AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1420430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1420430 MtrunA17_Chr1g0211971 99.342 152 1 0 1 152 1 152 6.42e-109 306
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1420430 AT5G23290.1 72.368 152 39 2 1 152 1 149 2.91e-73 216

Find 66 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTCAAAGTGCTCTTTAT+TGG 0.161123 tig0024802:-44714 Msa1420430:three_prime_UTR
AAACGAATTCTTTCAATAAA+AGG 0.178416 tig0024802:+44968 None:intergenic
ATAAGTAAAATAATTTATTT+TGG 0.191605 tig0024802:-46112 Msa1420430:five_prime_UTR
AATGACATTCGAGATTTAAA+AGG 0.255512 tig0024802:-44878 Msa1420430:three_prime_UTR
TCGGAGCTGGAGGAGGGTTT+AGG 0.263067 tig0024802:-46015 Msa1420430:five_prime_UTR
AGAACATTAGTTTGGACTAT+TGG 0.267818 tig0024802:-44928 Msa1420430:three_prime_UTR
ATTCTATACCAGCTAACTTT+AGG 0.287756 tig0024802:+45079 None:intergenic
CCTTTACTATATAATATTGT+AGG 0.292567 tig0024802:-44691 Msa1420430:three_prime_UTR
GAGAAAATGGTGAGTAAAAT+TGG 0.295052 tig0024802:+44745 None:intergenic
TCATTTCCTCATTTGATGTT+AGG 0.333820 tig0024802:-44661 Msa1420430:three_prime_UTR
CATCACGTTCTCGTCGATAT+CGG 0.346200 tig0024802:-45704 Msa1420430:CDS
CGGAGCTGGAGGAGGGTTTA+GGG 0.346867 tig0024802:-46014 Msa1420430:five_prime_UTR
GATGCCTCAGAACATTAGTT+TGG 0.367543 tig0024802:-44936 Msa1420430:three_prime_UTR
TGTTAGGCTCAGTCGAAGAT+AGG 0.389749 tig0024802:-44645 Msa1420430:three_prime_UTR
AGTTAAGGAACAAGCAGATA+TGG 0.392343 tig0024802:-45891 Msa1420430:CDS
GAGAGGGGCGATTAAATATC+AGG 0.394744 tig0024802:+44610 None:intergenic
TCGCAGTAATCCTTTCCTTC+AGG 0.397139 tig0024802:+45540 None:intergenic
GGAGGGATCTTGTGATTGTT+GGG 0.397497 tig0024802:-44773 Msa1420430:three_prime_UTR
AGGAGGGATCTTGTGATTGT+TGG 0.405237 tig0024802:-44774 Msa1420430:three_prime_UTR
TATTGTAATTGTAATCAAAT+CGG 0.414828 tig0024802:-46034 Msa1420430:five_prime_UTR
TGAACAGTTGAAAGCAGTTA+AGG 0.432363 tig0024802:-45906 Msa1420430:CDS
AGTGTGTAAAAGAGAGAAAA+TGG 0.438240 tig0024802:+44732 None:intergenic
GATCTCTCTCTCCGTTCTCT+CGG 0.450391 tig0024802:-45788 Msa1420430:CDS
CGGAAGCGGAGCGATGACTC+TGG 0.463988 tig0024802:-45942 Msa1420430:CDS
CAAGACAGTCTCAACAATAT+CGG 0.466377 tig0024802:-45854 Msa1420430:CDS
GCGATTAAATATCAGGAGTC+AGG 0.479053 tig0024802:+44617 None:intergenic
TGTTGAGACTGTCTTGAAGA+AGG 0.480129 tig0024802:+45861 None:intergenic
GTTAGGCTCAGTCGAAGATA+GGG 0.485542 tig0024802:-44644 Msa1420430:three_prime_UTR
AATCAAATCGGAGCTGGAGG+AGG 0.507340 tig0024802:-46022 Msa1420430:five_prime_UTR
ATCTTCATCCTAAAGTTAGC+TGG 0.507912 tig0024802:-45087 Msa1420430:exon
GTTCTCGTCGATATCGGAAC+TGG 0.508671 tig0024802:-45698 Msa1420430:CDS
TGAGGATCTTGAAGAGTAGC+AGG 0.512402 tig0024802:+45725 None:intergenic
