AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1364480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364480 MtrunA17_Chr8g0383391 100.000 131 0 0 1 131 1 131 4.14e-95 270
Msa1364480 MtrunA17_Chr8g0382851 36.111 72 45 1 7 77 2 73 1.10e-12 61.2
Msa1364480 MtrunA17_Chr7g0269241 36.111 72 45 1 7 77 2 73 1.25e-12 60.8
Msa1364480 MtrunA17_Chr1g0195711 33.784 74 48 1 7 79 2 75 7.83e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364480 AT1G20580.1 84.091 132 19 2 1 131 1 131 1.14e-71 211
Msa1364480 AT1G76300.1 77.863 131 26 2 1 131 1 128 7.85e-69 203
Msa1364480 AT5G27720.1 36.111 72 45 1 7 77 2 73 2.56e-12 60.1

Find 55 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATCACTCTAAAATCTTT+TGG 0.274319 tig0018152:-25114 None:intergenic
GCTGGCCGTGGAGCTCCGCC+TGG 0.291361 tig0018152:+24738 Msa1364480:CDS
ATACTAATGTGACAGGTTTA+TGG 0.302035 tig0018152:+24373 Msa1364480:intron
TGATAGAAAGAAATTTGAAT+TGG 0.341162 tig0018152:+24856 Msa1364480:three_prime_UTR
AAAGAAGATGAGTCGTAGTT+TGG 0.344374 tig0018152:+22981 Msa1364480:exon
GCCTGTATTGTGTGCTTGGC+TGG 0.354352 tig0018152:+24925 Msa1364480:three_prime_UTR
TGATTGAGTGTGAGGATAAC+TGG 0.357866 tig0018152:+23088 Msa1364480:CDS
GAGTCGTAGTTTGGGAATTC+CGG 0.364238 tig0018152:+22990 Msa1364480:CDS
CCGTGGAGCTCCGCCTGGGA+GGG 0.368506 tig0018152:+24743 Msa1364480:CDS
CGTGGAGCTCCGCCTGGGAG+GGG 0.398677 tig0018152:+24744 Msa1364480:CDS
AGTTTGACAGCCCTCAAAAC+TGG 0.411974 tig0018152:+25024 Msa1364480:three_prime_UTR
TAGGCTCAAGCTGCTGGCCG+TGG 0.412883 tig0018152:+24726 Msa1364480:intron
AAGAAGATGAGTCGTAGTTT+GGG 0.420399 tig0018152:+22982 Msa1364480:exon
GCCGTGGAGCTCCGCCTGGG+AGG 0.444968 tig0018152:+24742 Msa1364480:CDS
CCTGTGTAGGCTCAAGCTGC+TGG 0.451467 tig0018152:+24720 Msa1364480:intron
CTTGAGCATGTATTTATTCG+AGG 0.458500 tig0018152:+24237 Msa1364480:CDS
GCCTCGTGGAGGAGCTTCAC+CGG 0.468346 tig0018152:-23009 None:intergenic
CTGGCCGTGGAGCTCCGCCT+GGG 0.468800 tig0018152:+24739 Msa1364480:CDS
TTATTCGAGGTAGCAAAGTC+AGG 0.472481 tig0018152:+24250 Msa1364480:CDS
TGCATCTAAACGCTTGAACA+TGG 0.482856 tig0018152:-24423 None:intergenic
GGAGCGTTCTTCAGCATGTC+GGG 0.484052 tig0018152:-24401 None:intergenic
GCATCGCTTGGTGTTGGTAG+AGG 0.487013 tig0018152:+24549 Msa1364480:CDS
ACCAGCCAAGCACACAATAC+AGG 0.498191 tig0018152:-24926 None:intergenic
AAGCTCCTCCACGAGGCCTC+CGG 0.500533 tig0018152:+23015 Msa1364480:CDS
AAGGGCAAGGGTGCATCGCT+TGG 0.512282 tig0018152:+24537 Msa1364480:intron
CCCTCCCAGGCGGAGCTCCA+CGG 0.537318 tig0018152:-24743 None:intergenic
AAGGGTGCATCGCTTGGTGT+TGG 0.544000 tig0018152:+24543 Msa1364480:CDS
AAGCATCACTTACACTGCTA+AGG 0.553810 tig0018152:+23125 Msa1364480:CDS
GCGTTTAGATGCAAGAATCA+AGG 0.557683 tig0018152:+24433 Msa1364480:CDS
CTGAATATCTTGAAGGGCAA+GGG 0.558094 tig0018152:+24525 Msa1364480:intron
AATGATGATCACCTCCTCAC+TGG 0.558350 tig0018152:-24774 None:intergenic
CTCCACCGTGACGACGTGGC+CGG 0.561230 tig0018152:-23034 None:intergenic
GCATCTAAACGCTTGAACAT+GGG 0.565911 tig0018152:-24422 None:intergenic
ACTGGTGGTACACCCCTCCC+AGG 0.566465 tig0018152:-24756 None:intergenic
AGCAGTTGCAATGCGAGCAA+AGG 0.568696 tig0018152:+24575 Msa1364480:CDS
GGGAGCGTTCTTCAGCATGT+CGG 0.573337 tig0018152:-24402 None:intergenic
CCCTCAAAACTGGTTTAAGA+CGG 0.578521 tig0018152:+25034 Msa1364480:three_prime_UTR
TGTAAGTGATGCTTTCGAGT+TGG 0.586493 tig0018152:-23116 None:intergenic
GGCCTCCGGCCACGTCGTCA+CGG 0.591892 tig0018152:+23029 Msa1364480:CDS
CGACGTGGCCGGAGGCCTCG+TGG 0.593417 tig0018152:-23023 None:intergenic
CACCGTGACGACGTGGCCGG+AGG 0.597159 tig0018152:-23031 None:intergenic
ATTCACAGACTACTTCTCAC+TGG 0.604652 tig0018152:+24890 Msa1364480:three_prime_UTR
