AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003124.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003124.01.T01 MTR_1g054750 93.960 149 6 1 1 149 22 167 1.98e-99 285
MsG0180003124.01.T01 MTR_1g054765 73.548 155 32 5 1 149 3 154 1.69e-72 216
MsG0180003124.01.T01 MTR_1g083950 37.241 145 75 5 2 146 10 138 6.55e-21 84.3
MsG0180003124.01.T01 MTR_1g088640 35.294 136 73 4 4 138 15 136 7.53e-18 76.6
MsG0180003124.01.T01 MTR_5g065980 31.757 148 91 4 3 146 10 151 5.75e-17 74.3
MsG0180003124.01.T01 MTR_1g087200 30.000 140 83 4 2 138 7 134 1.26e-16 73.2
MsG0180003124.01.T01 MTR_5g065980 31.429 140 89 3 3 138 10 146 3.90e-16 72.8
MsG0180003124.01.T01 MTR_5g065980 32.000 150 90 5 3 146 10 153 9.35e-16 71.2
MsG0180003124.01.T01 MTR_1g088640 34.532 139 73 5 4 138 15 139 1.05e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003124.01.T01 AT3G11930 50.336 149 69 3 2 149 34 178 6.92e-50 159
MsG0180003124.01.T01 AT3G11930 50.667 150 68 4 2 149 34 179 1.03e-48 156
MsG0180003124.01.T01 AT3G11930 50.331 151 68 4 2 149 34 180 1.28e-48 156
MsG0180003124.01.T01 AT3G11930 51.799 139 64 3 2 138 34 171 3.69e-47 153
MsG0180003124.01.T01 AT3G58450 46.939 147 74 2 3 149 32 174 1.41e-41 138
MsG0180003124.01.T01 AT3G58450 46.259 147 68 4 3 149 32 167 1.09e-37 128
MsG0180003124.01.T01 AT1G09740 35.616 146 84 3 3 146 10 147 1.21e-18 79.0
MsG0180003124.01.T01 AT1G09740 35.616 146 84 3 3 146 10 147 1.21e-18 79.0
MsG0180003124.01.T01 AT3G62550 30.935 139 82 4 2 138 8 134 2.17e-14 67.4
MsG0180003124.01.T01 AT2G47710 27.941 136 85 3 4 138 11 134 3.35e-13 64.3
MsG0180003124.01.T01 AT1G68300 29.452 146 86 4 2 146 11 140 2.77e-12 61.6
MsG0180003124.01.T01 AT3G17020 31.724 145 77 4 2 138 8 138 3.19e-11 58.9

Find 50 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATTATGTGCACCCTAT+TGG 0.235333 1:-56985898 MsG0180003124.01.T01:CDS
GTGCACATAATTGTGGAAAA+TGG 0.245181 1:+56985906 None:intergenic
TTGAATGAGATTGATGGTTT+TGG 0.278641 1:-56982275 MsG0180003124.01.T01:CDS
AGCTCTATTTATAGGTTCTA+AGG 0.312656 1:-56982342 MsG0180003124.01.T01:CDS
CCAGCACCACCTGGTCCAAT+AGG 0.340944 1:+56985883 None:intergenic
AGGAAGAGAGCTCTATTTAT+AGG 0.341464 1:-56982350 MsG0180003124.01.T01:CDS
AATCATGGGTAGCCGTGGCC+TGG 0.369904 1:-56984275 MsG0180003124.01.T01:CDS
ATTGGTTTAATGCACTTTGA+TGG 0.371533 1:-56982302 MsG0180003124.01.T01:CDS
CCTATTGGACCAGGTGGTGC+TGG 0.410297 1:-56985883 MsG0180003124.01.T01:intron
GCAAGTTGATCTCTTAATCA+TGG 0.412365 1:-56984290 MsG0180003124.01.T01:CDS
TTATCCAGCATCTGTGGTTG+TGG 0.413861 1:-56985584 MsG0180003124.01.T01:CDS
GCCTGACAAATCATTTCCCT+TGG 0.420407 1:+56984325 None:intergenic
TTGGTTTAATGCACTTTGAT+GGG 0.434028 1:-56982301 MsG0180003124.01.T01:CDS
ATCATGGGTAGCCGTGGCCT+GGG 0.449797 1:-56984274 MsG0180003124.01.T01:CDS
CCCAAGGGAAATGATTTGTC+AGG 0.453085 1:-56984326 MsG0180003124.01.T01:CDS
TGCACATAATTGTGGAAAAT+GGG 0.455217 1:+56985907 None:intergenic
TAACTGCTGATCTTTCTTGC+TGG 0.457940 1:+56985545 None:intergenic
GGGGATTTGAATGAGATTGA+TGG 0.463422 1:-56982281 MsG0180003124.01.T01:CDS
GCAGAAAGTGTAATTCTCAA+TGG 0.481801 1:-56984352 MsG0180003124.01.T01:CDS
GAATCCACAACCACAGATGC+TGG 0.485403 1:+56985580 None:intergenic
AGGAACATCATCTGAAAATG+AGG 0.493972 1:-56985956 MsG0180003124.01.T01:CDS
GAAGCTTCTATGCTCTTAAG+TGG 0.498753 1:-56986017 MsG0180003124.01.T01:CDS
