AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0298200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0298200 MtrunA17_Chr2g0321571 100.000 107 0 0 1 107 1 107 6.46e-75 216
Msa0298200 MtrunA17_Chr4g0035271 99.065 107 1 0 1 107 1 107 2.02e-74 215
Msa0298200 MtrunA17_Chr6g0455051 75.556 45 2 1 26 70 6 41 1.05e-16 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0298200 AT2G21580.1 91.667 108 8 1 1 107 1 108 2.42e-53 162
Msa0298200 AT4G39200.1 88.889 108 11 1 1 107 1 108 9.22e-52 158
Msa0298200 AT2G21580.2 87.963 108 11 2 1 107 1 107 1.09e-51 158
Msa0298200 AT4G39200.2 87.037 108 12 2 1 107 1 107 4.24e-50 154
Msa0298200 AT4G34555.1 88.785 107 11 1 1 106 1 107 9.74e-50 153
Msa0298200 AT2G16360.1 85.714 105 14 1 2 105 2 106 3.04e-46 144

Find 38 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAGTGATGAGTTTGTATT+TGG 0.207961 2_3:-52124956 None:intergenic
TGCTTGCCGCAGAGAAGATT+TGG 0.313854 2_3:-52124652 None:intergenic
GAAAGCAAAAGAAGAAGAAA+TGG 0.344636 2_3:+52124851 Msa0298200:CDS
GGGGTTGGTGGTTACCATTA+GGG 0.358777 2_3:-52124691 None:intergenic
CAGTCCTCTCTGATCGTCTC+AGG 0.361688 2_3:+52124980 Msa0298200:CDS
CGCAGAGAAGATTTGGAAGA+AGG 0.369919 2_3:-52124645 None:intergenic
TGATGGCAAGAGGATTGATT+AGG 0.387796 2_3:+52125124 Msa0298200:CDS
TCATCAAAGCCGGCGAAATC+AGG 0.410202 2_3:+52124823 Msa0298200:CDS
GGTGGTGGTGCTGCCTTCTT+CGG 0.414824 2_3:-52124799 None:intergenic
TCGTGCAATCAGAGATTTGA+TGG 0.416147 2_3:+52125107 Msa0298200:CDS
TCATCAACTCCAAACCCTAA+TGG 0.421313 2_3:+52124677 Msa0298200:exon
TGGTGGTTACCATTAGGGTT+TGG 0.424277 2_3:-52124686 None:intergenic
TGAAACCGCCTATGTATTAG+TGG 0.440250 2_3:-52125187 None:intergenic
CTAATACATAGGCGGTTTCA+AGG 0.473470 2_3:+52125190 Msa0298200:exon
AACATGGTCTTGTTTGATCA+AGG 0.477202 2_3:+52124904 Msa0298200:CDS
CAAAGGTAAACAAAAGGAAA+AGG 0.503611 2_3:+52124876 Msa0298200:CDS
TTCAGTGAGAAGCTTATCAT+AGG 0.513766 2_3:-52124929 None:intergenic
GATTTCGCCGGCTTTGATGA+CGG 0.516878 2_3:-52124820 None:intergenic
ATGGAGCAAAGGTAAACAAA+AGG 0.525529 2_3:+52124870 Msa0298200:CDS
ACCACCACCGTCATCAAAGC+CGG 0.527384 2_3:+52124813 Msa0298200:CDS
CACTAGAGCCACTAATACAT+AGG 0.531061 2_3:+52125179 Msa0298200:CDS
TTTGTTGTAGGCACCGAAGA+AGG 0.534954 2_3:+52124786 Msa0298200:intron
GGAGTGATGAGTTTGTATTT+GGG 0.540872 2_3:-52124955 None:intergenic
