AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0302290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302290 MtrunA17_Chr2g0328271 96.875 384 11 1 1 384 1 383 0.0 763
Msa0302290 MtrunA17_Chr4g0009841 62.824 347 126 2 37 383 34 377 1.18e-168 476
Msa0302290 MtrunA17_Chr7g0238701 59.178 365 140 4 23 383 4 363 2.51e-150 429
Msa0302290 MtrunA17_Chr6g0472501 59.412 340 122 3 1 338 1 326 2.14e-127 369
Msa0302290 MtrunA17_Chr1g0203681 53.191 329 135 3 16 339 5 319 2.59e-113 333
Msa0302290 MtrunA17_Chr2g0332941 55.636 275 114 3 44 312 30 302 5.47e-99 296
Msa0302290 MtrunA17_Chr3g0115331 39.257 377 216 5 13 382 9 379 2.35e-87 269
Msa0302290 MtrunA17_Chr2g0322601 39.500 200 94 5 142 340 1 174 1.15e-32 120
Msa0302290 MtrunA17_Chr2g0322611 48.913 92 47 0 99 190 2 93 3.72e-23 92.8
Msa0302290 MtrunA17_Chr8g0392201 38.542 96 43 2 225 320 28 107 1.36e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302290 AT2G29050.1 62.434 378 139 2 6 383 15 389 1.25e-175 494
Msa0302290 AT3G53780.2 61.318 349 127 2 35 383 54 394 1.10e-153 439
Msa0302290 AT1G63120.1 63.946 294 98 2 44 335 30 317 1.09e-128 372
Msa0302290 AT2G29050.2 58.104 327 106 3 6 304 15 338 4.91e-126 367
Msa0302290 AT5G07250.1 60.061 328 116 3 21 337 22 345 5.70e-125 364
Msa0302290 AT3G53780.1 61.957 276 99 1 108 383 1 270 3.57e-124 359
Msa0302290 AT1G12750.4 56.066 305 129 2 33 335 6 307 6.25e-122 355
Msa0302290 AT1G12750.7 56.066 305 129 2 33 335 6 307 6.25e-122 355
Msa0302290 AT1G12750.6 56.066 305 129 2 33 335 6 307 6.25e-122 355
Msa0302290 AT1G12750.3 56.066 305 129 2 33 335 6 307 6.25e-122 355
Msa0302290 AT1G12750.1 56.066 305 129 2 33 335 6 307 6.25e-122 355
Msa0302290 AT3G53780.3 62.547 267 92 2 35 301 54 312 2.33e-114 336
Msa0302290 AT1G52580.1 54.209 297 127 6 20 311 4 296 4.39e-97 292
Msa0302290 AT1G77860.2 44.083 338 184 4 48 382 49 384 1.12e-96 293
Msa0302290 AT4G23070.1 55.326 291 125 2 44 334 28 313 1.51e-93 283
Msa0302290 AT5G07250.2 49.695 328 103 4 21 337 22 298 1.55e-88 270
Msa0302290 AT1G77860.1 45.151 299 159 4 48 343 49 345 1.28e-84 261
Msa0302290 AT1G12750.5 60.326 184 71 1 33 214 6 189 2.42e-76 235
Msa0302290 AT1G12750.2 59.893 187 73 1 33 217 6 192 4.45e-76 234

Find 114 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAGGGTCTTGTTGGATTC+TGG 0.167687 2_3:-59087211 Msa0302290:five_prime_UTR
ATTCGGCTTGAACAAGAATT+TGG 0.175005 2_3:-59085697 Msa0302290:CDS
AAATGCTGTCGAATATTATT+AGG 0.205490 2_3:-59084101 Msa0302290:three_prime_UTR
TGATTCGTCCTCAGTTTAAA+TGG 0.238851 2_3:-59085052 Msa0302290:CDS
CTCTATGTGATATCTGGTTT+TGG 0.246364 2_3:-59085449 Msa0302290:CDS
GGATTTCTCTATGTGATATC+TGG 0.262897 2_3:-59085455 Msa0302290:CDS
AATGGAATGGAAGATGTATT+TGG 0.277815 2_3:-59084155 Msa0302290:three_prime_UTR
TCTCAGAGCTCTTCATTAAT+TGG 0.278075 2_3:-59085340 Msa0302290:CDS
TCTTTCCTATTACTCATTGC+AGG 0.286895 2_3:-59084939 Msa0302290:CDS
CTTACCCTGCAATGAGTAAT+AGG 0.292148 2_3:+59084934 None:intergenic
TTCGCTCATCTAGGAGGATT+TGG 0.297054 2_3:-59085104 Msa0302290:CDS
GGCGCCTTGTCTCTTGTATA+TGG 0.304879 2_3:-59085777 Msa0302290:CDS
CTTGAACAAGAATTTGGATT+TGG 0.310501 2_3:-59085691 Msa0302290:intron
ATCTGAAATCTTTGTTCAAA+AGG 0.315789 2_3:-59087058 Msa0302290:five_prime_UTR
CATCTAGGAGGATTTGGTTC+TGG 0.319719 2_3:-59085098 Msa0302290:CDS
AACAAATTCCTAACTTTGTC+AGG 0.325137 2_3:-59087228 Msa0302290:five_prime_UTR
TCACTCGTGATCGTGATTTC+AGG 0.355861 2_3:-59086915 Msa0302290:CDS
TTGTGTATATGATTCATTCT+TGG 0.360552 2_3:-59084337 Msa0302290:three_prime_UTR
TAACTTTGTCAGGGTCTTGT+TGG 0.364641 2_3:-59087218 Msa0302290:five_prime_UTR
CTTAAAGAAAACCCTCTCTT+TGG 0.366436 2_3:-59086739 Msa0302290:CDS
