Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043114.01.T01 | XP_013444778.1 | 99.123 | 114 | 1 | 0 | 1 | 114 | 36 | 149 | 3.90E-75 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043114.01.T01 | O03982 | 50 | 142 | 43 | 3 | 1 | 114 | 36 | 177 | 2.49E-24 | 94 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043114.01.T01 | A0A072TPN8 | 99.123 | 114 | 1 | 0 | 1 | 114 | 36 | 149 | 1.86e-75 | 229 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049016.01 | MsG0880043114.01 | 0.829555 | 4.822307e-55 | 4.952044e-52 |
| MsG0180003451.01 | MsG0880043114.01 | 0.811485 | 6.839801e-51 | 4.239196e-48 |
| MsG0180004937.01 | MsG0880043114.01 | 0.846942 | 1.581752e-59 | 2.766952e-56 |
| MsG0180005568.01 | MsG0880043114.01 | 0.818567 | 1.832267e-52 | 1.376376e-49 |
| MsG0780041618.01 | MsG0880043114.01 | 0.801011 | 1.101302e-48 | 5.203347e-46 |
| MsG0880042021.01 | MsG0880043114.01 | 0.811524 | 6.707715e-51 | 4.161584e-48 |
| MsG0880042921.01 | MsG0880043114.01 | 0.810477 | 1.130708e-50 | 6.822740e-48 |
| MsG0880043114.01 | MsG0880044558.01 | 0.803787 | 2.950078e-49 | 1.495887e-46 |
| MsG0880043114.01 | MsG0880045283.01 | 0.823143 | 1.623232e-53 | 1.386165e-50 |
| MsG0880043114.01 | MsG0880046024.01 | 0.807181 | 5.730226e-50 | 3.170416e-47 |
| MsG0880043114.01 | MsG0880046288.01 | 0.841292 | 5.190940e-58 | 7.592033e-55 |
| MsG0880043114.01 | MsG0880046456.01 | 0.819054 | 1.420270e-52 | 1.081400e-49 |
| MsG0880043114.01 | MsG0880046574.01 | 0.839955 | 1.162820e-57 | 1.631823e-54 |
| MsG0880043114.01 | MsG0880046856.01 | 0.818214 | 2.203255e-52 | 1.638990e-49 |
| MsG0880043114.01 | MsG0880046968.01 | 0.805339 | 1.400304e-49 | 7.389449e-47 |
| MsG0880043114.01 | MsG0880046969.01 | 0.807038 | 6.142254e-50 | 3.385775e-47 |
| MsG0880043114.01 | MsG0880047660.01 | 0.801096 | 1.057754e-48 | 5.008431e-46 |
| MsG0280011067.01 | MsG0880043114.01 | 0.815520 | 8.865139e-52 | 6.126050e-49 |
| MsG0680034724.01 | MsG0880043114.01 | 0.800942 | 1.137580e-48 | 5.365208e-46 |
| MsG0680034729.01 | MsG0880043114.01 | 0.810417 | 1.164882e-50 | 7.017616e-48 |
| MsG0680035612.01 | MsG0880043114.01 | 0.807302 | 5.400840e-50 | 2.997688e-47 |
| MsG0480021420.01 | MsG0880043114.01 | 0.826408 | 2.758109e-54 | 2.584492e-51 |
| MsG0480021999.01 | MsG0880043114.01 | 0.807183 | 5.723245e-50 | 3.166730e-47 |
| MsG0480023371.01 | MsG0880043114.01 | 0.804202 | 2.418696e-49 | 1.239511e-46 |
| MsG0480023422.01 | MsG0880043114.01 | 0.801723 | 7.869407e-49 | 3.785184e-46 |
| MsG0480023505.01 | MsG0880043114.01 | 0.809094 | 2.242308e-50 | 1.304513e-47 |
