AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0115870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115870 MtrunA17_Chr1g0211971 99.342 152 1 0 1 152 1 152 6.42e-109 306
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115870 AT5G23290.1 72.368 152 39 2 1 152 1 149 2.91e-73 216

Find 71 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTCTAAGTGCTCTTTAT+TGG 0.153990 1_3:-34769852 Msa0115870:three_prime_UTR
AAACGAATTCTTTCAATAAA+AGG 0.178416 1_3:+34770106 None:intergenic
ATAAGTAAAATAATTTATTT+TGG 0.191605 1_3:-34771250 Msa0115870:five_prime_UTR
AATGGCATTCGAGATTTAAA+AGG 0.248481 1_3:-34770016 Msa0115870:three_prime_UTR
TCGGAGCTGGAGGAGGGTTT+AGG 0.263067 1_3:-34771153 Msa0115870:five_prime_UTR
AGAACATTAGTTTGGACTAT+TGG 0.267818 1_3:-34770066 Msa0115870:three_prime_UTR
ATTCTATACCAGCTAACTTT+AGG 0.287756 1_3:+34770217 None:intergenic
CCTTTACTATATAATATTGT+AGG 0.292567 1_3:-34769829 Msa0115870:three_prime_UTR
GAGAAAATGGTGAGTAAAAT+TGG 0.295052 1_3:+34769883 None:intergenic
AGCACTCCATGCTCTTTATT+AGG 0.330031 1_3:-34769932 Msa0115870:three_prime_UTR
TCATTTCCTCATTTGATGTT+AGG 0.333820 1_3:-34769799 Msa0115870:three_prime_UTR
CATCACGTTCTCGTCGATAT+CGG 0.346200 1_3:-34770842 Msa0115870:CDS
AGTGTGTAAAAGAGAGAAAA+TGG 0.346336 1_3:+34769870 None:intergenic
CGGAGCTGGAGGAGGGTTTA+GGG 0.346867 1_3:-34771152 Msa0115870:five_prime_UTR
GATGCCTCAGAACATTAGTT+TGG 0.367543 1_3:-34770074 Msa0115870:three_prime_UTR
TGTTAGGCTCAGTCGAAGAT+AGG 0.389749 1_3:-34769783 Msa0115870:three_prime_UTR
AGTTAAGGAACAAGCAGATA+TGG 0.392343 1_3:-34771029 Msa0115870:CDS
GAGAGGGGCGATTAAATATC+AGG 0.394744 1_3:+34769748 None:intergenic
TCGCAGTAATCCTTTCCTTC+AGG 0.397139 1_3:+34770678 None:intergenic
GGAGGGATCTTGTGATTGTT+GGG 0.397497 1_3:-34769911 Msa0115870:three_prime_UTR
AGGAGGGATCTTGTGATTGT+TGG 0.405237 1_3:-34769912 Msa0115870:three_prime_UTR
TATTGTAATTGTAATCAAAT+CGG 0.414828 1_3:-34771172 Msa0115870:five_prime_UTR
TGAACAGTTGAAAGCAGTTA+AGG 0.432363 1_3:-34771044 Msa0115870:CDS
GATCTCTCTCTCCGTTCTCT+CGG 0.450391 1_3:-34770926 Msa0115870:CDS
CGGAAGCGGAGCGATGACTC+TGG 0.463988 1_3:-34771080 Msa0115870:CDS
CAAGACAGTCTCAACAATAT+CGG 0.466377 1_3:-34770992 Msa0115870:CDS
GCGATTAAATATCAGGAGTC+AGG 0.479053 1_3:+34769755 None:intergenic
TGTTGAGACTGTCTTGAAGA+AGG 0.480129 1_3:+34770999 None:intergenic
GTTAGGCTCAGTCGAAGATA+GGG 0.485542 1_3:-34769782 Msa0115870:three_prime_UTR
AATCAAATCGGAGCTGGAGG+AGG 0.507340 1_3:-34771160 Msa0115870:five_prime_UTR
ATCTTCATCCTAAAGTTAGC+TGG 0.507912 1_3:-34770225 Msa0115870:exon
GTTCTCGTCGATATCGGAAC+TGG 0.508671 1_3:-34770836 Msa0115870:CDS
TGAGGATCTTGAAGAGTAGC+AGG 0.512402 1_3:+34770863 None:intergenic
GAGATCGTTGAGAGCGGTGG+TGG 0.515505 1_3:+34770943 None:intergenic
