AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0038320


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0038320 MtrunA17_Chr1g0201801 95.794 214 9 0 1 214 1 214 4.78e-149 412
Msa0038320 MtrunA17_Chr6g0472121 59.184 98 34 3 50 144 9 103 1.17e-23 98.6
Msa0038320 MtrunA17_Chr6g0464341 56.566 99 37 3 50 145 36 131 1.41e-23 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0038320 AT1G76770.1 55.118 127 54 1 1 124 1 127 7.35e-41 139
Msa0038320 AT1G76780.3 51.181 127 59 1 1 124 1 127 2.05e-35 132
Msa0038320 AT1G76780.2 51.181 127 59 1 1 124 1 127 2.05e-35 132

Find 36 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGTCCACCTTTGGTTCT+TGG 0.114619 1_1:+67742544 Msa0038320:CDS
AATCCCTAATGGAGTTGTTT+TGG 0.253932 1_1:+67742279 Msa0038320:CDS
CTAGGACGTCTCTTCTTAAC+AGG 0.266778 1_1:-67742508 None:intergenic
AAGAAAGAGCTGAAAATAAA+AGG 0.299279 1_1:+67742238 Msa0038320:CDS
TTATCCAAAACAACTCCATT+AGG 0.309228 1_1:-67742283 None:intergenic
GACAGGTCCTGCTCTGTCTT+TGG 0.343396 1_1:-67741710 None:intergenic
TATCCAAAACAACTCCATTA+GGG 0.362753 1_1:-67742282 None:intergenic
ATTGCTATGGAGGTTGAGTT+AGG 0.385137 1_1:+67741631 None:intergenic
ATGGCAACCTTGTCCACCTT+TGG 0.390972 1_1:+67742537 Msa0038320:CDS
AAGAAGTTTAAAATCCCTAA+TGG 0.416386 1_1:+67742268 Msa0038320:CDS
TGTCCACCTTTGGTTCTTGG+AGG 0.424444 1_1:+67742547 Msa0038320:CDS
GGCAAACTGAAAATCAGAAA+TGG 0.432210 1_1:-67741689 None:intergenic
CAGTTTGCCAAAGACAGAGC+AGG 0.497784 1_1:+67741703 Msa0038320:CDS
AGAAGAAGTGAAGGAAGAAG+AGG 0.509148 1_1:+67742399 Msa0038320:CDS
GTTTCTTTAGATAAGAAGAC+AGG 0.514312 1_1:-67741727 None:intergenic
TAGCAAAAGGAGTGCTGAGT+GGG 0.529464 1_1:+67742368 Msa0038320:CDS
GACATCAACATTAACAAAGA+TGG 0.531815 1_1:+67742157 Msa0038320:CDS
AAAGGAGTGCTGAGTGGGGT+GGG 0.541345 1_1:+67742373 Msa0038320:CDS
CTGAAAATCAGAAATGGAAG+TGG 0.544169 1_1:-67741683 None:intergenic
AAGTGCAAGAAAAGAAGTAA+AGG 0.549837 1_1:+67742453 Msa0038320:CDS
GAAGAAGTGAAGGAAGAAGA+GGG 0.550743 1_1:+67742400 Msa0038320:CDS
CCAAGAACCAAAGGTGGACA+AGG 0.550847 1_1:-67742544 None:intergenic
TACAGATGATAAGAGTGAAG+AGG 0.565214 1_1:+67742477 Msa0038320:CDS
CAAAGCTAAGTATTACGAAG+AGG 0.566739 1_1:+67742309 Msa0038320:CDS
ACCTTTGAGATGTGCTGTGA+GGG 0.582015 1_1:-67741764 None:intergenic
GCTAAGTATTACGAAGAGGA+AGG 0.582114 1_1:+67742313 Msa0038320:CDS
ACCCTCACAGCACATCTCAA+AGG 0.582279 1_1:+67741763 Msa0038320:CDS
AAAAGGAGTGCTGAGTGGGG+TGG 0.583010 1_1:+67742372 Msa0038320:CDS
