AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0369880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0369880 MtrunA17_Chr3g0114701 97.416 387 10 0 1 387 1 387 0.0 777
Msa0369880 MtrunA17_Chr1g0160181 67.018 379 120 1 1 379 10 383 0.0 513
Msa0369880 MtrunA17_Chr3g0114731 50.526 380 173 5 7 386 16 380 8.13e-137 396
Msa0369880 MtrunA17_Chr3g0114711 48.363 397 152 6 5 360 10 394 1.87e-125 367
Msa0369880 MtrunA17_Chr1g0177221 64.844 256 87 1 3 258 12 264 1.99e-118 344
Msa0369880 MtrunA17_Chr1g0160171 45.283 371 189 4 1 371 8 364 5.01e-114 337
Msa0369880 MtrunA17_Chr7g0255561 45.783 332 177 2 3 332 8 338 8.44e-110 327
Msa0369880 MtrunA17_Chr7g0255581 45.783 332 177 2 3 332 8 338 8.44e-110 327
Msa0369880 MtrunA17_Chr3g0114691 41.417 367 205 2 2 368 13 369 7.56e-109 325
Msa0369880 MtrunA17_Chr1g0182291 43.626 353 189 3 4 356 10 352 2.06e-108 322
Msa0369880 MtrunA17_Chr7g0263101 41.885 382 209 3 6 387 10 378 2.68e-106 318
Msa0369880 MtrunA17_Chr8g0354121 42.188 384 192 6 5 378 9 372 3.58e-102 308
Msa0369880 MtrunA17_Chr3g0081421 45.152 330 177 4 4 330 12 340 1.53e-100 303
Msa0369880 MtrunA17_Chr3g0081511 41.388 389 209 7 2 387 10 382 1.30e-98 298
Msa0369880 MtrunA17_Chr1g0186641 39.571 326 193 2 5 330 9 330 1.61e-79 248
Msa0369880 MtrunA17_Chr1g0186601 38.957 326 195 2 5 330 12 333 1.78e-79 248
Msa0369880 MtrunA17_Chr1g0186571 37.176 347 210 3 5 351 9 347 6.96e-78 244
Msa0369880 MtrunA17_Chr1g0186611 36.415 357 215 4 6 362 13 357 2.03e-77 243
Msa0369880 MtrunA17_Chr1g0186561 38.769 325 196 1 6 330 13 334 1.05e-76 241
Msa0369880 MtrunA17_Chr1g0171301 35.613 351 220 2 6 356 13 357 3.31e-76 239
Msa0369880 MtrunA17_Chr1g0155761 35.279 377 226 3 11 370 23 398 1.08e-71 229
Msa0369880 MtrunA17_Chr3g0142131 35.593 354 215 4 14 355 25 377 3.14e-71 228
Msa0369880 MtrunA17_Chr1g0184221 34.959 369 209 7 9 359 16 371 4.18e-69 222
Msa0369880 MtrunA17_Chr3g0129601 35.380 342 215 4 5 342 25 364 1.30e-68 220
Msa0369880 MtrunA17_Chr6g0463971 35.393 356 209 6 5 355 12 351 3.67e-67 216
Msa0369880 MtrunA17_Chr3g0129571 34.637 358 227 5 10 362 8 363 6.17e-66 213
Msa0369880 MtrunA17_Chr6g0483401 37.278 338 200 5 18 348 19 351 8.36e-66 213
Msa0369880 MtrunA17_Chr4g0050881 35.440 364 222 6 1 361 1 354 1.76e-63 207
Msa0369880 MtrunA17_Chr2g0278561 34.946 372 236 3 9 379 15 381 3.39e-63 207
Msa0369880 MtrunA17_Chr2g0284261 33.983 359 229 4 9 366 8 359 1.63e-61 202
Msa0369880 MtrunA17_Chr8g0383731 33.143 350 225 4 5 348 1 347 4.38e-61 200
Msa0369880 MtrunA17_Chr1g0164831 33.235 340 221 4 9 347 3 337 5.50e-61 200
Msa0369880 MtrunA17_Chr7g0271561 32.822 326 213 3 10 330 8 332 2.10e-60 199
Msa0369880 MtrunA17_Chr2g0278541 32.741 394 241 9 2 374 3 393 3.61e-60 199
Msa0369880 MtrunA17_Chr3g0129591 33.146 356 231 4 10 361 6 358 5.89e-60 198
Msa0369880 MtrunA17_Chr2g0278551 32.361 377 241 3 9 380 12 379 1.28e-59 198
Msa0369880 MtrunA17_Chr2g0290611 32.222 360 243 1 5 364 8 366 5.08e-58 193
Msa0369880 MtrunA17_Chr8g0334761 32.869 359 236 4 10 366 11 366 2.88e-57 191
Msa0369880 MtrunA17_Chr1g0172881 54.491 167 58 2 92 258 1 149 6.38e-55 178
Msa0369880 MtrunA17_Chr2g0332001 31.692 325 217 2 9 332 38 358 3.03e-54 184
Msa0369880 MtrunA17_Chr4g0051021 30.645 372 250 5 5 372 10 377 3.62e-54 183
Msa0369880 MtrunA17_Chr3g0126101 30.854 363 231 6 6 368 3 345 9.15e-53 179
Msa0369880 MtrunA17_Chr2g0310441 34.259 324 205 5 10 330 11 329 2.42e-52 178
Msa0369880 MtrunA17_Chr4g0059311 31.395 344 230 5 4 344 6 346 2.67e-52 177
Msa0369880 MtrunA17_Chr3g0129581 32.773 357 231 5 10 361 6 358 3.61e-52 177
Msa0369880 MtrunA17_Chr4g0050891 32.716 324 214 3 5 325 9 331 1.28e-51 176
Msa0369880 MtrunA17_Chr4g0050941 34.218 339 216 4 5 337 8 345 3.77e-51 176
Msa0369880 MtrunA17_Chr5g0413951 31.447 318 213 4 14 330 12 325 8.20e-50 171
Msa0369880 MtrunA17_Chr5g0413941 30.247 324 221 4 14 336 12 331 1.85e-49 170
Msa0369880 MtrunA17_Chr5g0413961 32.335 334 209 7 10 338 15 336 1.50e-48 168
Msa0369880 MtrunA17_Chr1g0160111 27.635 351 239 6 18 356 16 363 1.80e-48 168
Msa0369880 MtrunA17_Chr8g0334751 33.718 347 218 4 10 351 12 351 7.27e-48 166
Msa0369880 MtrunA17_Chr8g0334771 33.540 322 210 3 10 330 11 329 1.17e-47 166
Msa0369880 MtrunA17_Chr5g0408001 28.756 386 248 8 3 367 8 387 6.33e-47 164
Msa0369880 MtrunA17_Chr5g0407971 29.558 362 237 6 9 356 11 368 1.34e-46 163
Msa0369880 MtrunA17_Chr5g0408031 30.548 347 216 6 6 333 7 347 5.42e-46 162
Msa0369880 MtrunA17_Chr4g0073241 27.577 359 249 6 10 364 9 360 9.36e-45 158
Msa0369880 MtrunA17_Chr5g0408041 28.729 362 241 4 9 355 11 370 2.68e-44 157
Msa0369880 MtrunA17_Chr5g0413931 30.814 344 226 9 13 351 10 346 4.62e-44 156
Msa0369880 MtrunA17_Chr5g0413921 30.503 318 216 4 14 330 12 325 7.63e-44 155
Msa0369880 MtrunA17_Chr7g0227921 31.229 301 201 4 31 330 33 328 8.72e-43 153
