AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0044990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0044990 MtrunA17_Chr1g0211971 99.342 152 1 0 1 152 1 152 6.42e-109 306
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0044990 AT5G23290.1 72.368 152 39 2 1 152 1 149 2.91e-73 216

Find 66 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTCAAAGTGCTCTTTAT+TGG 0.161123 1_1:+76657128 Msa0044990:three_prime_UTR
AAACGAATTCTTTCAATAAA+AGG 0.178416 1_1:-76656874 None:intergenic
ATAAGTAAAATAATTTATTT+TGG 0.191605 1_1:+76655730 Msa0044990:five_prime_UTR
AATGACATTCGAGATTTAAA+AGG 0.255512 1_1:+76656964 Msa0044990:three_prime_UTR
TCGGAGCTGGAGGAGGGTTT+AGG 0.263067 1_1:+76655827 Msa0044990:five_prime_UTR
AGAACATTAGTTTGGACTAT+TGG 0.267818 1_1:+76656914 Msa0044990:three_prime_UTR
ATTCTATACCAGCTAACTTT+AGG 0.287756 1_1:-76656763 None:intergenic
CCTTTACTATATAATATTGT+AGG 0.292567 1_1:+76657151 Msa0044990:three_prime_UTR
GAGAAAATGGTGAGTAAAAT+TGG 0.295052 1_1:-76657097 None:intergenic
TCATTTCCTCATTTGATGTT+AGG 0.333820 1_1:+76657181 Msa0044990:three_prime_UTR
CATCACGTTCTCGTCGATAT+CGG 0.346200 1_1:+76656138 Msa0044990:CDS
CGGAGCTGGAGGAGGGTTTA+GGG 0.346867 1_1:+76655828 Msa0044990:five_prime_UTR
GATGCCTCAGAACATTAGTT+TGG 0.367543 1_1:+76656906 Msa0044990:three_prime_UTR
TGTTAGGCTCAGTCGAAGAT+AGG 0.389749 1_1:+76657197 Msa0044990:three_prime_UTR
AGTTAAGGAACAAGCAGATA+TGG 0.392343 1_1:+76655951 Msa0044990:CDS
GAGAGGGGCGATTAAATATC+AGG 0.394744 1_1:-76657232 None:intergenic
TCGCAGTAATCCTTTCCTTC+AGG 0.397139 1_1:-76656302 None:intergenic
GGAGGGATCTTGTGATTGTT+GGG 0.397497 1_1:+76657069 Msa0044990:three_prime_UTR
AGGAGGGATCTTGTGATTGT+TGG 0.405237 1_1:+76657068 Msa0044990:three_prime_UTR
TATTGTAATTGTAATCAAAT+CGG 0.414828 1_1:+76655808 Msa0044990:five_prime_UTR
TGAACAGTTGAAAGCAGTTA+AGG 0.432363 1_1:+76655936 Msa0044990:CDS
AGTGTGTAAAAGAGAGAAAA+TGG 0.438240 1_1:-76657110 None:intergenic
GATCTCTCTCTCCGTTCTCT+CGG 0.450391 1_1:+76656054 Msa0044990:CDS
CGGAAGCGGAGCGATGACTC+TGG 0.463988 1_1:+76655900 Msa0044990:CDS
CAAGACAGTCTCAACAATAT+CGG 0.466377 1_1:+76655988 Msa0044990:CDS
GCGATTAAATATCAGGAGTC+AGG 0.479053 1_1:-76657225 None:intergenic
TGTTGAGACTGTCTTGAAGA+AGG 0.480129 1_1:-76655981 None:intergenic
GTTAGGCTCAGTCGAAGATA+GGG 0.485542 1_1:+76657198 Msa0044990:three_prime_UTR
AATCAAATCGGAGCTGGAGG+AGG 0.507340 1_1:+76655820 Msa0044990:five_prime_UTR
ATCTTCATCCTAAAGTTAGC+TGG 0.507912 1_1:+76656755 Msa0044990:exon
GTTCTCGTCGATATCGGAAC+TGG 0.508671 1_1:+76656144 Msa0044990:CDS
TGAGGATCTTGAAGAGTAGC+AGG 0.512402 1_1:-76656117 None:intergenic
GAGATCGTTGAGAGCGGTGG+TGG 0.515505 1_1:-76656037 None:intergenic
CATAACACTGGTGTTAGAGT+TGG 0.520800 1_1:+76656830 Msa0044990:three_prime_UTR