CATAACACTGGTGTTAGAGT+TGG 0.520800 1_3:-34770150 Msa0115870:three_prime_UTR
ATGGTACAAATACAACGCCT+TGG 0.522364 1_3:+34770180 None:intergenic
CTCCATGCTCTTTATTAGGA+GGG 0.522506 1_3:-34769928 Msa0115870:three_prime_UTR
GAAAACCATGCCTGAAGGAA+AGG 0.522733 1_3:-34770688 Msa0115870:CDS
GCTTCGTCGAAGAGCGGAAG+CGG 0.528323 1_3:-34771094 Msa0115870:CDS
GAGGTACTAAAATAATAAAG+TGG 0.530105 1_3:-34771226 Msa0115870:five_prime_UTR
AATTGTAATCAAATCGGAGC+TGG 0.532751 1_3:-34771166 Msa0115870:five_prime_UTR
AAAACGTTGCGGATGAAGCT+GGG 0.534177 1_3:-34770280 Msa0115870:CDS
GTAATCCTTTCCTTCAGGCA+TGG 0.536343 1_3:+34770683 None:intergenic
TAGAAAATCTAGTTGACCAA+TGG 0.538247 1_3:-34770034 Msa0115870:three_prime_UTR
ATCCCTCCTAATAAAGAGCA+TGG 0.538472 1_3:+34769926 None:intergenic
CCTACAATATTATATAGTAA+AGG 0.541243 1_3:+34769829 None:intergenic
ACTCCATGCTCTTTATTAGG+AGG 0.542891 1_3:-34769929 Msa0115870:three_prime_UTR
TAACTTCGATCAACTTGTTG+AGG 0.544631 1_3:-34770631 Msa0115870:intron
GAGTTCAAGACGAGTGGTGG+CGG 0.544654 1_3:+34770967 None:intergenic
ACACAAGTCTTAATCAGAGC+AGG 0.545280 1_3:-34769961 Msa0115870:three_prime_UTR
CGAGATTTAAAAGGTTAATG+TGG 0.550201 1_3:-34770007 Msa0115870:three_prime_UTR
GGCGAGTTCAAGACGAGTGG+TGG 0.560196 1_3:+34770964 None:intergenic
GTTAATGTGGAAATAAGCAT+TGG 0.560930 1_3:-34769994 Msa0115870:three_prime_UTR
AGAGAGATCGTTGAGAGCGG+TGG 0.573255 1_3:+34770940 None:intergenic
ACTGAGCCTAACATCAAATG+AGG 0.577162 1_3:+34769793 None:intergenic
ATCAAATCGGAGCTGGAGGA+GGG 0.591866 1_3:-34771159 Msa0115870:five_prime_UTR
TGTAATCAAATCGGAGCTGG+AGG 0.598285 1_3:-34771163 Msa0115870:five_prime_UTR
GAGAAGCGGTGAGAGGAACA+AGG 0.602439 1_3:+34770894 None:intergenic
AAAATGGCTTCGTCGAAGAG+CGG 0.608939 1_3:-34771100 Msa0115870:CDS
ATATCGACGAGAACGTGATG+AGG 0.609043 1_3:+34770845 None:intergenic
ACATAGAGAGAAGCGGTGAG+AGG 0.615765 1_3:+34770887 None:intergenic
AAAATGGTGAGTAAAATTGG+TGG 0.618170 1_3:+34769886 None:intergenic
AAACAGAAAACCATGCCTGA+AGG 0.621995 1_3:-34770693 Msa0115870:intron
ACCATAGAAAATCATAACAC+TGG 0.623201 1_3:-34770162 Msa0115870:three_prime_UTR
AGCAGGAACATAGAGAGAAG+CGG 0.640463 1_3:+34770880 None:intergenic
GCGGAGCGATGACTCTGGAG+AGG 0.643180 1_3:-34771075 Msa0115870:CDS
TAGTCCAAACTAATGTTCTG+AGG 0.643856 1_3:+34770070 None:intergenic
TTAGGCTCAGTCGAAGATAG+GGG 0.656337 1_3:-34769781 Msa0115870:three_prime_UTR
GAGAGAGAGATCGTTGAGAG+CGG 0.661545 1_3:+34770937 None:intergenic
CGGAACTGGTTATTTCGTCG+AGG 0.673821 1_3:-34770822 Msa0115870:intron
GGTGGCGAGTTCAAGACGAG+TGG 0.675476 1_3:+34770961 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATAATTTATTTTGGTG+AGG - chr1_3:34769779-34769798 Msa0115870:three_prime_UTR 15.0%
!! TATTGTAATTGTAATCAAAT+CGG - chr1_3:34769852-34769871 Msa0115870:three_prime_UTR 15.0%
!! ACAGATCTAAATAAATTAAT+CGG + chr1_3:34770245-34770264 None:intergenic 15.0%