AAAGGTGGACAAGGTTGCCA+TGG 0.584568 1_1:-67742535 None:intergenic
GGTGGGAATAGAAGAAGTGA+AGG 0.616063 1_1:+67742390 Msa0038320:CDS
GTTGATCCTCCAAGAACCAA+AGG 0.621557 1_1:-67742553 None:intergenic
GATCCTCCAAGAACCAAAGG+TGG 0.669806 1_1:-67742550 None:intergenic
AGCAAAAGGAGTGCTGAGTG+GGG 0.706883 1_1:+67742369 Msa0038320:CDS
AACCTTTGAGATGTGCTGTG+AGG 0.714535 1_1:-67741765 None:intergenic
AGAGACGTCCTAGAAAACCA+TGG 0.719572 1_1:+67742518 Msa0038320:CDS
ATAGCAAAAGGAGTGCTGAG+TGG 0.729591 1_1:+67742367 Msa0038320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTAATTTGTTTTCAC+AGG - chr1_1:67741969-67741988 None:intergenic 15.0%
!!! AAAATTTAATTTGTTTTCAC+AGG - chr1_1:67741969-67741988 None:intergenic 15.0%
! ATATGTTCATCATATCAACA+TGG - chr1_1:67742113-67742132 None:intergenic 25.0%
! AAGAAAGAGCTGAAAATAAA+AGG + chr1_1:67742238-67742257 Msa0038320:CDS 25.0%
!! AAGAAGTTTAAAATCCCTAA+TGG + chr1_1:67742268-67742287 Msa0038320:CDS 25.0%
! ATATGTTCATCATATCAACA+TGG - chr1_1:67742113-67742132 None:intergenic 25.0%
! AAGAAAGAGCTGAAAATAAA+AGG + chr1_1:67742238-67742257 Msa0038320:CDS 25.0%
!! AAGAAGTTTAAAATCCCTAA+TGG + chr1_1:67742268-67742287 Msa0038320:CDS 25.0%
GTTTCTTTAGATAAGAAGAC+AGG - chr1_1:67741730-67741749 None:intergenic 30.0%
CGTCTTATGATATGTCTATA+AGG + chr1_1:67741907-67741926 Msa0038320:intron 30.0%
CAACATGGTATCATTAACAT+AGG - chr1_1:67742098-67742117 None:intergenic 30.0%
TTGATATGATGAACATATGC+AGG + chr1_1:67742115-67742134 Msa0038320:intron 30.0%
GACATCAACATTAACAAAGA+TGG + chr1_1:67742157-67742176 Msa0038320:CDS 30.0%
! ATTTTCAGCTCTTTCTTGAA+TGG - chr1_1:67742235-67742254 None:intergenic 30.0%
TATCCAAAACAACTCCATTA+GGG - chr1_1:67742285-67742304 None:intergenic 30.0%
TTATCCAAAACAACTCCATT+AGG - chr1_1:67742286-67742305 None:intergenic 30.0%
AAGTGCAAGAAAAGAAGTAA+AGG + chr1_1:67742453-67742472 Msa0038320:CDS 30.0%
GTTTCTTTAGATAAGAAGAC+AGG - chr1_1:67741730-67741749 None:intergenic 30.0%
CGTCTTATGATATGTCTATA+AGG + chr1_1:67741907-67741926 Msa0038320:intron 30.0%
CAACATGGTATCATTAACAT+AGG - chr1_1:67742098-67742117 None:intergenic 30.0%
TTGATATGATGAACATATGC+AGG + chr1_1:67742115-67742134 Msa0038320:intron 30.0%
GACATCAACATTAACAAAGA+TGG + chr1_1:67742157-67742176 Msa0038320:CDS 30.0%
! ATTTTCAGCTCTTTCTTGAA+TGG - chr1_1:67742235-67742254 None:intergenic 30.0%
TATCCAAAACAACTCCATTA+GGG - chr1_1:67742285-67742304 None:intergenic 30.0%
TTATCCAAAACAACTCCATT+AGG - chr1_1:67742286-67742305 None:intergenic 30.0%