AGGCAACATGATTGAGTGTG+AGG 0.608133 tig0018152:+23080 Msa1364480:CDS
GTCACGGTGGAGCTCAAGAG+CGG 0.621698 tig0018152:+23045 Msa1364480:CDS
GGGGTGTACCACCAGTGAGG+AGG 0.633056 tig0018152:+24763 Msa1364480:CDS
AGTTATTATGAGTTTCGTGA+GGG 0.633783 tig0018152:+25000 Msa1364480:three_prime_UTR
AAGTTATTATGAGTTTCGTG+AGG 0.640457 tig0018152:+24999 Msa1364480:three_prime_UTR
GGAGGGGTGTACCACCAGTG+AGG 0.654099 tig0018152:+24760 Msa1364480:CDS
GATGATCACCTCCTCACTGG+TGG 0.658723 tig0018152:-24771 None:intergenic
TGAGCTCCACCGTGACGACG+TGG 0.674804 tig0018152:-23038 None:intergenic
CGTGGCCGGAGGCCTCGTGG+AGG 0.675507 tig0018152:-23020 None:intergenic
AAGAGCGGCGAACTCTACAG+AGG 0.686815 tig0018152:+23060 Msa1364480:CDS
TCCGGTGAAGCTCCTCCACG+AGG 0.694574 tig0018152:+23008 Msa1364480:CDS
GGTGGTACACCCCTCCCAGG+CGG 0.697960 tig0018152:-24753 None:intergenic
CTCCGGCCACGTCGTCACGG+TGG 0.732137 tig0018152:+23032 Msa1364480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATCACTTTTTAATAAGA+AGG - tig0018152:24461-24480 None:intergenic 15.0%
!! AATAAACTAGGAATATAAAA+AGG - tig0018152:24607-24626 None:intergenic 15.0%
!! TAGATGAAAATAAATAAACT+AGG - tig0018152:24619-24638 None:intergenic 15.0%
!!! CTTTTAAAATTAGTATATGA+CGG + tig0018152:24973-24992 Msa1364480:three_prime_UTR 15.0%
!!! TTAATCACTTTTTAATAAGA+AGG - tig0018152:24461-24480 None:intergenic 15.0%
!! AATAAACTAGGAATATAAAA+AGG - tig0018152:24607-24626 None:intergenic 15.0%
!! TAGATGAAAATAAATAAACT+AGG - tig0018152:24619-24638 None:intergenic 15.0%
!!! CTTTTAAAATTAGTATATGA+CGG + tig0018152:24973-24992 Msa1364480:three_prime_UTR 15.0%
!!! CATACATTTTTCACAATTTT+TGG + tig0018152:23188-23207 Msa1364480:intron 20.0%
!!! CTTTCTAGTATTAACTAATT+TGG + tig0018152:23343-23362 Msa1364480:intron 20.0%
!!! TACTTTTTTCTCTAATGATA+TGG - tig0018152:23381-23400 None:intergenic 20.0%
!! ATATCATTAGAGAAAAAAGT+AGG + tig0018152:23380-23399 Msa1364480:intron 20.0%
!! TATCATTAGAGAAAAAAGTA+GGG + tig0018152:23381-23400 Msa1364480:intron 20.0%
!!! ACAAGTAATTTTGTTAAAGA+GGG + tig0018152:23446-23465 Msa1364480:intron 20.0%
!! GAAAAAAAAAGTTACAAAAC+AGG - tig0018152:23563-23582 None:intergenic 20.0%
!!! CATGTCAATTCTTATTTATA+AGG + tig0018152:23786-23805 Msa1364480:intron 20.0%
!! AATAAATTAACAGTAAGACA+TGG + tig0018152:23964-23983 Msa1364480:intron 20.0%
!! AAGAAATTAACAAGTTTCAT+AGG - tig0018152:24099-24118 None:intergenic 20.0%
!!! TAACGTAATTATTTCAAGTA+AGG + tig0018152:24305-24324 Msa1364480:intron 20.0%
!! GTGATTAATTACTCTATATT+TGG + tig0018152:24473-24492 Msa1364480:intron 20.0%
!! TGATAGAAAGAAATTTGAAT+TGG + tig0018152:24856-24875 Msa1364480:three_prime_UTR 20.0%
!!! CTAGTTATTTTGTAATTAGA+AGG + tig0018152:25084-25103 Msa1364480:three_prime_UTR 20.0%
!!! CATACATTTTTCACAATTTT+TGG + tig0018152:23188-23207 Msa1364480:intron 20.0%
!!! CTTTCTAGTATTAACTAATT+TGG + tig0018152:23343-23362 Msa1364480:intron 20.0%
!!! TACTTTTTTCTCTAATGATA+TGG - tig0018152:23381-23400 None:intergenic 20.0%
!! ATATCATTAGAGAAAAAAGT+AGG + tig0018152:23380-23399 Msa1364480:intron 20.0%
!! TATCATTAGAGAAAAAAGTA+GGG + tig0018152:23381-23400 Msa1364480:intron 20.0%
!!! ACAAGTAATTTTGTTAAAGA+GGG + tig0018152:23446-23465 Msa1364480:intron 20.0%
!! GAAAAAAAAAGTTACAAAAC+AGG - tig0018152:23563-23582 None:intergenic 20.0%
!!! CATGTCAATTCTTATTTATA+AGG + tig0018152:23786-23805 Msa1364480:intron 20.0%
!! AATAAATTAACAGTAAGACA+TGG + tig0018152:23964-23983 Msa1364480:intron 20.0%
!! AAGAAATTAACAAGTTTCAT+AGG - tig0018152:24099-24118 None:intergenic 20.0%
!!! TAACGTAATTATTTCAAGTA+AGG + tig0018152:24305-24324 Msa1364480:intron 20.0%
!! GTGATTAATTACTCTATATT+TGG + tig0018152:24473-24492 Msa1364480:intron 20.0%
