AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0283390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0283390 MtrunA17_Chr1g0155331 97.951 244 5 0 1 244 1 244 1.78e-178 489
Msa0283390 MtrunA17_Chr2g0298841 98.361 244 4 0 1 244 1 244 3.01e-178 489
Msa0283390 MtrunA17_Chr4g0002321 86.722 241 32 0 4 244 3 243 6.72e-159 440
Msa0283390 MtrunA17_Chr7g0237701 45.749 247 130 2 1 244 1 246 1.94e-73 223
Msa0283390 MtrunA17_Chr1g0155311 98.361 61 1 0 174 234 2 62 3.29e-39 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0283390 AT3G13580.5 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0283390 AT3G13580.4 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0283390 AT3G13580.2 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0283390 AT3G13580.3 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0283390 AT3G13580.1 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0283390 AT3G13580.7 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0283390 AT3G13580.8 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0283390 AT3G13580.6 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0283390 AT3G13580.9 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0283390 AT2G01250.1 84.583 240 37 0 5 244 3 242 6.89e-152 422
Msa0283390 AT2G44120.2 83.333 240 40 0 5 244 8 247 2.47e-150 419
Msa0283390 AT2G44120.1 83.333 240 40 0 5 244 3 242 2.86e-150 418
Msa0283390 AT2G01250.2 80.000 175 35 0 5 179 3 177 5.40e-97 281
Msa0283390 AT1G80750.1 43.320 247 137 2 1 244 1 247 1.95e-63 198

Find 83 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAAATCTTCCTTGATTT+TGG 0.006132 2_3:+20544030 None:intergenic
TTCTCTGACACTCTTCAAAT+TGG 0.182246 2_3:+20542968 None:intergenic
TGCTTCTTCAAAACAGATTC+AGG 0.223629 2_3:+20544697 None:intergenic
AATATTCAATTGCAGGTTCT+TGG 0.263781 2_3:-20542572 Msa0283390:intron
ACTCATTAGAAGGATGAATT+AGG 0.270598 2_3:-20542363 Msa0283390:exon
TACAATTGTTAGAATCTATA+TGG 0.271097 2_3:-20542113 Msa0283390:three_prime_UTR
GTGTCGTTTAAGGTTGATTA+TGG 0.274625 2_3:-20542331 Msa0283390:three_prime_UTR
CTGAAACCAGGAAGCTTATC+TGG 0.274950 2_3:-20544609 Msa0283390:CDS
AAGGGAGGAGTAATTTATTA+AGG 0.279065 2_3:-20541954 Msa0283390:three_prime_UTR
TAGAAGGATGAATTAGGTTT+TGG 0.280297 2_3:-20542357 Msa0283390:exon
TTTCAAGCTCAAAGCTCCTT+TGG 0.284641 2_3:-20542459 Msa0283390:CDS
GTGGTTTCTTAACTAAAATA+AGG 0.289087 2_3:-20542018 Msa0283390:three_prime_UTR
TTCAAGCTCAAAGCTCCTTT+GGG 0.329811 2_3:-20542458 Msa0283390:CDS
TTGATCTACAAGCGTGGTTA+TGG 0.334090 2_3:-20542945 Msa0283390:CDS
TAAAAGTAGTATGTTTGTAT+TGG 0.337358 2_3:-20541927 Msa0283390:three_prime_UTR
TTCAGCAAAAGGAGTTGATT+AGG 0.371855 2_3:-20544456 Msa0283390:intron
AAAACTTTCACTGCACTTGC+AGG 0.373710 2_3:+20542210 None:intergenic
TCTACTCCAGATAAGCTTCC+TGG 0.375959 2_3:+20544603 None:intergenic
AACACTTGAACTCAACATAA+AGG 0.387036 2_3:-20541973 Msa0283390:three_prime_UTR
