AlfalfaGEDB | Msa1309050

M. sativa cultivar zhongmu-4 / Msa1309050

KEGG: -
GO: GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
PFAM: PF02298.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309050	KEH17865.1	88.718	195	14	1	56	250	1	187	5.74e-106	314

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309050	sp\|Q41001\|BCP_PEA	58.559	111	42	3	66	175	15	122	1.14e-33	123

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309050	A0A072TWJ3	88.718	195	14	1	56	250	1	187	2.74e-106	314

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0029010	Msa1309050	0.821255	4.448821e-53	-8.615850e-47
Msa0035790	Msa1309050	0.809434	1.895827e-50	-8.615850e-47
Msa0046680	Msa1309050	0.833787	4.363499e-56	-8.615850e-47
Msa0076640	Msa1309050	0.846475	2.121980e-59	-8.615850e-47
Msa0744540	Msa1309050	0.801021	1.095671e-48	-8.615850e-47
Msa0744560	Msa1309050	0.804524	2.072996e-49	-8.615850e-47
Msa0826360	Msa1309050	0.808953	2.403453e-50	-8.615850e-47
Msa0826370	Msa1309050	0.817919	2.568623e-52	-8.615850e-47
Msa0826380	Msa1309050	0.810909	9.118461e-51	-8.615850e-47
Msa0826450	Msa1309050	0.815899	7.296883e-52	-8.615850e-47
Msa0224210	Msa1309050	0.809028	2.316688e-50	-8.615850e-47
Msa1228810	Msa1309050	0.876703	1.130868e-68	-8.615850e-47
Msa1256600	Msa1309050	0.801717	7.891251e-49	-8.615850e-47
Msa1290780	Msa1309050	0.806358	8.549519e-50	-8.615850e-47
Msa1309040	Msa1309050	0.838558	2.678459e-57	-8.615850e-47
Msa1309050	Msa1326290	0.810178	1.311284e-50	-8.615850e-47
Msa1309050	Msa1344330	0.840461	8.576664e-58	-8.615850e-47
Msa1309050	Msa1346010	0.809333	1.992584e-50	-8.615850e-47
Msa1309050	Msa1383790	0.813547	2.421564e-51	-8.615850e-47
Msa1309050	Msa1396160	0.812235	4.694792e-51	-8.615850e-47
Msa1309050	Msa1401110	0.804942	1.695511e-49	-8.615850e-47
Msa1309050	Msa1453600	0.806925	6.490593e-50	-8.615850e-47
Msa1309050	Msa1459930	0.806168	9.375545e-50	-8.615850e-47
Msa0385320	Msa1309050	0.834577	2.766276e-56	-8.615850e-47
Msa0387180	Msa1309050	0.812762	3.602045e-51	-8.615850e-47
Msa0390790	Msa1309050	0.809746	1.624571e-50	-8.615850e-47
Msa0466850	Msa1309050	0.833312	5.732593e-56	-8.615850e-47
Msa0480530	Msa1309050	0.801282	9.689694e-49	-8.615850e-47
Msa0480550	Msa1309050	0.806543	7.817815e-50	-8.615850e-47
Msa0481740	Msa1309050	0.821981	3.024067e-53	-8.615850e-47
Msa0481770	Msa1309050	0.818584	1.816258e-52	-8.615850e-47
Msa1080650	Msa1309050	0.827697	1.355441e-54	-8.615850e-47
Msa0262360	Msa1309050	0.812551	4.005118e-51	-8.615850e-47
Msa0300140	Msa1309050	0.803975	2.697528e-49	-8.615850e-47
Msa1126440	Msa1309050	0.824167	9.345627e-54	-8.615850e-47
Msa1183750	Msa1309050	0.980793	4.779132e-151	-8.615850e-47
Msa1194110	Msa1309050	0.804588	2.010342e-49	-8.615850e-47
Msa0489390	Msa1309050	0.808724	2.691069e-50	-8.615850e-47
Msa0513670	Msa1309050	0.840196	1.005612e-57	-8.615850e-47
Msa0525940	Msa1309050	-0.825681	4.105259e-54	-8.615850e-47
Msa0570460	Msa1309050	0.815665	8.228097e-52	-8.615850e-47
Msa0865810	Msa1309050	0.825923	3.596944e-54	-8.615850e-47
Msa0865830	Msa1309050	0.811211	7.840950e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309050	MtrunA17_Chr8g0335531	88.718	195	14	1	56	250	1	187	5.28e-110	314
Msa1309050	MtrunA17_Chr8g0335571	87.940	199	20	1	56	250	1	199	5.45e-103	297
Msa1309050	MtrunA17_Chr3g0132441	56.303	119	49	2	57	175	5	120	1.79e-36	127
Msa1309050	MtrunA17_Chr3g0132431	47.857	140	66	3	57	192	5	141	6.39e-34	120
Msa1309050	MtrunA17_Chr3g0132411	49.231	130	62	3	57	185	43	169	3.39e-32	115
Msa1309050	MtrunA17_Chr3g0132511	53.097	113	50	3	65	175	11	122	4.13e-30	112
Msa1309050	MtrunA17_Chr2g0323021	39.726	146	83	3	63	207	11	152	3.26e-29	110
Msa1309050	MtrunA17_Chr5g0394321	36.774	155	87	4	64	215	11	157	3.70e-29	108
Msa1309050	MtrunA17_Chr1g0205701	34.211	152	95	3	66	214	12	161	1.93e-27	103
Msa1309050	MtrunA17_Chr3g0132701	47.727	132	54	4	65	182	11	141	5.60e-26	101
Msa1309050	MtrunA17_Chr1g0196801	37.037	135	82	2	76	210	20	151	1.89e-25	98.6
Msa1309050	MtrunA17_Chr6g0481491	40.164	122	68	2	61	179	7	126	4.87e-23	93.2
Msa1309050	MtrunA17_Chr6g0459441	47.059	102	51	2	75	175	22	121	9.93e-23	91.7
Msa1309050	MtrunA17_Chr4g0064041	43.284	134	64	5	65	188	10	141	6.59e-22	93.2
Msa1309050	MtrunA17_Chr4g0064041	46.078	102	48	3	79	175	191	290	3.77e-21	90.9
Msa1309050	MtrunA17_Chr1g0211101	38.255	149	82	6	57	199	9	153	9.45e-22	89.0
Msa1309050	MtrunA17_Chr7g0253641	40.336	119	63	3	59	176	11	122	5.75e-21	88.2
Msa1309050	MtrunA17_Chr7g0253681	38.655	119	71	2	60	177	8	125	4.54e-20	84.0
Msa1309050	MtrunA17_Chr2g0301431	41.667	120	62	5	58	174	9	123	7.24e-20	82.4
Msa1309050	MtrunA17_Chr7g0253701	38.655	119	70	3	60	177	8	124	2.31e-19	82.0
Msa1309050	MtrunA17_Chr7g0253671	38.655	119	70	3	60	177	8	124	2.31e-19	82.0
Msa1309050	MtrunA17_Chr1g0177341	36.937	111	68	2	67	176	13	122	9.28e-19	80.5
Msa1309050	MtrunA17_Chr1g0177331	36.937	111	68	2	67	176	13	122	9.28e-19	80.5
Msa1309050	MtrunA17_Chr2g0331181	36.029	136	75	4	76	202	24	156	2.07e-18	80.5
Msa1309050	MtrunA17_Chr4g0040291	32.934	167	103	3	76	239	22	182	4.94e-18	79.3
Msa1309050	MtrunA17_Chr3g0125761	37.500	120	69	4	56	174	10	124	1.52e-17	76.3
Msa1309050	MtrunA17_Chr6g0483981	35.583	163	95	5	74	229	22	181	1.61e-17	81.6