GAGATCGTTGAGAGCGGTGG+TGG 0.515505 tig0024802:+45805 None:intergenic
CATAACACTGGTGTTAGAGT+TGG 0.520800 tig0024802:-45012 Msa1420430:three_prime_UTR
ATGGTACAAATACAACGCCT+TGG 0.522364 tig0024802:+45042 None:intergenic
GAAAACCATGCCTGAAGGAA+AGG 0.522733 tig0024802:-45550 Msa1420430:CDS
GCTTCGTCGAAGAGCGGAAG+CGG 0.528323 tig0024802:-45956 Msa1420430:CDS
GAGGTACTAAAATAATAAAG+TGG 0.530105 tig0024802:-46088 Msa1420430:five_prime_UTR
AATTGTAATCAAATCGGAGC+TGG 0.532751 tig0024802:-46028 Msa1420430:five_prime_UTR
AAAACGTTGCGGATGAAGCT+GGG 0.534177 tig0024802:-45142 Msa1420430:CDS
GTAATCCTTTCCTTCAGGCA+TGG 0.536343 tig0024802:+45545 None:intergenic
CCTACAATATTATATAGTAA+AGG 0.541243 tig0024802:+44691 None:intergenic
TAACTTCGATCAACTTGTTG+AGG 0.544631 tig0024802:-45493 Msa1420430:intron
GAGTTCAAGACGAGTGGTGG+CGG 0.544654 tig0024802:+45829 None:intergenic
ACACAAGTCTTAATCAGAGC+AGG 0.545280 tig0024802:-44823 Msa1420430:three_prime_UTR
CGAGATTTAAAAGGTTAATG+TGG 0.550201 tig0024802:-44869 Msa1420430:three_prime_UTR
GGCGAGTTCAAGACGAGTGG+TGG 0.560196 tig0024802:+45826 None:intergenic
GTTAATGTGGAAATAAGCAT+TGG 0.560930 tig0024802:-44856 Msa1420430:three_prime_UTR
AGAGAGATCGTTGAGAGCGG+TGG 0.573255 tig0024802:+45802 None:intergenic
ACTGAGCCTAACATCAAATG+AGG 0.577162 tig0024802:+44655 None:intergenic
ATCAAATCGGAGCTGGAGGA+GGG 0.591866 tig0024802:-46021 Msa1420430:five_prime_UTR
TGTAATCAAATCGGAGCTGG+AGG 0.598285 tig0024802:-46025 Msa1420430:five_prime_UTR
GAGAAGCGGTGAGAGGAACA+AGG 0.602439 tig0024802:+45756 None:intergenic
AAAATGGCTTCGTCGAAGAG+CGG 0.608939 tig0024802:-45962 Msa1420430:CDS
ATATCGACGAGAACGTGATG+AGG 0.609043 tig0024802:+45707 None:intergenic
ACATAGAGAGAAGCGGTGAG+AGG 0.615765 tig0024802:+45749 None:intergenic
AAAATGGTGAGTAAAATTGG+TGG 0.618170 tig0024802:+44748 None:intergenic
AAACAGAAAACCATGCCTGA+AGG 0.621995 tig0024802:-45555 Msa1420430:intron
ACCATAGAAAATCATAACAC+TGG 0.623201 tig0024802:-45024 Msa1420430:three_prime_UTR
AGCAGGAACATAGAGAGAAG+CGG 0.640463 tig0024802:+45742 None:intergenic
GCGGAGCGATGACTCTGGAG+AGG 0.643180 tig0024802:-45937 Msa1420430:CDS
TAGTCCAAACTAATGTTCTG+AGG 0.643856 tig0024802:+44932 None:intergenic
TTAGGCTCAGTCGAAGATAG+GGG 0.656337 tig0024802:-44643 Msa1420430:three_prime_UTR
GAGAGAGAGATCGTTGAGAG+CGG 0.661545 tig0024802:+45799 None:intergenic
CGGAACTGGTTATTTCGTCG+AGG 0.673821 tig0024802:-45684 Msa1420430:intron
GGTGGCGAGTTCAAGACGAG+TGG 0.675476 tig0024802:+45823 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATAATTTATTTTGGTG+AGG - tig0024802:44641-44660 Msa1420430:three_prime_UTR 15.0%
!! TATTGTAATTGTAATCAAAT+CGG - tig0024802:44714-44733 Msa1420430:three_prime_UTR 15.0%
