AlfalfaGEDB | Msa1059570

M. sativa cultivar zhongmu-4 / Msa1059570

KEGG: K04733
GO: -
PFAM: PF01453.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1059570	sp\|Q9SXB4\|Y1130_ARATH	56.223	233	60	4	332	530	596	820	1.40e-68	239
Msa1059570	sp\|Q9SXB4\|Y1130_ARATH	41.304	230	124	5	125	354	268	486	2.68e-48	182
Msa1059570	sp\|Q9SXB4\|Y1130_ARATH	43.103	116	59	4	14	127	13	123	1.38e-17	90.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1059570	A0A2N9IGJ1	55.556	441	157	5	124	530	255	690	2.66e-155	469
Msa1059570	A0A2N9IGJ1	59.804	102	37	3	27	126	21	120	6.43e-23	114

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006070	Msa1059570	0.801699	7.959504e-49	-8.615850e-47
Msa0011170	Msa1059570	0.828528	8.549685e-55	-8.615850e-47
Msa0014710	Msa1059570	0.833041	6.696322e-56	-8.615850e-47
Msa0016570	Msa1059570	0.807727	4.388407e-50	-8.615850e-47
Msa0016580	Msa1059570	0.805443	1.331666e-49	-8.615850e-47
Msa0017070	Msa1059570	0.808223	3.441251e-50	-8.615850e-47
Msa0017510	Msa1059570	0.811599	6.458922e-51	-8.615850e-47
Msa0031340	Msa1059570	0.802217	6.229798e-49	-8.615850e-47
Msa0036140	Msa1059570	0.815172	1.059247e-51	-8.615850e-47
Msa0036920	Msa1059570	0.806042	9.969323e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1059570	MtrunA17_Chr7g0236121	88.785	214	24	0	123	336	262	475	2.01e-136	414
Msa1059570	MtrunA17_Chr7g0236121	81.279	219	6	2	332	516	592	809	3.10e-109	343
Msa1059570	MtrunA17_Chr7g0236121	93.548	124	8	0	1	124	1	124	1.19e-70	241
Msa1059570	MtrunA17_Chr7g0236201	82.403	233	6	2	332	530	558	789	7.92e-120	371
Msa1059570	MtrunA17_Chr7g0236201	88.202	178	21	0	123	300	262	439	2.04e-110	346
Msa1059570	MtrunA17_Chr7g0236201	93.548	124	8	0	1	124	1	124	7.58e-71	241
Msa1059570	MtrunA17_Chr7g0236161	88.202	178	21	0	123	300	262	439	1.20e-110	346
Msa1059570	MtrunA17_Chr7g0236161	78.392	199	29	2	332	516	558	756	1.40e-94	304
Msa1059570	MtrunA17_Chr7g0236161	93.548	124	8	0	1	124	1	124	5.13e-71	241
Msa1059570	MtrunA17_Chr4g0055011	65.236	233	46	2	332	530	159	390	5.42e-95	294

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1059570	AT1G11300.2	56.223	233	60	4	332	530	581	805	1.03e-69	239
Msa1059570	AT1G11300.2	39.130	230	114	5	125	354	268	471	9.01e-45	169
Msa1059570	AT1G11300.2	43.103	116	59	4	14	127	13	123	1.27e-18	90.1
Msa1059570	AT1G11300.1	56.223	233	60	4	332	530	596	820	1.42e-69	239
Msa1059570	AT1G11300.1	41.304	230	124	5	125	354	268	486	2.73e-49	182
Msa1059570	AT1G11300.1	43.103	116	59	4	14	127	13	123	1.41e-18	90.1
Msa1059570	AT1G11330.1	53.648	233	73	2	332	530	609	840	3.94e-68	235
Msa1059570	AT1G11330.1	48.571	175	82	4	150	319	297	468	1.18e-46	175
Msa1059570	AT1G11330.1	46.078	102	43	5	30	120	31	131	1.13e-15	80.9
Msa1059570	AT1G11330.2	53.648	233	73	2	332	530	611	842	4.13e-68	235