GCAGAGAAGATTTGGAAGAA+GGG 0.551616 2_3:-52124644 None:intergenic
ATCAGAGATTTGATGGCAAG+AGG 0.560553 2_3:+52125114 Msa0298200:CDS
TTTCCTCCTCCTGATTTCGC+CGG 0.569055 2_3:-52124832 None:intergenic
GCCGGCTTTGATGACGGTGG+TGG 0.569126 2_3:-52124814 None:intergenic
AGACGATCAGAGAGGACTGA+AGG 0.570313 2_3:-52124976 None:intergenic
GGCAAGAGGATTGATTAGGT+TGG 0.587429 2_3:+52125128 Msa0298200:CDS
AGTCCTCTCTGATCGTCTCA+GGG 0.592148 2_3:+52124981 Msa0298200:CDS
AAGAAGAAGAAATGGAGCAA+AGG 0.611863 2_3:+52124859 Msa0298200:CDS
AAGCCGGCGAAATCAGGAGG+AGG 0.614520 2_3:+52124829 Msa0298200:CDS
TCAAAGCCGGCGAAATCAGG+AGG 0.640955 2_3:+52124826 Msa0298200:CDS
AAAGGAAAAGGTTAACAACA+TGG 0.683005 2_3:+52124888 Msa0298200:CDS
CTTCTTCCAAATCTTCTCTG+CGG 0.690277 2_3:+52124646 Msa0298200:five_prime_UTR
TTCGCCGGCTTTGATGACGG+TGG 0.725481 2_3:-52124817 None:intergenic
TAGAGCCACTAATACATAGG+CGG 0.728751 2_3:+52125182 Msa0298200:exon
ATACCCTGAGACGATCAGAG+AGG 0.757534 2_3:-52124984 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTGTTTTTAATTTTATTATG+AGG + chr2_3:52125212-52125231 Msa0298200:three_prime_UTR 15.0%
!!! TATGTTTACATTATTGTTTT+TGG + chr2_3:52125339-52125358 Msa0298200:three_prime_UTR 15.0%
!!! ATGTTTACATTATTGTTTTT+GGG + chr2_3:52125340-52125359 Msa0298200:three_prime_UTR 15.0%
!!! TTGTATAATTGATTTGTTTT+AGG + chr2_3:52125367-52125386 Msa0298200:three_prime_UTR 15.0%
!!! GTGTTTTTAATTTTATTATG+AGG + chr2_3:52125212-52125231 Msa0298200:three_prime_UTR 15.0%
!!! TATGTTTACATTATTGTTTT+TGG + chr2_3:52125339-52125358 Msa0298200:three_prime_UTR 15.0%
!!! ATGTTTACATTATTGTTTTT+GGG + chr2_3:52125340-52125359 Msa0298200:three_prime_UTR 15.0%
!!! TTGTATAATTGATTTGTTTT+AGG + chr2_3:52125367-52125386 Msa0298200:three_prime_UTR 15.0%
!!! TTGTAATGTTTTCAGATTAA+TGG + chr2_3:52125072-52125091 Msa0298200:intron 20.0%
!!! TTGATTTTACTCTAAAGTTT+AGG + chr2_3:52125402-52125421 Msa0298200:three_prime_UTR 20.0%
!!! TTGTAATGTTTTCAGATTAA+TGG + chr2_3:52125072-52125091 Msa0298200:intron 20.0%
!!! TTGATTTTACTCTAAAGTTT+AGG + chr2_3:52125402-52125421 Msa0298200:three_prime_UTR 20.0%
!!! TTTTCAGATTAATGGATCTT+TGG + chr2_3:52125080-52125099 Msa0298200:intron 25.0%
!! TTTTATTATGAGGATGTGTT+TGG + chr2_3:52125222-52125241 Msa0298200:three_prime_UTR 25.0%
!!! TTTTCAGATTAATGGATCTT+TGG + chr2_3:52125080-52125099 Msa0298200:intron 25.0%