!! TGATAGAAAGAAATTTGAAT+TGG + tig0018152:24856-24875 Msa1364480:three_prime_UTR 20.0%
!!! CTAGTTATTTTGTAATTAGA+AGG + tig0018152:25084-25103 Msa1364480:three_prime_UTR 20.0%
! AAAAAACTGTGTATTAGAAG+AGG + tig0018152:22913-22932 Msa1364480:five_prime_UTR 25.0%
! TTGAAAAAGATCGTGAATTA+GGG + tig0018152:22941-22960 Msa1364480:five_prime_UTR 25.0%
!! TAGGGTTTATGTTGTATTTT+AGG + tig0018152:23286-23305 Msa1364480:intron 25.0%
!! AGGGTTTATGTTGTATTTTA+GGG + tig0018152:23287-23306 Msa1364480:intron 25.0%
!! AGAAAAAAGTAGGGATTTTT+GGG + tig0018152:23390-23409 Msa1364480:intron 25.0%
!! CAAAATTACTTGTGCTTTTA+TGG - tig0018152:23439-23458 None:intergenic 25.0%
!! CACAAGTAATTTTGTTAAAG+AGG + tig0018152:23445-23464 Msa1364480:intron 25.0%
!!! ATCAAAAGCTTTAAGCTATA+TGG - tig0018152:23657-23676 None:intergenic 25.0%
!! TATAGCTTAAAGCTTTTGAT+AGG + tig0018152:23657-23676 Msa1364480:intron 25.0%
!!! TTACTTTTTCCAAGATGAAA+GGG - tig0018152:23743-23762 None:intergenic 25.0%
!!! TTTACTTTTTCCAAGATGAA+AGG - tig0018152:23744-23763 None:intergenic 25.0%
!!! AAATCTCTCTTAATGGATTA+AGG - tig0018152:23913-23932 None:intergenic 25.0%
! TTAATCCATTAAGAGAGATT+TGG + tig0018152:23912-23931 Msa1364480:intron 25.0%
! TAATCCATTAAGAGAGATTT+GGG + tig0018152:23913-23932 Msa1364480:intron 25.0%
! TCTACAATAGTTGCTAATAA+TGG + tig0018152:24181-24200 Msa1364480:intron 25.0%
!! TTTTTGTCTGAATATCTTGA+AGG + tig0018152:24518-24537 Msa1364480:intron 25.0%
!! TTTTGTCTGAATATCTTGAA+GGG + tig0018152:24519-24538 Msa1364480:intron 25.0%
!!! TATTTATTTTCATCTAGCCT+TGG + tig0018152:24623-24642 Msa1364480:intron 25.0%
!!! ATTTATTTTCATCTAGCCTT+GGG + tig0018152:24624-24643 Msa1364480:intron 25.0%
! AAAAAACTGTGTATTAGAAG+AGG + tig0018152:22913-22932 Msa1364480:five_prime_UTR 25.0%
! TTGAAAAAGATCGTGAATTA+GGG + tig0018152:22941-22960 Msa1364480:five_prime_UTR 25.0%
!! TAGGGTTTATGTTGTATTTT+AGG + tig0018152:23286-23305 Msa1364480:intron 25.0%
!! AGGGTTTATGTTGTATTTTA+GGG + tig0018152:23287-23306 Msa1364480:intron 25.0%
!! AGAAAAAAGTAGGGATTTTT+GGG + tig0018152:23390-23409 Msa1364480:intron 25.0%
!! CAAAATTACTTGTGCTTTTA+TGG - tig0018152:23439-23458 None:intergenic 25.0%
!! CACAAGTAATTTTGTTAAAG+AGG + tig0018152:23445-23464 Msa1364480:intron 25.0%
!!! ATCAAAAGCTTTAAGCTATA+TGG - tig0018152:23657-23676 None:intergenic 25.0%
!! TATAGCTTAAAGCTTTTGAT+AGG + tig0018152:23657-23676 Msa1364480:intron 25.0%
!!! TTACTTTTTCCAAGATGAAA+GGG - tig0018152:23743-23762 None:intergenic 25.0%
!!! TTTACTTTTTCCAAGATGAA+AGG - tig0018152:23744-23763 None:intergenic 25.0%
!!! AAATCTCTCTTAATGGATTA+AGG - tig0018152:23913-23932 None:intergenic 25.0%
! TTAATCCATTAAGAGAGATT+TGG + tig0018152:23912-23931 Msa1364480:intron 25.0%
! TAATCCATTAAGAGAGATTT+GGG + tig0018152:23913-23932 Msa1364480:intron 25.0%
! TCTACAATAGTTGCTAATAA+TGG + tig0018152:24181-24200 Msa1364480:intron 25.0%
!! TTTTTGTCTGAATATCTTGA+AGG + tig0018152:24518-24537 Msa1364480:intron 25.0%
!! TTTTGTCTGAATATCTTGAA+GGG + tig0018152:24519-24538 Msa1364480:intron 25.0%
!!! TATTTATTTTCATCTAGCCT+TGG + tig0018152:24623-24642 Msa1364480:intron 25.0%
!!! ATTTATTTTCATCTAGCCTT+GGG + tig0018152:24624-24643 Msa1364480:intron 25.0%
GTTGAAAAAGATCGTGAATT+AGG + tig0018152:22940-22959 Msa1364480:five_prime_UTR 30.0%
!! TTTTTGGTAGAATTGAGTTG+TGG + tig0018152:23204-23223 Msa1364480:intron 30.0%
TAGGGATCTTTGAATACTTA+TGG + tig0018152:23305-23324 Msa1364480:intron 30.0%
!!! ATACTTATGGTTTTGAACAC+TGG + tig0018152:23318-23337 Msa1364480:intron 30.0%
! GAGAAAAAAGTAGGGATTTT+TGG + tig0018152:23389-23408 Msa1364480:intron 30.0%
! GCTTTTATGGATCATATACA+TGG - tig0018152:23426-23445 None:intergenic 30.0%
TAAATCGACCCATAAAAGTT+AGG + tig0018152:23475-23494 Msa1364480:intron 30.0%