Msa1309050	MtrunA17_Chr6g0453921	37.410	139	73	6	65	200	24	151	2.39e-17	77.4
Msa1309050	MtrunA17_Chr7g0253631	38.136	118	65	3	59	175	11	121	2.50e-17	78.6
Msa1309050	MtrunA17_Chr6g0453801	30.723	166	106	5	50	208	4	167	3.67e-17	76.6
Msa1309050	MtrunA17_Chr3g0136871	34.483	145	80	4	94	237	47	177	8.58e-17	75.9
Msa1309050	MtrunA17_Chr8g0380901	38.793	116	63	5	60	174	11	119	8.90e-17	74.3
Msa1309050	MtrunA17_Chr2g0306461	40.000	105	58	3	76	177	25	127	1.11e-16	75.9
Msa1309050	MtrunA17_Chr3g0125791	41.346	104	55	4	74	176	28	126	1.22e-16	73.9
Msa1309050	MtrunA17_Chr1g0150131	34.899	149	88	4	56	197	1	147	1.36e-16	75.5
Msa1309050	MtrunA17_Chr8g0360371	37.594	133	72	6	61	185	5	134	2.08e-16	77.0
Msa1309050	MtrunA17_Chr7g0253731	33.065	124	80	3	55	177	2	123	7.73e-16	72.8
Msa1309050	MtrunA17_Chr4g0001571	38.384	99	57	3	76	173	26	121	1.13e-15	72.4
Msa1309050	MtrunA17_Chr7g0253761	35.593	118	68	3	59	175	11	121	1.36e-15	72.4
Msa1309050	MtrunA17_Chr4g0073901	33.607	122	73	4	60	175	10	129	2.69e-15	72.0
Msa1309050	MtrunA17_Chr2g0324721	34.254	181	96	7	76	239	24	198	5.10e-15	71.6
Msa1309050	MtrunA17_Chr4g0033131	38.393	112	58	5	76	179	25	133	3.61e-14	68.6
Msa1309050	MtrunA17_Chr4g0033531	38.393	112	58	5	76	179	25	133	3.61e-14	68.6
Msa1309050	MtrunA17_Chr4g0062341	30.323	155	94	5	63	206	11	162	1.29e-13	67.4
Msa1309050	MtrunA17_Chr4g0062311	30.323	155	94	5	63	206	11	162	1.29e-13	67.4
Msa1309050	MtrunA17_Chr2g0289741	33.071	127	78	4	79	203	27	148	1.59e-13	66.6
Msa1309050	MtrunA17_Chr4g0073921	33.600	125	75	4	60	178	10	132	1.61e-13	68.6
Msa1309050	MtrunA17_Chr4g0041811	31.553	206	116	6	63	246	16	218	6.64e-13	66.2
Msa1309050	MtrunA17_Chr4g0064031	38.144	97	52	5	76	164	25	121	9.07e-13	64.3
Msa1309050	MtrunA17_Chr8g0387804	39.048	105	56	4	76	175	26	127	9.47e-13	63.5
Msa1309050	MtrunA17_Chr4g0039721	30.935	139	87	3	77	207	30	167	1.02e-12	64.7
Msa1309050	MtrunA17_Chr6g0453791	29.762	168	101	7	41	207	1	152	3.02e-12	63.2
Msa1309050	MtrunA17_Chr2g0324711	32.967	182	94	8	46	219	6	167	2.38e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309050	AT2G44790.1	53.704	108	46	1	72	179	25	128	2.41e-34	122
Msa1309050	AT3G60270.1	52.336	107	49	1	72	178	19	123	1.19e-33	120
Msa1309050	AT3G60280.1	53.271	107	48	1	72	178	17	121	5.26e-33	120
Msa1309050	AT2G32300.2	42.169	166	75	5	42	188	5	168	1.60e-32	120
Msa1309050	AT2G32300.1	42.484	153	68	5	41	188	1	138	2.00e-32	119
Msa1309050	AT1G72230.1	47.260	146	71	3	67	208	11	154	5.48e-31	113
Msa1309050	AT3G27200.1	41.803	122	68	2	56	176	3	122	3.53e-27	103
Msa1309050	AT5G07475.1	38.514	148	84	2	58	200	4	149	1.53e-26	102
Msa1309050	AT2G31050.1	42.336	137	70	4	63	192	8	142	1.96e-25	99.8
Msa1309050	AT1G22480.1	47.154	123	63	1	87	209	27	147	1.11e-24	97.1
Msa1309050	AT2G26720.1	39.706	136	73	4	64	192	9	142	1.13e-24	97.8
Msa1309050	AT5G26330.1	36.290	124	74	3	56	176	1	122	5.44e-23	92.8
Msa1309050	AT3G17675.1	39.604	101	58	2	76	175	29	127	1.26e-19	82.4
Msa1309050	AT2G25060.1	38.636	132	70	5	63	185	15	144	1.30e-18	81.3
Msa1309050	AT5G53870.2	35.882	170	91	6	64	221	10	173	6.22e-18	82.4
Msa1309050	AT5G20230.1	38.095	126	68	4	78	195	24	147	6.67e-18	79.7
Msa1309050	AT5G53870.1	35.882	170	91	6	64	221	10	173	1.26e-17	81.6
Msa1309050	AT1G79800.1	36.885	122	69	3	78	194	33	151	8.40e-17	76.6
Msa1309050	AT5G15350.1	34.483	145	72	5	61	193	1	134	1.01e-16	75.9
Msa1309050	AT4G31840.1	29.851	201	101	9	57	248	3	172	1.05e-16	75.9
Msa1309050	AT2G02850.1	39.286	112	62	4	66	176	23	129	1.19e-16	74.3
Msa1309050	AT4G12880.1	36.667	120	70	4	59	175	9	125	5.09e-16	73.2
Msa1309050	AT4G28365.1	32.727	165	98	7	64	219	12	172	1.57e-15	73.2
Msa1309050	AT4G30590.1	44.578	83	43	2	96	177	50	130	2.52e-15	72.4
Msa1309050	AT1G64640.1	31.469	143	77	3	76	214	30	155	2.96e-15	72.4
Msa1309050	AT4G27520.1	35.246	122	70	4	64	178	12	131	1.97e-14	72.4
Msa1309050	AT5G57920.3	33.673	98	62	2	94	190	75	170	2.71e-14	70.1
Msa1309050	AT5G57920.1	33.673	98	62	2	94	190	43	138	3.15e-14	69.3
Msa1309050	AT5G57920.2	33.673	98	62	2	94	190	42	137	4.98e-14	68.6
Msa1309050	AT3G20570.1	28.571	182	106	5	56	215	5	184	1.53e-13	67.8
Msa1309050	AT3G53330.1	34.400	125	76	4	75	195	184	306	2.59e-12	65.9
Msa1309050	AT2G27035.1	31.783	129	74	5	82	208	32	148	2.84e-12	63.5
Msa1309050	AT1G48940.1	32.540	126	76	4	55	173	2	125	1.05e-11	62.4
Msa1309050	AT3G18590.1	33.333	123	73	3	60	176	8	127	2.62e-11	61.2
Msa1309050	AT4G32490.1	27.485	171	99	5	60	207	12	180	3.79e-11	61.6
Msa1309050	AT5G25090.1	27.907	129	84	4	57	178	3	129	7.28e-11	60.1

Find 109 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTGTTTCAGCAATGATTT+TGG	0.095923	8_4:+5456102	Msa1309050:CDS
TCCATCAGCATAGTTCTTTA+TGG	0.149502	8_4:-5455786	None:intergenic
GTTGTTTCCATTTCAATTAT+TGG	0.194435	8_4:-5456312	None:intergenic
TTCTTTCTTTGTTCTATTGT+TGG	0.249783	8_4:+5455338	Msa1309050:CDS
GGACGGTGGGCTGGTGGCGG+TGG	0.250889	8_4:-5451290	None:intergenic
CACCATAATTTATGTACTAT+TGG	0.264888	8_4:+5456163	Msa1309050:three_prime_UTR
CGGTGGGCTGGTGGCGGTGG+AGG	0.283739	8_4:-5451287	None:intergenic
CACTTGTTTGAAAGTCAAAT+TGG	0.304204	8_4:-5451150	None:intergenic
AACTCTATTTCCCAAATTGA+TGG	0.308395	8_4:-5451700	None:intergenic
AACCAATAGTACATAAATTA+TGG	0.310625	8_4:-5456165	None:intergenic
GTGGAATTGTGATTTGAAAT+AGG	0.319098	8_4:-5451183	None:intergenic