!! ACAGATCTAAATAAATTAAT+CGG + tig0024802:45107-45126 None:intergenic 15.0%
!!! TTAAACTTAATTGCAATTTT+CGG + tig0024802:44617-44636 None:intergenic 15.0%
!!! TAAAATAATTTATTTTGGTG+AGG - tig0024802:44641-44660 Msa1420430:three_prime_UTR 15.0%
!! TATTGTAATTGTAATCAAAT+CGG - tig0024802:44714-44733 Msa1420430:three_prime_UTR 15.0%
!! ACAGATCTAAATAAATTAAT+CGG + tig0024802:45107-45126 None:intergenic 15.0%
!! AGATCTAAATAAATTAATCG+GGG + tig0024802:45105-45124 None:intergenic 20.0%
!! CAGATCTAAATAAATTAATC+GGG + tig0024802:45106-45125 None:intergenic 20.0%
!! CTAGACAAAATATCAAATTA+GGG + tig0024802:45477-45496 None:intergenic 20.0%
!!! AATTCAATTACTTTTTTCCA+AGG - tig0024802:45689-45708 Msa1420430:CDS 20.0%
!! AAACGAATTCTTTCAATAAA+AGG + tig0024802:45783-45802 None:intergenic 20.0%
!! CCTACAATATTATATAGTAA+AGG + tig0024802:46060-46079 None:intergenic 20.0%
!! CCTTTACTATATAATATTGT+AGG - tig0024802:46057-46076 Msa1420430:five_prime_UTR 20.0%
!!! TAAACTTAATTGCAATTTTC+GGG + tig0024802:44616-44635 None:intergenic 20.0%
!! AGATCTAAATAAATTAATCG+GGG + tig0024802:45105-45124 None:intergenic 20.0%
!! CAGATCTAAATAAATTAATC+GGG + tig0024802:45106-45125 None:intergenic 20.0%
!! CTAGACAAAATATCAAATTA+GGG + tig0024802:45477-45496 None:intergenic 20.0%
!!! AATTCAATTACTTTTTTCCA+AGG - tig0024802:45689-45708 Msa1420430:CDS 20.0%
!! AAACGAATTCTTTCAATAAA+AGG + tig0024802:45783-45802 None:intergenic 20.0%
!! CCTACAATATTATATAGTAA+AGG + tig0024802:46060-46079 None:intergenic 20.0%
!! CCTTTACTATATAATATTGT+AGG - tig0024802:46057-46076 Msa1420430:five_prime_UTR 20.0%
!! GAGGTACTAAAATAATAAAG+TGG - tig0024802:44660-44679 Msa1420430:three_prime_UTR 25.0%
!! TATCGTATATGCTTCTTTTT+AGG - tig0024802:45126-45145 Msa1420430:CDS 25.0%
!! ATCGTATATGCTTCTTTTTA+GGG - tig0024802:45127-45146 Msa1420430:CDS 25.0%
!!! GTGTTAGTTTTATACTAATG+TGG - tig0024802:45400-45419 Msa1420430:intron 25.0%
! CCTAGACAAAATATCAAATT+AGG + tig0024802:45478-45497 None:intergenic 25.0%
!! CCTAATTTGATATTTTGTCT+AGG - tig0024802:45475-45494 Msa1420430:intron 25.0%
! AAAAATATCAGAGAGAAAAC+TGG + tig0024802:45512-45531 None:intergenic 25.0%
!! AGTTTTCTCTCTGATATTTT+TGG - tig0024802:45511-45530 Msa1420430:CDS 25.0%
!! TTTTAAATCTCGAATGTCAT+TGG + tig0024802:45871-45890 None:intergenic 25.0%
! AATGACATTCGAGATTTAAA+AGG - tig0024802:45870-45889 Msa1420430:CDS 25.0%
!! AAACTTAATTGCAATTTTCG+GGG + tig0024802:44615-44634 None:intergenic 25.0%
!! GAGGTACTAAAATAATAAAG+TGG - tig0024802:44660-44679 Msa1420430:three_prime_UTR 25.0%
!! TATCGTATATGCTTCTTTTT+AGG - tig0024802:45126-45145 Msa1420430:CDS 25.0%
!! ATCGTATATGCTTCTTTTTA+GGG - tig0024802:45127-45146 Msa1420430:CDS 25.0%
!!! GTGTTAGTTTTATACTAATG+TGG - tig0024802:45400-45419 Msa1420430:intron 25.0%
