01:CDS
ACCCTATTCTCCCATGGATT+TGG 0.270878 3:-78117370 MsG0380016027.01.T01:CDS
AAATCATAAGCAGAGTTATC+TGG 0.289654 3:+78117435 None:intergenic
TAACCCCATCATCAATAATA+AGG 0.309837 3:-78117148 MsG0380016027.01.T01:intron
ATGTCATAGGAGAGTTTCTT+TGG 0.315934 3:+78117336 None:intergenic
ATTTGATCCTTGAAGGTGTT+GGG 0.350471 3:+78117178 None:intergenic
AAGCACTATTTGGTCCCCTT+TGG 0.370267 3:-78117400 MsG0380016027.01.T01:CDS
AAATACCTTATTATTGATGA+TGG 0.374171 3:+78117143 None:intergenic
ATTTCTCCCCAACACCTTCA+AGG 0.390688 3:-78117185 MsG0380016027.01.T01:CDS
TCCAAATCCATGGGAGAATA+GGG 0.407407 3:+78117369 None:intergenic
CATGCACATTTGATCCTTGA+AGG 0.408025 3:+78117171 None:intergenic
GAAACCAAATTTGAGATTGA+AGG 0.423550 3:+78117245 None:intergenic
ACAAGGTTGGTTCTAAGCTT+AGG 0.427431 3:-78117287 MsG0380016027.01.T01:CDS
ATCCAAATCCATGGGAGAAT+AGG 0.427751 3:+78117368 None:intergenic
GGTAGCATCACATGAGCAAT+GGG 0.433096 3:+78116825 None:intergenic
AATACCTTATTATTGATGAT+GGG 0.459464 3:+78117144 None:intergenic
CATTTGATCCTTGAAGGTGT+TGG 0.463929 3:+78117177 None:intergenic
CTACCAATGTCTTTCAAAGA+TGG 0.465367 3:-78116807 MsG0380016027.01.T01:CDS
AGCTCCTAAGCCAATGTCAT+AGG 0.476044 3:+78117323 None:intergenic
TCCATGGGAGAATAGGGTCT+TGG 0.487632 3:+78117375 None:intergenic
AATCATAAGCAGAGTTATCT+GGG 0.497222 3:+78117436 None:intergenic
AATAGGGTCTTGGAACCAAA+GGG 0.508609 3:+78117385 None:intergenic
CTCTCCTATGACATTGGCTT+AGG 0.509647 3:-78117327 MsG0380016027.01.T01:CDS
AAGAAACTCTCCTATGACAT+TGG 0.513119 3:-78117333 MsG0380016027.01.T01:CDS
TGTTCTTCTTCTGCTTCTTG+TGG 0.515281 3:+78117540 None:intergenic
TCTCCATCTTTGAAAGACAT+TGG 0.535368 3:+78116804 None:intergenic
ATACCTTATTATTGATGATG+GGG 0.543732 3:+78117145 None:intergenic
TGGTAGCATCACATGAGCAA+TGG 0.550773 3:+78116824 None:intergenic
TTTGTAGTGTTCAGTAACAA+TGG 0.553446 3:+78117510 None:intergenic
AGATGCAGTGTCAACAAGGT+TGG 0.554476 3:-78117300 MsG0380016027.01.T01:CDS
ATGTGATACAGAGATGGGGC+AGG 0.556775 3:-78116899 MsG0380016027.01.T01:intron
TTTGATCCTTGAAGGTGTTG+GGG 0.561529 3:+78117179 None:intergenic
TCCAAGACCCTATTCTCCCA+TGG 0.565449 3:-78117376 MsG0380016027.01.T01:CDS
AGTAATGAGATCCAAATCCA+TGG 0.566074 3:+78117359 None:intergenic
GAATAGGGTCTTGGAACCAA+AGG 0.567058 3:+78117384 None:intergenic
CTTCAAAACAGTTCAAGAGA+TGG 0.567072 3:-78116863 MsG0380016027.01.T01:CDS
AGCTAGATGCAGTGTCAACA+AGG 0.580501 3:-78117304 MsG0380016027.01.T01:CDS
AGAAGCTCTAGTGACATGAA+TGG 0.598657 3:+78117468 None:intergenic
AAAACAGTTCAAGAGATGGA+AGG 0.606838 3:-78116859 MsG0380016027.01.T01:CDS
GTAATGAGATCCAAATCCAT+GGG 0.641690 3:+78117360 None:intergenic
TGTGATACAGAGATGGGGCA+GGG 0.647718 3:-78116898 MsG0380016027.01.T01:intron
TACTATGTGATACAGAGATG+GGG 0.699067 3:-78116903 MsG0380016027.01.T01:intron
ATAGGGTCTTGGAACCAAAG+GGG 0.746078 3:+78117386 None:intergenic
GTAGCATCACATGAGCAATG+GGG 0.782689 3:+78116826 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTAAACTCTT+TGG - Chr3:78117329-78117348 MsG0380016027.01.T01:CDS 10.0%
!! AAATACCTTATTATTGATGA+TGG + Chr3:78117215-78117234 None:intergenic 20.0%
!! GATGAAATTGAAGTAATTAT+TGG - Chr3:78117412-78117431 MsG0380016027.01.T01:CDS 20.0%
!!! AATACCTTATTATTGATGAT+GGG + Chr3:78117214-78117233 None:intergenic 20.0%
! AATTCCTTCAATCTCAAATT+TGG - Chr3:78117106-78117125 MsG0380016027.01.T01:intron 25.0%
!! AATTATTGGTGATAACTTGT+GGG - Chr3:78117426-78117445 MsG0380016027.01.T01:CDS 25.0%
!! CTGTTCTGTTTTATGATATT+GGG - Chr3:78117285-78117304 MsG0380016027.01.T01:CDS 25.0%
!! TAATTATTGGTGATAACTTG+TGG - Chr3:78117425-78117444 MsG0380016027.01.T01:CDS 25.0%
!! TCTGTTCTGTTTTATGATAT+TGG - Chr3:78117284-78117303 MsG0380016027.01.T01:CDS 25.0%
!!! ATACCTTATTATTGATGATG+GGG + Chr3:78117213-78117232 None:intergenic 25.0%
AAATCATAAGCAGAGTTATC+TGG + Chr3:78116923-78116942 None:intergenic 30.0%
AATCATAAGCAGAGTTATCT+GGG + Chr3:78116922-78116941 None:intergenic 30.0%
