AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0028440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0028440 MtrunA17_Chr1g0184931 95.712 723 29 2 1 722 1 722 0.0 1374
Msa0028440 MtrunA17_Chr7g0260511 49.662 739 255 30 12 722 4 653 1.23e-179 527
Msa0028440 MtrunA17_Chr7g0219501 29.104 268 136 13 492 713 667 926 6.74e-14 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0028440 AT3G63430.1 35.905 713 270 26 21 722 4 540 1.39e-107 338
Msa0028440 AT3G63430.2 37.002 627 221 22 103 722 49 508 2.27e-101 321
Msa0028440 AT5G15580.1 28.223 287 113 12 501 706 639 913 2.04e-14 77.8

Find 209 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATAATGTTAATTCTTA+TGG 0.170803 1_1:-53357630 Msa0028440:CDS
TTAATGACCATGACAAAATT+AGG 0.176366 1_1:-53355437 Msa0028440:three_prime_UTR
TAACCGTTGGACTTCAATTT+TGG 0.196054 1_1:+53357738 None:intergenic
TTAGAGGCTCTGCAGCTTAA+AGG 0.226674 1_1:-53357387 Msa0028440:CDS
AGGAAGAGGGAAGCAGAATA+AGG 0.226928 1_1:-53357565 Msa0028440:CDS
CAGTGTTATTGTTAGATTAA+TGG 0.236552 1_1:-53357847 Msa0028440:CDS
TCTTATGGTAACATTGATAA+TGG 0.256110 1_1:-53357615 Msa0028440:CDS
TCAATGCATCGTTTGGTAAT+TGG 0.266872 1_1:+53356521 None:intergenic
CTCCTCCGCGCCTCGACGTT+CGG 0.271027 1_1:+53357138 None:intergenic
TGAGATCTTCTTCTCCTTCT+CGG 0.271871 1_1:-53357311 Msa0028440:CDS
TAACTTTATGTCAATATAAA+AGG 0.278881 1_1:-53355397 Msa0028440:three_prime_UTR
TGTGATGTAGAAGTATAAAT+TGG 0.285153 1_1:-53356693 Msa0028440:intron
AGTGTTATTGTTAGATTAAT+GGG 0.292224 1_1:-53357846 Msa0028440:CDS
CTCGTATTCAAGGATTTAAT+TGG 0.294796 1_1:-53355696 Msa0028440:CDS
TTTCTTCTTGAACTTATCTT+TGG 0.296526 1_1:+53357066 None:intergenic
GTAACATTGATAATGGATTA+TGG 0.301713 1_1:-53357608 Msa0028440:CDS
ATCCGAGGAGGAGGCAAAAT+TGG 0.302398 1_1:-53356114 Msa0028440:CDS
CCTTCCTGTTCTGGAACTTA+AGG 0.317482 1_1:-53358030 Msa0028440:CDS
AAGCACAATGATTCGATCTA+TGG 0.334299 1_1:-53357483 Msa0028440:CDS
GAATCATTGTGCTTTGGTTC+AGG 0.335322 1_1:+53357492 None:intergenic
ATCAGATTTGGTCAGAGTTT+CGG 0.339373 1_1:-53355863 Msa0028440:CDS
GACTCATTACCATCGGATTC+CGG 0.344614 1_1:-53357819 Msa0028440:CDS
TGCGTTTGCCAGAAAGAAAA+TGG 0.347612 1_1:+53358271 None:intergenic
ACCGTGGAAGGCGGTGTCTA+AGG 0.350723 1_1:-53355898 Msa0028440:CDS
AGATGAGTGGTTGGGGTGAA+TGG 0.352171 1_1:-53355758 Msa0028440:CDS
CTCTGTGTCGGTAAGTGAAT+TGG 0.356274 1_1:+53356918 None:intergenic
GATGATTATGATTATTCATC+TGG 0.360249 1_1:+53357688 None:intergenic
CACACTCAAGCATATCTTAG+AGG 0.366231 1_1:-53357403 Msa0028440:CDS
CTTATCTTTGGCGAATTAAC+CGG 0.366934 1_1:+53357078 None:intergenic
TCTGAATCAGAGGATTTGTT+TGG 0.368561 1_1:-53355819 Msa0028440:CDS
GCATTTGTTCGGATCTCTCT+AGG 0.372895 1_1:+53357915 None:intergenic
AAGATCTCAGTGGTTTCATT+AGG 0.374295 1_1:+53357325 None:intergenic
CATTAGCAGTTGCATTTGTT+CGG 0.378994 1_1:+53357904 None:intergenic
TCCTCCGCGCCTCGACGTTC+GGG 0.379144 1_1:+53357139 None:intergenic
AGGAAAATGTCACAATCTTC+AGG 0.380114 1_1:+53356038 None:intergenic
AGAGGTGTGGAAGGAGGAAG+AGG 0.380578 1_1:-53357579 Msa0028440:CDS
GACATGGGGTGTATCGTGTA+TGG 0.382034 1_1:+53358551 None:intergenic
TGAGCTCGATCATCAAATCT+TGG 0.387356 1_1:+53357201 None:intergenic
AAAACAAGTCCTTCCTGTTC+TGG 0.392571 1_1:-53358039 Msa0028440:CDS
CTCCTCCGCATTCTTCTGTT+CGG 0.411229 1_1:-53357254 Msa0028440:CDS
GGGTGAATGGACGGTGGAGA+TGG 0.415119 1_1:-53355745 Msa0028440:CDS
ATTGTAATCAATGCATCGTT+TGG 0.416134 1_1:+53356514 None:intergenic
TCATTAGGACAATGGGAGAT+TGG 0.416704 1_1:+53357340 None:intergenic
TTCAAAACACAAGCTTTCTT+CGG 0.418538 1_1:+53355616 None:intergenic
GAGAGGAGACACATCAGATT+TGG 0.420115 1_1:-53355875 Msa0028440:CDS
AAACAGATGGGGTGCATGGC+TGG 0.422548 1_1:-53358318 Msa0028440:CDS
TTTAGGAATAGTAGTTGTAA+TGG 0.423730 1_1:-53358492 Msa0028440:five_prime_UTR
CGACGTTCGGGACATTCCTC+CGG 0.426000 1_1:+53357151 None:intergenic
TTCTCTGATTTCTCCGGAGA+AGG 0.428779 1_1:+53358122 None:intergenic
TTCGAGCGGTTTAATAGAAC+CGG 0.430201 1_1:-53357288 Msa0028440:CDS
TCTAGGGTGAAGCCCTCCTT+TGG 0.433294 1_1:+53357932 None:intergenic
ATTGTTGCTTCTCAAGTAGA+AGG 0.435159 1_1:+53356018 None:intergenic
TCATCTTTCTCTGATTTCTC+CGG 0.442174 1_1:+53358116 None:intergenic
CGTTGCAGGAAATTCTTAAC+CGG 0.442389 1_1:-53355935 Msa0028440:CDS
GGCAGAGGAGATGAGTGGTT+GGG 0.443076 1_1:-53355766 Msa0028440:CDS
TTTATATTGACATAAAGTTA+TGG 0.445590 1_1:+53355400 None:intergenic
GGAGATGGGAGATGTCGTTT+TGG 0.449346 1_1:-53355730 Msa0028440:CDS
AGGAAGGTACAAGGTCTTCA+TGG 0.452464 1_1:-53358010 Msa0028440:CDS
TAGATCGAATCATTGTGCTT+TGG 0.455948 1_1:+53357486 None:intergenic
GAGAAAAGTGATTGATCCTA+AGG 0.458864 1_1:-53357004 Msa0028440:CDS
AGTGGTTTCATTAGGACAAT+GGG 0.462877 1_1:+53357333 None:intergenic
GTCAATATAAAAGGAAGTGT+TGG 0.463959 1_1:-53355388 Msa0028440:three_prime_UTR
CCTTCCTTAAGTTCCAGAAC+AGG 0.464066 1_1:+53358026 None:intergenic