ATGGTACAAATACAACGCCT+TGG 0.522364 1_1:-76656800 None:intergenic
GAAAACCATGCCTGAAGGAA+AGG 0.522733 1_1:+76656292 Msa0044990:intron
GCTTCGTCGAAGAGCGGAAG+CGG 0.528323 1_1:+76655886 Msa0044990:CDS
GAGGTACTAAAATAATAAAG+TGG 0.530105 1_1:+76655754 Msa0044990:five_prime_UTR
AATTGTAATCAAATCGGAGC+TGG 0.532751 1_1:+76655814 Msa0044990:five_prime_UTR
AAAACGTTGCGGATGAAGCT+GGG 0.534177 1_1:+76656700 Msa0044990:CDS
GTAATCCTTTCCTTCAGGCA+TGG 0.536343 1_1:-76656297 None:intergenic
CCTACAATATTATATAGTAA+AGG 0.541243 1_1:-76657151 None:intergenic
TAACTTCGATCAACTTGTTG+AGG 0.544631 1_1:+76656349 Msa0044990:CDS
GAGTTCAAGACGAGTGGTGG+CGG 0.544654 1_1:-76656013 None:intergenic
ACACAAGTCTTAATCAGAGC+AGG 0.545280 1_1:+76657019 Msa0044990:three_prime_UTR
CGAGATTTAAAAGGTTAATG+TGG 0.550201 1_1:+76656973 Msa0044990:three_prime_UTR
GGCGAGTTCAAGACGAGTGG+TGG 0.560196 1_1:-76656016 None:intergenic
GTTAATGTGGAAATAAGCAT+TGG 0.560930 1_1:+76656986 Msa0044990:three_prime_UTR
AGAGAGATCGTTGAGAGCGG+TGG 0.573255 1_1:-76656040 None:intergenic
ACTGAGCCTAACATCAAATG+AGG 0.577162 1_1:-76657187 None:intergenic
ATCAAATCGGAGCTGGAGGA+GGG 0.591866 1_1:+76655821 Msa0044990:five_prime_UTR
TGTAATCAAATCGGAGCTGG+AGG 0.598285 1_1:+76655817 Msa0044990:five_prime_UTR
GAGAAGCGGTGAGAGGAACA+AGG 0.602439 1_1:-76656086 None:intergenic
AAAATGGCTTCGTCGAAGAG+CGG 0.608939 1_1:+76655880 Msa0044990:exon
ATATCGACGAGAACGTGATG+AGG 0.609043 1_1:-76656135 None:intergenic
ACATAGAGAGAAGCGGTGAG+AGG 0.615765 1_1:-76656093 None:intergenic
AAAATGGTGAGTAAAATTGG+TGG 0.618170 1_1:-76657094 None:intergenic
AAACAGAAAACCATGCCTGA+AGG 0.621995 1_1:+76656287 Msa0044990:intron
ACCATAGAAAATCATAACAC+TGG 0.623201 1_1:+76656818 Msa0044990:three_prime_UTR
AGCAGGAACATAGAGAGAAG+CGG 0.640463 1_1:-76656100 None:intergenic
GCGGAGCGATGACTCTGGAG+AGG 0.643180 1_1:+76655905 Msa0044990:CDS
TAGTCCAAACTAATGTTCTG+AGG 0.643856 1_1:-76656910 None:intergenic
TTAGGCTCAGTCGAAGATAG+GGG 0.656337 1_1:+76657199 Msa0044990:three_prime_UTR
GAGAGAGAGATCGTTGAGAG+CGG 0.661545 1_1:-76656043 None:intergenic
CGGAACTGGTTATTTCGTCG+AGG 0.673821 1_1:+76656158 Msa0044990:CDS
GGTGGCGAGTTCAAGACGAG+TGG 0.675476 1_1:-76656019 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATAATTTATTTTGGTG+AGG + chr1_1:76655735-76655754 Msa0044990:five_prime_UTR 15.0%
!! TATTGTAATTGTAATCAAAT+CGG + chr1_1:76655808-76655827 Msa0044990:five_prime_UTR 15.0%
!! ACAGATCTAAATAAATTAAT+CGG - chr1_1:76656201-76656220 None:intergenic 15.0%
!!! TTAAACTTAATTGCAATTTT+CGG - chr1_1:76655711-76655730 None:intergenic 15.0%
!!! TAAAATAATTTATTTTGGTG+AGG + chr1_1:76655735-76655754 Msa0044990:five_prime_UTR 15.0%
!! TATTGTAATTGTAATCAAAT+CGG + chr1_1:76655808-76655827 Msa0044990:five_prime_UTR 15.0%
