AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0274530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0274530 MtrunA17_Chr2g0284111 90.106 283 11 1 1 266 1 283 0.0 509
Msa0274530 MtrunA17_Chr4g0045171 54.305 302 88 6 12 266 1 299 9.07e-102 298
Msa0274530 MtrunA17_Chr8g0379191 54.098 305 86 6 11 266 11 310 2.18e-100 295
Msa0274530 MtrunA17_Chr5g0436201 42.336 137 60 4 136 265 85 209 1.67e-29 110
Msa0274530 MtrunA17_Chr3g0112791 41.481 135 59 3 136 261 88 211 3.31e-29 110
Msa0274530 MtrunA17_Chr7g0240961 38.732 142 58 4 136 265 108 232 1.54e-26 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0274530 AT5G05830.1 40.000 155 73 4 119 265 59 201 3.70e-31 115
Msa0274530 AT2G34200.2 46.457 127 56 4 135 261 98 212 2.38e-28 108
Msa0274530 AT2G34200.1 46.457 127 56 4 135 261 98 212 2.38e-28 108
Msa0274530 AT5G01070.1 38.571 140 64 4 136 265 75 202 2.30e-25 100
Msa0274530 AT5G59000.1 43.269 104 59 0 163 266 127 230 1.47e-24 98.6
Msa0274530 AT2G37950.1 37.037 135 62 4 136 262 81 200 2.28e-24 97.4

Find 87 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTATTTCTTTCCATTATT+TGG 0.053941 2_3:-6373524 Msa0274530:three_prime_UTR
TTTCTCTCTGCATATGTTTC+AGG 0.123499 2_3:-6375916 Msa0274530:five_prime_UTR
AGGATTTGTCATCTGGCTTT+TGG 0.178740 2_3:-6375195 Msa0274530:CDS
ATGATGATGATTGGTTAAAA+AGG 0.236995 2_3:+6376054 None:intergenic
CTTGTCATCATCAATATTAT+AGG 0.248518 2_3:-6375883 Msa0274530:five_prime_UTR
AATAATGGAAAGAAATAATT+TGG 0.250141 2_3:+6373527 None:intergenic
TTCATTGAATTCAACTTGTT+TGG 0.256177 2_3:-6375965 Msa0274530:five_prime_UTR
TGTTGCTAGTAATGGATTTA+TGG 0.261113 2_3:-6373880 Msa0274530:CDS
TGATGAGAATTTGAGTAATT+TGG 0.267450 2_3:-6375553 Msa0274530:CDS
TGAAGTTAGAGTTGAAGTTT+TGG 0.306233 2_3:-6375595 Msa0274530:CDS
GTTCAGAGTGAGGGTGTTAA+TGG 0.314704 2_3:-6375384 Msa0274530:CDS
TTCTTTCTTATGTAGTCATT+TGG 0.314945 2_3:-6373330 Msa0274530:three_prime_UTR
TAGTTGTAATTCAAGCAATT+TGG 0.324157 2_3:-6375454 Msa0274530:CDS
GAAGCAAAAGCTGATGAATC+AGG 0.330581 2_3:-6375345 Msa0274530:CDS
TATACATATACTCCAAATAA+TGG 0.339424 2_3:+6373512 None:intergenic
GTTGAAGGGGTTGGAGAAGT+TGG 0.349114 2_3:-6375531 Msa0274530:CDS
GCCATTAAGAAATTACAAAA+TGG 0.360240 2_3:+6373780 None:intergenic
GGAGTATATGTATAAGTTGA+TGG 0.378246 2_3:-6373503 Msa0274530:three_prime_UTR
AGTAATTTGGTTGAAGGGGT+TGG 0.378420 2_3:-6375540 Msa0274530:CDS
TGAGGTGTGGTTCAAGCTTA+AGG 0.381572 2_3:-6375061 Msa0274530:CDS
GTGGAATGATAGAAGATTCA+TGG 0.388144 2_3:-6373853 Msa0274530:CDS
TGATGAATCAGGGCTTAGTT+TGG 0.391331 2_3:-6375334 Msa0274530:CDS
AAGATAAAACATGCGTGGTT+TGG 0.403032 2_3:+6376006 None:intergenic
TGTTAAGGTTTGAGAGTTGA+AGG 0.407475 2_3:-6375643 Msa0274530:five_prime_UTR
CATGTAGATATGTTCTAGCT+TGG 0.407674 2_3:-6373720 Msa0274530:exon
GGTTTGTAGGATTTGTCATC+TGG 0.411919 2_3:-6375202 Msa0274530:CDS
