AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0509630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0509630 MtrunA17_Chr3g0114701 97.674 387 9 0 1 387 1 387 0.0 783
Msa0509630 MtrunA17_Chr1g0160181 66.755 379 121 2 1 379 10 383 0.0 511
Msa0509630 MtrunA17_Chr3g0114731 51.934 362 163 4 7 368 16 366 5.33e-134 389
Msa0509630 MtrunA17_Chr3g0114711 47.980 396 153 6 5 359 10 393 1.90e-124 364
Msa0509630 MtrunA17_Chr1g0177221 65.234 256 86 1 3 258 12 264 2.25e-119 347
Msa0509630 MtrunA17_Chr1g0160171 44.595 370 193 4 1 363 8 372 3.22e-111 330
Msa0509630 MtrunA17_Chr7g0255561 45.783 332 177 2 3 332 8 338 5.71e-109 325
Msa0509630 MtrunA17_Chr7g0255581 45.783 332 177 2 3 332 8 338 5.71e-109 325
Msa0509630 MtrunA17_Chr3g0114691 40.310 387 220 3 2 387 13 389 3.10e-108 323
Msa0509630 MtrunA17_Chr1g0182291 43.343 353 190 3 4 356 10 352 2.20e-106 317
Msa0509630 MtrunA17_Chr7g0263101 41.555 373 205 3 6 378 10 369 7.43e-105 314
Msa0509630 MtrunA17_Chr8g0354121 41.406 384 195 6 5 378 9 372 1.90e-99 301
Msa0509630 MtrunA17_Chr3g0081421 44.848 330 178 4 4 330 12 340 2.46e-99 300
Msa0509630 MtrunA17_Chr3g0081511 44.411 331 179 4 2 330 10 337 2.67e-96 293
Msa0509630 MtrunA17_Chr1g0186641 39.571 326 193 2 5 330 9 330 2.44e-80 250
Msa0509630 MtrunA17_Chr1g0186601 38.650 326 196 2 5 330 12 333 2.31e-78 245
Msa0509630 MtrunA17_Chr1g0186571 38.957 326 195 2 5 330 9 330 3.54e-77 242
Msa0509630 MtrunA17_Chr1g0186561 38.769 325 196 1 6 330 13 334 4.28e-76 239
Msa0509630 MtrunA17_Chr1g0186611 37.231 325 200 1 6 330 13 333 2.50e-75 238
Msa0509630 MtrunA17_Chr1g0171301 37.231 325 202 1 6 330 13 335 2.18e-74 235
Msa0509630 MtrunA17_Chr1g0155761 35.279 377 226 3 11 370 23 398 2.05e-71 229
Msa0509630 MtrunA17_Chr3g0142131 34.746 354 218 4 14 355 25 377 2.79e-69 223
Msa0509630 MtrunA17_Chr3g0129601 36.280 328 205 3 5 330 25 350 7.69e-69 221
Msa0509630 MtrunA17_Chr1g0184221 34.890 364 207 6 9 354 16 367 2.81e-68 220
Msa0509630 MtrunA17_Chr6g0463971 35.674 356 208 6 5 355 12 351 1.70e-66 215
Msa0509630 MtrunA17_Chr3g0129571 36.000 325 203 3 10 330 8 331 3.38e-65 211
Msa0509630 MtrunA17_Chr6g0483401 36.982 338 201 5 18 348 19 351 7.03e-65 211
Msa0509630 MtrunA17_Chr4g0050881 36.467 351 214 5 1 348 1 345 7.91e-64 207
Msa0509630 MtrunA17_Chr2g0278561 34.677 372 237 3 9 379 15 381 1.45e-63 208
Msa0509630 MtrunA17_Chr2g0284261 34.262 359 228 4 9 366 8 359 2.96e-61 201
Msa0509630 MtrunA17_Chr8g0383731 32.857 350 226 4 5 348 1 347 1.79e-60 199
Msa0509630 MtrunA17_Chr1g0164831 32.853 347 218 6 9 350 3 339 2.64e-60 198
Msa0509630 MtrunA17_Chr7g0271561 32.822 326 213 3 10 330 8 332 1.34e-59 197
Msa0509630 MtrunA17_Chr3g0129591 33.427 356 226 5 10 361 6 354 5.06e-59 195
Msa0509630 MtrunA17_Chr2g0278541 32.000 375 240 6 2 367 3 371 2.25e-58 194
Msa0509630 MtrunA17_Chr2g0278551 31.733 375 246 3 9 380 12 379 9.75e-58 193
Msa0509630 MtrunA17_Chr2g0290611 33.428 353 227 3 5 352 8 357 3.91e-57 191
Msa0509630 MtrunA17_Chr8g0334761 32.249 369 235 6 10 377 11 365 5.11e-57 190
Msa0509630 MtrunA17_Chr4g0051021 31.183 372 248 5 5 372 10 377 4.09e-55 186
Msa0509630 MtrunA17_Chr3g0126101 31.405 363 229 6 6 368 3 345 8.69e-55 184
Msa0509630 MtrunA17_Chr2g0332001 29.459 370 246 4 9 372 38 398 9.47e-55 185
Msa0509630 MtrunA17_Chr1g0172881 62.044 137 42 1 122 258 23 149 1.59e-54 177
Msa0509630 MtrunA17_Chr2g0310441 34.568 324 204 5 10 330 11 329 2.78e-53 181
Msa0509630 MtrunA17_Chr4g0059311 30.286 350 239 4 4 351 6 352 3.99e-52 177
Msa0509630 MtrunA17_Chr4g0050891 32.407 324 215 3 5 325 9 331 2.02e-51 176
Msa0509630 MtrunA17_Chr3g0129581 32.493 357 232 5 10 361 6 358 3.81e-51 175
Msa0509630 MtrunA17_Chr4g0050941 33.923 339 217 4 5 337 8 345 9.33e-51 174
Msa0509630 MtrunA17_Chr5g0413951 31.447 318 213 4 14 330 12 325 1.25e-49 171