!! ACAGATCTAAATAAATTAAT+CGG - chr1_1:76656201-76656220 None:intergenic 15.0%
!! AGATCTAAATAAATTAATCG+GGG - chr1_1:76656199-76656218 None:intergenic 20.0%
!! CAGATCTAAATAAATTAATC+GGG - chr1_1:76656200-76656219 None:intergenic 20.0%
!! CTAGACAAAATATCAAATTA+GGG - chr1_1:76656571-76656590 None:intergenic 20.0%
!!! AATTCAATTACTTTTTTCCA+AGG + chr1_1:76656783-76656802 Msa0044990:three_prime_UTR 20.0%
!! AAACGAATTCTTTCAATAAA+AGG - chr1_1:76656877-76656896 None:intergenic 20.0%
!! CCTACAATATTATATAGTAA+AGG - chr1_1:76657154-76657173 None:intergenic 20.0%
!! CCTTTACTATATAATATTGT+AGG + chr1_1:76657151-76657170 Msa0044990:three_prime_UTR 20.0%
!!! TAAACTTAATTGCAATTTTC+GGG - chr1_1:76655710-76655729 None:intergenic 20.0%
!! AGATCTAAATAAATTAATCG+GGG - chr1_1:76656199-76656218 None:intergenic 20.0%
!! CAGATCTAAATAAATTAATC+GGG - chr1_1:76656200-76656219 None:intergenic 20.0%
!! CTAGACAAAATATCAAATTA+GGG - chr1_1:76656571-76656590 None:intergenic 20.0%
!!! AATTCAATTACTTTTTTCCA+AGG + chr1_1:76656783-76656802 Msa0044990:three_prime_UTR 20.0%
!! AAACGAATTCTTTCAATAAA+AGG - chr1_1:76656877-76656896 None:intergenic 20.0%
!! CCTACAATATTATATAGTAA+AGG - chr1_1:76657154-76657173 None:intergenic 20.0%
!! CCTTTACTATATAATATTGT+AGG + chr1_1:76657151-76657170 Msa0044990:three_prime_UTR 20.0%
!! GAGGTACTAAAATAATAAAG+TGG + chr1_1:76655754-76655773 Msa0044990:five_prime_UTR 25.0%
!! TATCGTATATGCTTCTTTTT+AGG + chr1_1:76656220-76656239 Msa0044990:intron 25.0%
!! ATCGTATATGCTTCTTTTTA+GGG + chr1_1:76656221-76656240 Msa0044990:intron 25.0%
!!! GTGTTAGTTTTATACTAATG+TGG + chr1_1:76656494-76656513 Msa0044990:intron 25.0%
! CCTAGACAAAATATCAAATT+AGG - chr1_1:76656572-76656591 None:intergenic 25.0%
!! CCTAATTTGATATTTTGTCT+AGG + chr1_1:76656569-76656588 Msa0044990:intron 25.0%
! AAAAATATCAGAGAGAAAAC+TGG - chr1_1:76656606-76656625 None:intergenic 25.0%
!! AGTTTTCTCTCTGATATTTT+TGG + chr1_1:76656605-76656624 Msa0044990:intron 25.0%
!! TTTTAAATCTCGAATGTCAT+TGG - chr1_1:76656965-76656984 None:intergenic 25.0%
! AATGACATTCGAGATTTAAA+AGG + chr1_1:76656964-76656983 Msa0044990:three_prime_UTR 25.0%
!! AAACTTAATTGCAATTTTCG+GGG - chr1_1:76655709-76655728 None:intergenic 25.0%
!! GAGGTACTAAAATAATAAAG+TGG + chr1_1:76655754-76655773 Msa0044990:five_prime_UTR 25.0%
!! TATCGTATATGCTTCTTTTT+AGG + chr1_1:76656220-76656239 Msa0044990:intron 25.0%
!! ATCGTATATGCTTCTTTTTA+GGG + chr1_1:76656221-76656240 Msa0044990:intron 25.0%
!!! GTGTTAGTTTTATACTAATG+TGG + chr1_1:76656494-76656513 Msa0044990:intron 25.0%
! CCTAGACAAAATATCAAATT+AGG - chr1_1:76656572-76656591 None:intergenic 25.0%
!! CCTAATTTGATATTTTGTCT+AGG + chr1_1:76656569-76656588 Msa0044990:intron 25.0%
! AAAAATATCAGAGAGAAAAC+TGG - chr1_1:76656606-76656625 None:intergenic 25.0%