CAGCGAATTTCAAATCTTCT+CGG 0.412745 2_3:+6373589 None:intergenic
CCCGCAGGTTTGGTGGATGC+TGG 0.418856 2_3:-6373812 Msa0274530:CDS
ATGTAGATATGTTCTAGCTT+GGG 0.420562 2_3:-6373719 Msa0274530:exon
TCATTCACTCTTATATTAGT+AGG 0.428728 2_3:+6373652 None:intergenic
GTTTCGGGTGTTGCTAGTAA+TGG 0.430988 2_3:-6373888 Msa0274530:CDS
GATAACTCATCCCGCAGGTT+TGG 0.431319 2_3:-6373822 Msa0274530:CDS
GGTTGATAAGTGTGATGAAT+TGG 0.440941 2_3:-6375496 Msa0274530:CDS
GTAGGTATGAAGAATTGAAA+AGG 0.442274 2_3:+6373670 None:intergenic
GGTTCAAGCTTAAGGGAAAC+AGG 0.442409 2_3:-6375053 Msa0274530:intron
TAGGTATGAAGAATTGAAAA+GGG 0.444173 2_3:+6373671 None:intergenic
AATATTATAGGTTACATACT+AGG 0.448065 2_3:-6375871 Msa0274530:five_prime_UTR
TAATTTCTTAATGGCGTGCC+TGG 0.448382 2_3:-6373772 Msa0274530:CDS
GAGGTGTGGTTCAAGCTTAA+GGG 0.458334 2_3:-6375060 Msa0274530:CDS
AATTTGAGTAATTTGGTTGA+AGG 0.460947 2_3:-6375546 Msa0274530:CDS
TCAGTTTCAGACACCCCTTT+CGG 0.465299 2_3:+6375297 None:intergenic
GGTAATATAGGAGCGGGGTT+CGG 0.467794 2_3:+6376076 None:intergenic
CACAAGGTGAAAGGGTTTGT+AGG 0.476227 2_3:-6375215 Msa0274530:CDS
AACTCATCCCGCAGGTTTGG+TGG 0.479462 2_3:-6373819 Msa0274530:CDS
TAAGTGTGATGAATTGGTTA+TGG 0.487167 2_3:-6375490 Msa0274530:CDS
AAACAGTAAAGAATTAGAAA+AGG 0.496102 2_3:+6376118 None:intergenic
TTTCGATGAAAGTGCCGAAA+GGG 0.498203 2_3:-6375311 Msa0274530:CDS
TGAAACATATGCAGAGAGAA+AGG 0.499079 2_3:+6375918 None:intergenic
AAATTGAGAATGATGATGAT+TGG 0.500097 2_3:+6376045 None:intergenic
TGAGAGCTCACAAGGTGAAA+GGG 0.506580 2_3:-6375223 Msa0274530:CDS
ATTTGAGTAATTTGGTTGAA+GGG 0.507847 2_3:-6375545 Msa0274530:CDS
ATGAGAGCTCACAAGGTGAA+AGG 0.513120 2_3:-6375224 Msa0274530:CDS
CCAGCATCCACCAAACCTGC+GGG 0.514150 2_3:+6373812 None:intergenic
GAAGTTACGAGCTTGATAAG+TGG 0.517301 2_3:-6373554 Msa0274530:three_prime_UTR
AGCTCTCATAAAACCCTTTG+TGG 0.534315 2_3:+6375238 None:intergenic
TTTGAGTAATTTGGTTGAAG+GGG 0.537818 2_3:-6375544 Msa0274530:CDS
GAATTGGTTATGGTTGATCA+AGG 0.552196 2_3:-6375480 Msa0274530:CDS
TAATAGCGTTCATTCTGCCA+TGG 0.558083 2_3:-6373749 Msa0274530:CDS
GAAACATATGCAGAGAGAAA+GGG 0.559305 2_3:+6375919 None:intergenic
AGGTTTGGTGGATGCTGGCG+AGG 0.561458 2_3:-6373807 Msa0274530:CDS
GTAATGGATTTATGGAAGAG+TGG 0.563387 2_3:-6373872 Msa0274530:CDS
AAATGACTACATAAGAAAGA+AGG 0.564888 2_3:+6373332 None:intergenic
TGAAGTGTAGCAGCCACAAA+GGG 0.566932 2_3:-6375251 Msa0274530:CDS
GCAGAATGAACGCTATTACC+AGG 0.567759 2_3:+6373754 None:intergenic
CTATAATATTGATGATGACA+AGG 0.568689 2_3:+6375884 None:intergenic
GCCAGCATCCACCAAACCTG+CGG 0.569525 2_3:+6373811 None:intergenic
TTTGTCTCAAATCAGGAAGT+GGG 0.580823 2_3:-6375797 Msa0274530:five_prime_UTR
ATGTTCACTGTGCTGAGGTG+TGG 0.588500 2_3:-6375074 Msa0274530:CDS
GTCTGCTCATGTTCAGAGTG+AGG 0.590066 2_3:-6375394 Msa0274530:CDS
AAGCAAAAGCTGATGAATCA+GGG 0.591275 2_3:-6375344 Msa0274530:CDS