AAGGTGGTGATGCTGGTAAC+AGG 0.401808 2_3:-20542403 Msa0283390:CDS
ATATTCAATTGCAGGTTCTT+GGG 0.423006 2_3:-20542571 Msa0283390:intron
GTATTCCTTAGCGTACTGTT+TGG 0.423633 2_3:+20544579 None:intergenic
ACACTTGAACTCAACATAAA+GGG 0.426348 2_3:-20541972 Msa0283390:three_prime_UTR
TGGTTTCTTAACTAAAATAA+GGG 0.436696 2_3:-20542017 Msa0283390:three_prime_UTR
ATGGGCATTGGTGAAGAAAC+AGG 0.439903 2_3:-20544659 Msa0283390:CDS
ACAACATCGATTCACTGAAA+TGG 0.441842 2_3:-20544746 Msa0283390:exon
ACATCAATGAACTCATTAGA+AGG 0.442744 2_3:-20542373 Msa0283390:CDS
TAATTTCAGTATCAATGCTA+TGG 0.445420 2_3:-20544055 Msa0283390:intron
CTTATTTGTCAATGATGACT+TGG 0.447185 2_3:-20542185 Msa0283390:three_prime_UTR
TGAAGGTGAACGGCGCTTCA+AGG 0.448474 2_3:+20544787 None:intergenic
GCAGCTTCTGCGATTGAGAC+AGG 0.461345 2_3:-20544010 Msa0283390:intron
GTTGAGCCATATGTTACCTA+TGG 0.461514 2_3:-20543085 Msa0283390:CDS
TCTCTGACACTCTTCAAATT+GGG 0.464078 2_3:+20542969 None:intergenic
GAAGCGTGAAGCTAAGCTTA+AGG 0.464202 2_3:-20544431 Msa0283390:CDS
AAGCGTGAAGCTAAGCTTAA+GGG 0.467088 2_3:-20544430 Msa0283390:CDS
ATGAACAAAAGCTTAGCTTC+AGG 0.472114 2_3:+20544391 None:intergenic
AGCAGAAAAGGAACGAGGAA+TGG 0.472482 2_3:-20544678 Msa0283390:CDS
GCATACCCATAGGTAACATA+TGG 0.476296 2_3:+20543079 None:intergenic
AGATAACTCTATCATCGAAC+AGG 0.483740 2_3:-20542892 Msa0283390:intron
AAGAGAAATCACTATGTTGA+AGG 0.489111 2_3:-20542422 Msa0283390:CDS
CAACAGTGAATATGCTCCAC+AGG 0.489617 2_3:-20543108 Msa0283390:CDS
AGAAGAAGAGAGCTGAAACC+AGG 0.494410 2_3:-20544621 Msa0283390:CDS
AAAGGAGCTTTGAGCTTGAA+AGG 0.504831 2_3:+20542461 None:intergenic
GAGAGGCAAACAACTTCCTA+TGG 0.504947 2_3:-20542484 Msa0283390:CDS
TCTCTGAGTGAAAGTGAAGC+AGG 0.506218 2_3:-20542088 Msa0283390:three_prime_UTR
TTCTGTGTTAATATTTGTGA+TGG 0.508992 2_3:-20542048 Msa0283390:three_prime_UTR
TATTTGTGATGGGAGAATAG+TGG 0.511227 2_3:-20542037 Msa0283390:three_prime_UTR
GCAGAAAAGGAACGAGGAAT+GGG 0.522675 2_3:-20544677 Msa0283390:CDS
TTCTGTTCTGTGAAGGTGAA+CGG 0.523516 2_3:+20544777 None:intergenic
TGAAGTGAGGTCCAACAGTA+AGG 0.528046 2_3:+20542507 None:intergenic
ATGCTATGGACCCAAAATCA+AGG 0.531480 2_3:-20544041 Msa0283390:CDS
TATGTTGAAGGTGGTGATGC+TGG 0.533299 2_3:-20542410 Msa0283390:CDS
GTTGTAGTTCTGTTCTGTGA+AGG 0.539145 2_3:+20544770 None:intergenic
ATTCATGAGATCCTTACTGT+TGG 0.542331 2_3:-20542518 Msa0283390:CDS
GTGGTTATGGAAAGGTTGAT+AGG 0.547437 2_3:-20542932 Msa0283390:CDS
TTGCAGGTTCTTGGGAAACA+TGG 0.547799 2_3:-20542563 Msa0283390:intron
TTCTTCTTCATGCCACCCAA+AGG 0.554983 2_3:+20542443 None:intergenic
TTGTTGTTTGCATTCACGAA+AGG 0.561345 2_3:-20542149 Msa0283390:three_prime_UTR
AAAGTGAAGCAGGGAATGTA+CGG 0.567822 2_3:-20542078 Msa0283390:three_prime_UTR
CTTTGAGCTTGAAAGGCCAT+AGG 0.568033 2_3:+20542468 None:intergenic
TTGTTCATTATCAGGATCAG+AGG 0.568101 2_3:-20544376 Msa0283390:CDS