AAGTGCAAGAAAAGAAGTAA+AGG + chr1_1:67742453-67742472 Msa0038320:CDS 30.0%
CTGAAAATCAGAAATGGAAG+TGG - chr1_1:67741686-67741705 None:intergenic 35.0%
GGCAAACTGAAAATCAGAAA+TGG - chr1_1:67741692-67741711 None:intergenic 35.0%
! TTTTCACAGGCTATCATGAA+GGG - chr1_1:67741956-67741975 None:intergenic 35.0%
GGTACAAAAATTTCAGTGAG+TGG + chr1_1:67742178-67742197 Msa0038320:CDS 35.0%
! AATCCCTAATGGAGTTGTTT+TGG + chr1_1:67742279-67742298 Msa0038320:CDS 35.0%
CAAAGCTAAGTATTACGAAG+AGG + chr1_1:67742309-67742328 Msa0038320:CDS 35.0%
GAGAATAGTGATGCCAAAAA+CGG + chr1_1:67742342-67742361 Msa0038320:CDS 35.0%
!!! CCTTTTGCTATTTCCGTTTT+TGG - chr1_1:67742358-67742377 None:intergenic 35.0%
CCAAAAACGGAAATAGCAAA+AGG + chr1_1:67742355-67742374 Msa0038320:CDS 35.0%
! TCTTTTCTTGCACTTTGTTC+TGG - chr1_1:67742448-67742467 None:intergenic 35.0%
TACAGATGATAAGAGTGAAG+AGG + chr1_1:67742477-67742496 Msa0038320:CDS 35.0%
CTGAAAATCAGAAATGGAAG+TGG - chr1_1:67741686-67741705 None:intergenic 35.0%
GGCAAACTGAAAATCAGAAA+TGG - chr1_1:67741692-67741711 None:intergenic 35.0%
! TTTTCACAGGCTATCATGAA+GGG - chr1_1:67741956-67741975 None:intergenic 35.0%
GGTACAAAAATTTCAGTGAG+TGG + chr1_1:67742178-67742197 Msa0038320:CDS 35.0%
! AATCCCTAATGGAGTTGTTT+TGG + chr1_1:67742279-67742298 Msa0038320:CDS 35.0%
CAAAGCTAAGTATTACGAAG+AGG + chr1_1:67742309-67742328 Msa0038320:CDS 35.0%
GAGAATAGTGATGCCAAAAA+CGG + chr1_1:67742342-67742361 Msa0038320:CDS 35.0%
!!! CCTTTTGCTATTTCCGTTTT+TGG - chr1_1:67742358-67742377 None:intergenic 35.0%
CCAAAAACGGAAATAGCAAA+AGG + chr1_1:67742355-67742374 Msa0038320:CDS 35.0%
! TCTTTTCTTGCACTTTGTTC+TGG - chr1_1:67742448-67742467 None:intergenic 35.0%
TACAGATGATAAGAGTGAAG+AGG + chr1_1:67742477-67742496 Msa0038320:CDS 35.0%
! GTTTTCACAGGCTATCATGA+AGG - chr1_1:67741957-67741976 None:intergenic 40.0%
GCTAAGTATTACGAAGAGGA+AGG + chr1_1:67742313-67742332 Msa0038320:CDS 40.0%
AGAAGAAGTGAAGGAAGAAG+AGG + chr1_1:67742399-67742418 Msa0038320:CDS 40.0%
GAAGAAGTGAAGGAAGAAGA+GGG + chr1_1:67742400-67742419 Msa0038320:CDS 40.0%
! GTTTTCACAGGCTATCATGA+AGG - chr1_1:67741957-67741976 None:intergenic 40.0%
GCTAAGTATTACGAAGAGGA+AGG + chr1_1:67742313-67742332 Msa0038320:CDS 40.0%
AGAAGAAGTGAAGGAAGAAG+AGG + chr1_1:67742399-67742418 Msa0038320:CDS 40.0%
GAAGAAGTGAAGGAAGAAGA+GGG + chr1_1:67742400-67742419 Msa0038320:CDS 40.0%
!! ACCTTTGAGATGTGCTGTGA+GGG - chr1_1:67741767-67741786 None:intergenic 45.0%
!! AACCTTTGAGATGTGCTGTG+AGG - chr1_1:67741768-67741787 None:intergenic 45.0%