!!! TTAAACTTAATTGCAATTTT+CGG + chr1_3:34769755-34769774 None:intergenic 15.0%
!!! TAAAATAATTTATTTTGGTG+AGG - chr1_3:34769779-34769798 Msa0115870:three_prime_UTR 15.0%
!! TATTGTAATTGTAATCAAAT+CGG - chr1_3:34769852-34769871 Msa0115870:three_prime_UTR 15.0%
!! ACAGATCTAAATAAATTAAT+CGG + chr1_3:34770245-34770264 None:intergenic 15.0%
!! AGATCTAAATAAATTAATCG+GGG + chr1_3:34770243-34770262 None:intergenic 20.0%
!! CAGATCTAAATAAATTAATC+GGG + chr1_3:34770244-34770263 None:intergenic 20.0%
!! CTAGACAAAATATCAAATTA+GGG + chr1_3:34770615-34770634 None:intergenic 20.0%
!! AAAAATATCAAAGAGAAAAC+TGG + chr1_3:34770650-34770669 None:intergenic 20.0%
!!! AATTCAATTACTTTTTTCCA+AGG - chr1_3:34770827-34770846 Msa0115870:CDS 20.0%
!! AAACGAATTCTTTCAATAAA+AGG + chr1_3:34770921-34770940 None:intergenic 20.0%
!! CCTACAATATTATATAGTAA+AGG + chr1_3:34771198-34771217 None:intergenic 20.0%
!! CCTTTACTATATAATATTGT+AGG - chr1_3:34771195-34771214 Msa0115870:five_prime_UTR 20.0%
!!! TAAACTTAATTGCAATTTTC+GGG + chr1_3:34769754-34769773 None:intergenic 20.0%
!! AGATCTAAATAAATTAATCG+GGG + chr1_3:34770243-34770262 None:intergenic 20.0%
!! CAGATCTAAATAAATTAATC+GGG + chr1_3:34770244-34770263 None:intergenic 20.0%
!! CTAGACAAAATATCAAATTA+GGG + chr1_3:34770615-34770634 None:intergenic 20.0%
!! AAAAATATCAAAGAGAAAAC+TGG + chr1_3:34770650-34770669 None:intergenic 20.0%
!!! AATTCAATTACTTTTTTCCA+AGG - chr1_3:34770827-34770846 Msa0115870:CDS 20.0%
!! AAACGAATTCTTTCAATAAA+AGG + chr1_3:34770921-34770940 None:intergenic 20.0%
!! CCTACAATATTATATAGTAA+AGG + chr1_3:34771198-34771217 None:intergenic 20.0%
!! CCTTTACTATATAATATTGT+AGG - chr1_3:34771195-34771214 Msa0115870:five_prime_UTR 20.0%
!! GAGGTACTAAAATAATAAAG+TGG - chr1_3:34769798-34769817 Msa0115870:three_prime_UTR 25.0%
!! TATCGTATATGCTTCTTTTT+AGG - chr1_3:34770264-34770283 Msa0115870:CDS 25.0%
!! ATCGTATATGCTTCTTTTTA+GGG - chr1_3:34770265-34770284 Msa0115870:CDS 25.0%
!!! GTGTTAGTTTTATACTAATG+TGG - chr1_3:34770538-34770557 Msa0115870:intron 25.0%
! CCTAGACAAAATATCAAATT+AGG + chr1_3:34770616-34770635 None:intergenic 25.0%
!! CCTAATTTGATATTTTGTCT+AGG - chr1_3:34770613-34770632 Msa0115870:intron 25.0%
!! AAACTTAATTGCAATTTTCG+GGG + chr1_3:34769753-34769772 None:intergenic 25.0%
!! GAGGTACTAAAATAATAAAG+TGG - chr1_3:34769798-34769817 Msa0115870:three_prime_UTR 25.0%
!! TATCGTATATGCTTCTTTTT+AGG - chr1_3:34770264-34770283 Msa0115870:CDS 25.0%
!! ATCGTATATGCTTCTTTTTA+GGG - chr1_3:34770265-34770284 Msa0115870:CDS 25.0%
!!! GTGTTAGTTTTATACTAATG+TGG - chr1_3:34770538-34770557 Msa0115870:intron 25.0%
! CCTAGACAAAATATCAAATT+AGG + chr1_3:34770616-34770635 None:intergenic 25.0%
!! CCTAATTTGATATTTTGTCT+AGG - chr1_3:34770613-34770632 Msa0115870:intron 25.0%