Msa0509630 MtrunA17_Chr5g0413941 30.247 324 221 4 14 336 12 331 2.03e-49 170
Msa0509630 MtrunA17_Chr8g0334751 33.811 349 225 4 10 357 12 355 1.02e-48 168
Msa0509630 MtrunA17_Chr8g0334771 33.851 322 209 3 10 330 11 329 1.28e-48 169
Msa0509630 MtrunA17_Chr1g0160111 27.920 351 238 6 18 356 16 363 1.37e-48 168
Msa0509630 MtrunA17_Chr5g0407971 30.387 362 234 6 9 356 11 368 2.32e-48 168
Msa0509630 MtrunA17_Chr5g0413961 31.073 354 218 8 10 358 15 347 2.49e-47 164
Msa0509630 MtrunA17_Chr5g0408001 29.275 386 246 8 3 367 8 387 9.03e-47 164
Msa0509630 MtrunA17_Chr5g0408031 29.737 380 232 7 6 366 7 370 2.18e-46 163
Msa0509630 MtrunA17_Chr4g0073241 27.855 359 248 6 10 364 9 360 2.10e-45 160
Msa0509630 MtrunA17_Chr5g0408041 29.722 360 236 4 9 353 11 368 2.22e-45 160
Msa0509630 MtrunA17_Chr5g0413921 30.091 329 225 4 14 341 12 336 8.72e-45 157
Msa0509630 MtrunA17_Chr5g0407991 29.211 380 243 7 9 369 9 381 9.72e-44 156
Msa0509630 MtrunA17_Chr7g0227921 29.738 343 222 6 31 372 33 357 2.49e-43 154
Msa0509630 MtrunA17_Chr5g0428881 27.467 375 267 4 1 372 10 382 1.28e-42 153
Msa0509630 MtrunA17_Chr5g0413931 30.357 336 223 8 13 344 10 338 1.72e-42 152
Msa0509630 MtrunA17_Chr8g0369841 28.409 352 234 6 17 353 17 365 2.94e-42 152
Msa0509630 MtrunA17_Chr1g0150151 27.829 327 232 3 6 330 8 332 3.29e-41 149
Msa0509630 MtrunA17_Chr4g0050931 28.080 349 206 6 5 348 8 316 1.33e-39 143
Msa0509630 MtrunA17_Chr5g0404811 30.736 231 157 2 122 352 6 233 1.82e-35 130
Msa0509630 MtrunA17_Chr1g0172891 56.452 124 42 3 252 375 1 112 2.10e-35 126
Msa0509630 MtrunA17_Chr4g0051001 28.617 311 208 3 5 308 27 330 3.67e-35 132
Msa0509630 MtrunA17_Chr6g0451171 28.655 342 231 7 10 348 13 344 7.98e-35 131
Msa0509630 MtrunA17_Chr3g0126091 26.000 350 240 7 10 352 12 349 2.12e-34 130
Msa0509630 MtrunA17_Chr6g0451201 30.818 318 211 6 31 348 34 342 2.37e-34 130
Msa0509630 MtrunA17_Chr5g0441761 26.923 338 215 5 23 360 40 345 8.28e-34 128
Msa0509630 MtrunA17_Chr1g0177231 56.522 115 36 3 251 362 18 121 1.26e-33 121
Msa0509630 MtrunA17_Chr2g0278021 37.705 183 105 4 164 346 54 227 1.85e-32 123
Msa0509630 MtrunA17_Chr1g0182261 43.165 139 77 2 198 334 76 214 8.01e-32 120
Msa0509630 MtrunA17_Chr6g0451251 30.312 320 214 5 31 348 34 346 2.66e-30 119
Msa0509630 MtrunA17_Chr6g0451211 26.791 321 225 7 31 348 34 347 2.79e-30 119
Msa0509630 MtrunA17_Chr5g0429001 26.269 335 195 6 61 367 3 313 8.30e-30 117
Msa0509630 MtrunA17_Chr3g0084091 27.698 278 195 4 94 368 15 289 1.02e-29 116
Msa0509630 MtrunA17_Chr1g0211801 29.880 251 170 4 9 258 11 256 3.08e-29 114
Msa0509630 MtrunA17_Chr6g0451261 26.059 307 222 4 6 311 8 310 1.25e-28 118
Msa0509630 MtrunA17_Chr6g0451191 29.675 246 164 5 123 368 1 237 1.50e-28 111
Msa0509630 MtrunA17_Chr4g0056081 28.746 327 215 8 14 330 19 337 6.92e-27 109
Msa0509630 MtrunA17_Chr5g0399281 29.179 329 208 8 14 330 13 328 7.71e-25 103
Msa0509630 MtrunA17_Chr8g0334711 34.694 196 124 3 10 204 11 203 8.50e-24 98.2
Msa0509630 MtrunA17_Chr8g0334721 40.476 126 74 1 221 346 1 125 3.34e-22 92.4
Msa0509630 MtrunA17_Chr5g0404801 41.880 117 68 0 6 122 4 120 1.06e-19 84.3
Msa0509630 MtrunA17_Chr2g0278031 36.752 117 74 0 5 121 10 126 9.60e-13 64.7
Msa0509630 MtrunA17_Chr4g0050921 32.039 103 69 1 209 311 3 104 1.29e-12 64.7
Msa0509630 MtrunA17_Chr4g0021721 55.556 45 20 0 238 282 2 46 1.40e-12 62.4
Msa0509630 MtrunA17_Chr8g0372571 55.556 45 20 0 238 282 2 46 1.43e-12 62.8
Msa0509630 MtrunA17_Chr1g0211811 40.000 75 45 0 260 334 1 75 3.83e-12 62.0
Msa0509630 MtrunA17_Chr8g0334731 30.000 150 100 3 58 204 40 187 1.66e-11 63.2
Msa0509630 MtrunA17_Chr6g0451241 37.333 75 46 1 274 348 23 96 6.40e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0509630 AT4G08290.1 47.778 360 178 3 9 368 12 361 1.72e-121 357
