AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046456.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046456.01.T01 MTR_0140s0030 97.573 206 5 0 1 206 117 322 1.57e-146 411
MsG0880046456.01.T01 MTR_0489s0020 97.573 206 5 0 1 206 117 322 1.57e-146 411
MsG0880046456.01.T01 MTR_3g039990 38.028 213 88 9 4 206 121 299 1.46e-26 103
MsG0880046456.01.T01 MTR_6g012180 35.533 197 108 7 11 205 130 309 9.80e-21 88.2
MsG0880046456.01.T01 MTR_7g010210 31.878 229 108 10 1 208 116 317 1.56e-17 79.7
MsG0880046456.01.T01 MTR_7g010210 40.678 118 43 4 1 105 116 219 9.50e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046456.01.T01 AT1G74650 32.474 194 98 7 23 208 75 243 2.92e-14 69.7
MsG0880046456.01.T01 AT3G28910 30.366 191 87 5 23 197 73 233 3.56e-14 69.3
MsG0880046456.01.T01 AT1G74650 32.474 194 98 7 23 208 80 248 3.83e-14 69.3
MsG0880046456.01.T01 AT3G28910 30.366 191 87 5 23 197 149 309 1.06e-13 68.9
MsG0880046456.01.T01 AT1G74650 32.812 192 96 7 23 206 156 322 1.58e-13 68.6
MsG0880046456.01.T01 AT3G47600 34.444 180 92 8 23 194 147 308 3.46e-13 67.4
MsG0880046456.01.T01 AT3G47600 34.444 180 92 8 23 194 153 314 4.86e-13 67.0
MsG0880046456.01.T01 AT5G62470 34.434 212 87 9 23 200 146 339 1.22e-11 63.2
MsG0880046456.01.T01 AT5G62470 34.434 212 87 9 23 200 145 338 1.25e-11 63.2

Find 45 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATTATGCATCTCGATTT+AGG 0.240478 8:+73584632 MsG0880046456.01.T01:CDS
GATTAATATTAAAGCAAATA+TGG 0.292292 8:+73584726 MsG0880046456.01.T01:CDS
TGAAAACATTTCTAGATTGA+TGG 0.297412 8:+73584474 MsG0880046456.01.T01:CDS
AGCCTCAGATAAGGCTTGTT+TGG 0.361155 8:-73584323 None:intergenic
CTTTCCCATTGACCTTTATA+CGG 0.370223 8:-73584274 None:intergenic
TCGAGATGCATAATCAGATT+TGG 0.373847 8:-73584626 None:intergenic
CGTTCGCAGCTGCCGTATAA+AGG 0.376269 8:+73584262 MsG0880046456.01.T01:CDS
TTTGTTTCAGCTGATGAGTT+AGG 0.376364 8:-73584514 None:intergenic
CATATGGAGATGGAATATTC+AGG 0.382987 8:-73584441 None:intergenic
AATCAGATTTGGTTGAGTTA+AGG 0.409124 8:-73584615 None:intergenic
GATGAGGGTTTCATCTGATC+AGG 0.419961 8:-73584385 None:intergenic
TAATATTAATCCTTTCCTCT+TGG 0.421047 8:-73584714 None:intergenic
AGCTTATAACATGTCATTAG+AGG 0.422579 8:+73584824 MsG0880046456.01.T01:CDS
GACATGTTATAAGCTCTTCA+TGG 0.424640 8:-73584816 None:intergenic
GGAGATGGAATATTCAGGTT+TGG 0.447433 8:-73584436 None:intergenic
TATTAATCCTTTCCTCTTGG+TGG 0.453369 8:-73584711 None:intergenic
AGCTGCCGTATAAAGGTCAA+TGG 0.472504 8:+73584269 MsG0880046456.01.T01:CDS
