AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1445370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1445370 MtrunA17_Chr1g0211971 99.342 152 1 0 1 152 1 152 6.42e-109 306
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1445370 AT5G23290.1 72.368 152 39 2 1 152 1 149 2.91e-73 216

Find 66 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTCAAAGTGCTCTTTAT+TGG 0.161123 tig0031045:-33221 Msa1445370:three_prime_UTR
AAACGAATTCTTTCAATAAA+AGG 0.178416 tig0031045:+33475 None:intergenic
ATAAGTAAAATAATTTATTT+TGG 0.191605 tig0031045:-34619 Msa1445370:five_prime_UTR
AATGACATTCGAGATTTAAA+AGG 0.255512 tig0031045:-33385 Msa1445370:three_prime_UTR
TCGGAGCTGGAGGAGGGTTT+AGG 0.263067 tig0031045:-34522 Msa1445370:five_prime_UTR
AGAACATTAGTTTGGACTAT+TGG 0.267818 tig0031045:-33435 Msa1445370:three_prime_UTR
ATTCTATACCAGCTAACTTT+AGG 0.287756 tig0031045:+33586 None:intergenic
CCTTTACTATATAATATTGT+AGG 0.292567 tig0031045:-33198 Msa1445370:three_prime_UTR
GAGAAAATGGTGAGTAAAAT+TGG 0.295052 tig0031045:+33252 None:intergenic
TCATTTCCTCATTTGATGTT+AGG 0.333820 tig0031045:-33168 Msa1445370:three_prime_UTR
CATCACGTTCTCGTCGATAT+CGG 0.346200 tig0031045:-34211 Msa1445370:CDS
CGGAGCTGGAGGAGGGTTTA+GGG 0.346867 tig0031045:-34521 Msa1445370:five_prime_UTR
GATGCCTCAGAACATTAGTT+TGG 0.367543 tig0031045:-33443 Msa1445370:three_prime_UTR
TGTTAGGCTCAGTCGAAGAT+AGG 0.389749 tig0031045:-33152 Msa1445370:three_prime_UTR
AGTTAAGGAACAAGCAGATA+TGG 0.392343 tig0031045:-34398 Msa1445370:CDS
GAGAGGGGCGATTAAATATC+AGG 0.394744 tig0031045:+33117 None:intergenic
TCGCAGTAATCCTTTCCTTC+AGG 0.397139 tig0031045:+34047 None:intergenic
GGAGGGATCTTGTGATTGTT+GGG 0.397497 tig0031045:-33280 Msa1445370:three_prime_UTR
AGGAGGGATCTTGTGATTGT+TGG 0.405237 tig0031045:-33281 Msa1445370:three_prime_UTR
TATTGTAATTGTAATCAAAT+CGG 0.414828 tig0031045:-34541 Msa1445370:five_prime_UTR
TGAACAGTTGAAAGCAGTTA+AGG 0.432363 tig0031045:-34413 Msa1445370:CDS
AGTGTGTAAAAGAGAGAAAA+TGG 0.438240 tig0031045:+33239 None:intergenic
GATCTCTCTCTCCGTTCTCT+CGG 0.450391 tig0031045:-34295 Msa1445370:CDS
CGGAAGCGGAGCGATGACTC+TGG 0.463988 tig0031045:-34449 Msa1445370:CDS
CAAGACAGTCTCAACAATAT+CGG 0.466377 tig0031045:-34361 Msa1445370:CDS
GCGATTAAATATCAGGAGTC+AGG 0.479053 tig0031045:+33124 None:intergenic
TGTTGAGACTGTCTTGAAGA+AGG 0.480129 tig0031045:+34368 None:intergenic
GTTAGGCTCAGTCGAAGATA+GGG 0.485542 tig0031045:-33151 Msa1445370:three_prime_UTR
AATCAAATCGGAGCTGGAGG+AGG 0.507340 tig0031045:-34529 Msa1445370:five_prime_UTR
ATCTTCATCCTAAAGTTAGC+TGG 0.507912 tig0031045:-33594 Msa1445370:exon
GTTCTCGTCGATATCGGAAC+TGG 0.508671 tig0031045:-34205 Msa1445370:CDS
TGAGGATCTTGAAGAGTAGC+AGG 0.512402 tig0031045:+34232 None:intergenic