CGCTAAGGAATACGATGACT+CGG 0.569371 2_3:-20544569 Msa0283390:intron
AAGCTCAAAGCTCCTTTGGG+TGG 0.570830 2_3:-20542455 Msa0283390:CDS
TTGTTTGCCTCTCTGAAGTG+AGG 0.573961 2_3:+20542494 None:intergenic
AAGGAACGAGGAATGGGCAT+TGG 0.574615 2_3:-20544671 Msa0283390:CDS
CAAAACAGATTCAGGAACAA+CGG 0.575093 2_3:+20544705 None:intergenic
TGTTCATTATCAGGATCAGA+GGG 0.584717 2_3:-20544375 Msa0283390:intron
TTGAGCCATATGTTACCTAT+GGG 0.589704 2_3:-20543084 Msa0283390:intron
TCTGTGTTAATATTTGTGAT+GGG 0.595084 2_3:-20542047 Msa0283390:three_prime_UTR
ATTCACTGAAATGGCCAAAG+AGG 0.608425 2_3:-20544737 Msa0283390:exon
CTCTGAGTGAAAGTGAAGCA+GGG 0.610321 2_3:-20542087 Msa0283390:three_prime_UTR
CTACAAGCGTGGTTATGGAA+AGG 0.617075 2_3:-20542940 Msa0283390:CDS
TAGAGCCAAACAGTACGCTA+AGG 0.631717 2_3:-20544584 Msa0283390:CDS
CGTGAAGCTAAGCTTAAGGG+TGG 0.634387 2_3:-20544427 Msa0283390:CDS
AGAAATCACTATGTTGAAGG+TGG 0.637998 2_3:-20542419 Msa0283390:CDS
AGGTGGTGATGCTGGTAACA+GGG 0.651466 2_3:-20542402 Msa0283390:CDS
CTTGAACTCAACATAAAGGG+AGG 0.665267 2_3:-20541969 Msa0283390:three_prime_UTR
TAACATATGGCTCAACCCTG+TGG 0.667309 2_3:+20543092 None:intergenic
AACAGTGAATATGCTCCACA+GGG 0.679027 2_3:-20543107 Msa0283390:CDS
GAAGAAGCAGAAAAGGAACG+AGG 0.689511 2_3:-20544683 Msa0283390:CDS
AGAGAATTGATCTACAAGCG+TGG 0.711706 2_3:-20542951 Msa0283390:CDS
TGTTGGACCTCACTTCAGAG+AGG 0.727556 2_3:-20542501 Msa0283390:CDS
TGGTGATGCTGGTAACAGGG+AGG 0.759776 2_3:-20542399 Msa0283390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTATTATTTATTAA+TGG - chr2_3:20542597-20542616 Msa0283390:intron 0.0%
!! TATTATTATTATTTATTAAT+GGG - chr2_3:20542598-20542617 Msa0283390:intron 0.0%
!!! TTTTACAACATGTTTTATTT+CGG + chr2_3:20542411-20542430 None:intergenic 15.0%
!! AAATTGAATACAATTTATGT+AGG - chr2_3:20542506-20542525 Msa0283390:CDS 15.0%
!!! AATTCAGTTTTTTTTTGAAT+TGG - chr2_3:20542709-20542728 Msa0283390:intron 15.0%
!!! AAGCAAAAACAATTTTTTTT+GGG + chr2_3:20543093-20543112 None:intergenic 15.0%
!!! TAAGCAAAAACAATTTTTTT+TGG + chr2_3:20543094-20543113 None:intergenic 15.0%
!! TCATCTATATAATATAAGAA+TGG - chr2_3:20543133-20543152 Msa0283390:CDS 15.0%
!!! TTTTTTTTAGCTTAGATATA+AGG - chr2_3:20543386-20543405 Msa0283390:intron 15.0%
!! AAAAATTAAAGAGAAATACT+AGG + chr2_3:20543433-20543452 None:intergenic 15.0%
!!! TAGTATTTCTCTTTAATTTT+TGG - chr2_3:20543432-20543451 Msa0283390:intron 15.0%
!!! ACAATATCTTGATTTTTTAA+AGG - chr2_3:20544796-20544815 Msa0283390:five_prime_UTR 15.0%
!! AAAATGATAGAGGAAAAAAA+TGG - chr2_3:20542468-20542487 Msa0283390:CDS 20.0%
!! TACACAACACAATTATAATA+GGG + chr2_3:20542549-20542568 None:intergenic 20.0%
!! ATACACAACACAATTATAAT+AGG + chr2_3:20542550-20542569 None:intergenic 20.0%
!! TGTATTAAACATTTAGATAG+AGG + chr2_3:20543042-20543061 None:intergenic 20.0%
!!! GCTTATTCTTTTATTTGTTT+TGG - chr2_3:20543524-20543543 Msa0283390:intron 20.0%
!!! GATTCTAACTTTGAAAATTT+GGG + chr2_3:20543937-20543956 None:intergenic 20.0%