GTGGCCTGGGCACACTCAAA+AGG 0.501846 1:-56984261 MsG0180003124.01.T01:intron
GCAAATGTGCAAGGATAAGC+AGG 0.503833 1:-56985506 MsG0180003124.01.T01:intron
CCTGACAAATCATTTCCCTT+GGG 0.505400 1:+56984326 None:intergenic
TTCAGATGATGTTCCTTCCA+TGG 0.510145 1:+56985963 None:intergenic
ATCAGCAGTTATTCTTTCGC+GGG 0.511531 1:-56985533 MsG0180003124.01.T01:CDS
CAGCACCACCTGGTCCAATA+GGG 0.517598 1:+56985884 None:intergenic
TAAAGTTGAAGAGATTATCA+AGG 0.517712 1:+56985992 None:intergenic
ATCATCTGAAAATGAGGGAA+TGG 0.523826 1:-56985950 MsG0180003124.01.T01:CDS
GATCAGCAGTTATTCTTTCG+CGG 0.533226 1:-56985534 MsG0180003124.01.T01:CDS
CTTCAACTTTATGACTGCCA+TGG 0.538211 1:-56985980 MsG0180003124.01.T01:CDS
CTCTTAATCATGGGTAGCCG+TGG 0.547180 1:-56984280 MsG0180003124.01.T01:CDS
CAATAGGGTGCACATAATTG+TGG 0.554577 1:+56985899 None:intergenic
GCGGGCTTTGCAAATGTGCA+AGG 0.556519 1:-56985515 MsG0180003124.01.T01:CDS
CAAGTTGATCTCTTAATCAT+GGG 0.558572 1:-56984289 MsG0180003124.01.T01:CDS
AACTTTATGACTGCCATGGA+AGG 0.559729 1:-56985976 MsG0180003124.01.T01:CDS
GGAACATCATCTGAAAATGA+GGG 0.582944 1:-56985955 MsG0180003124.01.T01:CDS
TGGTTTAATGCACTTTGATG+GGG 0.587373 1:-56982300 MsG0180003124.01.T01:CDS
AAAGTTGAAGAGATTATCAA+GGG 0.605580 1:+56985993 None:intergenic
TAATTCTCAATGGAGACCCA+AGG 0.620896 1:-56984342 MsG0180003124.01.T01:CDS
GTTGTGGATTCAGTGAAGAA+AGG 0.627405 1:-56985568 MsG0180003124.01.T01:CDS
AGATACGAACCAGCACCACC+TGG 0.633023 1:+56985874 None:intergenic
TATGTGCACCCTATTGGACC+AGG 0.634058 1:-56985892 MsG0180003124.01.T01:CDS
AAGCTTCTATGCTCTTAAGT+GGG 0.645712 1:-56986016 MsG0180003124.01.T01:CDS
GTGGCAATAGATGAGAGTGA+TGG 0.658459 1:-56986039 MsG0180003124.01.T01:CDS
AATTCTCAATGGAGACCCAA+GGG 0.664861 1:-56984341 MsG0180003124.01.T01:CDS
GCTATTGATGAAGGTGATGG+TGG 0.671418 1:-56986058 None:intergenic
AGAGCTATTGATGAAGGTGA+TGG 0.678882 1:-56986061 None:intergenic
GTGCACCCTATTGGACCAGG+TGG 0.681996 1:-56985889 MsG0180003124.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAGCAAAAAAATT+TGG - Chr1:56983733-56983752 MsG0180003124.01.T01:intron 10.0%
!! AATACAAAAAAAATTAGATT+AGG + Chr1:56985587-56985606 None:intergenic 10.0%
!! AATTGATAAAAATTAAAAGA+TGG + Chr1:56983822-56983841 None:intergenic 10.0%
!! ACAATTGAAATAAAATTAAA+TGG - Chr1:56982950-56982969 MsG0180003124.01.T01:intron 10.0%
!! ATAAAAAAGCAAAAAAATTT+GGG - Chr1:56983734-56983753 MsG0180003124.01.T01:intron 10.0%
!! ATACAAAAAAAATTAGATTA+GGG + Chr1:56985586-56985605 None:intergenic 10.0%
!! ATATTTAATTGTTAAGTTAT+AGG + Chr1:56983402-56983421 None:intergenic 10.0%
!! GATAATTTGATATATTTATT+GGG - Chr1:56984551-56984570 MsG0180003124.01.T01:intron 10.0%
!! TAAACAAAATATAGAAAATA+TGG + Chr1:56984113-56984132 None:intergenic 10.0%
!! TATTTAATTGTTAAGTTATA+GGG + Chr1:56983401-56983420 None:intergenic 10.0%
!! TTTAGTTAAATTAAAAAGAA+AGG + Chr1:56984593-56984612 None:intergenic 10.0%
!!! AAAAAGTTGATATATTTATA+AGG + Chr1:56984156-56984175 None:intergenic 10.0%
!!! AATTTTGAAATGATTAAAAA+AGG - Chr1:56985149-56985168 MsG0180003124.01.T01:intron 10.0%
!!! ATATTTTCTATATTTTGTTT+AGG - Chr1:56984112-56984131 MsG0180003124.01.T01:intron 10.0%
!!! ATTAAAATAGAAAATTTTTG+AGG + Chr1:56984216-56984235 None:intergenic 10.0%
!!! GTTAAAAAATTCTAATTTTT+TGG - Chr1:56984415-56984434 MsG0180003124.01.T01:intron 10.0%
!!! TAAAATTTTTAGATTTTTTG+AGG - Chr1:56984364-56984383 MsG0180003124.01.T01:intron 10.0%