Msa0369880 MtrunA17_Chr1g0150151 27.246 345 246 4 6 348 8 349 1.08e-42 152
Msa0369880 MtrunA17_Chr5g0428881 27.200 375 268 4 1 372 10 382 1.23e-42 153
Msa0369880 MtrunA17_Chr5g0407991 28.460 383 244 8 9 368 9 384 1.50e-42 153
Msa0369880 MtrunA17_Chr8g0369841 27.119 354 242 5 17 357 17 367 5.55e-41 148
Msa0369880 MtrunA17_Chr4g0050931 28.080 349 206 5 5 348 8 316 4.18e-40 144
Msa0369880 MtrunA17_Chr5g0404811 30.417 240 164 2 122 361 6 242 3.15e-36 132
Msa0369880 MtrunA17_Chr3g0126091 26.879 346 242 6 10 352 12 349 5.24e-36 134
Msa0369880 MtrunA17_Chr6g0451201 30.060 336 226 6 31 366 34 360 6.23e-36 134
Msa0369880 MtrunA17_Chr6g0451171 27.778 360 247 7 10 366 13 362 2.84e-35 132
Msa0369880 MtrunA17_Chr4g0051001 28.296 311 209 3 5 308 27 330 4.11e-35 131
Msa0369880 MtrunA17_Chr1g0172891 60.360 111 33 2 265 375 13 112 8.50e-35 124
Msa0369880 MtrunA17_Chr1g0177231 57.391 115 35 3 251 362 18 121 9.81e-34 122
Msa0369880 MtrunA17_Chr2g0278021 34.862 218 124 6 164 372 54 262 1.13e-33 126
Msa0369880 MtrunA17_Chr5g0441761 26.567 335 214 5 23 357 40 342 5.64e-33 126
Msa0369880 MtrunA17_Chr1g0182261 43.165 139 77 2 198 334 76 214 1.59e-31 119
Msa0369880 MtrunA17_Chr6g0451251 30.091 329 219 6 31 355 34 355 1.35e-30 120
Msa0369880 MtrunA17_Chr6g0451211 26.667 330 230 8 31 355 34 356 3.21e-30 119
Msa0369880 MtrunA17_Chr3g0084091 26.936 297 203 6 94 387 15 300 6.79e-30 117
Msa0369880 MtrunA17_Chr1g0211801 30.233 258 160 5 9 258 11 256 1.11e-29 115
Msa0369880 MtrunA17_Chr5g0429001 25.072 347 216 6 61 387 3 325 2.09e-29 116
Msa0369880 MtrunA17_Chr6g0451191 29.675 246 164 5 123 368 1 237 5.97e-29 112
Msa0369880 MtrunA17_Chr6g0451261 26.059 307 222 4 6 311 8 310 1.14e-28 118
Msa0369880 MtrunA17_Chr4g0056081 28.000 350 226 9 14 353 19 352 1.80e-27 111
Msa0369880 MtrunA17_Chr5g0399281 28.529 333 213 8 14 334 13 332 4.71e-25 104
Msa0369880 MtrunA17_Chr8g0334711 34.184 196 125 3 10 204 11 203 7.00e-23 95.5
Msa0369880 MtrunA17_Chr8g0334721 40.476 126 74 1 221 346 1 125 3.80e-22 92.0
Msa0369880 MtrunA17_Chr5g0404801 41.026 117 69 0 6 122 4 120 4.89e-20 85.1
Msa0369880 MtrunA17_Chr2g0278031 36.752 117 74 0 5 121 10 126 4.52e-13 65.9
Msa0369880 MtrunA17_Chr4g0050921 33.010 103 68 1 209 311 3 104 7.50e-13 65.1
Msa0369880 MtrunA17_Chr4g0021721 53.333 45 21 0 238 282 2 46 2.18e-12 61.6
Msa0369880 MtrunA17_Chr8g0372571 53.333 45 21 0 238 282 2 46 2.24e-12 62.0
Msa0369880 MtrunA17_Chr1g0211811 36.782 87 55 0 260 346 1 87 5.61e-12 61.6
Msa0369880 MtrunA17_Chr6g0451241 36.905 84 50 2 274 355 23 105 9.47e-12 61.6
Msa0369880 MtrunA17_Chr4g0050911 27.966 118 78 2 94 204 16 133 9.71e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0369880 AT4G08290.1 47.541 366 182 4 3 368 6 361 2.06e-122 359
Msa0369880 AT2G39510.1 43.005 386 198 5 5 383 3 373 1.83e-106 318
Msa0369880 AT2G37460.1 40.374 374 209 2 5 378 10 369 6.79e-105 314
Msa0369880 AT1G21890.1 42.239 393 203 6 1 383 1 379 1.32e-103 311
Msa0369880 AT1G44800.1 40.860 372 205 5 2 371 3 361 8.98e-101 303
Msa0369880 AT4G08300.1 40.162 371 216 4 2 369 3 370 8.56e-96 291
Msa0369880 AT5G07050.1 41.317 334 189 3 3 330 11 343 1.65e-94 288
Msa0369880 AT2G40900.1 40.816 343 187 4 6 348 7 333 3.18e-94 288
Msa0369880 AT1G21890.2 45.570 316 163 3 1 308 1 315 6.51e-91 278
Msa0369880 AT4G08290.2 48.996 249 126 1 9 257 12 259 3.72e-86 263
Msa0369880 AT3G56620.1 40.867 323 182 3 6 327 7 321 3.01e-85 264
Msa0369880 AT2G37450.2 41.061 358 175 4 3 355 2 328 3.40e-84 260
Msa0369880 AT5G13670.1 36.885 366 212 5 6 364 4 357 1.34e-79 249
Msa0369880 AT1G75500.1 37.690 329 195 5 11 332 21 346 3.98e-71 228
Msa0369880 AT1G75500.2 37.690 329 195 5 11 332 21 346 3.98e-71 228
Msa0369880 AT3G18200.2 35.608 337 206 5 14 346 39 368 3.82e-66 215
Msa0369880 AT2G37450.3 43.609 266 135 3 95 355 1 256 4.36e-66 211
Msa0369880 AT3G53210.1 34.711 363 227 6 6 361 6 365 4.28e-64 209
Msa0369880 AT5G64700.1 34.211 342 217 5 5 339 3 343 4.12e-62 204
Msa0369880 AT2G37450.1 44.737 228 116 2 122 349 61 278 3.76e-56 186
Msa0369880 AT2G37450.1 46.667 60 32 0 3 62 2 61 5.22e-11 63.5
Msa0369880 AT4G30420.2 31.152 382 246 5 5 380 4 374 2.90e-55 186
Msa0369880 AT4G30420.1 31.622 370 236 5 17 380 6 364 5.03e-54 183
Msa0369880 AT1G09380.1 28.455 369 245 4 10 366 8 369 1.69e-53 182
Msa0369880 AT3G30340.1 30.959 365 235 6 9 370 10 360 2.31e-52 178
Msa0369880 AT1G68170.2 29.805 359 244 3 6 357 9 366 3.83e-51 175
Msa0369880 AT1G68170.1 30.199 351 237 3 14 357 7 356 6.97e-51 174
Msa0369880 AT4G28040.1 31.915 329 208 4 9 335 7 321 1.16e-50 174
Msa0369880 AT4G28040.5 31.915 329 208 4 9 335 7 321 1.16e-50 174
Msa0369880 AT4G28040.3 31.915 329 208 4 9 335 7 321 1.16e-50 174
Msa0369880 AT4G28040.4 31.915 329 208 4 9 335 7 321 1.16e-50 174
Msa0369880 AT4G28040.2 31.915 329 208 4 9 335 7 321 1.16e-50 174
Msa0369880 AT3G53210.2 34.106 302 189 6 67 361 10 308 2.24e-49 169