!!! AGATTCTAACTTTGAAAATT+TGG + chr2_3:20543938-20543957 None:intergenic 20.0%
!! GCAAAATAGAAAAAAATAAC+TGG + chr2_3:20544094-20544113 None:intergenic 20.0%
!! AAAATCTTCAAAAACAAAAC+TGG + chr2_3:20544417-20544436 None:intergenic 20.0%
!! TACAATTGTTAGAATCTATA+TGG - chr2_3:20544582-20544601 Msa0283390:CDS 20.0%
!! TGGTTTCTTAACTAAAATAA+GGG - chr2_3:20544678-20544697 Msa0283390:CDS 20.0%
!!! AATACAAACATACTACTTTT+AGG + chr2_3:20544769-20544788 None:intergenic 20.0%
!! TAAAAGTAGTATGTTTGTAT+TGG - chr2_3:20544768-20544787 Msa0283390:five_prime_UTR 20.0%
! TAATTTCAGTATCAATGCTA+TGG - chr2_3:20542640-20542659 Msa0283390:intron 25.0%
! CAAGTGAGTATAACTTTAAA+AGG + chr2_3:20542783-20542802 None:intergenic 25.0%
! TTAAAGTTATACTCACTTGT+TGG - chr2_3:20542784-20542803 Msa0283390:intron 25.0%
! AAAAAATTGCAAAGTGCAAT+CGG + chr2_3:20542869-20542888 None:intergenic 25.0%
! CTTATTATTTGATACTGAGT+AGG - chr2_3:20543246-20543265 Msa0283390:intron 25.0%
! TAAGTAAAAGGTTAACATAC+AGG + chr2_3:20543277-20543296 None:intergenic 25.0%
! AGCTTCAATAGTTAAGTAAA+AGG + chr2_3:20543289-20543308 None:intergenic 25.0%
! TAACTCATTATATCTCTGTA+TGG - chr2_3:20543669-20543688 Msa0283390:intron 25.0%
! AACTCATTATATCTCTGTAT+GGG - chr2_3:20543670-20543689 Msa0283390:intron 25.0%
! TAAAATACAAAGTCAGAATG+AGG + chr2_3:20543914-20543933 None:intergenic 25.0%
! TTCTGTGTTAATATTTGTGA+TGG - chr2_3:20544647-20544666 Msa0283390:CDS 25.0%
! TCTGTGTTAATATTTGTGAT+GGG - chr2_3:20544648-20544667 Msa0283390:CDS 25.0%
! GTGGTTTCTTAACTAAAATA+AGG - chr2_3:20544677-20544696 Msa0283390:CDS 25.0%
AATTTGTGTGTTTCAGCAAA+AGG - chr2_3:20542228-20542247 Msa0283390:three_prime_UTR 30.0%
! CTAAGCTTTTGTTCATTATC+AGG - chr2_3:20542311-20542330 Msa0283390:three_prime_UTR 30.0%
!!! CTGTTTTGGAAAAATGATAG+AGG - chr2_3:20542458-20542477 Msa0283390:CDS 30.0%
! GCAAAATCTTCCTTGATTTT+GGG + chr2_3:20542667-20542686 None:intergenic 30.0%
! TGCAAAATCTTCCTTGATTT+TGG + chr2_3:20542668-20542687 None:intergenic 30.0%
! TTAGTTGATGTATTTGTCGA+TGG - chr2_3:20542746-20542765 Msa0283390:intron 30.0%
! TAATTTTGCAGTGCTTATGT+TGG + chr2_3:20543017-20543036 None:intergenic 30.0%
CTAAACCAGTTGGTTATAAT+GGG + chr2_3:20543326-20543345 None:intergenic 30.0%
ACTAAACCAGTTGGTTATAA+TGG + chr2_3:20543327-20543346 None:intergenic 30.0%
! TTTAGCTTAGATATAAGGTG+TGG - chr2_3:20543391-20543410 Msa0283390:intron 30.0%
!!! TCTCTTTAATTTTTGGCTTC+TGG - chr2_3:20543439-20543458 Msa0283390:intron 30.0%
ATGTGTGAAGTGAAGTAAAA+AGG + chr2_3:20543465-20543484 None:intergenic 30.0%
ATACTCTTATCTTCTAGACT+CGG - chr2_3:20543483-20543502 Msa0283390:intron 30.0%
!! GGTGCTATTATTATTGTTGA+AGG - chr2_3:20544060-20544079 Msa0283390:intron 30.0%
CTGACATAATATTCAATTGC+AGG - chr2_3:20544116-20544135 Msa0283390:intron 30.0%
AATATTCAATTGCAGGTTCT+TGG - chr2_3:20544123-20544142 Msa0283390:intron 30.0%