CCACTTGTAAGACTGTTCGA+CGG + chr1_1:67741872-67741891 Msa0038320:intron 45.0%
!! ATAGCAAAAGGAGTGCTGAG+TGG + chr1_1:67742367-67742386 Msa0038320:CDS 45.0%
!! TAGCAAAAGGAGTGCTGAGT+GGG + chr1_1:67742368-67742387 Msa0038320:CDS 45.0%
GGTGGGAATAGAAGAAGTGA+AGG + chr1_1:67742390-67742409 Msa0038320:CDS 45.0%
CTAGGACGTCTCTTCTTAAC+AGG - chr1_1:67742511-67742530 None:intergenic 45.0%
AGAGACGTCCTAGAAAACCA+TGG + chr1_1:67742518-67742537 Msa0038320:CDS 45.0%
! CAAGGTTGCCATGGTTTTCT+AGG - chr1_1:67742529-67742548 None:intergenic 45.0%
GTTGATCCTCCAAGAACCAA+AGG - chr1_1:67742556-67742575 None:intergenic 45.0%
!! ACCTTTGAGATGTGCTGTGA+GGG - chr1_1:67741767-67741786 None:intergenic 45.0%
!! AACCTTTGAGATGTGCTGTG+AGG - chr1_1:67741768-67741787 None:intergenic 45.0%
CCACTTGTAAGACTGTTCGA+CGG + chr1_1:67741872-67741891 Msa0038320:intron 45.0%
!! ATAGCAAAAGGAGTGCTGAG+TGG + chr1_1:67742367-67742386 Msa0038320:CDS 45.0%
!! TAGCAAAAGGAGTGCTGAGT+GGG + chr1_1:67742368-67742387 Msa0038320:CDS 45.0%
GGTGGGAATAGAAGAAGTGA+AGG + chr1_1:67742390-67742409 Msa0038320:CDS 45.0%
CTAGGACGTCTCTTCTTAAC+AGG - chr1_1:67742511-67742530 None:intergenic 45.0%
AGAGACGTCCTAGAAAACCA+TGG + chr1_1:67742518-67742537 Msa0038320:CDS 45.0%
! CAAGGTTGCCATGGTTTTCT+AGG - chr1_1:67742529-67742548 None:intergenic 45.0%
GTTGATCCTCCAAGAACCAA+AGG - chr1_1:67742556-67742575 None:intergenic 45.0%
CAGTTTGCCAAAGACAGAGC+AGG + chr1_1:67741703-67741722 Msa0038320:CDS 50.0%
ACCCTCACAGCACATCTCAA+AGG + chr1_1:67741763-67741782 Msa0038320:CDS 50.0%
CCGTCGAACAGTCTTACAAG+TGG - chr1_1:67741875-67741894 None:intergenic 50.0%
!! AGCAAAAGGAGTGCTGAGTG+GGG + chr1_1:67742369-67742388 Msa0038320:CDS 50.0%
AAAGGTGGACAAGGTTGCCA+TGG - chr1_1:67742538-67742557 None:intergenic 50.0%
ATGGCAACCTTGTCCACCTT+TGG + chr1_1:67742537-67742556 Msa0038320:CDS 50.0%
CCAAGAACCAAAGGTGGACA+AGG - chr1_1:67742547-67742566 None:intergenic 50.0%
!! CCTTGTCCACCTTTGGTTCT+TGG + chr1_1:67742544-67742563 Msa0038320:CDS 50.0%
!! TGTCCACCTTTGGTTCTTGG+AGG + chr1_1:67742547-67742566 Msa0038320:CDS 50.0%
GATCCTCCAAGAACCAAAGG+TGG - chr1_1:67742553-67742572 None:intergenic 50.0%
CAGTTTGCCAAAGACAGAGC+AGG + chr1_1:67741703-67741722 Msa0038320:CDS 50.0%
ACCCTCACAGCACATCTCAA+AGG + chr1_1:67741763-67741782 Msa0038320:CDS 50.0%
CCGTCGAACAGTCTTACAAG+TGG - chr1_1:67741875-67741894 None:intergenic 50.0%
!! AGCAAAAGGAGTGCTGAGTG+GGG + chr1_1:67742369-67742388 Msa0038320:CDS 50.0%