Msa0369880 AT4G01440.1 31.511 311 210 2 30 340 30 337 2.28e-49 171
Msa0369880 AT1G01070.1 32.941 340 209 8 31 367 37 360 7.99e-49 169
Msa0369880 AT1G11450.3 30.836 347 226 6 10 353 16 351 8.81e-49 169
Msa0369880 AT1G11450.5 32.588 313 197 6 44 353 6 307 1.50e-46 162
Msa0369880 AT1G25270.1 30.663 362 230 6 9 357 2 355 1.12e-45 160
Msa0369880 AT3G18200.1 35.802 243 145 5 108 346 3 238 1.73e-45 157
Msa0369880 AT1G11450.2 32.258 310 196 6 47 353 2 300 5.75e-45 157
Msa0369880 AT3G28050.1 30.601 366 221 9 10 355 12 364 1.18e-44 158
Msa0369880 AT5G45370.2 31.157 337 214 6 17 337 27 361 2.45e-44 158
Msa0369880 AT5G45370.4 31.157 337 214 6 17 337 27 361 2.45e-44 158
Msa0369880 AT5G45370.1 30.793 328 211 4 17 341 27 341 3.15e-44 157
Msa0369880 AT1G43650.1 31.307 329 218 3 9 336 6 327 3.65e-44 156
Msa0369880 AT1G11460.1 31.098 328 216 5 10 333 16 337 9.83e-44 155
Msa0369880 AT3G28130.2 29.155 343 234 4 14 352 17 354 1.17e-43 155
Msa0369880 AT4G01430.1 28.889 360 241 6 12 366 13 362 8.90e-43 153
Msa0369880 AT3G28130.5 30.916 262 180 1 91 352 8 268 2.30e-41 147
Msa0369880 AT3G28130.1 30.916 262 180 1 91 352 8 268 2.30e-41 147
Msa0369880 AT5G40230.1 29.670 364 230 7 6 356 17 367 2.89e-41 149
Msa0369880 AT3G45870.1 29.141 326 220 5 9 325 11 334 3.37e-41 149
Msa0369880 AT4G01440.3 31.183 279 189 2 30 308 30 305 3.47e-41 148
Msa0369880 AT3G53210.3 33.992 253 163 4 6 257 6 255 1.33e-40 145
Msa0369880 AT5G40240.2 30.321 343 229 5 6 343 30 367 2.15e-40 147
Msa0369880 AT5G40240.1 30.321 343 229 5 6 343 16 353 2.58e-40 147
Msa0369880 AT5G40240.3 30.321 343 229 5 6 343 16 353 2.58e-40 147
Msa0369880 AT1G01070.2 31.429 315 197 8 56 367 15 313 5.01e-40 145
Msa0369880 AT4G19185.1 30.539 334 207 6 17 332 28 354 2.42e-39 145
Msa0369880 AT5G40210.1 28.696 345 221 8 14 355 17 339 3.64e-39 143
Msa0369880 AT3G28100.1 28.955 335 229 4 4 334 8 337 1.02e-38 142
Msa0369880 AT1G70260.1 26.027 365 258 5 10 365 12 373 2.15e-38 142
Msa0369880 AT5G64700.2 32.558 258 168 4 5 257 3 259 4.16e-38 138
Msa0369880 AT1G11450.4 30.464 302 201 4 10 308 16 311 1.05e-37 139
Msa0369880 AT1G11450.1 31.970 269 169 6 88 353 2 259 1.01e-36 134
Msa0369880 AT3G28080.1 28.955 335 229 4 4 334 8 337 4.12e-36 135
Msa0369880 AT3G28080.5 30.579 242 167 1 93 334 8 248 3.27e-35 130
Msa0369880 AT3G28080.7 30.579 242 167 1 93 334 8 248 3.27e-35 130
Msa0369880 AT3G28080.8 30.579 242 167 1 93 334 8 248 3.27e-35 130
Msa0369880 AT3G28080.6 30.579 242 167 1 93 334 8 248 3.27e-35 130
Msa0369880 AT5G47470.2 30.938 320 209 5 14 330 62 372 6.17e-35 133
Msa0369880 AT5G47470.1 30.938 320 209 5 14 330 34 344 7.15e-35 132
Msa0369880 AT5G47470.3 30.938 320 209 5 14 330 62 372 7.76e-35 132
Msa0369880 AT3G45870.8 28.155 309 211 5 9 308 11 317 9.19e-35 131
Msa0369880 AT3G28070.1 27.966 354 245 5 4 352 11 359 1.19e-34 131
Msa0369880 AT3G28070.2 29.502 261 182 2 93 352 8 267 1.71e-34 129
Msa0369880 AT3G28080.2 30.579 242 167 1 93 334 89 329 3.53e-34 130
Msa0369880 AT4G01450.2 32.166 314 204 3 30 342 30 335 4.30e-34 130
Msa0369880 AT3G28130.3 31.304 230 157 1 123 352 1 229 2.62e-33 124
Msa0369880 AT4G15540.1 28.571 350 227 6 6 347 13 347 6.08e-33 126
Msa0369880 AT4G15540.2 28.571 350 227 6 6 347 13 347 6.08e-33 126
Msa0369880 AT4G01430.2 29.000 300 206 4 12 308 13 308 2.21e-32 124
Msa0369880 AT3G30340.2 30.566 265 174 5 9 270 10 267 3.27e-32 123
Msa0369880 AT4G01440.5 30.303 231 158 2 110 340 2 229 3.96e-32 122
Msa0369880 AT4G01440.2 30.303 231 158 2 110 340 2 229 3.96e-32 122
Msa0369880 AT4G01440.4 32.075 212 141 2 35 246 35 243 1.01e-31 121
Msa0369880 AT4G01450.1 31.429 280 183 3 30 308 30 301 1.12e-31 123
Msa0369880 AT4G19185.2 30.303 264 159 6 87 332 20 276 3.34e-30 118
Msa0369880 AT4G01450.4 32.701 211 134 2 30 239 30 233 1.98e-29 115
Msa0369880 AT5G45370.6 30.268 261 164 6 93 337 1 259 2.83e-29 115
Msa0369880 AT5G45370.5 30.268 261 164 6 93 337 1 259 2.83e-29 115
Msa0369880 AT1G60050.1 24.332 374 256 7 10 368 13 374 3.75e-29 117
Msa0369880 AT3G28080.9 30.189 212 147 1 123 334 1 211 1.15e-27 109
Msa0369880 AT4G16620.1 28.483 323 217 6 35 352 37 350 1.19e-27 112
Msa0369880 AT4G01450.3 34.043 188 116 2 30 216 30 210 5.20e-27 108
Msa0369880 AT3G45870.9 28.302 265 175 6 3 258 9 267 1.31e-26 107
Msa0369880 AT3G28070.5 30.189 212 147 1 123 334 1 211 1.42e-26 106
Msa0369880 AT4G24980.1 26.384 307 157 4 24 330 1 238 6.59e-26 105
Msa0369880 AT3G45870.5 29.224 219 144 5 116 325 43 259 1.71e-24 102
Msa0369880 AT3G28070.3 26.861 309 217 4 4 308 11 314 5.45e-24 101
Msa0369880 AT3G28130.4 28.395 243 166 3 14 252 17 255 8.31e-24 99.8
Msa0369880 AT5G45370.3 30.213 235 146 6 124 342 74 306 9.81e-23 98.2
Msa0369880 AT1G43650.2 30.579 242 160 3 17 257 14 248 1.74e-22 96.3
Msa0369880 AT3G28080.3 25.970 335 185 6 4 334 8 283 2.38e-21 94.0