GAGATCGTTGAGAGCGGTGG+TGG 0.515505 tig0031045:+34312 None:intergenic
CATAACACTGGTGTTAGAGT+TGG 0.520800 tig0031045:-33519 Msa1445370:three_prime_UTR
ATGGTACAAATACAACGCCT+TGG 0.522364 tig0031045:+33549 None:intergenic
GAAAACCATGCCTGAAGGAA+AGG 0.522733 tig0031045:-34057 Msa1445370:CDS
GCTTCGTCGAAGAGCGGAAG+CGG 0.528323 tig0031045:-34463 Msa1445370:CDS
GAGGTACTAAAATAATAAAG+TGG 0.530105 tig0031045:-34595 Msa1445370:five_prime_UTR
AATTGTAATCAAATCGGAGC+TGG 0.532751 tig0031045:-34535 Msa1445370:five_prime_UTR
AAAACGTTGCGGATGAAGCT+GGG 0.534177 tig0031045:-33649 Msa1445370:CDS
GTAATCCTTTCCTTCAGGCA+TGG 0.536343 tig0031045:+34052 None:intergenic
CCTACAATATTATATAGTAA+AGG 0.541243 tig0031045:+33198 None:intergenic
TAACTTCGATCAACTTGTTG+AGG 0.544631 tig0031045:-34000 Msa1445370:intron
GAGTTCAAGACGAGTGGTGG+CGG 0.544654 tig0031045:+34336 None:intergenic
ACACAAGTCTTAATCAGAGC+AGG 0.545280 tig0031045:-33330 Msa1445370:three_prime_UTR
CGAGATTTAAAAGGTTAATG+TGG 0.550201 tig0031045:-33376 Msa1445370:three_prime_UTR
GGCGAGTTCAAGACGAGTGG+TGG 0.560196 tig0031045:+34333 None:intergenic
GTTAATGTGGAAATAAGCAT+TGG 0.560930 tig0031045:-33363 Msa1445370:three_prime_UTR
AGAGAGATCGTTGAGAGCGG+TGG 0.573255 tig0031045:+34309 None:intergenic
ACTGAGCCTAACATCAAATG+AGG 0.577162 tig0031045:+33162 None:intergenic
ATCAAATCGGAGCTGGAGGA+GGG 0.591866 tig0031045:-34528 Msa1445370:five_prime_UTR
TGTAATCAAATCGGAGCTGG+AGG 0.598285 tig0031045:-34532 Msa1445370:five_prime_UTR
GAGAAGCGGTGAGAGGAACA+AGG 0.602439 tig0031045:+34263 None:intergenic
AAAATGGCTTCGTCGAAGAG+CGG 0.608939 tig0031045:-34469 Msa1445370:CDS
ATATCGACGAGAACGTGATG+AGG 0.609043 tig0031045:+34214 None:intergenic
ACATAGAGAGAAGCGGTGAG+AGG 0.615765 tig0031045:+34256 None:intergenic
AAAATGGTGAGTAAAATTGG+TGG 0.618170 tig0031045:+33255 None:intergenic
AAACAGAAAACCATGCCTGA+AGG 0.621995 tig0031045:-34062 Msa1445370:intron
ACCATAGAAAATCATAACAC+TGG 0.623201 tig0031045:-33531 Msa1445370:three_prime_UTR
AGCAGGAACATAGAGAGAAG+CGG 0.640463 tig0031045:+34249 None:intergenic
GCGGAGCGATGACTCTGGAG+AGG 0.643180 tig0031045:-34444 Msa1445370:CDS
TAGTCCAAACTAATGTTCTG+AGG 0.643856 tig0031045:+33439 None:intergenic
TTAGGCTCAGTCGAAGATAG+GGG 0.656337 tig0031045:-33150 Msa1445370:three_prime_UTR
GAGAGAGAGATCGTTGAGAG+CGG 0.661545 tig0031045:+34306 None:intergenic
CGGAACTGGTTATTTCGTCG+AGG 0.673821 tig0031045:-34191 Msa1445370:intron
GGTGGCGAGTTCAAGACGAG+TGG 0.675476 tig0031045:+34330 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATAATTTATTTTGGTG+AGG - tig0031045:33148-33167 Msa1445370:three_prime_UTR 15.0%
!! TATTGTAATTGTAATCAAAT+CGG - tig0031045:33221-33240 Msa1445370:three_prime_UTR 15.0%