AAAATTTCTACCATCAATTT+GGG	0.325344	8_4:+5451690	Msa1309050:CDS
GATTACACCAAATGGGCTTC+TGG	0.335655	8_4:+5455426	Msa1309050:CDS
TCCTCCATTCCTTCACCATC+TGG	0.336136	8_4:+5451219	Msa1309050:five_prime_UTR
GGCTGGTGGCGGTGGAGGAG+AGG	0.340235	8_4:-5451282	None:intergenic
CCATCACCACCAAAGGATAA+TGG	0.368895	8_4:+5456034	Msa1309050:CDS
AAGGAATGGAGGAGGAGGTT+TGG	0.372036	8_4:-5451209	None:intergenic
GAAGGACGGTGGGCTGGTGG+CGG	0.383239	8_4:-5451293	None:intergenic
CATAGCCAATCAACGGAGAT+TGG	0.383517	8_4:-5451656	None:intergenic
CTATGCTGATGGAAATTCTA+AGG	0.389114	8_4:+5455796	Msa1309050:CDS
GGTGGCGGTGGAGGAGAGGG+TGG	0.392104	8_4:-5451278	None:intergenic
TTGGTTGCCAATAATTGAAA+TGG	0.395366	8_4:+5456305	Msa1309050:three_prime_UTR
ATGGAAGGACGGTGGGCTGG+TGG	0.404518	8_4:-5451296	None:intergenic
GTGATGGAAGGACGGTGGGC+TGG	0.426349	8_4:-5451299	None:intergenic
CCATTATCCTTTGGTGGTGA+TGG	0.426718	8_4:-5456034	None:intergenic
ATTCACTTTCTTCAATAAGT+TGG	0.426986	8_4:+5451112	Msa1309050:five_prime_UTR
AAGGAGTTGATTACACCAAA+TGG	0.428315	8_4:+5455418	Msa1309050:CDS
GGAGCTGATGGTGTGGTTGA+TGG	0.434928	8_4:-5455989	None:intergenic
GGTGCCACTTGGAGTTGGGG+TGG	0.440747	8_4:-5455924	None:intergenic
GCTGGTGGCGGTGGAGGAGA+GGG	0.446578	8_4:-5451281	None:intergenic
CTAACAGCTCCATTATCCTT+TGG	0.447592	8_4:-5456043	None:intergenic
GGAAGAAGCTTTGTTGATGA+AGG	0.448393	8_4:-5451358	None:intergenic
AACAACCAATCTCCGTTGAT+TGG	0.450157	8_4:+5451651	Msa1309050:five_prime_UTR
TTCAAAGTTGGTGATAACTT+AGG	0.466246	8_4:+5455456	Msa1309050:CDS
GTAGTTGATGGTGTGGTTGA+TGG	0.466465	8_4:-5455965	None:intergenic
GGTTTCACTTGGTTGCTGCT+TGG	0.470295	8_4:+5451983	Msa1309050:intron
GTGGTTGATGGAGTAGTTGA+TGG	0.471972	8_4:-5455977	None:intergenic
AAGGTTGCATTGACTAAAGC+AGG	0.472415	8_4:+5455815	Msa1309050:CDS
GCGGTGGAGGAGAGGGTGGA+TGG	0.472693	8_4:-5451274	None:intergenic
AAATCAGTTGCAAATGCATA+AGG	0.473584	8_4:-5455366	None:intergenic
CAATCTCCGTTGATTGGCTA+TGG	0.474204	8_4:+5451657	Msa1309050:five_prime_UTR
GAGAGGGTGGATGGTTTGAG+AGG	0.481058	8_4:-5451265	None:intergenic
ATTGGCAACCAAACAACAAA+AGG	0.482887	8_4:-5456294	None:intergenic
CACTGCTTCGTCTTCTCTCG+TGG	0.490362	8_4:-5451609	None:intergenic
GTCATCGCGTCGGCAATTAC+AGG	0.496409	8_4:+5451432	Msa1309050:five_prime_UTR
CCAGGTCATTGTACTAGTAC+TGG	0.496658	8_4:+5455863	Msa1309050:CDS
TGGTGAAGGAATGGAGGAGG+AGG	0.497445	8_4:-5451214	None:intergenic
CTCCTTCCACCAAGTGGATG+AGG	0.499710	8_4:+5455724	Msa1309050:CDS
GAAGTTGAGTTTGTCTCTGA+AGG	0.503772	8_4:-5456010	None:intergenic
AGGAGTTGATTACACCAAAT+GGG	0.504844	8_4:+5455419	Msa1309050:CDS
GGTGATGGTGATGGTGGTGA+TGG	0.505725	8_4:-5451315	None:intergenic
TACTTCATATGCCCTACTCC+AGG	0.512113	8_4:+5455845	Msa1309050:CDS
AACTCCTTGATTCCATCCAC+TGG	0.514824	8_4:-5455403	None:intergenic
GGTGGTGATGGAAGGACGGT+GGG	0.517102	8_4:-5451303	None:intergenic
TTTGCAACTGATTTCACTGT+TGG	0.522709	8_4:+5455375	Msa1309050:CDS
GACTTTCAAACAAGTGACCT+GGG	0.526029	8_4:+5451157	Msa1309050:five_prime_UTR
ACAGCTCCATTATCCTTTGG+TGG	0.526576	8_4:-5456040	None:intergenic
ATGGTGATGGTGGTGATGGA+AGG	0.527650	8_4:-5451311	None:intergenic
TTTCTTCAATAAGTTGGCAG+AGG	0.532125	8_4:+5451118	Msa1309050:five_prime_UTR
CATCAACCTCATCCACTTGG+TGG	0.532981	8_4:-5455730	None:intergenic
TGACTTTCAAACAAGTGACC+TGG	0.537509	8_4:+5451156	Msa1309050:five_prime_UTR
TGATGGTGGTGATGGAAGGA+CGG	0.539859	8_4:-5451307	None:intergenic
GGAGGAGGAGGTTTGGCATG+TGG	0.540051	8_4:-5451202	None:intergenic
CTAGTACAATGACCTGGAGT+AGG	0.540305	8_4:-5455857	None:intergenic
GATGAAGGGTGGTGAGGAAG+AGG	0.541166	8_4:-5451343	None:intergenic
TTTGTCTCTGAAGGAGCTGA+TGG	0.541698	8_4:-5456001	None:intergenic
AGAACCAGATGGTGAAGGAA+TGG	0.542948	8_4:-5451223	None:intergenic
CACGAGAGAAGACGAAGCAG+TGG	0.543749	8_4:+5451610	Msa1309050:five_prime_UTR
CATCAAAAGACTAACACCAT+TGG	0.545497	8_4:-5456071	None:intergenic
GAGGACGATGGTGATGGTGA+TGG	0.547264	8_4:-5451324	None:intergenic
CTCTGAAGGAGCTGATGGTG+TGG	0.549422	8_4:-5455996	None:intergenic
TTCACTGTTGGTGATGCCAG+TGG	0.555149	8_4:+5455387	Msa1309050:CDS
GCCATAAAGAACTATGCTGA+TGG	0.555563	8_4:+5455785	Msa1309050:CDS
AGATGGTGAAGGAATGGAGG+AGG	0.563375	8_4:-5451217	None:intergenic
AACTTCTCCATCACCACCAA+AGG	0.566009	8_4:+5456027	Msa1309050:CDS
GAAGAAGCTTTGTTGATGAA+GGG	0.569350	8_4:-5451357	None:intergenic
AACCTCATCCACTTGGTGGA+AGG	0.569814	8_4:-5455726	None:intergenic
TTTCATCAACCTCATCCACT+TGG	0.574306	8_4:-5455733	None:intergenic
TGGTGGTGATGGAAGGACGG+TGG	0.574425	8_4:-5451304	None:intergenic
TGATGGAGTAGTTGATGGTG+TGG	0.574696	8_4:-5455972	None:intergenic
GATGCCAGTGGATGGAATCA+AGG	0.575971	8_4:+5455399	Msa1309050:CDS
GACGATGGTGATGGTGATGG+TGG	0.589376	8_4:-5451321	None:intergenic
CTGTTGGTGATGCCAGTGGA+TGG	0.591217	8_4:+5455391	Msa1309050:CDS
GATAAAGAACCAGATGGTGA+AGG	0.602240	8_4:-5451228	None:intergenic
TAGTACAATGACCTGGAGTA+GGG	0.606340	8_4:-5455856	None:intergenic
AAAGAAGATAAAGAACCAGA+TGG	0.608797	8_4:-5451234	None:intergenic
ATCATTCCATAGCCAATCAA+CGG	0.609518	8_4:-5451663	None:intergenic
TGATTTGAAATAGGAGACCC+AGG	0.610870	8_4:-5451174	None:intergenic
ACCAGATGGTGAAGGAATGG+AGG	0.612255	8_4:-5451220	None:intergenic
AGCACCACCCCAACTCCAAG+TGG	0.615986	8_4:+5455920	Msa1309050:CDS
AGTTGCAAATGCATAAGGAA+AGG	0.617334	8_4:-5455361	None:intergenic
GAGGAAGAGGACGATGGTGA+TGG	0.617521	8_4:-5451330	None:intergenic