ATCGATATTCAGCAACATGC+AGG 0.473325 8:+73584543 MsG0880046456.01.T01:CDS
TTTCTAGATTGATGGAAACT+TGG 0.473758 8:+73584482 MsG0880046456.01.T01:CDS
GGCAAAGTACCAAGGGTTGT+AGG 0.474388 8:-73584364 None:intergenic
GGGTTGTAGGGTTATGTTGA+AGG 0.484469 8:-73584351 None:intergenic
GATGGAATATTCAGGTTTGG+TGG 0.513419 8:-73584433 None:intergenic
TATTAAAGCAAATATGGAAA+CGG 0.514444 8:+73584732 MsG0880046456.01.T01:CDS
GCTACAAACAGATATTCATA+TGG 0.516194 8:+73584300 MsG0880046456.01.T01:CDS
CAACATAACCCTACAACCCT+TGG 0.522290 8:+73584355 MsG0880046456.01.T01:CDS
CTTCAACTTGAACACAAAAG+AGG 0.524145 8:+73584678 MsG0880046456.01.T01:CDS
TTTCCTCTTGGTGGAAGAAG+AGG 0.526621 8:-73584702 None:intergenic
GCAAAGTACCAAGGGTTGTA+GGG 0.526670 8:-73584363 None:intergenic
ATAAAGGTCAATGGGAAAGA+AGG 0.529786 8:+73584278 MsG0880046456.01.T01:CDS
TCTTCTTCCACCAAGAGGAA+AGG 0.547130 8:+73584704 MsG0880046456.01.T01:CDS
TAGCTTGATGCATATGGAGA+TGG 0.555943 8:-73584451 None:intergenic
GCTGCCGTATAAAGGTCAAT+GGG 0.556660 8:+73584270 MsG0880046456.01.T01:CDS
GATCAAAGTAGTAACGATGA+AGG 0.558829 8:+73584223 MsG0880046456.01.T01:CDS
CGATTTAGGTCAACTCATGA+AGG 0.562437 8:+73584646 MsG0880046456.01.T01:CDS
TTCAACTTGAACACAAAAGA+GGG 0.567559 8:+73584679 MsG0880046456.01.T01:CDS
GGTTGTAGGGTTATGTTGAA+GGG 0.574998 8:-73584350 None:intergenic
AAGGGACAAAGCCTCAGATA+AGG 0.576279 8:-73584332 None:intergenic
CAGGGATCAAGTTGTAGTGA+AGG 0.587215 8:+73584562 MsG0880046456.01.T01:CDS
TCGATATTCAGCAACATGCA+GGG 0.617549 8:+73584544 MsG0880046456.01.T01:CDS
CTGATCAGGCAAAGTACCAA+GGG 0.619842 8:-73584371 None:intergenic
TCTGATCAGGCAAAGTACCA+AGG 0.632255 8:-73584372 None:intergenic
AGCACAACACAAGATCATCA+TGG 0.642061 8:+73584592 MsG0880046456.01.T01:CDS
GGGCCTCTTCTTCCACCAAG+AGG 0.657998 8:+73584699 MsG0880046456.01.T01:CDS
GGCCAAACAAGCCTTATCTG+AGG 0.663946 8:+73584321 MsG0880046456.01.T01:CDS
CATGTTGCTGAATATCGATG+AGG 0.741627 8:-73584539 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTAATATTAAAGCAAATA+TGG + Chr8:73584726-73584745 MsG0880046456.01.T01:CDS 15.0%
!! TATTAAAGCAAATATGGAAA+CGG + Chr8:73584732-73584751 MsG0880046456.01.T01:CDS 20.0%
! TAATATTAATCCTTTCCTCT+TGG - Chr8:73584717-73584736 None:intergenic 25.0%
!! TGAAAACATTTCTAGATTGA+TGG + Chr8:73584474-73584493 MsG0880046456.01.T01:CDS 25.0%
!!! AAAAATGGCTTTTTGAAGAT+GGG + Chr8:73584776-73584795 MsG0880046456.01.T01:CDS 25.0%
AGAATGAGAAAAACTTGATG+AGG - Chr8:73584404-73584423 None:intergenic 30.0%
CTCTTACTTTGCTTGAAAAA+TGG + Chr8:73584761-73584780 MsG0880046456.01.T01:CDS 30.0%