!! AGTTTTCTCTCTGATATTTT+TGG + chr1_1:76656605-76656624 Msa0044990:intron 25.0%
!! TTTTAAATCTCGAATGTCAT+TGG - chr1_1:76656965-76656984 None:intergenic 25.0%
! AATGACATTCGAGATTTAAA+AGG + chr1_1:76656964-76656983 Msa0044990:three_prime_UTR 25.0%
AATCGAGAACCTTCAAAAAA+TGG + chr1_1:76655864-76655883 Msa0044990:exon 30.0%
!!! TATGCTTCTTTTTAGGGTTA+TGG + chr1_1:76656227-76656246 Msa0044990:intron 30.0%
CTTGATGTAATTACAAATGC+AGG + chr1_1:76656662-76656681 Msa0044990:intron 30.0%
ATTCTATACCAGCTAACTTT+AGG - chr1_1:76656766-76656785 None:intergenic 30.0%
ACCATAGAAAATCATAACAC+TGG + chr1_1:76656818-76656837 Msa0044990:three_prime_UTR 30.0%
! ACCAGTGTTATGATTTTCTA+TGG - chr1_1:76656822-76656841 None:intergenic 30.0%
! AGAACATTAGTTTGGACTAT+TGG + chr1_1:76656914-76656933 Msa0044990:three_prime_UTR 30.0%
CGAGATTTAAAAGGTTAATG+TGG + chr1_1:76656973-76656992 Msa0044990:three_prime_UTR 30.0%
GTTAATGTGGAAATAAGCAT+TGG + chr1_1:76656986-76657005 Msa0044990:three_prime_UTR 30.0%
AAAATGGTGAGTAAAATTGG+TGG - chr1_1:76657097-76657116 None:intergenic 30.0%
GAGAAAATGGTGAGTAAAAT+TGG - chr1_1:76657100-76657119 None:intergenic 30.0%
AGTGTGTAAAAGAGAGAAAA+TGG - chr1_1:76657113-76657132 None:intergenic 30.0%
TCATTTCCTCATTTGATGTT+AGG + chr1_1:76657181-76657200 Msa0044990:three_prime_UTR 30.0%
AATCGAGAACCTTCAAAAAA+TGG + chr1_1:76655864-76655883 Msa0044990:exon 30.0%
!!! TATGCTTCTTTTTAGGGTTA+TGG + chr1_1:76656227-76656246 Msa0044990:intron 30.0%
CTTGATGTAATTACAAATGC+AGG + chr1_1:76656662-76656681 Msa0044990:intron 30.0%
ATTCTATACCAGCTAACTTT+AGG - chr1_1:76656766-76656785 None:intergenic 30.0%
ACCATAGAAAATCATAACAC+TGG + chr1_1:76656818-76656837 Msa0044990:three_prime_UTR 30.0%
! ACCAGTGTTATGATTTTCTA+TGG - chr1_1:76656822-76656841 None:intergenic 30.0%
! AGAACATTAGTTTGGACTAT+TGG + chr1_1:76656914-76656933 Msa0044990:three_prime_UTR 30.0%
CGAGATTTAAAAGGTTAATG+TGG + chr1_1:76656973-76656992 Msa0044990:three_prime_UTR 30.0%
GTTAATGTGGAAATAAGCAT+TGG + chr1_1:76656986-76657005 Msa0044990:three_prime_UTR 30.0%
AAAATGGTGAGTAAAATTGG+TGG - chr1_1:76657097-76657116 None:intergenic 30.0%
GAGAAAATGGTGAGTAAAAT+TGG - chr1_1:76657100-76657119 None:intergenic 30.0%
AGTGTGTAAAAGAGAGAAAA+TGG - chr1_1:76657113-76657132 None:intergenic 30.0%
TCATTTCCTCATTTGATGTT+AGG + chr1_1:76657181-76657200 Msa0044990:three_prime_UTR 30.0%
AATTGTAATCAAATCGGAGC+TGG + chr1_1:76655814-76655833 Msa0044990:five_prime_UTR 35.0%
TGAACAGTTGAAAGCAGTTA+AGG + chr1_1:76655936-76655955 Msa0044990:CDS 35.0%
AGTTAAGGAACAAGCAGATA+TGG + chr1_1:76655951-76655970 Msa0044990:CDS 35.0%
CAAGACAGTCTCAACAATAT+CGG + chr1_1:76655988-76656007 Msa0044990:CDS 35.0%
!! TAACTTCGATCAACTTGTTG+AGG + chr1_1:76656349-76656368 Msa0044990:CDS 35.0%