AATCGAGAACCTTCAAAAAA+TGG - chr1_3:34769908-34769927 Msa0115870:three_prime_UTR 30.0%
!!! TATGCTTCTTTTTAGGGTTA+TGG - chr1_3:34770271-34770290 Msa0115870:CDS 30.0%
CATGATGTAATTACAAATGC+AGG - chr1_3:34770706-34770725 Msa0115870:intron 30.0%
ATTCTATACCAGCTAACTTT+AGG + chr1_3:34770810-34770829 None:intergenic 30.0%
ACCATAGAAAATCATAACAC+TGG - chr1_3:34770862-34770881 Msa0115870:CDS 30.0%
! ACCAGTGTTATGATTTTCTA+TGG + chr1_3:34770866-34770885 None:intergenic 30.0%
! AGAACATTAGTTTGGACTAT+TGG - chr1_3:34770958-34770977 Msa0115870:CDS 30.0%
TAGAAAATCTAGTTGACCAA+TGG - chr1_3:34770990-34771009 Msa0115870:CDS 30.0%
! TTTTAAATCTCGAATGCCAT+TGG + chr1_3:34771009-34771028 None:intergenic 30.0%
AATGGCATTCGAGATTTAAA+AGG - chr1_3:34771008-34771027 Msa0115870:CDS 30.0%
CGAGATTTAAAAGGTTAATG+TGG - chr1_3:34771017-34771036 Msa0115870:CDS 30.0%
GTTAATGTGGAAATAAGCAT+TGG - chr1_3:34771030-34771049 Msa0115870:CDS 30.0%
AAAATGGTGAGTAAAATTGG+TGG + chr1_3:34771141-34771160 None:intergenic 30.0%
GAGAAAATGGTGAGTAAAAT+TGG + chr1_3:34771144-34771163 None:intergenic 30.0%
AGTGTGTAAAAGAGAGAAAA+TGG + chr1_3:34771157-34771176 None:intergenic 30.0%
TCATTTCCTCATTTGATGTT+AGG - chr1_3:34771225-34771244 Msa0115870:five_prime_UTR 30.0%
AATCGAGAACCTTCAAAAAA+TGG - chr1_3:34769908-34769927 Msa0115870:three_prime_UTR 30.0%
!!! TATGCTTCTTTTTAGGGTTA+TGG - chr1_3:34770271-34770290 Msa0115870:CDS 30.0%
CATGATGTAATTACAAATGC+AGG - chr1_3:34770706-34770725 Msa0115870:intron 30.0%
ATTCTATACCAGCTAACTTT+AGG + chr1_3:34770810-34770829 None:intergenic 30.0%
ACCATAGAAAATCATAACAC+TGG - chr1_3:34770862-34770881 Msa0115870:CDS 30.0%
! ACCAGTGTTATGATTTTCTA+TGG + chr1_3:34770866-34770885 None:intergenic 30.0%
! AGAACATTAGTTTGGACTAT+TGG - chr1_3:34770958-34770977 Msa0115870:CDS 30.0%
TAGAAAATCTAGTTGACCAA+TGG - chr1_3:34770990-34771009 Msa0115870:CDS 30.0%
! TTTTAAATCTCGAATGCCAT+TGG + chr1_3:34771009-34771028 None:intergenic 30.0%
AATGGCATTCGAGATTTAAA+AGG - chr1_3:34771008-34771027 Msa0115870:CDS 30.0%
CGAGATTTAAAAGGTTAATG+TGG - chr1_3:34771017-34771036 Msa0115870:CDS 30.0%
GTTAATGTGGAAATAAGCAT+TGG - chr1_3:34771030-34771049 Msa0115870:CDS 30.0%
AAAATGGTGAGTAAAATTGG+TGG + chr1_3:34771141-34771160 None:intergenic 30.0%
GAGAAAATGGTGAGTAAAAT+TGG + chr1_3:34771144-34771163 None:intergenic 30.0%
AGTGTGTAAAAGAGAGAAAA+TGG + chr1_3:34771157-34771176 None:intergenic 30.0%
TCATTTCCTCATTTGATGTT+AGG - chr1_3:34771225-34771244 Msa0115870:five_prime_UTR 30.0%
AATTGTAATCAAATCGGAGC+TGG - chr1_3:34769858-34769877 Msa0115870:three_prime_UTR 35.0%
TGAACAGTTGAAAGCAGTTA+AGG - chr1_3:34769980-34769999 Msa0115870:three_prime_UTR 35.0%
AGTTAAGGAACAAGCAGATA+TGG - chr1_3:34769995-34770014 Msa0115870:three_prime_UTR 35.0%
CAAGACAGTCTCAACAATAT+CGG - chr1_3:34770032-34770051 Msa0115870:three_prime_UTR 35.0%