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Find 94 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGCTTTAGCATAATAGA+AGG	0.444365	7_2:+45961581	Msa1059570:CDS
AAAGTAAAATCCTTGATTGG+AGG	0.588823	7_2:+45961558	Msa1059570:CDS
AAATCTGATGTCTTTAGCTT+TGG	0.345102	7_2:+45961924	Msa1059570:CDS
AAATTGTGAAGAGAAATGGT+GGG	0.770178	7_2:+45960276	Msa1059570:CDS
AACATGGAATAGTCATGAAA+TGG	0.405106	7_2:+45960324	Msa1059570:CDS
AAGACGATATATTACCATGG+TGG	0.720058	7_2:-45960978	None:intergenic
AAGAGTGGTAACTTCACCTT+AGG	0.490821	7_2:+45959620	Msa1059570:CDS
AAGGAAATTGTGAAGAGAAA+TGG	0.446562	7_2:+45960272	Msa1059570:CDS
AATAGTCATGAAATGGAATG+TGG	0.662626	7_2:+45960331	Msa1059570:CDS
AATGATCTCGTGGTATTGAA+TGG	0.520056	7_2:+45959779	Msa1059570:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TACATCAACTCAGTTTATCA+AGG	+	chr7_2:45959577-45959596	Msa1059570:CDS	30.0%
AATTCTATGAATCGCTATGT+CGG	+	chr7_2:45959659-45959678	Msa1059570:CDS	30.0%
CACAACAGAAAGAACTATAT+GGG	+	chr7_2:45959898-45959917	Msa1059570:intron	30.0%
TTTAGCAACATTACGTTTGA+AGG	-	chr7_2:45959938-45959957	None:intergenic	30.0%
CTTACAAGCAACAAAATAAC+AGG	+	chr7_2:45959965-45959984	Msa1059570:intron	30.0%
AGAAAGTAAAACTCACATCA+TGG	+	chr7_2:45959990-45960009	Msa1059570:intron	30.0%
AGATAATGAGTTGTCATACA+TGG	-	chr7_2:45960154-45960173	None:intergenic	30.0%
TGTATGACAACTCATTATCT+CGG	+	chr7_2:45960154-45960173	Msa1059570:intron	30.0%
AAGGAAATTGTGAAGAGAAA+TGG	+	chr7_2:45960272-45960291	Msa1059570:CDS	30.0%
AAATTGTGAAGAGAAATGGT+GGG	+	chr7_2:45960276-45960295	Msa1059570:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	45959482	45962408	45959482	ID=Msa1059570;Name=Msa1059570
chr7_2	mRNA	45959482	45962408	45959482	ID=Msa1059570-mRNA-1;Parent=Msa1059570;Name=Msa1059570-mRNA-1;_AED=0.21;_eAED=0.22;_QI=0\|0.2\|0\|1\|1\|1\|6\|0\|530
chr7_2	exon	45959482	45959854	45959482	ID=Msa1059570-mRNA-1:exon:8336;Parent=Msa1059570-mRNA-1
chr7_2	exon	45960272	45960781	45960272	ID=Msa1059570-mRNA-1:exon:8337;Parent=Msa1059570-mRNA-1
chr7_2	exon	45960875	45960985	45960875	ID=Msa1059570-mRNA-1:exon:8338;Parent=Msa1059570-mRNA-1
chr7_2	exon	45961549	45961786	45961549	ID=Msa1059570-mRNA-1:exon:8339;Parent=Msa1059570-mRNA-1
chr7_2	exon	45961878	45962028	45961878	ID=Msa1059570-mRNA-1:exon:8340;Parent=Msa1059570-mRNA-1
chr7_2	exon	45962199	45962408	45962199	ID=Msa1059570-mRNA-1:exon:8341;Parent=Msa1059570-mRNA-1
chr7_2	CDS	45959482	45959854	45959482	ID=Msa1059570-mRNA-1:cds;Parent=Msa1059570-mRNA-1
chr7_2	CDS	45960272	45960781	45960272	ID=Msa1059570-mRNA-1:cds;Parent=Msa1059570-mRNA-1
chr7_2	CDS	45960875	45960985	45960875	ID=Msa1059570-mRNA-1:cds;Parent=Msa1059570-mRNA-1
chr7_2	CDS	45961549	45961786	45961549	ID=Msa1059570-mRNA-1:cds;Parent=Msa1059570-mRNA-1
chr7_2	CDS	45961878	45962028	45961878	ID=Msa1059570-mRNA-1:cds;Parent=Msa1059570-mRNA-1
chr7_2	CDS	45962199	45962408	45962199	ID=Msa1059570-mRNA-1:cds;Parent=Msa1059570-mRNA-1