CTAATGTGGTTATTCTGTTG+TGG + chr1_1:76656508-76656527 Msa0044990:intron 35.0%
ATCTTCATCCTAAAGTTAGC+TGG + chr1_1:76656755-76656774 Msa0044990:exon 35.0%
TAGTCCAAACTAATGTTCTG+AGG - chr1_1:76656913-76656932 None:intergenic 35.0%
!! AGCACTCCATGCTTTTTATT+AGG + chr1_1:76657048-76657067 Msa0044990:three_prime_UTR 35.0%
!! ACTCCATGCTTTTTATTAGG+AGG + chr1_1:76657051-76657070 Msa0044990:three_prime_UTR 35.0%
!! CTCCATGCTTTTTATTAGGA+GGG + chr1_1:76657052-76657071 Msa0044990:three_prime_UTR 35.0%
ATCCCTCCTAATAAAAAGCA+TGG - chr1_1:76657057-76657076 None:intergenic 35.0%
! ACACTCAAAGTGCTCTTTAT+TGG + chr1_1:76657128-76657147 Msa0044990:three_prime_UTR 35.0%
AATTGTAATCAAATCGGAGC+TGG + chr1_1:76655814-76655833 Msa0044990:five_prime_UTR 35.0%
TGAACAGTTGAAAGCAGTTA+AGG + chr1_1:76655936-76655955 Msa0044990:CDS 35.0%
AGTTAAGGAACAAGCAGATA+TGG + chr1_1:76655951-76655970 Msa0044990:CDS 35.0%
CAAGACAGTCTCAACAATAT+CGG + chr1_1:76655988-76656007 Msa0044990:CDS 35.0%
!! TAACTTCGATCAACTTGTTG+AGG + chr1_1:76656349-76656368 Msa0044990:CDS 35.0%
CTAATGTGGTTATTCTGTTG+TGG + chr1_1:76656508-76656527 Msa0044990:intron 35.0%
ATCTTCATCCTAAAGTTAGC+TGG + chr1_1:76656755-76656774 Msa0044990:exon 35.0%
TAGTCCAAACTAATGTTCTG+AGG - chr1_1:76656913-76656932 None:intergenic 35.0%
!! AGCACTCCATGCTTTTTATT+AGG + chr1_1:76657048-76657067 Msa0044990:three_prime_UTR 35.0%
!! ACTCCATGCTTTTTATTAGG+AGG + chr1_1:76657051-76657070 Msa0044990:three_prime_UTR 35.0%
!! CTCCATGCTTTTTATTAGGA+GGG + chr1_1:76657052-76657071 Msa0044990:three_prime_UTR 35.0%
ATCCCTCCTAATAAAAAGCA+TGG - chr1_1:76657057-76657076 None:intergenic 35.0%
! ACACTCAAAGTGCTCTTTAT+TGG + chr1_1:76657128-76657147 Msa0044990:three_prime_UTR 35.0%
!!! TCGACGAAGCCATTTTTTGA+AGG - chr1_1:76655876-76655895 None:intergenic 40.0%
TGTTGAGACTGTCTTGAAGA+AGG - chr1_1:76655984-76656003 None:intergenic 40.0%
AAACAGAAAACCATGCCTGA+AGG + chr1_1:76656287-76656306 Msa0044990:intron 40.0%
GTCCAAGAAGAAAAACGTTG+CGG + chr1_1:76656689-76656708 Msa0044990:CDS 40.0%
! ATCCGCAACGTTTTTCTTCT+TGG - chr1_1:76656694-76656713 None:intergenic 40.0%
!!! TGAAGCTGGGCTTATTTTAC+AGG + chr1_1:76656713-76656732 Msa0044990:CDS 40.0%
ATGGTACAAATACAACGCCT+TGG - chr1_1:76656803-76656822 None:intergenic 40.0%
! CATAACACTGGTGTTAGAGT+TGG + chr1_1:76656830-76656849 Msa0044990:three_prime_UTR 40.0%
GATGCCTCAGAACATTAGTT+TGG + chr1_1:76656906-76656925 Msa0044990:three_prime_UTR 40.0%
ACACAAGTCTTAATCAGAGC+AGG + chr1_1:76657019-76657038 Msa0044990:three_prime_UTR 40.0%
ACTGAGCCTAACATCAAATG+AGG - chr1_1:76657190-76657209 None:intergenic 40.0%
GCGATTAAATATCAGGAGTC+AGG - chr1_1:76657228-76657247 None:intergenic 40.0%
!!! TCGACGAAGCCATTTTTTGA+AGG - chr1_1:76655876-76655895 None:intergenic 40.0%