! AAACAGAGCCTAACTTTTAT+GGG - tig0018152:23486-23505 None:intergenic 30.0%
! AAAACAGAGCCTAACTTTTA+TGG - tig0018152:23487-23506 None:intergenic 30.0%
!!! GTTACAAAACAGGTCTTTTT+TGG - tig0018152:23553-23572 None:intergenic 30.0%
TATCAAACTAAGCAATAAGC+TGG - tig0018152:23620-23639 None:intergenic 30.0%
!!! TACTTTTTCCAAGATGAAAG+GGG - tig0018152:23742-23761 None:intergenic 30.0%
TGGATTGAATTTGTAGTGTT+TGG - tig0018152:23832-23851 None:intergenic 30.0%
!! TAATGGATTAAGGCTTTGTT+TGG - tig0018152:23903-23922 None:intergenic 30.0%
CACTTGAAATGAAGATACTA+AGG - tig0018152:24137-24156 None:intergenic 30.0%
! TGTATATCGCATTTTCTGTA+AGG + tig0018152:24334-24353 Msa1364480:intron 30.0%
ATACTAATGTGACAGGTTTA+TGG + tig0018152:24373-24392 Msa1364480:intron 30.0%
! ATTGAACAAACATTTTCCCA+AGG - tig0018152:24643-24662 None:intergenic 30.0%
TTACACCACATTTGTAACAA+CGG - tig0018152:24688-24707 None:intergenic 30.0%
! AATCTCTATTTTCTGTCTGT+TGG + tig0018152:24802-24821 Msa1364480:three_prime_UTR 30.0%
AAGTTATTATGAGTTTCGTG+AGG + tig0018152:24999-25018 Msa1364480:three_prime_UTR 30.0%
AGTTATTATGAGTTTCGTGA+GGG + tig0018152:25000-25019 Msa1364480:three_prime_UTR 30.0%
GTTGAAAAAGATCGTGAATT+AGG + tig0018152:22940-22959 Msa1364480:five_prime_UTR 30.0%
!! TTTTTGGTAGAATTGAGTTG+TGG + tig0018152:23204-23223 Msa1364480:intron 30.0%
TAGGGATCTTTGAATACTTA+TGG + tig0018152:23305-23324 Msa1364480:intron 30.0%
!!! ATACTTATGGTTTTGAACAC+TGG + tig0018152:23318-23337 Msa1364480:intron 30.0%
! GAGAAAAAAGTAGGGATTTT+TGG + tig0018152:23389-23408 Msa1364480:intron 30.0%
! GCTTTTATGGATCATATACA+TGG - tig0018152:23426-23445 None:intergenic 30.0%
TAAATCGACCCATAAAAGTT+AGG + tig0018152:23475-23494 Msa1364480:intron 30.0%
! AAACAGAGCCTAACTTTTAT+GGG - tig0018152:23486-23505 None:intergenic 30.0%
! AAAACAGAGCCTAACTTTTA+TGG - tig0018152:23487-23506 None:intergenic 30.0%
!!! GTTACAAAACAGGTCTTTTT+TGG - tig0018152:23553-23572 None:intergenic 30.0%
TATCAAACTAAGCAATAAGC+TGG - tig0018152:23620-23639 None:intergenic 30.0%
!!! TACTTTTTCCAAGATGAAAG+GGG - tig0018152:23742-23761 None:intergenic 30.0%
TGGATTGAATTTGTAGTGTT+TGG - tig0018152:23832-23851 None:intergenic 30.0%
!! TAATGGATTAAGGCTTTGTT+TGG - tig0018152:23903-23922 None:intergenic 30.0%
CACTTGAAATGAAGATACTA+AGG - tig0018152:24137-24156 None:intergenic 30.0%
! TGTATATCGCATTTTCTGTA+AGG + tig0018152:24334-24353 Msa1364480:intron 30.0%
ATACTAATGTGACAGGTTTA+TGG + tig0018152:24373-24392 Msa1364480:intron 30.0%
! ATTGAACAAACATTTTCCCA+AGG - tig0018152:24643-24662 None:intergenic 30.0%
TTACACCACATTTGTAACAA+CGG - tig0018152:24688-24707 None:intergenic 30.0%
! AATCTCTATTTTCTGTCTGT+TGG + tig0018152:24802-24821 Msa1364480:three_prime_UTR 30.0%
AAGTTATTATGAGTTTCGTG+AGG + tig0018152:24999-25018 Msa1364480:three_prime_UTR 30.0%
AGTTATTATGAGTTTCGTGA+GGG + tig0018152:25000-25019 Msa1364480:three_prime_UTR 30.0%
AAAGAAGATGAGTCGTAGTT+TGG + tig0018152:22981-23000 Msa1364480:exon 35.0%
AAGAAGATGAGTCGTAGTTT+GGG + tig0018152:22982-23001 Msa1364480:exon 35.0%
!! AAAAAGTAGGGATTTTTGGG+TGG + tig0018152:23393-23412 Msa1364480:intron 35.0%
ACAACCCAAATCTCTCTTAA+TGG - tig0018152:23920-23939 None:intergenic 35.0%
!! TAAGAGAGATTTGGGTTGTA+TGG + tig0018152:23921-23940 Msa1364480:intron 35.0%
GAAAAAGTCTTTGGTTGTTG+TGG + tig0018152:24023-24042 Msa1364480:intron 35.0%
AGTTTCATAGGAGAGAAAGA+TGG - tig0018152:24087-24106 None:intergenic 35.0%
ATAATGGCTAAATCTTCTGC+AGG + tig0018152:24197-24216 Msa1364480:intron 35.0%
CTTGAGCATGTATTTATTCG+AGG + tig0018152:24237-24256 Msa1364480:CDS 35.0%
TGAAGTGATACTAATGTGAC+AGG + tig0018152:24366-24385 Msa1364480:intron 35.0%
!! TTCAATGACTTGCTAGTTGA+AGG + tig0018152:24657-24676 Msa1364480:intron 35.0%