!!! TATTTTCTATATTTTGTTTA+GGG - Chr1:56984113-56984132 MsG0180003124.01.T01:intron 10.0%
!!! TTTAATTTTTTTAAAGAAAG+GGG - Chr1:56984962-56984981 MsG0180003124.01.T01:intron 10.0%
!!! TTTTTGATATATATATAAAG+CGG - Chr1:56985607-56985626 MsG0180003124.01.T01:intron 10.0%
!! AAAAGTTAAATAAAATCAAG+AGG + Chr1:56985094-56985113 None:intergenic 15.0%
!! AGTTGAAGAAATTTATTATA+AGG + Chr1:56984187-56984206 None:intergenic 15.0%
!! GAATTCTTAAAAAATAATCT+GGG + Chr1:56983046-56983065 None:intergenic 15.0%
!! GGATAATTTGATATATTTAT+TGG - Chr1:56984550-56984569 MsG0180003124.01.T01:intron 15.0%
!! TCAATAAAATGTAGATAATT+AGG + Chr1:56985179-56985198 None:intergenic 15.0%
!! TGTACATAGTAATAATTTAA+GGG + Chr1:56984762-56984781 None:intergenic 15.0%
!! TTTCTCATTTATATTTACAT+TGG - Chr1:56985667-56985686 MsG0180003124.01.T01:intron 15.0%
!!! AAATGACACTTATTTTAAAA+CGG + Chr1:56984662-56984681 None:intergenic 15.0%
!!! ATGAGAAATACTATTTTTTA+GGG - Chr1:56983646-56983665 MsG0180003124.01.T01:intron 15.0%
!!! GTTAAAATTGATATTTTCAA+TGG + Chr1:56983442-56983461 None:intergenic 15.0%
!!! TATGAGAAATACTATTTTTT+AGG - Chr1:56983645-56983664 MsG0180003124.01.T01:intron 15.0%
!!! TTATCTACATTTTATTGATT+TGG - Chr1:56985181-56985200 MsG0180003124.01.T01:intron 15.0%
!!! TTTTTCATTATGATACAATT+TGG + Chr1:56984791-56984810 None:intergenic 15.0%
!! AAATATCAAAATTGCTTAGT+CGG + Chr1:56982577-56982596 None:intergenic 20.0%
!! AATGCAAGAAAATAAAAGAA+AGG - Chr1:56985771-56985790 MsG0180003124.01.T01:intron 20.0%
!! ATGCAAGAAAATAAAAGAAA+GGG - Chr1:56985772-56985791 MsG0180003124.01.T01:intron 20.0%
!! CCTATTTATAAGAAAAAGTT+TGG + Chr1:56983342-56983361 None:intergenic 20.0%
!! CTCAAATTTCTCTTATAAAT+TGG + Chr1:56983260-56983279 None:intergenic 20.0%
!! GGAATTCTTAAAAAATAATC+TGG + Chr1:56983047-56983066 None:intergenic 20.0%
!! GTGTACATAGTAATAATTTA+AGG + Chr1:56984763-56984782 None:intergenic 20.0%
!! TACATATGAGTTTACTTAAA+AGG + Chr1:56984249-56984268 None:intergenic 20.0%
!! TCTAAAGAAAGATATTCAAA+GGG + Chr1:56982488-56982507 None:intergenic 20.0%
!! TTTCAATTTGGAAAACTTAA+TGG + Chr1:56983491-56983510 None:intergenic 20.0%
!!! AAAATAGCTTTGTTACATAT+TGG + Chr1:56984818-56984837 None:intergenic 20.0%
!!! AAAATTTTAAAGGGACTTAA+AGG - Chr1:56984529-56984548 MsG0180003124.01.T01:intron 20.0%
!!! CCAAACTTTTTCTTATAAAT+AGG - Chr1:56983339-56983358 MsG0180003124.01.T01:intron 20.0%
!!! GTTTTGGAAAATTAAACTAA+CGG - Chr1:56985505-56985524 MsG0180003124.01.T01:intron 20.0%
!!! TGAGAAATACTATTTTTTAG+GGG - Chr1:56983647-56983666 MsG0180003124.01.T01:intron 20.0%
!!! TGTCGAAATCAATTTTATTT+GGG - Chr1:56983850-56983869 MsG0180003124.01.T01:intron 20.0%
!!! TTCTAAAGAAAGATATTCAA+AGG + Chr1:56982489-56982508 None:intergenic 20.0%
!!! TTGCTTGCTTTTTTTAAAAA+TGG - Chr1:56985126-56985145 MsG0180003124.01.T01:intron 20.0%
!!! TTTGAATGTTTTTGTTGTTT+TGG - Chr1:56985489-56985508 MsG0180003124.01.T01:intron 20.0%
! AAAGTTGAAGAGATTATCAA+GGG + Chr1:56982329-56982348 None:intergenic 25.0%
! AGAAGACTAATAAAAGAACA+AGG + Chr1:56983570-56983589 None:intergenic 25.0%
! ATTTCTCTTATAAATTGGAC+CGG + Chr1:56983255-56983274 None:intergenic 25.0%
! ATTTGGAAAACTTAATGGAA+TGG + Chr1:56983486-56983505 None:intergenic 25.0%
! CTAAAGAAAGATATTCAAAG+GGG + Chr1:56982487-56982506 None:intergenic 25.0%
! GGTGTAATTGAAGAAAATAA+GGG + Chr1:56983159-56983178 None:intergenic 25.0%
! GTTGAAGTGATAAAATATTG+AGG + Chr1:56982709-56982728 None:intergenic 25.0%