TAACCCCATCATCAATAATA+AGG - Chr3:78117207-78117226 MsG0380016027.01.T01:CDS 30.0%
TTTGTAGTGTTCAGTAACAA+TGG + Chr3:78116848-78116867 None:intergenic 30.0%
! GAAACCAAATTTGAGATTGA+AGG + Chr3:78117113-78117132 None:intergenic 30.0%
AAAACAGTTCAAGAGATGGA+AGG - Chr3:78117496-78117515 MsG0380016027.01.T01:CDS 35.0%
AAGAAACTCTCCTATGACAT+TGG - Chr3:78117022-78117041 MsG0380016027.01.T01:intron 35.0%
AGTAATGAGATCCAAATCCA+TGG + Chr3:78116999-78117018 None:intergenic 35.0%
ATGTCATAGGAGAGTTTCTT+TGG + Chr3:78117022-78117041 None:intergenic 35.0%
CTACCAATGTCTTTCAAAGA+TGG - Chr3:78117548-78117567 MsG0380016027.01.T01:CDS 35.0%
CTACTATGTGATACAGAGAT+GGG - Chr3:78117451-78117470 MsG0380016027.01.T01:CDS 35.0%
CTTCAAAACAGTTCAAGAGA+TGG - Chr3:78117492-78117511 MsG0380016027.01.T01:CDS 35.0%
GTAATGAGATCCAAATCCAT+GGG + Chr3:78116998-78117017 None:intergenic 35.0%
TACTATGTGATACAGAGATG+GGG - Chr3:78117452-78117471 MsG0380016027.01.T01:CDS 35.0%
! ACTACTCTCAAAGCACTATT+TGG - Chr3:78116945-78116964 MsG0380016027.01.T01:intron 35.0%
! TCTCCATCTTTGAAAGACAT+TGG + Chr3:78117554-78117573 None:intergenic 35.0%
!! ATTTGATCCTTGAAGGTGTT+GGG + Chr3:78117180-78117199 None:intergenic 35.0%
AATAGGGTCTTGGAACCAAA+GGG + Chr3:78116973-78116992 None:intergenic 40.0%
ATCCAAATCCATGGGAGAAT+AGG + Chr3:78116990-78117009 None:intergenic 40.0%
CATGCACATTTGATCCTTGA+AGG + Chr3:78117187-78117206 None:intergenic 40.0%
GCTACTATGTGATACAGAGA+TGG - Chr3:78117450-78117469 MsG0380016027.01.T01:CDS 40.0%
TCCAAATCCATGGGAGAATA+GGG + Chr3:78116989-78117008 None:intergenic 40.0%
TGTTCTTCTTCTGCTTCTTG+TGG + Chr3:78116818-78116837 None:intergenic 40.0%
! AGAAGCTCTAGTGACATGAA+TGG + Chr3:78116890-78116909 None:intergenic 40.0%
! CATTTGATCCTTGAAGGTGT+TGG + Chr3:78117181-78117200 None:intergenic 40.0%
!! ACAAGGTTGGTTCTAAGCTT+AGG - Chr3:78117068-78117087 MsG0380016027.01.T01:intron 40.0%
!! TTTGATCCTTGAAGGTGTTG+GGG + Chr3:78117179-78117198 None:intergenic 40.0%
AAGCACTATTTGGTCCCCTT+TGG - Chr3:78116955-78116974 MsG0380016027.01.T01:intron 45.0%
ACCCTATTCTCCCATGGATT+TGG - Chr3:78116985-78117004 MsG0380016027.01.T01:intron 45.0%
AGATGCAGTGTCAACAAGGT+TGG - Chr3:78117055-78117074 MsG0380016027.01.T01:intron 45.0%
AGCTAGATGCAGTGTCAACA+AGG - Chr3:78117051-78117070 MsG0380016027.01.T01:intron 45.0%
AGCTCCTAAGCCAATGTCAT+AGG + Chr3:78117035-78117054 None:intergenic 45.0%
ATAGGGTCTTGGAACCAAAG+GGG + Chr3:78116972-78116991 None:intergenic 45.0%
ATTTCTCCCCAACACCTTCA+AGG - Chr3:78117170-78117189 MsG0380016027.01.T01:CDS 45.0%
GAATAGGGTCTTGGAACCAA+AGG + Chr3:78116974-78116993 None:intergenic 45.0%
GGTAGCATCACATGAGCAAT+GGG + Chr3:78117533-78117552 None:intergenic 45.0%
GTAGCATCACATGAGCAATG+GGG + Chr3:78117532-78117551 None:intergenic 45.0%
TGGTAGCATCACATGAGCAA+TGG + Chr3:78117534-78117553 None:intergenic 45.0%
!! CTCTCCTATGACATTGGCTT+AGG - Chr3:78117028-78117047 MsG0380016027.01.T01:intron 45.0%
ATGTGATACAGAGATGGGGC+AGG - Chr3:78117456-78117475 MsG0380016027.01.T01:CDS 50.0%
TCCAAGACCCTATTCTCCCA+TGG - Chr3:78116979-78116998 MsG0380016027.01.T01:intron 50.0%
TCCATGGGAGAATAGGGTCT+TGG + Chr3:78116983-78117002 None:intergenic 50.0%
TGTGATACAGAGATGGGGCA+GGG - Chr3:78117457-78117476 MsG0380016027.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 78116797 78117580 78116797 ID=MsG0380016027.01;Name=MsG0380016027.01
Chr3 mRNA 78116797 78117580 78116797 ID=MsG0380016027.01.T01;Parent=MsG0380016027.01;Name=MsG0380016027.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|181
Chr3 exon 78117149 78117580 78117149 ID=MsG0380016027.01.T01:exon:18286;Parent=MsG0380016027.01.T01
Chr3 exon 78116797 78116910 78116797 ID=MsG0380016027.01.T01:exon:18285;Parent=MsG0380016027.01.T01
Chr3 CDS 78117149 78117580 78117149 ID=MsG0380016027.01.T01:cds;Parent=MsG0380016027.01.T01
Chr3 CDS 78116797 78116910 78116797 ID=MsG0380016027.01.T01:cds;Parent=MsG0380016027.01.T01
Gene Sequence