! GTAGAATTTTGTCTTAGGTG+TGG + tig0018152:24947-24966 Msa1364480:three_prime_UTR 35.0%
AAAGAAGATGAGTCGTAGTT+TGG + tig0018152:22981-23000 Msa1364480:exon 35.0%
AAGAAGATGAGTCGTAGTTT+GGG + tig0018152:22982-23001 Msa1364480:exon 35.0%
!! AAAAAGTAGGGATTTTTGGG+TGG + tig0018152:23393-23412 Msa1364480:intron 35.0%
ACAACCCAAATCTCTCTTAA+TGG - tig0018152:23920-23939 None:intergenic 35.0%
!! TAAGAGAGATTTGGGTTGTA+TGG + tig0018152:23921-23940 Msa1364480:intron 35.0%
GAAAAAGTCTTTGGTTGTTG+TGG + tig0018152:24023-24042 Msa1364480:intron 35.0%
AGTTTCATAGGAGAGAAAGA+TGG - tig0018152:24087-24106 None:intergenic 35.0%
ATAATGGCTAAATCTTCTGC+AGG + tig0018152:24197-24216 Msa1364480:intron 35.0%
CTTGAGCATGTATTTATTCG+AGG + tig0018152:24237-24256 Msa1364480:CDS 35.0%
TGAAGTGATACTAATGTGAC+AGG + tig0018152:24366-24385 Msa1364480:intron 35.0%
!! TTCAATGACTTGCTAGTTGA+AGG + tig0018152:24657-24676 Msa1364480:intron 35.0%
! GTAGAATTTTGTCTTAGGTG+TGG + tig0018152:24947-24966 Msa1364480:three_prime_UTR 35.0%
TGATTGAGTGTGAGGATAAC+TGG + tig0018152:23088-23107 Msa1364480:CDS 40.0%
! TGTAAGTGATGCTTTCGAGT+TGG - tig0018152:23119-23138 None:intergenic 40.0%
AAGCATCACTTACACTGCTA+AGG + tig0018152:23125-23144 Msa1364480:CDS 40.0%
CGTTGTGATGTTCGCAAATT+TGG + tig0018152:23246-23265 Msa1364480:intron 40.0%
!!! TAGGGATTTTTGGGTGGTTT+CGG + tig0018152:23399-23418 Msa1364480:intron 40.0%
CAAAGTTACCCCTTTCATCT+TGG + tig0018152:23731-23750 Msa1364480:intron 40.0%
CTAGCTTATAGCAGATAAGC+TGG - tig0018152:23855-23874 None:intergenic 40.0%
! TTTTTCCCACCAAGATAGAC+GGG - tig0018152:24009-24028 None:intergenic 40.0%
! CTTTTTCCCACCAAGATAGA+CGG - tig0018152:24010-24029 None:intergenic 40.0%
TCTTGGTGGGAAAAAGTCTT+TGG + tig0018152:24014-24033 Msa1364480:intron 40.0%
TTATTCGAGGTAGCAAAGTC+AGG + tig0018152:24250-24269 Msa1364480:CDS 40.0%
GCATCTAAACGCTTGAACAT+GGG - tig0018152:24425-24444 None:intergenic 40.0%
TGCATCTAAACGCTTGAACA+TGG - tig0018152:24426-24445 None:intergenic 40.0%
GCGTTTAGATGCAAGAATCA+AGG + tig0018152:24433-24452 Msa1364480:CDS 40.0%
TCTGAATATCTTGAAGGGCA+AGG + tig0018152:24524-24543 Msa1364480:intron 40.0%
CTGAATATCTTGAAGGGCAA+GGG + tig0018152:24525-24544 Msa1364480:intron 40.0%
ACCTACCGTTGTTACAAATG+TGG + tig0018152:24680-24699 Msa1364480:intron 40.0%
ACCACATTTGTAACAACGGT+AGG - tig0018152:24684-24703 None:intergenic 40.0%
ACCTCAAATGTTTCCTGTGT+AGG + tig0018152:24707-24726 Msa1364480:intron 40.0%
ATTCACAGACTACTTCTCAC+TGG + tig0018152:24890-24909 Msa1364480:three_prime_UTR 40.0%
! TTTTGCCTGTATTGTGTGCT+TGG + tig0018152:24921-24940 Msa1364480:three_prime_UTR 40.0%
! GGCTGGTAGAATTTTGTCTT+AGG + tig0018152:24942-24961 Msa1364480:three_prime_UTR 40.0%
!! CCGTCTTAAACCAGTTTTGA+GGG - tig0018152:25037-25056 None:intergenic 40.0%
! CCCTCAAAACTGGTTTAAGA+CGG + tig0018152:25034-25053 Msa1364480:three_prime_UTR 40.0%
! TCCGTCTTAAACCAGTTTTG+AGG - tig0018152:25038-25057 None:intergenic 40.0%
TGATTGAGTGTGAGGATAAC+TGG + tig0018152:23088-23107 Msa1364480:CDS 40.0%
! TGTAAGTGATGCTTTCGAGT+TGG - tig0018152:23119-23138 None:intergenic 40.0%
AAGCATCACTTACACTGCTA+AGG + tig0018152:23125-23144 Msa1364480:CDS 40.0%
CGTTGTGATGTTCGCAAATT+TGG + tig0018152:23246-23265 Msa1364480:intron 40.0%
!!! TAGGGATTTTTGGGTGGTTT+CGG + tig0018152:23399-23418 Msa1364480:intron 40.0%
CAAAGTTACCCCTTTCATCT+TGG + tig0018152:23731-23750 Msa1364480:intron 40.0%
CTAGCTTATAGCAGATAAGC+TGG - tig0018152:23855-23874 None:intergenic 40.0%
! TTTTTCCCACCAAGATAGAC+GGG - tig0018152:24009-24028 None:intergenic 40.0%
! CTTTTTCCCACCAAGATAGA+CGG - tig0018152:24010-24029 None:intergenic 40.0%
TCTTGGTGGGAAAAAGTCTT+TGG + tig0018152:24014-24033 Msa1364480:intron 40.0%