!! TTTTATTATGAGGATGTGTT+TGG + chr2_3:52125222-52125241 Msa0298200:three_prime_UTR 25.0%
!! GAATTTGATGATGGAAATTG+GGG - chr2_3:52124713-52124732 None:intergenic 30.0%
!! GGAATTTGATGATGGAAATT+GGG - chr2_3:52124714-52124733 None:intergenic 30.0%
!! AGGAATTTGATGATGGAAAT+TGG - chr2_3:52124715-52124734 None:intergenic 30.0%
TAAGGAAAGGAATTTGATGA+TGG - chr2_3:52124722-52124741 None:intergenic 30.0%
!! ACTTCCATTTTCTTTGTTGT+AGG + chr2_3:52124774-52124793 Msa0298200:intron 30.0%
GAAAGCAAAAGAAGAAGAAA+TGG + chr2_3:52124851-52124870 Msa0298200:CDS 30.0%
CAAAGGTAAACAAAAGGAAA+AGG + chr2_3:52124876-52124895 Msa0298200:CDS 30.0%
AAAGGAAAAGGTTAACAACA+TGG + chr2_3:52124888-52124907 Msa0298200:CDS 30.0%
!!! TTTTACTCTAAAGTTTAGGC+AGG + chr2_3:52125406-52125425 Msa0298200:three_prime_UTR 30.0%
!! GAATTTGATGATGGAAATTG+GGG - chr2_3:52124713-52124732 None:intergenic 30.0%
!! GGAATTTGATGATGGAAATT+GGG - chr2_3:52124714-52124733 None:intergenic 30.0%
!! AGGAATTTGATGATGGAAAT+TGG - chr2_3:52124715-52124734 None:intergenic 30.0%
TAAGGAAAGGAATTTGATGA+TGG - chr2_3:52124722-52124741 None:intergenic 30.0%
!! ACTTCCATTTTCTTTGTTGT+AGG + chr2_3:52124774-52124793 Msa0298200:intron 30.0%
GAAAGCAAAAGAAGAAGAAA+TGG + chr2_3:52124851-52124870 Msa0298200:CDS 30.0%
CAAAGGTAAACAAAAGGAAA+AGG + chr2_3:52124876-52124895 Msa0298200:CDS 30.0%
AAAGGAAAAGGTTAACAACA+TGG + chr2_3:52124888-52124907 Msa0298200:CDS 30.0%
CGAGATTTAGATCTAAGGAA+AGG - chr2_3:52124735-52124754 None:intergenic 35.0%
TGCAACGAGATTTAGATCTA+AGG - chr2_3:52124740-52124759 None:intergenic 35.0%
AAGAAGAAGAAATGGAGCAA+AGG + chr2_3:52124859-52124878 Msa0298200:CDS 35.0%
ATGGAGCAAAGGTAAACAAA+AGG + chr2_3:52124870-52124889 Msa0298200:CDS 35.0%
!! AACATGGTCTTGTTTGATCA+AGG + chr2_3:52124904-52124923 Msa0298200:CDS 35.0%
TTCAGTGAGAAGCTTATCAT+AGG - chr2_3:52124932-52124951 None:intergenic 35.0%
GGAGTGATGAGTTTGTATTT+GGG - chr2_3:52124958-52124977 None:intergenic 35.0%
AGGAGTGATGAGTTTGTATT+TGG - chr2_3:52124959-52124978 None:intergenic 35.0%
CGAGATTTAGATCTAAGGAA+AGG - chr2_3:52124735-52124754 None:intergenic 35.0%
TGCAACGAGATTTAGATCTA+AGG - chr2_3:52124740-52124759 None:intergenic 35.0%
AAGAAGAAGAAATGGAGCAA+AGG + chr2_3:52124859-52124878 Msa0298200:CDS 35.0%
ATGGAGCAAAGGTAAACAAA+AGG + chr2_3:52124870-52124889 Msa0298200:CDS 35.0%