GGTGGTGAGGAAGAGGACGA+TGG	0.620056	8_4:-5451336	None:intergenic
CCAGTACTAGTACAATGACC+TGG	0.623789	8_4:-5455863	None:intergenic
ATATGGCTCCTTCCACCAAG+TGG	0.625567	8_4:+5455718	Msa1309050:CDS
GCCGACGCGATGACTTGCGA+TGG	0.626178	8_4:-5451423	None:intergenic
TTTGTTGATGAAGGGTGGTG+AGG	0.626351	8_4:-5451349	None:intergenic
AGAGAAGACGAAGCAGTGGG+AGG	0.627686	8_4:+5451614	Msa1309050:five_prime_UTR
ACGAGAGAAGACGAAGCAGT+GGG	0.633833	8_4:+5451611	Msa1309050:five_prime_UTR
CTCTCGTGGATCTTCCACGT+CGG	0.637812	8_4:-5451595	None:intergenic
GAAGCTTTGTTGATGAAGGG+TGG	0.638379	8_4:-5451354	None:intergenic
GCAACCAAACAACAAAAGGA+AGG	0.640439	8_4:-5456290	None:intergenic
GGTCATTGTACTAGTACTGG+TGG	0.645353	8_4:+5455866	Msa1309050:CDS
TCCATCGCAAGTCATCGCGT+CGG	0.647463	8_4:+5451422	Msa1309050:five_prime_UTR
TGATGTTTGAAATTCAGCGA+GGG	0.663711	8_4:-5451379	None:intergenic
GGAGCTGTTAGTGTTTCCAA+TGG	0.664368	8_4:+5456055	Msa1309050:CDS
TTGATGTTTGAAATTCAGCG+AGG	0.702291	8_4:-5451380	None:intergenic
ATTGAAGAAAGTGAATCACA+TGG	0.702331	8_4:-5451105	None:intergenic
TTATGATTGAGAAGCCGACG+TGG	0.739864	8_4:+5451581	Msa1309050:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATAAGATTTTATAGAATAA+TGG	-	chr8_4:5452628-5452647	None:intergenic	10.0%
!!!	ATTATTAAAATGTATTTCTA+CGG	-	chr8_4:5454740-5454759	None:intergenic	10.0%
!!	AATTAAATTTAAATTCAACA+TGG	+	chr8_4:5455118-5455137	Msa1309050:intron	10.0%
!!!	ATATTATGTTTTTTCTTATT+TGG	+	chr8_4:5455546-5455565	Msa1309050:intron	10.0%
!!!	ATGTTTTCTTTTTTAATTAA+AGG	-	chr8_4:5456234-5456253	None:intergenic	10.0%
!!	TTTATGTTATTAACATTGTA+AGG	+	chr8_4:5451931-5451950	Msa1309050:intron	15.0%
!!!	GTTTTTTTGTTTTTGTTTTA+TGG	+	chr8_4:5452470-5452489	Msa1309050:intron	15.0%
!!!	ATAAGATATTTTTGTTTCAT+GGG	-	chr8_4:5452894-5452913	None:intergenic	15.0%
!!!	AATAAGATATTTTTGTTTCA+TGG	-	chr8_4:5452895-5452914	None:intergenic	15.0%
!!!	AATATAGAATATTCTGAAAT+AGG	-	chr8_4:5453017-5453036	None:intergenic	15.0%
!!	AATATTCTATATTACATCTT+TGG	+	chr8_4:5453024-5453043	Msa1309050:intron	15.0%
!!	ATATTCTATATTACATCTTT+GGG	+	chr8_4:5453025-5453044	Msa1309050:intron	15.0%
!!	TAAAACTTATTTATTTAGAC+AGG	+	chr8_4:5453918-5453937	Msa1309050:intron	15.0%
!!!	CAATTTTTGTTTTATGATTT+TGG	+	chr8_4:5454308-5454327	Msa1309050:intron	15.0%
!!	TTAAAAAAGAAAATAGACAT+TGG	-	chr8_4:5454543-5454562	None:intergenic	15.0%
!!!	ATATTTTGTTATTAAATGTC+TGG	+	chr8_4:5454670-5454689	Msa1309050:intron	15.0%
!!!	TTTTGAGAATTAAAGTTTAT+AGG	+	chr8_4:5455294-5455313	Msa1309050:intron	15.0%
!!	AAAAATTTCTACCATCAATT+TGG	+	chr8_4:5451689-5451708	Msa1309050:CDS	20.0%
!!	AAAATTTCTACCATCAATTT+GGG	+	chr8_4:5451690-5451709	Msa1309050:CDS	20.0%
!!!	TTTTTCATGTTTGATGATTT+TGG	+	chr8_4:5451818-5451837	Msa1309050:intron	20.0%
!!	CTATCAATATTAAATACAGT+TGG	+	chr8_4:5452419-5452438	Msa1309050:intron	20.0%
!!	ATATAAACATGTATGTTAGA+AGG	-	chr8_4:5452501-5452520	None:intergenic	20.0%
!!	AGTATAACAGAGTTATAAAA+AGG	-	chr8_4:5452588-5452607	None:intergenic	20.0%
!!!	CAAATGTTTATGATTTTATC+AGG	+	chr8_4:5452711-5452730	Msa1309050:intron	20.0%
!!!	GAAGTTTTTAAAGATGATAA+AGG	-	chr8_4:5452936-5452955	None:intergenic	20.0%
!!	TACAATTTATGCACTAAATA+TGG	+	chr8_4:5453783-5453802	Msa1309050:intron	20.0%
!!!	ATTGAATCATATTTTTGAAC+AGG	-	chr8_4:5453837-5453856	None:intergenic	20.0%
!!	TAAGCAAGTTAAGAAATTAA+AGG	-	chr8_4:5453892-5453911	None:intergenic	20.0%
!!!	TATGCTTATGTTTATGATTT+TGG	+	chr8_4:5453994-5454013	Msa1309050:intron	20.0%
!!!	TAATTTTTTGCATTCATTCA+AGG	+	chr8_4:5454347-5454366	Msa1309050:intron	20.0%
!!	AACATAAACTGTTATGAATT+CGG	-	chr8_4:5454585-5454604	None:intergenic	20.0%
!!!	TAAGCAAAAATTGACTTTTT+AGG	-	chr8_4:5454992-5455011	None:intergenic	20.0%
!!	TATTTCAGTATATAAACGAA+AGG	-	chr8_4:5455523-5455542	None:intergenic	20.0%
!!	AACCAATAGTACATAAATTA+TGG	-	chr8_4:5456168-5456187	None:intergenic	20.0%
!!!	AGAAAACATTTCTTCTTTTT+AGG	+	chr8_4:5456245-5456264	Msa1309050:three_prime_UTR	20.0%
!	ATTCACTTTCTTCAATAAGT+TGG	+	chr8_4:5451112-5451131	Msa1309050:five_prime_UTR	25.0%
!	CATAAAATGGAAAACGAAAA+AGG	-	chr8_4:5451566-5451585	None:intergenic	25.0%
!	GAGAGTAATATATAAAAGGT+TGG	-	chr8_4:5451891-5451910	None:intergenic	25.0%
!	GTTGGAGAGTAATATATAAA+AGG	-	chr8_4:5451895-5451914	None:intergenic	25.0%
!!!	TTTTGTTGTGTTTGTTTTGT+AGG	+	chr8_4:5451962-5451981	Msa1309050:intron	25.0%
!	TTTGCTTCAAAGTAATGATT+TGG	+	chr8_4:5452045-5452064	Msa1309050:intron	25.0%
!!	AATTTTCATGTTCCTTATGT+TGG	+	chr8_4:5452124-5452143	Msa1309050:intron	25.0%
!	AAAGATAATTCACCAACATA+AGG	-	chr8_4:5452139-5452158	None:intergenic	25.0%
!	ATAAATGAAAGTAAGCACAA+AGG	-	chr8_4:5452193-5452212	None:intergenic	25.0%
!!!	GTGTTTTGAATTGTTTGTAT+TGG	+	chr8_4:5452239-5452258	Msa1309050:intron	25.0%
!	ATAGATTAAGAACAGAACAA+TGG	-	chr8_4:5452403-5452422	None:intergenic	25.0%
!	TAACTCTGTTATACTATTTG+TGG	+	chr8_4:5452593-5452612	Msa1309050:intron	25.0%
!	AACTCTGTTATACTATTTGT+GGG	+	chr8_4:5452594-5452613	Msa1309050:intron	25.0%
!	ATCTTATTATGAACTCTTTG+TGG	+	chr8_4:5452640-5452659	Msa1309050:intron	25.0%
!	TCTTATTATGAACTCTTTGT+GGG	+	chr8_4:5452641-5452660	Msa1309050:intron	25.0%
!	TTCTGTACTAAATCATGATT+AGG	+	chr8_4:5452771-5452790	Msa1309050:intron	25.0%
!!!	GTTTTTAAAGATGATAAAGG+TGG	-	chr8_4:5452933-5452952	None:intergenic	25.0%