ATGAAGTGTAGCAGCCACAA+AGG 0.594471 2_3:-6375252 Msa0274530:CDS
CAATTTGGTGAATCAAGAAG+TGG 0.596477 2_3:-6375439 Msa0274530:CDS
CTAATATAAGAGTGAATGAG+TGG 0.599350 2_3:-6373648 Msa0274530:three_prime_UTR
GTTTCGATGAAAGTGCCGAA+AGG 0.607405 2_3:-6375312 Msa0274530:CDS
TCTGCTCATGTTCAGAGTGA+GGG 0.612802 2_3:-6375393 Msa0274530:CDS
TGTGCGTGTAAAGACGAGCT+TGG 0.628343 2_3:-6375105 Msa0274530:CDS
TTCGATGAAAGTGCCGAAAG+GGG 0.628627 2_3:-6375310 Msa0274530:CDS
TGCACATGTTCACTGTGCTG+AGG 0.630611 2_3:-6375079 Msa0274530:CDS
GAGTGAGGGTGTTAATGGAG+AGG 0.641731 2_3:-6375379 Msa0274530:CDS
AGAAGTTGGAACTTCAAGAG+AGG 0.649974 2_3:-6375517 Msa0274530:CDS
AGTGGAGATCTAATTCAGCT+CGG 0.661685 2_3:-6375129 Msa0274530:CDS
GAAAATGCTAGTGCTGCAAG+TGG 0.663201 2_3:-6375147 Msa0274530:CDS
CACGCAAGATAAAACATGCG+TGG 0.669961 2_3:+6376001 None:intergenic
ACGACGATAACTCATCCCGC+AGG 0.676581 2_3:-6373827 Msa0274530:CDS
AACAGGTTATGTGAAATATG+CGG 0.695240 2_3:-6373927 Msa0274530:intron
AGGGTGTTAATGGAGAGGCG+AGG 0.702113 2_3:-6375374 Msa0274530:CDS
AAAAGGGTAATATAGGAGCG+GGG 0.727460 2_3:+6376071 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAAAAGTTTCATTTTTTA+AGG + chr2_3:6373653-6373672 None:intergenic 10.0%
!!! AAATTTCTTTTTTATTACAT+AGG + chr2_3:6373764-6373783 None:intergenic 10.0%
!!! GTAATAAAAAAGAAATTTTT+TGG - chr2_3:6373766-6373785 Msa0274530:CDS 10.0%
!!! TCTTTTTTTTAAAAAAAGTT+GGG - chr2_3:6375188-6375207 Msa0274530:CDS 10.0%
!!! TTGTTTTATGTAATTTTTGT+TGG - chr2_3:6373812-6373831 Msa0274530:CDS 15.0%
!!! TGTTTTATGTAATTTTTGTT+GGG - chr2_3:6373813-6373832 Msa0274530:CDS 15.0%
!! ATAAAGATATAATAGACAAA+TGG + chr2_3:6374668-6374687 None:intergenic 15.0%
!! TCTAATAACATTTAGTATTA+AGG + chr2_3:6374760-6374779 None:intergenic 15.0%
!!! GTCTTTTTTTTAAAAAAAGT+TGG - chr2_3:6375187-6375206 Msa0274530:CDS 15.0%
!!! CTTTTTTTTAAAAAAAGTTG+GGG - chr2_3:6375189-6375208 Msa0274530:CDS 15.0%
!!! ATTTTCTAATAATGTTTGTT+GGG + chr2_3:6375320-6375339 None:intergenic 15.0%
!!! AATGATCTTATTTGATAATA+TGG + chr2_3:6375515-6375534 None:intergenic 15.0%
!!! AATAATGGAAAGAAATAATT+TGG + chr2_3:6375959-6375978 None:intergenic 15.0%
!!! AATTATTTCTTTCCATTATT+TGG - chr2_3:6375959-6375978 Msa0274530:five_prime_UTR 15.0%
!! TTATTTAGTGACAAAAACAT+AGG + chr2_3:6373304-6373323 None:intergenic 20.0%
!! AAACAGTAAAGAATTAGAAA+AGG + chr2_3:6373368-6373387 None:intergenic 20.0%
!! AATATTATAGGTTACATACT+AGG - chr2_3:6373612-6373631 Msa0274530:three_prime_UTR 20.0%
!!! AAATTTTTTGGCTTGATTTT+TGG - chr2_3:6373778-6373797 Msa0274530:CDS 20.0%
!!! CATTTTCTAATAATGTTTGT+TGG + chr2_3:6375321-6375340 None:intergenic 20.0%
!! CAACAAACATTATTAGAAAA+TGG - chr2_3:6375319-6375338 Msa0274530:CDS 20.0%
!! TATACATATACTCCAAATAA+TGG + chr2_3:6375974-6375993 None:intergenic 20.0%
! TTGGTTAAAAAGGGTAATAT+AGG + chr2_3:6373422-6373441 None:intergenic 25.0%
