AlfalfaGEDB | MsG0280007241.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007241.01

KEGG: K12737
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007241.01.T01	XP_024632468.2	97.826	92	2	0	1	92	408	499	1.05E-54	187

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007241.01.T01	Q6Q152	58.416	101	33	2	1	92	404	504	1.06E-29	112

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007241.01.T01	A0A396J5D4	97.826	92	2	0	1	92	408	499	5.04e-55	187

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047944.01	MsG0280007241.01	0.821938	3.094398e-53	2.553211e-50
MsG0080048964.01	MsG0280007241.01	0.805891	1.072248e-49	5.739254e-47
MsG0180000470.01	MsG0280007241.01	0.805892	1.071668e-49	5.736299e-47
MsG0180002488.01	MsG0280007241.01	0.805943	1.045649e-49	5.604500e-47
MsG0180003673.01	MsG0280007241.01	0.808182	3.511483e-50	1.994396e-47
MsG0180003686.01	MsG0280007241.01	0.809437	1.893469e-50	1.111664e-47
MsG0180003952.01	MsG0280007241.01	0.819629	1.050305e-52	8.125301e-50
MsG0180004295.01	MsG0280007241.01	0.808747	2.659914e-50	1.533168e-47
MsG0180004422.01	MsG0280007241.01	0.810075	1.380234e-50	8.241050e-48
MsG0180004465.01	MsG0280007241.01	0.800423	1.451604e-48	6.756682e-46
MsG0180005842.01	MsG0280007241.01	0.831424	1.682355e-55	1.824811e-52
MsG0180006134.01	MsG0280007241.01	0.814966	1.177173e-51	8.011833e-49
MsG0280006299.01	MsG0280007241.01	0.807932	3.970508e-50	2.240039e-47
MsG0280006375.01	MsG0280007241.01	0.811128	8.173513e-51	5.018238e-48
MsG0280006572.01	MsG0280007241.01	0.812375	4.377008e-51	2.778200e-48
MsG0280007088.01	MsG0280007241.01	0.801993	6.926306e-49	3.354513e-46
MsG0280007241.01	MsG0280007815.01	0.824582	7.467271e-54	6.642116e-51
MsG0280007241.01	MsG0280009024.01	0.801421	9.074634e-49	4.331843e-46
MsG0280007241.01	MsG0280009578.01	0.818827	1.599765e-52	1.210413e-49
MsG0280007241.01	MsG0280010722.01	0.809600	1.746229e-50	1.029756e-47
MsG0280007241.01	MsG0280011032.01	0.805874	1.081305e-49	5.785262e-47
MsG0280007241.01	MsG0280011175.01	0.804243	2.372417e-49	1.217020e-46
MsG0280007241.01	MsG0280011446.01	0.816670	4.907160e-52	3.499215e-49
MsG0280007241.01	MsG0280011472.01	0.802729	4.886663e-49	2.411635e-46
MsG0280007241.01	MsG0380011609.01	0.806303	8.781461e-50	4.749979e-47
MsG0280007241.01	MsG0380014177.01	0.809667	1.689500e-50	9.980575e-48
MsG0280007241.01	MsG0380014537.01	0.806007	1.013625e-49	5.441834e-47
MsG0280007241.01	MsG0380016581.01	0.811943	5.437214e-51	3.410948e-48
MsG0280007241.01	MsG0380016624.01	0.802041	6.770951e-49	3.283349e-46
MsG0280007241.01	MsG0380017543.01	0.820298	7.384671e-53	5.820411e-50
MsG0280007241.01	MsG0480021164.01	0.802733	4.877896e-49	2.407510e-46
MsG0280007241.01	MsG0480021274.01	0.803119	4.058974e-49	2.023000e-46