TGGCACCACCGTTGAGGTTA+AGG 0.367443 2_3:-59086986 Msa0302290:CDS
GGCATCTCAGTTGGTGCATC+TGG 0.372637 2_3:-59085392 Msa0302290:CDS
TTTGGTTCTGGCTTTCTGCT+TGG 0.373387 2_3:-59085086 Msa0302290:CDS
CTTGTATATGGTTGCATGGT+GGG 0.379600 2_3:-59085765 Msa0302290:CDS
TGTCCTTTCGCAGTTAGAAT+TGG 0.379685 2_3:-59085476 Msa0302290:intron
TTGGACGATATATGCCAATA+AGG 0.382911 2_3:-59085321 Msa0302290:intron
GGTGCATCTGGTGCATTGTT+TGG 0.382955 2_3:-59085380 Msa0302290:CDS
AGCCAACAACAGTCTAAATC+TGG 0.385356 2_3:-59085026 Msa0302290:CDS
ACCTTATCAATATGTGTTGT+GGG 0.394616 2_3:-59084967 Msa0302290:CDS
ATCCAGATTTAGACTGTTGT+TGG 0.400472 2_3:+59085024 None:intergenic
AACAAGACCCTGACAAAGTT+AGG 0.404982 2_3:+59087220 None:intergenic
AATGCTGTCGAATATTATTA+GGG 0.406993 2_3:-59084100 Msa0302290:three_prime_UTR
GTTGTGTTCCTACCTCAAAA+TGG 0.409054 2_3:-59084697 Msa0302290:CDS
TCTATGCAGACAACGGAATT+CGG 0.409614 2_3:-59084517 Msa0302290:intron
GAAATGGGTTTGAATCAGTT+AGG 0.421296 2_3:+59087144 None:intergenic
GTTGTAATAAATTTAGCTGT+TGG 0.441288 2_3:-59085149 Msa0302290:CDS
TTGTTCTCAGTCGGCAATGA+TGG 0.441972 2_3:-59084297 Msa0302290:three_prime_UTR
GTCTGCGTTATTCATACAAA+CGG 0.445629 2_3:-59085414 Msa0302290:CDS
TCTGAAATCTTTGTTCAAAA+GGG 0.452645 2_3:-59087057 Msa0302290:five_prime_UTR
GGGTCTTGTTGGATTCTGGC+TGG 0.453364 2_3:-59087207 Msa0302290:five_prime_UTR
TGTCCTGAGAATGCATTTCA+TGG 0.455723 2_3:-59086805 Msa0302290:CDS
TACCTTATCAATATGTGTTG+TGG 0.455869 2_3:-59084968 Msa0302290:CDS
AGTAATAAACTTATGATGGC+TGG 0.458120 2_3:+59084262 None:intergenic
GGTGCATTGTTTGGCTTACT+CGG 0.462230 2_3:-59085371 Msa0302290:CDS
TTTCAGGCTCTTTAAGCAAT+GGG 0.470163 2_3:-59086899 Msa0302290:CDS
GTTGACAATTTCGCTCATCT+AGG 0.472592 2_3:-59085113 Msa0302290:CDS
ACACATGCAAAATTTCTTGA+AGG 0.474975 2_3:-59084376 Msa0302290:three_prime_UTR
GTCTCTTGTATATGGTTGCA+TGG 0.482616 2_3:-59085769 Msa0302290:CDS
TAATAGTAATAAACTTATGA+TGG 0.486847 2_3:+59084258 None:intergenic
AAGGAGAATCTTCCAAGAAA+AGG 0.495328 2_3:+59086769 None:intergenic
ATATTATTAGGGATATGTTA+AGG 0.495652 2_3:-59084089 Msa0302290:three_prime_UTR
TCTTTAAGCAATGGGTTCCA+TGG 0.504864 2_3:-59086891 Msa0302290:CDS
ACCCACAACACATATTGATA+AGG 0.507027 2_3:+59084966 None:intergenic
TACCTCAAAATGGAGTTGTC+AGG 0.507679 2_3:-59084687 Msa0302290:CDS
GGGATGGTTCTACTCTTTCG+AGG 0.511587 2_3:-59084761 Msa0302290:CDS
ATTTCAGGCTCTTTAAGCAA+TGG 0.516293 2_3:-59086900 Msa0302290:CDS
CATTGTTTGGCTTACTCGGA+GGG 0.524954 2_3:-59085367 Msa0302290:CDS
ACGTTGGCAAACACAAAAGT+TGG 0.526318 2_3:+59086862 None:intergenic
CTTTGTTCGCAACTTTGCCA+TGG 0.528366 2_3:-59084409 Msa0302290:CDS
TTCAAAATCATCTATAATCA+CGG 0.533216 2_3:-59087124 Msa0302290:five_prime_UTR
CTTTCCTATTACTCATTGCA+GGG 0.535119 2_3:-59084938 Msa0302290:intron
GTACCATGAAATGCATTCTC+AGG 0.536653 2_3:+59086802 None:intergenic
TCAACATGTTGGCAAGTACA+TGG 0.537008 2_3:+59085737 None:intergenic
AAATCACGATCACGAGTGAC+GGG 0.541550 2_3:+59086919 None:intergenic
TGACAGATTCATTACTGGGA+TGG 0.543513 2_3:-59084777 Msa0302290:intron
TAAACTGAGGACGAATCAAA+AGG 0.544513 2_3:+59085057 None:intergenic
TGCTCTAGAAGTAGATAAAG+TGG 0.545530 2_3:-59085818 Msa0302290:CDS
AGAAATTCGTTGTTCTCAGT+CGG 0.545619 2_3:-59084306 Msa0302290:three_prime_UTR
CACATGCAAAATTTCTTGAA+GGG 0.545781 2_3:-59084375 Msa0302290:three_prime_UTR
AAAACTCACGTAAAGGAAGA+AGG 0.547703 2_3:+59086715 None:intergenic
GACAATTTCGCTCATCTAGG+AGG 0.549429 2_3:-59085110 Msa0302290:CDS
TCTGCGTTATTCATACAAAC+GGG 0.553724 2_3:-59085413 Msa0302290:CDS
AAGAGAGCAAAGGAATGGAA+TGG 0.554489 2_3:-59084168 Msa0302290:three_prime_UTR
ACAAAAGTTGGAACAAGCCA+TGG 0.556908 2_3:+59086874 None:intergenic