!! ACAGATCTAAATAAATTAAT+CGG + tig0031045:33614-33633 None:intergenic 15.0%
!!! TTAAACTTAATTGCAATTTT+CGG + tig0031045:33124-33143 None:intergenic 15.0%
!!! TAAAATAATTTATTTTGGTG+AGG - tig0031045:33148-33167 Msa1445370:three_prime_UTR 15.0%
!! TATTGTAATTGTAATCAAAT+CGG - tig0031045:33221-33240 Msa1445370:three_prime_UTR 15.0%
!! ACAGATCTAAATAAATTAAT+CGG + tig0031045:33614-33633 None:intergenic 15.0%
!! AGATCTAAATAAATTAATCG+GGG + tig0031045:33612-33631 None:intergenic 20.0%
!! CAGATCTAAATAAATTAATC+GGG + tig0031045:33613-33632 None:intergenic 20.0%
!! CTAGACAAAATATCAAATTA+GGG + tig0031045:33984-34003 None:intergenic 20.0%
!!! AATTCAATTACTTTTTTCCA+AGG - tig0031045:34196-34215 Msa1445370:CDS 20.0%
!! AAACGAATTCTTTCAATAAA+AGG + tig0031045:34290-34309 None:intergenic 20.0%
!! CCTACAATATTATATAGTAA+AGG + tig0031045:34567-34586 None:intergenic 20.0%
!! CCTTTACTATATAATATTGT+AGG - tig0031045:34564-34583 Msa1445370:five_prime_UTR 20.0%
!!! TAAACTTAATTGCAATTTTC+GGG + tig0031045:33123-33142 None:intergenic 20.0%
!! AGATCTAAATAAATTAATCG+GGG + tig0031045:33612-33631 None:intergenic 20.0%
!! CAGATCTAAATAAATTAATC+GGG + tig0031045:33613-33632 None:intergenic 20.0%
!! CTAGACAAAATATCAAATTA+GGG + tig0031045:33984-34003 None:intergenic 20.0%
!!! AATTCAATTACTTTTTTCCA+AGG - tig0031045:34196-34215 Msa1445370:CDS 20.0%
!! AAACGAATTCTTTCAATAAA+AGG + tig0031045:34290-34309 None:intergenic 20.0%
!! CCTACAATATTATATAGTAA+AGG + tig0031045:34567-34586 None:intergenic 20.0%
!! CCTTTACTATATAATATTGT+AGG - tig0031045:34564-34583 Msa1445370:five_prime_UTR 20.0%
!! GAGGTACTAAAATAATAAAG+TGG - tig0031045:33167-33186 Msa1445370:three_prime_UTR 25.0%
!! TATCGTATATGCTTCTTTTT+AGG - tig0031045:33633-33652 Msa1445370:CDS 25.0%
!! ATCGTATATGCTTCTTTTTA+GGG - tig0031045:33634-33653 Msa1445370:CDS 25.0%
!!! GTGTTAGTTTTATACTAATG+TGG - tig0031045:33907-33926 Msa1445370:intron 25.0%
! CCTAGACAAAATATCAAATT+AGG + tig0031045:33985-34004 None:intergenic 25.0%
!! CCTAATTTGATATTTTGTCT+AGG - tig0031045:33982-34001 Msa1445370:intron 25.0%
! AAAAATATCAGAGAGAAAAC+TGG + tig0031045:34019-34038 None:intergenic 25.0%
!! AGTTTTCTCTCTGATATTTT+TGG - tig0031045:34018-34037 Msa1445370:CDS 25.0%
!! TTTTAAATCTCGAATGTCAT+TGG + tig0031045:34378-34397 None:intergenic 25.0%
! AATGACATTCGAGATTTAAA+AGG - tig0031045:34377-34396 Msa1445370:CDS 25.0%
!! AAACTTAATTGCAATTTTCG+GGG + tig0031045:33122-33141 None:intergenic 25.0%
!! GAGGTACTAAAATAATAAAG+TGG - tig0031045:33167-33186 Msa1445370:three_prime_UTR 25.0%
!! TATCGTATATGCTTCTTTTT+AGG - tig0031045:33633-33652 Msa1445370:CDS 25.0%
!! ATCGTATATGCTTCTTTTTA+GGG - tig0031045:33634-33653 Msa1445370:CDS 25.0%
!!! GTGTTAGTTTTATACTAATG+TGG - tig0031045:33907-33926 Msa1445370:intron 25.0%