Msa0369880 AT4G01430.3 27.039 233 158 4 138 366 1 225 4.92e-21 91.3
Msa0369880 AT3G28100.3 27.668 253 175 3 4 252 8 256 8.55e-21 91.7
Msa0369880 AT4G16620.4 29.008 262 173 5 95 352 1 253 1.57e-20 90.5
Msa0369880 AT4G16620.2 29.008 262 173 5 95 352 1 253 1.57e-20 90.5
Msa0369880 AT3G28080.4 26.446 242 123 3 93 334 8 194 2.94e-20 89.0
Msa0369880 AT3G45870.2 33.333 144 94 2 182 325 42 183 3.68e-19 86.3
Msa0369880 AT3G45870.6 27.723 202 135 5 116 308 43 242 2.70e-18 84.0
Msa0369880 AT3G45870.7 27.723 202 135 5 116 308 43 242 2.70e-18 84.0
Msa0369880 AT4G16620.3 31.472 197 129 5 40 234 41 233 7.00e-17 80.5
Msa0369880 AT3G28100.2 28.251 223 152 3 4 222 8 226 9.90e-16 76.6
Msa0369880 AT3G28130.6 29.231 195 130 3 14 204 17 207 2.76e-15 74.7

Find 108 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCATTTGTTAGAAATTT+AGG 0.159464 3_1:-67235729 Msa0369880:three_prime_UTR
ATTTAATCAGCATATATTTA+AGG 0.170209 3_1:+67235646 None:intergenic
ATATCGTTACTTTCATTTCT+AGG 0.186256 3_1:+67236098 None:intergenic
TGTCATTATCTCTTTGAAAA+TGG 0.218801 3_1:+67235973 None:intergenic
CTATTGTTGGCTGTTCAATT+TGG 0.229434 3_1:-67238024 Msa0369880:CDS
AACTCTCATGCTTGGGCTCT+TGG 0.264844 3_1:-67236721 Msa0369880:CDS
TGATCAATGCTTTGCTCTGC+TGG 0.278960 3_1:-67237529 Msa0369880:CDS
AGGTCTGGTTATTAAAACTA+TGG 0.286351 3_1:-67236457 Msa0369880:CDS
TTGTTGGGAGTATTCATTAA+AGG 0.292892 3_1:-67235857 Msa0369880:three_prime_UTR
TGTTACTAGAAAGAGGAATT+TGG 0.305169 3_1:-67235924 Msa0369880:three_prime_UTR
GAAAGAGGAATTTGGCCATT+TGG 0.314426 3_1:-67235916 Msa0369880:three_prime_UTR
GCAACTTGGGTTTGTTTCAT+TGG 0.317703 3_1:-67236787 Msa0369880:CDS
TAGAATCACAAACCACAAAA+TGG 0.322143 3_1:+67235804 None:intergenic
ATCGGCACCATTGTAACATT+TGG 0.331344 3_1:-67237142 Msa0369880:CDS
CTAATTGCTCATTTAATTAG+TGG 0.336095 3_1:-67235884 Msa0369880:three_prime_UTR
ACTGGTGACCGTTGGATCAT+AGG 0.340097 3_1:-67237019 Msa0369880:CDS
TTCCTCCTCATTGGTTGTGC+TGG 0.340717 3_1:-67236989 Msa0369880:CDS
TTAAAAGCAGTTACAATAAC+TGG 0.347274 3_1:+67236432 None:intergenic
CTGATTAAATAATATTGTTA+AGG 0.361952 3_1:-67235633 Msa0369880:three_prime_UTR
GGAGCAATAGTGGTTGTTCT+TGG 0.362139 3_1:-67236245 Msa0369880:CDS
CTCATGCTTGGGCTCTTGGT+TGG 0.362607 3_1:-67236717 Msa0369880:CDS
GCTGTTCAATTTGGTTCTGC+TGG 0.366885 3_1:-67238015 Msa0369880:CDS
TCATGCTTGGGCTCTTGGTT+GGG 0.371511 3_1:-67236716 Msa0369880:CDS
CGATATTAATGAAGCTCAAT+TGG 0.371801 3_1:-67236081 Msa0369880:CDS
TTTAATTAGTGGTCACTTGT+TGG 0.384355 3_1:-67235873 Msa0369880:three_prime_UTR
TGCAATGAGTCCTCCTTTGT+AGG 0.384765 3_1:+67236152 None:intergenic
ACTGGAACACATGAAGATTA+AGG 0.386490 3_1:-67237188 Msa0369880:CDS
AGCGCCACAACTCTCATGCT+TGG 0.393575 3_1:-67236729 Msa0369880:CDS
GGGACGTGTTTCCTCCTCAT+TGG 0.398433 3_1:-67236998 Msa0369880:CDS
CACCAATGTCTCTAGCAACT+TGG 0.407752 3_1:-67236801 Msa0369880:CDS
ACAGCCAACAATAGTAGATA+TGG 0.410774 3_1:+67238033 None:intergenic
GATCAATGCTTTGCTCTGCT+GGG 0.413981 3_1:-67237528 Msa0369880:CDS
CAGTATTATTGGAGCAATAG+TGG 0.418656 3_1:-67236255 Msa0369880:CDS
TTAATTAGTGGTCACTTGTT+GGG 0.419073 3_1:-67235872 Msa0369880:three_prime_UTR
TATGATCATCGTAACGGCCT+TGG 0.421783 3_1:-67236400 Msa0369880:CDS
ATTTGGAGGAACATTGCTAA+TGG 0.425413 3_1:-67237125 Msa0369880:CDS
GAAACACCAACCGCGTAAGC+TGG 0.432985 3_1:+67236676 None:intergenic
GGTAAACAATGAAAACATAA+TGG 0.443753 3_1:+67237950 None:intergenic
TGGACTTTATCTAGTTGTGT+GGG 0.445412 3_1:-67236225 Msa0369880:CDS
TAAAAGAGAGACTCAAAAGA+TGG 0.446644 3_1:-67238079 Msa0369880:exon
TAACCGGTAGCTGCTGTTGT+TGG 0.446707 3_1:+67236126 None:intergenic
GTAAAAGAAGATGTAATAGA+TGG 0.452324 3_1:+67235752 None:intergenic
AAAACCATATCTACTATTGT+TGG 0.456596 3_1:-67238037 Msa0369880:CDS
TGGGTACTTTCTCAACGTTA+TGG 0.459781 3_1:+67236825 None:intergenic
GCCATGGATGCTATCAAGAA+AGG 0.462957 3_1:-67237979 Msa0369880:CDS
CTGGTGACCGTTGGATCATA+GGG 0.466455 3_1:-67237018 Msa0369880:CDS
AAACACCAACCGCGTAAGCT+GGG 0.467511 3_1:+67236677 None:intergenic
TCCTCCTTTGTAGGCGAGGA+TGG 0.474012 3_1:+67236161 None:intergenic
GCGCCACAACTCTCATGCTT+GGG 0.476115 3_1:-67236728 Msa0369880:CDS
AGGAATTTGGCCATTTGGCA+TGG 0.479726 3_1:-67235911 Msa0369880:three_prime_UTR
CTGGCGGGTAGCATGGCTAA+TGG 0.480297 3_1:+67237063 None:intergenic
GTTGCTAGAGACATTGGTGC+TGG 0.492665 3_1:+67236805 None:intergenic
TTACAGCAGCGATGGCATTA+CGG 0.493658 3_1:+67237929 None:intergenic
GCCTTTCTTGATAGCATCCA+TGG 0.499872 3_1:+67237978 None:intergenic
TTACAGGGGATAGTTACATC+AGG 0.501416 3_1:-67236498 Msa0369880:intron
TTGTATAAACAGACGTTGAT+CGG 0.507901 3_1:-67236001 Msa0369880:three_prime_UTR
TCATTTCTAGGAGCTGTAAC+CGG 0.508308 3_1:+67236110 None:intergenic
GCAGTATTACATACAAGGTC+TGG 0.510999 3_1:-67236472 Msa0369880:CDS
GACAGAAATTAAATGTGTCT+TGG 0.512162 3_1:-67236026 Msa0369880:exon
TTGGACTTTATCTAGTTGTG+TGG 0.512670 3_1:-67236226 Msa0369880:CDS
GGCACCATTGTAACATTTGG+AGG 0.513427 3_1:-67237139 Msa0369880:CDS