! TAAAGTTGAAGAGATTATCA+AGG + Chr1:56982330-56982349 None:intergenic 25.0%
! TCAACAAAAGTTAATGTATC+TGG - Chr1:56983280-56983299 MsG0180003124.01.T01:intron 25.0%
! TTTGGAAAACTTAATGGAAT+GGG + Chr1:56983485-56983504 None:intergenic 25.0%
!! AAATGAAGTACTAACAAACT+TGG - Chr1:56984628-56984647 MsG0180003124.01.T01:intron 25.0%
!! AAATTAGATTAGGGATTTTG+TGG + Chr1:56985577-56985596 None:intergenic 25.0%
!! AACCCTAAATCTTTTCAATA+TGG + Chr1:56985008-56985027 None:intergenic 25.0%
!! AATTGAGTTTGGATTTTCAT+AGG - Chr1:56982994-56983013 MsG0180003124.01.T01:intron 25.0%
!! ACATGAGTTGACTTAAAAAT+AGG - Chr1:56984488-56984507 MsG0180003124.01.T01:intron 25.0%
!! ACCCTAAATCTTTTCAATAT+GGG + Chr1:56985007-56985026 None:intergenic 25.0%
!! ATTGAGTTTGGATTTTCATA+GGG - Chr1:56982995-56983014 MsG0180003124.01.T01:intron 25.0%
!! CATGAGTTGACTTAAAAATA+GGG - Chr1:56984489-56984508 MsG0180003124.01.T01:intron 25.0%
!! CTTTTTCTTATAAATAGGAC+CGG - Chr1:56983344-56983363 MsG0180003124.01.T01:intron 25.0%
!! GTGTCGAAATCAATTTTATT+TGG - Chr1:56983849-56983868 MsG0180003124.01.T01:intron 25.0%
!! GTTGATGAAAAATGGAAAAT+TGG - Chr1:56985999-56986018 MsG0180003124.01.T01:CDS 25.0%
!! TAGAGTACTACGAATATTTA+GGG + Chr1:56985065-56985084 None:intergenic 25.0%
!! TATTGAAAAGATTTAGGGTT+CGG - Chr1:56985008-56985027 MsG0180003124.01.T01:intron 25.0%
!! TGGTGACTGAAAATTTTAAA+GGG - Chr1:56984520-56984539 MsG0180003124.01.T01:intron 25.0%
!! TTTAAAATGAAAAAGCCAAG+TGG - Chr1:56985313-56985332 MsG0180003124.01.T01:intron 25.0%
!!! AGGTATGATTTGTTTGTTTT+AGG + Chr1:56983550-56983569 None:intergenic 25.0%
!!! CTACATTTTATTGATTTGGA+AGG - Chr1:56985185-56985204 MsG0180003124.01.T01:intron 25.0%
!!! GGTATGATTTGTTTGTTTTA+GGG + Chr1:56983549-56983568 None:intergenic 25.0%
!!! GTCGAAATCAATTTTATTTG+GGG - Chr1:56983851-56983870 MsG0180003124.01.T01:intron 25.0%
!!! TAAGGGTACTATTAGAAAAA+TGG + Chr1:56984745-56984764 None:intergenic 25.0%
AAAGATATTCAAAGGGGATA+AGG + Chr1:56982481-56982500 None:intergenic 30.0%
AAGTGACAAAAATTACCGAA+GGG - Chr1:56985802-56985821 MsG0180003124.01.T01:intron 30.0%
AATGGGTAATTGATCTACAA+AGG - Chr1:56983206-56983225 MsG0180003124.01.T01:intron 30.0%
ACCCCATATTGAAAAGATTT+AGG - Chr1:56985002-56985021 MsG0180003124.01.T01:intron 30.0%
ACTTAAGAAATGAACACCTA+AGG - Chr1:56983613-56983632 MsG0180003124.01.T01:intron 30.0%
AGAAAATAAGGGAATAGCTT+TGG + Chr1:56983148-56983167 None:intergenic 30.0%
AGCTCTATTTATAGGTTCTA+AGG - Chr1:56985977-56985996 MsG0180003124.01.T01:CDS 30.0%
ATGATTTATCTAGCCAAATC+TGG - Chr1:56983911-56983930 MsG0180003124.01.T01:intron 30.0%
ATGGGTAATTGATCTACAAA+GGG - Chr1:56983207-56983226 MsG0180003124.01.T01:intron 30.0%
ATGTTCATTAATGGAATTGC+AGG - Chr1:56983939-56983958 MsG0180003124.01.T01:intron 30.0%
ATTAAATGGATCACTTCAAG+TGG - Chr1:56982964-56982983 MsG0180003124.01.T01:intron 30.0%
CCAGCAATTAGAATGTATTT+AGG + Chr1:56985694-56985713 None:intergenic 30.0%
CCCCATATTGAAAAGATTTA+GGG - Chr1:56985003-56985022 MsG0180003124.01.T01:intron 30.0%
CCTAAATACATTCTAATTGC+TGG - Chr1:56985691-56985710 MsG0180003124.01.T01:intron 30.0%
CTGGTGAAAATGTTCATTAA+TGG - Chr1:56983930-56983949 MsG0180003124.01.T01:intron 30.0%
CTTTGACCAAGAAAAACAAA+GGG + Chr1:56983180-56983199 None:intergenic 30.0%
GATCACTTGATTGAAAATCA+AGG - Chr1:56985435-56985454 MsG0180003124.01.T01:intron 30.0%