MsG0280007241.01	MsG0480021276.01	0.800348	1.503254e-48	6.983718e-46
MsG0280007241.01	MsG0480021536.01	0.807366	5.235948e-50	2.910821e-47
MsG0280007241.01	MsG0480021959.01	0.804264	2.348188e-49	1.205295e-46
MsG0280007241.01	MsG0480022259.01	0.807375	5.212834e-50	2.898654e-47
MsG0280007241.01	MsG0480022262.01	0.807016	6.210155e-50	3.421179e-47
MsG0280007241.01	MsG0480022551.01	0.803290	3.740147e-49	1.872337e-46
MsG0280007241.01	MsG0480023835.01	0.811986	5.321026e-51	3.341950e-48
MsG0280007241.01	MsG0580025022.01	0.800549	1.368362e-48	6.389276e-46
MsG0280007241.01	MsG0580025448.01	0.813326	2.709057e-51	1.763747e-48
MsG0280007241.01	MsG0580025638.01	0.803923	2.764360e-49	1.406690e-46
MsG0280007241.01	MsG0580025758.01	0.816083	6.639807e-52	4.659563e-49
MsG0280007241.01	MsG0580029826.01	0.836481	9.125166e-57	1.151428e-53
MsG0280007241.01	MsG0680031946.01	0.813697	2.245412e-51	1.476683e-48
MsG0280007241.01	MsG0680035573.01	0.801277	9.714495e-49	4.620590e-46
MsG0280007241.01	MsG0780037512.01	0.818963	1.489684e-52	1.131474e-49
MsG0280007241.01	MsG0780037919.01	0.803222	3.864410e-49	1.931188e-46
MsG0280007241.01	MsG0780038654.01	0.803132	4.032888e-49	2.010709e-46
MsG0280007241.01	MsG0780039144.01	0.817211	3.708786e-52	2.684210e-49
MsG0280007241.01	MsG0780041456.01	0.821157	4.687131e-53	3.783643e-50
MsG0280007241.01	MsG0880041836.01	0.818413	1.985527e-52	1.485372e-49
MsG0280007241.01	MsG0880043454.01	0.812419	4.280226e-51	2.720201e-48
MsG0280007241.01	MsG0880043509.01	0.820326	7.275806e-53	5.738878e-50
MsG0280007241.01	MsG0880045736.01	0.829885	4.006918e-55	4.155088e-52
MsG0280007241.01	MsG0880045937.01	0.816918	4.316892e-52	3.099283e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007241.01.T01	MTR_2g023120	90.476	42	4	0	1	42	408	449	4.05e-18	77.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007241.01.T01	AT4G33060	58.416	101	33	2	1	92	404	504	1.08e-30	112

Find 23 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTGTACATGACCCACTTT+TGG	0.294594	2:+12442235	MsG0280007241.01.T01:CDS
TGTCTCCTTTGCTGATACCT+TGG	0.404713	2:-12441794	None:intergenic
TCATGTACAACATAATCATT+TGG	0.427856	2:-12442224	None:intergenic
AAACTGACCTTGCCAACTCC+GGG	0.447636	2:-12441892	None:intergenic
AGACGAGAACGGGAATGGGC+TGG	0.448205	2:+12442302	MsG0280007241.01.T01:CDS
GACGAGAACGGGAATGGGCT+GGG	0.454947	2:+12442303	MsG0280007241.01.T01:CDS
CAAACAAAAGAGACGAGAAC+GGG	0.471510	2:+12442292	MsG0280007241.01.T01:CDS
ATGTTTAGTTTCAACAGTTG+TGG	0.474502	2:-12441818	None:intergenic
TGTTTAGTTTCAACAGTTGT+GGG	0.493078	2:-12441817	None:intergenic
AAAACTGACCTTGCCAACTC+CGG	0.499053	2:-12441893	None:intergenic
GACACCAGCAGAATCAAGTA+AGG	0.500281	2:-12442331	None:intergenic