ACCAATGTCTCTAGCAACTT+GGG 0.527259 3_1:-67236800 Msa0369880:CDS
ATCAATGCTTTGCTCTGCTG+GGG 0.531569 3_1:-67237527 Msa0369880:CDS
ATGCATGTGTTACTAGAAAG+AGG 0.534032 3_1:-67235931 Msa0369880:three_prime_UTR
CCTTCGCATTTCATCTCGAA+AGG 0.535769 3_1:-67237897 Msa0369880:intron
AATTAGTGCTCCATGCCAAA+TGG 0.537015 3_1:+67235901 None:intergenic
TTTCAGGCTGGCGGGTAGCA+TGG 0.537593 3_1:+67237056 None:intergenic
AATACACGGACAGACATCTT+CGG 0.538912 3_1:+67237719 None:intergenic
GACCTCACAACACTAACTAC+AGG 0.541490 3_1:+67237088 None:intergenic
TGGCGCACTTCAAAGCTTCG+TGG 0.550157 3_1:-67236767 Msa0369880:CDS
CACAAAAGTAACAGAGGGCG+CGG 0.556408 3_1:+67237461 None:intergenic
GGTCTGGTTATTAAAACTAT+GGG 0.558618 3_1:-67236456 Msa0369880:CDS
CGCAAACACAAAAGTAACAG+AGG 0.561929 3_1:+67237455 None:intergenic
AGCTAACGTTACAGCAGCGA+TGG 0.569797 3_1:+67237921 None:intergenic
GCAATGTGAAATTGCTAATG+TGG 0.571756 3_1:+67238118 None:intergenic
ACTCTTTGCCCCAGCTTACG+CGG 0.585372 3_1:-67236686 Msa0369880:intron
AAAAGAGAGACTCAAAAGAT+GGG 0.585764 3_1:-67238078 Msa0369880:exon
GCGACCCCACTGGTGACCGT+TGG 0.587738 3_1:-67237027 Msa0369880:CDS
GGACTTTATCTAGTTGTGTG+GGG 0.592458 3_1:-67236224 Msa0369880:CDS
ATGATCCAACGGTCACCAGT+GGG 0.594849 3_1:+67237022 None:intergenic
ACCCAAGTTGCTAGAGACAT+TGG 0.595554 3_1:+67236799 None:intergenic
AGACCAACAACAGCAGCTAC+CGG 0.597120 3_1:-67236129 Msa0369880:CDS
GAGCCCAAGCATGAGAGTTG+TGG 0.598586 3_1:+67236725 None:intergenic
TGAGTCCTCCTTTGTAGGCG+AGG 0.599505 3_1:+67236157 None:intergenic
AACACCAACCGCGTAAGCTG+GGG 0.602908 3_1:+67236678 None:intergenic
CCTTTCGAGATGAAATGCGA+AGG 0.605006 3_1:+67237897 None:intergenic
GCCATCCTCGCCTACAAAGG+AGG 0.610670 3_1:-67236162 Msa0369880:CDS
TATGCCATCCTCGCCTACAA+AGG 0.610984 3_1:-67236165 Msa0369880:CDS
GGAGTGCAGTATTACATACA+AGG 0.611210 3_1:-67236477 Msa0369880:CDS
AGCCAGCACAACCAATGAGG+AGG 0.614566 3_1:+67236987 None:intergenic
AAAAGCCAGCACAACCAATG+AGG 0.615770 3_1:+67236984 None:intergenic
TTGCTAGAGACATTGGTGCT+GGG 0.616972 3_1:+67236806 None:intergenic
TGTTCCTCCAAATGTTACAA+TGG 0.620453 3_1:+67237135 None:intergenic
GCAAACACAAAAGTAACAGA+GGG 0.623135 3_1:+67237456 None:intergenic
TTGCTAATGGCTCTGTACAA+AGG 0.625645 3_1:-67237112 Msa0369880:CDS
TATGATCCAACGGTCACCAG+TGG 0.627987 3_1:+67237021 None:intergenic
CTTTCGAGATGAAATGCGAA+GGG 0.629918 3_1:+67237898 None:intergenic
TTTCACATTGCTAAGCACCC+AGG 0.635549 3_1:-67238106 Msa0369880:five_prime_UTR
AGAGAGGATGATGCAAGCCA+AGG 0.637732 3_1:+67236383 None:intergenic
AACACGTCCCTATGATCCAA+CGG 0.648934 3_1:+67237011 None:intergenic
GTCCTGTAGTTAGTGTTGTG+AGG 0.655305 3_1:-67237090 Msa0369880:CDS
GTTACGATGATCATACGTAC+CGG 0.664727 3_1:+67236408 None:intergenic
AGACTCAAAAGATGGGTGCG+TGG 0.670730 3_1:-67238071 Msa0369880:exon
GAAAATGTGAGCGACCCCAC+TGG 0.674765 3_1:-67237037 Msa0369880:CDS
GGTACGTATGATCATCGTAA+CGG 0.700079 3_1:-67236406 Msa0369880:CDS
TACCTGCAATATGTAAAACG+CGG 0.708080 3_1:+67236961 None:intergenic
TGATCCAACGGTCACCAGTG+GGG 0.718208 3_1:+67237023 None:intergenic
CACAGCTGACAAGAACGACG+CGG 0.752986 3_1:+67237491 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATGATTAACATTTTAATT+AGG - chr3_1:67235969-67235988 Msa0369880:three_prime_UTR 10.0%
!!! ATTTTTATTTCATTTTGAAA+TGG - chr3_1:67236817-67236836 Msa0369880:CDS 10.0%
!!! TTTTTGCTTATATTTTGTTA+CGG - chr3_1:67235939-67235958 Msa0369880:three_prime_UTR 15.0%
!! GGTTAATGTAAAAAAATAAA+GGG + chr3_1:67236466-67236485 None:intergenic 15.0%
!! TGGTTAATGTAAAAAAATAA+AGG + chr3_1:67236467-67236486 None:intergenic 15.0%
!! ACAAAAAAAAATGTCTATAA+GGG - chr3_1:67236520-67236539 Msa0369880:intron 15.0%
!! ATTTAATCAGCATATATTTA+AGG + chr3_1:67238112-67238131 None:intergenic 15.0%
!! CTGATTAAATAATATTGTTA+AGG - chr3_1:67238122-67238141 Msa0369880:five_prime_UTR 15.0%
!!! TATTTGGTCTAAAATTTGAA+TGG + chr3_1:67235899-67235918 None:intergenic 20.0%
!! TCAAATAAAGTTAACGTATT+TGG + chr3_1:67235915-67235934 None:intergenic 20.0%
!!! AAATATGTGTTTCTATGTTT+TGG - chr3_1:67236000-67236019 Msa0369880:three_prime_UTR 20.0%
!!! ACATAATCTCTAAAAATACA+CGG + chr3_1:67236053-67236072 None:intergenic 20.0%
!!! ATTTTTAGAGATTATGTTAC+TGG - chr3_1:67236056-67236075 Msa0369880:CDS 20.0%
!! ATTTATAATGCAATACCTTT+CGG - chr3_1:67236142-67236161 Msa0369880:CDS 20.0%
!! AAAAAAATAAAGGGTTTAGA+GGG + chr3_1:67236457-67236476 None:intergenic 20.0%
!! TAAAAAAATAAAGGGTTTAG+AGG + chr3_1:67236458-67236477 None:intergenic 20.0%
!!! TTTCATTTTTTTTGATAAGC+TGG + chr3_1:67236497-67236516 None:intergenic 20.0%
!! CACAAAAAAAAATGTCTATA+AGG - chr3_1:67236519-67236538 Msa0369880:intron 20.0%
!! GGGATGTTAATTTATTAAAT+TGG - chr3_1:67236540-67236559 Msa0369880:intron 20.0%
!! TAAAAATCAATCACAACAAA+AGG + chr3_1:67237240-67237259 None:intergenic 20.0%
!!! TTTTATGATGACAGTATTAT+TGG - chr3_1:67237489-67237508 Msa0369880:CDS 20.0%
!!! ACTTCATTTGTTAGAAATTT+AGG - chr3_1:67238026-67238045 Msa0369880:CDS 20.0%