!! TGATGATGATTGGTTAAAAA+GGG + chr2_3:6373431-6373450 None:intergenic 25.0%
!! ATGATGATGATTGGTTAAAA+AGG + chr2_3:6373432-6373451 None:intergenic 25.0%
!! AAATTGAGAATGATGATGAT+TGG + chr2_3:6373441-6373460 None:intergenic 25.0%
!! TTCATTGAATTCAACTTGTT+TGG - chr2_3:6373518-6373537 Msa0274530:three_prime_UTR 25.0%
!!! CTATAATATTGATGATGACA+AGG + chr2_3:6373602-6373621 None:intergenic 25.0%
! CTTGTCATCATCAATATTAT+AGG - chr2_3:6373600-6373619 Msa0274530:three_prime_UTR 25.0%
! TGATGAGAATTTGAGTAATT+TGG - chr2_3:6373930-6373949 Msa0274530:intron 25.0%
! AATTTGAGTAATTTGGTTGA+AGG - chr2_3:6373937-6373956 Msa0274530:intron 25.0%
! ATTTGAGTAATTTGGTTGAA+GGG - chr2_3:6373938-6373957 Msa0274530:intron 25.0%
! TAGTTGTAATTCAAGCAATT+TGG - chr2_3:6374029-6374048 Msa0274530:intron 25.0%
! TTAGCACTTCAAAAATAAGT+TGG + chr2_3:6374570-6374589 None:intergenic 25.0%
!! GTTATGTTACCTTGATAATT+TGG - chr2_3:6374688-6374707 Msa0274530:intron 25.0%
! CACAACTTCAACAATAATAT+TGG - chr2_3:6374717-6374736 Msa0274530:intron 25.0%
! GCTTGAATTTATCCTTAATT+TGG - chr2_3:6374892-6374911 Msa0274530:intron 25.0%
! ACCATTATCACTAATGAAAT+TGG - chr2_3:6374942-6374961 Msa0274530:intron 25.0%
! ACCAATTTCATTAGTGATAA+TGG + chr2_3:6374946-6374965 None:intergenic 25.0%
! TAATCCTACAACCATAAAAA+GGG + chr2_3:6375157-6375176 None:intergenic 25.0%
! TTAATCCTACAACCATAAAA+AGG + chr2_3:6375158-6375177 None:intergenic 25.0%
!! TTTTATGGTTGTAGGATTAA+TGG - chr2_3:6375158-6375177 Msa0274530:CDS 25.0%
!!! TTTTAAAAAAAGTTGGGGAT+TGG - chr2_3:6375194-6375213 Msa0274530:CDS 25.0%
! TTTAAAAAAAGTTGGGGATT+GGG - chr2_3:6375195-6375214 Msa0274530:CDS 25.0%
! GAGAATGTTTGCAAATAATA+TGG + chr2_3:6375449-6375468 None:intergenic 25.0%
!!! AGCTATCATTTTTTAACAAC+AGG - chr2_3:6375539-6375558 Msa0274530:CDS 25.0%
!!! GCCATTTTGTAATTTCTTAA+TGG - chr2_3:6375702-6375721 Msa0274530:five_prime_UTR 25.0%
! GCCATTAAGAAATTACAAAA+TGG + chr2_3:6375706-6375725 None:intergenic 25.0%
! ATATCTACATGAAAAAACCA+TGG + chr2_3:6375754-6375773 None:intergenic 25.0%
! TAGGTATGAAGAATTGAAAA+GGG + chr2_3:6375815-6375834 None:intergenic 25.0%
! TCATTCACTCTTATATTAGT+AGG + chr2_3:6375834-6375853 None:intergenic 25.0%
! AAATGACTACATAAGAAAGA+AGG + chr2_3:6376154-6376173 None:intergenic 25.0%
! TTCTTTCTTATGTAGTCATT+TGG - chr2_3:6376153-6376172 Msa0274530:five_prime_UTR 25.0%
TAAAAAGGGTAATATAGGAG+CGG + chr2_3:6373417-6373436 None:intergenic 30.0%
!!! TTTTTTGGCTTGATTTTTGG+TGG - chr2_3:6373781-6373800 Msa0274530:CDS 30.0%
!!! TTTTTGGCTTGATTTTTGGT+GGG - chr2_3:6373782-6373801 Msa0274530:CDS 30.0%
!!! TTTTTGTTGGGAGTTTGTTA+AGG - chr2_3:6373825-6373844 Msa0274530:CDS 30.0%
! AGAGTTGAAGGAATTTTCAA+TGG - chr2_3:6373852-6373871 Msa0274530:CDS 30.0%
! GAGTTGAAGGAATTTTCAAT+GGG - chr2_3:6373853-6373872 Msa0274530:CDS 30.0%
! TGAAGTTAGAGTTGAAGTTT+TGG - chr2_3:6373888-6373907 Msa0274530:CDS 30.0%