GGAAGAGGGAAGCAGAATAA+GGG 0.466407 1_1:-53357564 Msa0028440:CDS
CGAACGTCGAGGCGCGGAGG+AGG 0.467855 1_1:-53357137 Msa0028440:CDS
GAGCTCGATCATCAAATCTT+GGG 0.471607 1_1:+53357202 None:intergenic
GACGTTCGGGACATTCCTCC+GGG 0.473521 1_1:+53357152 None:intergenic
AATTGGAGGAGTACAAAGAA+GGG 0.474862 1_1:-53356676 Msa0028440:CDS
ACATGGGGTGTATCGTGTAT+GGG 0.479244 1_1:+53358552 None:intergenic
GACGAGTCAAGAACCGATAC+CGG 0.481313 1_1:+53356575 None:intergenic
GGTGAATGGACGGTGGAGAT+GGG 0.481425 1_1:-53355744 Msa0028440:CDS
TGGTGTGTTGAAGAAGGATA+TGG 0.481990 1_1:-53355787 Msa0028440:CDS
CCGTGGAAGGCGGTGTCTAA+GGG 0.482299 1_1:-53355897 Msa0028440:CDS
CATTGTTAAATGTGGTGGTT+AGG 0.486475 1_1:-53355360 Msa0028440:three_prime_UTR
CAACGGAGCAATGTCGGCTC+GGG 0.488675 1_1:-53357711 Msa0028440:CDS
CCCTTAGACACCGCCTTCCA+CGG 0.493681 1_1:+53355897 None:intergenic
AAGTCGATCTCTCGAGACTC+TGG 0.494700 1_1:+53357767 None:intergenic
GTTCCGACGAGCACGAGGAC+TGG 0.496097 1_1:+53357230 None:intergenic
AGGAATAGTAGTTGTAATGG+AGG 0.501411 1_1:-53358489 Msa0028440:five_prime_UTR
GATATGTGGAGTGCAGGAGA+AGG 0.501417 1_1:-53356140 Msa0028440:CDS
CGAACAGAAGAATGCGGAGG+AGG 0.504857 1_1:+53357255 None:intergenic
AGAGAAAGATGATGGCAAAC+CGG 0.507452 1_1:-53358105 Msa0028440:CDS
AGAAGGAAAATCCGAGGAGG+AGG 0.508110 1_1:-53356123 Msa0028440:CDS
TGCCAGAAAGAAAATGGTTA+CGG 0.509581 1_1:+53358277 None:intergenic
TCAACGGAGCAATGTCGGCT+CGG 0.510393 1_1:-53357712 Msa0028440:CDS
CATCGAACGACTCGTATTCA+AGG 0.510869 1_1:-53355706 Msa0028440:CDS
GAAGATGATATGTGGAGTGC+AGG 0.512219 1_1:-53356146 Msa0028440:CDS
TGAGAAGCAACAATTTCGTA+AGG 0.515455 1_1:-53356009 Msa0028440:CDS
TCGGAGGATAAGAGAGAGGG+AGG 0.516634 1_1:-53355844 Msa0028440:CDS
CATTTGTTCGGATCTCTCTA+GGG 0.518783 1_1:+53357916 None:intergenic
GAGTGGTTGGGGTGAATGGA+CGG 0.519523 1_1:-53355754 Msa0028440:CDS
AATAGTAGTTGTAATGGAGG+AGG 0.520542 1_1:-53358486 Msa0028440:five_prime_UTR
AATTCTCAAGAGAGGCTCCT+AGG 0.521742 1_1:-53357986 Msa0028440:CDS
GATCCTAAGGTGAAAATGTT+GGG 0.522669 1_1:-53356991 Msa0028440:CDS
AAATTGGAGGAGTACAAAGA+AGG 0.523276 1_1:-53356677 Msa0028440:CDS
GCGGAGGAGGGTTAGTAATG+AGG 0.525806 1_1:-53357124 Msa0028440:CDS
CTTCTCCTTCTCGGTTCGAG+CGG 0.526908 1_1:-53357302 Msa0028440:CDS
GATTTGTTTGGAGTGATATG+TGG 0.528271 1_1:-53355807 Msa0028440:CDS
CAGTGGTTTCATTAGGACAA+TGG 0.528722 1_1:+53357332 None:intergenic
CGGAGGAGGAGAATCATAAC+CGG 0.529851 1_1:+53357269 None:intergenic
CATGGGGTGTATCGTGTATG+GGG 0.533503 1_1:+53358553 None:intergenic
ACGGTGGTAACCTTTGATTC+CGG 0.533627 1_1:+53355916 None:intergenic
TTGATCTCTTTAACCGGTGA+CGG 0.536844 1_1:+53358071 None:intergenic
TGGCAGAGGAGATGAGTGGT+TGG 0.536868 1_1:-53355767 Msa0028440:CDS
GAGAAATCAGAGAAAGATGA+TGG 0.540245 1_1:-53358113 Msa0028440:CDS
AGAAAAGGTTGAAGATGCGT+GGG 0.541387 1_1:-53357452 Msa0028440:CDS
AAAACATATTCACAAACAGA+TGG 0.541883 1_1:-53358331 Msa0028440:CDS
AAACATATTCACAAACAGAT+GGG 0.541977 1_1:-53358330 Msa0028440:CDS
GTTACAACCTAGTCCGGTAT+CGG 0.542307 1_1:-53356588 Msa0028440:CDS
AGAAGCAACAATTTCGTAAG+GGG 0.542314 1_1:-53356007 Msa0028440:CDS
GGCGTTGTCATCACCGTCAC+CGG 0.544136 1_1:-53358084 Msa0028440:CDS
GAGGTGTGGAAGGAGGAAGA+GGG 0.546629 1_1:-53357578 Msa0028440:CDS
TCAAGAACCGATACCGGACT+AGG 0.547333 1_1:+53356581 None:intergenic
GCAACTGCTCAGATAGCGAC+AGG 0.550497 1_1:-53357042 Msa0028440:CDS
AATGGTAGAGGTGTGGAAGG+AGG 0.550698 1_1:-53357585 Msa0028440:CDS
TAGCGACAGGAGGATCTCCT+AGG 0.552987 1_1:-53357029 Msa0028440:CDS
GCAGAGGAGATGAGTGGTTG+GGG 0.553665 1_1:-53355765 Msa0028440:CDS
TACACTCAACGGAGCAATGT+CGG 0.553892 1_1:-53357717 Msa0028440:CDS
GAGAAAAGGTTGAAGATGCG+TGG 0.554159 1_1:-53357453 Msa0028440:CDS
TGATTCTAGAAGAGTGTCTC+CGG 0.554213 1_1:-53357097 Msa0028440:CDS
TGTCGAGATCTTGAGAAGGT+TGG 0.554441 1_1:+53357424 None:intergenic
TCACAAACAGATGGGGTGCA+TGG 0.555175 1_1:-53358322 Msa0028440:CDS
AAACGAGTTACAACCTAGTC+CGG 0.557491 1_1:-53356594 Msa0028440:CDS
GATATGTGGTGTGTTGAAGA+AGG 0.557747 1_1:-53355793 Msa0028440:CDS
CGGAATCAGAAGATGATATG+TGG 0.558896 1_1:-53356154 Msa0028440:CDS
CAACACTAAAAGATTACCTC+CGG 0.560984 1_1:-53358241 Msa0028440:intron
GAACGTCGAGGCGCGGAGGA+GGG 0.561377 1_1:-53357136 Msa0028440:CDS
AAGCTTCGACGCTGCAGAGG+CGG 0.561968 1_1:-53355971 Msa0028440:CDS
CGAGGAGGAGGCAAAATTGG+AGG 0.562051 1_1:-53356111 Msa0028440:CDS
ATTGATAATGGATTATGGAA+TGG 0.563730 1_1:-53357603 Msa0028440:CDS
TCTATGGAGAGATTGAGAAA+AGG 0.563984 1_1:-53357467 Msa0028440:CDS
GCAGAATAAGGGAACAATGA+TGG 0.564406 1_1:-53357553 Msa0028440:CDS
TCAAAGGTTACCACCGTGGA+AGG 0.565345 1_1:-53355910 Msa0028440:CDS
TGGAATGGTAGAGGTGTGGA+AGG 0.568683 1_1:-53357588 Msa0028440:CDS
GAAGGCGGTGTCTAAGGGAG+AGG 0.569525 1_1:-53355892 Msa0028440:CDS
AAATTCTTAACCGGAATCAA+AGG 0.570524 1_1:-53355926 Msa0028440:CDS
GTTGAAGAAGGATATGGCAG+AGG 0.570762 1_1:-53355781 Msa0028440:CDS