TTATTCGAGGTAGCAAAGTC+AGG + tig0018152:24250-24269 Msa1364480:CDS 40.0%
GCATCTAAACGCTTGAACAT+GGG - tig0018152:24425-24444 None:intergenic 40.0%
TGCATCTAAACGCTTGAACA+TGG - tig0018152:24426-24445 None:intergenic 40.0%
GCGTTTAGATGCAAGAATCA+AGG + tig0018152:24433-24452 Msa1364480:CDS 40.0%
TCTGAATATCTTGAAGGGCA+AGG + tig0018152:24524-24543 Msa1364480:intron 40.0%
CTGAATATCTTGAAGGGCAA+GGG + tig0018152:24525-24544 Msa1364480:intron 40.0%
ACCTACCGTTGTTACAAATG+TGG + tig0018152:24680-24699 Msa1364480:intron 40.0%
ACCACATTTGTAACAACGGT+AGG - tig0018152:24684-24703 None:intergenic 40.0%
ACCTCAAATGTTTCCTGTGT+AGG + tig0018152:24707-24726 Msa1364480:intron 40.0%
ATTCACAGACTACTTCTCAC+TGG + tig0018152:24890-24909 Msa1364480:three_prime_UTR 40.0%
! TTTTGCCTGTATTGTGTGCT+TGG + tig0018152:24921-24940 Msa1364480:three_prime_UTR 40.0%
! GGCTGGTAGAATTTTGTCTT+AGG + tig0018152:24942-24961 Msa1364480:three_prime_UTR 40.0%
!! CCGTCTTAAACCAGTTTTGA+GGG - tig0018152:25037-25056 None:intergenic 40.0%
! CCCTCAAAACTGGTTTAAGA+CGG + tig0018152:25034-25053 Msa1364480:three_prime_UTR 40.0%
! TCCGTCTTAAACCAGTTTTG+AGG - tig0018152:25038-25057 None:intergenic 40.0%
GAGTCGTAGTTTGGGAATTC+CGG + tig0018152:22990-23009 Msa1364480:CDS 45.0%
AGGCAACATGATTGAGTGTG+AGG + tig0018152:23080-23099 Msa1364480:CDS 45.0%
GTTCGCAAATTTGGCAGATG+TGG + tig0018152:23255-23274 Msa1364480:intron 45.0%
CAAATTTGGCAGATGTGGAG+CGG + tig0018152:23260-23279 Msa1364480:intron 45.0%
AAATTTGGCAGATGTGGAGC+GGG + tig0018152:23261-23280 Msa1364480:intron 45.0%
GCTTATAGCAGATAAGCTGG+TGG - tig0018152:23852-23871 None:intergenic 45.0%
TGGCTAAATCTTCTGCAGGA+TGG + tig0018152:24201-24220 Msa1364480:intron 45.0%
GCCTACACAGGAAACATTTG+AGG - tig0018152:24711-24730 None:intergenic 45.0%
AATGATGATCACCTCCTCAC+TGG - tig0018152:24777-24796 None:intergenic 45.0%
AGTTTGACAGCCCTCAAAAC+TGG + tig0018152:25024-25043 Msa1364480:three_prime_UTR 45.0%
GAGTCGTAGTTTGGGAATTC+CGG + tig0018152:22990-23009 Msa1364480:CDS 45.0%
AGGCAACATGATTGAGTGTG+AGG + tig0018152:23080-23099 Msa1364480:CDS 45.0%
GTTCGCAAATTTGGCAGATG+TGG + tig0018152:23255-23274 Msa1364480:intron 45.0%
CAAATTTGGCAGATGTGGAG+CGG + tig0018152:23260-23279 Msa1364480:intron 45.0%
AAATTTGGCAGATGTGGAGC+GGG + tig0018152:23261-23280 Msa1364480:intron 45.0%
GCTTATAGCAGATAAGCTGG+TGG - tig0018152:23852-23871 None:intergenic 45.0%
TGGCTAAATCTTCTGCAGGA+TGG + tig0018152:24201-24220 Msa1364480:intron 45.0%
GCCTACACAGGAAACATTTG+AGG - tig0018152:24711-24730 None:intergenic 45.0%
AATGATGATCACCTCCTCAC+TGG - tig0018152:24777-24796 None:intergenic 45.0%
AGTTTGACAGCCCTCAAAAC+TGG + tig0018152:25024-25043 Msa1364480:three_prime_UTR 45.0%
AACGATAAACCCAGCCACGA+CGG - tig0018152:23230-23249 None:intergenic 50.0%
CTGATGTAGCCCGTCTATCT+TGG + tig0018152:23997-24016 Msa1364480:intron 50.0%
ATGTAGCCCGTCTATCTTGG+TGG + tig0018152:24000-24019 Msa1364480:intron 50.0%
TGTAGCCCGTCTATCTTGGT+GGG + tig0018152:24001-24020 Msa1364480:intron 50.0%
AGCAGTTGCAATGCGAGCAA+AGG + tig0018152:24575-24594 Msa1364480:CDS 50.0%
ACCAGCCAAGCACACAATAC+AGG - tig0018152:24929-24948 None:intergenic 50.0%
AACGATAAACCCAGCCACGA+CGG - tig0018152:23230-23249 None:intergenic 50.0%
CTGATGTAGCCCGTCTATCT+TGG + tig0018152:23997-24016 Msa1364480:intron 50.0%
ATGTAGCCCGTCTATCTTGG+TGG + tig0018152:24000-24019 Msa1364480:intron 50.0%
TGTAGCCCGTCTATCTTGGT+GGG + tig0018152:24001-24020 Msa1364480:intron 50.0%
AGCAGTTGCAATGCGAGCAA+AGG + tig0018152:24575-24594 Msa1364480:CDS 50.0%
ACCAGCCAAGCACACAATAC+AGG - tig0018152:24929-24948 None:intergenic 50.0%
AAGAGCGGCGAACTCTACAG+AGG + tig0018152:23060-23079 Msa1364480:CDS 55.0%