AAAGGTGGACAAGGTTGCCA+TGG - chr1_1:67742538-67742557 None:intergenic 50.0%
ATGGCAACCTTGTCCACCTT+TGG + chr1_1:67742537-67742556 Msa0038320:CDS 50.0%
CCAAGAACCAAAGGTGGACA+AGG - chr1_1:67742547-67742566 None:intergenic 50.0%
!! CCTTGTCCACCTTTGGTTCT+TGG + chr1_1:67742544-67742563 Msa0038320:CDS 50.0%
!! TGTCCACCTTTGGTTCTTGG+AGG + chr1_1:67742547-67742566 Msa0038320:CDS 50.0%
GATCCTCCAAGAACCAAAGG+TGG - chr1_1:67742553-67742572 None:intergenic 50.0%
GACAGGTCCTGCTCTGTCTT+TGG - chr1_1:67741713-67741732 None:intergenic 55.0%
!! AAAAGGAGTGCTGAGTGGGG+TGG + chr1_1:67742372-67742391 Msa0038320:CDS 55.0%
!! AAAGGAGTGCTGAGTGGGGT+GGG + chr1_1:67742373-67742392 Msa0038320:CDS 55.0%
GACAGGTCCTGCTCTGTCTT+TGG - chr1_1:67741713-67741732 None:intergenic 55.0%
!! AAAAGGAGTGCTGAGTGGGG+TGG + chr1_1:67742372-67742391 Msa0038320:CDS 55.0%
!! AAAGGAGTGCTGAGTGGGGT+GGG + chr1_1:67742373-67742392 Msa0038320:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 67741637 67742633 67741637 ID=Msa0038320;Name=Msa0038320
chr1_1 mRNA 67741637 67742633 67741637 ID=Msa0038320-mRNA-1;Parent=Msa0038320;Name=Msa0038320-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0|1|1|1|2|0|214
chr1_1 exon 67741637 67741784 67741637 ID=Msa0038320-mRNA-1:exon:22007;Parent=Msa0038320-mRNA-1
chr1_1 exon 67742137 67742633 67742137 ID=Msa0038320-mRNA-1:exon:22008;Parent=Msa0038320-mRNA-1
chr1_1 CDS 67741637 67741784 67741637 ID=Msa0038320-mRNA-1:cds;Parent=Msa0038320-mRNA-1
chr1_1 CDS 67742137 67742633 67742137 ID=Msa0038320-mRNA-1:cds;Parent=Msa0038320-mRNA-1
Gene Sequence

>Msa0038320

ATGGAGGTTGAGTTAGGTCTAAAGATTACAAGAACAAAAGATGACACCACTTCCATTTCTGATTTTCAGTTTGCCAAAGACAGAGCAGGACCTGTCTTCTTATCTAAAGAAACTGATGCAACTTTCACCCTCACAGCACATCTCAAAGGTTATAAGAAAGAGAACATTGACATCAACATTAACAAAGATGGTACAAAAATTTCAGTGAGTGGAGAAAAAGAAGTACAAGAAATGCTGATGATACCATTCAAGAAAGAGCTGAAAATAAAAGGATTTAGAAAGAAGTTTAAAATCCCTAATGGAGTTGTTTTGGATAAGATCAAAGCTAAGTATTACGAAGAGGAAGGAGTGCTGAGAATAGTGATGCCAAAAACGGAAATAGCAAAAGGAGTGCTGAGTGGGGTGGGAATAGAAGAAGTGAAGGAAGAAGAGGGTGCAAGTAGCATTATAGAAGAACCAGAACAAAGTGCAAGAAAAGAAGTAAAGGTTACAGATGATAAGAGTGAAGAGGAAAATGCTCCTGTTAAGAAGAGACGTCCTAGAAAACCATGGCAACCTTGTCCACCTTTGGTTCTTGGAGGATCAACTTTGCTTGTATCTATTATCTTTCTTGTAATGCACTATATTAGAGTGAGAAAGAGTTGA

Protein sequence

>Msa0038320

MEVELGLKITRTKDDTTSISDFQFAKDRAGPVFLSKETDATFTLTAHLKGYKKENIDININKDGTKISVSGEKEVQEMLMIPFKKELKIKGFRKKFKIPNGVVLDKIKAKYYEEEGVLRIVMPKTEIAKGVLSGVGIEEVKEEEGASSIIEEPEQSARKEVKVTDDKSEEENAPVKKRRPRKPWQPCPPLVLGGSTLLVSIIFLVMHYIRVRKS*