GGTCCAGTCCTCGTGCTCGT+CGG 0.572382 1_1:-53357233 Msa0028440:CDS
CGGAGGAGGGTTAGTAATGA+GGG 0.573458 1_1:-53357123 Msa0028440:CDS
TGATCCTAAGGTGAAAATGT+TGG 0.573766 1_1:-53356992 Msa0028440:CDS
TGTGTCGGTAAGTGAATTGG+AGG 0.575594 1_1:+53356921 None:intergenic
GCAATGCAATGCGTAATATG+AGG 0.575903 1_1:-53355319 Msa0028440:three_prime_UTR
GATGTAGAAGTATAAATTGG+AGG 0.575910 1_1:-53356690 Msa0028440:intron
AAGAGTCTCATGAATGACAT+GGG 0.577717 1_1:+53358536 None:intergenic
GGGTCTTGACTCATTACCAT+CGG 0.577743 1_1:-53357826 Msa0028440:CDS
AGCTTTGCGCCGGAATCCGA+TGG 0.579218 1_1:+53357810 None:intergenic
GAGGGAGGAATCTGAATCAG+AGG 0.580288 1_1:-53355829 Msa0028440:CDS
TTAAACCGCTCGAACCGAGA+AGG 0.580662 1_1:+53357297 None:intergenic
TGGATGATCATTGTTAAATG+TGG 0.580980 1_1:-53355368 Msa0028440:three_prime_UTR
CAAAAGCTTCGACGCTGCAG+AGG 0.581028 1_1:-53355974 Msa0028440:CDS
GAGAAGCAACAATTTCGTAA+GGG 0.581516 1_1:-53356008 Msa0028440:CDS
TGGAGGAGTACAAAGAAGGG+AGG 0.584753 1_1:-53356673 Msa0028440:CDS
ATGATCATTGTTAAATGTGG+TGG 0.585349 1_1:-53355365 Msa0028440:three_prime_UTR
TGGGTGTAGCAGAGGATGAA+TGG 0.587182 1_1:-53356972 Msa0028440:CDS
GCAACAATTTCGTAAGGGGA+AGG 0.588925 1_1:-53356003 Msa0028440:CDS
CCTGTTCTGGAACTTAAGGA+AGG 0.590426 1_1:-53358026 Msa0028440:CDS
AGAGCAGTTGTTGATGCCAA+AGG 0.590682 1_1:-53357948 Msa0028440:CDS
CTCAAGTTCCGACGAGCACG+AGG 0.592627 1_1:+53357225 None:intergenic
AGATTTGGTCAGAGTTTCGG+AGG 0.592725 1_1:-53355860 Msa0028440:CDS
AAAGAGTCTCATGAATGACA+TGG 0.593680 1_1:+53358535 None:intergenic
GAGCCAAAATTGAAGTCCAA+CGG 0.595174 1_1:-53357741 Msa0028440:CDS
TGCATTGATTACAATTACAA+AGG 0.595599 1_1:-53356506 Msa0028440:intron
TAACACTGCAGATCAATCTA+CGG 0.598839 1_1:-53356174 Msa0028440:intron
GAAGCTATTCTCTGAAACTG+TGG 0.600223 1_1:+53356948 None:intergenic
GCAGTTGTTGATGCCAAAGG+AGG 0.602701 1_1:-53357945 Msa0028440:CDS
CTGCTATCTAAAGAAAGCCT+AGG 0.603053 1_1:+53357969 None:intergenic
AGATCTCTGAAGCTTTGCGC+CGG 0.603285 1_1:+53357800 None:intergenic
TGGTTGGGGTGAATGGACGG+TGG 0.603867 1_1:-53355751 Msa0028440:CDS
TGCAGGAGAAGGAAAATCCG+AGG 0.604176 1_1:-53356129 Msa0028440:CDS
CAGTTGTTGATGCCAAAGGA+GGG 0.608108 1_1:-53357944 Msa0028440:CDS
CATCGTTTGGTAATTGGTGA+AGG 0.612762 1_1:+53356527 None:intergenic
TTCGGGACATTCCTCCGGGT+CGG 0.614158 1_1:+53357156 None:intergenic
AGTCCAACGGTTACACTCAA+CGG 0.615578 1_1:-53357728 Msa0028440:CDS
TTCATGGAAATTCTCAAGAG+AGG 0.617223 1_1:-53357994 Msa0028440:CDS
TCCCGAACGTCGAGGCGCGG+AGG 0.618120 1_1:-53357140 Msa0028440:CDS
ATGTCCCGAACGTCGAGGCG+CGG 0.620714 1_1:-53357143 Msa0028440:CDS
CGAGAAACTCAAGTCCGACC+CGG 0.620759 1_1:-53357170 Msa0028440:CDS
TGGAACTTAAGGAAGGTACA+AGG 0.622837 1_1:-53358019 Msa0028440:CDS
GTTTCGGAGGATAAGAGAGA+GGG 0.632682 1_1:-53355847 Msa0028440:CDS
AATGGATTATGGAATGGTAG+AGG 0.636093 1_1:-53357597 Msa0028440:CDS
ATTATGGAATGGTAGAGGTG+TGG 0.636364 1_1:-53357592 Msa0028440:CDS
CCTTAAGTTCCAGAACAGGA+AGG 0.638162 1_1:+53358030 None:intergenic
GTCGGTAAGTGAATTGGAGG+TGG 0.638750 1_1:+53356924 None:intergenic
AGTTTCGGAGGATAAGAGAG+AGG 0.638762 1_1:-53355848 Msa0028440:CDS
GAAAATGTTGGGTGTAGCAG+AGG 0.641929 1_1:-53356980 Msa0028440:CDS
AAAGCTTCAGAGATCTGCGT+CGG 0.646577 1_1:-53357793 Msa0028440:CDS
GCTCCGTTGAGTGTAACCGT+TGG 0.647311 1_1:+53357725 None:intergenic
GATATGGCAGAGGAGATGAG+TGG 0.651978 1_1:-53355771 Msa0028440:CDS
AACATATTCACAAACAGATG+GGG 0.656999 1_1:-53358329 Msa0028440:CDS
TATTGTATATACCTCTGTGT+CGG 0.662309 1_1:+53356906 None:intergenic
GAAACTCAAGTCCGACCCGG+AGG 0.662948 1_1:-53357167 Msa0028440:CDS
ATGGGGTGTATCGTGTATGG+GGG 0.664475 1_1:+53358554 None:intergenic
AAGGTTACCACCGTGGAAGG+CGG 0.664796 1_1:-53355907 Msa0028440:CDS
ATCTCAATGTAACAAACTAG+AGG 0.676376 1_1:-53355643 Msa0028440:CDS
ACTGCTCAGATAGCGACAGG+AGG 0.695449 1_1:-53357039 Msa0028440:CDS
AGAGTCTCATGAATGACATG+GGG 0.699253 1_1:+53358537 None:intergenic
GAGGAATGTCCCGAACGTCG+AGG 0.702069 1_1:-53357148 Msa0028440:CDS
CTGGACCGAACAGAAGAATG+CGG 0.704211 1_1:+53357249 None:intergenic
GACCGAACAGAAGAATGCGG+AGG 0.708772 1_1:+53357252 None:intergenic
AGGAGAAGGAAAATCCGAGG+AGG 0.713917 1_1:-53356126 Msa0028440:CDS
AGTGTGTCGAGATCTTGAGA+AGG 0.714039 1_1:+53357420 None:intergenic
GAAGGAGAAGAAGATCTCAG+TGG 0.714102 1_1:+53357315 None:intergenic
GCAACTGCTAATGAGAACGA+CGG 0.720590 1_1:-53357894 Msa0028440:CDS
GGAATCAAAGGTTACCACCG+TGG 0.724541 1_1:-53355914 Msa0028440:CDS
CAGCATAGCGGAGATAACAG+CGG 0.731770 1_1:-53356618 Msa0028440:CDS
CAATTCACTTACCGACACAG+AGG 0.735333 1_1:-53356917 Msa0028440:intron
GGTGACGGTGATGACAACGC+CGG 0.742306 1_1:+53358086 None:intergenic
TAAGTTGCTGAACAGCATAG+CGG 0.749606 1_1:-53356630 Msa0028440:CDS
TTAGACACCGCCTTCCACGG+TGG 0.789038 1_1:+53355900 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATGTTAATTCTTA+TGG - chr1_1:53356247-53356266 Msa0028440:intron 10.0%