!!! TAGAGTTGAAGTTTTGGATT+CGG - chr2_3:6373894-6373913 Msa0274530:CDS 30.0%
TTTGAGTAATTTGGTTGAAG+GGG - chr2_3:6373939-6373958 Msa0274530:intron 30.0%
TAAGTGTGATGAATTGGTTA+TGG - chr2_3:6373993-6374012 Msa0274530:intron 30.0%
!!! TGAGATTGACATGTTTTTTG+AGG - chr2_3:6374501-6374520 Msa0274530:intron 30.0%
!!! TTTTTGAGGTTTTGCATTGA+CGG - chr2_3:6374515-6374534 Msa0274530:intron 30.0%
!!! TTTTGAAGTGCTAATGTTGT+TGG - chr2_3:6374576-6374595 Msa0274530:intron 30.0%
! TTTGAAGTGCTAATGTTGTT+GGG - chr2_3:6374577-6374596 Msa0274530:intron 30.0%
TATAATAGACAAATGGCAAG+AGG + chr2_3:6374661-6374680 None:intergenic 30.0%
GTGAATGTTCCAAATTATCA+AGG + chr2_3:6374700-6374719 None:intergenic 30.0%
TAGAACAAGAGTTAAGAAGT+AGG - chr2_3:6374781-6374800 Msa0274530:intron 30.0%
!! TGAATCTAGAAAGCATTTGA+AGG + chr2_3:6374859-6374878 None:intergenic 30.0%
ATGCTTTCTAGATTCATTGT+TGG - chr2_3:6374863-6374882 Msa0274530:intron 30.0%
TGCTTTCTAGATTCATTGTT+GGG - chr2_3:6374864-6374883 Msa0274530:intron 30.0%
!!! TAGTGAATGTAGTTTTATGC+AGG - chr2_3:6375095-6375114 Msa0274530:CDS 30.0%
!!! TAGTTTTATGCAGGAATAGT+CGG - chr2_3:6375104-6375123 Msa0274530:CDS 30.0%
AATCCTACAACCATAAAAAG+GGG + chr2_3:6375156-6375175 None:intergenic 30.0%
!! GTTGTAGGATTAATGGATTA+TGG - chr2_3:6375165-6375184 Msa0274530:CDS 30.0%
TTAAAAAAAGTTGGGGATTG+GGG - chr2_3:6375196-6375215 Msa0274530:CDS 30.0%
ATTGTAAGTTTGTAACCTTG+AGG + chr2_3:6375269-6375288 None:intergenic 30.0%
CATTATTAGAAAATGGCTTC+TGG - chr2_3:6375326-6375345 Msa0274530:CDS 30.0%
! ATGTCAAACTGGAATTTTCA+TGG + chr2_3:6375386-6375405 None:intergenic 30.0%
AAAATTGCTAGATGTCAAAC+TGG + chr2_3:6375397-6375416 None:intergenic 30.0%
TATTTGCAAACATTCTCTTG+TGG - chr2_3:6375452-6375471 Msa0274530:CDS 30.0%
! GAAGATTCTTTTGGATTATG+TGG - chr2_3:6375485-6375504 Msa0274530:CDS 30.0%
AACAGGTTATGTGAAATATG+CGG - chr2_3:6375556-6375575 Msa0274530:CDS 30.0%
TGAGACTGCAAAAAATGTTT+CGG - chr2_3:6375579-6375598 Msa0274530:CDS 30.0%
!! TGTTGCTAGTAATGGATTTA+TGG - chr2_3:6375603-6375622 Msa0274530:CDS 30.0%
!! ATGTAGATATGTTCTAGCTT+GGG - chr2_3:6375764-6375783 Msa0274530:five_prime_UTR 30.0%
GTAGGTATGAAGAATTGAAA+AGG + chr2_3:6375816-6375835 None:intergenic 30.0%
CTAATATAAGAGTGAATGAG+TGG - chr2_3:6375835-6375854 Msa0274530:five_prime_UTR 30.0%
! GGAGTATATGTATAAGTTGA+TGG - chr2_3:6375980-6375999 Msa0274530:five_prime_UTR 30.0%
AAAAAGGGTAATATAGGAGC+GGG + chr2_3:6373416-6373435 None:intergenic 35.0%
AAGATAAAACATGCGTGGTT+TGG + chr2_3:6373480-6373499 None:intergenic 35.0%
GAAACATATGCAGAGAGAAA+GGG + chr2_3:6373567-6373586 None:intergenic 35.0%
TGAAACATATGCAGAGAGAA+AGG + chr2_3:6373568-6373587 None:intergenic 35.0%
TTTCTCTCTGCATATGTTTC+AGG - chr2_3:6373567-6373586 Msa0274530:three_prime_UTR 35.0%
! CTGAGATTTTGTCTCAAATC+AGG - chr2_3:6373679-6373698 Msa0274530:three_prime_UTR 35.0%