! CCTAGACAAAATATCAAATT+AGG + tig0024802:45478-45497 None:intergenic 25.0%
!! CCTAATTTGATATTTTGTCT+AGG - tig0024802:45475-45494 Msa1420430:intron 25.0%
! AAAAATATCAGAGAGAAAAC+TGG + tig0024802:45512-45531 None:intergenic 25.0%
!! AGTTTTCTCTCTGATATTTT+TGG - tig0024802:45511-45530 Msa1420430:CDS 25.0%
!! TTTTAAATCTCGAATGTCAT+TGG + tig0024802:45871-45890 None:intergenic 25.0%
! AATGACATTCGAGATTTAAA+AGG - tig0024802:45870-45889 Msa1420430:CDS 25.0%
AATCGAGAACCTTCAAAAAA+TGG - tig0024802:44770-44789 Msa1420430:three_prime_UTR 30.0%
!!! TATGCTTCTTTTTAGGGTTA+TGG - tig0024802:45133-45152 Msa1420430:CDS 30.0%
CTTGATGTAATTACAAATGC+AGG - tig0024802:45568-45587 Msa1420430:intron 30.0%
ATTCTATACCAGCTAACTTT+AGG + tig0024802:45672-45691 None:intergenic 30.0%
ACCATAGAAAATCATAACAC+TGG - tig0024802:45724-45743 Msa1420430:CDS 30.0%
! ACCAGTGTTATGATTTTCTA+TGG + tig0024802:45728-45747 None:intergenic 30.0%
! AGAACATTAGTTTGGACTAT+TGG - tig0024802:45820-45839 Msa1420430:CDS 30.0%
CGAGATTTAAAAGGTTAATG+TGG - tig0024802:45879-45898 Msa1420430:CDS 30.0%
GTTAATGTGGAAATAAGCAT+TGG - tig0024802:45892-45911 Msa1420430:CDS 30.0%
AAAATGGTGAGTAAAATTGG+TGG + tig0024802:46003-46022 None:intergenic 30.0%
GAGAAAATGGTGAGTAAAAT+TGG + tig0024802:46006-46025 None:intergenic 30.0%
AGTGTGTAAAAGAGAGAAAA+TGG + tig0024802:46019-46038 None:intergenic 30.0%
TCATTTCCTCATTTGATGTT+AGG - tig0024802:46087-46106 Msa1420430:five_prime_UTR 30.0%
AATCGAGAACCTTCAAAAAA+TGG - tig0024802:44770-44789 Msa1420430:three_prime_UTR 30.0%
!!! TATGCTTCTTTTTAGGGTTA+TGG - tig0024802:45133-45152 Msa1420430:CDS 30.0%
CTTGATGTAATTACAAATGC+AGG - tig0024802:45568-45587 Msa1420430:intron 30.0%
ATTCTATACCAGCTAACTTT+AGG + tig0024802:45672-45691 None:intergenic 30.0%
ACCATAGAAAATCATAACAC+TGG - tig0024802:45724-45743 Msa1420430:CDS 30.0%
! ACCAGTGTTATGATTTTCTA+TGG + tig0024802:45728-45747 None:intergenic 30.0%
! AGAACATTAGTTTGGACTAT+TGG - tig0024802:45820-45839 Msa1420430:CDS 30.0%
CGAGATTTAAAAGGTTAATG+TGG - tig0024802:45879-45898 Msa1420430:CDS 30.0%
GTTAATGTGGAAATAAGCAT+TGG - tig0024802:45892-45911 Msa1420430:CDS 30.0%
AAAATGGTGAGTAAAATTGG+TGG + tig0024802:46003-46022 None:intergenic 30.0%
GAGAAAATGGTGAGTAAAAT+TGG + tig0024802:46006-46025 None:intergenic 30.0%
AGTGTGTAAAAGAGAGAAAA+TGG + tig0024802:46019-46038 None:intergenic 30.0%
TCATTTCCTCATTTGATGTT+AGG - tig0024802:46087-46106 Msa1420430:five_prime_UTR 30.0%
AATTGTAATCAAATCGGAGC+TGG - tig0024802:44720-44739 Msa1420430:three_prime_UTR 35.0%
TGAACAGTTGAAAGCAGTTA+AGG - tig0024802:44842-44861 Msa1420430:three_prime_UTR 35.0%
AGTTAAGGAACAAGCAGATA+TGG - tig0024802:44857-44876 Msa1420430:three_prime_UTR 35.0%
CAAGACAGTCTCAACAATAT+CGG - tig0024802:44894-44913 Msa1420430:three_prime_UTR 35.0%