>MsG0380016027.01.T01

ATGAAATCTTTAGAAGAACCACAAGAAGCAGAAGAAGAACAACAACAACCATTGTTACTGAACACTACAAAAGTTGTTGAATATTTAGAACCATTCATGTCACTAGAGCTTCTCTGCAAATTCCCAGATAACTCTGCTTATGATTTTGACTACTCTCAAAGCACTATTTGGTCCCCTTTGGTTCCAAGACCCTATTCTCCCATGGATTTGGATCTCATTACTCCAAAGAAACTCTCCTATGACATTGGCTTAGGAGCTAGATGCAGTGTCAACAAGGTTGGTTCTAAGCTTAGGAAGAAGTTCACTTCTAATTCCTTCAATCTCAAATTTGGTTTCATTAAGAAACATAATAAGAACAACAAGATTGCTTCTGATTTCTCCCCAACACCTTCAAGGATCAAATGTGCATGTAACCCCATCATCAATAATAAGAGATGGGGCAGGGCATTGAAAGCTGCTTCAAAACAGTTCAAGAGATGGAAGGTGAAGAGAGACCCCATTGCTCATGTGATGCTACCAATGTCTTTCAAAGATGGAGATTTTTAA

Protein sequence

>MsG0380016027.01.T01

MKSLEEPQEAEEEQQQPLLLNTTKVVEYLEPFMSLELLCKFPDNSAYDFDYSQSTIWSPLVPRPYSPMDLDLITPKKLSYDIGLGARCSVNKVGSKLRKKFTSNSFNLKFGFIKKHNKNNKIASDFSPTPSRIKCACNPIINNKRWGRALKAASKQFKRWKVKRDPIAHVMLPMSFKDGDF*