!! AACATGGTCTTGTTTGATCA+AGG + chr2_3:52124904-52124923 Msa0298200:CDS 35.0%
TTCAGTGAGAAGCTTATCAT+AGG - chr2_3:52124932-52124951 None:intergenic 35.0%
GGAGTGATGAGTTTGTATTT+GGG - chr2_3:52124958-52124977 None:intergenic 35.0%
AGGAGTGATGAGTTTGTATT+TGG - chr2_3:52124959-52124978 None:intergenic 35.0%
GCAGAGAAGATTTGGAAGAA+GGG - chr2_3:52124647-52124666 None:intergenic 40.0%
CTTCTTCCAAATCTTCTCTG+CGG + chr2_3:52124646-52124665 Msa0298200:five_prime_UTR 40.0%
TCATCAACTCCAAACCCTAA+TGG + chr2_3:52124677-52124696 Msa0298200:exon 40.0%
!! TTGATGATGGAAATTGGGGT+TGG - chr2_3:52124709-52124728 None:intergenic 40.0%
GGTGCCTACAACAAAGAAAA+TGG - chr2_3:52124781-52124800 None:intergenic 40.0%
GAATTGAATCGAAGCGACAA+AGG - chr2_3:52125035-52125054 None:intergenic 40.0%
TCGTGCAATCAGAGATTTGA+TGG + chr2_3:52125107-52125126 Msa0298200:CDS 40.0%
! ATCAGAGATTTGATGGCAAG+AGG + chr2_3:52125114-52125133 Msa0298200:CDS 40.0%
TGATGGCAAGAGGATTGATT+AGG + chr2_3:52125124-52125143 Msa0298200:CDS 40.0%
CACTAGAGCCACTAATACAT+AGG + chr2_3:52125179-52125198 Msa0298200:CDS 40.0%
TAGAGCCACTAATACATAGG+CGG + chr2_3:52125182-52125201 Msa0298200:exon 40.0%
TGAAACCGCCTATGTATTAG+TGG - chr2_3:52125190-52125209 None:intergenic 40.0%
CTAATACATAGGCGGTTTCA+AGG + chr2_3:52125190-52125209 Msa0298200:exon 40.0%
GCAGAGAAGATTTGGAAGAA+GGG - chr2_3:52124647-52124666 None:intergenic 40.0%
CTTCTTCCAAATCTTCTCTG+CGG + chr2_3:52124646-52124665 Msa0298200:five_prime_UTR 40.0%
TCATCAACTCCAAACCCTAA+TGG + chr2_3:52124677-52124696 Msa0298200:exon 40.0%
!! TTGATGATGGAAATTGGGGT+TGG - chr2_3:52124709-52124728 None:intergenic 40.0%
GGTGCCTACAACAAAGAAAA+TGG - chr2_3:52124781-52124800 None:intergenic 40.0%
GAATTGAATCGAAGCGACAA+AGG - chr2_3:52125035-52125054 None:intergenic 40.0%
TCGTGCAATCAGAGATTTGA+TGG + chr2_3:52125107-52125126 Msa0298200:CDS 40.0%
! ATCAGAGATTTGATGGCAAG+AGG + chr2_3:52125114-52125133 Msa0298200:CDS 40.0%
TGATGGCAAGAGGATTGATT+AGG + chr2_3:52125124-52125143 Msa0298200:CDS 40.0%
CACTAGAGCCACTAATACAT+AGG + chr2_3:52125179-52125198 Msa0298200:CDS 40.0%
TAGAGCCACTAATACATAGG+CGG + chr2_3:52125182-52125201 Msa0298200:exon 40.0%
TGAAACCGCCTATGTATTAG+TGG - chr2_3:52125190-52125209 None:intergenic 40.0%
CTAATACATAGGCGGTTTCA+AGG + chr2_3:52125190-52125209 Msa0298200:exon 40.0%
CGCAGAGAAGATTTGGAAGA+AGG - chr2_3:52124648-52124667 None:intergenic 45.0%