| MsG0480023708.01 | MsG0880043114.01 | 0.809190 | 2.138885e-50 | 1.247557e-47 |
| MsG0380015545.01 | MsG0880043114.01 | 0.816938 | 4.271015e-52 | 3.068316e-49 |
| MsG0380016074.01 | MsG0880043114.01 | 0.809755 | 1.617327e-50 | 9.576633e-48 |
| MsG0380016723.01 | MsG0880043114.01 | 0.845262 | 4.532606e-59 | 7.511226e-56 |
| MsG0380017056.01 | MsG0880043114.01 | 0.808972 | 2.381237e-50 | 1.380769e-47 |
| MsG0480018089.01 | MsG0880043114.01 | 0.808914 | 2.450238e-50 | 1.418540e-47 |
| MsG0480018323.01 | MsG0880043114.01 | 0.809318 | 2.007512e-50 | 1.174901e-47 |
| MsG0580027098.01 | MsG0880043114.01 | 0.802481 | 5.497098e-49 | 2.695661e-46 |
| MsG0580028853.01 | MsG0880043114.01 | 0.809273 | 2.052705e-50 | 1.199934e-47 |
| MsG0580029706.01 | MsG0880043114.01 | 0.819649 | 1.039780e-52 | 8.048584e-50 |
| MsG0580029820.01 | MsG0880043114.01 | 0.808904 | 2.462740e-50 | 1.425374e-47 |
| MsG0280007600.01 | MsG0880043114.01 | 0.823657 | 1.230704e-53 | 1.066374e-50 |
| MsG0280008233.01 | MsG0880043114.01 | 0.804520 | 2.076753e-49 | 1.072998e-46 |
| MsG0280008939.01 | MsG0880043114.01 | 0.817259 | 3.618191e-52 | 2.621942e-49 |
| MsG0280008966.01 | MsG0880043114.01 | 0.838240 | 3.233842e-57 | 4.304761e-54 |
| MsG0280010198.01 | MsG0880043114.01 | 0.812854 | 3.438875e-51 | 2.210418e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043114.01.T01 | MTR_8g028615 | 99.123 | 114 | 1 | 0 | 1 | 114 | 36 | 149 | 4.73e-79 | 229 |
| MsG0880043114.01.T01 | MTR_8g098915 | 52.632 | 114 | 32 | 1 | 1 | 114 | 36 | 127 | 1.41e-33 | 113 |
| MsG0880043114.01.T01 | MTR_8g098915 | 53.509 | 114 | 31 | 1 | 1 | 114 | 17 | 108 | 2.85e-33 | 112 |
| MsG0880043114.01.T01 | MTR_7g079110 | 46.715 | 137 | 50 | 1 | 1 | 114 | 36 | 172 | 6.41e-28 | 100 |
| MsG0880043114.01.T01 | MTR_6g051690 | 35.965 | 114 | 51 | 2 | 1 | 114 | 36 | 127 | 8.06e-12 | 58.2 |
| MsG0880043114.01.T01 | MTR_6g049280 | 38.596 | 114 | 45 | 2 | 1 | 114 | 36 | 124 | 2.88e-11 | 56.6 |
| MsG0880043114.01.T01 | MTR_1g099800 | 39.316 | 117 | 50 | 2 | 1 | 113 | 36 | 135 | 6.04e-11 | 55.8 |
| MsG0880043114.01.T01 | MTR_6g051680 | 65.714 | 35 | 12 | 0 | 1 | 35 | 36 | 70 | 6.16e-11 | 55.5 |
| MsG0880043114.01.T01 | MTR_6g049280 | 38.596 | 114 | 45 | 2 | 1 | 114 | 66 | 154 | 8.12e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043114.01.T01 | AT1G01490 | 50.000 | 142 | 43 | 3 | 1 | 114 | 63 | 204 | 1.57e-25 | 95.5 |
| MsG0880043114.01.T01 | AT1G01490 | 50.000 | 142 | 43 | 3 | 1 | 114 | 36 | 177 | 2.54e-25 | 94.0 |
| MsG0880043114.01.T01 | AT1G01490 | 50.000 | 142 | 43 | 3 | 1 | 114 | 36 | 177 | 2.54e-25 | 94.0 |
| MsG0880043114.01.T01 | AT1G01490 | 50.000 | 142 | 43 | 3 | 1 | 114 | 3 | 144 | 5.36e-25 | 92.4 |