TGTTGAGACTGTCTTGAAGA+AGG - chr1_1:76655984-76656003 None:intergenic 40.0%
AAACAGAAAACCATGCCTGA+AGG + chr1_1:76656287-76656306 Msa0044990:intron 40.0%
GTCCAAGAAGAAAAACGTTG+CGG + chr1_1:76656689-76656708 Msa0044990:CDS 40.0%
! ATCCGCAACGTTTTTCTTCT+TGG - chr1_1:76656694-76656713 None:intergenic 40.0%
!!! TGAAGCTGGGCTTATTTTAC+AGG + chr1_1:76656713-76656732 Msa0044990:CDS 40.0%
ATGGTACAAATACAACGCCT+TGG - chr1_1:76656803-76656822 None:intergenic 40.0%
! CATAACACTGGTGTTAGAGT+TGG + chr1_1:76656830-76656849 Msa0044990:three_prime_UTR 40.0%
GATGCCTCAGAACATTAGTT+TGG + chr1_1:76656906-76656925 Msa0044990:three_prime_UTR 40.0%
ACACAAGTCTTAATCAGAGC+AGG + chr1_1:76657019-76657038 Msa0044990:three_prime_UTR 40.0%
ACTGAGCCTAACATCAAATG+AGG - chr1_1:76657190-76657209 None:intergenic 40.0%
GCGATTAAATATCAGGAGTC+AGG - chr1_1:76657228-76657247 None:intergenic 40.0%
TGTAATCAAATCGGAGCTGG+AGG + chr1_1:76655817-76655836 Msa0044990:five_prime_UTR 45.0%
AAAATGGCTTCGTCGAAGAG+CGG + chr1_1:76655880-76655899 Msa0044990:exon 45.0%
! GGATTTTACTGCCGAGAGAA+CGG - chr1_1:76656068-76656087 None:intergenic 45.0%
AGCAGGAACATAGAGAGAAG+CGG - chr1_1:76656103-76656122 None:intergenic 45.0%
TGAGGATCTTGAAGAGTAGC+AGG - chr1_1:76656120-76656139 None:intergenic 45.0%
ATATCGACGAGAACGTGATG+AGG - chr1_1:76656138-76656157 None:intergenic 45.0%
CATCACGTTCTCGTCGATAT+CGG + chr1_1:76656138-76656157 Msa0044990:CDS 45.0%
GAAAACCATGCCTGAAGGAA+AGG + chr1_1:76656292-76656311 Msa0044990:intron 45.0%
GTAATCCTTTCCTTCAGGCA+TGG - chr1_1:76656300-76656319 None:intergenic 45.0%
TCGCAGTAATCCTTTCCTTC+AGG - chr1_1:76656305-76656324 None:intergenic 45.0%
AAAAACGTTGCGGATGAAGC+TGG + chr1_1:76656699-76656718 Msa0044990:CDS 45.0%
AAAACGTTGCGGATGAAGCT+GGG + chr1_1:76656700-76656719 Msa0044990:CDS 45.0%
AGGAGGGATCTTGTGATTGT+TGG + chr1_1:76657068-76657087 Msa0044990:three_prime_UTR 45.0%
GGAGGGATCTTGTGATTGTT+GGG + chr1_1:76657069-76657088 Msa0044990:three_prime_UTR 45.0%
TGTTAGGCTCAGTCGAAGAT+AGG + chr1_1:76657197-76657216 Msa0044990:three_prime_UTR 45.0%
GTTAGGCTCAGTCGAAGATA+GGG + chr1_1:76657198-76657217 Msa0044990:three_prime_UTR 45.0%
TTAGGCTCAGTCGAAGATAG+GGG + chr1_1:76657199-76657218 Msa0044990:three_prime_UTR 45.0%
TGTAATCAAATCGGAGCTGG+AGG + chr1_1:76655817-76655836 Msa0044990:five_prime_UTR 45.0%
AAAATGGCTTCGTCGAAGAG+CGG + chr1_1:76655880-76655899 Msa0044990:exon 45.0%
! GGATTTTACTGCCGAGAGAA+CGG - chr1_1:76656068-76656087 None:intergenic 45.0%
AGCAGGAACATAGAGAGAAG+CGG - chr1_1:76656103-76656122 None:intergenic 45.0%
TGAGGATCTTGAAGAGTAGC+AGG - chr1_1:76656120-76656139 None:intergenic 45.0%
ATATCGACGAGAACGTGATG+AGG - chr1_1:76656138-76656157 None:intergenic 45.0%
CATCACGTTCTCGTCGATAT+CGG + chr1_1:76656138-76656157 Msa0044990:CDS 45.0%