! TTTTGTCTCAAATCAGGAAG+TGG - chr2_3:6373685-6373704 Msa0274530:three_prime_UTR 35.0%
TTTGTCTCAAATCAGGAAGT+GGG - chr2_3:6373686-6373705 Msa0274530:three_prime_UTR 35.0%
!!! TTTTGGCTTGATTTTTGGTG+GGG - chr2_3:6373783-6373802 Msa0274530:CDS 35.0%
TGTTAAGGTTTGAGAGTTGA+AGG - chr2_3:6373840-6373859 Msa0274530:CDS 35.0%
!! GGTTGATAAGTGTGATGAAT+TGG - chr2_3:6373987-6374006 Msa0274530:intron 35.0%
GAATTGGTTATGGTTGATCA+AGG - chr2_3:6374003-6374022 Msa0274530:intron 35.0%
! CAATTTGGTGAATCAAGAAG+TGG - chr2_3:6374044-6374063 Msa0274530:intron 35.0%
AAGCAAAAGCTGATGAATCA+GGG - chr2_3:6374139-6374158 Msa0274530:intron 35.0%
! TGCATTGACGGTTTCAATAT+TGG - chr2_3:6374527-6374546 Msa0274530:intron 35.0%
TTAGAAATGCTCTCAACTGA+AGG + chr2_3:6374834-6374853 None:intergenic 35.0%
AGAAGCTGAACTCCAAATTA+AGG + chr2_3:6374907-6374926 None:intergenic 35.0%
! TTAGTGTTGCTCAATCTTAC+TGG - chr2_3:6374965-6374984 Msa0274530:intron 35.0%
TAAAAAAAGTTGGGGATTGG+GGG - chr2_3:6375197-6375216 Msa0274530:CDS 35.0%
TAAGTTTGTAACCTTGAGGA+CGG + chr2_3:6375265-6375284 None:intergenic 35.0%
AATCCAAAAGAATCTTCCTC+AGG + chr2_3:6375482-6375501 None:intergenic 35.0%
GAGACTGCAAAAAATGTTTC+GGG - chr2_3:6375580-6375599 Msa0274530:CDS 35.0%
!! GTAATGGATTTATGGAAGAG+TGG - chr2_3:6375611-6375630 Msa0274530:CDS 35.0%
GTGGAATGATAGAAGATTCA+TGG - chr2_3:6375630-6375649 Msa0274530:exon 35.0%
! CATGTAGATATGTTCTAGCT+TGG - chr2_3:6375763-6375782 Msa0274530:five_prime_UTR 35.0%
CAGCGAATTTCAAATCTTCT+CGG + chr2_3:6375897-6375916 None:intergenic 35.0%
AAAAGGGTAATATAGGAGCG+GGG + chr2_3:6373415-6373434 None:intergenic 40.0%
! ATGTTTTATCTTGCGTGGCT+TGG - chr2_3:6373488-6373507 Msa0274530:three_prime_UTR 40.0%
AGTAATTTGGTTGAAGGGGT+TGG - chr2_3:6373943-6373962 Msa0274530:intron 40.0%
AGAAGTTGGAACTTCAAGAG+AGG - chr2_3:6373966-6373985 Msa0274530:intron 40.0%
GAAGCAAAAGCTGATGAATC+AGG - chr2_3:6374138-6374157 Msa0274530:intron 40.0%
TGATGAATCAGGGCTTAGTT+TGG - chr2_3:6374149-6374168 Msa0274530:intron 40.0%
!! TTTCGATGAAAGTGCCGAAA+GGG - chr2_3:6374172-6374191 Msa0274530:intron 40.0%
AGCTCTCATAAAACCCTTTG+TGG + chr2_3:6374248-6374267 None:intergenic 40.0%
GGTTTGTAGGATTTGTCATC+TGG - chr2_3:6374281-6374300 Msa0274530:intron 40.0%
!!! AGGATTTGTCATCTGGCTTT+TGG - chr2_3:6374288-6374307 Msa0274530:intron 40.0%
!!! GGATTTGTCATCTGGCTTTT+GGG - chr2_3:6374289-6374308 Msa0274530:intron 40.0%
AGTGGAGATCTAATTCAGCT+CGG - chr2_3:6374354-6374373 Msa0274530:intron 40.0%
GCTTAAGGGAAACAGGTATA+TGG - chr2_3:6374437-6374456 Msa0274530:intron 40.0%
AATGCTCTCAACTGAAGGAT+AGG + chr2_3:6374829-6374848 None:intergenic 40.0%
!! TTGCTCAATCTTACTGGCTT+TGG - chr2_3:6374971-6374990 Msa0274530:intron 40.0%
TGCAAGTTACAACTACGTCT+AGG + chr2_3:6375043-6375062 None:intergenic 40.0%
ATGTATCCAGACACCCAATA+TGG - chr2_3:6375130-6375149 Msa0274530:CDS 40.0%