! CCTAGACAAAATATCAAATT+AGG + tig0031045:33985-34004 None:intergenic 25.0%
!! CCTAATTTGATATTTTGTCT+AGG - tig0031045:33982-34001 Msa1445370:intron 25.0%
! AAAAATATCAGAGAGAAAAC+TGG + tig0031045:34019-34038 None:intergenic 25.0%
!! AGTTTTCTCTCTGATATTTT+TGG - tig0031045:34018-34037 Msa1445370:CDS 25.0%
!! TTTTAAATCTCGAATGTCAT+TGG + tig0031045:34378-34397 None:intergenic 25.0%
! AATGACATTCGAGATTTAAA+AGG - tig0031045:34377-34396 Msa1445370:CDS 25.0%
AATCGAGAACCTTCAAAAAA+TGG - tig0031045:33277-33296 Msa1445370:three_prime_UTR 30.0%
!!! TATGCTTCTTTTTAGGGTTA+TGG - tig0031045:33640-33659 Msa1445370:CDS 30.0%
CTTGATGTAATTACAAATGC+AGG - tig0031045:34075-34094 Msa1445370:intron 30.0%
ATTCTATACCAGCTAACTTT+AGG + tig0031045:34179-34198 None:intergenic 30.0%
ACCATAGAAAATCATAACAC+TGG - tig0031045:34231-34250 Msa1445370:CDS 30.0%
! ACCAGTGTTATGATTTTCTA+TGG + tig0031045:34235-34254 None:intergenic 30.0%
! AGAACATTAGTTTGGACTAT+TGG - tig0031045:34327-34346 Msa1445370:CDS 30.0%
CGAGATTTAAAAGGTTAATG+TGG - tig0031045:34386-34405 Msa1445370:CDS 30.0%
GTTAATGTGGAAATAAGCAT+TGG - tig0031045:34399-34418 Msa1445370:CDS 30.0%
AAAATGGTGAGTAAAATTGG+TGG + tig0031045:34510-34529 None:intergenic 30.0%
GAGAAAATGGTGAGTAAAAT+TGG + tig0031045:34513-34532 None:intergenic 30.0%
AGTGTGTAAAAGAGAGAAAA+TGG + tig0031045:34526-34545 None:intergenic 30.0%
TCATTTCCTCATTTGATGTT+AGG - tig0031045:34594-34613 Msa1445370:five_prime_UTR 30.0%
AATCGAGAACCTTCAAAAAA+TGG - tig0031045:33277-33296 Msa1445370:three_prime_UTR 30.0%
!!! TATGCTTCTTTTTAGGGTTA+TGG - tig0031045:33640-33659 Msa1445370:CDS 30.0%
CTTGATGTAATTACAAATGC+AGG - tig0031045:34075-34094 Msa1445370:intron 30.0%
ATTCTATACCAGCTAACTTT+AGG + tig0031045:34179-34198 None:intergenic 30.0%
ACCATAGAAAATCATAACAC+TGG - tig0031045:34231-34250 Msa1445370:CDS 30.0%
! ACCAGTGTTATGATTTTCTA+TGG + tig0031045:34235-34254 None:intergenic 30.0%
! AGAACATTAGTTTGGACTAT+TGG - tig0031045:34327-34346 Msa1445370:CDS 30.0%
CGAGATTTAAAAGGTTAATG+TGG - tig0031045:34386-34405 Msa1445370:CDS 30.0%
GTTAATGTGGAAATAAGCAT+TGG - tig0031045:34399-34418 Msa1445370:CDS 30.0%
AAAATGGTGAGTAAAATTGG+TGG + tig0031045:34510-34529 None:intergenic 30.0%
GAGAAAATGGTGAGTAAAAT+TGG + tig0031045:34513-34532 None:intergenic 30.0%
AGTGTGTAAAAGAGAGAAAA+TGG + tig0031045:34526-34545 None:intergenic 30.0%
TCATTTCCTCATTTGATGTT+AGG - tig0031045:34594-34613 Msa1445370:five_prime_UTR 30.0%
AATTGTAATCAAATCGGAGC+TGG - tig0031045:33227-33246 Msa1445370:three_prime_UTR 35.0%
TGAACAGTTGAAAGCAGTTA+AGG - tig0031045:33349-33368 Msa1445370:three_prime_UTR 35.0%
AGTTAAGGAACAAGCAGATA+TGG - tig0031045:33364-33383 Msa1445370:three_prime_UTR 35.0%
CAAGACAGTCTCAACAATAT+CGG - tig0031045:33401-33420 Msa1445370:three_prime_UTR 35.0%
