AlfalfaGEDB | Msa0274530

M. sativa cultivar zhongmu-4 / Msa0274530

KEGG: -
GO: -
PFAM: PF12906.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274530	XP_013462590.1	90.106	283	11	1	1	266	1	283	0.0	509

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274530	A0A072VEM0	90.106	283	11	1	1	266	1	283	0.0	509

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0172660	Msa0274530	0.802731	4.880758e-49	-8.615850e-47
Msa0181740	Msa0274530	0.806252	9.004212e-50	-8.615850e-47
Msa0186750	Msa0274530	0.812412	4.294529e-51	-8.615850e-47
Msa0228320	Msa0274530	0.809006	2.341700e-50	-8.615850e-47
Msa0274530	Msa0274690	0.822259	2.606318e-53	-8.615850e-47
Msa0274530	Msa0307490	0.815836	7.536911e-52	-8.615850e-47
Msa0274530	Msa0322480	0.805431	1.339223e-49	-8.615850e-47
Msa0274530	Msa0417000	0.803642	3.162297e-49	-8.615850e-47
Msa0274530	Msa0439940	0.821004	5.084638e-53	-8.615850e-47
Msa0274530	Msa0482020	0.814307	1.647005e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274530	MtrunA17_Chr2g0284111	90.106	283	11	1	1	266	1	283	0.0	509
Msa0274530	MtrunA17_Chr4g0045171	54.305	302	88	6	12	266	1	299	9.07e-102	298
Msa0274530	MtrunA17_Chr8g0379191	54.098	305	86	6	11	266	11	310	2.18e-100	295
Msa0274530	MtrunA17_Chr5g0436201	42.336	137	60	4	136	265	85	209	1.67e-29	110
Msa0274530	MtrunA17_Chr3g0112791	41.481	135	59	3	136	261	88	211	3.31e-29	110
Msa0274530	MtrunA17_Chr7g0240961	38.732	142	58	4	136	265	108	232	1.54e-26	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274530	AT5G05830.1	40.000	155	73	4	119	265	59	201	3.70e-31	115
Msa0274530	AT2G34200.2	46.457	127	56	4	135	261	98	212	2.38e-28	108
Msa0274530	AT2G34200.1	46.457	127	56	4	135	261	98	212	2.38e-28	108
Msa0274530	AT5G01070.1	38.571	140	64	4	136	265	75	202	2.30e-25	100
Msa0274530	AT5G59000.1	43.269	104	59	0	163	266	127	230	1.47e-24	98.6
Msa0274530	AT2G37950.1	37.037	135	62	4	136	262	81	200	2.28e-24	97.4