TGGTGGTTACCATTAGGGTT+TGG - chr2_3:52124689-52124708 None:intergenic 45.0%
! ATGATGGAAATTGGGGTTGG+TGG - chr2_3:52124706-52124725 None:intergenic 45.0%
!! TTTGTTGTAGGCACCGAAGA+AGG + chr2_3:52124786-52124805 Msa0298200:intron 45.0%
GGCAAGAGGATTGATTAGGT+TGG + chr2_3:52125128-52125147 Msa0298200:CDS 45.0%
CGCAGAGAAGATTTGGAAGA+AGG - chr2_3:52124648-52124667 None:intergenic 45.0%
TGGTGGTTACCATTAGGGTT+TGG - chr2_3:52124689-52124708 None:intergenic 45.0%
! ATGATGGAAATTGGGGTTGG+TGG - chr2_3:52124706-52124725 None:intergenic 45.0%
!! TTTGTTGTAGGCACCGAAGA+AGG + chr2_3:52124786-52124805 Msa0298200:intron 45.0%
GGCAAGAGGATTGATTAGGT+TGG + chr2_3:52125128-52125147 Msa0298200:CDS 45.0%
TGCTTGCCGCAGAGAAGATT+TGG - chr2_3:52124655-52124674 None:intergenic 50.0%
!! GGGGTTGGTGGTTACCATTA+GGG - chr2_3:52124694-52124713 None:intergenic 50.0%
!! TGGGGTTGGTGGTTACCATT+AGG - chr2_3:52124695-52124714 None:intergenic 50.0%
!! GATTTCGCCGGCTTTGATGA+CGG - chr2_3:52124823-52124842 None:intergenic 50.0%
TCATCAAAGCCGGCGAAATC+AGG + chr2_3:52124823-52124842 Msa0298200:CDS 50.0%
TTTCCTCCTCCTGATTTCGC+CGG - chr2_3:52124835-52124854 None:intergenic 50.0%
AGACGATCAGAGAGGACTGA+AGG - chr2_3:52124979-52124998 None:intergenic 50.0%
AGTCCTCTCTGATCGTCTCA+GGG + chr2_3:52124981-52125000 Msa0298200:CDS 50.0%
ATACCCTGAGACGATCAGAG+AGG - chr2_3:52124987-52125006 None:intergenic 50.0%
TGCTTGCCGCAGAGAAGATT+TGG - chr2_3:52124655-52124674 None:intergenic 50.0%
!! GGGGTTGGTGGTTACCATTA+GGG - chr2_3:52124694-52124713 None:intergenic 50.0%
!! TGGGGTTGGTGGTTACCATT+AGG - chr2_3:52124695-52124714 None:intergenic 50.0%
!! GATTTCGCCGGCTTTGATGA+CGG - chr2_3:52124823-52124842 None:intergenic 50.0%
TCATCAAAGCCGGCGAAATC+AGG + chr2_3:52124823-52124842 Msa0298200:CDS 50.0%
TTTCCTCCTCCTGATTTCGC+CGG - chr2_3:52124835-52124854 None:intergenic 50.0%
AGACGATCAGAGAGGACTGA+AGG - chr2_3:52124979-52124998 None:intergenic 50.0%
AGTCCTCTCTGATCGTCTCA+GGG + chr2_3:52124981-52125000 Msa0298200:CDS 50.0%
ATACCCTGAGACGATCAGAG+AGG - chr2_3:52124987-52125006 None:intergenic 50.0%
ACCACCACCGTCATCAAAGC+CGG + chr2_3:52124813-52124832 Msa0298200:CDS 55.0%
TCAAAGCCGGCGAAATCAGG+AGG + chr2_3:52124826-52124845 Msa0298200:CDS 55.0%
CAGTCCTCTCTGATCGTCTC+AGG + chr2_3:52124980-52124999 Msa0298200:CDS 55.0%
ACCACCACCGTCATCAAAGC+CGG + chr2_3:52124813-52124832 Msa0298200:CDS 55.0%