GAAAACCATGCCTGAAGGAA+AGG + chr1_1:76656292-76656311 Msa0044990:intron 45.0%
GTAATCCTTTCCTTCAGGCA+TGG - chr1_1:76656300-76656319 None:intergenic 45.0%
TCGCAGTAATCCTTTCCTTC+AGG - chr1_1:76656305-76656324 None:intergenic 45.0%
AAAAACGTTGCGGATGAAGC+TGG + chr1_1:76656699-76656718 Msa0044990:CDS 45.0%
AAAACGTTGCGGATGAAGCT+GGG + chr1_1:76656700-76656719 Msa0044990:CDS 45.0%
AGGAGGGATCTTGTGATTGT+TGG + chr1_1:76657068-76657087 Msa0044990:three_prime_UTR 45.0%
GGAGGGATCTTGTGATTGTT+GGG + chr1_1:76657069-76657088 Msa0044990:three_prime_UTR 45.0%
TGTTAGGCTCAGTCGAAGAT+AGG + chr1_1:76657197-76657216 Msa0044990:three_prime_UTR 45.0%
GTTAGGCTCAGTCGAAGATA+GGG + chr1_1:76657198-76657217 Msa0044990:three_prime_UTR 45.0%
TTAGGCTCAGTCGAAGATAG+GGG + chr1_1:76657199-76657218 Msa0044990:three_prime_UTR 45.0%
!!! ATAAGTAAAATAATTTATTT+TGG + chr1_1:76655730-76655749 Msa0044990:five_prime_UTR 5.0%
!!! ATAAGTAAAATAATTTATTT+TGG + chr1_1:76655730-76655749 Msa0044990:five_prime_UTR 5.0%
AATCAAATCGGAGCTGGAGG+AGG + chr1_1:76655820-76655839 Msa0044990:five_prime_UTR 50.0%
ATCAAATCGGAGCTGGAGGA+GGG + chr1_1:76655821-76655840 Msa0044990:five_prime_UTR 50.0%
GAGAGAGAGATCGTTGAGAG+CGG - chr1_1:76656046-76656065 None:intergenic 50.0%
GATCTCTCTCTCCGTTCTCT+CGG + chr1_1:76656054-76656073 Msa0044990:CDS 50.0%
ACATAGAGAGAAGCGGTGAG+AGG - chr1_1:76656096-76656115 None:intergenic 50.0%
GTTCTCGTCGATATCGGAAC+TGG + chr1_1:76656144-76656163 Msa0044990:CDS 50.0%
! CGGAACTGGTTATTTCGTCG+AGG + chr1_1:76656158-76656177 Msa0044990:CDS 50.0%
AATCAAATCGGAGCTGGAGG+AGG + chr1_1:76655820-76655839 Msa0044990:five_prime_UTR 50.0%
ATCAAATCGGAGCTGGAGGA+GGG + chr1_1:76655821-76655840 Msa0044990:five_prime_UTR 50.0%
GAGAGAGAGATCGTTGAGAG+CGG - chr1_1:76656046-76656065 None:intergenic 50.0%
GATCTCTCTCTCCGTTCTCT+CGG + chr1_1:76656054-76656073 Msa0044990:CDS 50.0%
ACATAGAGAGAAGCGGTGAG+AGG - chr1_1:76656096-76656115 None:intergenic 50.0%
GTTCTCGTCGATATCGGAAC+TGG + chr1_1:76656144-76656163 Msa0044990:CDS 50.0%
! CGGAACTGGTTATTTCGTCG+AGG + chr1_1:76656158-76656177 Msa0044990:CDS 50.0%
GAGTTCAAGACGAGTGGTGG+CGG - chr1_1:76656016-76656035 None:intergenic 55.0%
AGAGAGATCGTTGAGAGCGG+TGG - chr1_1:76656043-76656062 None:intergenic 55.0%
GAGAAGCGGTGAGAGGAACA+AGG - chr1_1:76656089-76656108 None:intergenic 55.0%
GAGTTCAAGACGAGTGGTGG+CGG - chr1_1:76656016-76656035 None:intergenic 55.0%
AGAGAGATCGTTGAGAGCGG+TGG - chr1_1:76656043-76656062 None:intergenic 55.0%
GAGAAGCGGTGAGAGGAACA+AGG - chr1_1:76656089-76656108 None:intergenic 55.0%
TCGGAGCTGGAGGAGGGTTT+AGG + chr1_1:76655827-76655846 Msa0044990:five_prime_UTR 60.0%
CGGAGCTGGAGGAGGGTTTA+GGG + chr1_1:76655828-76655847 Msa0044990:five_prime_UTR 60.0%
GCTTCGTCGAAGAGCGGAAG+CGG + chr1_1:76655886-76655905 Msa0044990:CDS 60.0%