ATACAAACGGGCATCTCAGT+TGG 0.569101 2_3:-59085401 Msa0302290:CDS
GAAATCACGATCACGAGTGA+CGG 0.572889 2_3:+59086918 None:intergenic
ACGACGAAGACGGAGACCCA+CGG 0.573842 2_3:+59086953 None:intergenic
ATCAAAAGAGAGCAAAGGAA+TGG 0.574326 2_3:-59084173 Msa0302290:three_prime_UTR
TCAACGGTGGTGCCACCATG+TGG 0.575383 2_3:+59086994 None:intergenic
CATGTGTATCTATCTATCCA+TGG 0.577916 2_3:+59084392 None:intergenic
AAATCATAATCATGAGTGGA+GGG 0.582372 2_3:-59087023 Msa0302290:exon
TTGTATATGGTTGCATGGTG+GGG 0.585338 2_3:-59085764 Msa0302290:CDS
AGGAAGAAGGACCAAAGAGA+GGG 0.589526 2_3:+59086728 None:intergenic
AAACAATTCTCCACAGACAA+AGG 0.590975 2_3:-59087177 Msa0302290:five_prime_UTR
ACAAATTCCTAACTTTGTCA+GGG 0.594817 2_3:-59087227 Msa0302290:five_prime_UTR
TGGTTCATGAACACGAAGGT+TGG 0.597343 2_3:-59085798 Msa0302290:CDS
AACAATGAAAATAATAACGT+TGG 0.597447 2_3:+59086846 None:intergenic
GGAACAAAGCATTTATTGTG+AGG 0.597670 2_3:-59084666 Msa0302290:intron
TTAAGGTTCATCCTCGCCGT+GGG 0.597978 2_3:-59086969 Msa0302290:CDS
TCTTGTATATGGTTGCATGG+TGG 0.604832 2_3:-59085766 Msa0302290:CDS
GCATTGTTTGGCTTACTCGG+AGG 0.606501 2_3:-59085368 Msa0302290:CDS
GTTAAGGTTCATCCTCGCCG+TGG 0.606777 2_3:-59086970 Msa0302290:CDS
CGAAATTGTCAACATGTGGA+AGG 0.611940 2_3:+59085123 None:intergenic
TGAGCGAAATTGTCAACATG+TGG 0.615330 2_3:+59085119 None:intergenic
GGATTCGACGACGACGAAGA+CGG 0.615599 2_3:+59086943 None:intergenic
CAACGGTGGTGCCACCATGT+GGG 0.618108 2_3:+59086995 None:intergenic
AACATCACATGCATGAGCAA+TGG 0.619993 2_3:-59084484 Msa0302290:CDS
ACATGGTGGCACCACCGTTG+AGG 0.621441 2_3:-59086992 Msa0302290:CDS
AAGGAAGAAGGACCAAAGAG+AGG 0.633246 2_3:+59086727 None:intergenic
TGGCTTATCCATTTAAACTG+AGG 0.633333 2_3:+59085044 None:intergenic
AAAATCATAATCATGAGTGG+AGG 0.633390 2_3:-59087024 Msa0302290:exon
CTATTATCAAAAGAGAGCAA+AGG 0.634615 2_3:-59084178 Msa0302290:three_prime_UTR
TCACGATCACGAGTGACGGG+AGG 0.634822 2_3:+59086922 None:intergenic
ACTTGAATGAACGTTGCTCG+TGG 0.663668 2_3:-59084733 Msa0302290:CDS
AGGATGAACCTTAACCTCAA+CGG 0.671763 2_3:+59086978 None:intergenic
TCATAATCATGAGTGGAGGG+AGG 0.674267 2_3:-59087020 Msa0302290:exon
GAAGACGGAGACCCACGGCG+AGG 0.680052 2_3:+59086958 None:intergenic
GAAGAGCTAGTATTCGACAG+AGG 0.682346 2_3:+59084445 None:intergenic
AGTGGAGGGAGGAACCCACA+TGG 0.690830 2_3:-59087009 Msa0302290:CDS
GCAACCATATACAAGAGACA+AGG 0.709780 2_3:+59085773 None:intergenic
AAAATCATCTATAATCACGG+AGG 0.711965 2_3:-59087121 Msa0302290:five_prime_UTR
ATGAACCTTAACCTCAACGG+TGG 0.726735 2_3:+59086981 None:intergenic
GGAGGGAGGAACCCACATGG+TGG 0.736064 2_3:-59087006 Msa0302290:CDS
AAAGTGGTTCATGAACACGA+AGG 0.793505 2_3:-59085802 Msa0302290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAATAAATTATTTATC+AGG + chr2_3:59086100-59086119 None:intergenic 10.0%
!!! TAAATAATTTATTGTTTTTG+CGG - chr2_3:59086102-59086121 Msa0302290:intron 10.0%
!!! ATTATAGATGATTTTGAAAT+GGG + chr2_3:59084231-59084250 None:intergenic 15.0%
!!! CATGATTATGATTTTTTTTT+TGG + chr2_3:59084328-59084347 None:intergenic 15.0%
!! AATGATATAAATACAGATAA+TGG + chr2_3:59085759-59085778 None:intergenic 15.0%
!! ATATTACCTTTAATATTCTT+AGG - chr2_3:59086464-59086483 Msa0302290:intron 15.0%
!!! GTTTTTTCTTTTATCTAAAT+TGG - chr2_3:59086803-59086822 Msa0302290:CDS 15.0%
!! TAATAGTAATAAACTTATGA+TGG + chr2_3:59087102-59087121 None:intergenic 15.0%
!!! GATTATAGATGATTTTGAAA+TGG + chr2_3:59084232-59084251 None:intergenic 20.0%
!! TTCAAAATCATCTATAATCA+CGG - chr2_3:59084233-59084252 Msa0302290:three_prime_UTR 20.0%
!! AAAAAAATCATAATCATGAG+TGG - chr2_3:59084330-59084349 Msa0302290:three_prime_UTR 20.0%