Gene Sequence

>Msa1059570

ATGGATTTCAACAATTACAAAAATATGTTCATGTTGTTCATTTTGACCTTGCTGTGTTTTAATATTCTAGATGTTAGTGTTGCCCTAGACACCATTACATCAACTCAGTTTATCAAGGACCCTGAAACTCTAAGCTCCAAGAGTGGTAACTTCACCTTAGGCTTTTTTAGTCCTGAAAATTCTATGAATCGCTATGTCGGAATTTGGTGGCAGCCTCAATTCACAATCCTATGGGTGCTTAACAGAGACCAACCTCTCAAAGATTCTTCTGGTGTTGTTAAAATATCTGATAATGGTAATGATCTCGTGGTATTGAATGGAAAGAAAGAGGTGATTTGGACATCCAACGCACCGAATGTTGCAACCAATTCAAAAGGAAATTGTGAAGAGAAATGGTGGGATGAAAAGAAGAAAGAACGGGAAGTAACATGGAATAGTCATGAAATGGAATGTGGTGTTTATGGTGTATGTGGACATTTTGCAAGCTGTAATTCACAAAGTTCACCGATATGTAGCTGTTTGAAAGGGTTTGAGCCAAAGAACAAAGTGGAATGGAATAGAAAAAATTGGACTGAAGGGTGCGTTAGGAGGACACCTCTTCAACAGTGTGAAAGATACATAAATCAAAACACAAGGGAAGACAGTAACGCTGATGGTTTTTTGAAACTGTCGATGGTCAAAGTTCCAGATTTTGCAGACGGATCATCTCTCATACTGTCATCAGAAACATGCAGAAGTCAATGTCTGAAGAATTGCTCTTGTGTTGCATATTCTTATGATGCTGACATTGGTTGTATGTCTTGGACTGGAAATCTAGTCGACATACAACAATTCTCAAATGGAGGACTTGACTTGTATATTCGTGTAGCGCACACAGAACTTGATAAAGAGAGAAACATGACAGTCATCATCATTATTATAACTGTGCTAACAGGAACTGTCTTTGTTCTTGCTTGTGCGTATATCATGTGGAGAAGGAGAACTAACCACCATGATGCCTCGAAAAGTAAAATCCTTGATTGGAGGAAACGCTTTAGCATAATAGAAGGAATAGCTCGAGGATTGTTGTATCTGCACAGAGATTCCAGACTAAAAATTATACATAGAGATTTGAAGCCAAGTAATATCTTGCTAGATAATGAGCTTAATCCAAAAATATCAGACTTTGGTATGGCCAGAATCTTTGGAGGGAATGAAGATCAAGAAAATACAAGAAGAGTTGTTGGTACTTATGGTTATATGTCTCCAGAATATGCAATGCAAGGACTGTTTTCTGATAAATCTGATGTCTTTAGCTTTGGAGTTTTACTTCTTGAGATTATTAGTGGAAGACGAAATTCAAGTTTTTATGATTGCGAGAACTCTCTTACCCTTTTAGGATTTGTCCTATGTGTACAACAATTTTCCATAGACAGACCTGCCATGGCTGCTGTAATTTCTATGCTGAACAGTGACATTGTAGATCTTCCTCCTCCAAAGAAACCTGCATTCATCCTGAAGCAGAATATGCTGATCTCGGTGTCACCTGAAGAAAACAACGATGACTTGTACTCCGTCAACTTGGTCAGTATATCTGACATCCATGGCAGATAG

Protein sequence

>Msa1059570

MDFNNYKNMFMLFILTLLCFNILDVSVALDTITSTQFIKDPETLSSKSGNFTLGFFSPENSMNRYVGIWWQPQFTILWVLNRDQPLKDSSGVVKISDNGNDLVVLNGKKEVIWTSNAPNVATNSKGNCEEKWWDEKKKEREVTWNSHEMECGVYGVCGHFASCNSQSSPICSCLKGFEPKNKVEWNRKNWTEGCVRRTPLQQCERYINQNTREDSNADGFLKLSMVKVPDFADGSSLILSSETCRSQCLKNCSCVAYSYDADIGCMSWTGNLVDIQQFSNGGLDLYIRVAHTELDKERNMTVIIIIITVLTGTVFVLACAYIMWRRRTNHHDASKSKILDWRKRFSIIEGIARGLLYLHRDSRLKIIHRDLKPSNILLDNELNPKISDFGMARIFGGNEDQENTRRVVGTYGYMSPEYAMQGLFSDKSDVFSFGVLLLEIISGRRNSSFYDCENSLTLLGFVLCVQQFSIDRPAMAAVISMLNSDIVDLPPPKKPAFILKQNMLISVSPEENNDDLYSVNLVSISDIHGR*