Msa0509630 AT2G39510.1 43.915 378 190 5 5 375 3 365 6.25e-105 314
Msa0509630 AT2G37460.1 40.107 374 210 2 5 378 10 369 9.92e-105 314
Msa0509630 AT1G21890.1 45.058 344 180 3 1 336 1 343 1.87e-101 306
Msa0509630 AT1G44800.1 40.860 372 205 5 2 371 3 361 5.26e-100 301
Msa0509630 AT4G08300.1 39.295 369 220 3 2 367 3 370 3.83e-94 286
Msa0509630 AT5G07050.1 41.018 334 190 3 3 330 11 343 1.47e-93 286
Msa0509630 AT2G40900.1 41.441 333 187 3 6 338 7 331 5.12e-93 285
Msa0509630 AT1G21890.2 45.253 316 164 3 1 308 1 315 1.43e-89 274
Msa0509630 AT4G08290.2 49.398 249 125 1 9 257 12 259 4.42e-86 263
Msa0509630 AT3G56620.1 40.867 323 182 3 6 327 7 321 1.35e-84 262
Msa0509630 AT2G37450.2 40.782 358 176 5 3 355 2 328 2.09e-83 258
Msa0509630 AT5G13670.1 36.339 366 214 5 6 364 4 357 6.31e-78 245
Msa0509630 AT1G75500.1 37.994 329 194 5 11 332 21 346 4.03e-71 228
Msa0509630 AT1G75500.2 37.994 329 194 5 11 332 21 346 4.03e-71 228
Msa0509630 AT3G18200.2 34.375 352 223 4 6 355 31 376 1.87e-66 216
Msa0509630 AT2G37450.3 42.857 266 137 3 95 355 1 256 6.49e-65 208
Msa0509630 AT3G53210.1 34.711 363 227 6 6 361 6 365 1.49e-64 210
Msa0509630 AT5G64700.1 32.804 378 225 7 5 375 3 358 1.70e-61 202
Msa0509630 AT4G30420.2 31.989 372 237 4 5 373 4 362 2.91e-57 192
Msa0509630 AT4G30420.1 32.500 360 227 4 17 373 6 352 7.90e-56 188
Msa0509630 AT2G37450.1 43.860 228 118 2 122 349 61 278 4.47e-55 184
Msa0509630 AT2G37450.1 46.667 60 32 0 3 62 2 61 5.32e-11 63.5
Msa0509630 AT1G09380.1 28.455 369 245 4 10 366 8 369 1.52e-52 179
Msa0509630 AT1G68170.2 30.084 359 247 3 6 361 9 366 4.68e-52 177
Msa0509630 AT1G68170.1 30.484 351 240 3 14 361 7 356 1.16e-51 176
Msa0509630 AT3G30340.1 31.304 345 221 5 9 350 10 341 6.58e-51 174
Msa0509630 AT3G53210.2 34.395 314 190 8 56 361 3 308 3.26e-50 171
Msa0509630 AT4G28040.1 30.659 349 220 5 9 355 7 335 8.68e-50 171
Msa0509630 AT4G28040.5 30.659 349 220 5 9 355 7 335 8.68e-50 171
Msa0509630 AT4G28040.3 30.659 349 220 5 9 355 7 335 8.68e-50 171
Msa0509630 AT4G28040.4 30.659 349 220 5 9 355 7 335 8.68e-50 171
Msa0509630 AT4G28040.2 30.659 349 220 5 9 355 7 335 8.68e-50 171
Msa0509630 AT4G01440.1 31.190 311 211 2 30 340 30 337 2.44e-48 168
Msa0509630 AT1G11450.3 32.110 327 213 4 10 333 16 336 3.93e-48 167
Msa0509630 AT1G01070.1 32.647 340 210 8 31 367 37 360 6.76e-48 167
Msa0509630 AT1G11450.5 34.130 293 184 4 44 333 6 292 8.35e-46 160
Msa0509630 AT3G18200.1 34.800 250 155 4 108 355 3 246 1.69e-45 157
Msa0509630 AT3G28050.1 32.317 328 202 7 10 330 12 326 1.57e-44 158
Msa0509630 AT1G25270.1 30.874 366 224 8 9 357 2 355 1.62e-44 157
Msa0509630 AT5G45370.2 31.307 329 211 5 17 332 27 353 2.37e-44 158
Msa0509630 AT5G45370.4 31.307 329 211 5 17 332 27 353 2.37e-44 158
Msa0509630 AT5G45370.1 31.013 316 205 3 17 332 27 329 3.21e-44 157
Msa0509630 AT1G11450.2 33.793 290 183 4 47 333 2 285 3.31e-44 155
Msa0509630 AT1G11460.1 31.402 328 215 5 10 333 16 337 1.75e-43 154
Msa0509630 AT3G28130.2 29.155 343 234 4 14 352 17 354 1.61e-42 152
Msa0509630 AT1G43650.1 30.395 329 221 3 9 336 6 327 1.71e-42 152
Msa0509630 AT4G01430.1 28.852 357 240 6 12 358 13 365 1.81e-42 152
Msa0509630 AT3G45870.1 28.532 361 245 6 9 358 11 369 2.01e-41 150
Msa0509630 AT4G01440.3 30.824 279 190 2 30 308 30 305 1.53e-40 146
Msa0509630 AT5G40230.1 29.670 364 230 7 6 356 17 367 2.28e-40 147
Msa0509630 AT3G53210.3 34.387 253 162 4 6 257 6 255 2.40e-40 144
Msa0509630 AT3G28130.5 30.534 262 181 1 91 352 8 268 2.74e-40 144
Msa0509630 AT3G28130.1 30.534 262 181 1 91 352 8 268 2.74e-40 144
Msa0509630 AT4G19185.1 30.838 334 206 6 17 332 28 354 7.18e-40 146
Msa0509630 AT5G40240.2 30.321 343 229 5 6 343 30 367 1.20e-39 145
Msa0509630 AT3G28100.1 29.730 333 225 4 4 332 8 335 1.32e-39 144
Msa0509630 AT5G40240.1 30.321 343 229 5 6 343 16 353 1.48e-39 145
