Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344330 | XP_013469983.1 | 93.805 | 113 | 7 | 0 | 1 | 113 | 1 | 113 | 1.21e-72 | 221 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344330 | A0A072VQS7 | 93.805 | 113 | 7 | 0 | 1 | 113 | 1 | 113 | 5.80e-73 | 221 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011920 | Msa1344330 | -0.806776 | 6.980984e-50 | -8.615850e-47 |
| Msa0037720 | Msa1344330 | 0.800797 | 1.217871e-48 | -8.615850e-47 |
| Msa0052780 | Msa1344330 | 0.818533 | 1.864973e-52 | -8.615850e-47 |
| Msa0076640 | Msa1344330 | 0.864779 | 9.512585e-65 | -8.615850e-47 |
| Msa0097570 | Msa1344330 | 0.814244 | 1.700053e-51 | -8.615850e-47 |
| Msa1344330 | Msa1349830 | 0.837665 | 4.545658e-57 | -8.615850e-47 |
| Msa1344330 | Msa1398120 | 0.809242 | 2.085003e-50 | -8.615850e-47 |
| Msa1344330 | Msa1401110 | 0.848653 | 5.344055e-60 | -8.615850e-47 |
| Msa1344330 | Msa1412410 | 0.809094 | 2.242554e-50 | -8.615850e-47 |
| Msa1344330 | Msa1422230 | 0.802716 | 4.915629e-49 | -8.615850e-47 |
| Msa1344330 | Msa1427860 | 0.810304 | 1.231875e-50 | -8.615850e-47 |
| Msa1344330 | Msa1443220 | 0.803362 | 3.614383e-49 | -8.615850e-47 |
| Msa1344330 | Msa1453600 | 0.822805 | 1.946563e-53 | -8.615850e-47 |
| Msa1344330 | Msa1459920 | 0.810331 | 1.215544e-50 | -8.615850e-47 |
| Msa1344330 | Msa1459930 | 0.811444 | 6.979182e-51 | -8.615850e-47 |
| Msa0614290 | Msa1344330 | 0.815718 | 8.007925e-52 | -8.615850e-47 |
| Msa0697510 | Msa1344330 | 0.801122 | 1.044969e-48 | -8.615850e-47 |
| Msa0744540 | Msa1344330 | 0.817346 | 3.459359e-52 | -8.615850e-47 |
| Msa0744560 | Msa1344330 | 0.822924 | 1.825997e-53 | -8.615850e-47 |
| Msa0746340 | Msa1344330 | 0.802496 | 5.458879e-49 | -8.615850e-47 |
| Msa0816630 | Msa1344330 | 0.829442 | 5.136074e-55 | -8.615850e-47 |
| Msa0826360 | Msa1344330 | 0.815495 | 8.977822e-52 | -8.615850e-47 |
| Msa0826370 | Msa1344330 | 0.827283 | 1.704432e-54 | -8.615850e-47 |
| Msa0826380 | Msa1344330 | 0.840047 | 1.100314e-57 | -8.615850e-47 |
| Msa0826450 | Msa1344330 | 0.833884 | 4.125319e-56 | -8.615850e-47 |
| Msa0129640 | Msa1344330 | 0.812002 | 5.276834e-51 | -8.615850e-47 |
| Msa0145430 | Msa1344330 | 0.810552 | 1.089289e-50 | -8.615850e-47 |
| Msa0147790 | Msa1344330 | 0.821389 | 4.143126e-53 | -8.615850e-47 |
| Msa0224210 | Msa1344330 | 0.855539 | 5.894802e-62 | -8.615850e-47 |
| Msa0235840 | Msa1344330 | 0.815692 | 8.113862e-52 | -8.615850e-47 |
| Msa0238710 | Msa1344330 | 0.809827 | 1.560895e-50 | -8.615850e-47 |
| Msa1228810 | Msa1344330 | 0.835669 | 1.467067e-56 | -8.615850e-47 |
| Msa1309050 | Msa1344330 | 0.840461 | 8.576664e-58 | -8.615850e-47 |
| Msa1322180 | Msa1344330 | 0.815863 | 7.432057e-52 | -8.615850e-47 |
| Msa1333700 | Msa1344330 | 0.803218 | 3.870887e-49 | -8.615850e-47 |
| Msa1344220 | Msa1344330 | 0.809825 | 1.562805e-50 | -8.615850e-47 |