! AAAACCATATCTACTATTGT+TGG - chr3_1:67235718-67235737 Msa0369880:three_prime_UTR 25.0%
! GGTAAACAATGAAAACATAA+TGG + chr3_1:67235808-67235827 None:intergenic 25.0%
!! TCTAAAATTTGAATGGAAGA+GGG + chr3_1:67235892-67235911 None:intergenic 25.0%
! GTTAAATGAACCATATATTG+CGG - chr3_1:67236376-67236395 Msa0369880:CDS 25.0%
! ATTTATTAAATTGGACAGAC+TGG - chr3_1:67236549-67236568 Msa0369880:intron 25.0%
! GAAACATGTATGTATATGTT+TGG - chr3_1:67236885-67236904 Msa0369880:intron 25.0%
!! TGTATATGTTTGGTTTAATG+TGG - chr3_1:67236895-67236914 Msa0369880:intron 25.0%
!!! TTGTTGTGATTGATTTTTAC+AGG - chr3_1:67237241-67237260 Msa0369880:intron 25.0%
!!! TGTTGTGATTGATTTTTACA+GGG - chr3_1:67237242-67237261 Msa0369880:intron 25.0%
! TTAAAAGCAGTTACAATAAC+TGG + chr3_1:67237326-67237345 None:intergenic 25.0%
!!! TATTGTAACTGCTTTTAATC+CGG - chr3_1:67237328-67237347 Msa0369880:intron 25.0%
!!! ATTTTGAGCTTTGTATTCTT+TGG + chr3_1:67237562-67237581 None:intergenic 25.0%
!! ATATCGTTACTTTCATTTCT+AGG + chr3_1:67237660-67237679 None:intergenic 25.0%
!! TGTCATTATCTCTTTGAAAA+TGG + chr3_1:67237785-67237804 None:intergenic 25.0%
! CTAATTGCTCATTTAATTAG+TGG - chr3_1:67237871-67237890 Msa0369880:intron 25.0%
! GTAAAAGAAGATGTAATAGA+TGG + chr3_1:67238006-67238025 None:intergenic 25.0%
TAAAAGAGAGACTCAAAAGA+TGG - chr3_1:67235676-67235695 Msa0369880:three_prime_UTR 30.0%
AAAAGAGAGACTCAAAAGAT+GGG - chr3_1:67235677-67235696 Msa0369880:three_prime_UTR 30.0%
! GTCTAAAATTTGAATGGAAG+AGG + chr3_1:67235893-67235912 None:intergenic 30.0%
!!! TATGTTTTGGCAGGAAAATT+AGG - chr3_1:67236013-67236032 Msa0369880:three_prime_UTR 30.0%
! TTGCATTTTACCGCAATATA+TGG + chr3_1:67236389-67236408 None:intergenic 30.0%
TAACATTGCAACTACAATAG+TGG + chr3_1:67236422-67236441 None:intergenic 30.0%
GCATGTCAAGCAAAAATAAT+CGG - chr3_1:67236595-67236614 Msa0369880:intron 30.0%
! GTTTAATGTGGTGATTTTGT+AGG - chr3_1:67236907-67236926 Msa0369880:intron 30.0%
CTATGTATTCTAAACTCGAA+AGG + chr3_1:67237135-67237154 None:intergenic 30.0%
!! GTTGTGATTGATTTTTACAG+GGG - chr3_1:67237243-67237262 Msa0369880:intron 30.0%
AGGTCTGGTTATTAAAACTA+TGG - chr3_1:67237298-67237317 Msa0369880:intron 30.0%
GGTCTGGTTATTAAAACTAT+GGG - chr3_1:67237299-67237318 Msa0369880:intron 30.0%
CGATATTAATGAAGCTCAAT+TGG - chr3_1:67237674-67237693 Msa0369880:intron 30.0%
GACAGAAATTAAATGTGTCT+TGG - chr3_1:67237729-67237748 Msa0369880:CDS 30.0%
TTGTATAAACAGACGTTGAT+CGG - chr3_1:67237754-67237773 Msa0369880:intron 30.0%
TGTTACTAGAAAGAGGAATT+TGG - chr3_1:67237831-67237850 Msa0369880:intron 30.0%
TTTAATTAGTGGTCACTTGT+TGG - chr3_1:67237882-67237901 Msa0369880:intron 30.0%
TTAATTAGTGGTCACTTGTT+GGG - chr3_1:67237883-67237902 Msa0369880:intron 30.0%
!! TTGTTGGGAGTATTCATTAA+AGG - chr3_1:67237898-67237917 Msa0369880:CDS 30.0%
TAGAATCACAAACCACAAAA+TGG + chr3_1:67237954-67237973 None:intergenic 30.0%
GCAATGTGAAATTGCTAATG+TGG + chr3_1:67235640-67235659 None:intergenic 35.0%
ACAGCCAACAATAGTAGATA+TGG + chr3_1:67235725-67235744 None:intergenic 35.0%
! CTATTGTTGGCTGTTCAATT+TGG - chr3_1:67235731-67235750 Msa0369880:three_prime_UTR 35.0%
!! TGGTATGTTCATTTTTGCCA+TGG - chr3_1:67235760-67235779 Msa0369880:three_prime_UTR 35.0%
!!! ATGTGTTTCTATGTTTTGGC+AGG - chr3_1:67236004-67236023 Msa0369880:three_prime_UTR 35.0%
AGTTTCTTTCTTGTACCGAA+AGG + chr3_1:67236160-67236179 None:intergenic 35.0%
GCAAACACAAAAGTAACAGA+GGG + chr3_1:67236302-67236321 None:intergenic 35.0%
!!! CTCTGTTACTTTTGTGTTTG+CGG - chr3_1:67236301-67236320 Msa0369880:intron 35.0%
TTTGATAAGCTGGCATTTGA+TGG + chr3_1:67236487-67236506 None:intergenic 35.0%
ACTGGAACACATGAAGATTA+AGG - chr3_1:67236567-67236586 Msa0369880:intron 35.0%
TGTTCCTCCAAATGTTACAA+TGG + chr3_1:67236623-67236642 None:intergenic 35.0%
ATTTGGAGGAACATTGCTAA+TGG - chr3_1:67236630-67236649 Msa0369880:intron 35.0%
TACCTGCAATATGTAAAACG+CGG + chr3_1:67236797-67236816 None:intergenic 35.0%
AACTCGAAAGGTGAATTCTA+TGG + chr3_1:67237123-67237142 None:intergenic 35.0%
CAAGAAAAAGTTGTTCAGAG+AGG + chr3_1:67237391-67237410 None:intergenic 35.0%
!! CTCTCTGAACAACTTTTTCT+TGG - chr3_1:67237389-67237408 Msa0369880:intron 35.0%
!! CTGAACAACTTTTTCTTGGA+AGG - chr3_1:67237393-67237412 Msa0369880:intron 35.0%
CAGTATTATTGGAGCAATAG+TGG - chr3_1:67237500-67237519 Msa0369880:CDS 35.0%
TTGGACTTTATCTAGTTGTG+TGG - chr3_1:67237529-67237548 Msa0369880:CDS 35.0%
TGGACTTTATCTAGTTGTGT+GGG - chr3_1:67237530-67237549 Msa0369880:CDS 35.0%
! ATGCATGTGTTACTAGAAAG+AGG - chr3_1:67237824-67237843 Msa0369880:intron 35.0%
!! AAAAAGCGTGATCCATTTTG+TGG - chr3_1:67237939-67237958 Msa0369880:CDS 35.0%
! AGTCTCTCTTTTACTCTCCT+GGG + chr3_1:67235669-67235688 None:intergenic 40.0%
CTTTCGAGATGAAATGCGAA+GGG + chr3_1:67235860-67235879 None:intergenic 40.0%
AATACACGGACAGACATCTT+CGG + chr3_1:67236039-67236058 None:intergenic 40.0%