ATATTCAATTGCAGGTTCTT+GGG - chr2_3:20544124-20544143 Msa0283390:intron 30.0%
AAGAGAAATCACTATGTTGA+AGG - chr2_3:20544273-20544292 Msa0283390:intron 30.0%
ACATCAATGAACTCATTAGA+AGG - chr2_3:20544322-20544341 Msa0283390:intron 30.0%
ACTCATTAGAAGGATGAATT+AGG - chr2_3:20544332-20544351 Msa0283390:intron 30.0%
! TAGAAGGATGAATTAGGTTT+TGG - chr2_3:20544338-20544357 Msa0283390:intron 30.0%
CTTATTTGTCAATGATGACT+TGG - chr2_3:20544510-20544529 Msa0283390:intron 30.0%
AACACTTGAACTCAACATAA+AGG - chr2_3:20544722-20544741 Msa0283390:CDS 30.0%
ACACTTGAACTCAACATAAA+GGG - chr2_3:20544723-20544742 Msa0283390:CDS 30.0%
AAGGGAGGAGTAATTTATTA+AGG - chr2_3:20544741-20544760 Msa0283390:exon 30.0%
!! ACAACATCGATTCACTGAAA+TGG - chr2_3:20541949-20541968 Msa0283390:three_prime_UTR 35.0%
CAAAACAGATTCAGGAACAA+CGG + chr2_3:20541993-20542012 None:intergenic 35.0%
TGCTTCTTCAAAACAGATTC+AGG + chr2_3:20542001-20542020 None:intergenic 35.0%
!!! CTGTTTTGAAGAAGCAGAAA+AGG - chr2_3:20542005-20542024 Msa0283390:three_prime_UTR 35.0%
! TTCAGCAAAAGGAGTTGATT+AGG - chr2_3:20542239-20542258 Msa0283390:three_prime_UTR 35.0%
ATGAACAAAAGCTTAGCTTC+AGG + chr2_3:20542307-20542326 None:intergenic 35.0%
TTGTTCATTATCAGGATCAG+AGG - chr2_3:20542319-20542338 Msa0283390:three_prime_UTR 35.0%
TGTTCATTATCAGGATCAGA+GGG - chr2_3:20542320-20542339 Msa0283390:three_prime_UTR 35.0%
!! TACAAGCTTGTTCTCTGTTT+TGG - chr2_3:20542444-20542463 Msa0283390:CDS 35.0%
CAATAACAATTAACCCACAC+AGG + chr2_3:20542573-20542592 None:intergenic 35.0%
! CTGTGTGGGTTAATTGTTAT+TGG - chr2_3:20542571-20542590 Msa0283390:intron 35.0%
ATGCAATGCACCTTCAATTA+AGG - chr2_3:20542929-20542948 Msa0283390:CDS 35.0%
TGCAATGCACCTTCAATTAA+GGG - chr2_3:20542930-20542949 Msa0283390:CDS 35.0%
TGAATTGCTCCCTTAATTGA+AGG + chr2_3:20542942-20542961 None:intergenic 35.0%
ATTCACCCATTATAACCAAC+TGG - chr2_3:20543318-20543337 Msa0283390:intron 35.0%
AAAATTGACGATGACAAGGA+TGG + chr2_3:20543353-20543372 None:intergenic 35.0%
TCAGAAAATTGACGATGACA+AGG + chr2_3:20543357-20543376 None:intergenic 35.0%
TTGAGCCATATGTTACCTAT+GGG - chr2_3:20543611-20543630 Msa0283390:intron 35.0%
TCTCTGACACTCTTCAAATT+GGG + chr2_3:20543729-20543748 None:intergenic 35.0%
TTCTCTGACACTCTTCAAAT+TGG + chr2_3:20543730-20543749 None:intergenic 35.0%
AGATAACTCTATCATCGAAC+AGG - chr2_3:20543803-20543822 Msa0283390:intron 35.0%
TGTGTAATCGAATGAAAGCA+GGG + chr2_3:20543852-20543871 None:intergenic 35.0%
AATAATAGCACCACCATACA+CGG + chr2_3:20544052-20544071 None:intergenic 35.0%
ATTCATGAGATCCTTACTGT+TGG - chr2_3:20544177-20544196 Msa0283390:intron 35.0%
AGAAATCACTATGTTGAAGG+TGG - chr2_3:20544276-20544295 Msa0283390:intron 35.0%
!! GTGTCGTTTAAGGTTGATTA+TGG - chr2_3:20544364-20544383 Msa0283390:intron 35.0%
!! TTGTTGTTTGCATTCACGAA+AGG - chr2_3:20544546-20544565 Msa0283390:intron 35.0%
! TATTTGTGATGGGAGAATAG+TGG - chr2_3:20544658-20544677 Msa0283390:CDS 35.0%