! GAATTGAGTTGTGGCCGTCG+TGG + tig0018152:23213-23232 Msa1364480:intron 55.0%
GCAGATGTGGAGCGGGATTA+GGG + tig0018152:23268-23287 Msa1364480:intron 55.0%
! GGAGCGTTCTTCAGCATGTC+GGG - tig0018152:24404-24423 None:intergenic 55.0%
!! GGGAGCGTTCTTCAGCATGT+CGG - tig0018152:24405-24424 None:intergenic 55.0%
!! AAGGGTGCATCGCTTGGTGT+TGG + tig0018152:24543-24562 Msa1364480:CDS 55.0%
!! GCATCGCTTGGTGTTGGTAG+AGG + tig0018152:24549-24568 Msa1364480:CDS 55.0%
GATGATCACCTCCTCACTGG+TGG - tig0018152:24774-24793 None:intergenic 55.0%
GCCTGTATTGTGTGCTTGGC+TGG + tig0018152:24925-24944 Msa1364480:three_prime_UTR 55.0%
AAGAGCGGCGAACTCTACAG+AGG + tig0018152:23060-23079 Msa1364480:CDS 55.0%
! GAATTGAGTTGTGGCCGTCG+TGG + tig0018152:23213-23232 Msa1364480:intron 55.0%
GCAGATGTGGAGCGGGATTA+GGG + tig0018152:23268-23287 Msa1364480:intron 55.0%
! GGAGCGTTCTTCAGCATGTC+GGG - tig0018152:24404-24423 None:intergenic 55.0%
!! GGGAGCGTTCTTCAGCATGT+CGG - tig0018152:24405-24424 None:intergenic 55.0%
!! AAGGGTGCATCGCTTGGTGT+TGG + tig0018152:24543-24562 Msa1364480:CDS 55.0%
!! GCATCGCTTGGTGTTGGTAG+AGG + tig0018152:24549-24568 Msa1364480:CDS 55.0%
GATGATCACCTCCTCACTGG+TGG - tig0018152:24774-24793 None:intergenic 55.0%
GCCTGTATTGTGTGCTTGGC+TGG + tig0018152:24925-24944 Msa1364480:three_prime_UTR 55.0%
GTCACGGTGGAGCTCAAGAG+CGG + tig0018152:23045-23064 Msa1364480:CDS 60.0%
GGCAGATGTGGAGCGGGATT+AGG + tig0018152:23267-23286 Msa1364480:intron 60.0%
! AAGGGCAAGGGTGCATCGCT+TGG + tig0018152:24537-24556 Msa1364480:intron 60.0%
CCAGCAGCTTGAGCCTACAC+AGG - tig0018152:24723-24742 None:intergenic 60.0%
CCTGTGTAGGCTCAAGCTGC+TGG + tig0018152:24720-24739 Msa1364480:intron 60.0%
GTCACGGTGGAGCTCAAGAG+CGG + tig0018152:23045-23064 Msa1364480:CDS 60.0%
GGCAGATGTGGAGCGGGATT+AGG + tig0018152:23267-23286 Msa1364480:intron 60.0%
! AAGGGCAAGGGTGCATCGCT+TGG + tig0018152:24537-24556 Msa1364480:intron 60.0%
CCAGCAGCTTGAGCCTACAC+AGG - tig0018152:24723-24742 None:intergenic 60.0%
CCTGTGTAGGCTCAAGCTGC+TGG + tig0018152:24720-24739 Msa1364480:intron 60.0%
TCCGGTGAAGCTCCTCCACG+AGG + tig0018152:23008-23027 Msa1364480:CDS 65.0%
GCCTCGTGGAGGAGCTTCAC+CGG - tig0018152:23012-23031 None:intergenic 65.0%
AAGCTCCTCCACGAGGCCTC+CGG + tig0018152:23015-23034 Msa1364480:CDS 65.0%
TGAGCTCCACCGTGACGACG+TGG - tig0018152:23041-23060 None:intergenic 65.0%
TGAGTTGTGGCCGTCGTGGC+TGG + tig0018152:23217-23236 Msa1364480:intron 65.0%
GAGTTGTGGCCGTCGTGGCT+GGG + tig0018152:23218-23237 Msa1364480:intron 65.0%
TAGGCTCAAGCTGCTGGCCG+TGG + tig0018152:24726-24745 Msa1364480:intron 65.0%
ACTGGTGGTACACCCCTCCC+AGG - tig0018152:24759-24778 None:intergenic 65.0%
! GGAGGGGTGTACCACCAGTG+AGG + tig0018152:24760-24779 Msa1364480:CDS 65.0%
GGGGTGTACCACCAGTGAGG+AGG + tig0018152:24763-24782 Msa1364480:CDS 65.0%
TCCGGTGAAGCTCCTCCACG+AGG + tig0018152:23008-23027 Msa1364480:CDS 65.0%
GCCTCGTGGAGGAGCTTCAC+CGG - tig0018152:23012-23031 None:intergenic 65.0%
AAGCTCCTCCACGAGGCCTC+CGG + tig0018152:23015-23034 Msa1364480:CDS 65.0%
TGAGCTCCACCGTGACGACG+TGG - tig0018152:23041-23060 None:intergenic 65.0%
TGAGTTGTGGCCGTCGTGGC+TGG + tig0018152:23217-23236 Msa1364480:intron 65.0%
GAGTTGTGGCCGTCGTGGCT+GGG + tig0018152:23218-23237 Msa1364480:intron 65.0%
TAGGCTCAAGCTGCTGGCCG+TGG + tig0018152:24726-24745 Msa1364480:intron 65.0%
ACTGGTGGTACACCCCTCCC+AGG - tig0018152:24759-24778 None:intergenic 65.0%
! GGAGGGGTGTACCACCAGTG+AGG + tig0018152:24760-24779 Msa1364480:CDS 65.0%
GGGGTGTACCACCAGTGAGG+AGG + tig0018152:24763-24782 Msa1364480:CDS 65.0%
CTCCACCGTGACGACGTGGC+CGG - tig0018152:23037-23056 None:intergenic 70.0%
GGTGGTACACCCCTCCCAGG+CGG - tig0018152:24756-24775 None:intergenic 70.0%
CTCCACCGTGACGACGTGGC+CGG - tig0018152:23037-23056 None:intergenic 70.0%