| Msa0374290 | Msa1344330 | 0.838668 | 2.507772e-57 | -8.615850e-47 |
| Msa0385320 | Msa1344330 | 0.828263 | 9.909063e-55 | -8.615850e-47 |
| Msa0390790 | Msa1344330 | 0.860385 | 2.143506e-63 | -8.615850e-47 |
| Msa0402800 | Msa1344330 | 0.822941 | 1.809144e-53 | -8.615850e-47 |
| Msa0414720 | Msa1344330 | 0.832655 | 8.352958e-56 | -8.615850e-47 |
| Msa0449620 | Msa1344330 | 0.819120 | 1.372055e-52 | -8.615850e-47 |
| Msa0459630 | Msa1344330 | 0.831221 | 1.887895e-55 | -8.615850e-47 |
| Msa0480530 | Msa1344330 | 0.812710 | 3.696952e-51 | -8.615850e-47 |
| Msa0480550 | Msa1344330 | 0.824004 | 1.020983e-53 | -8.615850e-47 |
| Msa0481740 | Msa1344330 | 0.818199 | 2.220149e-52 | -8.615850e-47 |
| Msa0481770 | Msa1344330 | 0.822281 | 2.576110e-53 | -8.615850e-47 |
| Msa1080650 | Msa1344330 | 0.820820 | 5.603194e-53 | -8.615850e-47 |
| Msa0246140 | Msa1344330 | 0.833277 | 5.849046e-56 | -8.615850e-47 |
| Msa0262360 | Msa1344330 | 0.835983 | 1.221059e-56 | -8.615850e-47 |
| Msa0265480 | Msa1344330 | 0.821563 | 3.777037e-53 | -8.615850e-47 |
| Msa0284730 | Msa1344330 | 0.812257 | 4.643250e-51 | -8.615850e-47 |
| Msa0299340 | Msa1344330 | 0.808045 | 3.755451e-50 | -8.615850e-47 |
| Msa0300140 | Msa1344330 | 0.838879 | 2.212239e-57 | -8.615850e-47 |
| Msa0332360 | Msa1344330 | 0.850550 | 1.579817e-60 | -8.615850e-47 |
| Msa0340370 | Msa1344330 | 0.830513 | 2.815701e-55 | -8.615850e-47 |
| Msa1126440 | Msa1344330 | 0.819772 | 9.743674e-53 | -8.615850e-47 |
| Msa1157200 | Msa1344330 | 0.836767 | 7.714440e-57 | -8.615850e-47 |
| Msa1173150 | Msa1344330 | 0.815223 | 1.031991e-51 | -8.615850e-47 |
| Msa1183750 | Msa1344330 | 0.863840 | 1.867498e-64 | -8.615850e-47 |
| Msa1224510 | Msa1344330 | 0.806674 | 7.335765e-50 | -8.615850e-47 |
| Msa0488580 | Msa1344330 | 0.804661 | 1.940407e-49 | -8.615850e-47 |
| Msa0497860 | Msa1344330 | 0.819284 | 1.259431e-52 | -8.615850e-47 |
| Msa0506360 | Msa1344330 | 0.834809 | 2.417934e-56 | -8.615850e-47 |
| Msa0515540 | Msa1344330 | 0.825409 | 4.761063e-54 | -8.615850e-47 |
| Msa0515590 | Msa1344330 | 0.820940 | 5.258550e-53 | -8.615850e-47 |
| Msa0560280 | Msa1344330 | 0.818957 | 1.494089e-52 | -8.615850e-47 |
| Msa0573950 | Msa1344330 | 0.815541 | 8.768028e-52 | -8.615850e-47 |
| Msa0576560 | Msa1344330 | 0.818271 | 2.138500e-52 | -8.615850e-47 |
| Msa0863940 | Msa1344330 | 0.832331 | 1.004959e-55 | -8.615850e-47 |
| Msa0865810 | Msa1344330 | 0.834605 | 2.721670e-56 | -8.615850e-47 |
| Msa0865820 | Msa1344330 | 0.803562 | 3.285401e-49 | -8.615850e-47 |
| Msa0865830 | Msa1344330 | 0.823500 | 1.339793e-53 | -8.615850e-47 |
| Msa0876790 | Msa1344330 | 0.814507 | 1.487494e-51 | -8.615850e-47 |
| Msa0931420 | Msa1344330 | 0.811510 | 6.754121e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344330 | MtrunA17_Chr1g0207271 | 93.805 | 113 | 7 | 0 | 1 | 113 | 1 | 113 | 1.12e-76 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGTAGCTACCACATGTTAT+TGG | 0.297025 | tig0006806:+54614 | Msa1344330:CDS |