AAAAGAGACGAGAACGGGAA+TGG	0.518777	2:+12442297	MsG0280007241.01.T01:CDS
AAAGAGACGAGAACGGGAAT+GGG	0.523623	2:+12442298	MsG0280007241.01.T01:CDS
CCAAACAAAAGAGACGAGAA+CGG	0.567719	2:+12442291	MsG0280007241.01.T01:CDS
TAAAACCAAGGTATCAGCAA+AGG	0.594376	2:+12441789	MsG0280007241.01.T01:CDS
GATCCTCTTTGCGAGACATG+TGG	0.601203	2:-12442202	None:intergenic
ACTGACCTTGCCAACTCCGG+GGG	0.609005	2:-12441890	None:intergenic
GTGACGAGGAATTAACTGAC+TGG	0.616573	2:+12441845	MsG0280007241.01.T01:CDS
ACTTGAGAAGTTTAAAACCA+AGG	0.622239	2:+12441777	MsG0280007241.01.T01:CDS
AACTGACCTTGCCAACTCCG+GGG	0.637540	2:-12441891	None:intergenic
GGACCACATGTCTCGCAAAG+AGG	0.660428	2:+12442199	MsG0280007241.01.T01:intron
AACTAAACATGCTAGTGACG+AGG	0.668903	2:+12441831	MsG0280007241.01.T01:CDS
ACACCAGCAGAATCAAGTAA+GGG	0.677787	2:-12442330	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAAAAACTTCAATGTAAA+TGG	-	Chr2:12441955-12441974	None:intergenic	15.0%
!!	AAAAATTTCTGTTAATATGC+AGG	+	Chr2:12442178-12442197	MsG0280007241.01.T01:intron	20.0%
!!	ATTGTAGAAAAGAATTAACT+TGG	-	Chr2:12442081-12442100	None:intergenic	20.0%
!!!	ATTAGTTTTATAACCAAATG+TGG	+	Chr2:12442006-12442025	MsG0280007241.01.T01:intron	20.0%
!	AGTGAAATGATTTCATATGA+TGG	-	Chr2:12441930-12441949	None:intergenic	25.0%
!	TCATGTACAACATAATCATT+TGG	-	Chr2:12442227-12442246	None:intergenic	25.0%
!!	ATTTCATATGATGGAATGAA+AGG	-	Chr2:12441921-12441940	None:intergenic	25.0%
!!!	ATTTTTTAAGACAGCAGAAA+AGG	-	Chr2:12442164-12442183	None:intergenic	25.0%
!!!	TTTTTTAAGACAGCAGAAAA+GGG	-	Chr2:12442163-12442182	None:intergenic	25.0%
!	ACTTGAGAAGTTTAAAACCA+AGG	+	Chr2:12441777-12441796	MsG0280007241.01.T01:CDS	30.0%
!	ATGTTTAGTTTCAACAGTTG+TGG	-	Chr2:12441821-12441840	None:intergenic	30.0%
!	GTAGAAAAGAATTAACTTGG+TGG	-	Chr2:12442078-12442097	None:intergenic	30.0%
!	TGTTTAGTTTCAACAGTTGT+GGG	-	Chr2:12441820-12441839	None:intergenic	30.0%
!	CAACACAAGTCTCTTTTAGA+CGG	+	Chr2:12442106-12442125	MsG0280007241.01.T01:intron	35.0%
!	CTTGGTGGTTAGAATTCAAA+AGG	-	Chr2:12442063-12442082	None:intergenic	35.0%
!	TAAAACCAAGGTATCAGCAA+AGG	+	Chr2:12441789-12441808	MsG0280007241.01.T01:CDS	35.0%
	AACTAAACATGCTAGTGACG+AGG	+	Chr2:12441831-12441850	MsG0280007241.01.T01:CDS	40.0%
	AACTAACGTGTCTCCACATT+TGG	-	Chr2:12442022-12442041	None:intergenic	40.0%
	CAAACAAAAGAGACGAGAAC+GGG	+	Chr2:12442292-12442311	MsG0280007241.01.T01:CDS	40.0%
	CCAAACAAAAGAGACGAGAA+CGG	+	Chr2:12442291-12442310	MsG0280007241.01.T01:CDS	40.0%
	TTTAGACGGAGTTGTTCGTA+TGG	+	Chr2:12442120-12442139	MsG0280007241.01.T01:intron	40.0%
!	CTTTCCCCTTTTCCAAAAGT+GGG	-	Chr2:12442250-12442269	None:intergenic	40.0%
!	GTTACCTTGAAATTTTCCCC+CGG	+	Chr2:12441874-12441893	MsG0280007241.01.T01:CDS	40.0%
!	TCTTTCCCCTTTTCCAAAAG+TGG	-	Chr2:12442251-12442270	None:intergenic	40.0%