TTCTGTTCTGTGAAGGTGAA+CGG + chr2_3:20541921-20541940 None:intergenic 40.0%
! GTTGTAGTTCTGTTCTGTGA+AGG + chr2_3:20541928-20541947 None:intergenic 40.0%
ATTCACTGAAATGGCCAAAG+AGG - chr2_3:20541958-20541977 Msa0283390:three_prime_UTR 40.0%
! AACGGTTTTCACTTCCTCTT+TGG + chr2_3:20541975-20541994 None:intergenic 40.0%
GTATTCCTTAGCGTACTGTT+TGG + chr2_3:20542119-20542138 None:intergenic 40.0%
AAGCGTGAAGCTAAGCTTAA+GGG - chr2_3:20542265-20542284 Msa0283390:three_prime_UTR 40.0%
ATTGTGTTGTGTATCCTGTG+TGG - chr2_3:20542556-20542575 Msa0283390:CDS 40.0%
TTGTGTTGTGTATCCTGTGT+GGG - chr2_3:20542557-20542576 Msa0283390:CDS 40.0%
ATGCTATGGACCCAAAATCA+AGG - chr2_3:20542654-20542673 Msa0283390:intron 40.0%
TAAGGGAGCAATTCATCTAC+TGG - chr2_3:20542947-20542966 Msa0283390:CDS 40.0%
AACCAAGTGTAGAAGACACT+TGG + chr2_3:20542973-20542992 None:intergenic 40.0%
!! CTAGACTCGGTGCAATTTAT+TGG - chr2_3:20543496-20543515 Msa0283390:intron 40.0%
TCGGTGCAATTTATTGGTGT+AGG - chr2_3:20543502-20543521 Msa0283390:intron 40.0%
!! TTTTGGCTGCAGATCTTCAA+TGG - chr2_3:20543541-20543560 Msa0283390:intron 40.0%
AACAGTGAATATGCTCCACA+GGG - chr2_3:20543588-20543607 Msa0283390:intron 40.0%
GTTGAGCCATATGTTACCTA+TGG - chr2_3:20543610-20543629 Msa0283390:intron 40.0%
GCATACCCATAGGTAACATA+TGG + chr2_3:20543619-20543638 None:intergenic 40.0%
TTGAGATTCTGCATACCCAT+AGG + chr2_3:20543629-20543648 None:intergenic 40.0%
AGAGAATTGATCTACAAGCG+TGG - chr2_3:20543744-20543763 Msa0283390:intron 40.0%
TTGATCTACAAGCGTGGTTA+TGG - chr2_3:20543750-20543769 Msa0283390:intron 40.0%
! GTGGTTATGGAAAGGTTGAT+AGG - chr2_3:20543763-20543782 Msa0283390:intron 40.0%
CTGTGTAATCGAATGAAAGC+AGG + chr2_3:20543853-20543872 None:intergenic 40.0%
AATGAGGAGAGATCCTATCT+AGG + chr2_3:20543898-20543917 None:intergenic 40.0%
TTGGAACTGTCTCATCAAGT+TGG + chr2_3:20543969-20543988 None:intergenic 40.0%
TTAGACAAAGTCACGCAATC+TGG + chr2_3:20544006-20544025 None:intergenic 40.0%
ATAATAGCACCACCATACAC+GGG + chr2_3:20544051-20544070 None:intergenic 40.0%
!! AAAGGAGCTTTGAGCTTGAA+AGG + chr2_3:20544237-20544256 None:intergenic 40.0%
TTTCAAGCTCAAAGCTCCTT+TGG - chr2_3:20544236-20544255 Msa0283390:intron 40.0%
TTCAAGCTCAAAGCTCCTTT+GGG - chr2_3:20544237-20544256 Msa0283390:intron 40.0%
!!! GTTTTGGCATGTGTCGTTTA+AGG - chr2_3:20544354-20544373 Msa0283390:intron 40.0%
AAAACTTTCACTGCACTTGC+AGG + chr2_3:20544488-20544507 None:intergenic 40.0%
AAAGTGAAGCAGGGAATGTA+CGG - chr2_3:20544617-20544636 Msa0283390:CDS 40.0%
CTTGAACTCAACATAAAGGG+AGG - chr2_3:20544726-20544745 Msa0283390:CDS 40.0%
GAAGAAGCAGAAAAGGAACG+AGG - chr2_3:20542012-20542031 Msa0283390:three_prime_UTR 45.0%
AGCAGAAAAGGAACGAGGAA+TGG - chr2_3:20542017-20542036 Msa0283390:three_prime_UTR 45.0%
GCAGAAAAGGAACGAGGAAT+GGG - chr2_3:20542018-20542037 Msa0283390:three_prime_UTR 45.0%
!! ATGGGCATTGGTGAAGAAAC+AGG - chr2_3:20542036-20542055 Msa0283390:three_prime_UTR 45.0%