!! TCAGTAAAAAAAAAATTATT+CGG + chr1_1:53357149-53357168 None:intergenic 10.0%
!! TTTATATTGACATAAAGTTA+TGG + chr1_1:53358480-53358499 None:intergenic 15.0%
!! TAACTTTATGTCAATATAAA+AGG - chr1_1:53358480-53358499 Msa0028440:five_prime_UTR 15.0%
!!! AGTGTTATTGTTAGATTAAT+GGG - chr1_1:53356031-53356050 Msa0028440:CDS 20.0%
!!! TTAAATTCCTAATTTTGTCA+TGG + chr1_1:53358450-53358469 None:intergenic 20.0%
!!! TTATAGAGTGAGTTTGTTTT+AGG - chr1_1:53355368-53355387 Msa0028440:three_prime_UTR 25.0%
! TTTAGGAATAGTAGTTGTAA+TGG - chr1_1:53355385-53355404 Msa0028440:three_prime_UTR 25.0%
!! TAATTTTACTTTAGTGGTCA+TGG + chr1_1:53355485-53355504 None:intergenic 25.0%
!!! GCTGTTTAATTTTACTTTAG+TGG + chr1_1:53355491-53355510 None:intergenic 25.0%
! AAAACATATTCACAAACAGA+TGG - chr1_1:53355546-53355565 Msa0028440:three_prime_UTR 25.0%
! AAACATATTCACAAACAGAT+GGG - chr1_1:53355547-53355566 Msa0028440:three_prime_UTR 25.0%
! TGTAAAACCAAAACAAACAA+TGG + chr1_1:53355734-53355753 None:intergenic 25.0%
!! CAGTGTTATTGTTAGATTAA+TGG - chr1_1:53356030-53356049 Msa0028440:CDS 25.0%
! GATGATTATGATTATTCATC+TGG + chr1_1:53356192-53356211 None:intergenic 25.0%
! TCTTATGGTAACATTGATAA+TGG - chr1_1:53356262-53356281 Msa0028440:intron 25.0%
!!! GTAACATTGATAATGGATTA+TGG - chr1_1:53356269-53356288 Msa0028440:intron 25.0%
!!! ATTGATAATGGATTATGGAA+TGG - chr1_1:53356274-53356293 Msa0028440:intron 25.0%
! TTTCTTCTTGAACTTATCTT+TGG + chr1_1:53356814-53356833 None:intergenic 25.0%
!! TGTGATGTAGAAGTATAAAT+TGG - chr1_1:53357184-53357203 Msa0028440:CDS 25.0%
!!! TGCATTGATTACAATTACAA+AGG - chr1_1:53357371-53357390 Msa0028440:CDS 25.0%
! AAATAGGACAAGAAAAAAAG+AGG + chr1_1:53357437-53357456 None:intergenic 25.0%
!!! TTGTGATTTTGTTTATGTGT+CGG - chr1_1:53357790-53357809 Msa0028440:CDS 25.0%
! TTAATGACCATGACAAAATT+AGG - chr1_1:53358440-53358459 Msa0028440:five_prime_UTR 25.0%
!!! TTGCTTTTGTGTTGTTTTGA+TGG + chr1_1:53355422-53355441 None:intergenic 30.0%
AACATATTCACAAACAGATG+GGG - chr1_1:53355548-53355567 Msa0028440:three_prime_UTR 30.0%
GAAAGCAAAGAATGAATTAC+CGG + chr1_1:53355658-53355677 None:intergenic 30.0%
AACAAACAATGGAGATTAGT+CGG + chr1_1:53355723-53355742 None:intergenic 30.0%
! CTAATCTCCATTGTTTGTTT+TGG - chr1_1:53355724-53355743 Msa0028440:CDS 30.0%
!! TCACTTTTGATCTCTTTAAC+CGG + chr1_1:53355815-53355834 None:intergenic 30.0%
TATTGTATATACCTCTGTGT+CGG + chr1_1:53356974-53356993 None:intergenic 30.0%
! GATGTAGAAGTATAAATTGG+AGG - chr1_1:53357187-53357206 Msa0028440:CDS 30.0%
ATTGTAATCAATGCATCGTT+TGG + chr1_1:53357366-53357385 None:intergenic 30.0%
!! TTTTCTTGTCCTATTTCTCT+TGG - chr1_1:53357441-53357460 Msa0028440:CDS 30.0%
! TTGGATTTTCCAAGAGAAAT+AGG + chr1_1:53357453-53357472 None:intergenic 30.0%
! AAAAGTACACTACTACACAA+TGG + chr1_1:53357629-53357648 None:intergenic 30.0%
!!! TGTGTTTTAGTTGCTGATTA+TGG - chr1_1:53357671-53357690 Msa0028440:CDS 30.0%
AAATTCTTAACCGGAATCAA+AGG - chr1_1:53357951-53357970 Msa0028440:CDS 30.0%
CTCGTATTCAAGGATTTAAT+TGG - chr1_1:53358181-53358200 Msa0028440:intron 30.0%
ATCTCAATGTAACAAACTAG+AGG - chr1_1:53358234-53358253 Msa0028440:intron 30.0%
TTCAAAACACAAGCTTTCTT+CGG + chr1_1:53358264-53358283 None:intergenic 30.0%
TAACAGCATCAAAGAAAAAG+AGG - chr1_1:53358295-53358314 Msa0028440:CDS 30.0%
AACAGCATCAAAGAAAAAGA+GGG - chr1_1:53358296-53358315 Msa0028440:CDS 30.0%
! GTCAATATAAAAGGAAGTGT+TGG - chr1_1:53358489-53358508 Msa0028440:five_prime_UTR 30.0%
TGGATGATCATTGTTAAATG+TGG - chr1_1:53358509-53358528 Msa0028440:five_prime_UTR 30.0%
ATGATCATTGTTAAATGTGG+TGG - chr1_1:53358512-53358531 Msa0028440:five_prime_UTR 30.0%
AAGAGTCTCATGAATGACAT+GGG + chr1_1:53355344-53355363 None:intergenic 35.0%
AAAGAGTCTCATGAATGACA+TGG + chr1_1:53355345-53355364 None:intergenic 35.0%
AGGAATAGTAGTTGTAATGG+AGG - chr1_1:53355388-53355407 Msa0028440:three_prime_UTR 35.0%
! AATAGTAGTTGTAATGGAGG+AGG - chr1_1:53355391-53355410 Msa0028440:three_prime_UTR 35.0%
! TTTTACTTTAGTGGTCATGG+TGG + chr1_1:53355482-53355501 None:intergenic 35.0%
TGCCAGAAAGAAAATGGTTA+CGG + chr1_1:53355603-53355622 None:intergenic 35.0%
CAACACTAAAAGATTACCTC+CGG - chr1_1:53355636-53355655 Msa0028440:CDS 35.0%
TCATCTTTCTCTGATTTCTC+CGG + chr1_1:53355764-53355783 None:intergenic 35.0%
GAGAAATCAGAGAAAGATGA+TGG - chr1_1:53355764-53355783 Msa0028440:CDS 35.0%
TTCATGGAAATTCTCAAGAG+AGG - chr1_1:53355883-53355902 Msa0028440:CDS 35.0%
CATTAGCAGTTGCATTTGTT+CGG + chr1_1:53355976-53355995 None:intergenic 35.0%
! TAACCGTTGGACTTCAATTT+TGG + chr1_1:53356142-53356161 None:intergenic 35.0%
!! AATGGATTATGGAATGGTAG+AGG - chr1_1:53356280-53356299 Msa0028440:intron 35.0%
TAGATCGAATCATTGTGCTT+TGG + chr1_1:53356394-53356413 None:intergenic 35.0%
AAGCACAATGATTCGATCTA+TGG - chr1_1:53356394-53356413 Msa0028440:intron 35.0%
TCTATGGAGAGATTGAGAAA+AGG - chr1_1:53356410-53356429 Msa0028440:intron 35.0%