Msa0509630 AT5G40240.3 30.321 343 229 5 6 343 16 353 1.48e-39 145
Msa0509630 AT1G01070.2 31.111 315 198 8 56 367 15 313 4.66e-39 142
Msa0509630 AT1G70260.1 26.703 367 253 6 10 365 12 373 1.12e-38 142
Msa0509630 AT5G40210.1 28.406 345 222 8 14 355 17 339 2.17e-38 141
Msa0509630 AT5G64700.2 32.558 258 168 4 5 257 3 259 9.31e-38 137
Msa0509630 AT1G11450.4 30.795 302 200 4 10 308 16 311 1.77e-37 138
Msa0509630 AT3G28080.1 29.552 335 227 4 4 334 8 337 4.02e-37 138
Msa0509630 AT1G11450.1 33.735 249 156 4 88 333 2 244 4.75e-36 132
Msa0509630 AT5G47470.3 30.938 320 209 5 14 330 62 372 7.80e-36 135
Msa0509630 AT5G47470.1 30.938 320 209 5 14 330 34 344 8.57e-36 134
Msa0509630 AT5G47470.2 30.938 320 209 5 14 330 62 372 9.07e-36 135
Msa0509630 AT3G28080.5 30.992 242 166 1 93 334 8 248 2.00e-35 131
Msa0509630 AT3G28080.7 30.992 242 166 1 93 334 8 248 2.00e-35 131
Msa0509630 AT3G28080.8 30.992 242 166 1 93 334 8 248 2.00e-35 131
Msa0509630 AT3G28080.6 30.992 242 166 1 93 334 8 248 2.00e-35 131
Msa0509630 AT3G45870.8 28.155 309 211 5 9 308 11 317 7.41e-35 131
Msa0509630 AT3G28080.2 30.483 269 179 3 69 334 66 329 1.37e-34 131
Msa0509630 AT3G28070.1 28.060 335 232 4 4 334 11 340 5.23e-34 129
Msa0509630 AT4G01450.2 31.847 314 205 3 30 342 30 335 1.24e-33 129
Msa0509630 AT3G28070.2 29.752 242 169 1 93 334 8 248 2.26e-33 125
Msa0509630 AT3G28130.3 30.870 230 158 1 123 352 1 229 1.56e-32 122
Msa0509630 AT4G01430.2 28.667 300 207 4 12 308 13 308 3.10e-32 124
Msa0509630 AT3G30340.2 30.830 253 167 4 9 258 10 257 8.95e-32 122
Msa0509630 AT4G01450.1 31.071 280 184 3 30 308 30 301 1.51e-31 122
Msa0509630 AT4G15540.1 28.000 350 229 6 6 347 13 347 2.57e-31 122
Msa0509630 AT4G15540.2 28.000 350 229 6 6 347 13 347 2.57e-31 122
Msa0509630 AT4G01440.5 29.437 231 160 2 110 340 2 229 2.78e-31 120
Msa0509630 AT4G01440.2 29.437 231 160 2 110 340 2 229 2.78e-31 120
Msa0509630 AT4G01440.4 31.250 224 151 2 35 258 35 255 3.32e-31 120
Msa0509630 AT4G19185.2 30.714 280 168 7 87 347 20 292 7.80e-31 120
Msa0509630 AT5G45370.6 30.651 261 163 6 93 337 1 259 8.14e-30 116
Msa0509630 AT5G45370.5 30.651 261 163 6 93 337 1 259 8.14e-30 116
Msa0509630 AT1G60050.1 24.599 374 255 7 10 368 13 374 2.10e-29 117
Msa0509630 AT4G01450.4 32.701 211 134 2 30 239 30 233 2.28e-29 115
Msa0509630 AT3G28080.9 30.660 212 146 1 123 334 1 211 4.71e-28 110
Msa0509630 AT4G01450.3 34.043 188 116 2 30 216 30 210 6.18e-27 107
Msa0509630 AT3G45870.9 27.925 265 176 6 3 258 9 267 1.32e-26 107
Msa0509630 AT4G16620.1 28.904 301 202 5 35 330 37 330 1.67e-26 109
Msa0509630 AT3G28070.5 29.245 212 149 1 123 334 1 211 9.44e-26 104
Msa0509630 AT4G24980.1 26.059 307 158 4 24 330 1 238 2.23e-25 104
Msa0509630 AT3G45870.5 28.740 254 168 6 116 358 43 294 1.72e-24 102
Msa0509630 AT3G28070.3 26.537 309 218 4 4 308 11 314 3.00e-24 102
Msa0509630 AT3G28130.4 28.807 243 165 3 14 252 17 255 1.20e-23 99.4
Msa0509630 AT5G45370.3 30.180 222 140 5 124 332 74 293 7.90e-23 98.2
Msa0509630 AT3G28100.3 28.458 253 173 3 4 252 8 256 3.98e-22 95.1
Msa0509630 AT3G28080.3 26.269 335 184 6 4 334 8 283 5.00e-22 95.9
Msa0509630 AT1G43650.2 29.752 242 162 3 17 257 14 248 2.74e-21 92.8
Msa0509630 AT4G01430.3 26.957 230 157 4 138 358 1 228 8.41e-21 90.5
Msa0509630 AT3G28080.4 26.446 242 123 3 93 334 8 194 3.22e-20 88.6
Msa0509630 AT4G16620.4 29.583 240 158 4 95 330 1 233 5.33e-20 89.4
Msa0509630 AT4G16620.2 29.583 240 158 4 95 330 1 233 5.33e-20 89.4
Msa0509630 AT3G45870.2 34.028 144 93 2 182 325 42 183 1.67e-19 87.0
Msa0509630 AT3G45870.6 28.218 202 134 5 116 308 43 242 2.95e-18 84.0
Msa0509630 AT3G45870.7 28.218 202 134 5 116 308 43 242 2.95e-18 84.0
Msa0509630 AT3G28100.2 29.148 223 150 3 4 222 8 226 6.17e-17 80.1