!!!	ATAGAATATTCTGAAATAGG+CGG	-	chr8_4:5453014-5453033	None:intergenic	25.0%
!!	CAAACTAATTTTGTATGTGT+AGG	+	chr8_4:5453072-5453091	Msa1309050:intron	25.0%
!	AAAATCAGAACAAAACGAAA+AGG	-	chr8_4:5453279-5453298	None:intergenic	25.0%
!!!	AACTGTTGTTTTACTCAAAA+TGG	-	chr8_4:5453650-5453669	None:intergenic	25.0%
!	GTTAGTCCTTGAAAAATTTA+AGG	+	chr8_4:5454391-5454410	Msa1309050:intron	25.0%
!	TATCACTAATATACCTTGAA+TGG	-	chr8_4:5454421-5454440	None:intergenic	25.0%
!!!	ACATTTTTAGGAGTAAAGTT+TGG	+	chr8_4:5454482-5454501	Msa1309050:intron	25.0%
!	TAAATGTCAATGAACCATTT+GGG	-	chr8_4:5454611-5454630	None:intergenic	25.0%
!	TTAAATGTCAATGAACCATT+TGG	-	chr8_4:5454612-5454631	None:intergenic	25.0%
!	TTTAAGTGAAAATTAAGAGC+CGG	+	chr8_4:5454627-5454646	Msa1309050:intron	25.0%
!	AATAGCATGTGATTACTAAT+CGG	-	chr8_4:5454773-5454792	None:intergenic	25.0%
!	TGCTATTGTAGAAATTGAAA+CGG	+	chr8_4:5454786-5454805	Msa1309050:intron	25.0%
!!	TTTTTGCTTATAATAGTGAC+CGG	-	chr8_4:5455002-5455021	None:intergenic	25.0%
!!	TTTTTGCTTATAATAGTGAC+CGG	+	chr8_4:5455002-5455021	Msa1309050:intron	25.0%
!	TTCTTTCTTTGTTCTATTGT+TGG	+	chr8_4:5455338-5455357	Msa1309050:CDS	25.0%
!!!	GTTTGATAATGCTAAATTTG+AGG	+	chr8_4:5455582-5455601	Msa1309050:intron	25.0%
!	GAAAAAACAGTGTTCAAATA+TGG	+	chr8_4:5455701-5455720	Msa1309050:intron	25.0%
!!	TTTGTTTCAGCAATGATTTT+GGG	+	chr8_4:5456103-5456122	Msa1309050:CDS	25.0%
!	CACCATAATTTATGTACTAT+TGG	+	chr8_4:5456163-5456182	Msa1309050:three_prime_UTR	25.0%
!!	GTTGTTTCCATTTCAATTAT+TGG	-	chr8_4:5456315-5456334	None:intergenic	25.0%
	CACTTGTTTGAAAGTCAAAT+TGG	-	chr8_4:5451153-5451172	None:intergenic	30.0%
!	GTGGAATTGTGATTTGAAAT+AGG	-	chr8_4:5451186-5451205	None:intergenic	30.0%
	AAAGAAGATAAAGAACCAGA+TGG	-	chr8_4:5451237-5451256	None:intergenic	30.0%
	ACTAAAATCAATTCCAATCC+CGG	+	chr8_4:5451526-5451545	Msa1309050:intron	30.0%
	AACTCTATTTCCCAAATTGA+TGG	-	chr8_4:5451703-5451722	None:intergenic	30.0%
!!	TTGGGAAATAGAGTTTTGAT+TGG	+	chr8_4:5451708-5451727	Msa1309050:CDS	30.0%
!!!	TGGGAAATAGAGTTTTGATT+GGG	+	chr8_4:5451709-5451728	Msa1309050:CDS	30.0%
!!!	TTGATGATTTTGGTTCAACA+AGG	+	chr8_4:5451828-5451847	Msa1309050:intron	30.0%
!	TCTACATATCTTTCCTTTTG+AGG	+	chr8_4:5451856-5451875	Msa1309050:intron	30.0%
!	TTGGTTTAGAATACCTCAAA+AGG	-	chr8_4:5451872-5451891	None:intergenic	30.0%
!!!	TTTGTTTTGTAGGTTTCACT+TGG	+	chr8_4:5451972-5451991	Msa1309050:intron	30.0%
	CTCGAAGACAGAAAAAATAT+TGG	+	chr8_4:5452317-5452336	Msa1309050:intron	30.0%
	GATTTAGTACAGAACAAACA+TGG	-	chr8_4:5452765-5452784	None:intergenic	30.0%
!	AAATCATTTTGAATGCCTCA+AGG	+	chr8_4:5452812-5452831	Msa1309050:intron	30.0%
	CTATTGTTAAGATAACCTTG+AGG	-	chr8_4:5452830-5452849	None:intergenic	30.0%
	ATGATGTGATCTTAGACATA+AGG	-	chr8_4:5453238-5453257	None:intergenic	30.0%
!	TATGTCTAAGATCACATCAT+TGG	+	chr8_4:5453238-5453257	Msa1309050:intron	30.0%
!!!	GCGTTTTGTATTTAGCTTTA+TGG	+	chr8_4:5453325-5453344	Msa1309050:intron	30.0%
!	CTTTATGGTGTTTTCTTGTA+TGG	+	chr8_4:5453340-5453359	Msa1309050:intron	30.0%
!!!	CGGTTTGTGGTTTTTTTTTT+TGG	+	chr8_4:5453580-5453599	Msa1309050:intron	30.0%
	ATGTATCGCTGTCTAAATTA+CGG	+	chr8_4:5453678-5453697	Msa1309050:intron	30.0%
	TGCACTAAATATGGTATGTT+TGG	+	chr8_4:5453792-5453811	Msa1309050:intron	30.0%
	AACAATGCTTCATAGTAGTT+TGG	-	chr8_4:5453866-5453885	None:intergenic	30.0%
!	ATCGATGTAGTGATTTTTCT+TGG	-	chr8_4:5454167-5454186	None:intergenic	30.0%
!	AAAGTGTCACAACTTTAACT+TGG	+	chr8_4:5454240-5454259	Msa1309050:intron	30.0%
!!	CAACTTTAACTTGGTGAATT+TGG	+	chr8_4:5454249-5454268	Msa1309050:intron	30.0%
	TTCAAGGACTAACAATTTCA+TGG	-	chr8_4:5454384-5454403	None:intergenic	30.0%
!	GGTTGACCTTAAATTTTTCA+AGG	-	chr8_4:5454400-5454419	None:intergenic	30.0%
!	AATTTAAGGTCAACCATTCA+AGG	+	chr8_4:5454405-5454424	Msa1309050:intron	30.0%
	TCTTTCATTGAAGTTTCCAT+GGG	-	chr8_4:5454512-5454531	None:intergenic	30.0%
	ATCTTTCATTGAAGTTTCCA+TGG	-	chr8_4:5454513-5454532	None:intergenic	30.0%
	CAGTTTATGTTTAACCCAAA+TGG	+	chr8_4:5454594-5454613	Msa1309050:intron	30.0%
	CATTCAGTAAATGATGTATG+TGG	-	chr8_4:5454967-5454986	None:intergenic	30.0%
	TTTAAATGAAATACTCCCTC+CGG	-	chr8_4:5455024-5455043	None:intergenic	30.0%
	TAAATTCAACATGGAGTAGT+TGG	+	chr8_4:5455127-5455146	Msa1309050:intron	30.0%
	GACATTGACATAGTAAACAA+AGG	+	chr8_4:5455151-5455170	Msa1309050:intron	30.0%
	AAATCAGTTGCAAATGCATA+AGG	-	chr8_4:5455369-5455388	None:intergenic	30.0%
	TCTGGAAAAACTTTCAAAGT+TGG	+	chr8_4:5455444-5455463	Msa1309050:CDS	30.0%
!!	TTCAAAGTTGGTGATAACTT+AGG	+	chr8_4:5455456-5455475	Msa1309050:CDS	30.0%
	TAGGTATGTATACATTCTGA+CGG	+	chr8_4:5455475-5455494	Msa1309050:intron	30.0%
!!!	AATGGTGTTAGTCTTTTGAT+GGG	+	chr8_4:5456073-5456092	Msa1309050:CDS	30.0%
!!	CTTTGTTTCAGCAATGATTT+TGG	+	chr8_4:5456102-5456121	Msa1309050:CDS	30.0%
!!!	AATTCCTTCCTTTTGTTGTT+TGG	+	chr8_4:5456286-5456305	Msa1309050:three_prime_UTR	30.0%
	TTGGTTGCCAATAATTGAAA+TGG	+	chr8_4:5456305-5456324	Msa1309050:three_prime_UTR	30.0%
!	TTTCTTCAATAAGTTGGCAG+AGG	+	chr8_4:5451118-5451137	Msa1309050:five_prime_UTR	35.0%
!!	GAAGAAGCTTTGTTGATGAA+GGG	-	chr8_4:5451360-5451379	None:intergenic	35.0%
!	TGATGTTTGAAATTCAGCGA+GGG	-	chr8_4:5451382-5451401	None:intergenic	35.0%
!	TTGATGTTTGAAATTCAGCG+AGG	-	chr8_4:5451383-5451402	None:intergenic	35.0%
!!	AAAGGCATCACAATTGAAAC+CGG	-	chr8_4:5451548-5451567	None:intergenic	35.0%