!! TAACTTCGATCAACTTGTTG+AGG - tig0031045:33762-33781 Msa1445370:intron 35.0%
CTAATGTGGTTATTCTGTTG+TGG - tig0031045:33921-33940 Msa1445370:intron 35.0%
ATCTTCATCCTAAAGTTAGC+TGG - tig0031045:34168-34187 Msa1445370:intron 35.0%
TAGTCCAAACTAATGTTCTG+AGG + tig0031045:34326-34345 None:intergenic 35.0%
!! AGCACTCCATGCTTTTTATT+AGG - tig0031045:34461-34480 Msa1445370:CDS 35.0%
!! ACTCCATGCTTTTTATTAGG+AGG - tig0031045:34464-34483 Msa1445370:CDS 35.0%
!! CTCCATGCTTTTTATTAGGA+GGG - tig0031045:34465-34484 Msa1445370:CDS 35.0%
ATCCCTCCTAATAAAAAGCA+TGG + tig0031045:34470-34489 None:intergenic 35.0%
! ACACTCAAAGTGCTCTTTAT+TGG - tig0031045:34541-34560 Msa1445370:five_prime_UTR 35.0%
AATTGTAATCAAATCGGAGC+TGG - tig0031045:33227-33246 Msa1445370:three_prime_UTR 35.0%
TGAACAGTTGAAAGCAGTTA+AGG - tig0031045:33349-33368 Msa1445370:three_prime_UTR 35.0%
AGTTAAGGAACAAGCAGATA+TGG - tig0031045:33364-33383 Msa1445370:three_prime_UTR 35.0%
CAAGACAGTCTCAACAATAT+CGG - tig0031045:33401-33420 Msa1445370:three_prime_UTR 35.0%
!! TAACTTCGATCAACTTGTTG+AGG - tig0031045:33762-33781 Msa1445370:intron 35.0%
CTAATGTGGTTATTCTGTTG+TGG - tig0031045:33921-33940 Msa1445370:intron 35.0%
ATCTTCATCCTAAAGTTAGC+TGG - tig0031045:34168-34187 Msa1445370:intron 35.0%
TAGTCCAAACTAATGTTCTG+AGG + tig0031045:34326-34345 None:intergenic 35.0%
!! AGCACTCCATGCTTTTTATT+AGG - tig0031045:34461-34480 Msa1445370:CDS 35.0%
!! ACTCCATGCTTTTTATTAGG+AGG - tig0031045:34464-34483 Msa1445370:CDS 35.0%
!! CTCCATGCTTTTTATTAGGA+GGG - tig0031045:34465-34484 Msa1445370:CDS 35.0%
ATCCCTCCTAATAAAAAGCA+TGG + tig0031045:34470-34489 None:intergenic 35.0%
! ACACTCAAAGTGCTCTTTAT+TGG - tig0031045:34541-34560 Msa1445370:five_prime_UTR 35.0%
!!! TCGACGAAGCCATTTTTTGA+AGG + tig0031045:33289-33308 None:intergenic 40.0%
TGTTGAGACTGTCTTGAAGA+AGG + tig0031045:33397-33416 None:intergenic 40.0%
AAACAGAAAACCATGCCTGA+AGG - tig0031045:33700-33719 Msa1445370:intron 40.0%
GTCCAAGAAGAAAAACGTTG+CGG - tig0031045:34102-34121 Msa1445370:intron 40.0%
! ATCCGCAACGTTTTTCTTCT+TGG + tig0031045:34107-34126 None:intergenic 40.0%
!!! TGAAGCTGGGCTTATTTTAC+AGG - tig0031045:34126-34145 Msa1445370:intron 40.0%
ATGGTACAAATACAACGCCT+TGG + tig0031045:34216-34235 None:intergenic 40.0%
! CATAACACTGGTGTTAGAGT+TGG - tig0031045:34243-34262 Msa1445370:CDS 40.0%
GATGCCTCAGAACATTAGTT+TGG - tig0031045:34319-34338 Msa1445370:CDS 40.0%
ACACAAGTCTTAATCAGAGC+AGG - tig0031045:34432-34451 Msa1445370:CDS 40.0%
ACTGAGCCTAACATCAAATG+AGG + tig0031045:34603-34622 None:intergenic 40.0%
GCGATTAAATATCAGGAGTC+AGG + tig0031045:34641-34660 None:intergenic 40.0%
!!! TCGACGAAGCCATTTTTTGA+AGG + tig0031045:33289-33308 None:intergenic 40.0%