Msa0509630 AT4G16620.3 30.964 197 130 5 40 234 41 233 3.79e-16 78.2
Msa0509630 AT3G28130.6 29.744 195 129 3 14 204 17 207 4.53e-15 74.3

Find 113 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATCGTTACTTTCATTTCT+AGG 0.166009 3_4:+58089117 None:intergenic
TCTAAATTTGTGAAAGTAAA+TGG 0.183752 3_4:-58088804 Msa0509630:three_prime_UTR
AGCCAAGACACATTTAATTT+TGG 0.208857 3_4:+58089043 None:intergenic
TGTCATTATCTCTTTGAAAA+TGG 0.218801 3_4:+58088992 None:intergenic
ATTTCGAGCTTTGTATTCTT+TGG 0.224631 3_4:+58089215 None:intergenic
CTATTGTTGGCTGTTCAATT+TGG 0.229434 3_4:-58091266 Msa0509630:CDS
TAGAATCTCAAACCACAAAA+TGG 0.244858 3_4:+58088823 None:intergenic
GCAACTTGGGTCTGTTTCAT+TGG 0.245357 3_4:-58089797 Msa0509630:CDS
AACTCTCATGCTTGGGCTCT+TGG 0.264844 3_4:-58089731 Msa0509630:CDS
ACCGGTGACCGGTGGATCAT+AGG 0.285377 3_4:-58090029 Msa0509630:CDS
TTGTTGGGAGTATTCATTAA+AGG 0.292892 3_4:-58088876 Msa0509630:three_prime_UTR
GGAGCAATAGTGGTGGTTCT+TGG 0.303174 3_4:-58089264 Msa0509630:CDS
TGTTACTAGAAAGAGGAATT+TGG 0.305169 3_4:-58088943 Msa0509630:three_prime_UTR
TGATCAATGCTTTGCCCTGC+TGG 0.312662 3_4:-58090775 Msa0509630:CDS
GAAAGAGGAATTTGGCCATT+TGG 0.314426 3_4:-58088935 Msa0509630:three_prime_UTR
ATCGGCACCATTGTAACATT+TGG 0.331344 3_4:-58090152 Msa0509630:CDS
CTAATTGCTCATTTAATTAG+TGG 0.336095 3_4:-58088903 Msa0509630:three_prime_UTR
CACCGATGTCTCTCGCAACT+TGG 0.358711 3_4:-58089811 Msa0509630:CDS
CTCATGCTTGGGCTCTTGGT+TGG 0.362607 3_4:-58089727 Msa0509630:CDS
GAGCAATAGTGGTGGTTCTT+GGG 0.363548 3_4:-58089263 Msa0509630:CDS
GCTGTTCAATTTGGTTCTGC+TGG 0.366885 3_4:-58091257 Msa0509630:CDS
TTAAAAGCAGTCACAATAAC+TGG 0.368606 3_4:+58089455 None:intergenic
TTGATTAAATAATATTGTTA+AGG 0.369440 3_4:-58088669 Msa0509630:three_prime_UTR
TCATGCTTGGGCTCTTGGTT+GGG 0.371511 3_4:-58089726 Msa0509630:CDS
CGATATTAATGAAGCTCAAT+TGG 0.371801 3_4:-58089100 Msa0509630:CDS
TTTAATTAGTGGTCACTTGT+TGG 0.384355 3_4:-58088892 Msa0509630:three_prime_UTR
ACTGGAACACATGAAGATTA+AGG 0.386490 3_4:-58090198 Msa0509630:CDS
AGCGCCACAACTCTCATGCT+TGG 0.393575 3_4:-58089739 Msa0509630:CDS
GGGACATGTTTCCTCCTCAT+TGG 0.394546 3_4:-58090008 Msa0509630:CDS
ACAGCCAACAATAGTAGATA+TGG 0.410774 3_4:+58091275 None:intergenic
GATCAATGCTTTGCCCTGCT+GGG 0.418619 3_4:-58090774 Msa0509630:CDS
TTAATTAGTGGTCACTTGTT+GGG 0.419073 3_4:-58088891 Msa0509630:three_prime_UTR
GAAACACTAACCGCGTAAGC+TGG 0.419739 3_4:+58089686 None:intergenic
TATGATCATCGTAACGGCCT+TGG 0.421783 3_4:-58089423 Msa0509630:CDS
ATTTGGAGGAACATTGCTAA+TGG 0.425413 3_4:-58090135 Msa0509630:CDS
TTCCTCCTCATTGGTTGCGC+TGG 0.426081 3_4:-58089999 Msa0509630:CDS
GGTAAACAATGAAGACATAA+TGG 0.433475 3_4:+58091192 None:intergenic
TGCAATGAGTCCTCCTTTGT+AGG 0.437328 3_4:+58089171 None:intergenic
TGGGCTTTATCTAGTTGTGT+GGG 0.443143 3_4:-58089244 Msa0509630:CDS
TAAAAGAGAGACTCAAAAGA+TGG 0.446644 3_4:-58091321 Msa0509630:exon
AAAACCATATCTACTATTGT+TGG 0.456596 3_4:-58091279 Msa0509630:CDS
CAGTATTATTGGAGCAATAG+TGG 0.462014 3_4:-58089274 Msa0509630:CDS
GCCATGGATGCTATCAAGAA+AGG 0.462957 3_4:-58091221 Msa0509630:CDS
AAACACTAACCGCGTAAGCT+GGG 0.471232 3_4:+58089687 None:intergenic
GCGCCACAACTCTCATGCTT+GGG 0.476115 3_4:-58089738 Msa0509630:CDS
CCGGTGACCGGTGGATCATA+GGG 0.478455 3_4:-58090028 Msa0509630:CDS
AGGAATTTGGCCATTTGGCA+TGG 0.479726 3_4:-58088930 Msa0509630:three_prime_UTR
AGAATCTCAAACCACAAAAT+GGG 0.487607 3_4:+58088824 None:intergenic
GTTGCGAGAGACATCGGTGC+AGG 0.489540 3_4:+58089815 None:intergenic
TTACAGCAGCGATGGCATTA+CGG 0.493658 3_4:+58091171 None:intergenic
GCCTTTCTTGATAGCATCCA+TGG 0.499872 3_4:+58091220 None:intergenic
AGGGTACTTTCTCAACGTTA+TGG 0.500304 3_4:+58089835 None:intergenic
TCACCGGTAGCTGCTGTTGT+TGG 0.503402 3_4:+58089145 None:intergenic
ATGATCCACCGGTCACCGGT+GGG 0.508383 3_4:+58090032 None:intergenic