	TCGGCTTCTCAATCATAAAA+TGG	-	chr8_4:5451579-5451598	None:intergenic	35.0%
	ATCATTCCATAGCCAATCAA+CGG	-	chr8_4:5451666-5451685	None:intergenic	35.0%
!	CTATTGTTGTTCACTGTTTG+AGG	+	chr8_4:5451766-5451785	Msa1309050:intron	35.0%
	AAGCAAAGTTGCGTTAAAGT+TGG	-	chr8_4:5451913-5451932	None:intergenic	35.0%
	ATGCTTTGATACATCAGTGT+TGG	+	chr8_4:5452361-5452380	Msa1309050:intron	35.0%
	ATGCACTCATTGCTTGATAA+TGG	-	chr8_4:5453098-5453117	None:intergenic	35.0%
!!	CAGTTGGTAATATCACAAAG+TGG	-	chr8_4:5453161-5453180	None:intergenic	35.0%
	AAGCAAACATTGCTTACAGT+TGG	-	chr8_4:5453177-5453196	None:intergenic	35.0%
	ATGATAACAGATTGCGCATA+AGG	-	chr8_4:5453559-5453578	None:intergenic	35.0%
	ATGCGCAATCTGTTATCATT+CGG	+	chr8_4:5453560-5453579	Msa1309050:intron	35.0%
!	ATCTGTTATCATTCGGTTTG+TGG	+	chr8_4:5453567-5453586	Msa1309050:intron	35.0%
	TGGTGATTTCACCTAAATTG+CGG	-	chr8_4:5453630-5453649	None:intergenic	35.0%
	TTCTATCCATCCATTCACAT+TGG	-	chr8_4:5453708-5453727	None:intergenic	35.0%
	TATTCTACAGTCACACAACT+TGG	-	chr8_4:5454102-5454121	None:intergenic	35.0%
!	GATGTAGTGATTTTTCTTGG+AGG	-	chr8_4:5454164-5454183	None:intergenic	35.0%
!	TTCCAGCAATTTCTAGATGT+TGG	+	chr8_4:5454194-5454213	Msa1309050:intron	35.0%
	AACCAACATCTAGAAATTGC+TGG	-	chr8_4:5454199-5454218	None:intergenic	35.0%
	TGCAAGCGAAAGAAAATTAG+TGG	-	chr8_4:5454290-5454309	None:intergenic	35.0%
!	TGCATTCATTCAAGGTATTC+TGG	+	chr8_4:5454355-5454374	Msa1309050:intron	35.0%
!	ATTCATTCAAGGTATTCTGG+TGG	+	chr8_4:5454358-5454377	Msa1309050:intron	35.0%
	TTTGAGCTGCTACAATGTTA+TGG	+	chr8_4:5454448-5454467	Msa1309050:intron	35.0%
!	GAGTGCACATTCACATTTTT+AGG	+	chr8_4:5454470-5454489	Msa1309050:intron	35.0%
	TAATTACTCAGACTCATGTC+CGG	-	chr8_4:5454649-5454668	None:intergenic	35.0%
	TTTATTGAATGTACTCCCTC+CGG	+	chr8_4:5454872-5454891	Msa1309050:intron	35.0%
	AAATGATGAGATTCAGGTAG+AGG	-	chr8_4:5455064-5455083	None:intergenic	35.0%
	ATGATAGATGAGGTTATGGA+AGG	-	chr8_4:5455202-5455221	None:intergenic	35.0%
	TCCATAACCTCATCTATCAT+TGG	+	chr8_4:5455202-5455221	Msa1309050:intron	35.0%
	AGTTGCAAATGCATAAGGAA+AGG	-	chr8_4:5455364-5455383	None:intergenic	35.0%
!	TTTGCAACTGATTTCACTGT+TGG	+	chr8_4:5455375-5455394	Msa1309050:CDS	35.0%
!	AAGGAGTTGATTACACCAAA+TGG	+	chr8_4:5455418-5455437	Msa1309050:CDS	35.0%
!	AGGAGTTGATTACACCAAAT+GGG	+	chr8_4:5455419-5455438	Msa1309050:CDS	35.0%
	TCCATCAGCATAGTTCTTTA+TGG	-	chr8_4:5455789-5455808	None:intergenic	35.0%
	CTATGCTGATGGAAATTCTA+AGG	+	chr8_4:5455796-5455815	Msa1309050:CDS	35.0%
!	TTGATGGAGATTTTGTTGGT+GGG	-	chr8_4:5455952-5455971	None:intergenic	35.0%
	CATCAAAAGACTAACACCAT+TGG	-	chr8_4:5456074-5456093	None:intergenic	35.0%
!!!	CAATGGTGTTAGTCTTTTGA+TGG	+	chr8_4:5456072-5456091	Msa1309050:CDS	35.0%
!!!	AGCAATGATTTTGGGTTTGA+TGG	+	chr8_4:5456111-5456130	Msa1309050:CDS	35.0%
!!!	GCAATGATTTTGGGTTTGAT+GGG	+	chr8_4:5456112-5456131	Msa1309050:CDS	35.0%
	ATTGGCAACCAAACAACAAA+AGG	-	chr8_4:5456297-5456316	None:intergenic	35.0%
	TGACTTTCAAACAAGTGACC+TGG	+	chr8_4:5451156-5451175	Msa1309050:five_prime_UTR	40.0%
	GACTTTCAAACAAGTGACCT+GGG	+	chr8_4:5451157-5451176	Msa1309050:five_prime_UTR	40.0%
!	TGATTTGAAATAGGAGACCC+AGG	-	chr8_4:5451177-5451196	None:intergenic	40.0%
	GATAAAGAACCAGATGGTGA+AGG	-	chr8_4:5451231-5451250	None:intergenic	40.0%
!!	GGAAGAAGCTTTGTTGATGA+AGG	-	chr8_4:5451361-5451380	None:intergenic	40.0%
	ATCACAATTGAAACCGGGAT+TGG	-	chr8_4:5451542-5451561	None:intergenic	40.0%
!	AAGGCATCACAATTGAAACC+GGG	-	chr8_4:5451547-5451566	None:intergenic	40.0%
	AACAACCAATCTCCGTTGAT+TGG	+	chr8_4:5451651-5451670	Msa1309050:five_prime_UTR	40.0%
	TGTGTCTGTTTATGCACTCA+TGG	+	chr8_4:5452559-5452578	Msa1309050:intron	40.0%
!!	TATTCTGAAATAGGCGGCAT+AGG	-	chr8_4:5453008-5453027	None:intergenic	40.0%
	TTACATGAAGATACCGCTGA+AGG	+	chr8_4:5453122-5453141	Msa1309050:intron	40.0%
	TTGAACATAGCTTTCGAGCA+CGG	+	chr8_4:5453403-5453422	Msa1309050:intron	40.0%
!	TCTCTACTGTGTTGACACTA+TGG	-	chr8_4:5453526-5453545	None:intergenic	40.0%
	TGCTTATGTTGCCGCAATTT+AGG	+	chr8_4:5453616-5453635	Msa1309050:intron	40.0%
	ATCGCTGTCTAAATTACGGT+TGG	+	chr8_4:5453682-5453701	Msa1309050:intron	40.0%
!	GCTTCATAGTAGTTTGGTGA+GGG	-	chr8_4:5453860-5453879	None:intergenic	40.0%
!	TGCTTCATAGTAGTTTGGTG+AGG	-	chr8_4:5453861-5453880	None:intergenic	40.0%
	GTGCAGAGAATTGTTACTGA+TGG	-	chr8_4:5454126-5454145	None:intergenic	40.0%
	CTAGAAATTGCTGGAATGAG+AGG	-	chr8_4:5454190-5454209	None:intergenic	40.0%
!	AGTAAAGTTTGGAATGCCCA+TGG	+	chr8_4:5454493-5454512	Msa1309050:intron	40.0%
!	TTCGGTTTGATTACAGCTAG+AGG	-	chr8_4:5454567-5454586	None:intergenic	40.0%
	ACGGCTAACTGTACTATAAG+TGG	-	chr8_4:5454721-5454740	None:intergenic	40.0%
	TGCTTATAATAGTGACCGGA+GGG	-	chr8_4:5455006-5455025	None:intergenic	40.0%
	TTGCTTATAATAGTGACCGG+AGG	-	chr8_4:5455005-5455024	None:intergenic	40.0%
	TTGCTTATAATAGTGACCGG+AGG	+	chr8_4:5455005-5455024	Msa1309050:intron	40.0%
	TGCTTATAATAGTGACCGGA+GGG	+	chr8_4:5455006-5455025	Msa1309050:intron	40.0%
	ATTCAGGTAGAGGCAACATA+TGG	-	chr8_4:5455054-5455073	None:intergenic	40.0%
	ACGGGTAAATGATGAGATTC+AGG	-	chr8_4:5455070-5455089	None:intergenic	40.0%
	TAGATGAGGTTATGGAAGGA+GGG	-	chr8_4:5455198-5455217	None:intergenic	40.0%
	ATAGATGAGGTTATGGAAGG+AGG	-	chr8_4:5455199-5455218	None:intergenic	40.0%