AGAAGAAGAGAGCTGAAACC+AGG - chr2_3:20542074-20542093 Msa0283390:three_prime_UTR 45.0%
CTGAAACCAGGAAGCTTATC+TGG - chr2_3:20542086-20542105 Msa0283390:three_prime_UTR 45.0%
TCTACTCCAGATAAGCTTCC+TGG + chr2_3:20542095-20542114 None:intergenic 45.0%
TAGAGCCAAACAGTACGCTA+AGG - chr2_3:20542111-20542130 Msa0283390:three_prime_UTR 45.0%
CGCTAAGGAATACGATGACT+CGG - chr2_3:20542126-20542145 Msa0283390:three_prime_UTR 45.0%
GAAGCGTGAAGCTAAGCTTA+AGG - chr2_3:20542264-20542283 Msa0283390:three_prime_UTR 45.0%
TTTGCAGTGCTTATGTTGGC+AGG + chr2_3:20543013-20543032 None:intergenic 45.0%
GGATGGAGAACTAAACCAGT+TGG + chr2_3:20543336-20543355 None:intergenic 45.0%
CAACAGTGAATATGCTCCAC+AGG - chr2_3:20543587-20543606 Msa0283390:intron 45.0%
TAACATATGGCTCAACCCTG+TGG + chr2_3:20543606-20543625 None:intergenic 45.0%
CTACAAGCGTGGTTATGGAA+AGG - chr2_3:20543755-20543774 Msa0283390:intron 45.0%
TGAACTGTCCCAACCTAGAT+AGG - chr2_3:20543882-20543901 Msa0283390:intron 45.0%
GGAGAGATCCTATCTAGGTT+GGG + chr2_3:20543893-20543912 None:intergenic 45.0%
AGGAGAGATCCTATCTAGGT+TGG + chr2_3:20543894-20543913 None:intergenic 45.0%
TCTGGACTGTCTCATCAAGT+TGG + chr2_3:20543988-20544007 None:intergenic 45.0%
TTGCAGGTTCTTGGGAAACA+TGG - chr2_3:20544132-20544151 Msa0283390:intron 45.0%
TGAAGTGAGGTCCAACAGTA+AGG + chr2_3:20544191-20544210 None:intergenic 45.0%
!! TTGTTTGCCTCTCTGAAGTG+AGG + chr2_3:20544204-20544223 None:intergenic 45.0%
GAGAGGCAAACAACTTCCTA+TGG - chr2_3:20544211-20544230 Msa0283390:intron 45.0%
CTTTGAGCTTGAAAGGCCAT+AGG + chr2_3:20544230-20544249 None:intergenic 45.0%
TTCTTCTTCATGCCACCCAA+AGG + chr2_3:20544255-20544274 None:intergenic 45.0%
! TATGTTGAAGGTGGTGATGC+TGG - chr2_3:20544285-20544304 Msa0283390:intron 45.0%
TCTCTGAGTGAAAGTGAAGC+AGG - chr2_3:20544607-20544626 Msa0283390:CDS 45.0%
CTCTGAGTGAAAGTGAAGCA+GGG - chr2_3:20544608-20544627 Msa0283390:CDS 45.0%
!!! TATAAAATATTTTAATACAA+TGG - chr2_3:20543176-20543195 Msa0283390:intron 5.0%
!!! ATAAAATATTTTAATACAAT+GGG - chr2_3:20543177-20543196 Msa0283390:intron 5.0%
AAGGAACGAGGAATGGGCAT+TGG - chr2_3:20542024-20542043 Msa0283390:three_prime_UTR 50.0%
CGTGAAGCTAAGCTTAAGGG+TGG - chr2_3:20542268-20542287 Msa0283390:three_prime_UTR 50.0%
GGCCAAGTGTCTTCTACACT+TGG - chr2_3:20542968-20542987 Msa0283390:CDS 50.0%
CTTGACCTATGTCCCGTGTA+TGG - chr2_3:20544036-20544055 Msa0283390:CDS 50.0%
TGTTGGACCTCACTTCAGAG+AGG - chr2_3:20544194-20544213 Msa0283390:intron 50.0%
AAGCTCAAAGCTCCTTTGGG+TGG - chr2_3:20544240-20544259 Msa0283390:intron 50.0%
! AAGGTGGTGATGCTGGTAAC+AGG - chr2_3:20544292-20544311 Msa0283390:intron 50.0%
! AGGTGGTGATGCTGGTAACA+GGG - chr2_3:20544293-20544312 Msa0283390:intron 50.0%
TGAAGGTGAACGGCGCTTCA+AGG + chr2_3:20541911-20541930 None:intergenic 55.0%
GCAGCTTCTGCGATTGAGAC+AGG - chr2_3:20542685-20542704 Msa0283390:intron 55.0%