GCAGTATTACATACAAGGTC+TGG 0.510999 3_4:-58089495 Msa0509630:CDS
ATCAATGCTTTGCCCTGCTG+GGG 0.512067 3_4:-58090773 Msa0509630:CDS
ACCGATGTCTCTCGCAACTT+GGG 0.512509 3_4:-58089810 Msa0509630:CDS
CCCTATGATCCACCGGTCAC+CGG 0.512612 3_4:+58090028 None:intergenic
GGCACCATTGTAACATTTGG+AGG 0.513427 3_4:-58090149 Msa0509630:CDS
TTGGGCTTTATCTAGTTGTG+TGG 0.514435 3_4:-58089245 Msa0509630:CDS
GGCCAAAATTAAATGTGTCT+TGG 0.520070 3_4:-58089045 Msa0509630:exon
ACGTTAACTTCATCCCCAGC+AGG 0.524214 3_4:+58090760 None:intergenic
TGAGTCCTCCTTTGTAGGTG+AGG 0.528022 3_4:+58089176 None:intergenic
TCCTCCTTTGTAGGTGAGGG+TGG 0.533108 3_4:+58089180 None:intergenic
ATGCATGTGTTACTAGAAAG+AGG 0.534032 3_4:-58088950 Msa0509630:three_prime_UTR
CCTTCGCATTTCATCTCGAA+AGG 0.535769 3_4:-58091139 Msa0509630:intron
AATTAGTGCTCCATGCCAAA+TGG 0.537015 3_4:+58088920 None:intergenic
GACCTCACAACACTAACTAC+AGG 0.541490 3_4:+58090098 None:intergenic
TATGATCCACCGGTCACCGG+TGG 0.544663 3_4:+58090031 None:intergenic
TGAGTGACCCCACCGGTGAC+CGG 0.547224 3_4:-58090040 Msa0509630:CDS
TGGCGCACTTCAAAGCTTCG+TGG 0.550157 3_4:-58089777 Msa0509630:CDS
CAGAAAAGTAACAGAGGGCG+CGG 0.556329 3_4:+58090707 None:intergenic
CTACAGGGGATAATTACATC+AGG 0.557819 3_4:-58089521 Msa0509630:intron
GGGCTTTATCTAGTTGTGTG+GGG 0.561431 3_4:-58089243 Msa0509630:CDS
GAGTCCTCCTTTGTAGGTGA+GGG 0.563130 3_4:+58089177 None:intergenic
TTGTATAAAGAGACATTGAT+CGG 0.568243 3_4:-58089020 Msa0509630:three_prime_UTR
AGCTAACGTTACAGCAGCGA+TGG 0.569797 3_4:+58091163 None:intergenic
GCAATGTGAAATTGCTAATG+TGG 0.571756 3_4:+58091360 None:intergenic
AAAAGAGAGACTCAAAAGAT+GGG 0.585764 3_4:-58091320 Msa0509630:exon
CGCAAACAGAAAAGTAACAG+AGG 0.588169 3_4:+58090701 None:intergenic
GTGACCCCACCGGTGACCGG+TGG 0.589517 3_4:-58090037 Msa0509630:CDS
TGATCCACCGGTCACCGGTG+GGG 0.592096 3_4:+58090033 None:intergenic
TGACCAACAACAGCAGCTAC+CGG 0.594681 3_4:-58089148 Msa0509630:CDS
TATGCCACCCTCACCTACAA+AGG 0.595290 3_4:-58089184 Msa0509630:CDS
ACTCTTTGCCCCAGCTTACG+CGG 0.595828 3_4:-58089696 Msa0509630:intron
AAAAGCCAGCGCAACCAATG+AGG 0.596960 3_4:+58089994 None:intergenic
GAGCCCAAGCATGAGAGTTG+TGG 0.598586 3_4:+58089735 None:intergenic
AACATGTCCCTATGATCCAC+CGG 0.601007 3_4:+58090021 None:intergenic
TCATTTCTAGGAACTGTCAC+CGG 0.604984 3_4:+58089129 None:intergenic
CCTTTCGAGATGAAATGCGA+AGG 0.605006 3_4:+58091139 None:intergenic
AACACTAACCGCGTAAGCTG+GGG 0.606336 3_4:+58089688 None:intergenic
AGCCAGCGCAACCAATGAGG+AGG 0.607748 3_4:+58089997 None:intergenic
ACCCAAGTTGCGAGAGACAT+CGG 0.608909 3_4:+58089809 None:intergenic
AATACACGGAGAGACATCTT+CGG 0.610930 3_4:+58090961 None:intergenic
GGAGTGCAGTATTACATACA+AGG 0.611210 3_4:-58089500 Msa0509630:CDS
AGTGTAAAAGATGTAATAGA+TGG 0.618839 3_4:+58088771 None:intergenic
TGTTCCTCCAAATGTTACAA+TGG 0.620453 3_4:+58090145 None:intergenic
GCAAACAGAAAAGTAACAGA+GGG 0.623135 3_4:+58090702 None:intergenic
TTGCTAATGGCTCTGTACAA+AGG 0.625645 3_4:-58090122 Msa0509630:CDS
CTTTCGAGATGAAATGCGAA+GGG 0.629918 3_4:+58091140 None:intergenic
TACCTGCAATATGTAAAATG+CGG 0.635765 3_4:+58089971 None:intergenic
AGAGAGGATGATGCAAGCCA+AGG 0.637732 3_4:+58089406 None:intergenic
CGTTAACTTCATCCCCAGCA+GGG 0.639969 3_4:+58090761 None:intergenic
CTGGCGGGTAGCATGACTGA+TGG 0.644522 3_4:+58090073 None:intergenic
TTGCGAGAGACATCGGTGCA+GGG 0.646982 3_4:+58089816 None:intergenic
GCCACCCTCACCTACAAAGG+AGG 0.652818 3_4:-58089181 Msa0509630:CDS
GTCCTGTAGTTAGTGTTGTG+AGG 0.655305 3_4:-58090100 Msa0509630:CDS
GTTACGATGATCATACGTAC+CGG 0.664727 3_4:+58089431 None:intergenic
AGACTCAAAAGATGGGTGCG+TGG 0.670730 3_4:-58091313 Msa0509630:exon
TATTATTGGAGCAATAGTGG+TGG 0.673331 3_4:-58089271 Msa0509630:CDS
ACATAATCTCTGAAAATACA+CGG 0.679552 3_4:+58090947 None:intergenic
GGTACGTATGATCATCGTAA+CGG 0.700079 3_4:-58089429 Msa0509630:CDS