Find 25 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTGCTAACCACATTTAC+TGG | 0.205824 | 8:+19255350 | None:intergenic |
| TTAGCAAATTAAGAAAATTC+TGG | 0.328417 | 8:-19255337 | MsG0880043114.01.T01:CDS |
| TTCCTAATCCATTCTTGAAT+AGG | 0.370743 | 8:+19255152 | None:intergenic |
| AAAGAAAGAAGAAGGAAAGA+AGG | 0.374548 | 8:-19255243 | MsG0880043114.01.T01:CDS |
| GAGAAGAAACTGACAGTGAT+AGG | 0.416746 | 8:-19255383 | MsG0880043114.01.T01:CDS |
| GAAGAAGGAAAGAAAGAAGA+AGG | 0.419026 | 8:-19255221 | MsG0880043114.01.T01:CDS |
| ATCCTATTCAAGAATGGATT+AGG | 0.419464 | 8:-19255154 | MsG0880043114.01.T01:CDS |
| GAAGAAGGAAAGAAGGAAGA+AGG | 0.432303 | 8:-19255236 | MsG0880043114.01.T01:CDS |
| TCTTGAATAGGATCTGGTGG+TGG | 0.433366 | 8:+19255164 | None:intergenic |
| GATGGAGAAAAGAAAGAAGA+AGG | 0.445221 | 8:-19255251 | MsG0880043114.01.T01:CDS |
| AAAGAAGAAGCCAAGAAAGA+TGG | 0.450668 | 8:-19255269 | MsG0880043114.01.T01:CDS |
| CTTGAATAGGATCTGGTGGT+GGG | 0.454168 | 8:+19255165 | None:intergenic |
| ATCCATTCTTGAATAGGATC+TGG | 0.462680 | 8:+19255158 | None:intergenic |
| TAACAAATGACACATGCATT+GGG | 0.466318 | 8:+19255068 | None:intergenic |
| TGAATAGGATCTGGTGGTGG+GGG | 0.487070 | 8:+19255167 | None:intergenic |
| TTAACAAATGACACATGCAT+TGG | 0.497832 | 8:+19255067 | None:intergenic |
| CACCAGATCCTATTCAAGAA+TGG | 0.515760 | 8:-19255160 | MsG0880043114.01.T01:CDS |
| ATGGTAATGTGTTGTCATGT+AGG | 0.522412 | 8:+19255112 | None:intergenic |
| TTGAATAGGATCTGGTGGTG+GGG | 0.549295 | 8:+19255166 | None:intergenic |
| TCTCTTCCATGCTTTGAACA+TGG | 0.559376 | 8:+19255093 | None:intergenic |
| TGGTAATGTGTTGTCATGTA+GGG | 0.575883 | 8:+19255113 | None:intergenic |
| AACAGTAGATCCAGTAAATG+TGG | 0.594091 | 8:-19255360 | MsG0880043114.01.T01:CDS |
| ACATTACCATGTTCAAAGCA+TGG | 0.617021 | 8:-19255099 | MsG0880043114.01.T01:CDS |
| CATTCTTGAATAGGATCTGG+TGG | 0.618384 | 8:+19255161 | None:intergenic |
| CATACAGAAATAGTAGCAGT+AGG | 0.633124 | 8:-19255314 | MsG0880043114.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGCAAATTAAGAAAATTC+TGG | - | Chr8:19255119-19255138 | MsG0880043114.01.T01:CDS | 20.0% |
| AAAGAAAGAAGAAGGAAAGA+AGG | - | Chr8:19255213-19255232 | MsG0880043114.01.T01:CDS | 30.0% | |
| TAACAAATGACACATGCATT+GGG | + | Chr8:19255391-19255410 | None:intergenic | 30.0% | |
| TTAACAAATGACACATGCAT+TGG | + | Chr8:19255392-19255411 | None:intergenic | 30.0% | |
| TTCCTAATCCATTCTTGAAT+AGG | + | Chr8:19255307-19255326 | None:intergenic | 30.0% | |
| ! | ATCCTATTCAAGAATGGATT+AGG | - | Chr8:19255302-19255321 | MsG0880043114.01.T01:CDS | 30.0% |