| ATGAGGTTTCTGCTTCTTGA+TGG | 0.301828 | tig0006806:-54547 | None:intergenic |
| ATTTGGTAATGATGGAAGTT+TGG | 0.326402 | tig0006806:+54719 | Msa1344330:CDS |
| TTGGAATCTGACTCATTAAC+AGG | 0.333936 | tig0006806:+54633 | Msa1344330:CDS |
| TTGATGGCTTGGATAAGATA+TGG | 0.362194 | tig0006806:-54531 | None:intergenic |
| GTTTCTGCTTCTTGATGGCT+TGG | 0.374881 | tig0006806:-54542 | None:intergenic |
| GAGCACAGATTTGGTAATGA+TGG | 0.406744 | tig0006806:+54711 | Msa1344330:CDS |
| AGCATAAGAAGCAGCAGCAC+TGG | 0.414803 | tig0006806:-54787 | None:intergenic |
| AGCTGTCACAATGATGCCTC+TGG | 0.415269 | tig0006806:-54748 | None:intergenic |
| GAGTTCTTAGAGCACAGATT+TGG | 0.435020 | tig0006806:+54702 | Msa1344330:CDS |
| CTCATCACCACAGCTTCAAC+AGG | 0.439540 | tig0006806:+54565 | Msa1344330:CDS |
| TGAAATTCTTTGAGGGTGGT+TGG | 0.465990 | tig0006806:-54812 | None:intergenic |
| TGCTCTAAGAACTCAGTTGT+GGG | 0.467329 | tig0006806:-54693 | None:intergenic |
| GAAATTCTTTGAGGGTGGTT+GGG | 0.475402 | tig0006806:-54811 | None:intergenic |
| ATGTTGTTGAAATTCTTTGA+GGG | 0.507140 | tig0006806:-54819 | None:intergenic |
| CTGTTGAAGCTGTGGTGATG+AGG | 0.531347 | tig0006806:-54564 | None:intergenic |
| GCTGTCACAATGATGCCTCT+GGG | 0.532008 | tig0006806:-54747 | None:intergenic |
| GCAGCACTGGCAGTGGTCGG+AGG | 0.539102 | tig0006806:-54774 | None:intergenic |
| GTGCTCTAAGAACTCAGTTG+TGG | 0.553458 | tig0006806:-54694 | None:intergenic |
| GCAGCAGCACTGGCAGTGGT+CGG | 0.562924 | tig0006806:-54777 | None:intergenic |
| AATGTTGTTGAAATTCTTTG+AGG | 0.567996 | tig0006806:-54820 | None:intergenic |
| AGAAGCAGCAGCACTGGCAG+TGG | 0.577609 | tig0006806:-54781 | None:intergenic |
| AAAGAAAGAGAAACAATGGA+AGG | 0.577762 | tig0006806:+54501 | None:intergenic |
| TTGTTGAAATTCTTTGAGGG+TGG | 0.597370 | tig0006806:-54816 | None:intergenic |
| AGACCTGGTTCTTCTATGAG+AGG | 0.610651 | tig0006806:-54658 | None:intergenic |
| AAAAGCTCCTGTTGAAGCTG+TGG | 0.619031 | tig0006806:-54572 | None:intergenic |
| GGAAGTTTGGTTAGCCCCAG+AGG | 0.621319 | tig0006806:+54732 | Msa1344330:CDS |
| CTGTCACAATGATGCCTCTG+GGG | 0.650846 | tig0006806:-54746 | None:intergenic |
| GATCCTCTCATAGAAGAACC+AGG | 0.662887 | tig0006806:+54655 | Msa1344330:CDS |
| GGGTTGCTGAAAATCAGACC+TGG | 0.666499 | tig0006806:-54673 | None:intergenic |
| AGTCAGATTCCAATAACATG+TGG | 0.679678 | tig0006806:-54623 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGTTGTTGAAATTCTTTGA+GGG | - | tig0006806:54822-54841 | None:intergenic | 25.0% |
| ! | AATGTTGTTGAAATTCTTTG+AGG | - | tig0006806:54823-54842 | None:intergenic | 25.0% |
| !! | ATTTGGTAATGATGGAAGTT+TGG | + | tig0006806:54719-54738 | Msa1344330:CDS | 30.0% |
| ! | TTGATGGCTTGGATAAGATA+TGG | - | tig0006806:54534-54553 | None:intergenic | 35.0% |
| AGTCAGATTCCAATAACATG+TGG | - | tig0006806:54626-54645 | None:intergenic | 35.0% | |