GGGTGTAATTGAAGAAAATA+AGG + Chr1:56983160-56983179 None:intergenic 30.0%
TAGATCAATTACCCATTATC+TGG + Chr1:56983203-56983222 None:intergenic 30.0%
TAGGTGTTCATTTCTTAAGT+GGG + Chr1:56983613-56983632 None:intergenic 30.0%
TGCACATAATTGTGGAAAAT+GGG + Chr1:56982415-56982434 None:intergenic 30.0%
TTAAAAATAGGGACCAAAAG+TGG - Chr1:56984500-56984519 MsG0180003124.01.T01:intron 30.0%
TTAGGTGTTCATTTCTTAAG+TGG + Chr1:56983614-56983633 None:intergenic 30.0%
TTGGTTTAATGCACTTTGAT+GGG - Chr1:56986018-56986037 MsG0180003124.01.T01:CDS 30.0%
! AAATTTTCAGTCACCACTTT+TGG + Chr1:56984516-56984535 None:intergenic 30.0%
! ATGAACATTTTCACCAGATT+TGG + Chr1:56983927-56983946 None:intergenic 30.0%
! ATTGAAAAGATTTAGGGTTC+GGG - Chr1:56985009-56985028 MsG0180003124.01.T01:intron 30.0%
! ATTGGTTTAATGCACTTTGA+TGG - Chr1:56986017-56986036 MsG0180003124.01.T01:CDS 30.0%
! CCCTAAATCTTTTCAATATG+GGG + Chr1:56985006-56985025 None:intergenic 30.0%
! CTGGTCAAATGTTTTCAATT+TGG + Chr1:56983503-56983522 None:intergenic 30.0%
! GTAGAGTACTACGAATATTT+AGG + Chr1:56985066-56985085 None:intergenic 30.0%
! GTGGTGACTGAAAATTTTAA+AGG - Chr1:56984519-56984538 MsG0180003124.01.T01:intron 30.0%
! TTAAGAAGAGAGTATATACG+TGG + Chr1:56983890-56983909 None:intergenic 30.0%
!! CAAGTTGATCTCTTAATCAT+GGG - Chr1:56984030-56984049 MsG0180003124.01.T01:intron 30.0%
!! GTTCTAAGGTTGATGAAAAA+TGG - Chr1:56985991-56986010 MsG0180003124.01.T01:CDS 30.0%
!! TCAACAAAAGTTGATGTATC+CGG + Chr1:56983319-56983338 None:intergenic 30.0%
!! TGATATTTTCAATGGCTTGT+TGG + Chr1:56983434-56983453 None:intergenic 30.0%
!! TTCGGTAATTTTTGTCACTT+CGG + Chr1:56985802-56985821 None:intergenic 30.0%
!! TTGAATGAGATTGATGGTTT+TGG - Chr1:56986044-56986063 MsG0180003124.01.T01:CDS 30.0%
!!! ATGATTTGTTTGTTTTAGGG+AGG + Chr1:56983546-56983565 None:intergenic 30.0%
!!! ATTACACCCTTTGTTTTTCT+TGG - Chr1:56983171-56983190 MsG0180003124.01.T01:intron 30.0%
!!! GACACTTATTTTAAAACGGA+TGG + Chr1:56984658-56984677 None:intergenic 30.0%
!!! GATATTTTCAATGGCTTGTT+GGG + Chr1:56983433-56983452 None:intergenic 30.0%
!!! GATTTTCATAGGGTTTGTTA+AGG - Chr1:56983005-56983024 MsG0180003124.01.T01:intron 30.0%
!!! TGATTTGTTTGTTTTAGGGA+GGG + Chr1:56983545-56983564 None:intergenic 30.0%
AAGCTTCTATGCTCTTAAGT+GGG - Chr1:56982303-56982322 MsG0180003124.01.T01:CDS 35.0%
ACCTAATGCAACTAGAAAAC+TGG + Chr1:56983522-56983541 None:intergenic 35.0%
AGGAACATCATCTGAAAATG+AGG - Chr1:56982363-56982382 MsG0180003124.01.T01:intron 35.0%
AGGAAGAGAGCTCTATTTAT+AGG - Chr1:56985969-56985988 MsG0180003124.01.T01:CDS 35.0%
ATCATCTGAAAATGAGGGAA+TGG - Chr1:56982369-56982388 MsG0180003124.01.T01:intron 35.0%
ATCTGGTTAAAAGTTCAGTC+CGG - Chr1:56983297-56983316 MsG0180003124.01.T01:intron 35.0%
GAAAACTTAATGGAATGGGT+GGG + Chr1:56983481-56983500 None:intergenic 35.0%
GAAGTGACAAAAATTACCGA+AGG - Chr1:56985801-56985820 MsG0180003124.01.T01:intron 35.0%
GCTTTGACCAAGAAAAACAA+AGG + Chr1:56983181-56983200 None:intergenic 35.0%
GGAACATCATCTGAAAATGA+GGG - Chr1:56982364-56982383 MsG0180003124.01.T01:intron 35.0%
GGGATGAATCAAGTTAGAAA+AGG + Chr1:56983593-56983612 None:intergenic 35.0%
GTGCACATAATTGTGGAAAA+TGG + Chr1:56982416-56982435 None:intergenic 35.0%
GTGGCTTAAGAAATTGAGTT+TGG - Chr1:56982983-56983002 MsG0180003124.01.T01:intron 35.0%
TCTCATATATGTCTTGCCTT+AGG + Chr1:56983632-56983651 None:intergenic 35.0%
TGAGTAGAGAAAGCGAAATT+AGG + Chr1:56982849-56982868 None:intergenic 35.0%