!! AACAATGAAAATAATAACGT+TGG + chr2_3:59084514-59084533 None:intergenic 20.0%
!! TACATTAATCAAGTTATGAT+TGG - chr2_3:59084747-59084766 Msa0302290:CDS 20.0%
!! ATGAACATGTGTTTATAAAT+GGG - chr2_3:59085090-59085109 Msa0302290:CDS 20.0%
!!! TTGTTTTTACATAAATGTTC+TGG - chr2_3:59085777-59085796 Msa0302290:CDS 20.0%
!!! AGTAGTATTTAATCTAAAGT+TGG - chr2_3:59086550-59086569 Msa0302290:intron 20.0%
!!! AATTTTTTTTCCTTTGTCTG+TGG + chr2_3:59084193-59084212 None:intergenic 25.0%
!! ATCTGAAATCTTTGTTCAAA+AGG - chr2_3:59084299-59084318 Msa0302290:three_prime_UTR 25.0%
!! TCTGAAATCTTTGTTCAAAA+GGG - chr2_3:59084300-59084319 Msa0302290:three_prime_UTR 25.0%
! TTAATCAAGTTATGATTGGT+TGG - chr2_3:59084751-59084770 Msa0302290:CDS 25.0%
! CATGAACATGTGTTTATAAA+TGG - chr2_3:59085089-59085108 Msa0302290:CDS 25.0%
! TGAACATGTGTTTATAAATG+GGG - chr2_3:59085091-59085110 Msa0302290:CDS 25.0%
!!! TTAAATTGATTCGAGCTAAA+GGG - chr2_3:59085124-59085143 Msa0302290:CDS 25.0%
! TTCATAGATTACATTCTCTA+TGG - chr2_3:59085331-59085350 Msa0302290:CDS 25.0%
! GCTATTGCAAATACATAATT+TGG + chr2_3:59085410-59085429 None:intergenic 25.0%
! TATGTATTTGCAATAGCTAA+TGG - chr2_3:59085413-59085432 Msa0302290:CDS 25.0%
!! ACAGTACCAATCTTAATTTT+AGG - chr2_3:59085436-59085455 Msa0302290:CDS 25.0%
! TATCTGCCTAAAATTAAGAT+TGG + chr2_3:59085445-59085464 None:intergenic 25.0%
!! ATGTTGAGTCTTCTTTTTAT+TGG - chr2_3:59085636-59085655 Msa0302290:intron 25.0%
!! GTCTTCTTTTTATTGGAATT+CGG - chr2_3:59085643-59085662 Msa0302290:intron 25.0%
!!! TTTTAGTTCATTCACCTTAT+TGG + chr2_3:59086053-59086072 None:intergenic 25.0%
! CAAAATGGAAAGAAATAGAA+TGG + chr2_3:59086140-59086159 None:intergenic 25.0%
! GTTGTAATAAATTTAGCTGT+TGG - chr2_3:59086208-59086227 Msa0302290:intron 25.0%
!!! AATTTTGACAGATTCATTAC+TGG - chr2_3:59086575-59086594 Msa0302290:intron 25.0%
!!! ATTTTGACAGATTCATTACT+GGG - chr2_3:59086576-59086595 Msa0302290:intron 25.0%
! GAACTAGAAAATAGAATTGA+TGG + chr2_3:59086742-59086761 None:intergenic 25.0%
! TTGTGTATATGATTCATTCT+TGG - chr2_3:59087020-59087039 Msa0302290:exon 25.0%
! AAATGCTGTCGAATATTATT+AGG - chr2_3:59087256-59087275 Msa0302290:five_prime_UTR 25.0%
! AATGCTGTCGAATATTATTA+GGG - chr2_3:59087257-59087276 Msa0302290:five_prime_UTR 25.0%
AACAAATTCCTAACTTTGTC+AGG - chr2_3:59084129-59084148 Msa0302290:three_prime_UTR 30.0%
ACAAATTCCTAACTTTGTCA+GGG - chr2_3:59084130-59084149 Msa0302290:three_prime_UTR 30.0%
AAAATCATCTATAATCACGG+AGG - chr2_3:59084236-59084255 Msa0302290:three_prime_UTR 30.0%
AAAATCATAATCATGAGTGG+AGG - chr2_3:59084333-59084352 Msa0302290:three_prime_UTR 30.0%
AAATCATAATCATGAGTGGA+GGG - chr2_3:59084334-59084353 Msa0302290:three_prime_UTR 30.0%
! GTTTTCTTTAAGTGGTTGAA+AGG + chr2_3:59084610-59084629 None:intergenic 30.0%
GAAAAAGAAAACTCACGTAA+AGG + chr2_3:59084652-59084671 None:intergenic 30.0%
TGAGAATGAGTTTATTAGTG+TGG - chr2_3:59084872-59084891 Msa0302290:intron 30.0%
! ACTTTAGATGTCTAAGATTG+AGG - chr2_3:59085027-59085046 Msa0302290:CDS 30.0%
! CTTTAGATGTCTAAGATTGA+GGG - chr2_3:59085028-59085047 Msa0302290:CDS 30.0%
GAACATGTGTTTATAAATGG+GGG - chr2_3:59085092-59085111 Msa0302290:CDS 30.0%
AACATGTGTTTATAAATGGG+GGG - chr2_3:59085093-59085112 Msa0302290:CDS 30.0%
!! GTTAAATTGATTCGAGCTAA+AGG - chr2_3:59085123-59085142 Msa0302290:CDS 30.0%
TTAATATTTACTCCTTCGTC+CGG + chr2_3:59085179-59085198 None:intergenic 30.0%
AAAAACCTAATAGATGTGTG+AGG - chr2_3:59085272-59085291 Msa0302290:intron 30.0%
AAAACCTAATAGATGTGTGA+GGG - chr2_3:59085273-59085292 Msa0302290:intron 30.0%
GAATGTAATCTATGAATCAC+CGG + chr2_3:59085327-59085346 None:intergenic 30.0%
! TTTTAGGCAGATATATCTCT+TGG - chr2_3:59085452-59085471 Msa0302290:CDS 30.0%