CCATAAAAAGGGGCCATATT+GGG + chr2_3:6375146-6375165 None:intergenic 40.0%
ACCATAAAAAGGGGCCATAT+TGG + chr2_3:6375147-6375166 None:intergenic 40.0%
! TGTCCTGAGGAAGATTCTTT+TGG - chr2_3:6375476-6375495 Msa0274530:CDS 40.0%
TAATTTCTTAATGGCGTGCC+TGG - chr2_3:6375711-6375730 Msa0274530:five_prime_UTR 40.0%
TAATAGCGTTCATTCTGCCA+TGG - chr2_3:6375734-6375753 Msa0274530:five_prime_UTR 40.0%
GAAGTTACGAGCTTGATAAG+TGG - chr2_3:6375929-6375948 Msa0274530:five_prime_UTR 40.0%
CACGCAAGATAAAACATGCG+TGG + chr2_3:6373485-6373504 None:intergenic 45.0%
! CACGCATGTTTTATCTTGCG+TGG - chr2_3:6373483-6373502 Msa0274530:three_prime_UTR 45.0%
TCTGCTCATGTTCAGAGTGA+GGG - chr2_3:6374090-6374109 Msa0274530:intron 45.0%
!! GTTCAGAGTGAGGGTGTTAA+TGG - chr2_3:6374099-6374118 Msa0274530:intron 45.0%
!! GTTTCGATGAAAGTGCCGAA+AGG - chr2_3:6374171-6374190 Msa0274530:intron 45.0%
!! TTCGATGAAAGTGCCGAAAG+GGG - chr2_3:6374173-6374192 Msa0274530:intron 45.0%
TCAGTTTCAGACACCCCTTT+CGG + chr2_3:6374189-6374208 None:intergenic 45.0%
ATGAAGTGTAGCAGCCACAA+AGG - chr2_3:6374231-6374250 Msa0274530:intron 45.0%
TGAAGTGTAGCAGCCACAAA+GGG - chr2_3:6374232-6374251 Msa0274530:intron 45.0%
!! GGGTTTTATGAGAGCTCACA+AGG - chr2_3:6374252-6374271 Msa0274530:intron 45.0%
ATGAGAGCTCACAAGGTGAA+AGG - chr2_3:6374259-6374278 Msa0274530:intron 45.0%
TGAGAGCTCACAAGGTGAAA+GGG - chr2_3:6374260-6374279 Msa0274530:intron 45.0%
CACAAGGTGAAAGGGTTTGT+AGG - chr2_3:6374268-6374287 Msa0274530:intron 45.0%
GAAAATGCTAGTGCTGCAAG+TGG - chr2_3:6374336-6374355 Msa0274530:intron 45.0%
TGAGGTGTGGTTCAAGCTTA+AGG - chr2_3:6374422-6374441 Msa0274530:intron 45.0%
GAGGTGTGGTTCAAGCTTAA+GGG - chr2_3:6374423-6374442 Msa0274530:intron 45.0%
GGTTCAAGCTTAAGGGAAAC+AGG - chr2_3:6374430-6374449 Msa0274530:intron 45.0%
!! CAATCTTACTGGCTTTGGAC+TGG - chr2_3:6374976-6374995 Msa0274530:intron 45.0%
! CCCAATATGGCCCCTTTTTA+TGG - chr2_3:6375143-6375162 Msa0274530:CDS 45.0%
! TGGCCCCTTTTTATGGTTGT+AGG - chr2_3:6375150-6375169 Msa0274530:CDS 45.0%
! TCATTTTGCAACCGTCCTCA+AGG - chr2_3:6375251-6375270 Msa0274530:CDS 45.0%
ATTCTCTTGTGGTTGTCCTG+AGG - chr2_3:6375463-6375482 Msa0274530:CDS 45.0%
!! GTTTCGGGTGTTGCTAGTAA+TGG - chr2_3:6375595-6375614 Msa0274530:CDS 45.0%
GCAGAATGAACGCTATTACC+AGG + chr2_3:6375732-6375751 None:intergenic 45.0%
GGTAATATAGGAGCGGGGTT+CGG + chr2_3:6373410-6373429 None:intergenic 50.0%
GTTGAAGGGGTTGGAGAAGT+TGG - chr2_3:6373952-6373971 Msa0274530:intron 50.0%
GTCTGCTCATGTTCAGAGTG+AGG - chr2_3:6374089-6374108 Msa0274530:intron 50.0%
!! GAGTGAGGGTGTTAATGGAG+AGG - chr2_3:6374104-6374123 Msa0274530:intron 50.0%
!!! TTGTCATCTGGCTTTTGGGC+AGG - chr2_3:6374293-6374312 Msa0274530:intron 50.0%
TGTGCGTGTAAAGACGAGCT+TGG - chr2_3:6374378-6374397 Msa0274530:intron 50.0%
TGCACATGTTCACTGTGCTG+AGG - chr2_3:6374404-6374423 Msa0274530:intron 50.0%
