Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0112750 | Msa0203500 | 0.801213 | 1.000951e-48 | -8.615850e-47 |
| Msa0122590 | Msa0203500 | 0.819597 | 1.068509e-52 | -8.615850e-47 |
| Msa0203500 | Msa0233300 | 0.806954 | 6.400581e-50 | -8.615850e-47 |
| Msa0203500 | Msa0326200 | 0.825859 | 3.725622e-54 | -8.615850e-47 |
| Msa0203500 | Msa0353190 | 0.813711 | 2.228707e-51 | -8.615850e-47 |
| Msa0203500 | Msa0402290 | 0.802851 | 4.611289e-49 | -8.615850e-47 |
| Msa0203500 | Msa0456200 | 0.806993 | 6.280049e-50 | -8.615850e-47 |
| Msa0203500 | Msa0512900 | 0.801856 | 7.390202e-49 | -8.615850e-47 |
| Msa0203500 | Msa0627410 | 0.808601 | 2.859006e-50 | -8.615850e-47 |
| Msa0203500 | Msa0715470 | 0.804755 | 1.854750e-49 | -8.615850e-47 |
| Msa0203500 | Msa0741570 | 0.804951 | 1.688320e-49 | -8.615850e-47 |
| Msa0203500 | Msa0762360 | 0.801809 | 7.555399e-49 | -8.615850e-47 |
| Msa0203500 | Msa0766630 | 0.805147 | 1.536290e-49 | -8.615850e-47 |
| Msa0203500 | Msa0885290 | 0.805661 | 1.198713e-49 | -8.615850e-47 |
| Msa0203500 | Msa0887990 | 0.815652 | 8.285193e-52 | -8.615850e-47 |
| Msa0203500 | Msa1059570 | 0.810064 | 1.388139e-50 | -8.615850e-47 |
| Msa0203500 | Msa1081590 | 0.801851 | 7.407089e-49 | -8.615850e-47 |
| Msa0203500 | Msa1082500 | 0.803155 | 3.990188e-49 | -8.615850e-47 |
| Msa0203500 | Msa1329700 | 0.803201 | 3.903523e-49 | -8.615850e-47 |
| Msa0203500 | Msa1397610 | 0.818958 | 1.494087e-52 | -8.615850e-47 |
| Msa0203500 | Msa1411020 | 0.815666 | 8.223561e-52 | -8.615850e-47 |
| Msa0203500 | Msa1420140 | 0.815934 | 7.167747e-52 | -8.615850e-47 |
| Msa0203500 | Msa1420490 | 0.814391 | 1.578042e-51 | -8.615850e-47 |
| Msa0203500 | Msa1423390 | 0.812268 | 4.618385e-51 | -8.615850e-47 |
| Msa0203500 | Msa1424290 | 0.809064 | 2.275765e-50 | -8.615850e-47 |
| Msa0203500 | Msa1425100 | 0.806351 | 8.579420e-50 | -8.615850e-47 |
| Msa0203500 | Msa1434350 | 0.817241 | 3.652988e-52 | -8.615850e-47 |
| Msa0203500 | Msa1439320 | 0.819142 | 1.356139e-52 | -8.615850e-47 |
| Msa0203500 | Msa1440370 | 0.812353 | 4.423961e-51 | -8.615850e-47 |
| Msa0203500 | Msa1440810 | 0.812391 | 4.339848e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCCAAAGGTTTGCTAGTC+AGG | 0.266911 | 2_1:+27423952 | None:intergenic |
| CTCTTATAAGAAAACGTTGA+AGG | 0.403918 | 2_1:-27424251 | None:intergenic |
| GATCCTGACTAGCAAACCTT+TGG | 0.412531 | 2_1:-27423955 | Msa0203500:three_prime_UTR |
| TTTCCCAATGCCTTGCTAGT+TGG | 0.415166 | 2_1:+27424176 | None:intergenic |
| TGCTGCTATGGTGGAATCAT+TGG | 0.417275 | 2_1:-27423922 | Msa0203500:three_prime_UTR |
| GACTCCAACTAGCAAGGCAT+TGG | 0.479496 | 2_1:-27424180 | Msa0203500:CDS |
| TGGAGCTGATTCCACTGATA+AGG | 0.488659 | 2_1:-27424006 | Msa0203500:CDS |
| ATCCTGACTAGCAAACCTTT+GGG | 0.504155 | 2_1:-27423954 | Msa0203500:three_prime_UTR |
| GGGAAAGAGAGCTGCTGCTA+TGG | 0.514451 | 2_1:-27423934 | Msa0203500:three_prime_UTR |
| TAGCAAGGCATTGGGAAAGA+TGG | 0.517908 | 2_1:-27424171 | Msa0203500:CDS |
| ACTCCAACTAGCAAGGCATT+GGG | 0.530070 | 2_1:-27424179 | Msa0203500:CDS |
| AAAGAGAGCTGCTGCTATGG+TGG | 0.546142 | 2_1:-27423931 | Msa0203500:three_prime_UTR |
| AAAGATGGTTGTTTGTGAGC+TGG | 0.553610 | 2_1:-27424156 | Msa0203500:CDS |
| TATCAGTGGAATCAGCTCCA+TGG | 0.576037 | 2_1:+27424009 | None:intergenic |
| GCTGGCTAATCAAAATTTAG+CGG | 0.595667 | 2_1:-27424138 | Msa0203500:intron |
| AGGAGTTGATTCAAGTAACA+AGG | 0.598003 | 2_1:-27424231 | Msa0203500:CDS |