TGGTTTAATGCACTTTGATG+GGG - Chr1:56986019-56986038 MsG0180003124.01.T01:CDS 35.0%
TTCTTATAAATAGGACCGGA+GGG - Chr1:56983348-56983367 MsG0180003124.01.T01:intron 35.0%
TTTCTTATAAATAGGACCGG+AGG - Chr1:56983347-56983366 MsG0180003124.01.T01:intron 35.0%
! GACAACTCAAGAGAACTTTT+TGG - Chr1:56985931-56985950 MsG0180003124.01.T01:CDS 35.0%
! GCAATAAAAATAAAAGCGCG+TGG - Chr1:56985529-56985548 MsG0180003124.01.T01:CDS 35.0%
! GCAGAAAGTGTAATTCTCAA+TGG - Chr1:56983967-56983986 MsG0180003124.01.T01:intron 35.0%
! TTGAAAAGATTTAGGGTTCG+GGG - Chr1:56985010-56985029 MsG0180003124.01.T01:intron 35.0%
! TTTGGTGTGTGAAATTGTGA+AGG - Chr1:56985949-56985968 MsG0180003124.01.T01:CDS 35.0%
!! GCAAGTTGATCTCTTAATCA+TGG - Chr1:56984029-56984048 MsG0180003124.01.T01:intron 35.0%
!!! ACCAGTTTTCTAGTTGCATT+AGG - Chr1:56983518-56983537 MsG0180003124.01.T01:intron 35.0%
!!! ATATGTGTCGCGTCATTTTT+TGG - Chr1:56984884-56984903 MsG0180003124.01.T01:intron 35.0%
AACTTTATGACTGCCATGGA+AGG - Chr1:56982343-56982362 MsG0180003124.01.T01:CDS 40.0%
AATTCTCAATGGAGACCCAA+GGG - Chr1:56983978-56983997 MsG0180003124.01.T01:intron 40.0%
AATTTCTGACCTACTACCTC+CGG - Chr1:56983233-56983252 MsG0180003124.01.T01:intron 40.0%
ACCAATTCTCATTTGCCACT+TGG + Chr1:56985331-56985350 None:intergenic 40.0%
ATCAGCAGTTATTCTTTCGC+GGG - Chr1:56982786-56982805 MsG0180003124.01.T01:intron 40.0%
CAACTATCTAATACTCCCTC+CGG + Chr1:56983366-56983385 None:intergenic 40.0%
CAATAGGGTGCACATAATTG+TGG + Chr1:56982423-56982442 None:intergenic 40.0%
CACAATTATGTGCACCCTAT+TGG - Chr1:56982421-56982440 MsG0180003124.01.T01:intron 40.0%
CCTGACAAATCATTTCCCTT+GGG + Chr1:56983996-56984015 None:intergenic 40.0%
CGAAGGGACAAAATTCTGAA+GGG - Chr1:56985818-56985837 MsG0180003124.01.T01:intron 40.0%
CGTCAACACAAGCTAAATGA+GGG - Chr1:56985888-56985907 MsG0180003124.01.T01:CDS 40.0%
CTTCAACTTTATGACTGCCA+TGG - Chr1:56982339-56982358 MsG0180003124.01.T01:CDS 40.0%
CTTGGTCAAAGCCAGATAAT+GGG - Chr1:56983189-56983208 MsG0180003124.01.T01:intron 40.0%
GAAGCTTCTATGCTCTTAAG+TGG - Chr1:56982302-56982321 MsG0180003124.01.T01:CDS 40.0%
GATCAGCAGTTATTCTTTCG+CGG - Chr1:56982785-56982804 MsG0180003124.01.T01:intron 40.0%
GGAAAACTTAATGGAATGGG+TGG + Chr1:56983482-56983501 None:intergenic 40.0%
GGGGATTTGAATGAGATTGA+TGG - Chr1:56986038-56986057 MsG0180003124.01.T01:CDS 40.0%
GTTGTGGATTCAGTGAAGAA+AGG - Chr1:56982751-56982770 MsG0180003124.01.T01:intron 40.0%
TAACTGCTGATCTTTCTTGC+TGG + Chr1:56982777-56982796 None:intergenic 40.0%
TAATTCTCAATGGAGACCCA+AGG - Chr1:56983977-56983996 MsG0180003124.01.T01:intron 40.0%
TCGTCAACACAAGCTAAATG+AGG - Chr1:56985887-56985906 MsG0180003124.01.T01:CDS 40.0%
TCTCTTATAAATTGGACCGG+AGG + Chr1:56983252-56983271 None:intergenic 40.0%
TCTTGGTCAAAGCCAGATAA+TGG - Chr1:56983188-56983207 MsG0180003124.01.T01:intron 40.0%
TTCAGATGATGTTCCTTCCA+TGG + Chr1:56982359-56982378 None:intergenic 40.0%
! AGCTTTTTATCCAGCATCTG+TGG - Chr1:56982729-56982748 MsG0180003124.01.T01:intron 40.0%
! CCTTCAGAATTTTGTCCCTT+CGG + Chr1:56985820-56985839 None:intergenic 40.0%
! GTGCGTTGAATTGAGACTTT+TGG - Chr1:56982599-56982618 MsG0180003124.01.T01:intron 40.0%
! GTGGATTTTGTAGTGACGTT+GGG - Chr1:56985548-56985567 MsG0180003124.01.T01:CDS 40.0%
! TGAAAAGATTTAGGGTTCGG+GGG - Chr1:56985011-56985030 MsG0180003124.01.T01:intron 40.0%
!! GGGTTGGTTATATTTAGGGA+AGG - Chr1:56985031-56985050 MsG0180003124.01.T01:intron 40.0%