CGCAAACACAAAAGTAACAG+AGG + chr3_1:67236303-67236322 None:intergenic 40.0%
ATCGGCACCATTGTAACATT+TGG - chr3_1:67236613-67236632 Msa0369880:intron 40.0%
! TTGCTAATGGCTCTGTACAA+AGG - chr3_1:67236643-67236662 Msa0369880:intron 40.0%
! TTCCGCGTTTTACATATTGC+AGG - chr3_1:67236792-67236811 Msa0369880:CDS 40.0%
TGGGTACTTTCTCAACGTTA+TGG + chr3_1:67236933-67236952 None:intergenic 40.0%
ACCAATGTCTCTAGCAACTT+GGG - chr3_1:67236955-67236974 Msa0369880:intron 40.0%
! GCAACTTGGGTTTGTTTCAT+TGG - chr3_1:67236968-67236987 Msa0369880:CDS 40.0%
TTACAGGGGATAGTTACATC+AGG - chr3_1:67237257-67237276 Msa0369880:intron 40.0%
GGAGTGCAGTATTACATACA+AGG - chr3_1:67237278-67237297 Msa0369880:intron 40.0%
GCAGTATTACATACAAGGTC+TGG - chr3_1:67237283-67237302 Msa0369880:intron 40.0%
GTTACGATGATCATACGTAC+CGG + chr3_1:67237350-67237369 None:intergenic 40.0%
GGTACGTATGATCATCGTAA+CGG - chr3_1:67237349-67237368 Msa0369880:intron 40.0%
GGACTTTATCTAGTTGTGTG+GGG - chr3_1:67237531-67237550 Msa0369880:CDS 40.0%
! TCATTTCTAGGAGCTGTAAC+CGG + chr3_1:67237648-67237667 None:intergenic 40.0%
GAAAGAGGAATTTGGCCATT+TGG - chr3_1:67237839-67237858 Msa0369880:intron 40.0%
AATTAGTGCTCCATGCCAAA+TGG + chr3_1:67237857-67237876 None:intergenic 40.0%
TTTCACATTGCTAAGCACCC+AGG - chr3_1:67235649-67235668 Msa0369880:three_prime_UTR 45.0%
! GAGTCTCTCTTTTACTCTCC+TGG + chr3_1:67235670-67235689 None:intergenic 45.0%
!! GCTGTTCAATTTGGTTCTGC+TGG - chr3_1:67235740-67235759 Msa0369880:three_prime_UTR 45.0%
GCCATGGATGCTATCAAGAA+AGG - chr3_1:67235776-67235795 Msa0369880:three_prime_UTR 45.0%
! GCCTTTCTTGATAGCATCCA+TGG + chr3_1:67235780-67235799 None:intergenic 45.0%
TTACAGCAGCGATGGCATTA+CGG + chr3_1:67235829-67235848 None:intergenic 45.0%
CCTTTCGAGATGAAATGCGA+AGG + chr3_1:67235861-67235880 None:intergenic 45.0%
CCTTCGCATTTCATCTCGAA+AGG - chr3_1:67235858-67235877 Msa0369880:three_prime_UTR 45.0%
TGATCAATGCTTTGCTCTGC+TGG - chr3_1:67236226-67236245 Msa0369880:CDS 45.0%
GATCAATGCTTTGCTCTGCT+GGG - chr3_1:67236227-67236246 Msa0369880:CDS 45.0%
ATCAATGCTTTGCTCTGCTG+GGG - chr3_1:67236228-67236247 Msa0369880:CDS 45.0%
GGCACCATTGTAACATTTGG+AGG - chr3_1:67236616-67236635 Msa0369880:intron 45.0%
GTCCTGTAGTTAGTGTTGTG+AGG - chr3_1:67236665-67236684 Msa0369880:intron 45.0%
GACCTCACAACACTAACTAC+AGG + chr3_1:67236670-67236689 None:intergenic 45.0%
AACACGTCCCTATGATCCAA+CGG + chr3_1:67236747-67236766 None:intergenic 45.0%
AAAAGCCAGCACAACCAATG+AGG + chr3_1:67236774-67236793 None:intergenic 45.0%
! TTGCTAGAGACATTGGTGCT+GGG + chr3_1:67236952-67236971 None:intergenic 45.0%
CACCAATGTCTCTAGCAACT+TGG - chr3_1:67236954-67236973 Msa0369880:intron 45.0%
ACCCAAGTTGCTAGAGACAT+TGG + chr3_1:67236959-67236978 None:intergenic 45.0%
TTGTGTCCAAATAGCGCAAG+TGG + chr3_1:67237174-67237193 None:intergenic 45.0%
TATGATCATCGTAACGGCCT+TGG - chr3_1:67237355-67237374 Msa0369880:intron 45.0%
GGAGCAATAGTGGTTGTTCT+TGG - chr3_1:67237510-67237529 Msa0369880:CDS 45.0%
TGCAATGAGTCCTCCTTTGT+AGG + chr3_1:67237606-67237625 None:intergenic 45.0%
AGGAATTTGGCCATTTGGCA+TGG - chr3_1:67237844-67237863 Msa0369880:intron 45.0%
AGACTCAAAAGATGGGTGCG+TGG - chr3_1:67235684-67235703 Msa0369880:three_prime_UTR 50.0%
AGCTAACGTTACAGCAGCGA+TGG + chr3_1:67235837-67235856 None:intergenic 50.0%
CACAAAAGTAACAGAGGGCG+CGG + chr3_1:67236297-67236316 None:intergenic 50.0%
ATGATCCAACGGTCACCAGT+GGG + chr3_1:67236736-67236755 None:intergenic 50.0%
TATGATCCAACGGTCACCAG+TGG + chr3_1:67236737-67236756 None:intergenic 50.0%
ACTGGTGACCGTTGGATCAT+AGG - chr3_1:67236736-67236755 Msa0369880:CDS 50.0%
CTGGTGACCGTTGGATCATA+GGG - chr3_1:67236737-67236756 Msa0369880:CDS 50.0%
! TTCCTCCTCATTGGTTGTGC+TGG - chr3_1:67236766-67236785 Msa0369880:CDS 50.0%
GTTGCTAGAGACATTGGTGC+TGG + chr3_1:67236953-67236972 None:intergenic 50.0%
AACTCTCATGCTTGGGCTCT+TGG - chr3_1:67237034-67237053 Msa0369880:CDS 50.0%
TCATGCTTGGGCTCTTGGTT+GGG - chr3_1:67237039-67237058 Msa0369880:CDS 50.0%
AAACACCAACCGCGTAAGCT+GGG + chr3_1:67237081-67237100 None:intergenic 50.0%
CACCAACCACTTGCGCTATT+TGG - chr3_1:67237165-67237184 Msa0369880:CDS 50.0%
GTCCAAATAGCGCAAGTGGT+TGG + chr3_1:67237170-67237189 None:intergenic 50.0%
AGAGAGGATGATGCAAGCCA+AGG + chr3_1:67237375-67237394 None:intergenic 50.0%
TATGCCATCCTCGCCTACAA+AGG - chr3_1:67237590-67237609 Msa0369880:intron 50.0%
AGACCAACAACAGCAGCTAC+CGG - chr3_1:67237626-67237645 Msa0369880:intron 50.0%
TAACCGGTAGCTGCTGTTGT+TGG + chr3_1:67237632-67237651 None:intergenic 50.0%
CACAGCTGACAAGAACGACG+CGG + chr3_1:67236267-67236286 None:intergenic 55.0%
!! GCTCACATTTTCAGGCTGGC+GGG + chr3_1:67236710-67236729 None:intergenic 55.0%
!! CGCTCACATTTTCAGGCTGG+CGG + chr3_1:67236711-67236730 None:intergenic 55.0%
!! GGTCGCTCACATTTTCAGGC+TGG + chr3_1:67236714-67236733 None:intergenic 55.0%
! GTGGGGTCGCTCACATTTTC+AGG + chr3_1:67236718-67236737 None:intergenic 55.0%