GAAAATGTGAGTGACCCCAC+CGG 0.720215 3_4:-58090047 Msa0509630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATGATTAACATTTTAATT+AGG - chr3_4:58089005-58089024 Msa0509630:three_prime_UTR 10.0%
!!! ACATATTTTTATGTTTATTA+AGG - chr3_4:58090695-58090714 Msa0509630:CDS 10.0%
!! TTGATTAAATAATATTGTTA+AGG - chr3_4:58091364-58091383 Msa0509630:five_prime_UTR 10.0%
!!! TTTTTGCTTATATTTTGTTA+CGG - chr3_4:58088975-58088994 Msa0509630:three_prime_UTR 15.0%
!! TTAATCTTTATATCACTATA+TGG - chr3_4:58089132-58089151 Msa0509630:CDS 15.0%
!!! ATTTATAATGCAATACATTT+TGG - chr3_4:58089178-58089197 Msa0509630:CDS 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr3_4:58089497-58089516 Msa0509630:CDS 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr3_4:58089498-58089517 Msa0509630:CDS 15.0%
!! ACAAAAAAATAATGTCTATA+AGG - chr3_4:58089787-58089806 Msa0509630:CDS 15.0%
!! CAAAAAAATAATGTCTATAA+GGG - chr3_4:58089788-58089807 Msa0509630:CDS 15.0%
!!! TATTTGGTCTAAAATTTGAA+TGG + chr3_4:58088935-58088954 None:intergenic 20.0%
!! TCAAATAAAGTTAACGTATT+TGG + chr3_4:58088951-58088970 None:intergenic 20.0%
!!! AAATATGTGTTTCTTTGTTT+TGG - chr3_4:58089036-58089055 Msa0509630:three_prime_UTR 20.0%
!!! TAAGAAAAGTGTATAAATGA+AGG - chr3_4:58089432-58089451 Msa0509630:CDS 20.0%
!!! AAGAAAAGTGTATAAATGAA+GGG - chr3_4:58089433-58089452 Msa0509630:CDS 20.0%
!! AAAGTGGAATATATTGTATT+GGG - chr3_4:58089457-58089476 Msa0509630:CDS 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - chr3_4:58089567-58089586 Msa0509630:intron 20.0%
!!! CCTTTAAAACCTTTATATTA+CGG - chr3_4:58089719-58089738 Msa0509630:CDS 20.0%
!! GGGATGTTAATTAATTAAAT+TGG - chr3_4:58089808-58089827 Msa0509630:CDS 20.0%
!!! TTTTATGATGACAGTATTAT+TGG - chr3_4:58090748-58090767 Msa0509630:CDS 20.0%
!! TCTAAATTTGTGAAAGTAAA+TGG - chr3_4:58091229-58091248 Msa0509630:CDS 20.0%
! AAAACCATATCTACTATTGT+TGG - chr3_4:58088754-58088773 Msa0509630:three_prime_UTR 25.0%
!! TCTAAAATTTGAATGGAAGA+GGG + chr3_4:58088928-58088947 None:intergenic 25.0%
!!! AAGAAACACATATTTTAGCA+CGG + chr3_4:58089031-58089050 None:intergenic 25.0%
!! ACATAATCTCTGAAAATACA+CGG + chr3_4:58089089-58089108 None:intergenic 25.0%
!! ATTTTCAGAGATTATGTTAC+TGG - chr3_4:58089092-58089111 Msa0509630:CDS 25.0%
!! AGAAAAGTGTATAAATGAAG+GGG - chr3_4:58089434-58089453 Msa0509630:CDS 25.0%
! GAAAGTGGAATATATTGTAT+TGG - chr3_4:58089456-58089475 Msa0509630:CDS 25.0%
!! TTGAAATGAGTCAGTTATTT+TGG - chr3_4:58089544-58089563 Msa0509630:intron 25.0%
!!! TGAAATGAGTCAGTTATTTT+GGG - chr3_4:58089545-58089564 Msa0509630:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - chr3_4:58089566-58089585 Msa0509630:intron 25.0%
!!! CCGTAATATAAAGGTTTTAA+AGG + chr3_4:58089722-58089741 None:intergenic 25.0%
! TTAATGTAGCCGTAATATAA+AGG + chr3_4:58089731-58089750 None:intergenic 25.0%
! ATTAATTAAATTGGACAGAC+TGG - chr3_4:58089817-58089836 Msa0509630:CDS 25.0%
! GAAACATGTATGTATATGTT+TGG - chr3_4:58090153-58090172 Msa0509630:CDS 25.0%
! TAGTAATCAATCACAAGAAA+AGG + chr3_4:58090495-58090514 None:intergenic 25.0%
!! ATATCGTTACTTTCATTTCT+AGG + chr3_4:58090919-58090938 None:intergenic 25.0%
! TTGTATAAAGAGACATTGAT+CGG - chr3_4:58091013-58091032 Msa0509630:intron 25.0%
!! TGTCATTATCTCTTTGAAAA+TGG + chr3_4:58091044-58091063 None:intergenic 25.0%
! CTAATTGCTCATTTAATTAG+TGG - chr3_4:58091130-58091149 Msa0509630:intron 25.0%
! AGTGTAAAAGATGTAATAGA+TGG + chr3_4:58091265-58091284 None:intergenic 25.0%
TAAAAGAGAGACTCAAAAGA+TGG - chr3_4:58088712-58088731 Msa0509630:three_prime_UTR 30.0%
AAAAGAGAGACTCAAAAGAT+GGG - chr3_4:58088713-58088732 Msa0509630:three_prime_UTR 30.0%
GGTAAACAATGAAGACATAA+TGG + chr3_4:58088844-58088863 None:intergenic 30.0%
! GTCTAAAATTTGAATGGAAG+AGG + chr3_4:58088929-58088948 None:intergenic 30.0%
!!! TTTGTTTTGGCAGGAAAATT+AGG - chr3_4:58089049-58089068 Msa0509630:exon 30.0%