	GCCAATGATAGATGAGGTTA+TGG	-	chr8_4:5455206-5455225	None:intergenic	40.0%
	ACTTGTGCCAATGATAGATG+AGG	-	chr8_4:5455212-5455231	None:intergenic	40.0%
!	AGTTTTTCCAGAAGCCCATT+TGG	-	chr8_4:5455436-5455455	None:intergenic	40.0%
	TTTCATCAACCTCATCCACT+TGG	-	chr8_4:5455736-5455755	None:intergenic	40.0%
	GCCATAAAGAACTATGCTGA+TGG	+	chr8_4:5455785-5455804	Msa1309050:CDS	40.0%
!!	AAGGTTGCATTGACTAAAGC+AGG	+	chr8_4:5455815-5455834	Msa1309050:CDS	40.0%
	TAGTACAATGACCTGGAGTA+GGG	-	chr8_4:5455859-5455878	None:intergenic	40.0%
!	TGATGGAGATTTTGTTGGTG+GGG	-	chr8_4:5455951-5455970	None:intergenic	40.0%
!!	GTTGATGGAGATTTTGTTGG+TGG	-	chr8_4:5455953-5455972	None:intergenic	40.0%
!!!	GTGGTTGATGGAGATTTTGT+TGG	-	chr8_4:5455956-5455975	None:intergenic	40.0%
	GAAGTTGAGTTTGTCTCTGA+AGG	-	chr8_4:5456013-5456032	None:intergenic	40.0%
	CTAACAGCTCCATTATCCTT+TGG	-	chr8_4:5456046-5456065	None:intergenic	40.0%
!!!	ATTTTGGGTTTGATGGGCTA+GGG	+	chr8_4:5456118-5456137	Msa1309050:CDS	40.0%
	GCAACCAAACAACAAAAGGA+AGG	-	chr8_4:5456293-5456312	None:intergenic	40.0%
	AGAACCAGATGGTGAAGGAA+TGG	-	chr8_4:5451226-5451245	None:intergenic	45.0%
!	TTTGTTGATGAAGGGTGGTG+AGG	-	chr8_4:5451352-5451371	None:intergenic	45.0%
!!	GAAGCTTTGTTGATGAAGGG+TGG	-	chr8_4:5451357-5451376	None:intergenic	45.0%
	TTATGATTGAGAAGCCGACG+TGG	+	chr8_4:5451581-5451600	Msa1309050:intron	45.0%
	CATAGCCAATCAACGGAGAT+TGG	-	chr8_4:5451659-5451678	None:intergenic	45.0%
!	CAATCTCCGTTGATTGGCTA+TGG	+	chr8_4:5451657-5451676	Msa1309050:five_prime_UTR	45.0%
	TGAGCCACTCAAAATACGCT+CGG	-	chr8_4:5452864-5452883	None:intergenic	45.0%
!!	AGCGTATTTTGAGTGGCTCA+AGG	+	chr8_4:5452864-5452883	Msa1309050:intron	45.0%
!	CGTTCTGTTACTTCCTTCAG+CGG	-	chr8_4:5453138-5453157	None:intergenic	45.0%
!	TTACGGTTGGCCAATGTGAA+TGG	+	chr8_4:5453695-5453714	Msa1309050:intron	45.0%
	AGATGAGGTTATGGAAGGAG+GGG	-	chr8_4:5455197-5455216	None:intergenic	45.0%
	AACTCCTTGATTCCATCCAC+TGG	-	chr8_4:5455406-5455425	None:intergenic	45.0%
!	GATTACACCAAATGGGCTTC+TGG	+	chr8_4:5455426-5455445	Msa1309050:CDS	45.0%
	TACTTCATATGCCCTACTCC+AGG	+	chr8_4:5455845-5455864	Msa1309050:CDS	45.0%
	CTAGTACAATGACCTGGAGT+AGG	-	chr8_4:5455860-5455879	None:intergenic	45.0%
	CCAGTACTAGTACAATGACC+TGG	-	chr8_4:5455866-5455885	None:intergenic	45.0%
	CCAGGTCATTGTACTAGTAC+TGG	+	chr8_4:5455863-5455882	Msa1309050:CDS	45.0%
!	GGTCATTGTACTAGTACTGG+TGG	+	chr8_4:5455866-5455885	Msa1309050:CDS	45.0%
!!	ATGGAGATTTTGTTGGTGGG+GGG	-	chr8_4:5455949-5455968	None:intergenic	45.0%
!!	GATGGAGATTTTGTTGGTGG+GGG	-	chr8_4:5455950-5455969	None:intergenic	45.0%
!!	GTAGTTGATGGTGTGGTTGA+TGG	-	chr8_4:5455968-5455987	None:intergenic	45.0%
!!	TGATGGAGTAGTTGATGGTG+TGG	-	chr8_4:5455975-5455994	None:intergenic	45.0%
!!	GTGGTTGATGGAGTAGTTGA+TGG	-	chr8_4:5455980-5455999	None:intergenic	45.0%
!	TTTGTCTCTGAAGGAGCTGA+TGG	-	chr8_4:5456004-5456023	None:intergenic	45.0%
	AACTTCTCCATCACCACCAA+AGG	+	chr8_4:5456027-5456046	Msa1309050:CDS	45.0%
!!	CCATTATCCTTTGGTGGTGA+TGG	-	chr8_4:5456037-5456056	None:intergenic	45.0%
	CCATCACCACCAAAGGATAA+TGG	+	chr8_4:5456034-5456053	Msa1309050:CDS	45.0%
	ACAGCTCCATTATCCTTTGG+TGG	-	chr8_4:5456043-5456062	None:intergenic	45.0%
	GGAGCTGTTAGTGTTTCCAA+TGG	+	chr8_4:5456055-5456074	Msa1309050:CDS	45.0%
!!!	GATTTTGGGTTTGATGGGCT+AGG	+	chr8_4:5456117-5456136	Msa1309050:CDS	45.0%
	AAGGAATGGAGGAGGAGGTT+TGG	-	chr8_4:5451212-5451231	None:intergenic	50.0%
	AGATGGTGAAGGAATGGAGG+AGG	-	chr8_4:5451220-5451239	None:intergenic	50.0%
	TCCTCCATTCCTTCACCATC+TGG	+	chr8_4:5451219-5451238	Msa1309050:five_prime_UTR	50.0%
	ACCAGATGGTGAAGGAATGG+AGG	-	chr8_4:5451223-5451242	None:intergenic	50.0%
!!	TGATGGTGGTGATGGAAGGA+CGG	-	chr8_4:5451310-5451329	None:intergenic	50.0%
!!	ATGGTGATGGTGGTGATGGA+AGG	-	chr8_4:5451314-5451333	None:intergenic	50.0%
	ACGAGAGAAGACGAAGCAGT+GGG	+	chr8_4:5451611-5451630	Msa1309050:five_prime_UTR	50.0%
	GGTTTCACTTGGTTGCTGCT+TGG	+	chr8_4:5451983-5452002	Msa1309050:intron	50.0%
!!	CATGCCGAGCGTATTTTGAG+TGG	+	chr8_4:5452857-5452876	Msa1309050:intron	50.0%
!	GGTTGGCCAATGTGAATGGA+TGG	+	chr8_4:5453699-5453718	Msa1309050:intron	50.0%
	CATTTACCCGTGCGATAGCA+CGG	+	chr8_4:5455079-5455098	Msa1309050:intron	50.0%
	ATTTACCCGTGCGATAGCAC+GGG	+	chr8_4:5455080-5455099	Msa1309050:intron	50.0%
!!	TTCACTGTTGGTGATGCCAG+TGG	+	chr8_4:5455387-5455406	Msa1309050:CDS	50.0%
	GATGCCAGTGGATGGAATCA+AGG	+	chr8_4:5455399-5455418	Msa1309050:CDS	50.0%
	ATATGGCTCCTTCCACCAAG+TGG	+	chr8_4:5455718-5455737	Msa1309050:CDS	50.0%
!	AACCTCATCCACTTGGTGGA+AGG	-	chr8_4:5455729-5455748	None:intergenic	50.0%
	CATCAACCTCATCCACTTGG+TGG	-	chr8_4:5455733-5455752	None:intergenic	50.0%
!!	TGGAGATTTTGTTGGTGGGG+GGG	-	chr8_4:5455948-5455967	None:intergenic	50.0%
	TGGTGAAGGAATGGAGGAGG+AGG	-	chr8_4:5451217-5451236	None:intergenic	55.0%
	GAGAGGGTGGATGGTTTGAG+AGG	-	chr8_4:5451268-5451287	None:intergenic	55.0%
!!	GGTGATGGTGATGGTGGTGA+TGG	-	chr8_4:5451318-5451337	None:intergenic	55.0%
!!	GACGATGGTGATGGTGATGG+TGG	-	chr8_4:5451324-5451343	None:intergenic	55.0%
!!	GAGGACGATGGTGATGGTGA+TGG	-	chr8_4:5451327-5451346	None:intergenic	55.0%
	GAGGAAGAGGACGATGGTGA+TGG	-	chr8_4:5451333-5451352	None:intergenic	55.0%
	GATGAAGGGTGGTGAGGAAG+AGG	-	chr8_4:5451346-5451365	None:intergenic	55.0%
	TCCATCGCAAGTCATCGCGT+CGG	+	chr8_4:5451422-5451441	Msa1309050:five_prime_UTR	55.0%