! ATTTTCAGATTACAGGAGAT+GGG - chr2_3:59085516-59085535 Msa0302290:intron 30.0%
AAAAAGAAGACTCAACATGT+TGG + chr2_3:59085634-59085653 None:intergenic 30.0%
CTTGAACAAGAATTTGGATT+TGG - chr2_3:59085666-59085685 Msa0302290:intron 30.0%
! ACAAGAATTTGGATTTGGTA+TGG - chr2_3:59085671-59085690 Msa0302290:intron 30.0%
!! TACAGATAATGGAATACTGT+TGG + chr2_3:59085748-59085767 None:intergenic 30.0%
GGAAAGAAATAGAATGGTTT+AGG + chr2_3:59086134-59086153 None:intergenic 30.0%
CAAAACATCATGAGACAAAA+TGG + chr2_3:59086155-59086174 None:intergenic 30.0%
TACCTTATCAATATGTGTTG+TGG - chr2_3:59086389-59086408 Msa0302290:intron 30.0%
ACCTTATCAATATGTGTTGT+GGG - chr2_3:59086390-59086409 Msa0302290:intron 30.0%
CCAAGTCCTAAGAATATTAA+AGG + chr2_3:59086473-59086492 None:intergenic 30.0%
CCTTTAATATTCTTAGGACT+TGG - chr2_3:59086470-59086489 Msa0302290:intron 30.0%
ACAATATTCTATGCAGACAA+CGG - chr2_3:59086833-59086852 Msa0302290:CDS 30.0%
ACACATGCAAAATTTCTTGA+AGG - chr2_3:59086981-59087000 Msa0302290:CDS 30.0%
CACATGCAAAATTTCTTGAA+GGG - chr2_3:59086982-59087001 Msa0302290:CDS 30.0%
AGTAATAAACTTATGATGGC+TGG + chr2_3:59087098-59087117 None:intergenic 30.0%
CTATTATCAAAAGAGAGCAA+AGG - chr2_3:59087179-59087198 Msa0302290:five_prime_UTR 30.0%
AATGGAATGGAAGATGTATT+TGG - chr2_3:59087202-59087221 Msa0302290:five_prime_UTR 30.0%
AAACAATTCTCCACAGACAA+AGG - chr2_3:59084180-59084199 Msa0302290:three_prime_UTR 35.0%
! GAAATGGGTTTGAATCAGTT+AGG + chr2_3:59084216-59084235 None:intergenic 35.0%
ATTTCAGGCTCTTTAAGCAA+TGG - chr2_3:59084457-59084476 Msa0302290:CDS 35.0%
TTTCAGGCTCTTTAAGCAAT+GGG - chr2_3:59084458-59084477 Msa0302290:CDS 35.0%
AAGGAGAATCTTCCAAGAAA+AGG + chr2_3:59084591-59084610 None:intergenic 35.0%
! AAGAGAGGGTTTTCTTTAAG+TGG + chr2_3:59084618-59084637 None:intergenic 35.0%
CTTAAAGAAAACCCTCTCTT+TGG - chr2_3:59084618-59084637 Msa0302290:intron 35.0%
AAAACTCACGTAAAGGAAGA+AGG + chr2_3:59084645-59084664 None:intergenic 35.0%
! ATGTCTAAGATTGAGGGTAA+GGG - chr2_3:59085034-59085053 Msa0302290:CDS 35.0%
TAATATTTACTCCTTCGTCC+GGG + chr2_3:59085178-59085197 None:intergenic 35.0%
GAAACAAACGTGTGTTTGTA+AGG - chr2_3:59085231-59085250 Msa0302290:intron 35.0%
! TACTCGCATTTTCAGATTAC+AGG - chr2_3:59085509-59085528 Msa0302290:intron 35.0%
! CATTTTCAGATTACAGGAGA+TGG - chr2_3:59085515-59085534 Msa0302290:intron 35.0%
!! TGCTCTAGAAGTAGATAAAG+TGG - chr2_3:59085539-59085558 Msa0302290:intron 35.0%
! ATTCGGCTTGAACAAGAATT+TGG - chr2_3:59085660-59085679 Msa0302290:intron 35.0%
! ATTTGGATTTGGTATGGTTC+TGG - chr2_3:59085677-59085696 Msa0302290:intron 35.0%
CGATATACTTGAACAATGCA+AGG + chr2_3:59085710-59085729 None:intergenic 35.0%
AATCCAATTCTAACTGCGAA+AGG + chr2_3:59085887-59085906 None:intergenic 35.0%
GGATTTCTCTATGTGATATC+TGG - chr2_3:59085902-59085921 Msa0302290:intron 35.0%
!! CTCTATGTGATATCTGGTTT+TGG - chr2_3:59085908-59085927 Msa0302290:intron 35.0%
!!! TATGTGATATCTGGTTTTGG+AGG - chr2_3:59085911-59085930 Msa0302290:intron 35.0%
!!! ATGTGATATCTGGTTTTGGA+GGG - chr2_3:59085912-59085931 Msa0302290:intron 35.0%
GTCTGCGTTATTCATACAAA+CGG - chr2_3:59085943-59085962 Msa0302290:intron 35.0%
TCTGCGTTATTCATACAAAC+GGG - chr2_3:59085944-59085963 Msa0302290:intron 35.0%
TCTCAGAGCTCTTCATTAAT+TGG - chr2_3:59086017-59086036 Msa0302290:intron 35.0%
TTGGACGATATATGCCAATA+AGG - chr2_3:59086036-59086055 Msa0302290:intron 35.0%
TAAACTGAGGACGAATCAAA+AGG + chr2_3:59086303-59086322 None:intergenic 35.0%
TGATTCGTCCTCAGTTTAAA+TGG - chr2_3:59086305-59086324 Msa0302290:intron 35.0%
TGGCTTATCCATTTAAACTG+AGG + chr2_3:59086316-59086335 None:intergenic 35.0%
ATCCAGATTTAGACTGTTGT+TGG + chr2_3:59086336-59086355 None:intergenic 35.0%