!! TAACTTCGATCAACTTGTTG+AGG - tig0024802:45255-45274 Msa1420430:intron 35.0%
CTAATGTGGTTATTCTGTTG+TGG - tig0024802:45414-45433 Msa1420430:intron 35.0%
ATCTTCATCCTAAAGTTAGC+TGG - tig0024802:45661-45680 Msa1420430:intron 35.0%
TAGTCCAAACTAATGTTCTG+AGG + tig0024802:45819-45838 None:intergenic 35.0%
!! AGCACTCCATGCTTTTTATT+AGG - tig0024802:45954-45973 Msa1420430:CDS 35.0%
!! ACTCCATGCTTTTTATTAGG+AGG - tig0024802:45957-45976 Msa1420430:CDS 35.0%
!! CTCCATGCTTTTTATTAGGA+GGG - tig0024802:45958-45977 Msa1420430:CDS 35.0%
ATCCCTCCTAATAAAAAGCA+TGG + tig0024802:45963-45982 None:intergenic 35.0%
! ACACTCAAAGTGCTCTTTAT+TGG - tig0024802:46034-46053 Msa1420430:five_prime_UTR 35.0%
AATTGTAATCAAATCGGAGC+TGG - tig0024802:44720-44739 Msa1420430:three_prime_UTR 35.0%
TGAACAGTTGAAAGCAGTTA+AGG - tig0024802:44842-44861 Msa1420430:three_prime_UTR 35.0%
AGTTAAGGAACAAGCAGATA+TGG - tig0024802:44857-44876 Msa1420430:three_prime_UTR 35.0%
CAAGACAGTCTCAACAATAT+CGG - tig0024802:44894-44913 Msa1420430:three_prime_UTR 35.0%
!! TAACTTCGATCAACTTGTTG+AGG - tig0024802:45255-45274 Msa1420430:intron 35.0%
CTAATGTGGTTATTCTGTTG+TGG - tig0024802:45414-45433 Msa1420430:intron 35.0%
ATCTTCATCCTAAAGTTAGC+TGG - tig0024802:45661-45680 Msa1420430:intron 35.0%
TAGTCCAAACTAATGTTCTG+AGG + tig0024802:45819-45838 None:intergenic 35.0%
!! AGCACTCCATGCTTTTTATT+AGG - tig0024802:45954-45973 Msa1420430:CDS 35.0%
!! ACTCCATGCTTTTTATTAGG+AGG - tig0024802:45957-45976 Msa1420430:CDS 35.0%
!! CTCCATGCTTTTTATTAGGA+GGG - tig0024802:45958-45977 Msa1420430:CDS 35.0%
ATCCCTCCTAATAAAAAGCA+TGG + tig0024802:45963-45982 None:intergenic 35.0%
! ACACTCAAAGTGCTCTTTAT+TGG - tig0024802:46034-46053 Msa1420430:five_prime_UTR 35.0%
!!! TCGACGAAGCCATTTTTTGA+AGG + tig0024802:44782-44801 None:intergenic 40.0%
TGTTGAGACTGTCTTGAAGA+AGG + tig0024802:44890-44909 None:intergenic 40.0%
AAACAGAAAACCATGCCTGA+AGG - tig0024802:45193-45212 Msa1420430:intron 40.0%
GTCCAAGAAGAAAAACGTTG+CGG - tig0024802:45595-45614 Msa1420430:intron 40.0%
! ATCCGCAACGTTTTTCTTCT+TGG + tig0024802:45600-45619 None:intergenic 40.0%
!!! TGAAGCTGGGCTTATTTTAC+AGG - tig0024802:45619-45638 Msa1420430:intron 40.0%
ATGGTACAAATACAACGCCT+TGG + tig0024802:45709-45728 None:intergenic 40.0%
! CATAACACTGGTGTTAGAGT+TGG - tig0024802:45736-45755 Msa1420430:CDS 40.0%
GATGCCTCAGAACATTAGTT+TGG - tig0024802:45812-45831 Msa1420430:CDS 40.0%
ACACAAGTCTTAATCAGAGC+AGG - tig0024802:45925-45944 Msa1420430:CDS 40.0%
ACTGAGCCTAACATCAAATG+AGG + tig0024802:46096-46115 None:intergenic 40.0%
GCGATTAAATATCAGGAGTC+AGG + tig0024802:46134-46153 None:intergenic 40.0%
!!! TCGACGAAGCCATTTTTTGA+AGG + tig0024802:44782-44801 None:intergenic 40.0%