| GCAGCAGCTCTCTTTCCCAA+AGG | 0.600747 | 2_1:+27423939 | None:intergenic |
| AGCGACATCGTCCTTATCAG+TGG | 0.603044 | 2_1:+27423995 | None:intergenic |
| TGTTGTGACTCCAACTAGCA+AGG | 0.627219 | 2_1:-27424186 | Msa0203500:CDS |
| TAACAAGGACGAAGTTACTG+TGG | 0.689476 | 2_1:-27424216 | Msa0203500:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTCCTAAAACAAAAAAA+TGG | + | chr2_1:27424121-27424140 | None:intergenic | 15.0% |
| !!! | ATGCACCATTTTTTTGTTTT+AGG | - | chr2_1:27424113-27424132 | Msa0203500:intron | 25.0% |
| !!! | GTTTTAGGAAAAAAAATCCA+TGG | - | chr2_1:27424128-27424147 | Msa0203500:intron | 25.0% |
| ! | AGGAGTTGATTCAAGTAACA+AGG | - | chr2_1:27423923-27423942 | Msa0203500:three_prime_UTR | 35.0% |
| GCTGGCTAATCAAAATTTAG+CGG | - | chr2_1:27424016-27424035 | Msa0203500:CDS | 35.0% | |
| TAGTAGTAGCAACCATCTAA+AGG | - | chr2_1:27424089-27424108 | Msa0203500:intron | 35.0% | |
| AAATGGTGCATTCCTTTAGA+TGG | + | chr2_1:27424104-27424123 | None:intergenic | 35.0% | |
| TAACAAGGACGAAGTTACTG+TGG | - | chr2_1:27423938-27423957 | Msa0203500:three_prime_UTR | 40.0% | |
| AAAGATGGTTGTTTGTGAGC+TGG | - | chr2_1:27423998-27424017 | Msa0203500:CDS | 40.0% | |
| ATCCTGACTAGCAAACCTTT+GGG | - | chr2_1:27424200-27424219 | Msa0203500:CDS | 40.0% | |
| TGTTGTGACTCCAACTAGCA+AGG | - | chr2_1:27423968-27423987 | Msa0203500:three_prime_UTR | 45.0% | |
| ! | ACTCCAACTAGCAAGGCATT+GGG | - | chr2_1:27423975-27423994 | Msa0203500:exon | 45.0% |
| TTTCCCAATGCCTTGCTAGT+TGG | + | chr2_1:27423981-27424000 | None:intergenic | 45.0% | |
| !! | TAGCAAGGCATTGGGAAAGA+TGG | - | chr2_1:27423983-27424002 | Msa0203500:exon | 45.0% |
| TATCAGTGGAATCAGCTCCA+TGG | + | chr2_1:27424148-27424167 | None:intergenic | 45.0% | |
| ! | TGGAGCTGATTCCACTGATA+AGG | - | chr2_1:27424148-27424167 | Msa0203500:CDS | 45.0% |
| GATCCTGACTAGCAAACCTT+TGG | - | chr2_1:27424199-27424218 | Msa0203500:CDS | 45.0% | |
| !! | TTCCCAAAGGTTTGCTAGTC+AGG | + | chr2_1:27424205-27424224 | None:intergenic | 45.0% |
| ! | TGCTGCTATGGTGGAATCAT+TGG | - | chr2_1:27424232-27424251 | Msa0203500:CDS | 45.0% |
| GACTCCAACTAGCAAGGCAT+TGG | - | chr2_1:27423974-27423993 | Msa0203500:exon | 50.0% | |
| AGCGACATCGTCCTTATCAG+TGG | + | chr2_1:27424162-27424181 | None:intergenic | 50.0% | |
| AAAGAGAGCTGCTGCTATGG+TGG | - | chr2_1:27424223-27424242 | Msa0203500:CDS | 50.0% | |
| GCAGCAGCTCTCTTTCCCAA+AGG | + | chr2_1:27424218-27424237 | None:intergenic | 55.0% | |
| GGGAAAGAGAGCTGCTGCTA+TGG | - | chr2_1:27424220-27424239 | Msa0203500:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 27423915 | 27424261 | 27423915 | ID=Msa0203500;Name=Msa0203500 |
| chr2_1 | mRNA | 27423915 | 27424261 | 27423915 | ID=Msa0203500-mRNA-1;Parent=Msa0203500;Name=Msa0203500-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|1|0.5|1|1|1|2|76|57 |
| chr2_1 | exon | 27423915 | 27424041 | 27423915 | ID=Msa0203500-mRNA-1:exon:10137;Parent=Msa0203500-mRNA-1 |
| chr2_1 | exon | 27424139 | 27424261 | 27424139 | ID=Msa0203500-mRNA-1:exon:10136;Parent=Msa0203500-mRNA-1 |
| chr2_1 | CDS | 27424139 | 27424261 | 27424139 | ID=Msa0203500-mRNA-1:cds;Parent=Msa0203500-mRNA-1 |
| chr2_1 | CDS | 27423991 | 27424041 | 27423991 | ID=Msa0203500-mRNA-1:cds;Parent=Msa0203500-mRNA-1 |
| chr2_1 | three_prime_UTR | 27423915 | 27423990 | 27423915 | ID=Msa0203500-mRNA-1:three_prime_utr;Parent=Msa0203500-mRNA-1 |
| Gene Sequence |
| Protein sequence |