!! TAACTTCGATCAACTTGTTG+AGG - chr1_3:34770393-34770412 Msa0115870:intron 35.0%
CTAATGTGGTTATTCTGTTG+TGG - chr1_3:34770552-34770571 Msa0115870:intron 35.0%
ATCTTCATCCTAAAGTTAGC+TGG - chr1_3:34770799-34770818 Msa0115870:intron 35.0%
TAGTCCAAACTAATGTTCTG+AGG + chr1_3:34770957-34770976 None:intergenic 35.0%
ACACTCTAAGTGCTCTTTAT+TGG - chr1_3:34771172-34771191 Msa0115870:five_prime_UTR 35.0%
AATTGTAATCAAATCGGAGC+TGG - chr1_3:34769858-34769877 Msa0115870:three_prime_UTR 35.0%
TGAACAGTTGAAAGCAGTTA+AGG - chr1_3:34769980-34769999 Msa0115870:three_prime_UTR 35.0%
AGTTAAGGAACAAGCAGATA+TGG - chr1_3:34769995-34770014 Msa0115870:three_prime_UTR 35.0%
CAAGACAGTCTCAACAATAT+CGG - chr1_3:34770032-34770051 Msa0115870:three_prime_UTR 35.0%
!! TAACTTCGATCAACTTGTTG+AGG - chr1_3:34770393-34770412 Msa0115870:intron 35.0%
CTAATGTGGTTATTCTGTTG+TGG - chr1_3:34770552-34770571 Msa0115870:intron 35.0%
ATCTTCATCCTAAAGTTAGC+TGG - chr1_3:34770799-34770818 Msa0115870:intron 35.0%
TAGTCCAAACTAATGTTCTG+AGG + chr1_3:34770957-34770976 None:intergenic 35.0%
ACACTCTAAGTGCTCTTTAT+TGG - chr1_3:34771172-34771191 Msa0115870:five_prime_UTR 35.0%
!!! TCGACGAAGCCATTTTTTGA+AGG + chr1_3:34769920-34769939 None:intergenic 40.0%
TGTTGAGACTGTCTTGAAGA+AGG + chr1_3:34770028-34770047 None:intergenic 40.0%
AAACAGAAAACCATGCCTGA+AGG - chr1_3:34770331-34770350 Msa0115870:intron 40.0%
GTCCAAGAAGAAAAACGTTG+CGG - chr1_3:34770733-34770752 Msa0115870:intron 40.0%
! ATCCGCAACGTTTTTCTTCT+TGG + chr1_3:34770738-34770757 None:intergenic 40.0%
!!! TGAAGCTGGGCTTATTTTAC+AGG - chr1_3:34770757-34770776 Msa0115870:intron 40.0%
ATGGTACAAATACAACGCCT+TGG + chr1_3:34770847-34770866 None:intergenic 40.0%
! CATAACACTGGTGTTAGAGT+TGG - chr1_3:34770874-34770893 Msa0115870:CDS 40.0%
GATGCCTCAGAACATTAGTT+TGG - chr1_3:34770950-34770969 Msa0115870:CDS 40.0%
ACACAAGTCTTAATCAGAGC+AGG - chr1_3:34771063-34771082 Msa0115870:CDS 40.0%
AGCACTCCATGCTCTTTATT+AGG - chr1_3:34771092-34771111 Msa0115870:CDS 40.0%
ACTCCATGCTCTTTATTAGG+AGG - chr1_3:34771095-34771114 Msa0115870:CDS 40.0%
CTCCATGCTCTTTATTAGGA+GGG - chr1_3:34771096-34771115 Msa0115870:CDS 40.0%
ATCCCTCCTAATAAAGAGCA+TGG + chr1_3:34771101-34771120 None:intergenic 40.0%
ACTGAGCCTAACATCAAATG+AGG + chr1_3:34771234-34771253 None:intergenic 40.0%
GCGATTAAATATCAGGAGTC+AGG + chr1_3:34771272-34771291 None:intergenic 40.0%
!!! TCGACGAAGCCATTTTTTGA+AGG + chr1_3:34769920-34769939 None:intergenic 40.0%
TGTTGAGACTGTCTTGAAGA+AGG + chr1_3:34770028-34770047 None:intergenic 40.0%
AAACAGAAAACCATGCCTGA+AGG - chr1_3:34770331-34770350 Msa0115870:intron 40.0%
GTCCAAGAAGAAAAACGTTG+CGG - chr1_3:34770733-34770752 Msa0115870:intron 40.0%
! ATCCGCAACGTTTTTCTTCT+TGG + chr1_3:34770738-34770757 None:intergenic 40.0%