ATGTTCACTGTGCTGAGGTG+TGG - chr2_3:6374409-6374428 Msa0274530:intron 50.0%
!! CTTACTGGCTTTGGACTGGT+AGG - chr2_3:6374980-6374999 Msa0274530:intron 50.0%
GATAACTCATCCCGCAGGTT+TGG - chr2_3:6375661-6375680 Msa0274530:five_prime_UTR 50.0%
! AGGGTGTTAATGGAGAGGCG+AGG - chr2_3:6374109-6374128 Msa0274530:intron 55.0%
AAGGGGCCATATTGGGTGTC+TGG + chr2_3:6375139-6375158 None:intergenic 55.0%
ACGACGATAACTCATCCCGC+AGG - chr2_3:6375656-6375675 Msa0274530:five_prime_UTR 55.0%
AACTCATCCCGCAGGTTTGG+TGG - chr2_3:6375664-6375683 Msa0274530:five_prime_UTR 55.0%
CCAGCATCCACCAAACCTGC+GGG + chr2_3:6375674-6375693 None:intergenic 60.0%
GCCAGCATCCACCAAACCTG+CGG + chr2_3:6375675-6375694 None:intergenic 60.0%
!! AGGTTTGGTGGATGCTGGCG+AGG - chr2_3:6375676-6375695 Msa0274530:five_prime_UTR 60.0%
!! CCCGCAGGTTTGGTGGATGC+TGG - chr2_3:6375671-6375690 Msa0274530:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 6373294 6376211 6373294 ID=Msa0274530;Name=Msa0274530
chr2_3 mRNA 6373294 6376211 6373294 ID=Msa0274530-mRNA-1;Parent=Msa0274530;Name=Msa0274530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=577|1|1|1|1|1|2|431|266
chr2_3 exon 6373294 6373944 6373294 ID=Msa0274530-mRNA-1:exon:2959;Parent=Msa0274530-mRNA-1
chr2_3 exon 6375054 6376211 6375054 ID=Msa0274530-mRNA-1:exon:2958;Parent=Msa0274530-mRNA-1
chr2_3 five_prime_UTR 6375635 6376211 6375635 ID=Msa0274530-mRNA-1:five_prime_utr;Parent=Msa0274530-mRNA-1
chr2_3 CDS 6375054 6375634 6375054 ID=Msa0274530-mRNA-1:cds;Parent=Msa0274530-mRNA-1
chr2_3 CDS 6373725 6373944 6373725 ID=Msa0274530-mRNA-1:cds;Parent=Msa0274530-mRNA-1
chr2_3 three_prime_UTR 6373294 6373724 6373294 ID=Msa0274530-mRNA-1:three_prime_utr;Parent=Msa0274530-mRNA-1
Gene Sequence

>Msa0274530

ATGGGTCTAGATGAGAGTGAAGTTAGAGTTGAAGTTTTGGATTCGGTTGATGAAGTGATTGATGAGAATTTGAGTAATTTGGTTGAAGGGGTTGGAGAAGTTGGAACTTCAAGAGAGGTTGATAAGTGTGATGAATTGGTTATGGTTGATCAAGGAACTAGTTGTAATTCAAGCAATTTGGTGAATCAAGAAGTGGTTGAGAGAGTGATTGTAATAGAGTCTGCTCATGTTCAGAGTGAGGGTGTTAATGGAGAGGCGAGGAAATTAGAAGCAAAAGCTGATGAATCAGGGCTTAGTTTGGTTTCGATGAAAGTGCCGAAAGGGGTGTCTGAAACTGAGAAAGATTCATGTGTGATTGATATGAAGTGTAGCAGCCACAAAGGGTTTTATGAGAGCTCACAAGGTGAAAGGGTTTGTAGGATTTGTCATCTGGCTTTTGGGCAGGCATCAGATGCAACAGCTGTTGAAAATGCTAGTGCTGCAAGTGGAGATCTAATTCAGCTCGGTTGTGCGTGTAAAGACGAGCTTGGCATTGCACATGTTCACTGTGCTGAGGTGTGGTTCAAGCTTAAGGGAAACAGGTTATGTGAAATATGCGGTGAGACTGCAAAAAATGTTTCGGGTGTTGCTAGTAATGGATTTATGGAAGAGTGGAATGATAGAAGATTCATGGATAACGACGATAACTCATCCCGCAGGTTTGGTGGATGCTGGCGAGGACAGCCATTTTGTAATTTCTTAATGGCGTGCCTGGTAATAGCGTTCATTCTGCCATGGTTTTTTCATGTAGATATGTTCTAG
Protein sequence

>Msa0274530

MGLDESEVRVEVLDSVDEVIDENLSNLVEGVGEVGTSREVDKCDELVMVDQGTSCNSSNLVNQEVVERVIVIESAHVQSEGVNGEARKLEAKADESGLSLVSMKVPKGVSETEKDSCVIDMKCSSHKGFYESSQGERVCRICHLAFGQASDATAVENASAASGDLIQLGCACKDELGIAHVHCAEVWFKLKGNRLCEICGETAKNVSGVASNGFMEEWNDRRFMDNDDNSSRRFGGCWRGQPFCNFLMACLVIAFILPWFFHVDMF*