GAATGAGAAAAACTTGATGA+GGG - Chr8:73584403-73584422 None:intergenic 30.0%
GCTACAAACAGATATTCATA+TGG + Chr8:73584300-73584319 MsG0880046456.01.T01:CDS 30.0%
TTCAACTTGAACACAAAAGA+GGG + Chr8:73584679-73584698 MsG0880046456.01.T01:CDS 30.0%
TTTCTAGATTGATGGAAACT+TGG + Chr8:73584482-73584501 MsG0880046456.01.T01:CDS 30.0%
! AATCAGATTTGGTTGAGTTA+AGG - Chr8:73584618-73584637 None:intergenic 30.0%
! TTTTCATAGCTTGATGCATA+TGG - Chr8:73584460-73584479 None:intergenic 30.0%
!! AAAATGGCTTTTTGAAGATG+GGG + Chr8:73584777-73584796 MsG0880046456.01.T01:CDS 30.0%
!! GAAAAATGGCTTTTTGAAGA+TGG + Chr8:73584775-73584794 MsG0880046456.01.T01:CDS 30.0%
!!! CATTTTTCAAGCAAAGTAAG+AGG - Chr8:73584763-73584782 None:intergenic 30.0%
ATAAAGGTCAATGGGAAAGA+AGG + Chr8:73584278-73584297 MsG0880046456.01.T01:CDS 35.0%
CATATGGAGATGGAATATTC+AGG - Chr8:73584444-73584463 None:intergenic 35.0%
CTGATTATGCATCTCGATTT+AGG + Chr8:73584632-73584651 MsG0880046456.01.T01:CDS 35.0%
CTTCAACTTGAACACAAAAG+AGG + Chr8:73584678-73584697 MsG0880046456.01.T01:CDS 35.0%
CTTTCCCATTGACCTTTATA+CGG - Chr8:73584277-73584296 None:intergenic 35.0%
GACATGTTATAAGCTCTTCA+TGG - Chr8:73584819-73584838 None:intergenic 35.0%
GATCAAAGTAGTAACGATGA+AGG + Chr8:73584223-73584242 MsG0880046456.01.T01:CDS 35.0%
TATTAATCCTTTCCTCTTGG+TGG - Chr8:73584714-73584733 None:intergenic 35.0%
TCGAGATGCATAATCAGATT+TGG - Chr8:73584629-73584648 None:intergenic 35.0%
TTTGTTTCAGCTGATGAGTT+AGG - Chr8:73584517-73584536 None:intergenic 35.0%
!! AAATGGCTTTTTGAAGATGG+GGG + Chr8:73584778-73584797 MsG0880046456.01.T01:CDS 35.0%
AGCACAACACAAGATCATCA+TGG + Chr8:73584592-73584611 MsG0880046456.01.T01:CDS 40.0%
ATCGATATTCAGCAACATGC+AGG + Chr8:73584543-73584562 MsG0880046456.01.T01:CDS 40.0%
CATGTTGCTGAATATCGATG+AGG - Chr8:73584542-73584561 None:intergenic 40.0%
CGATTTAGGTCAACTCATGA+AGG + Chr8:73584646-73584665 MsG0880046456.01.T01:CDS 40.0%
GGTTGTAGGGTTATGTTGAA+GGG - Chr8:73584353-73584372 None:intergenic 40.0%
TAGCTTGATGCATATGGAGA+TGG - Chr8:73584454-73584473 None:intergenic 40.0%
TCGATATTCAGCAACATGCA+GGG + Chr8:73584544-73584563 MsG0880046456.01.T01:CDS 40.0%
! GATGGAATATTCAGGTTTGG+TGG - Chr8:73584436-73584455 None:intergenic 40.0%
! GGAGATGGAATATTCAGGTT+TGG - Chr8:73584439-73584458 None:intergenic 40.0%
AAGGGACAAAGCCTCAGATA+AGG - Chr8:73584335-73584354 None:intergenic 45.0%
AGCTGCCGTATAAAGGTCAA+TGG + Chr8:73584269-73584288 MsG0880046456.01.T01:CDS 45.0%
CAACATAACCCTACAACCCT+TGG + Chr8:73584355-73584374 MsG0880046456.01.T01:CDS 45.0%