!	GTCATCGCGTCGGCAATTAC+AGG	+	chr8_4:5451432-5451451	Msa1309050:five_prime_UTR	55.0%
	CTCTCGTGGATCTTCCACGT+CGG	-	chr8_4:5451598-5451617	None:intergenic	55.0%
	CACTGCTTCGTCTTCTCTCG+TGG	-	chr8_4:5451612-5451631	None:intergenic	55.0%
	CACGAGAGAAGACGAAGCAG+TGG	+	chr8_4:5451610-5451629	Msa1309050:five_prime_UTR	55.0%
	AGAGAAGACGAAGCAGTGGG+AGG	+	chr8_4:5451614-5451633	Msa1309050:five_prime_UTR	55.0%
!!	CTGTTGGTGATGCCAGTGGA+TGG	+	chr8_4:5455391-5455410	Msa1309050:CDS	55.0%
	CTCCTTCCACCAAGTGGATG+AGG	+	chr8_4:5455724-5455743	Msa1309050:CDS	55.0%
!	GGAGCTGATGGTGTGGTTGA+TGG	-	chr8_4:5455992-5456011	None:intergenic	55.0%
!	CTCTGAAGGAGCTGATGGTG+TGG	-	chr8_4:5455999-5456018	None:intergenic	55.0%
	GGAGGAGGAGGTTTGGCATG+TGG	-	chr8_4:5451205-5451224	None:intergenic	60.0%
!!	GGTGGTGATGGAAGGACGGT+GGG	-	chr8_4:5451306-5451325	None:intergenic	60.0%
!!	TGGTGGTGATGGAAGGACGG+TGG	-	chr8_4:5451307-5451326	None:intergenic	60.0%
	GGTGGTGAGGAAGAGGACGA+TGG	-	chr8_4:5451339-5451358	None:intergenic	60.0%
	AGCACCACCCCAACTCCAAG+TGG	+	chr8_4:5455920-5455939	Msa1309050:CDS	60.0%
!!	ATGGAAGGACGGTGGGCTGG+TGG	-	chr8_4:5451299-5451318	None:intergenic	65.0%
!	GTGATGGAAGGACGGTGGGC+TGG	-	chr8_4:5451302-5451321	None:intergenic	65.0%
!	GCCGACGCGATGACTTGCGA+TGG	-	chr8_4:5451426-5451445	None:intergenic	65.0%
	ACCCGTGCGATAGCACGGGA+CGG	+	chr8_4:5455084-5455103	Msa1309050:intron	65.0%
!!	GGTGCCACTTGGAGTTGGGG+TGG	-	chr8_4:5455927-5455946	None:intergenic	65.0%
!!	GGGGGTGCCACTTGGAGTTG+GGG	-	chr8_4:5455930-5455949	None:intergenic	65.0%
!!	GGGGGGTGCCACTTGGAGTT+GGG	-	chr8_4:5455931-5455950	None:intergenic	65.0%
	GCGGTGGAGGAGAGGGTGGA+TGG	-	chr8_4:5451277-5451296	None:intergenic	70.0%
!!	GCTGGTGGCGGTGGAGGAGA+GGG	-	chr8_4:5451284-5451303	None:intergenic	70.0%
!!	GAAGGACGGTGGGCTGGTGG+CGG	-	chr8_4:5451296-5451315	None:intergenic	70.0%
	GCCGTCCCGTGCTATCGCAC+GGG	-	chr8_4:5455088-5455107	None:intergenic	70.0%
!!	GGGGGGGTGCCACTTGGAGT+TGG	-	chr8_4:5455932-5455951	None:intergenic	70.0%
!!	GTTGGTGGGGGGGTGCCACT+TGG	-	chr8_4:5455938-5455957	None:intergenic	70.0%
!	GGTGGCGGTGGAGGAGAGGG+TGG	-	chr8_4:5451281-5451300	None:intergenic	75.0%
!!	GGCTGGTGGCGGTGGAGGAG+AGG	-	chr8_4:5451285-5451304	None:intergenic	75.0%
!!!	CGGTGGGCTGGTGGCGGTGG+AGG	-	chr8_4:5451290-5451309	None:intergenic	80.0%
!!!	GGACGGTGGGCTGGTGGCGG+TGG	-	chr8_4:5451293-5451312	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	5451108	5456361	5451108	ID=Msa1309050;Name=Msa1309050
chr8_4	mRNA	5451108	5456361	5451108	ID=Msa1309050-mRNA-1;Parent=Msa1309050;Name=Msa1309050-mRNA-1;_AED=0.25;_eAED=0.26;_QI=435\|0.16\|0.28\|1\|0.33\|0.42\|7\|223\|250
chr8_4	exon	5451108	5451453	5451108	ID=Msa1309050-mRNA-1:exon:1732;Parent=Msa1309050-mRNA-1
chr8_4	exon	5451592	5451770	5451592	ID=Msa1309050-mRNA-1:exon:1733;Parent=Msa1309050-mRNA-1
chr8_4	exon	5451984	5452040	5451984	ID=Msa1309050-mRNA-1:exon:1734;Parent=Msa1309050-mRNA-1
chr8_4	exon	5452733	5452741	5452733	ID=Msa1309050-mRNA-1:exon:1735;Parent=Msa1309050-mRNA-1
chr8_4	exon	5453340	5453346	5453340	ID=Msa1309050-mRNA-1:exon:1736;Parent=Msa1309050-mRNA-1
chr8_4	exon	5455316	5455477	5455316	ID=Msa1309050-mRNA-1:exon:1737;Parent=Msa1309050-mRNA-1
chr8_4	exon	5455711	5456361	5455711	ID=Msa1309050-mRNA-1:exon:1738;Parent=Msa1309050-mRNA-1
chr8_4	five_prime_UTR	5451108	5451453	5451108	ID=Msa1309050-mRNA-1:five_prime_utr;Parent=Msa1309050-mRNA-1
chr8_4	five_prime_UTR	5451592	5451680	5451592	ID=Msa1309050-mRNA-1:five_prime_utr;Parent=Msa1309050-mRNA-1
chr8_4	CDS	5451681	5451770	5451681	ID=Msa1309050-mRNA-1:cds;Parent=Msa1309050-mRNA-1
chr8_4	CDS	5451984	5452040	5451984	ID=Msa1309050-mRNA-1:cds;Parent=Msa1309050-mRNA-1
chr8_4	CDS	5452733	5452741	5452733	ID=Msa1309050-mRNA-1:cds;Parent=Msa1309050-mRNA-1
chr8_4	CDS	5453340	5453346	5453340	ID=Msa1309050-mRNA-1:cds;Parent=Msa1309050-mRNA-1
chr8_4	CDS	5455316	5455477	5455316	ID=Msa1309050-mRNA-1:cds;Parent=Msa1309050-mRNA-1
chr8_4	CDS	5455711	5456138	5455711	ID=Msa1309050-mRNA-1:cds;Parent=Msa1309050-mRNA-1
chr8_4	three_prime_UTR	5456139	5456361	5456139	ID=Msa1309050-mRNA-1:three_prime_utr;Parent=Msa1309050-mRNA-1

Gene Sequence

>Msa1309050

ATGATACAAAAAATTTCTACCATCAATTTGGGAAATAGAGTTTTGATTGGGTATTTTTTATCATTGTTCTTTCGCTTCAGCTATGCTATTGTTTCACTTGGTTGCTGCTTGGTGCTGAGTGTAAAAGAGAAGAAATTATTACTCTATGTATCGATACTTTATGGCATGAATACATCAATGATAGCTTCTTTCTTTGTTCTATTGTTGGCCTTTCCTTATGCATTTGCAACTGATTTCACTGTTGGTGATGCCAGTGGATGGAATCAAGGAGTTGATTACACCAAATGGGCTTCTGGAAAAACTTTCAAAGTTGGTGATAACTTAGTGTTCAAATATGGCTCCTTCCACCAAGTGGATGAGGTTGATGAAAGTGACTACAAAAGCTGCACTTCAAGCAACGCCATAAAGAACTATGCTGATGGAAATTCTAAGGTTGCATTGACTAAAGCAGGAAAAATTTACTTCATATGCCCTACTCCAGGTCATTGTACTAGTACTGGTGGCATGAAGCTTGAAGTCAATGTTGTTGCTGCTAGCACCACCCCAACTCCAAGTGGCACCCCCCCACCAACAAAATCTCCATCAACCACACCATCAACTACTCCATCAACCACACCATCAGCTCCTTCAGAGACAAACTCAACTTCTCCATCACCACCAAAGGATAATGGAGCTGTTAGTGTTTCCAATGGTGTTAGTCTTTTGATGGGTTCTTTCTTTGTTTCAGCAATGATTTTGGGTTTGATGGGCTAG

Protein sequence

>Msa1309050

MIQKISTINLGNRVLIGYFLSLFFRFSYAIVSLGCCLVLSVKEKKLLLYVSILYGMNTSMIASFFVLLLAFPYAFATDFTVGDASGWNQGVDYTKWASGKTFKVGDNLVFKYGSFHQVDEVDESDYKSCTSSNAIKNYADGNSKVALTKAGKIYFICPTPGHCTSTGGMKLEVNVVAASTTPTPSGTPPPTKSPSTTPSTTPSTTPSAPSETNSTSPSPPKDNGAVSVSNGVSLLMGSFFVSAMILGLMG*