TGTTGAGACTGTCTTGAAGA+AGG + tig0024802:44890-44909 None:intergenic 40.0%
AAACAGAAAACCATGCCTGA+AGG - tig0024802:45193-45212 Msa1420430:intron 40.0%
GTCCAAGAAGAAAAACGTTG+CGG - tig0024802:45595-45614 Msa1420430:intron 40.0%
! ATCCGCAACGTTTTTCTTCT+TGG + tig0024802:45600-45619 None:intergenic 40.0%
!!! TGAAGCTGGGCTTATTTTAC+AGG - tig0024802:45619-45638 Msa1420430:intron 40.0%
ATGGTACAAATACAACGCCT+TGG + tig0024802:45709-45728 None:intergenic 40.0%
! CATAACACTGGTGTTAGAGT+TGG - tig0024802:45736-45755 Msa1420430:CDS 40.0%
GATGCCTCAGAACATTAGTT+TGG - tig0024802:45812-45831 Msa1420430:CDS 40.0%
ACACAAGTCTTAATCAGAGC+AGG - tig0024802:45925-45944 Msa1420430:CDS 40.0%
ACTGAGCCTAACATCAAATG+AGG + tig0024802:46096-46115 None:intergenic 40.0%
GCGATTAAATATCAGGAGTC+AGG + tig0024802:46134-46153 None:intergenic 40.0%
TGTAATCAAATCGGAGCTGG+AGG - tig0024802:44723-44742 Msa1420430:three_prime_UTR 45.0%
AAAATGGCTTCGTCGAAGAG+CGG - tig0024802:44786-44805 Msa1420430:three_prime_UTR 45.0%
! GGATTTTACTGCCGAGAGAA+CGG + tig0024802:44974-44993 None:intergenic 45.0%
AGCAGGAACATAGAGAGAAG+CGG + tig0024802:45009-45028 None:intergenic 45.0%
TGAGGATCTTGAAGAGTAGC+AGG + tig0024802:45026-45045 None:intergenic 45.0%
ATATCGACGAGAACGTGATG+AGG + tig0024802:45044-45063 None:intergenic 45.0%
CATCACGTTCTCGTCGATAT+CGG - tig0024802:45044-45063 Msa1420430:three_prime_UTR 45.0%
GAAAACCATGCCTGAAGGAA+AGG - tig0024802:45198-45217 Msa1420430:intron 45.0%
GTAATCCTTTCCTTCAGGCA+TGG + tig0024802:45206-45225 None:intergenic 45.0%
TCGCAGTAATCCTTTCCTTC+AGG + tig0024802:45211-45230 None:intergenic 45.0%
AAAAACGTTGCGGATGAAGC+TGG - tig0024802:45605-45624 Msa1420430:intron 45.0%
AAAACGTTGCGGATGAAGCT+GGG - tig0024802:45606-45625 Msa1420430:intron 45.0%
AGGAGGGATCTTGTGATTGT+TGG - tig0024802:45974-45993 Msa1420430:exon 45.0%
GGAGGGATCTTGTGATTGTT+GGG - tig0024802:45975-45994 Msa1420430:exon 45.0%
TGTTAGGCTCAGTCGAAGAT+AGG - tig0024802:46103-46122 Msa1420430:five_prime_UTR 45.0%
GTTAGGCTCAGTCGAAGATA+GGG - tig0024802:46104-46123 Msa1420430:five_prime_UTR 45.0%
TTAGGCTCAGTCGAAGATAG+GGG - tig0024802:46105-46124 Msa1420430:five_prime_UTR 45.0%
TGTAATCAAATCGGAGCTGG+AGG - tig0024802:44723-44742 Msa1420430:three_prime_UTR 45.0%
AAAATGGCTTCGTCGAAGAG+CGG - tig0024802:44786-44805 Msa1420430:three_prime_UTR 45.0%
! GGATTTTACTGCCGAGAGAA+CGG + tig0024802:44974-44993 None:intergenic 45.0%
AGCAGGAACATAGAGAGAAG+CGG + tig0024802:45009-45028 None:intergenic 45.0%
TGAGGATCTTGAAGAGTAGC+AGG + tig0024802:45026-45045 None:intergenic 45.0%
ATATCGACGAGAACGTGATG+AGG + tig0024802:45044-45063 None:intergenic 45.0%
CATCACGTTCTCGTCGATAT+CGG - tig0024802:45044-45063 Msa1420430:three_prime_UTR 45.0%
GAAAACCATGCCTGAAGGAA+AGG - tig0024802:45198-45217 Msa1420430:intron 45.0%