!! TCAATGGCTTGTTGGGATTA+GGG + Chr1:56983426-56983445 None:intergenic 40.0%
!! TCGGGGGTTGGTTATATTTA+GGG - Chr1:56985027-56985046 MsG0180003124.01.T01:intron 40.0%
!! TTCAATGGCTTGTTGGGATT+AGG + Chr1:56983427-56983446 None:intergenic 40.0%
!! TTCGGGGGTTGGTTATATTT+AGG - Chr1:56985026-56985045 MsG0180003124.01.T01:intron 40.0%
!!! GCGTCATTTTTTGGAGATCA+AGG - Chr1:56984893-56984912 MsG0180003124.01.T01:intron 40.0%
CAATTTCTACCCGAACTACG+AGG + Chr1:56985250-56985269 None:intergenic 45.0%
CCGAAGGGACAAAATTCTGA+AGG - Chr1:56985817-56985836 MsG0180003124.01.T01:intron 45.0%
CCGAAGGGACAGAAAAAAGA+GGG - Chr1:56985848-56985867 MsG0180003124.01.T01:intron 45.0%
CTGAAGGGACTTATTCCGAA+GGG - Chr1:56985833-56985852 MsG0180003124.01.T01:intron 45.0%
CTTAGTCGGACAATGTGATC+CGG + Chr1:56982563-56982582 None:intergenic 45.0%
GCAAATGTGCAAGGATAAGC+AGG - Chr1:56982813-56982832 MsG0180003124.01.T01:intron 45.0%
GCCAAGTGGCAAATGAGAAT+TGG - Chr1:56985327-56985346 MsG0180003124.01.T01:intron 45.0%
GCCTGACAAATCATTTCCCT+TGG + Chr1:56983997-56984016 None:intergenic 45.0%
GGATCAAAACCTCGTAGTTC+GGG - Chr1:56985238-56985257 MsG0180003124.01.T01:intron 45.0%
GGGATCAAAACCTCGTAGTT+CGG - Chr1:56985237-56985256 MsG0180003124.01.T01:intron 45.0%
GTGGCAATAGATGAGAGTGA+TGG - Chr1:56982280-56982299 MsG0180003124.01.T01:CDS 45.0%
TAAATTGGACCGGAGGTAGT+AGG + Chr1:56983245-56983264 None:intergenic 45.0%
TCCGAAGGGACAGAAAAAAG+AGG - Chr1:56985847-56985866 MsG0180003124.01.T01:intron 45.0%
TCTGAAGGGACTTATTCCGA+AGG - Chr1:56985832-56985851 MsG0180003124.01.T01:intron 45.0%
TTAGTCGGACAATGTGATCC+GGG + Chr1:56982562-56982581 None:intergenic 45.0%
TTATCCAGCATCTGTGGTTG+TGG - Chr1:56982735-56982754 MsG0180003124.01.T01:intron 45.0%
! CCCAAGGGAAATGATTTGTC+AGG - Chr1:56983993-56984012 MsG0180003124.01.T01:intron 45.0%
! CGTGGATTTTGTAGTGACGT+TGG - Chr1:56985547-56985566 MsG0180003124.01.T01:CDS 45.0%
!! GGTGGGTGTGATTTTGGAAA+GGG + Chr1:56983464-56983483 None:intergenic 45.0%
!! TACGAGGTTTTGATCCCTCA+CGG + Chr1:56985234-56985253 None:intergenic 45.0%
!!! CCCTCTTTTTTCTGTCCCTT+CGG + Chr1:56985851-56985870 None:intergenic 45.0%
!!! AAAAAATTCTAATTATTTTT+GGG + Chr1:56984330-56984349 None:intergenic 5.0%
!!! AAGAATAAAAATTTTAAATT+CGG + Chr1:56983068-56983087 None:intergenic 5.0%
!!! ATTTTAATTTTTTTAAAGAA+AGG - Chr1:56984960-56984979 MsG0180003124.01.T01:intron 5.0%
!!! TAAAAAATTCTAATTATTTT+TGG + Chr1:56984331-56984350 None:intergenic 5.0%
!!! TTTTAATTTTTTTAAAGAAA+GGG - Chr1:56984961-56984980 MsG0180003124.01.T01:intron 5.0%
!!! TTTTTTATTAATTTTGAAAA+AGG - Chr1:56985289-56985308 MsG0180003124.01.T01:intron 5.0%
ATGACCACGACGCACATAAG+TGG - Chr1:56982519-56982538 MsG0180003124.01.T01:intron 50.0%
CTCTTAATCATGGGTAGCCG+TGG - Chr1:56984039-56984058 MsG0180003124.01.T01:intron 50.0%
GAATCCACAACCACAGATGC+TGG + Chr1:56982742-56982761 None:intergenic 50.0%
TATGTGCACCCTATTGGACC+AGG - Chr1:56982427-56982446 MsG0180003124.01.T01:intron 50.0%
! AAGATTTAGGGTTCGGGGGT+TGG - Chr1:56985015-56985034 MsG0180003124.01.T01:intron 50.0%
! GGAATGGGTGGGTGTGATTT+TGG + Chr1:56983470-56983489 None:intergenic 50.0%
!! ACGAGGTTTTGATCCCTCAC+GGG + Chr1:56985233-56985252 None:intergenic 50.0%
!! CTAACCTTTTGAGTGTGCCC+AGG + Chr1:56984065-56984084 None:intergenic 50.0%
!! GGGTGGGTGTGATTTTGGAA+AGG + Chr1:56983465-56983484 None:intergenic 50.0%