GGTGGTACACCCCTCCCAGG+CGG - tig0018152:24756-24775 None:intergenic 70.0%
GGCCTCCGGCCACGTCGTCA+CGG + tig0018152:23029-23048 Msa1364480:CDS 75.0%
CACCGTGACGACGTGGCCGG+AGG - tig0018152:23034-23053 None:intergenic 75.0%
CTCCGGCCACGTCGTCACGG+TGG + tig0018152:23032-23051 Msa1364480:CDS 75.0%
CTGGCCGTGGAGCTCCGCCT+GGG + tig0018152:24739-24758 Msa1364480:CDS 75.0%
CCCTCCCAGGCGGAGCTCCA+CGG - tig0018152:24746-24765 None:intergenic 75.0%
CCGTGGAGCTCCGCCTGGGA+GGG + tig0018152:24743-24762 Msa1364480:CDS 75.0%
CGTGGAGCTCCGCCTGGGAG+GGG + tig0018152:24744-24763 Msa1364480:CDS 75.0%
GGCCTCCGGCCACGTCGTCA+CGG + tig0018152:23029-23048 Msa1364480:CDS 75.0%
CACCGTGACGACGTGGCCGG+AGG - tig0018152:23034-23053 None:intergenic 75.0%
CTCCGGCCACGTCGTCACGG+TGG + tig0018152:23032-23051 Msa1364480:CDS 75.0%
CTGGCCGTGGAGCTCCGCCT+GGG + tig0018152:24739-24758 Msa1364480:CDS 75.0%
CCCTCCCAGGCGGAGCTCCA+CGG - tig0018152:24746-24765 None:intergenic 75.0%
CCGTGGAGCTCCGCCTGGGA+GGG + tig0018152:24743-24762 Msa1364480:CDS 75.0%
CGTGGAGCTCCGCCTGGGAG+GGG + tig0018152:24744-24763 Msa1364480:CDS 75.0%
! CGTGGCCGGAGGCCTCGTGG+AGG - tig0018152:23023-23042 None:intergenic 80.0%
! CGACGTGGCCGGAGGCCTCG+TGG - tig0018152:23026-23045 None:intergenic 80.0%
! GCTGGCCGTGGAGCTCCGCC+TGG + tig0018152:24738-24757 Msa1364480:CDS 80.0%
! GCCGTGGAGCTCCGCCTGGG+AGG + tig0018152:24742-24761 Msa1364480:CDS 80.0%
! CGTGGCCGGAGGCCTCGTGG+AGG - tig0018152:23023-23042 None:intergenic 80.0%
! CGACGTGGCCGGAGGCCTCG+TGG - tig0018152:23026-23045 None:intergenic 80.0%
! GCTGGCCGTGGAGCTCCGCC+TGG + tig0018152:24738-24757 Msa1364480:CDS 80.0%
! GCCGTGGAGCTCCGCCTGGG+AGG + tig0018152:24742-24761 Msa1364480:CDS 80.0%
Chromosome Type Strat End Strand Name
tig0018152 gene 22898 25125 22898 ID=Msa1364480;Name=Msa1364480
tig0018152 mRNA 22898 25125 22898 ID=Msa1364480-mRNA-1;Parent=Msa1364480;Name=Msa1364480-mRNA-1;_AED=0.13;_eAED=0.13;_QI=90|1|1|1|1|1|5|337|131
tig0018152 exon 22898 23146 22898 ID=Msa1364480-mRNA-1:exon:14035;Parent=Msa1364480-mRNA-1
tig0018152 exon 24219 24271 24219 ID=Msa1364480-mRNA-1:exon:14036;Parent=Msa1364480-mRNA-1
tig0018152 exon 24388 24454 24388 ID=Msa1364480-mRNA-1:exon:14037;Parent=Msa1364480-mRNA-1
tig0018152 exon 24540 24596 24540 ID=Msa1364480-mRNA-1:exon:14038;Parent=Msa1364480-mRNA-1
tig0018152 exon 24729 25125 24729 ID=Msa1364480-mRNA-1:exon:14039;Parent=Msa1364480-mRNA-1
tig0018152 five_prime_UTR 22898 22987 22898 ID=Msa1364480-mRNA-1:five_prime_utr;Parent=Msa1364480-mRNA-1
tig0018152 CDS 22988 23146 22988 ID=Msa1364480-mRNA-1:cds;Parent=Msa1364480-mRNA-1
tig0018152 CDS 24219 24271 24219 ID=Msa1364480-mRNA-1:cds;Parent=Msa1364480-mRNA-1
tig0018152 CDS 24388 24454 24388 ID=Msa1364480-mRNA-1:cds;Parent=Msa1364480-mRNA-1
tig0018152 CDS 24540 24596 24540 ID=Msa1364480-mRNA-1:cds;Parent=Msa1364480-mRNA-1
tig0018152 CDS 24729 24788 24729 ID=Msa1364480-mRNA-1:cds;Parent=Msa1364480-mRNA-1
tig0018152 three_prime_UTR 24789 25125 24789 ID=Msa1364480-mRNA-1:three_prime_utr;Parent=Msa1364480-mRNA-1
Gene Sequence

>Msa1364480

ATGAGTCGTAGTTTGGGAATTCCGGTGAAGCTCCTCCACGAGGCCTCCGGCCACGTCGTCACGGTGGAGCTCAAGAGCGGCGAACTCTACAGAGGCAACATGATTGAGTGTGAGGATAACTGGAACTGCCAACTCGAAAGCATCACTTACACTGCTAAGGATGGAAAAACGTCACAACTTGAGCATGTATTTATTCGAGGTAGCAAAGTCAGGTTTATGGTCATACCCGACATGCTGAAGAACGCTCCCATGTTCAAGCGTTTAGATGCAAGAATCAAGGGCAAGGGTGCATCGCTTGGTGTTGGTAGAGGCAGAGCAGTTGCAATGCGAGCAAAGGCTCAAGCTGCTGGCCGTGGAGCTCCGCCTGGGAGGGGTGTACCACCAGTGAGGAGGTGA
Protein sequence

>Msa1364480

MSRSLGIPVKLLHEASGHVVTVELKSGELYRGNMIECEDNWNCQLESITYTAKDGKTSQLEHVFIRGSKVRFMVIPDMLKNAPMFKRLDARIKGKGASLGVGRGRAVAMRAKAQAAGRGAPPGRGVPPVRR*