GAAAATGTGAGCGACCCCAC+TGG - chr3_1:67236718-67236737 Msa0369880:CDS 55.0%
TGATCCAACGGTCACCAGTG+GGG + chr3_1:67236735-67236754 None:intergenic 55.0%
GGGACGTGTTTCCTCCTCAT+TGG - chr3_1:67236757-67236776 Msa0369880:CDS 55.0%
AGCCAGCACAACCAATGAGG+AGG + chr3_1:67236771-67236790 None:intergenic 55.0%
TGGCGCACTTCAAAGCTTCG+TGG - chr3_1:67236988-67237007 Msa0369880:CDS 55.0%
AGCGCCACAACTCTCATGCT+TGG - chr3_1:67237026-67237045 Msa0369880:CDS 55.0%
GCGCCACAACTCTCATGCTT+GGG - chr3_1:67237027-67237046 Msa0369880:CDS 55.0%
GAGCCCAAGCATGAGAGTTG+TGG + chr3_1:67237033-67237052 None:intergenic 55.0%
CTCATGCTTGGGCTCTTGGT+TGG - chr3_1:67237038-67237057 Msa0369880:CDS 55.0%
ACTCTTTGCCCCAGCTTACG+CGG - chr3_1:67237069-67237088 Msa0369880:CDS 55.0%
AACACCAACCGCGTAAGCTG+GGG + chr3_1:67237080-67237099 None:intergenic 55.0%
GAAACACCAACCGCGTAAGC+TGG + chr3_1:67237082-67237101 None:intergenic 55.0%
TCCTCCTTTGTAGGCGAGGA+TGG + chr3_1:67237597-67237616 None:intergenic 55.0%
TGAGTCCTCCTTTGTAGGCG+AGG + chr3_1:67237601-67237620 None:intergenic 55.0%
! CTGGCGGGTAGCATGGCTAA+TGG + chr3_1:67236695-67236714 None:intergenic 60.0%
! TTTCAGGCTGGCGGGTAGCA+TGG + chr3_1:67236702-67236721 None:intergenic 60.0%
TTTGCCCCAGCTTACGCGGT+TGG - chr3_1:67237073-67237092 Msa0369880:CDS 60.0%
GCCATCCTCGCCTACAAAGG+AGG - chr3_1:67237593-67237612 Msa0369880:intron 60.0%
GCGACCCCACTGGTGACCGT+TGG - chr3_1:67236728-67236747 Msa0369880:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 67235628 67238149 67235628 ID=Msa0369880;Name=Msa0369880
chr3_1 mRNA 67235628 67238149 67235628 ID=Msa0369880-mRNA-1;Parent=Msa0369880;Name=Msa0369880-mRNA-1;_AED=0.24;_eAED=0.24;_QI=67|1|1|1|1|1|7|409|387
chr3_1 exon 67235628 67236274 67235628 ID=Msa0369880-mRNA-1:exon:13846;Parent=Msa0369880-mRNA-1
chr3_1 exon 67236363 67236514 67236363 ID=Msa0369880-mRNA-1:exon:13845;Parent=Msa0369880-mRNA-1
chr3_1 exon 67236687 67236848 67236687 ID=Msa0369880-mRNA-1:exon:13844;Parent=Msa0369880-mRNA-1
chr3_1 exon 67236964 67237210 67236964 ID=Msa0369880-mRNA-1:exon:13843;Parent=Msa0369880-mRNA-1
chr3_1 exon 67237444 67237560 67237444 ID=Msa0369880-mRNA-1:exon:13842;Parent=Msa0369880-mRNA-1
chr3_1 exon 67237689 67237751 67237689 ID=Msa0369880-mRNA-1:exon:13841;Parent=Msa0369880-mRNA-1
chr3_1 exon 67237898 67238149 67237898 ID=Msa0369880-mRNA-1:exon:13840;Parent=Msa0369880-mRNA-1
chr3_1 five_prime_UTR 67238083 67238149 67238083 ID=Msa0369880-mRNA-1:five_prime_utr;Parent=Msa0369880-mRNA-1
chr3_1 CDS 67237898 67238082 67237898 ID=Msa0369880-mRNA-1:cds;Parent=Msa0369880-mRNA-1
chr3_1 CDS 67237689 67237751 67237689 ID=Msa0369880-mRNA-1:cds;Parent=Msa0369880-mRNA-1
chr3_1 CDS 67237444 67237560 67237444 ID=Msa0369880-mRNA-1:cds;Parent=Msa0369880-mRNA-1
chr3_1 CDS 67236964 67237210 67236964 ID=Msa0369880-mRNA-1:cds;Parent=Msa0369880-mRNA-1
chr3_1 CDS 67236687 67236848 67236687 ID=Msa0369880-mRNA-1:cds;Parent=Msa0369880-mRNA-1
chr3_1 CDS 67236363 67236514 67236363 ID=Msa0369880-mRNA-1:cds;Parent=Msa0369880-mRNA-1
chr3_1 CDS 67236037 67236274 67236037 ID=Msa0369880-mRNA-1:cds;Parent=Msa0369880-mRNA-1
chr3_1 three_prime_UTR 67235628 67236036 67235628 ID=Msa0369880-mRNA-1:three_prime_utr;Parent=Msa0369880-mRNA-1
Gene Sequence

>Msa0369880

ATGGGTGCGTGGTTGAAAAATGCAAAACCATATCTACTATTGTTGGCTGTTCAATTTGGTTCTGCTGGTATGTTCATTTTTGCCATGGATGCTATCAAGAAAGGCATGAGCCATTATGTTTTCATTGTTTACCGTAATGCCATCGCTGCTGTAACGTTAGCTCCCTTCGCATTTCATCTCGAAAGGAAAATTAGGCCGAAGATGTCTGTCCGTGTATTTTTAGAGATTATGTTACTGGCTTTCTTTGAAATAATACTTGATCAATGCTTTGCTCTGCTGGGGATGAAGTTAACGTCCGCGTCGTTCTTGTCAGCTGTGATGAACTCCGCGCCCTCTGTTACTTTTGTGTTTGCGGTCATTCTAAAACTGGAACACATGAAGATTAAGGAGATAGCATGTCAAGCAAAAATAATCGGCACCATTGTAACATTTGGAGGAACATTGCTAATGGCTCTGTACAAAGGTCCTGTAGTTAGTGTTGTGAGGTCATCCATTAGCCATGCTACCCGCCAGCCTGAAAATGTGAGCGACCCCACTGGTGACCGTTGGATCATAGGGACGTGTTTCCTCCTCATTGGTTGTGCTGGCTTTTCCGCGTTTTACATATTGCAGGCCATAACGTTGAGAAAGTACCCAGCACCAATGTCTCTAGCAACTTGGGTTTGTTTCATTGGCGCACTTCAAAGCTTCGTGGTAGCATTCTTCGCAGAGCGCCACAACTCTCATGCTTGGGCTCTTGGTTGGGATACAAGACTCTTTGCCCCAGCTTACGCGGGGATAGTTACATCAGGAGTGCAGTATTACATACAAGGTCTGGTTATTAAAACTATGGGACCAGTTATTGTAACTGCTTTTAATCCGGTACGTATGATCATCGTAACGGCCTTGGCTTGCATCATCCTCTCTGAACAACTTTTTCTTGGAAGTATTATTGGAGCAATAGTGGTTGTTCTTGGACTTTATCTAGTTGTGTGGGGAAAATCCAAAGAATACAAAGCTCAAAATCATATTGATATGCCATCCTCGCCTACAAAGGAGGACTCATTGCAAGACCAACAACAGCAGCTACCGGTTACAGCTCCTAGAAATGAAAGTAACGATATTAATGAAGCTCAATTGGTCATTCACAGTGAAGAAAAATGTGATGCTTGTGACAGAAATTAA
Protein sequence

>Msa0369880

MGAWLKNAKPYLLLLAVQFGSAGMFIFAMDAIKKGMSHYVFIVYRNAIAAVTLAPFAFHLERKIRPKMSVRVFLEIMLLAFFEIILDQCFALLGMKLTSASFLSAVMNSAPSVTFVFAVILKLEHMKIKEIACQAKIIGTIVTFGGTLLMALYKGPVVSVVRSSISHATRQPENVSDPTGDRWIIGTCFLLIGCAGFSAFYILQAITLRKYPAPMSLATWVCFIGALQSFVVAFFAERHNSHAWALGWDTRLFAPAYAGIVTSGVQYYIQGLVIKTMGPVIVTAFNPVRMIIVTALACIILSEQLFLGSIIGAIVVVLGLYLVVWGKSKEYKAQNHIDMPSSPTKEDSLQDQQQQLPVTAPRNESNDINEAQLVIHSEEKCDACDRN*