!!! TGAAGCTGGGCTTATTTTAC+AGG - chr1_3:34770757-34770776 Msa0115870:intron 40.0%
ATGGTACAAATACAACGCCT+TGG + chr1_3:34770847-34770866 None:intergenic 40.0%
! CATAACACTGGTGTTAGAGT+TGG - chr1_3:34770874-34770893 Msa0115870:CDS 40.0%
GATGCCTCAGAACATTAGTT+TGG - chr1_3:34770950-34770969 Msa0115870:CDS 40.0%
ACACAAGTCTTAATCAGAGC+AGG - chr1_3:34771063-34771082 Msa0115870:CDS 40.0%
AGCACTCCATGCTCTTTATT+AGG - chr1_3:34771092-34771111 Msa0115870:CDS 40.0%
ACTCCATGCTCTTTATTAGG+AGG - chr1_3:34771095-34771114 Msa0115870:CDS 40.0%
CTCCATGCTCTTTATTAGGA+GGG - chr1_3:34771096-34771115 Msa0115870:CDS 40.0%
ATCCCTCCTAATAAAGAGCA+TGG + chr1_3:34771101-34771120 None:intergenic 40.0%
ACTGAGCCTAACATCAAATG+AGG + chr1_3:34771234-34771253 None:intergenic 40.0%
GCGATTAAATATCAGGAGTC+AGG + chr1_3:34771272-34771291 None:intergenic 40.0%
TGTAATCAAATCGGAGCTGG+AGG - chr1_3:34769861-34769880 Msa0115870:three_prime_UTR 45.0%
AAAATGGCTTCGTCGAAGAG+CGG - chr1_3:34769924-34769943 Msa0115870:three_prime_UTR 45.0%
! GGATTTTACTGCCGAGAGAA+CGG + chr1_3:34770112-34770131 None:intergenic 45.0%
AGCAGGAACATAGAGAGAAG+CGG + chr1_3:34770147-34770166 None:intergenic 45.0%
TGAGGATCTTGAAGAGTAGC+AGG + chr1_3:34770164-34770183 None:intergenic 45.0%
ATATCGACGAGAACGTGATG+AGG + chr1_3:34770182-34770201 None:intergenic 45.0%
CATCACGTTCTCGTCGATAT+CGG - chr1_3:34770182-34770201 Msa0115870:three_prime_UTR 45.0%
GAAAACCATGCCTGAAGGAA+AGG - chr1_3:34770336-34770355 Msa0115870:intron 45.0%
GTAATCCTTTCCTTCAGGCA+TGG + chr1_3:34770344-34770363 None:intergenic 45.0%
TCGCAGTAATCCTTTCCTTC+AGG + chr1_3:34770349-34770368 None:intergenic 45.0%
AAAAACGTTGCGGATGAAGC+TGG - chr1_3:34770743-34770762 Msa0115870:intron 45.0%
AAAACGTTGCGGATGAAGCT+GGG - chr1_3:34770744-34770763 Msa0115870:intron 45.0%
AGGAGGGATCTTGTGATTGT+TGG - chr1_3:34771112-34771131 Msa0115870:exon 45.0%
GGAGGGATCTTGTGATTGTT+GGG - chr1_3:34771113-34771132 Msa0115870:exon 45.0%
TGTTAGGCTCAGTCGAAGAT+AGG - chr1_3:34771241-34771260 Msa0115870:five_prime_UTR 45.0%
GTTAGGCTCAGTCGAAGATA+GGG - chr1_3:34771242-34771261 Msa0115870:five_prime_UTR 45.0%
TTAGGCTCAGTCGAAGATAG+GGG - chr1_3:34771243-34771262 Msa0115870:five_prime_UTR 45.0%
TGTAATCAAATCGGAGCTGG+AGG - chr1_3:34769861-34769880 Msa0115870:three_prime_UTR 45.0%
AAAATGGCTTCGTCGAAGAG+CGG - chr1_3:34769924-34769943 Msa0115870:three_prime_UTR 45.0%
! GGATTTTACTGCCGAGAGAA+CGG + chr1_3:34770112-34770131 None:intergenic 45.0%
AGCAGGAACATAGAGAGAAG+CGG + chr1_3:34770147-34770166 None:intergenic 45.0%
TGAGGATCTTGAAGAGTAGC+AGG + chr1_3:34770164-34770183 None:intergenic 45.0%
ATATCGACGAGAACGTGATG+AGG + chr1_3:34770182-34770201 None:intergenic 45.0%
CATCACGTTCTCGTCGATAT+CGG - chr1_3:34770182-34770201 Msa0115870:three_prime_UTR 45.0%
GAAAACCATGCCTGAAGGAA+AGG - chr1_3:34770336-34770355 Msa0115870:intron 45.0%