AGTGGTTTCATTAGGACAAT+GGG + chr1_1:53356547-53356566 None:intergenic 35.0%
AAGATCTCAGTGGTTTCATT+AGG + chr1_1:53356555-53356574 None:intergenic 35.0%
CTTATCTTTGGCGAATTAAC+CGG + chr1_1:53356802-53356821 None:intergenic 35.0%
!! TCAATCACTTTTCTCATCCT+AGG + chr1_1:53356868-53356887 None:intergenic 35.0%
GAGAAAAGTGATTGATCCTA+AGG - chr1_1:53356873-53356892 Msa0028440:intron 35.0%
TGATCCTAAGGTGAAAATGT+TGG - chr1_1:53356885-53356904 Msa0028440:intron 35.0%
GATCCTAAGGTGAAAATGTT+GGG - chr1_1:53356886-53356905 Msa0028440:intron 35.0%
! AAATTGGAGGAGTACAAAGA+AGG - chr1_1:53357200-53357219 Msa0028440:CDS 35.0%
! AATTGGAGGAGTACAAAGAA+GGG - chr1_1:53357201-53357220 Msa0028440:CDS 35.0%
!! TCTTGACTCGTCTTTTTACA+AGG - chr1_1:53357313-53357332 Msa0028440:CDS 35.0%
! TCAATGCATCGTTTGGTAAT+TGG + chr1_1:53357359-53357378 None:intergenic 35.0%
TGGAAAATCCAAAACCTATG+TGG - chr1_1:53357461-53357480 Msa0028440:CDS 35.0%
! TCAAAATGATTGGTCCACAT+AGG + chr1_1:53357478-53357497 None:intergenic 35.0%
!! GACCAATCATTTTGACATCA+GGG - chr1_1:53357483-53357502 Msa0028440:CDS 35.0%
AACCCTGATGTCAAAATGAT+TGG + chr1_1:53357488-53357507 None:intergenic 35.0%
TCTTTAGTTGTACAATCACG+TGG - chr1_1:53357567-53357586 Msa0028440:CDS 35.0%
CTTTAGTTGTACAATCACGT+GGG - chr1_1:53357568-53357587 Msa0028440:CDS 35.0%
TTTAGTTGTACAATCACGTG+GGG - chr1_1:53357569-53357588 Msa0028440:CDS 35.0%
AACTAAGTGACAGATTCGTT+GGG + chr1_1:53357602-53357621 None:intergenic 35.0%
TAACACTGCAGATCAATCTA+CGG - chr1_1:53357703-53357722 Msa0028440:CDS 35.0%
AGGAAAATGTCACAATCTTC+AGG + chr1_1:53357842-53357861 None:intergenic 35.0%
! ATTGTTGCTTCTCAAGTAGA+AGG + chr1_1:53357862-53357881 None:intergenic 35.0%
TGAGAAGCAACAATTTCGTA+AGG - chr1_1:53357868-53357887 Msa0028440:CDS 35.0%
GAGAAGCAACAATTTCGTAA+GGG - chr1_1:53357869-53357888 Msa0028440:CDS 35.0%
AGAAGCAACAATTTCGTAAG+GGG - chr1_1:53357870-53357889 Msa0028440:CDS 35.0%
! ATCAGATTTGGTCAGAGTTT+CGG - chr1_1:53358014-53358033 Msa0028440:CDS 35.0%
! TCTGAATCAGAGGATTTGTT+TGG - chr1_1:53358058-53358077 Msa0028440:CDS 35.0%
! GATTTGTTTGGAGTGATATG+TGG - chr1_1:53358070-53358089 Msa0028440:CDS 35.0%
AAAAAGAGGGAGGGAAAAAT+TGG - chr1_1:53358309-53358328 Msa0028440:CDS 35.0%
CATTGTTAAATGTGGTGGTT+AGG - chr1_1:53358517-53358536 Msa0028440:five_prime_UTR 35.0%
AGAGTCTCATGAATGACATG+GGG + chr1_1:53355343-53355362 None:intergenic 40.0%
! GACCGTAACCATTTTCTTTC+TGG - chr1_1:53355598-53355617 Msa0028440:exon 40.0%
TGCGTTTGCCAGAAAGAAAA+TGG + chr1_1:53355609-53355628 None:intergenic 40.0%
AGCAAAGAATGAATTACCGG+AGG + chr1_1:53355655-53355674 None:intergenic 40.0%
! ATGGAGATTAGTCGGTCATT+GGG + chr1_1:53355715-53355734 None:intergenic 40.0%
! AATGGAGATTAGTCGGTCAT+TGG + chr1_1:53355716-53355735 None:intergenic 40.0%
!!! TTTGGTTTTACAGCCTTCTC+CGG - chr1_1:53355742-53355761 Msa0028440:CDS 40.0%
! AGAGAAAGATGATGGCAAAC+CGG - chr1_1:53355772-53355791 Msa0028440:CDS 40.0%
TTGATCTCTTTAACCGGTGA+CGG + chr1_1:53355809-53355828 None:intergenic 40.0%
AAAACAAGTCCTTCCTGTTC+TGG - chr1_1:53355838-53355857 Msa0028440:CDS 40.0%
! TGGAACTTAAGGAAGGTACA+AGG - chr1_1:53355858-53355877 Msa0028440:CDS 40.0%
CTGCTATCTAAAGAAAGCCT+AGG + chr1_1:53355911-53355930 None:intergenic 40.0%
CATTTGTTCGGATCTCTCTA+GGG + chr1_1:53355964-53355983 None:intergenic 40.0%
GAGCCAAAATTGAAGTCCAA+CGG - chr1_1:53356136-53356155 Msa0028440:CDS 40.0%
ATTATGGAATGGTAGAGGTG+TGG - chr1_1:53356285-53356304 Msa0028440:intron 40.0%
GCAGAATAAGGGAACAATGA+TGG - chr1_1:53356324-53356343 Msa0028440:intron 40.0%
! GAATCATTGTGCTTTGGTTC+AGG + chr1_1:53356388-53356407 None:intergenic 40.0%
AGAAAAGGTTGAAGATGCGT+GGG - chr1_1:53356425-53356444 Msa0028440:intron 40.0%
CACACTCAAGCATATCTTAG+AGG - chr1_1:53356474-53356493 Msa0028440:intron 40.0%
TCATTAGGACAATGGGAGAT+TGG + chr1_1:53356540-53356559 None:intergenic 40.0%
CAGTGGTTTCATTAGGACAA+TGG + chr1_1:53356548-53356567 None:intergenic 40.0%
TGAGATCTTCTTCTCCTTCT+CGG - chr1_1:53356566-53356585 Msa0028440:CDS 40.0%
TTCGAGCGGTTTAATAGAAC+CGG - chr1_1:53356589-53356608 Msa0028440:CDS 40.0%
GAGCTCGATCATCAAATCTT+GGG + chr1_1:53356678-53356697 None:intergenic 40.0%
TGAGCTCGATCATCAAATCT+TGG + chr1_1:53356679-53356698 None:intergenic 40.0%
!! TGATTCTAGAAGAGTGTCTC+CGG - chr1_1:53356780-53356799 Msa0028440:intron 40.0%
! ACACCCAACATTTTCACCTT+AGG + chr1_1:53356892-53356911 None:intergenic 40.0%
!! GAAGCTATTCTCTGAAACTG+TGG + chr1_1:53356932-53356951 None:intergenic 40.0%
TAAGTTGCTGAACAGCATAG+CGG - chr1_1:53357247-53357266 Msa0028440:CDS 40.0%
AAACGAGTTACAACCTAGTC+CGG - chr1_1:53357283-53357302 Msa0028440:CDS 40.0%
! CATCGTTTGGTAATTGGTGA+AGG + chr1_1:53357353-53357372 None:intergenic 40.0%
!! TGATTGGTCCACATAGGTTT+TGG + chr1_1:53357472-53357491 None:intergenic 40.0%
!! GGACCAATCATTTTGACATC+AGG - chr1_1:53357482-53357501 Msa0028440:CDS 40.0%