TGTTGAGACTGTCTTGAAGA+AGG + tig0031045:33397-33416 None:intergenic 40.0%
AAACAGAAAACCATGCCTGA+AGG - tig0031045:33700-33719 Msa1445370:intron 40.0%
GTCCAAGAAGAAAAACGTTG+CGG - tig0031045:34102-34121 Msa1445370:intron 40.0%
! ATCCGCAACGTTTTTCTTCT+TGG + tig0031045:34107-34126 None:intergenic 40.0%
!!! TGAAGCTGGGCTTATTTTAC+AGG - tig0031045:34126-34145 Msa1445370:intron 40.0%
ATGGTACAAATACAACGCCT+TGG + tig0031045:34216-34235 None:intergenic 40.0%
! CATAACACTGGTGTTAGAGT+TGG - tig0031045:34243-34262 Msa1445370:CDS 40.0%
GATGCCTCAGAACATTAGTT+TGG - tig0031045:34319-34338 Msa1445370:CDS 40.0%
ACACAAGTCTTAATCAGAGC+AGG - tig0031045:34432-34451 Msa1445370:CDS 40.0%
ACTGAGCCTAACATCAAATG+AGG + tig0031045:34603-34622 None:intergenic 40.0%
GCGATTAAATATCAGGAGTC+AGG + tig0031045:34641-34660 None:intergenic 40.0%
TGTAATCAAATCGGAGCTGG+AGG - tig0031045:33230-33249 Msa1445370:three_prime_UTR 45.0%
AAAATGGCTTCGTCGAAGAG+CGG - tig0031045:33293-33312 Msa1445370:three_prime_UTR 45.0%
! GGATTTTACTGCCGAGAGAA+CGG + tig0031045:33481-33500 None:intergenic 45.0%
AGCAGGAACATAGAGAGAAG+CGG + tig0031045:33516-33535 None:intergenic 45.0%
TGAGGATCTTGAAGAGTAGC+AGG + tig0031045:33533-33552 None:intergenic 45.0%
ATATCGACGAGAACGTGATG+AGG + tig0031045:33551-33570 None:intergenic 45.0%
CATCACGTTCTCGTCGATAT+CGG - tig0031045:33551-33570 Msa1445370:three_prime_UTR 45.0%
GAAAACCATGCCTGAAGGAA+AGG - tig0031045:33705-33724 Msa1445370:intron 45.0%
GTAATCCTTTCCTTCAGGCA+TGG + tig0031045:33713-33732 None:intergenic 45.0%
TCGCAGTAATCCTTTCCTTC+AGG + tig0031045:33718-33737 None:intergenic 45.0%
AAAAACGTTGCGGATGAAGC+TGG - tig0031045:34112-34131 Msa1445370:intron 45.0%
AAAACGTTGCGGATGAAGCT+GGG - tig0031045:34113-34132 Msa1445370:intron 45.0%
AGGAGGGATCTTGTGATTGT+TGG - tig0031045:34481-34500 Msa1445370:exon 45.0%
GGAGGGATCTTGTGATTGTT+GGG - tig0031045:34482-34501 Msa1445370:exon 45.0%
TGTTAGGCTCAGTCGAAGAT+AGG - tig0031045:34610-34629 Msa1445370:five_prime_UTR 45.0%
GTTAGGCTCAGTCGAAGATA+GGG - tig0031045:34611-34630 Msa1445370:five_prime_UTR 45.0%
TTAGGCTCAGTCGAAGATAG+GGG - tig0031045:34612-34631 Msa1445370:five_prime_UTR 45.0%
TGTAATCAAATCGGAGCTGG+AGG - tig0031045:33230-33249 Msa1445370:three_prime_UTR 45.0%
AAAATGGCTTCGTCGAAGAG+CGG - tig0031045:33293-33312 Msa1445370:three_prime_UTR 45.0%
! GGATTTTACTGCCGAGAGAA+CGG + tig0031045:33481-33500 None:intergenic 45.0%
AGCAGGAACATAGAGAGAAG+CGG + tig0031045:33516-33535 None:intergenic 45.0%
TGAGGATCTTGAAGAGTAGC+AGG + tig0031045:33533-33552 None:intergenic 45.0%
ATATCGACGAGAACGTGATG+AGG + tig0031045:33551-33570 None:intergenic 45.0%
CATCACGTTCTCGTCGATAT+CGG - tig0031045:33551-33570 Msa1445370:three_prime_UTR 45.0%
GAAAACCATGCCTGAAGGAA+AGG - tig0031045:33705-33724 Msa1445370:intron 45.0%