GTAATCCTTTCCTTCAGGCA+TGG + chr1_3:34770344-34770363 None:intergenic 45.0%
TCGCAGTAATCCTTTCCTTC+AGG + chr1_3:34770349-34770368 None:intergenic 45.0%
AAAAACGTTGCGGATGAAGC+TGG - chr1_3:34770743-34770762 Msa0115870:intron 45.0%
AAAACGTTGCGGATGAAGCT+GGG - chr1_3:34770744-34770763 Msa0115870:intron 45.0%
AGGAGGGATCTTGTGATTGT+TGG - chr1_3:34771112-34771131 Msa0115870:exon 45.0%
GGAGGGATCTTGTGATTGTT+GGG - chr1_3:34771113-34771132 Msa0115870:exon 45.0%
TGTTAGGCTCAGTCGAAGAT+AGG - chr1_3:34771241-34771260 Msa0115870:five_prime_UTR 45.0%
GTTAGGCTCAGTCGAAGATA+GGG - chr1_3:34771242-34771261 Msa0115870:five_prime_UTR 45.0%
TTAGGCTCAGTCGAAGATAG+GGG - chr1_3:34771243-34771262 Msa0115870:five_prime_UTR 45.0%
!!! ATAAGTAAAATAATTTATTT+TGG - chr1_3:34769774-34769793 Msa0115870:three_prime_UTR 5.0%
!!! ATAAGTAAAATAATTTATTT+TGG - chr1_3:34769774-34769793 Msa0115870:three_prime_UTR 5.0%
AATCAAATCGGAGCTGGAGG+AGG - chr1_3:34769864-34769883 Msa0115870:three_prime_UTR 50.0%
ATCAAATCGGAGCTGGAGGA+GGG - chr1_3:34769865-34769884 Msa0115870:three_prime_UTR 50.0%
GAGAGAGAGATCGTTGAGAG+CGG + chr1_3:34770090-34770109 None:intergenic 50.0%
GATCTCTCTCTCCGTTCTCT+CGG - chr1_3:34770098-34770117 Msa0115870:three_prime_UTR 50.0%
ACATAGAGAGAAGCGGTGAG+AGG + chr1_3:34770140-34770159 None:intergenic 50.0%
GTTCTCGTCGATATCGGAAC+TGG - chr1_3:34770188-34770207 Msa0115870:three_prime_UTR 50.0%
! CGGAACTGGTTATTTCGTCG+AGG - chr1_3:34770202-34770221 Msa0115870:three_prime_UTR 50.0%
AATCAAATCGGAGCTGGAGG+AGG - chr1_3:34769864-34769883 Msa0115870:three_prime_UTR 50.0%
ATCAAATCGGAGCTGGAGGA+GGG - chr1_3:34769865-34769884 Msa0115870:three_prime_UTR 50.0%
GAGAGAGAGATCGTTGAGAG+CGG + chr1_3:34770090-34770109 None:intergenic 50.0%
GATCTCTCTCTCCGTTCTCT+CGG - chr1_3:34770098-34770117 Msa0115870:three_prime_UTR 50.0%
ACATAGAGAGAAGCGGTGAG+AGG + chr1_3:34770140-34770159 None:intergenic 50.0%
GTTCTCGTCGATATCGGAAC+TGG - chr1_3:34770188-34770207 Msa0115870:three_prime_UTR 50.0%
! CGGAACTGGTTATTTCGTCG+AGG - chr1_3:34770202-34770221 Msa0115870:three_prime_UTR 50.0%
GAGTTCAAGACGAGTGGTGG+CGG + chr1_3:34770060-34770079 None:intergenic 55.0%
AGAGAGATCGTTGAGAGCGG+TGG + chr1_3:34770087-34770106 None:intergenic 55.0%
GAGAAGCGGTGAGAGGAACA+AGG + chr1_3:34770133-34770152 None:intergenic 55.0%
GAGTTCAAGACGAGTGGTGG+CGG + chr1_3:34770060-34770079 None:intergenic 55.0%
AGAGAGATCGTTGAGAGCGG+TGG + chr1_3:34770087-34770106 None:intergenic 55.0%
GAGAAGCGGTGAGAGGAACA+AGG + chr1_3:34770133-34770152 None:intergenic 55.0%
TCGGAGCTGGAGGAGGGTTT+AGG - chr1_3:34769871-34769890 Msa0115870:three_prime_UTR 60.0%
CGGAGCTGGAGGAGGGTTTA+GGG - chr1_3:34769872-34769891 Msa0115870:three_prime_UTR 60.0%
GCTTCGTCGAAGAGCGGAAG+CGG - chr1_3:34769930-34769949 Msa0115870:three_prime_UTR 60.0%