ACCACGACGCACATAAGTGG+CGG - Chr1:56982522-56982541 MsG0180003124.01.T01:intron 55.0%
AGATACGAACCAGCACCACC+TGG + Chr1:56982448-56982467 None:intergenic 55.0%
CAGCACCACCTGGTCCAATA+GGG + Chr1:56982438-56982457 None:intergenic 55.0%
GCGGGCTTTGCAAATGTGCA+AGG - Chr1:56982804-56982823 MsG0180003124.01.T01:intron 55.0%
TTGATCCCTCACGGGTACGT+AGG + Chr1:56985225-56985244 None:intergenic 55.0%
! GCACATAAGTGGCGGAGTCA+GGG - Chr1:56982530-56982549 MsG0180003124.01.T01:intron 55.0%
!! TTTTGAGTGTGCCCAGGCCA+CGG + Chr1:56984059-56984078 None:intergenic 55.0%
AATCATGGGTAGCCGTGGCC+TGG - Chr1:56984044-56984063 MsG0180003124.01.T01:intron 60.0%
ATCATGGGTAGCCGTGGCCT+GGG - Chr1:56984045-56984064 MsG0180003124.01.T01:intron 60.0%
CCAGCACCACCTGGTCCAAT+AGG + Chr1:56982439-56982458 None:intergenic 60.0%
CCTATTGGACCAGGTGGTGC+TGG - Chr1:56982436-56982455 MsG0180003124.01.T01:intron 60.0%
CTCTGCCTACGTACCCGTGA+GGG - Chr1:56985217-56985236 MsG0180003124.01.T01:intron 60.0%
GTGCACCCTATTGGACCAGG+TGG - Chr1:56982430-56982449 MsG0180003124.01.T01:intron 60.0%
GTGGCCTGGGCACACTCAAA+AGG - Chr1:56984058-56984077 MsG0180003124.01.T01:intron 60.0%
TCCGCCACTTATGTGCGTCG+TGG + Chr1:56982526-56982545 None:intergenic 60.0%
! CGCACATAAGTGGCGGAGTC+AGG - Chr1:56982529-56982548 MsG0180003124.01.T01:intron 60.0%
CCTCACGGGTACGTAGGCAG+AGG + Chr1:56985219-56985238 None:intergenic 65.0%
CCTCTGCCTACGTACCCGTG+AGG - Chr1:56985216-56985235 MsG0180003124.01.T01:intron 65.0%
! GCGGAGTCAGGGTTCTGACC+CGG - Chr1:56982541-56982560 MsG0180003124.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 56982268 56986073 56982268 ID=MsG0180003124.01;Name=MsG0180003124.01
Chr1 mRNA 56982268 56986073 56982268 ID=MsG0180003124.01.T01;Parent=MsG0180003124.01;Name=MsG0180003124.01.T01;_AED=0.50;_eAED=0.60;_QI=0|0|0|1|1|1|4|0|171
Chr1 exon 56985884 56986073 56985884 ID=MsG0180003124.01.T01:exon:16074;Parent=MsG0180003124.01.T01
Chr1 exon 56985507 56985610 56985507 ID=MsG0180003124.01.T01:exon:16073;Parent=MsG0180003124.01.T01
Chr1 exon 56984262 56984380 56984262 ID=MsG0180003124.01.T01:exon:16072;Parent=MsG0180003124.01.T01
Chr1 exon 56982268 56982370 56982268 ID=MsG0180003124.01.T01:exon:16071;Parent=MsG0180003124.01.T01
Chr1 CDS 56985884 56986073 56985884 ID=MsG0180003124.01.T01:cds;Parent=MsG0180003124.01.T01
Chr1 CDS 56985507 56985610 56985507 ID=MsG0180003124.01.T01:cds;Parent=MsG0180003124.01.T01
Chr1 CDS 56984262 56984380 56984262 ID=MsG0180003124.01.T01:cds;Parent=MsG0180003124.01.T01
Chr1 CDS 56982268 56982370 56982268 ID=MsG0180003124.01.T01:cds;Parent=MsG0180003124.01.T01
Gene Sequence

>MsG0180003124.01.T01

ATGAAGGTGATGGTGGCAATAGATGAGAGTGATGGAAGCTTCTATGCTCTTAAGTGGGCCCTTGATAATCTCTTCAACTTTATGACTGCCATGGAAGGAACATCATCTGAAAATGAGGGAATGGTGTTTCTTGTTCATGTTGAACCCATTTTCCACAATTATGTGCACCCTATTGGACCAGGTGGTGCTGCTTTTTATCCAGCATCTGTGGTTGTGGATTCAGTGAAGAAAGGCCAGCAAGAAAGATCAGCAGTTATTCTTTCGCGGGCTTTGCAAATGTGCAAGGATAAGCAGGTCAAAGCAGAAAGTGTAATTCTCAATGGAGACCCAAGGGAAATGATTTGTCAGGCTTCAGAACAAATGCAAGTTGATCTCTTAATCATGGGTAGCCGTGGCCTGGGCACACTCAAAAGGAAGAGAGCTCTATTTATAGGTTCTAAGGTTGATGAAAAATGGAAAATTGGTTTAATGCACTTTGATGGGGATTTGAATGAGATTGATGGTTTTGGAATGTGA
Protein sequence

>MsG0180003124.01.T01

MKVMVAIDESDGSFYALKWALDNLFNFMTAMEGTSSENEGMVFLVHVEPIFHNYVHPIGPGGAAFYPASVVVDSVKKGQQERSAVILSRALQMCKDKQVKAESVILNGDPREMICQASEQMQVDLLIMGSRGLGTLKRKRALFIGSKVDEKWKIGLMHFDGDLNEIDGFGM*