TGACATCAGGGTTAATGACT+TGG - chr1_1:53357495-53357514 Msa0028440:CDS 40.0%
!! ATGACTTGGCATGACTTGTT+TGG - chr1_1:53357509-53357528 Msa0028440:CDS 40.0%
CAACTAAGTGACAGATTCGT+TGG + chr1_1:53357603-53357622 None:intergenic 40.0%
CGGAATCAGAAGATGATATG+TGG - chr1_1:53357723-53357742 Msa0028440:CDS 40.0%
! GTTAATTTTCGACACGTTGC+AGG - chr1_1:53357928-53357947 Msa0028440:CDS 40.0%
CGTTGCAGGAAATTCTTAAC+CGG - chr1_1:53357942-53357961 Msa0028440:CDS 40.0%
GATATGTGGTGTGTTGAAGA+AGG - chr1_1:53358084-53358103 Msa0028440:CDS 40.0%
TGGTGTGTTGAAGAAGGATA+TGG - chr1_1:53358090-53358109 Msa0028440:CDS 40.0%
AGCATCAAAGAAAAAGAGGG+AGG - chr1_1:53358299-53358318 Msa0028440:CDS 40.0%
GCATCAAAGAAAAAGAGGGA+GGG - chr1_1:53358300-53358319 Msa0028440:CDS 40.0%
ACATGGGGTGTATCGTGTAT+GGG + chr1_1:53355328-53355347 None:intergenic 45.0%
! AGATTAGTCGGTCATTGGGT+CGG + chr1_1:53355711-53355730 None:intergenic 45.0%
TTCTCTGATTTCTCCGGAGA+AGG + chr1_1:53355758-53355777 None:intergenic 45.0%
CCTTAAGTTCCAGAACAGGA+AGG + chr1_1:53355850-53355869 None:intergenic 45.0%
!! CCTTCCTGTTCTGGAACTTA+AGG - chr1_1:53355847-53355866 Msa0028440:CDS 45.0%
CCTTCCTTAAGTTCCAGAAC+AGG + chr1_1:53355854-53355873 None:intergenic 45.0%
!! CCTGTTCTGGAACTTAAGGA+AGG - chr1_1:53355851-53355870 Msa0028440:CDS 45.0%
! AGGAAGGTACAAGGTCTTCA+TGG - chr1_1:53355867-53355886 Msa0028440:CDS 45.0%
AATTCTCAAGAGAGGCTCCT+AGG - chr1_1:53355891-53355910 Msa0028440:CDS 45.0%
AGAGCAGTTGTTGATGCCAA+AGG - chr1_1:53355929-53355948 Msa0028440:CDS 45.0%
CAGTTGTTGATGCCAAAGGA+GGG - chr1_1:53355933-53355952 Msa0028440:CDS 45.0%
GCATTTGTTCGGATCTCTCT+AGG + chr1_1:53355965-53355984 None:intergenic 45.0%
GCAACTGCTAATGAGAACGA+CGG - chr1_1:53355983-53356002 Msa0028440:CDS 45.0%
GGGTCTTGACTCATTACCAT+CGG - chr1_1:53356051-53356070 Msa0028440:CDS 45.0%
GACTCATTACCATCGGATTC+CGG - chr1_1:53356058-53356077 Msa0028440:CDS 45.0%
AAAGCTTCAGAGATCTGCGT+CGG - chr1_1:53356084-53356103 Msa0028440:CDS 45.0%
AGTCCAACGGTTACACTCAA+CGG - chr1_1:53356149-53356168 Msa0028440:CDS 45.0%
TACACTCAACGGAGCAATGT+CGG - chr1_1:53356160-53356179 Msa0028440:CDS 45.0%
AGGAAGAGGGAAGCAGAATA+AGG - chr1_1:53356312-53356331 Msa0028440:intron 45.0%
GGAAGAGGGAAGCAGAATAA+GGG - chr1_1:53356313-53356332 Msa0028440:intron 45.0%
GAGAAAAGGTTGAAGATGCG+TGG - chr1_1:53356424-53356443 Msa0028440:intron 45.0%
TGTCGAGATCTTGAGAAGGT+TGG + chr1_1:53356456-53356475 None:intergenic 45.0%
AGTGTGTCGAGATCTTGAGA+AGG + chr1_1:53356460-53356479 None:intergenic 45.0%
TTAGAGGCTCTGCAGCTTAA+AGG - chr1_1:53356490-53356509 Msa0028440:intron 45.0%
GAAGGAGAAGAAGATCTCAG+TGG + chr1_1:53356565-53356584 None:intergenic 45.0%
GAAAATGTTGGGTGTAGCAG+AGG - chr1_1:53356897-53356916 Msa0028440:intron 45.0%
! TGTGTCGGTAAGTGAATTGG+AGG + chr1_1:53356959-53356978 None:intergenic 45.0%
! CTCTGTGTCGGTAAGTGAAT+TGG + chr1_1:53356962-53356981 None:intergenic 45.0%
CAATTCACTTACCGACACAG+AGG - chr1_1:53356960-53356979 Msa0028440:CDS 45.0%
AGAATCATAGCGAGTCATCG+TGG - chr1_1:53357042-53357061 Msa0028440:CDS 45.0%
GTTACAACCTAGTCCGGTAT+CGG - chr1_1:53357289-53357308 Msa0028440:CDS 45.0%
! CGTCTTTTTACAAGGACGAG+TGG - chr1_1:53357321-53357340 Msa0028440:CDS 45.0%
!!! AAGCAGACTCTGCTGTTTTG+AGG + chr1_1:53357548-53357567 None:intergenic 45.0%
GAAGATGATATGTGGAGTGC+AGG - chr1_1:53357731-53357750 Msa0028440:CDS 45.0%
GCAACAATTTCGTAAGGGGA+AGG - chr1_1:53357874-53357893 Msa0028440:CDS 45.0%
ACGGTGGTAACCTTTGATTC+CGG + chr1_1:53357964-53357983 None:intergenic 45.0%
GAGAGGAGACACATCAGATT+TGG - chr1_1:53358002-53358021 Msa0028440:CDS 45.0%
! AGATTTGGTCAGAGTTTCGG+AGG - chr1_1:53358017-53358036 Msa0028440:CDS 45.0%
AGTTTCGGAGGATAAGAGAG+AGG - chr1_1:53358029-53358048 Msa0028440:CDS 45.0%
GTTTCGGAGGATAAGAGAGA+GGG - chr1_1:53358030-53358049 Msa0028440:CDS 45.0%
GTTGAAGAAGGATATGGCAG+AGG - chr1_1:53358096-53358115 Msa0028440:CDS 45.0%
CATCGAACGACTCGTATTCA+AGG - chr1_1:53358171-53358190 Msa0028440:intron 45.0%
AAGAGGGAGGGAAAAATTGG+TGG - chr1_1:53358312-53358331 Msa0028440:CDS 45.0%
ATGGGGTGTATCGTGTATGG+GGG + chr1_1:53355326-53355345 None:intergenic 50.0%
CATGGGGTGTATCGTGTATG+GGG + chr1_1:53355327-53355346 None:intergenic 50.0%
GACATGGGGTGTATCGTGTA+TGG + chr1_1:53355329-53355348 None:intergenic 50.0%
TCACAAACAGATGGGGTGCA+TGG - chr1_1:53355555-53355574 Msa0028440:three_prime_UTR 50.0%
GCAGTTGTTGATGCCAAAGG+AGG - chr1_1:53355932-53355951 Msa0028440:CDS 50.0%
!! AGATCTCTGAAGCTTTGCGC+CGG + chr1_1:53356080-53356099 None:intergenic 50.0%
AAGTCGATCTCTCGAGACTC+TGG + chr1_1:53356113-53356132 None:intergenic 50.0%
! TGGAATGGTAGAGGTGTGGA+AGG - chr1_1:53356289-53356308 Msa0028440:intron 50.0%
! AATGGTAGAGGTGTGGAAGG+AGG - chr1_1:53356292-53356311 Msa0028440:intron 50.0%
TTAAACCGCTCGAACCGAGA+AGG + chr1_1:53356583-53356602 None:intergenic 50.0%