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Find 87 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGTAATATAGGAGCG+GGG	0.727460	2_3:+6376071	None:intergenic
AAACAGTAAAGAATTAGAAA+AGG	0.496102	2_3:+6376118	None:intergenic
AAATGACTACATAAGAAAGA+AGG	0.564888	2_3:+6373332	None:intergenic
AAATTGAGAATGATGATGAT+TGG	0.500097	2_3:+6376045	None:intergenic
AACAGGTTATGTGAAATATG+CGG	0.695240	2_3:-6373927	Msa0274530:intron
AACTCATCCCGCAGGTTTGG+TGG	0.479462	2_3:-6373819	Msa0274530:CDS
AAGATAAAACATGCGTGGTT+TGG	0.403032	2_3:+6376006	None:intergenic
AAGCAAAAGCTGATGAATCA+GGG	0.591275	2_3:-6375344	Msa0274530:CDS
AATAATGGAAAGAAATAATT+TGG	0.250141	2_3:+6373527	None:intergenic
AATATTATAGGTTACATACT+AGG	0.448065	2_3:-6375871	Msa0274530:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAAAAGGGTAATATAGGAG+CGG	+	chr2_3:6373417-6373436	None:intergenic	30.0%
TTTGAGTAATTTGGTTGAAG+GGG	-	chr2_3:6373939-6373958	Msa0274530:intron	30.0%
TAAGTGTGATGAATTGGTTA+TGG	-	chr2_3:6373993-6374012	Msa0274530:intron	30.0%
TATAATAGACAAATGGCAAG+AGG	+	chr2_3:6374661-6374680	None:intergenic	30.0%
GTGAATGTTCCAAATTATCA+AGG	+	chr2_3:6374700-6374719	None:intergenic	30.0%
TAGAACAAGAGTTAAGAAGT+AGG	-	chr2_3:6374781-6374800	Msa0274530:intron	30.0%
ATGCTTTCTAGATTCATTGT+TGG	-	chr2_3:6374863-6374882	Msa0274530:intron	30.0%
TGCTTTCTAGATTCATTGTT+GGG	-	chr2_3:6374864-6374883	Msa0274530:intron	30.0%
AATCCTACAACCATAAAAAG+GGG	+	chr2_3:6375156-6375175	None:intergenic	30.0%
TTAAAAAAAGTTGGGGATTG+GGG	-	chr2_3:6375196-6375215	Msa0274530:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	6373294	6376211	6373294	ID=Msa0274530;Name=Msa0274530
chr2_3	mRNA	6373294	6376211	6373294	ID=Msa0274530-mRNA-1;Parent=Msa0274530;Name=Msa0274530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=577\|1\|1\|1\|1\|1\|2\|431\|266
chr2_3	exon	6373294	6373944	6373294	ID=Msa0274530-mRNA-1:exon:2959;Parent=Msa0274530-mRNA-1
chr2_3	exon	6375054	6376211	6375054	ID=Msa0274530-mRNA-1:exon:2958;Parent=Msa0274530-mRNA-1
chr2_3	five_prime_UTR	6375635	6376211	6375635	ID=Msa0274530-mRNA-1:five_prime_utr;Parent=Msa0274530-mRNA-1
chr2_3	CDS	6375054	6375634	6375054	ID=Msa0274530-mRNA-1:cds;Parent=Msa0274530-mRNA-1
chr2_3	CDS	6373725	6373944	6373725	ID=Msa0274530-mRNA-1:cds;Parent=Msa0274530-mRNA-1
chr2_3	three_prime_UTR	6373294	6373724	6373294	ID=Msa0274530-mRNA-1:three_prime_utr;Parent=Msa0274530-mRNA-1

Gene Sequence

>Msa0274530

ATGGGTCTAGATGAGAGTGAAGTTAGAGTTGAAGTTTTGGATTCGGTTGATGAAGTGATTGATGAGAATTTGAGTAATTTGGTTGAAGGGGTTGGAGAAGTTGGAACTTCAAGAGAGGTTGATAAGTGTGATGAATTGGTTATGGTTGATCAAGGAACTAGTTGTAATTCAAGCAATTTGGTGAATCAAGAAGTGGTTGAGAGAGTGATTGTAATAGAGTCTGCTCATGTTCAGAGTGAGGGTGTTAATGGAGAGGCGAGGAAATTAGAAGCAAAAGCTGATGAATCAGGGCTTAGTTTGGTTTCGATGAAAGTGCCGAAAGGGGTGTCTGAAACTGAGAAAGATTCATGTGTGATTGATATGAAGTGTAGCAGCCACAAAGGGTTTTATGAGAGCTCACAAGGTGAAAGGGTTTGTAGGATTTGTCATCTGGCTTTTGGGCAGGCATCAGATGCAACAGCTGTTGAAAATGCTAGTGCTGCAAGTGGAGATCTAATTCAGCTCGGTTGTGCGTGTAAAGACGAGCTTGGCATTGCACATGTTCACTGTGCTGAGGTGTGGTTCAAGCTTAAGGGAAACAGGTTATGTGAAATATGCGGTGAGACTGCAAAAAATGTTTCGGGTGTTGCTAGTAATGGATTTATGGAAGAGTGGAATGATAGAAGATTCATGGATAACGACGATAACTCATCCCGCAGGTTTGGTGGATGCTGGCGAGGACAGCCATTTTGTAATTTCTTAATGGCGTGCCTGGTAATAGCGTTCATTCTGCCATGGTTTTTTCATGTAGATATGTTCTAG

Protein sequence

>Msa0274530

MGLDESEVRVEVLDSVDEVIDENLSNLVEGVGEVGTSREVDKCDELVMVDQGTSCNSSNLVNQEVVERVIVIESAHVQSEGVNGEARKLEAKADESGLSLVSMKVPKGVSETEKDSCVIDMKCSSHKGFYESSQGERVCRICHLAFGQASDATAVENASAASGDLIQLGCACKDELGIAHVHCAEVWFKLKGNRLCEICGETAKNVSGVASNGFMEEWNDRRFMDNDDNSSRRFGGCWRGQPFCNFLMACLVIAFILPWFFHVDMF*