GGCGAGTTCAAGACGAGTGG+TGG + chr1_3:34770063-34770082 None:intergenic 60.0%
GGTGGCGAGTTCAAGACGAG+TGG + chr1_3:34770066-34770085 None:intergenic 60.0%
GAGATCGTTGAGAGCGGTGG+TGG + chr1_3:34770084-34770103 None:intergenic 60.0%
TCGGAGCTGGAGGAGGGTTT+AGG - chr1_3:34769871-34769890 Msa0115870:three_prime_UTR 60.0%
CGGAGCTGGAGGAGGGTTTA+GGG - chr1_3:34769872-34769891 Msa0115870:three_prime_UTR 60.0%
GCTTCGTCGAAGAGCGGAAG+CGG - chr1_3:34769930-34769949 Msa0115870:three_prime_UTR 60.0%
GGCGAGTTCAAGACGAGTGG+TGG + chr1_3:34770063-34770082 None:intergenic 60.0%
GGTGGCGAGTTCAAGACGAG+TGG + chr1_3:34770066-34770085 None:intergenic 60.0%
GAGATCGTTGAGAGCGGTGG+TGG + chr1_3:34770084-34770103 None:intergenic 60.0%
CGGAAGCGGAGCGATGACTC+TGG - chr1_3:34769944-34769963 Msa0115870:three_prime_UTR 65.0%
! GCGGAGCGATGACTCTGGAG+AGG - chr1_3:34769949-34769968 Msa0115870:three_prime_UTR 65.0%
CGGAAGCGGAGCGATGACTC+TGG - chr1_3:34769944-34769963 Msa0115870:three_prime_UTR 65.0%
! GCGGAGCGATGACTCTGGAG+AGG - chr1_3:34769949-34769968 Msa0115870:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 34769753 34771293 34769753 ID=Msa0115870;Name=Msa0115870
chr1_3 mRNA 34769753 34771293 34769753 ID=Msa0115870-mRNA-1;Parent=Msa0115870;Name=Msa0115870-mRNA-1;_AED=0.01;_eAED=0.01;_QI=174|1|1|1|1|1|3|482|152
chr1_3 exon 34769753 34770318 34769753 ID=Msa0115870-mRNA-1:exon:11012;Parent=Msa0115870-mRNA-1
chr1_3 exon 34770632 34770709 34770632 ID=Msa0115870-mRNA-1:exon:11011;Parent=Msa0115870-mRNA-1
chr1_3 exon 34770823 34771293 34770823 ID=Msa0115870-mRNA-1:exon:11010;Parent=Msa0115870-mRNA-1
chr1_3 five_prime_UTR 34771120 34771293 34771120 ID=Msa0115870-mRNA-1:five_prime_utr;Parent=Msa0115870-mRNA-1
chr1_3 CDS 34770823 34771119 34770823 ID=Msa0115870-mRNA-1:cds;Parent=Msa0115870-mRNA-1
chr1_3 CDS 34770632 34770709 34770632 ID=Msa0115870-mRNA-1:cds;Parent=Msa0115870-mRNA-1
chr1_3 CDS 34770235 34770318 34770235 ID=Msa0115870-mRNA-1:cds;Parent=Msa0115870-mRNA-1
chr1_3 three_prime_UTR 34769753 34770234 34769753 ID=Msa0115870-mRNA-1:three_prime_utr;Parent=Msa0115870-mRNA-1
Gene Sequence

>Msa0115870

ATGGCTTCGTCGAAGAGCGGAAGCGGAGCGATGACTCTGGAGAGGATGAGCGTTGAACAGTTGAAAGCAGTTAAGGAACAAGCAGATATGGAAGTTAACCTTCTTCAAGACAGTCTCAACAATATCGGCACCGCCACCACTCGTCTTGAACTCGCCACCACCGCTCTCAACGATCTCTCTCTCCGTTCTCTCGGCAGTAAAATCCTTGTTCCTCTCACCGCTTCTCTCTATGTTCCTGCTACTCTTCAAGATCCTCATCACGTTCTCGTCGATATCGGAACTGGTTATTTCGTCGAGAAAACCATGCCTGAAGGAAAGGATTACTGCGAGCGCAAAATCAACTTACTCAAATCTAACTTCGATCAACTTGTTGAGGTTGCGTCCAAGAAGAAAAACGTTGCGGATGAAGCTGGGCTTATTTTACAGGCAAAATTGAAGCAATTAGCATCTTCATCCTAA
Protein sequence

>Msa0115870

MASSKSGSGAMTLERMSVEQLKAVKEQADMEVNLLQDSLNNIGTATTRLELATTALNDLSLRSLGSKILVPLTASLYVPATLQDPHHVLVDIGTGYFVEKTMPEGKDYCERKINLLKSNFDQLVEVASKKKNVADEAGLILQAKLKQLASSS*