CGGAGGAGGAGAATCATAAC+CGG + chr1_1:53356611-53356630 None:intergenic 50.0%
CTCCTCCGCATTCTTCTGTT+CGG - chr1_1:53356623-53356642 Msa0028440:CDS 50.0%
CTGGACCGAACAGAAGAATG+CGG + chr1_1:53356631-53356650 None:intergenic 50.0%
! CGGAGGAGGGTTAGTAATGA+GGG - chr1_1:53356754-53356773 Msa0028440:intron 50.0%
TGGGTGTAGCAGAGGATGAA+TGG - chr1_1:53356905-53356924 Msa0028440:intron 50.0%
GTCGGTAAGTGAATTGGAGG+TGG + chr1_1:53356956-53356975 None:intergenic 50.0%
! TGGAGGAGTACAAAGAAGGG+AGG - chr1_1:53357204-53357223 Msa0028440:CDS 50.0%
! CAGCATAGCGGAGATAACAG+CGG - chr1_1:53357259-53357278 Msa0028440:CDS 50.0%
TCAAGAACCGATACCGGACT+AGG + chr1_1:53357299-53357318 None:intergenic 50.0%
GACGAGTCAAGAACCGATAC+CGG + chr1_1:53357305-53357324 None:intergenic 50.0%
GATATGTGGAGTGCAGGAGA+AGG - chr1_1:53357737-53357756 Msa0028440:CDS 50.0%
TGCAGGAGAAGGAAAATCCG+AGG - chr1_1:53357748-53357767 Msa0028440:CDS 50.0%
AGGAGAAGGAAAATCCGAGG+AGG - chr1_1:53357751-53357770 Msa0028440:CDS 50.0%
AGAAGGAAAATCCGAGGAGG+AGG - chr1_1:53357754-53357773 Msa0028440:CDS 50.0%
ATCCGAGGAGGAGGCAAAAT+TGG - chr1_1:53357763-53357782 Msa0028440:CDS 50.0%
! CTCCAATTTTGCCTCCTCCT+CGG + chr1_1:53357768-53357787 None:intergenic 50.0%
GGAATCAAAGGTTACCACCG+TGG - chr1_1:53357963-53357982 Msa0028440:CDS 50.0%
TCAAAGGTTACCACCGTGGA+AGG - chr1_1:53357967-53357986 Msa0028440:CDS 50.0%
GAGGGAGGAATCTGAATCAG+AGG - chr1_1:53358048-53358067 Msa0028440:CDS 50.0%
GATATGGCAGAGGAGATGAG+TGG - chr1_1:53358106-53358125 Msa0028440:CDS 50.0%
AGATGAGTGGTTGGGGTGAA+TGG - chr1_1:53358119-53358138 Msa0028440:CDS 50.0%
!! GGAGATGGGAGATGTCGTTT+TGG - chr1_1:53358147-53358166 Msa0028440:intron 50.0%
AAACAGATGGGGTGCATGGC+TGG - chr1_1:53355559-53355578 Msa0028440:three_prime_UTR 55.0%
! TCTAGGGTGAAGCCCTCCTT+TGG + chr1_1:53355948-53355967 None:intergenic 55.0%
GCTCCGTTGAGTGTAACCGT+TGG + chr1_1:53356155-53356174 None:intergenic 55.0%
! TCAACGGAGCAATGTCGGCT+CGG - chr1_1:53356165-53356184 Msa0028440:CDS 55.0%
! AGAGGTGTGGAAGGAGGAAG+AGG - chr1_1:53356298-53356317 Msa0028440:intron 55.0%
GAGGTGTGGAAGGAGGAAGA+GGG - chr1_1:53356299-53356318 Msa0028440:intron 55.0%
! CTTCTCCTTCTCGGTTCGAG+CGG - chr1_1:53356575-53356594 Msa0028440:CDS 55.0%
CGAACAGAAGAATGCGGAGG+AGG + chr1_1:53356625-53356644 None:intergenic 55.0%
GACCGAACAGAAGAATGCGG+AGG + chr1_1:53356628-53356647 None:intergenic 55.0%
CGAGAAACTCAAGTCCGACC+CGG - chr1_1:53356707-53356726 Msa0028440:intron 55.0%
! GCGGAGGAGGGTTAGTAATG+AGG - chr1_1:53356753-53356772 Msa0028440:intron 55.0%
GCAACTGCTCAGATAGCGAC+AGG - chr1_1:53356835-53356854 Msa0028440:intron 55.0%
ACTGCTCAGATAGCGACAGG+AGG - chr1_1:53356838-53356857 Msa0028440:intron 55.0%
TAGCGACAGGAGGATCTCCT+AGG - chr1_1:53356848-53356867 Msa0028440:intron 55.0%
CGAGGAGGAGGCAAAATTGG+AGG - chr1_1:53357766-53357785 Msa0028440:CDS 55.0%
CAAAAGCTTCGACGCTGCAG+AGG - chr1_1:53357903-53357922 Msa0028440:CDS 55.0%
AAGGTTACCACCGTGGAAGG+CGG - chr1_1:53357970-53357989 Msa0028440:CDS 55.0%
TCGGAGGATAAGAGAGAGGG+AGG - chr1_1:53358033-53358052 Msa0028440:CDS 55.0%
TGGCAGAGGAGATGAGTGGT+TGG - chr1_1:53358110-53358129 Msa0028440:CDS 55.0%
GGCAGAGGAGATGAGTGGTT+GGG - chr1_1:53358111-53358130 Msa0028440:CDS 55.0%
GCAGAGGAGATGAGTGGTTG+GGG - chr1_1:53358112-53358131 Msa0028440:CDS 55.0%
GAGTGGTTGGGGTGAATGGA+CGG - chr1_1:53358123-53358142 Msa0028440:CDS 55.0%
GGTGAATGGACGGTGGAGAT+GGG - chr1_1:53358133-53358152 Msa0028440:intron 55.0%
! GGTGACGGTGATGACAACGC+CGG + chr1_1:53355794-53355813 None:intergenic 60.0%
GGCGTTGTCATCACCGTCAC+CGG - chr1_1:53355793-53355812 Msa0028440:CDS 60.0%
AGCTTTGCGCCGGAATCCGA+TGG + chr1_1:53356070-53356089 None:intergenic 60.0%
!! CAACGGAGCAATGTCGGCTC+GGG - chr1_1:53356166-53356185 Msa0028440:CDS 60.0%
CTCAAGTTCCGACGAGCACG+AGG + chr1_1:53356655-53356674 None:intergenic 60.0%
GAAACTCAAGTCCGACCCGG+AGG - chr1_1:53356710-53356729 Msa0028440:intron 60.0%
TTCGGGACATTCCTCCGGGT+CGG + chr1_1:53356724-53356743 None:intergenic 60.0%
GACGTTCGGGACATTCCTCC+GGG + chr1_1:53356728-53356747 None:intergenic 60.0%
CGACGTTCGGGACATTCCTC+CGG + chr1_1:53356729-53356748 None:intergenic 60.0%
GAGGAATGTCCCGAACGTCG+AGG - chr1_1:53356729-53356748 Msa0028440:intron 60.0%
AAGCTTCGACGCTGCAGAGG+CGG - chr1_1:53357906-53357925 Msa0028440:CDS 60.0%
TTAGACACCGCCTTCCACGG+TGG + chr1_1:53357980-53357999 None:intergenic 60.0%
ACCGTGGAAGGCGGTGTCTA+AGG - chr1_1:53357979-53357998 Msa0028440:CDS 60.0%
CCCTTAGACACCGCCTTCCA+CGG + chr1_1:53357983-53358002 None:intergenic 60.0%
CCGTGGAAGGCGGTGTCTAA+GGG - chr1_1:53357980-53357999 Msa0028440:CDS 60.0%
GAAGGCGGTGTCTAAGGGAG+AGG - chr1_1:53357985-53358004 Msa0028440:CDS 60.0%
TGGTTGGGGTGAATGGACGG+TGG - chr1_1:53358126-53358145 Msa0028440:intron 60.0%
GGGTGAATGGACGGTGGAGA+TGG - chr1_1:53358132-53358151 Msa0028440:intron 60.0%
GGTCCAGTCCTCGTGCTCGT+CGG - chr1_1:53356644-53356663 Msa0028440:CDS 65.0%