GATGAGGGTTTCATCTGATC+AGG - Chr8:73584388-73584407 None:intergenic 45.0%
GCTGCCGTATAAAGGTCAAT+GGG + Chr8:73584270-73584289 MsG0880046456.01.T01:CDS 45.0%
GGGTTGTAGGGTTATGTTGA+AGG - Chr8:73584354-73584373 None:intergenic 45.0%
TCTTCTTCCACCAAGAGGAA+AGG + Chr8:73584704-73584723 MsG0880046456.01.T01:CDS 45.0%
TTTCCTCTTGGTGGAAGAAG+AGG - Chr8:73584705-73584724 None:intergenic 45.0%
! AGCCTCAGATAAGGCTTGTT+TGG - Chr8:73584326-73584345 None:intergenic 45.0%
! CAGGGATCAAGTTGTAGTGA+AGG + Chr8:73584562-73584581 MsG0880046456.01.T01:CDS 45.0%
! CTGATCAGGCAAAGTACCAA+GGG - Chr8:73584374-73584393 None:intergenic 45.0%
! GCAAAGTACCAAGGGTTGTA+GGG - Chr8:73584366-73584385 None:intergenic 45.0%
! TCTGATCAGGCAAAGTACCA+AGG - Chr8:73584375-73584394 None:intergenic 45.0%
! GGCAAAGTACCAAGGGTTGT+AGG - Chr8:73584367-73584386 None:intergenic 50.0%
! GGCCAAACAAGCCTTATCTG+AGG + Chr8:73584321-73584340 MsG0880046456.01.T01:CDS 50.0%
CGTTCGCAGCTGCCGTATAA+AGG + Chr8:73584262-73584281 MsG0880046456.01.T01:CDS 55.0%
GGGCCTCTTCTTCCACCAAG+AGG + Chr8:73584699-73584718 MsG0880046456.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 73584214 73584843 73584214 ID=MsG0880046456.01;Name=MsG0880046456.01
Chr8 mRNA 73584214 73584843 73584214 ID=MsG0880046456.01.T01;Parent=MsG0880046456.01;Name=MsG0880046456.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|209
Chr8 exon 73584214 73584843 73584214 ID=MsG0880046456.01.T01:exon:4052;Parent=MsG0880046456.01.T01
Chr8 CDS 73584214 73584843 73584214 ID=MsG0880046456.01.T01:cds;Parent=MsG0880046456.01.T01
Gene Sequence

>MsG0880046456.01.T01

ATGAACAAAGATCAAAGTAGTAACGATGAAGGTGTTGATCAAGAATCTCGTTCGCAGCTGCCGTATAAAGGTCAATGGGAAAGAAGGCTACAAACAGATATTCATATGGCCAAACAAGCCTTATCTGAGGCTTTGTCCCTTCAACATAACCCTACAACCCTTGGTACTTTGCCTGATCAGATGAAACCCTCATCAAGTTTTTCTCATTCTCATGAACATCCACCAAACCTGAATATTCCATCTCCATATGCATCAAGCTATGAAAACATTTCTAGATTGATGGAAACTTGGATGAAATCACCTAACTCATCAGCTGAAACAAATTCCTCATCGATATTCAGCAACATGCAGGGATCAAGTTGTAGTGAAGGAGCACAAAGCACAACACAAGATCATCATGGCCTTAACTCAACCAAATCTGATTATGCATCTCGATTTAGGTCAACTCATGAAGGAAACAACAGCTTCAACTTGAACACAAAAGAGGGCCTCTTCTTCCACCAAGAGGAAAGGATTAATATTAAAGCAAATATGGAAACGGATGTTCCTCTTACTTTGCTTGAAAAATGGCTTTTTGAAGATGGGGGAGCAAGTCATGAGTGCCATGAAGAGCTTATAACATGTCATTAG

Protein sequence

>MsG0880046456.01.T01

MNKDQSSNDEGVDQESRSQLPYKGQWERRLQTDIHMAKQALSEALSLQHNPTTLGTLPDQMKPSSSFSHSHEHPPNLNIPSPYASSYENISRLMETWMKSPNSSAETNSSSIFSNMQGSSCSEGAQSTTQDHHGLNSTKSDYASRFRSTHEGNNSFNLNTKEGLFFHQEERINIKANMETDVPLTLLEKWLFEDGGASHECHEELITCH*