GACCTATGTCCCGTGTATGG+TGG - chr2_3:20544039-20544058 Msa0283390:CDS 55.0%
CACCACCATACACGGGACAT+AGG + chr2_3:20544044-20544063 None:intergenic 55.0%
! TGGTGATGCTGGTAACAGGG+AGG - chr2_3:20544296-20544315 Msa0283390:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 20541893 20544824 20541893 ID=Msa0283390;Name=Msa0283390
chr2_3 mRNA 20541893 20544824 20541893 ID=Msa0283390-mRNA-1;Parent=Msa0283390;Name=Msa0283390-mRNA-1;_AED=0.07;_eAED=0.07;_QI=75|1|1|1|1|1|6|471|244
chr2_3 exon 20541893 20542579 20541893 ID=Msa0283390-mRNA-1:exon:8123;Parent=Msa0283390-mRNA-1
chr2_3 exon 20542893 20542995 20542893 ID=Msa0283390-mRNA-1:exon:8122;Parent=Msa0283390-mRNA-1
chr2_3 exon 20543085 20543164 20543085 ID=Msa0283390-mRNA-1:exon:8121;Parent=Msa0283390-mRNA-1
chr2_3 exon 20544011 20544068 20544011 ID=Msa0283390-mRNA-1:exon:8120;Parent=Msa0283390-mRNA-1
chr2_3 exon 20544376 20544473 20544376 ID=Msa0283390-mRNA-1:exon:8119;Parent=Msa0283390-mRNA-1
chr2_3 exon 20544570 20544824 20544570 ID=Msa0283390-mRNA-1:exon:8118;Parent=Msa0283390-mRNA-1
chr2_3 five_prime_UTR 20544750 20544824 20544750 ID=Msa0283390-mRNA-1:five_prime_utr;Parent=Msa0283390-mRNA-1
chr2_3 CDS 20544570 20544749 20544570 ID=Msa0283390-mRNA-1:cds;Parent=Msa0283390-mRNA-1
chr2_3 CDS 20544376 20544473 20544376 ID=Msa0283390-mRNA-1:cds;Parent=Msa0283390-mRNA-1
chr2_3 CDS 20544011 20544068 20544011 ID=Msa0283390-mRNA-1:cds;Parent=Msa0283390-mRNA-1
chr2_3 CDS 20543085 20543164 20543085 ID=Msa0283390-mRNA-1:cds;Parent=Msa0283390-mRNA-1
chr2_3 CDS 20542893 20542995 20542893 ID=Msa0283390-mRNA-1:cds;Parent=Msa0283390-mRNA-1
chr2_3 CDS 20542364 20542579 20542364 ID=Msa0283390-mRNA-1:cds;Parent=Msa0283390-mRNA-1
chr2_3 three_prime_UTR 20541893 20542363 20541893 ID=Msa0283390-mRNA-1:three_prime_utr;Parent=Msa0283390-mRNA-1
Gene Sequence

>Msa0283390

ATGGCCAAAGAGGAAGTGAAAACCGTTGTTCCTGAATCTGTTTTGAAGAAGCAGAAAAGGAACGAGGAATGGGCATTGGTGAAGAAACAGGATCAAGAATCAGCGAAGAAGAAGAGAGCTGAAACCAGGAAGCTTATCTGGAGTAGAGCCAAACAGTACGCTAAGGAATACGATGACTCGCAAAAGGAGTTGATTAGGTTGAAGCGTGAAGCTAAGCTTAAGGGTGGATTTTATGTTGATCCTGAAGCTAAGCTTTTGTTCATTATCAGGATCAGAGGTATCAATGCTATGGACCCAAAATCAAGGAAGATTTTGCAGCTTCTGCGATTGAGACAGATCTTCAATGGTGTCTTTCTCAAAGTGAACAAAGCAACAGTGAATATGCTCCACAGGGTTGAGCCATATGTTACCTATGGTTATCCCAATTTGAAGAGTGTCAGAGAATTGATCTACAAGCGTGGTTATGGAAAGGTTGATAGGCAGAGAATTGCTTTGACAGATAACTCTATCATCGAACAGGTTCTTGGGAAACATGGTATCATTTGCATTGAAGATCTCATTCATGAGATCCTTACTGTTGGACCTCACTTCAGAGAGGCAAACAACTTCCTATGGCCTTTCAAGCTCAAAGCTCCTTTGGGTGGCATGAAGAAGAAGAGAAATCACTATGTTGAAGGTGGTGATGCTGGTAACAGGGAGGACTACATCAATGAACTCATTAGAAGGATGAATTAG
Protein sequence

>Msa0283390

MAKEEVKTVVPESVLKKQKRNEEWALVKKQDQESAKKKRAETRKLIWSRAKQYAKEYDDSQKELIRLKREAKLKGGFYVDPEAKLLFIIRIRGINAMDPKSRKILQLLRLRQIFNGVFLKVNKATVNMLHRVEPYVTYGYPNLKSVRELIYKRGYGKVDRQRIALTDNSIIEQVLGKHGIICIEDLIHEILTVGPHFREANNFLWPFKLKAPLGGMKKKRNHYVEGGDAGNREDYINELIRRMN*