GTTCCGACGAGCACGAGGAC+TGG + chr1_1:53356650-53356669 None:intergenic 65.0%
ATGTCCCGAACGTCGAGGCG+CGG - chr1_1:53356734-53356753 Msa0028440:intron 65.0%
TCCTCCGCGCCTCGACGTTC+GGG + chr1_1:53356741-53356760 None:intergenic 70.0%
CTCCTCCGCGCCTCGACGTT+CGG + chr1_1:53356742-53356761 None:intergenic 70.0%
GAACGTCGAGGCGCGGAGGA+GGG - chr1_1:53356741-53356760 Msa0028440:intron 70.0%
TCCCGAACGTCGAGGCGCGG+AGG - chr1_1:53356737-53356756 Msa0028440:intron 75.0%
CGAACGTCGAGGCGCGGAGG+AGG - chr1_1:53356740-53356759 Msa0028440:intron 75.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 53355319 53358580 53355319 ID=Msa0028440;Name=Msa0028440
chr1_1 mRNA 53355319 53358580 53355319 ID=Msa0028440-mRNA-1;Parent=Msa0028440;Name=Msa0028440-mRNA-1;_AED=0.07;_eAED=0.07;_QI=165|1|1|1|1|1|4|298|722
chr1_1 exon 53355319 53356185 53355319 ID=Msa0028440-mRNA-1:exon:15717;Parent=Msa0028440-mRNA-1
chr1_1 exon 53356507 53356705 53356507 ID=Msa0028440-mRNA-1:exon:15716;Parent=Msa0028440-mRNA-1
chr1_1 exon 53356918 53358144 53356918 ID=Msa0028440-mRNA-1:exon:15715;Parent=Msa0028440-mRNA-1
chr1_1 exon 53358242 53358580 53358242 ID=Msa0028440-mRNA-1:exon:15714;Parent=Msa0028440-mRNA-1
chr1_1 five_prime_UTR 53358416 53358580 53358416 ID=Msa0028440-mRNA-1:five_prime_utr;Parent=Msa0028440-mRNA-1
chr1_1 CDS 53358242 53358415 53358242 ID=Msa0028440-mRNA-1:cds;Parent=Msa0028440-mRNA-1
chr1_1 CDS 53356918 53358144 53356918 ID=Msa0028440-mRNA-1:cds;Parent=Msa0028440-mRNA-1
chr1_1 CDS 53356507 53356705 53356507 ID=Msa0028440-mRNA-1:cds;Parent=Msa0028440-mRNA-1
chr1_1 CDS 53355617 53356185 53355617 ID=Msa0028440-mRNA-1:cds;Parent=Msa0028440-mRNA-1
chr1_1 three_prime_UTR 53355319 53355616 53355319 ID=Msa0028440-mRNA-1:three_prime_utr;Parent=Msa0028440-mRNA-1
Gene Sequence

>Msa0028440

ATGACCACTAAAGTAAAATTAAACAGCAAAGAAGAAATGATAGACATTGATCACAACTTAGAAAAACATATTCACAAACAGATGGGGTGCATGGCTGGTTTGTTTCAAATCTTCGACCGTAACCATTTTCTTTCTGGCAAACGCATCTACAACAACACTAAAAGATTACCTCCGCCTTCTCCGGAGAAATCAGAGAAAGATGATGGCAAACCGGCGTTGTCATCACCGTCACCGGTTAAAGAGATCAAAAGTGAAACAAAACAAGTCCTTCCTGTTCTGGAACTTAAGGAAGGTACAAGGTCTTCATGGAAATTCTCAAGAGAGGCTCCTAGGCTTTCTTTAGATAGCAGAGCAGTTGTTGATGCCAAAGGAGGGCTTCACCCTAGAGAGATCCGAACAAATGCAACTGCTAATGAGAACGACGGTGATAAACAGCGTCGTTCTACTAGCAGTGTTATTGTTAGATTAATGGGTCTTGACTCATTACCATCGGATTCCGGCGCAAAGCTTCAGAGATCTGCGTCGGAGTCCAGAGTCTCGAGAGATCGACTTTCAGAGCCAAAATTGAAGTCCAACGGTTACACTCAACGGAGCAATGTCGGCTCGGGCCAGATGAATAATCATAATCATCATCGTTCTAATACTAATTCTAATGTTGTTAATGTTAATAATAATGTTAATTCTTATGGTAACATTGATAATGGATTATGGAATGGTAGAGGTGTGGAAGGAGGAAGAGGGAAGCAGAATAAGGGAACAATGATGGTGCAAAAGAAGAGTTTCTATGATTCTGCTGATTTCTTTCCTGAACCAAAGCACAATGATTCGATCTATGGAGAGATTGAGAAAAGGTTGAAGATGCGTGGGATTAACCAACCTTCTCAAGATCTCGACACACTCAAGCATATCTTAGAGGCTCTGCAGCTTAAAGGTTTGCTTCATTCGCAAAAACATGACCAATCTCCCATTGTCCTAATGAAACCACTGAGATCTTCTTCTCCTTCTCGGTTCGAGCGGTTTAATAGAACCGGTTATGATTCTCCTCCTCCGCATTCTTCTGTTCGGTCCAGTCCTCGTGCTCGTCGGAACTTGAGCCCAAGATTTGATGATCGAGCTCAAGTTAATTCGAGAAACTCAAGTCCGACCCGGAGGAATGTCCCGAACGTCGAGGCGCGGAGGAGGGTTAGTAATGAGGGTGTTGATTCTAGAAGAGTGTCTCCGGTTAATTCGCCAAAGATAAGTTCAAGAAGAAATGCAACTGCTCAGATAGCGACAGGAGGATCTCCTAGGATGAGAAAAGTGATTGATCCTAAGGTGAAAATGTTGGGTGTAGCAGAGGATGAATGGTCCACAGTTTCAGAGAATAGCTTCACCACCTCCAATTCACTTACCGACACAGAGAAGTATAAATTGGAGGAGTACAAAGAAGGGAGGAATCTGCTTGATAGATGTGATAAGTTGCTGAACAGCATAGCGGAGATAACAGCGGCAAACGAGTTACAACCTAGTCCGGTATCGGTTCTTGACTCGTCTTTTTACAAGGACGAGTGGTGCTCACCTTCACCAATTACCAAACGATGCATTGATTACAATTACAAAGATCAATCTACGGAATCAGAAGATGATATGTGGAGTGCAGGAGAAGGAAAATCCGAGGAGGAGGCAAAATTGGAGGATTGTGATTTTGTTTATGTGTCGGAGATTCTACGAGTTTCTAGTTACTTGCCTGAAGATTGTGACATTTTCCTTCTACTTGAGAAGCAACAATTTCGTAAGGGGAAGGATACTTCAAAAGCTTCGACGCTGCAGAGGCGGTTAATTTTCGACACGTTGCAGGAAATTCTTAACCGGAATCAAAGGTTACCACCGTGGAAGGCGGTGTCTAAGGGAGAGGAGACACATCAGATTTGGTCAGAGTTTCGGAGGATAAGAGAGAGGGAGGAATCTGAATCAGAGGATTTGTTTGGAGTGATATGTGGTGTGTTGAAGAAGGATATGGCAGAGGAGATGAGTGGTTGGGGTGAATGGACGGTGGAGATGGGAGATGTCGTTTTGGACATCGAACGACTCGTATTCAAGGATTTAATTGGCGAAACTATACAAGATCTTGCTTCATTTGCATCTCAATGTAACAAACTAGAGGCGCTCCGAAGAAAGCTTGTGTTTTGA
Protein sequence

>Msa0028440

MTTKVKLNSKEEMIDIDHNLEKHIHKQMGCMAGLFQIFDRNHFLSGKRIYNNTKRLPPPSPEKSEKDDGKPALSSPSPVKEIKSETKQVLPVLELKEGTRSSWKFSREAPRLSLDSRAVVDAKGGLHPREIRTNATANENDGDKQRRSTSSVIVRLMGLDSLPSDSGAKLQRSASESRVSRDRLSEPKLKSNGYTQRSNVGSGQMNNHNHHRSNTNSNVVNVNNNVNSYGNIDNGLWNGRGVEGGRGKQNKGTMMVQKKSFYDSADFFPEPKHNDSIYGEIEKRLKMRGINQPSQDLDTLKHILEALQLKGLLHSQKHDQSPIVLMKPLRSSSPSRFERFNRTGYDSPPPHSSVRSSPRARRNLSPRFDDRAQVNSRNSSPTRRNVPNVEARRRVSNEGVDSRRVSPVNSPKISSRRNATAQIATGGSPRMRKVIDPKVKMLGVAEDEWSTVSENSFTTSNSLTDTEKYKLEEYKEGRNLLDRCDKLLNSIAEITAANELQPSPVSVLDSSFYKDEWCSPSPITKRCIDYNYKDQSTESEDDMWSAGEGKSEEEAKLEDCDFVYVSEILRVSSYLPEDCDIFLLLEKQQFRKGKDTSKASTLQRRLIFDTLQEILNRNQRLPPWKAVSKGEETHQIWSEFRRIREREESESEDLFGVICGVLKKDMAEEMSGWGEWTVEMGDVVLDIERLVFKDLIGETIQDLASFASQCNKLEALRRKLVF*