GTAATCCTTTCCTTCAGGCA+TGG + tig0024802:45206-45225 None:intergenic 45.0%
TCGCAGTAATCCTTTCCTTC+AGG + tig0024802:45211-45230 None:intergenic 45.0%
AAAAACGTTGCGGATGAAGC+TGG - tig0024802:45605-45624 Msa1420430:intron 45.0%
AAAACGTTGCGGATGAAGCT+GGG - tig0024802:45606-45625 Msa1420430:intron 45.0%
AGGAGGGATCTTGTGATTGT+TGG - tig0024802:45974-45993 Msa1420430:exon 45.0%
GGAGGGATCTTGTGATTGTT+GGG - tig0024802:45975-45994 Msa1420430:exon 45.0%
TGTTAGGCTCAGTCGAAGAT+AGG - tig0024802:46103-46122 Msa1420430:five_prime_UTR 45.0%
GTTAGGCTCAGTCGAAGATA+GGG - tig0024802:46104-46123 Msa1420430:five_prime_UTR 45.0%
TTAGGCTCAGTCGAAGATAG+GGG - tig0024802:46105-46124 Msa1420430:five_prime_UTR 45.0%
!!! ATAAGTAAAATAATTTATTT+TGG - tig0024802:44636-44655 Msa1420430:three_prime_UTR 5.0%
!!! ATAAGTAAAATAATTTATTT+TGG - tig0024802:44636-44655 Msa1420430:three_prime_UTR 5.0%
AATCAAATCGGAGCTGGAGG+AGG - tig0024802:44726-44745 Msa1420430:three_prime_UTR 50.0%
ATCAAATCGGAGCTGGAGGA+GGG - tig0024802:44727-44746 Msa1420430:three_prime_UTR 50.0%
GAGAGAGAGATCGTTGAGAG+CGG + tig0024802:44952-44971 None:intergenic 50.0%
GATCTCTCTCTCCGTTCTCT+CGG - tig0024802:44960-44979 Msa1420430:three_prime_UTR 50.0%
ACATAGAGAGAAGCGGTGAG+AGG + tig0024802:45002-45021 None:intergenic 50.0%
GTTCTCGTCGATATCGGAAC+TGG - tig0024802:45050-45069 Msa1420430:three_prime_UTR 50.0%
! CGGAACTGGTTATTTCGTCG+AGG - tig0024802:45064-45083 Msa1420430:three_prime_UTR 50.0%
AATCAAATCGGAGCTGGAGG+AGG - tig0024802:44726-44745 Msa1420430:three_prime_UTR 50.0%
ATCAAATCGGAGCTGGAGGA+GGG - tig0024802:44727-44746 Msa1420430:three_prime_UTR 50.0%
GAGAGAGAGATCGTTGAGAG+CGG + tig0024802:44952-44971 None:intergenic 50.0%
GATCTCTCTCTCCGTTCTCT+CGG - tig0024802:44960-44979 Msa1420430:three_prime_UTR 50.0%
ACATAGAGAGAAGCGGTGAG+AGG + tig0024802:45002-45021 None:intergenic 50.0%
GTTCTCGTCGATATCGGAAC+TGG - tig0024802:45050-45069 Msa1420430:three_prime_UTR 50.0%
! CGGAACTGGTTATTTCGTCG+AGG - tig0024802:45064-45083 Msa1420430:three_prime_UTR 50.0%
GAGTTCAAGACGAGTGGTGG+CGG + tig0024802:44922-44941 None:intergenic 55.0%
AGAGAGATCGTTGAGAGCGG+TGG + tig0024802:44949-44968 None:intergenic 55.0%
GAGAAGCGGTGAGAGGAACA+AGG + tig0024802:44995-45014 None:intergenic 55.0%
GAGTTCAAGACGAGTGGTGG+CGG + tig0024802:44922-44941 None:intergenic 55.0%
AGAGAGATCGTTGAGAGCGG+TGG + tig0024802:44949-44968 None:intergenic 55.0%
GAGAAGCGGTGAGAGGAACA+AGG + tig0024802:44995-45014 None:intergenic 55.0%
TCGGAGCTGGAGGAGGGTTT+AGG - tig0024802:44733-44752 Msa1420430:three_prime_UTR 60.0%
CGGAGCTGGAGGAGGGTTTA+GGG - tig0024802:44734-44753 Msa1420430:three_prime_UTR 60.0%
GCTTCGTCGAAGAGCGGAAG+CGG - tig0024802:44792-44811 Msa1420430:three_prime_UTR 60.0%