| ! | TTTCTTTTCTCCATCTTTCT+TGG | + | Chr8:19255200-19255219 | None:intergenic | 30.0% |
| !! | AATTTGCTAACCACATTTAC+TGG | + | Chr8:19255109-19255128 | None:intergenic | 30.0% |
| AAAGAAGAAGCCAAGAAAGA+TGG | - | Chr8:19255187-19255206 | MsG0880043114.01.T01:CDS | 35.0% | |
| AACAGTAGATCCAGTAAATG+TGG | - | Chr8:19255096-19255115 | MsG0880043114.01.T01:CDS | 35.0% | |
| ACATTACCATGTTCAAAGCA+TGG | - | Chr8:19255357-19255376 | MsG0880043114.01.T01:CDS | 35.0% | |
| ATCCATTCTTGAATAGGATC+TGG | + | Chr8:19255301-19255320 | None:intergenic | 35.0% | |
| CATACAGAAATAGTAGCAGT+AGG | - | Chr8:19255142-19255161 | MsG0880043114.01.T01:CDS | 35.0% | |
| GAAGAAGGAAAGAAAGAAGA+AGG | - | Chr8:19255235-19255254 | MsG0880043114.01.T01:CDS | 35.0% | |
| GATGGAGAAAAGAAAGAAGA+AGG | - | Chr8:19255205-19255224 | MsG0880043114.01.T01:CDS | 35.0% | |
| ! | ATGGTAATGTGTTGTCATGT+AGG | + | Chr8:19255347-19255366 | None:intergenic | 35.0% |
| ! | TGGTAATGTGTTGTCATGTA+GGG | + | Chr8:19255346-19255365 | None:intergenic | 35.0% |
| ! | TTGGCTTCTTCTTTCTTTTC+AGG | + | Chr8:19255181-19255200 | None:intergenic | 35.0% |
| !! | TTCTTTCTTTTCAGGCTCTT+TGG | + | Chr8:19255173-19255192 | None:intergenic | 35.0% |
| CACCAGATCCTATTCAAGAA+TGG | - | Chr8:19255296-19255315 | MsG0880043114.01.T01:CDS | 40.0% | |
| CATTCTTGAATAGGATCTGG+TGG | + | Chr8:19255298-19255317 | None:intergenic | 40.0% | |
| GAAGAAGGAAAGAAGGAAGA+AGG | - | Chr8:19255220-19255239 | MsG0880043114.01.T01:CDS | 40.0% | |
| GAGAAGAAACTGACAGTGAT+AGG | - | Chr8:19255073-19255092 | MsG0880043114.01.T01:CDS | 40.0% | |
| ! | TCTCTTCCATGCTTTGAACA+TGG | + | Chr8:19255366-19255385 | None:intergenic | 40.0% |
| CTTGAATAGGATCTGGTGGT+GGG | + | Chr8:19255294-19255313 | None:intergenic | 45.0% | |
| TCTTGAATAGGATCTGGTGG+TGG | + | Chr8:19255295-19255314 | None:intergenic | 45.0% | |
| ! | TTGAATAGGATCTGGTGGTG+GGG | + | Chr8:19255293-19255312 | None:intergenic | 45.0% |
| !! | TTCTTTTCAGGCTCTTTGGC+TGG | + | Chr8:19255169-19255188 | None:intergenic | 45.0% |
| !! | TGAATAGGATCTGGTGGTGG+GGG | + | Chr8:19255292-19255311 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 19255067 | 19255411 | 19255067 | ID=MsG0880043114.01;Name=MsG0880043114.01 |
| Chr8 | mRNA | 19255067 | 19255411 | 19255067 | ID=MsG0880043114.01.T01;Parent=MsG0880043114.01;Name=MsG0880043114.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|114 |
| Chr8 | exon | 19255067 | 19255411 | 19255067 | ID=MsG0880043114.01.T01:exon:5053;Parent=MsG0880043114.01.T01 |
| Chr8 | CDS | 19255067 | 19255411 | 19255067 | ID=MsG0880043114.01.T01:cds;Parent=MsG0880043114.01.T01 |
| Gene Sequence |
| Protein sequence |