GTAATCCTTTCCTTCAGGCA+TGG + tig0031045:33713-33732 None:intergenic 45.0%
TCGCAGTAATCCTTTCCTTC+AGG + tig0031045:33718-33737 None:intergenic 45.0%
AAAAACGTTGCGGATGAAGC+TGG - tig0031045:34112-34131 Msa1445370:intron 45.0%
AAAACGTTGCGGATGAAGCT+GGG - tig0031045:34113-34132 Msa1445370:intron 45.0%
AGGAGGGATCTTGTGATTGT+TGG - tig0031045:34481-34500 Msa1445370:exon 45.0%
GGAGGGATCTTGTGATTGTT+GGG - tig0031045:34482-34501 Msa1445370:exon 45.0%
TGTTAGGCTCAGTCGAAGAT+AGG - tig0031045:34610-34629 Msa1445370:five_prime_UTR 45.0%
GTTAGGCTCAGTCGAAGATA+GGG - tig0031045:34611-34630 Msa1445370:five_prime_UTR 45.0%
TTAGGCTCAGTCGAAGATAG+GGG - tig0031045:34612-34631 Msa1445370:five_prime_UTR 45.0%
!!! ATAAGTAAAATAATTTATTT+TGG - tig0031045:33143-33162 Msa1445370:three_prime_UTR 5.0%
!!! ATAAGTAAAATAATTTATTT+TGG - tig0031045:33143-33162 Msa1445370:three_prime_UTR 5.0%
AATCAAATCGGAGCTGGAGG+AGG - tig0031045:33233-33252 Msa1445370:three_prime_UTR 50.0%
ATCAAATCGGAGCTGGAGGA+GGG - tig0031045:33234-33253 Msa1445370:three_prime_UTR 50.0%
GAGAGAGAGATCGTTGAGAG+CGG + tig0031045:33459-33478 None:intergenic 50.0%
GATCTCTCTCTCCGTTCTCT+CGG - tig0031045:33467-33486 Msa1445370:three_prime_UTR 50.0%
ACATAGAGAGAAGCGGTGAG+AGG + tig0031045:33509-33528 None:intergenic 50.0%
GTTCTCGTCGATATCGGAAC+TGG - tig0031045:33557-33576 Msa1445370:three_prime_UTR 50.0%
! CGGAACTGGTTATTTCGTCG+AGG - tig0031045:33571-33590 Msa1445370:three_prime_UTR 50.0%
AATCAAATCGGAGCTGGAGG+AGG - tig0031045:33233-33252 Msa1445370:three_prime_UTR 50.0%
ATCAAATCGGAGCTGGAGGA+GGG - tig0031045:33234-33253 Msa1445370:three_prime_UTR 50.0%
GAGAGAGAGATCGTTGAGAG+CGG + tig0031045:33459-33478 None:intergenic 50.0%
GATCTCTCTCTCCGTTCTCT+CGG - tig0031045:33467-33486 Msa1445370:three_prime_UTR 50.0%
ACATAGAGAGAAGCGGTGAG+AGG + tig0031045:33509-33528 None:intergenic 50.0%
GTTCTCGTCGATATCGGAAC+TGG - tig0031045:33557-33576 Msa1445370:three_prime_UTR 50.0%
! CGGAACTGGTTATTTCGTCG+AGG - tig0031045:33571-33590 Msa1445370:three_prime_UTR 50.0%
GAGTTCAAGACGAGTGGTGG+CGG + tig0031045:33429-33448 None:intergenic 55.0%
AGAGAGATCGTTGAGAGCGG+TGG + tig0031045:33456-33475 None:intergenic 55.0%
GAGAAGCGGTGAGAGGAACA+AGG + tig0031045:33502-33521 None:intergenic 55.0%
GAGTTCAAGACGAGTGGTGG+CGG + tig0031045:33429-33448 None:intergenic 55.0%
AGAGAGATCGTTGAGAGCGG+TGG + tig0031045:33456-33475 None:intergenic 55.0%
GAGAAGCGGTGAGAGGAACA+AGG + tig0031045:33502-33521 None:intergenic 55.0%
TCGGAGCTGGAGGAGGGTTT+AGG - tig0031045:33240-33259 Msa1445370:three_prime_UTR 60.0%
CGGAGCTGGAGGAGGGTTTA+GGG - tig0031045:33241-33260 Msa1445370:three_prime_UTR 60.0%
GCTTCGTCGAAGAGCGGAAG+CGG - tig0031045:33299-33318 Msa1445370:three_prime_UTR 60.0%