ACCCACAACACATATTGATA+AGG + chr2_3:59086394-59086413 None:intergenic 35.0%
TCTTTCCTATTACTCATTGC+AGG - chr2_3:59086418-59086437 Msa0302290:intron 35.0%
CTTTCCTATTACTCATTGCA+GGG - chr2_3:59086419-59086438 Msa0302290:intron 35.0%
! GGAACAAAGCATTTATTGTG+AGG - chr2_3:59086691-59086710 Msa0302290:intron 35.0%
CATGTGTATCTATCTATCCA+TGG + chr2_3:59086968-59086987 None:intergenic 35.0%
AGAAATTCGTTGTTCTCAGT+CGG - chr2_3:59087051-59087070 Msa0302290:five_prime_UTR 35.0%
ATCAAAAGAGAGCAAAGGAA+TGG - chr2_3:59087184-59087203 Msa0302290:five_prime_UTR 35.0%
AACAAGACCCTGACAAAGTT+AGG + chr2_3:59084140-59084159 None:intergenic 40.0%
! TAACTTTGTCAGGGTCTTGT+TGG - chr2_3:59084139-59084158 Msa0302290:three_prime_UTR 40.0%
AGGATGAACCTTAACCTCAA+CGG + chr2_3:59084382-59084401 None:intergenic 40.0%
! TCTTTAAGCAATGGGTTCCA+TGG - chr2_3:59084466-59084485 Msa0302290:CDS 40.0%
ACAAAAGTTGGAACAAGCCA+TGG + chr2_3:59084486-59084505 None:intergenic 40.0%
ACGTTGGCAAACACAAAAGT+TGG + chr2_3:59084498-59084517 None:intergenic 40.0%
TGTCCTGAGAATGCATTTCA+TGG - chr2_3:59084552-59084571 Msa0302290:intron 40.0%
GTACCATGAAATGCATTCTC+AGG + chr2_3:59084558-59084577 None:intergenic 40.0%
!! ACTTGTGTTGCTCCTTTTCT+TGG - chr2_3:59084576-59084595 Msa0302290:intron 40.0%
!! GCCAGTTTTGTGAATGAGTT+AGG - chr2_3:59084913-59084932 Msa0302290:intron 40.0%
GCCTAACTCATTCACAAAAC+TGG + chr2_3:59084917-59084936 None:intergenic 40.0%
! GATGTCTAAGATTGAGGGTA+AGG - chr2_3:59085033-59085052 Msa0302290:CDS 40.0%
TGTGTTTATAAATGGGGGGT+TGG - chr2_3:59085097-59085116 Msa0302290:CDS 40.0%
GTGTTTATAAATGGGGGGTT+GGG - chr2_3:59085098-59085117 Msa0302290:CDS 40.0%
GAGCTAAAGGGATAAGAAGT+TGG - chr2_3:59085136-59085155 Msa0302290:CDS 40.0%
CTAAAGGGATAAGAAGTTGG+AGG - chr2_3:59085139-59085158 Msa0302290:CDS 40.0%
GTGTGTTTGTAAGGATGCAT+AGG - chr2_3:59085240-59085259 Msa0302290:intron 40.0%
ACAACCCTCACACATCTATT+AGG + chr2_3:59085280-59085299 None:intergenic 40.0%
AAAGTGGTTCATGAACACGA+AGG - chr2_3:59085555-59085574 Msa0302290:intron 40.0%
GCAACCATATACAAGAGACA+AGG + chr2_3:59085587-59085606 None:intergenic 40.0%
GTCTCTTGTATATGGTTGCA+TGG - chr2_3:59085588-59085607 Msa0302290:intron 40.0%
TCTTGTATATGGTTGCATGG+TGG - chr2_3:59085591-59085610 Msa0302290:intron 40.0%
CTTGTATATGGTTGCATGGT+GGG - chr2_3:59085592-59085611 Msa0302290:intron 40.0%
TTGTATATGGTTGCATGGTG+GGG - chr2_3:59085593-59085612 Msa0302290:intron 40.0%
TCAACATGTTGGCAAGTACA+TGG + chr2_3:59085623-59085642 None:intergenic 40.0%
TAACTGCGAAAGGACAAGAA+AGG + chr2_3:59085877-59085896 None:intergenic 40.0%
TGTCCTTTCGCAGTTAGAAT+TGG - chr2_3:59085881-59085900 Msa0302290:intron 40.0%
! GTCTCATGATGTTTTGCAGA+TGG - chr2_3:59086159-59086178 Msa0302290:intron 40.0%
CGAAATTGTCAACATGTGGA+AGG + chr2_3:59086237-59086256 None:intergenic 40.0%
TGAGCGAAATTGTCAACATG+TGG + chr2_3:59086241-59086260 None:intergenic 40.0%
GTTGACAATTTCGCTCATCT+AGG - chr2_3:59086244-59086263 Msa0302290:intron 40.0%
AGCCAACAACAGTCTAAATC+TGG - chr2_3:59086331-59086350 Msa0302290:intron 40.0%
CTTACCCTGCAATGAGTAAT+AGG + chr2_3:59086426-59086445 None:intergenic 40.0%
TGACAGATTCATTACTGGGA+TGG - chr2_3:59086580-59086599 Msa0302290:intron 40.0%
GTTGTGTTCCTACCTCAAAA+TGG - chr2_3:59086660-59086679 Msa0302290:intron 40.0%
!!! TGACAACTCCATTTTGAGGT+AGG + chr2_3:59086671-59086690 None:intergenic 40.0%
TACCTCAAAATGGAGTTGTC+AGG - chr2_3:59086670-59086689 Msa0302290:intron 40.0%
! TTCCTGACAACTCCATTTTG+AGG + chr2_3:59086675-59086694 None:intergenic 40.0%
TCTATGCAGACAACGGAATT+CGG - chr2_3:59086840-59086859 Msa0302290:CDS 40.0%
AACATCACATGCATGAGCAA+TGG - chr2_3:59086873-59086892 Msa0302290:CDS 40.0%