GGCGAGTTCAAGACGAGTGG+TGG - chr1_1:76656019-76656038 None:intergenic 60.0%
GGTGGCGAGTTCAAGACGAG+TGG - chr1_1:76656022-76656041 None:intergenic 60.0%
GAGATCGTTGAGAGCGGTGG+TGG - chr1_1:76656040-76656059 None:intergenic 60.0%
TCGGAGCTGGAGGAGGGTTT+AGG + chr1_1:76655827-76655846 Msa0044990:five_prime_UTR 60.0%
CGGAGCTGGAGGAGGGTTTA+GGG + chr1_1:76655828-76655847 Msa0044990:five_prime_UTR 60.0%
GCTTCGTCGAAGAGCGGAAG+CGG + chr1_1:76655886-76655905 Msa0044990:CDS 60.0%
GGCGAGTTCAAGACGAGTGG+TGG - chr1_1:76656019-76656038 None:intergenic 60.0%
GGTGGCGAGTTCAAGACGAG+TGG - chr1_1:76656022-76656041 None:intergenic 60.0%
GAGATCGTTGAGAGCGGTGG+TGG - chr1_1:76656040-76656059 None:intergenic 60.0%
CGGAAGCGGAGCGATGACTC+TGG + chr1_1:76655900-76655919 Msa0044990:CDS 65.0%
! GCGGAGCGATGACTCTGGAG+AGG + chr1_1:76655905-76655924 Msa0044990:CDS 65.0%
CGGAAGCGGAGCGATGACTC+TGG + chr1_1:76655900-76655919 Msa0044990:CDS 65.0%
! GCGGAGCGATGACTCTGGAG+AGG + chr1_1:76655905-76655924 Msa0044990:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 76655709 76657249 76655709 ID=Msa0044990;Name=Msa0044990
chr1_1 mRNA 76655709 76657249 76655709 ID=Msa0044990-mRNA-1;Parent=Msa0044990;Name=Msa0044990-mRNA-1;_AED=0.01;_eAED=0.01;_QI=174|1|1|1|1|1|3|482|152
chr1_1 exon 76655709 76656179 76655709 ID=Msa0044990-mRNA-1:exon:26044;Parent=Msa0044990-mRNA-1
chr1_1 exon 76656293 76656370 76656293 ID=Msa0044990-mRNA-1:exon:26045;Parent=Msa0044990-mRNA-1
chr1_1 exon 76656684 76657249 76656684 ID=Msa0044990-mRNA-1:exon:26046;Parent=Msa0044990-mRNA-1
chr1_1 five_prime_UTR 76655709 76655882 76655709 ID=Msa0044990-mRNA-1:five_prime_utr;Parent=Msa0044990-mRNA-1
chr1_1 CDS 76655883 76656179 76655883 ID=Msa0044990-mRNA-1:cds;Parent=Msa0044990-mRNA-1
chr1_1 CDS 76656293 76656370 76656293 ID=Msa0044990-mRNA-1:cds;Parent=Msa0044990-mRNA-1
chr1_1 CDS 76656684 76656767 76656684 ID=Msa0044990-mRNA-1:cds;Parent=Msa0044990-mRNA-1
chr1_1 three_prime_UTR 76656768 76657249 76656768 ID=Msa0044990-mRNA-1:three_prime_utr;Parent=Msa0044990-mRNA-1
Gene Sequence

>Msa0044990

ATGGCTTCGTCGAAGAGCGGAAGCGGAGCGATGACTCTGGAGAGGATGAGCGTTGAACAGTTGAAAGCAGTTAAGGAACAAGCAGATATGGAAGTTAACCTTCTTCAAGACAGTCTCAACAATATCGGCACCGCCACCACTCGTCTTGAACTCGCCACCACCGCTCTCAACGATCTCTCTCTCCGTTCTCTCGGCAGTAAAATCCTTGTTCCTCTCACCGCTTCTCTCTATGTTCCTGCTACTCTTCAAGATCCTCATCACGTTCTCGTCGATATCGGAACTGGTTATTTCGTCGAGAAAACCATGCCTGAAGGAAAGGATTACTGCGAGCGCAAAATCAACTTACTCAAATCTAACTTCGATCAACTTGTTGAGGTTGCGTCCAAGAAGAAAAACGTTGCGGATGAAGCTGGGCTTATTTTACAGGCAAAATTGAAGCAATTAGCATCTTCATCCTAA
Protein sequence

>Msa0044990

MASSKSGSGAMTLERMSVEQLKAVKEQADMEVNLLQDSLNNIGTATTRLELATTALNDLSLRSLGSKILVPLTASLYVPATLQDPHHVLVDIGTGYFVEKTMPEGKDYCERKINLLKSNFDQLVEVASKKKNVADEAGLILQAKLKQLASSS*