!	TGTTGTACATGACCCACTTT+TGG	+	Chr2:12442235-12442254	MsG0280007241.01.T01:CDS	40.0%
!!	ACATGACCCACTTTTGGAAA+AGG	+	Chr2:12442241-12442260	MsG0280007241.01.T01:CDS	40.0%
!!	ATGACCCACTTTTGGAAAAG+GGG	+	Chr2:12442243-12442262	MsG0280007241.01.T01:CDS	40.0%
!!	CATGACCCACTTTTGGAAAA+GGG	+	Chr2:12442242-12442261	MsG0280007241.01.T01:CDS	40.0%
	AAAACTGACCTTGCCAACTC+CGG	-	Chr2:12441896-12441915	None:intergenic	45.0%
	AAAAGAGACGAGAACGGGAA+TGG	+	Chr2:12442297-12442316	MsG0280007241.01.T01:CDS	45.0%
	AAAGAGACGAGAACGGGAAT+GGG	+	Chr2:12442298-12442317	MsG0280007241.01.T01:CDS	45.0%
	AACTCCGGGGGAAAATTTCA+AGG	-	Chr2:12441881-12441900	None:intergenic	45.0%
	GTGACGAGGAATTAACTGAC+TGG	+	Chr2:12441845-12441864	MsG0280007241.01.T01:CDS	45.0%
	TGTCTCCTTTGCTGATACCT+TGG	-	Chr2:12441797-12441816	None:intergenic	45.0%
!	CCGTTCTCGTCTCTTTTGTT+TGG	-	Chr2:12442294-12442313	None:intergenic	45.0%
!	TTGAAATTTTCCCCCGGAGT+TGG	+	Chr2:12441880-12441899	MsG0280007241.01.T01:CDS	45.0%
	AAACTGACCTTGCCAACTCC+GGG	-	Chr2:12441895-12441914	None:intergenic	50.0%
	GATCCTCTTTGCGAGACATG+TGG	-	Chr2:12442205-12442224	None:intergenic	50.0%
	AACTGACCTTGCCAACTCCG+GGG	-	Chr2:12441894-12441913	None:intergenic	55.0%
	GGACCACATGTCTCGCAAAG+AGG	+	Chr2:12442199-12442218	MsG0280007241.01.T01:intron	55.0%
!	ATTTTCCCCCGGAGTTGGCA+AGG	+	Chr2:12441885-12441904	MsG0280007241.01.T01:CDS	55.0%
	ACTGACCTTGCCAACTCCGG+GGG	-	Chr2:12441893-12441912	None:intergenic	60.0%
	AGACGAGAACGGGAATGGGC+TGG	+	Chr2:12442302-12442321	MsG0280007241.01.T01:CDS	60.0%
!	GACGAGAACGGGAATGGGCT+GGG	+	Chr2:12442303-12442322	MsG0280007241.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	12441769	12442340	12441769	ID=MsG0280007241.01;Name=MsG0280007241.01
Chr2	mRNA	12441769	12442340	12441769	ID=MsG0280007241.01.T01;Parent=MsG0280007241.01;Name=MsG0280007241.01.T01;_AED=0.50;_eAED=1.00;_QI=0\|0\|0\|1\|0\|0\|2\|0\|92
Chr2	exon	12441769	12441906	12441769	ID=MsG0280007241.01.T01:exon:2934;Parent=MsG0280007241.01.T01
Chr2	exon	12442200	12442340	12442200	ID=MsG0280007241.01.T01:exon:2935;Parent=MsG0280007241.01.T01
Chr2	CDS	12441769	12441906	12441769	ID=MsG0280007241.01.T01:cds;Parent=MsG0280007241.01.T01
Chr2	CDS	12442200	12442340	12442200	ID=MsG0280007241.01.T01:cds;Parent=MsG0280007241.01.T01

Gene Sequence	>MsG0280007241.01.T01 TTGGCAAAACTTGAGAAGTTTAAAACCAAGGTATCAGCAAAGGAGACACCCACAACTGTTGAAACTAAACATGCTAGTGACGAGGAATTAACTGACTGGAGTAGTGTTACCTTGAAATTTTCCCCCGGAGTTGGCAAGGACCACATGTCTCGCAAAGAGGATCCAAATGATTATGTTGTACATGACCCACTTTTGGAAAAGGGGAAAGAGAAGTTTAATAAGATGCAAGCCAAACAAAAGAGACGAGAACGGGAATGGGCTGGGAGATCCCTTACTTGA
Protein sequence	>MsG0280007241.01.T01 LAKLEKFKTKVSAKETPTTVETKHASDEELTDWSSVTLKFSPGVGKDHMSRKEDPNDYVVHDPLLEKGKEKFNKMQAKQKRREREWAGRSLT*