AAGAGAGCAAAGGAATGGAA+TGG - chr2_3:59087189-59087208 Msa0302290:five_prime_UTR 40.0%
TCATAATCATGAGTGGAGGG+AGG - chr2_3:59084337-59084356 Msa0302290:three_prime_UTR 45.0%
ATGAACCTTAACCTCAACGG+TGG + chr2_3:59084379-59084398 None:intergenic 45.0%
AAATCACGATCACGAGTGAC+GGG + chr2_3:59084441-59084460 None:intergenic 45.0%
GAAATCACGATCACGAGTGA+CGG + chr2_3:59084442-59084461 None:intergenic 45.0%
TCACTCGTGATCGTGATTTC+AGG - chr2_3:59084442-59084461 Msa0302290:CDS 45.0%
AGGAAGAAGGACCAAAGAGA+GGG + chr2_3:59084632-59084651 None:intergenic 45.0%
AAGGAAGAAGGACCAAAGAG+AGG + chr2_3:59084633-59084652 None:intergenic 45.0%
! CATCTAAAGTGTGACTCGTG+TGG + chr2_3:59085017-59085036 None:intergenic 45.0%
!! GGAGGTTCTAAGTTCAAACC+CGG - chr2_3:59085157-59085176 Msa0302290:CDS 45.0%
TCTTGTGTCTGTGTCTCTAC+CGG - chr2_3:59085305-59085324 Msa0302290:intron 45.0%
TGGTTCATGAACACGAAGGT+TGG - chr2_3:59085559-59085578 Msa0302290:intron 45.0%
ATACAAACGGGCATCTCAGT+TGG - chr2_3:59085956-59085975 Msa0302290:intron 45.0%
! GGTGCATTGTTTGGCTTACT+CGG - chr2_3:59085986-59086005 Msa0302290:intron 45.0%
! CATTGTTTGGCTTACTCGGA+GGG - chr2_3:59085990-59086009 Msa0302290:intron 45.0%
GACAATTTCGCTCATCTAGG+AGG - chr2_3:59086247-59086266 Msa0302290:intron 45.0%
TTCGCTCATCTAGGAGGATT+TGG - chr2_3:59086253-59086272 Msa0302290:intron 45.0%
! CATCTAGGAGGATTTGGTTC+TGG - chr2_3:59086259-59086278 Msa0302290:intron 45.0%
!! TTTGGTTCTGGCTTTCTGCT+TGG - chr2_3:59086271-59086290 Msa0302290:intron 45.0%
ACTTGAATGAACGTTGCTCG+TGG - chr2_3:59086624-59086643 Msa0302290:intron 45.0%
GAAGAGCTAGTATTCGACAG+AGG + chr2_3:59086915-59086934 None:intergenic 45.0%
CTTTGTTCGCAACTTTGCCA+TGG - chr2_3:59086948-59086967 Msa0302290:CDS 45.0%
TTGTTCTCAGTCGGCAATGA+TGG - chr2_3:59087060-59087079 Msa0302290:five_prime_UTR 45.0%
!! GTCAGGGTCTTGTTGGATTC+TGG - chr2_3:59084146-59084165 Msa0302290:three_prime_UTR 50.0%
TTAAGGTTCATCCTCGCCGT+GGG - chr2_3:59084388-59084407 Msa0302290:exon 50.0%
! GATAATGGTGGCCAGATAGC+AGG + chr2_3:59084991-59085010 None:intergenic 50.0%
GCTATCTGGCCACCATTATC+AGG - chr2_3:59084991-59085010 Msa0302290:CDS 50.0%
TGACTCGTGTGGCCTGATAA+TGG + chr2_3:59085006-59085025 None:intergenic 50.0%
CTAAGTTCAAACCCGGACGA+AGG - chr2_3:59085164-59085183 Msa0302290:CDS 50.0%
GGCGCCTTGTCTCTTGTATA+TGG - chr2_3:59085580-59085599 Msa0302290:intron 50.0%
GGTGCATCTGGTGCATTGTT+TGG - chr2_3:59085977-59085996 Msa0302290:intron 50.0%
! GCATTGTTTGGCTTACTCGG+AGG - chr2_3:59085989-59086008 Msa0302290:intron 50.0%
! GGGATGGTTCTACTCTTTCG+AGG - chr2_3:59086596-59086615 Msa0302290:intron 50.0%
!! GGGTCTTGTTGGATTCTGGC+TGG - chr2_3:59084150-59084169 Msa0302290:three_prime_UTR 55.0%
TGGCACCACCGTTGAGGTTA+AGG - chr2_3:59084371-59084390 Msa0302290:three_prime_UTR 55.0%
GTTAAGGTTCATCCTCGCCG+TGG - chr2_3:59084387-59084406 Msa0302290:exon 55.0%
GGATTCGACGACGACGAAGA+CGG + chr2_3:59084417-59084436 None:intergenic 55.0%
GCAGGCGGTTCAATGGATCT+TGG + chr2_3:59084973-59084992 None:intergenic 55.0%
CCAGATAGCAGGCGGTTCAA+TGG + chr2_3:59084980-59084999 None:intergenic 55.0%
CCATTGAACCGCCTGCTATC+TGG - chr2_3:59084977-59084996 Msa0302290:CDS 55.0%
AATGGTGGCCAGATAGCAGG+CGG + chr2_3:59084988-59085007 None:intergenic 55.0%
!! CTCGTGTGGCCTGATAATGG+TGG + chr2_3:59085003-59085022 None:intergenic 55.0%
! GGCATCTCAGTTGGTGCATC+TGG - chr2_3:59085965-59085984 Msa0302290:intron 55.0%
AGTGGAGGGAGGAACCCACA+TGG - chr2_3:59084348-59084367 Msa0302290:three_prime_UTR 60.0%
!! CAACGGTGGTGCCACCATGT+GGG + chr2_3:59084365-59084384 None:intergenic 60.0%
!! TCAACGGTGGTGCCACCATG+TGG + chr2_3:59084366-59084385 None:intergenic 60.0%