GGCGAGTTCAAGACGAGTGG+TGG + tig0031045:33432-33451 None:intergenic 60.0%
GGTGGCGAGTTCAAGACGAG+TGG + tig0031045:33435-33454 None:intergenic 60.0%
GAGATCGTTGAGAGCGGTGG+TGG + tig0031045:33453-33472 None:intergenic 60.0%
TCGGAGCTGGAGGAGGGTTT+AGG - tig0031045:33240-33259 Msa1445370:three_prime_UTR 60.0%
CGGAGCTGGAGGAGGGTTTA+GGG - tig0031045:33241-33260 Msa1445370:three_prime_UTR 60.0%
GCTTCGTCGAAGAGCGGAAG+CGG - tig0031045:33299-33318 Msa1445370:three_prime_UTR 60.0%
GGCGAGTTCAAGACGAGTGG+TGG + tig0031045:33432-33451 None:intergenic 60.0%
GGTGGCGAGTTCAAGACGAG+TGG + tig0031045:33435-33454 None:intergenic 60.0%
GAGATCGTTGAGAGCGGTGG+TGG + tig0031045:33453-33472 None:intergenic 60.0%
CGGAAGCGGAGCGATGACTC+TGG - tig0031045:33313-33332 Msa1445370:three_prime_UTR 65.0%
! GCGGAGCGATGACTCTGGAG+AGG - tig0031045:33318-33337 Msa1445370:three_prime_UTR 65.0%
CGGAAGCGGAGCGATGACTC+TGG - tig0031045:33313-33332 Msa1445370:three_prime_UTR 65.0%
! GCGGAGCGATGACTCTGGAG+AGG - tig0031045:33318-33337 Msa1445370:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
tig0031045 gene 33122 34662 33122 ID=Msa1445370;Name=Msa1445370
tig0031045 mRNA 33122 34662 33122 ID=Msa1445370-mRNA-1;Parent=Msa1445370;Name=Msa1445370-mRNA-1;_AED=0.01;_eAED=0.01;_QI=174|1|1|1|1|1|3|482|152
tig0031045 exon 33122 33687 33122 ID=Msa1445370-mRNA-1:exon:14956;Parent=Msa1445370-mRNA-1
tig0031045 exon 34001 34078 34001 ID=Msa1445370-mRNA-1:exon:14955;Parent=Msa1445370-mRNA-1
tig0031045 exon 34192 34662 34192 ID=Msa1445370-mRNA-1:exon:14954;Parent=Msa1445370-mRNA-1
tig0031045 five_prime_UTR 34489 34662 34489 ID=Msa1445370-mRNA-1:five_prime_utr;Parent=Msa1445370-mRNA-1
tig0031045 CDS 34192 34488 34192 ID=Msa1445370-mRNA-1:cds;Parent=Msa1445370-mRNA-1
tig0031045 CDS 34001 34078 34001 ID=Msa1445370-mRNA-1:cds;Parent=Msa1445370-mRNA-1
tig0031045 CDS 33604 33687 33604 ID=Msa1445370-mRNA-1:cds;Parent=Msa1445370-mRNA-1
tig0031045 three_prime_UTR 33122 33603 33122 ID=Msa1445370-mRNA-1:three_prime_utr;Parent=Msa1445370-mRNA-1
Gene Sequence

>Msa1445370

ATGGCTTCGTCGAAGAGCGGAAGCGGAGCGATGACTCTGGAGAGGATGAGCGTTGAACAGTTGAAAGCAGTTAAGGAACAAGCAGATATGGAAGTTAACCTTCTTCAAGACAGTCTCAACAATATCGGCACCGCCACCACTCGTCTTGAACTCGCCACCACCGCTCTCAACGATCTCTCTCTCCGTTCTCTCGGCAGTAAAATCCTTGTTCCTCTCACCGCTTCTCTCTATGTTCCTGCTACTCTTCAAGATCCTCATCACGTTCTCGTCGATATCGGAACTGGTTATTTCGTCGAGAAAACCATGCCTGAAGGAAAGGATTACTGCGAGCGCAAAATCAACTTACTCAAATCTAACTTCGATCAACTTGTTGAGGTTGCGTCCAAGAAGAAAAACGTTGCGGATGAAGCTGGGCTTATTTTACAGGCAAAATTGAAGCAATTAGCATCTTCATCCTAA
Protein sequence

>Msa1445370

MASSKSGSGAMTLERMSVEQLKAVKEQADMEVNLLQDSLNNIGTATTRLELATTALNDLSLRSLGSKILVPLTASLYVPATLQDPHHVLVDIGTGYFVEKTMPEGKDYCERKINLLKSNFDQLVEVASKKKNVADEAGLILQAKLKQLASSS*