TCAAAGCCGGCGAAATCAGG+AGG + chr2_3:52124826-52124845 Msa0298200:CDS 55.0%
CAGTCCTCTCTGATCGTCTC+AGG + chr2_3:52124980-52124999 Msa0298200:CDS 55.0%
!! GGTGGTGGTGCTGCCTTCTT+CGG - chr2_3:52124802-52124821 None:intergenic 60.0%
!! TTCGCCGGCTTTGATGACGG+TGG - chr2_3:52124820-52124839 None:intergenic 60.0%
AAGCCGGCGAAATCAGGAGG+AGG + chr2_3:52124829-52124848 Msa0298200:CDS 60.0%
!! GGTGGTGGTGCTGCCTTCTT+CGG - chr2_3:52124802-52124821 None:intergenic 60.0%
!! TTCGCCGGCTTTGATGACGG+TGG - chr2_3:52124820-52124839 None:intergenic 60.0%
AAGCCGGCGAAATCAGGAGG+AGG + chr2_3:52124829-52124848 Msa0298200:CDS 60.0%
!! GCCGGCTTTGATGACGGTGG+TGG - chr2_3:52124817-52124836 None:intergenic 65.0%
!! GCCGGCTTTGATGACGGTGG+TGG - chr2_3:52124817-52124836 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 52124632 52125428 52124632 ID=Msa0298200;Name=Msa0298200
chr2_3 mRNA 52124632 52125428 52124632 ID=Msa0298200-mRNA-1;Parent=Msa0298200;Name=Msa0298200-mRNA-1;_AED=0.06;_eAED=0.06;_QI=64|1|1|1|1|1|3|228|107
chr2_3 exon 52124632 52124698 52124632 ID=Msa0298200-mRNA-1:exon:16630;Parent=Msa0298200-mRNA-1
chr2_3 exon 52124796 52125002 52124796 ID=Msa0298200-mRNA-1:exon:16631;Parent=Msa0298200-mRNA-1
chr2_3 exon 52125087 52125428 52125087 ID=Msa0298200-mRNA-1:exon:16632;Parent=Msa0298200-mRNA-1
chr2_3 five_prime_UTR 52124632 52124695 52124632 ID=Msa0298200-mRNA-1:five_prime_utr;Parent=Msa0298200-mRNA-1
chr2_3 CDS 52124696 52124698 52124696 ID=Msa0298200-mRNA-1:cds;Parent=Msa0298200-mRNA-1
chr2_3 CDS 52124796 52125002 52124796 ID=Msa0298200-mRNA-1:cds;Parent=Msa0298200-mRNA-1
chr2_3 CDS 52125087 52125200 52125087 ID=Msa0298200-mRNA-1:cds;Parent=Msa0298200-mRNA-1
chr2_3 three_prime_UTR 52125201 52125428 52125201 ID=Msa0298200-mRNA-1:three_prime_utr;Parent=Msa0298200-mRNA-1
Gene Sequence

>Msa0298200

ATGGCACCGAAGAAGGCAGCACCACCACCGTCATCAAAGCCGGCGAAATCAGGAGGAGGAAAGCAAAAGAAGAAGAAATGGAGCAAAGGTAAACAAAAGGAAAAGGTTAACAACATGGTCTTGTTTGATCAAGGTACCTATGATAAGCTTCTCACTGAAGCTCCCAAATACAAACTCATCACTCCTTCAGTCCTCTCTGATCGTCTCAGGATTAATGGATCTTTGGCACGTCGTGCAATCAGAGATTTGATGGCAAGAGGATTGATTAGGTTGGTGTCTGCTCATTCTAGTCAGCAGATTTACACTAGAGCCACTAATACATAG

Protein sequence

>Msa0298200

MAPKKAAPPPSSKPAKSGGGKQKKKKWSKGKQKEKVNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMARGLIRLVSAHSSQQIYTRATNT*