ACATGGTGGCACCACCGTTG+AGG - chr2_3:59084365-59084384 Msa0302290:three_prime_UTR 60.0%
ACGACGAAGACGGAGACCCA+CGG + chr2_3:59084407-59084426 None:intergenic 60.0%
! TCACGATCACGAGTGACGGG+AGG + chr2_3:59084438-59084457 None:intergenic 60.0%
GGAGGGAGGAACCCACATGG+TGG - chr2_3:59084351-59084370 Msa0302290:three_prime_UTR 65.0%
GAAGACGGAGACCCACGGCG+AGG + chr2_3:59084402-59084421 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 59084089 59087290 59084089 ID=Msa0302290;Name=Msa0302290
chr2_3 mRNA 59084089 59087290 59084089 ID=Msa0302290-mRNA-1;Parent=Msa0302290;Name=Msa0302290-mRNA-1;_AED=0.07;_eAED=0.07;_QI=256|1|1|1|1|1|6|316|384
chr2_3 exon 59084089 59084530 59084089 ID=Msa0302290-mRNA-1:exon:19083;Parent=Msa0302290-mRNA-1
chr2_3 exon 59084667 59084793 59084667 ID=Msa0302290-mRNA-1:exon:19082;Parent=Msa0302290-mRNA-1
chr2_3 exon 59084939 59085201 59084939 ID=Msa0302290-mRNA-1:exon:19081;Parent=Msa0302290-mRNA-1
chr2_3 exon 59085322 59085485 59085322 ID=Msa0302290-mRNA-1:exon:19080;Parent=Msa0302290-mRNA-1
chr2_3 exon 59085692 59085855 59085692 ID=Msa0302290-mRNA-1:exon:19079;Parent=Msa0302290-mRNA-1
chr2_3 exon 59086724 59087290 59086724 ID=Msa0302290-mRNA-1:exon:19078;Parent=Msa0302290-mRNA-1
chr2_3 five_prime_UTR 59087035 59087290 59087035 ID=Msa0302290-mRNA-1:five_prime_utr;Parent=Msa0302290-mRNA-1
chr2_3 CDS 59086724 59087034 59086724 ID=Msa0302290-mRNA-1:cds;Parent=Msa0302290-mRNA-1
chr2_3 CDS 59085692 59085855 59085692 ID=Msa0302290-mRNA-1:cds;Parent=Msa0302290-mRNA-1
chr2_3 CDS 59085322 59085485 59085322 ID=Msa0302290-mRNA-1:cds;Parent=Msa0302290-mRNA-1
chr2_3 CDS 59084939 59085201 59084939 ID=Msa0302290-mRNA-1:cds;Parent=Msa0302290-mRNA-1
chr2_3 CDS 59084667 59084793 59084667 ID=Msa0302290-mRNA-1:cds;Parent=Msa0302290-mRNA-1
chr2_3 CDS 59084405 59084530 59084405 ID=Msa0302290-mRNA-1:cds;Parent=Msa0302290-mRNA-1
chr2_3 three_prime_UTR 59084089 59084404 59084089 ID=Msa0302290-mRNA-1:three_prime_utr;Parent=Msa0302290-mRNA-1
Gene Sequence

>Msa0302290

ATGAGTGGAGGGAGGAACCCACATGGTGGCACCACCGTTGAGGTTAAGGTTCATCCTCGCCGTGGGTCTCCGTCTTCGTCGTCGTCGAATCCTCCCGTCACTCGTGATCGTGATTTCAGGCTCTTTAAGCAATGGGTTCCATGGCTTGTTCCAACTTTTGTGTTTGCCAACGTTATTATTTTCATTGTTACAATGTATGTTAATGATTGTCCTGAGAATGCATTTCATGGTACTTGTGTTGCTCCTTTTCTTGGAAGATTCTCCTTTCAACCACTTAAAGAAAACCCTCTCTTTGGTCCTTCTTCCTTTACATTACAGGAGATGGGTGCTCTAGAAGTAGATAAAGTGGTTCATGAACACGAAGGTTGGCGCCTTGTCTCTTGTATATGGTTGCATGGTGGGGCTATCCATGTACTTGCCAACATGTTGAGTCTTCTTTTTATTGGAATTCGGCTTGAACAAGAATTTGGATTTGTTAGAATTGGATTTCTCTATGTGATATCTGGTTTTGGAGGGAGTTTGCTGTCTGCGTTATTCATACAAACGGGCATCTCAGTTGGTGCATCTGGTGCATTGTTTGGCTTACTCGGAGGGATGCTCTCAGAGCTCTTCATTAATTGGACGATATATGCCAATAAGATGGCTGCATTGTTGACTCTTATAGTCATTGTTGTAATAAATTTAGCTGTTGGAGTCCTTCCACATGTTGACAATTTCGCTCATCTAGGAGGATTTGGTTCTGGCTTTCTGCTTGGATTTGTCCTTTTGATTCGTCCTCAGTTTAAATGGATAAGCCAACAACAGTCTAAATCTGGATCTCATGCATTTTCTGTTAAACACAATAAGTACTTACCTTATCAATATGTGTTGTGGGTTTTGTCTTTCCTATTACTCATTGCAGGATTCATTACTGGGATGGTTCTACTCTTTCGAGGTGTTAACTTGAATGAACGTTGCTCGTGGTGTCATTATTTAAGTTGTGTTCCTACCTCAAAATGGAGTTGTCAGGAACAAAGCATTTATTGTGAGACAACGGAATTCGGAAATCAACTTAACATCACATGCATGAGCAATGGTAGAAGTGACATTTATCCTCTGTCGAATACTAGCTCTTCTGATAGACAACAACTTTGTTCGCAACTTTGCCATGGATAG
Protein sequence

>Msa0302290

MSGGRNPHGGTTVEVKVHPRRGSPSSSSSNPPVTRDRDFRLFKQWVPWLVPTFVFANVIIFIVTMYVNDCPENAFHGTCVAPFLGRFSFQPLKENPLFGPSSFTLQEMGALEVDKVVHEHEGWRLVSCIWLHGGAIHVLANMLSLLFIGIRLEQEFGFVRIGFLYVISGFGGSLLSALFIQTGISVGASGALFGLLGGMLSELFINWTIYANKMAALLTLIVIVVINLAVGVLPHVDNFAHLGGFGSGFLLGFVLLIRPQFKWISQQQSKSGSHAFSVKHNKYLPYQYVLWVLSFLLLIAGFITGMVLLFRGVNLNERCSWCHYLSCVPTSKWSCQEQSIYCETTEFGNQLNITCMSNGRSDIYPLSNTSSSDRQQLCSQLCHG*