| TTGGAATCTGACTCATTAAC+AGG | + | tig0006806:54633-54652 | Msa1344330:CDS | 35.0% | |
| ! | TTGTTGAAATTCTTTGAGGG+TGG | - | tig0006806:54819-54838 | None:intergenic | 35.0% |
| ATGAGGTTTCTGCTTCTTGA+TGG | - | tig0006806:54550-54569 | None:intergenic | 40.0% | |
| ! | AACAGGAGCTTTTCTCATTC+TGG | + | tig0006806:54582-54601 | Msa1344330:CDS | 40.0% |
| ! | ACAGGAGCTTTTCTCATTCT+GGG | + | tig0006806:54583-54602 | Msa1344330:CDS | 40.0% |
| TCGTAGCTACCACATGTTAT+TGG | + | tig0006806:54614-54633 | Msa1344330:CDS | 40.0% | |
| !! | TGCTCTAAGAACTCAGTTGT+GGG | - | tig0006806:54696-54715 | None:intergenic | 40.0% |
| GAGTTCTTAGAGCACAGATT+TGG | + | tig0006806:54702-54721 | Msa1344330:CDS | 40.0% | |
| !! | GAGCACAGATTTGGTAATGA+TGG | + | tig0006806:54711-54730 | Msa1344330:CDS | 40.0% |
| ! | GAAATTCTTTGAGGGTGGTT+GGG | - | tig0006806:54814-54833 | None:intergenic | 40.0% |
| ! | TGAAATTCTTTGAGGGTGGT+TGG | - | tig0006806:54815-54834 | None:intergenic | 40.0% |
| ! | GTTTCTGCTTCTTGATGGCT+TGG | - | tig0006806:54545-54564 | None:intergenic | 45.0% |
| AAAAGCTCCTGTTGAAGCTG+TGG | - | tig0006806:54575-54594 | None:intergenic | 45.0% | |
| GATCCTCTCATAGAAGAACC+AGG | + | tig0006806:54655-54674 | Msa1344330:CDS | 45.0% | |
| ! | AGACCTGGTTCTTCTATGAG+AGG | - | tig0006806:54661-54680 | None:intergenic | 45.0% |
| !! | GTGCTCTAAGAACTCAGTTG+TGG | - | tig0006806:54697-54716 | None:intergenic | 45.0% |
| ! | CTGTTGAAGCTGTGGTGATG+AGG | - | tig0006806:54567-54586 | None:intergenic | 50.0% |
| CTCATCACCACAGCTTCAAC+AGG | + | tig0006806:54565-54584 | Msa1344330:CDS | 50.0% | |
| GGGTTGCTGAAAATCAGACC+TGG | - | tig0006806:54676-54695 | None:intergenic | 50.0% | |
| CTGTCACAATGATGCCTCTG+GGG | - | tig0006806:54749-54768 | None:intergenic | 50.0% | |
| GCTGTCACAATGATGCCTCT+GGG | - | tig0006806:54750-54769 | None:intergenic | 50.0% | |
| AGCTGTCACAATGATGCCTC+TGG | - | tig0006806:54751-54770 | None:intergenic | 50.0% | |
| AGCATAAGAAGCAGCAGCAC+TGG | - | tig0006806:54790-54809 | None:intergenic | 50.0% | |
| ! | GGAAGTTTGGTTAGCCCCAG+AGG | + | tig0006806:54732-54751 | Msa1344330:CDS | 55.0% |
| !! | AGAAGCAGCAGCACTGGCAG+TGG | - | tig0006806:54784-54803 | None:intergenic | 60.0% |
| !! | GCAGCAGCACTGGCAGTGGT+CGG | - | tig0006806:54780-54799 | None:intergenic | 65.0% |
| !! | GCAGCACTGGCAGTGGTCGG+AGG | - | tig0006806:54777-54796 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0006806 | gene | 54516 | 54857 | 54516 | ID=Msa1344330;Name=Msa1344330 |
| tig0006806 | mRNA | 54516 | 54857 | 54516 | ID=Msa1344330-mRNA-1;Parent=Msa1344330;Name=Msa1344330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|113 |
| tig0006806 | exon | 54516 | 54857 | 54516 | ID=Msa1344330-mRNA-1:exon:4440;Parent=Msa1344330-mRNA-1 |
| tig0006806 | CDS | 54516 | 54857 | 54516 | ID=Msa1344330-mRNA-1:cds;Parent=Msa1344330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