GGCGAGTTCAAGACGAGTGG+TGG + tig0024802:44925-44944 None:intergenic 60.0%
GGTGGCGAGTTCAAGACGAG+TGG + tig0024802:44928-44947 None:intergenic 60.0%
GAGATCGTTGAGAGCGGTGG+TGG + tig0024802:44946-44965 None:intergenic 60.0%
TCGGAGCTGGAGGAGGGTTT+AGG - tig0024802:44733-44752 Msa1420430:three_prime_UTR 60.0%
CGGAGCTGGAGGAGGGTTTA+GGG - tig0024802:44734-44753 Msa1420430:three_prime_UTR 60.0%
GCTTCGTCGAAGAGCGGAAG+CGG - tig0024802:44792-44811 Msa1420430:three_prime_UTR 60.0%
GGCGAGTTCAAGACGAGTGG+TGG + tig0024802:44925-44944 None:intergenic 60.0%
GGTGGCGAGTTCAAGACGAG+TGG + tig0024802:44928-44947 None:intergenic 60.0%
GAGATCGTTGAGAGCGGTGG+TGG + tig0024802:44946-44965 None:intergenic 60.0%
CGGAAGCGGAGCGATGACTC+TGG - tig0024802:44806-44825 Msa1420430:three_prime_UTR 65.0%
! GCGGAGCGATGACTCTGGAG+AGG - tig0024802:44811-44830 Msa1420430:three_prime_UTR 65.0%
CGGAAGCGGAGCGATGACTC+TGG - tig0024802:44806-44825 Msa1420430:three_prime_UTR 65.0%
! GCGGAGCGATGACTCTGGAG+AGG - tig0024802:44811-44830 Msa1420430:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
tig0024802 gene 44615 46155 44615 ID=Msa1420430;Name=Msa1420430
tig0024802 mRNA 44615 46155 44615 ID=Msa1420430-mRNA-1;Parent=Msa1420430;Name=Msa1420430-mRNA-1;_AED=0.01;_eAED=0.01;_QI=174|1|1|1|1|1|3|482|152
tig0024802 exon 44615 45180 44615 ID=Msa1420430-mRNA-1:exon:1466;Parent=Msa1420430-mRNA-1
tig0024802 exon 45494 45571 45494 ID=Msa1420430-mRNA-1:exon:1465;Parent=Msa1420430-mRNA-1
tig0024802 exon 45685 46155 45685 ID=Msa1420430-mRNA-1:exon:1464;Parent=Msa1420430-mRNA-1
tig0024802 five_prime_UTR 45982 46155 45982 ID=Msa1420430-mRNA-1:five_prime_utr;Parent=Msa1420430-mRNA-1
tig0024802 CDS 45685 45981 45685 ID=Msa1420430-mRNA-1:cds;Parent=Msa1420430-mRNA-1
tig0024802 CDS 45494 45571 45494 ID=Msa1420430-mRNA-1:cds;Parent=Msa1420430-mRNA-1
tig0024802 CDS 45097 45180 45097 ID=Msa1420430-mRNA-1:cds;Parent=Msa1420430-mRNA-1
tig0024802 three_prime_UTR 44615 45096 44615 ID=Msa1420430-mRNA-1:three_prime_utr;Parent=Msa1420430-mRNA-1
Gene Sequence

>Msa1420430

ATGGCTTCGTCGAAGAGCGGAAGCGGAGCGATGACTCTGGAGAGGATGAGCGTTGAACAGTTGAAAGCAGTTAAGGAACAAGCAGATATGGAAGTTAACCTTCTTCAAGACAGTCTCAACAATATCGGCACCGCCACCACTCGTCTTGAACTCGCCACCACCGCTCTCAACGATCTCTCTCTCCGTTCTCTCGGCAGTAAAATCCTTGTTCCTCTCACCGCTTCTCTCTATGTTCCTGCTACTCTTCAAGATCCTCATCACGTTCTCGTCGATATCGGAACTGGTTATTTCGTCGAGAAAACCATGCCTGAAGGAAAGGATTACTGCGAGCGCAAAATCAACTTACTCAAATCTAACTTCGATCAACTTGTTGAGGTTGCGTCCAAGAAGAAAAACGTTGCGGATGAAGCTGGGCTTATTTTACAGGCAAAATTGAAGCAATTAGCATCTTCATCCTAA
Protein sequence

>Msa1420430

MASSKSGSGAMTLERMSVEQLKAVKEQADMEVNLLQDSLNNIGTATTRLELATTALNDLSLRSLGSKILVPLTASLYVPATLQDPHHVLVDIGTGYFVEKTMPEGKDYCERKINLLKSNFDQLVEVASKKKNVADEAGLILQAKLKQLASSS*