GTTAAGCGAATCATATATTG+CGG - chr3_4:58089640-58089659 Msa0509630:intron 30.0%
! ATTGTTAAGCTGACAGTTTT+TGG + chr3_4:58089754-58089773 None:intergenic 30.0%
GCATGTCAAGCAAAAATAAT+CGG - chr3_4:58089863-58089882 Msa0509630:intron 30.0%
TACCTGCAATATGTAAAATG+CGG + chr3_4:58090065-58090084 None:intergenic 30.0%
! GTTTAACGTGATGATTTTGT+AGG - chr3_4:58090175-58090194 Msa0509630:CDS 30.0%
TTCTTGTGATTGATTACTAC+AGG - chr3_4:58090496-58090515 Msa0509630:intron 30.0%
TCTTGTGATTGATTACTACA+GGG - chr3_4:58090497-58090516 Msa0509630:intron 30.0%
AGGTCTGGTTATAAAAACTA+TGG - chr3_4:58090553-58090572 Msa0509630:intron 30.0%
GGTCTGGTTATAAAAACTAT+GGG - chr3_4:58090554-58090573 Msa0509630:intron 30.0%
TTAAAAGCAGTCACAATAAC+TGG + chr3_4:58090581-58090600 None:intergenic 30.0%
!! TATTGTGACTGCTTTTAATC+CGG - chr3_4:58090583-58090602 Msa0509630:intron 30.0%
! ATTTCGAGCTTTGTATTCTT+TGG + chr3_4:58090821-58090840 None:intergenic 30.0%
CGATATTAATGAAGCTCAAT+TGG - chr3_4:58090933-58090952 Msa0509630:CDS 30.0%
!! AGCCAAGACACATTTAATTT+TGG + chr3_4:58090993-58091012 None:intergenic 30.0%
TGTTACTAGAAAGAGGAATT+TGG - chr3_4:58091090-58091109 Msa0509630:intron 30.0%
TTTAATTAGTGGTCACTTGT+TGG - chr3_4:58091141-58091160 Msa0509630:CDS 30.0%
TTAATTAGTGGTCACTTGTT+GGG - chr3_4:58091142-58091161 Msa0509630:CDS 30.0%
!! TTGTTGGGAGTATTCATTAA+AGG - chr3_4:58091157-58091176 Msa0509630:CDS 30.0%
AGAATCTCAAACCACAAAAT+GGG + chr3_4:58091212-58091231 None:intergenic 30.0%
TAGAATCTCAAACCACAAAA+TGG + chr3_4:58091213-58091232 None:intergenic 30.0%
GCAATGTGAAATTGCTAATG+TGG + chr3_4:58088676-58088695 None:intergenic 35.0%
! AGTCTCTCTTTTACTCTCTT+GGG + chr3_4:58088705-58088724 None:intergenic 35.0%
ACAGCCAACAATAGTAGATA+TGG + chr3_4:58088761-58088780 None:intergenic 35.0%
! CTATTGTTGGCTGTTCAATT+TGG - chr3_4:58088767-58088786 Msa0509630:three_prime_UTR 35.0%
!! TGGTATGTTCATTTTTGCCA+TGG - chr3_4:58088796-58088815 Msa0509630:three_prime_UTR 35.0%
!!! ATGTGTTTCTTTGTTTTGGC+AGG - chr3_4:58089040-58089059 Msa0509630:exon 35.0%
GCAAACAGAAAAGTAACAGA+GGG + chr3_4:58089334-58089353 None:intergenic 35.0%
!!! CTCTGTTACTTTTCTGTTTG+CGG - chr3_4:58089333-58089352 Msa0509630:intron 35.0%
CAAAGTAGGTCAATTATACC+GGG + chr3_4:58089397-58089416 None:intergenic 35.0%
CCAAAGTAGGTCAATTATAC+CGG + chr3_4:58089398-58089417 None:intergenic 35.0%
TCTGTGTGAAAAACCAAAGT+AGG + chr3_4:58089411-58089430 None:intergenic 35.0%
TGTATAAATGAAGGGGAAAG+TGG - chr3_4:58089441-58089460 Msa0509630:CDS 35.0%
AGAAAATGGGTCAGTTATTC+CGG - chr3_4:58089580-58089599 Msa0509630:intron 35.0%
CGGTAAAATGCAAACAAATG+CGG - chr3_4:58089660-58089679 Msa0509630:intron 35.0%
ACTGGAACACATGAAGATTA+AGG - chr3_4:58089835-58089854 Msa0509630:CDS 35.0%
TGTTCCTCCAAATGTTACAA+TGG + chr3_4:58089891-58089910 None:intergenic 35.0%
ATTTGGAGGAACATTGCTAA+TGG - chr3_4:58089898-58089917 Msa0509630:intron 35.0%
! TTCCGCATTTTACATATTGC+AGG - chr3_4:58090060-58090079 Msa0509630:CDS 35.0%
AACTCGAAAGGTGAATTCTA+TGG + chr3_4:58090391-58090410 None:intergenic 35.0%
CTTGTGATTGATTACTACAG+GGG - chr3_4:58090498-58090517 Msa0509630:intron 35.0%
CAAGAAAAAGTTGTTCAGAG+AGG + chr3_4:58090646-58090665 None:intergenic 35.0%
!! CTCTCTGAACAACTTTTTCT+TGG - chr3_4:58090644-58090663 Msa0509630:intron 35.0%
!! CTGAACAACTTTTTCTTGGA+AGG - chr3_4:58090648-58090667 Msa0509630:intron 35.0%
CAGTATTATTGGAGCAATAG+TGG - chr3_4:58090759-58090778 Msa0509630:CDS 35.0%
TATTATTGGAGCAATAGTGG+TGG - chr3_4:58090762-58090781 Msa0509630:CDS 35.0%
CACAGTGAAGAAAAATGTCA+CGG - chr3_4:58090961-58090980 Msa0509630:CDS 35.0%
GGCCAAAATTAAATGTGTCT+TGG - chr3_4:58090988-58091007 Msa0509630:intron 35.0%
! ATGCATGTGTTACTAGAAAG+AGG - chr3_4:58091083-58091102 Msa0509630:intron 35.0%
!! AAAAAGTGTGACCCATTTTG+TGG - chr3_4:58091198-58091217 Msa0509630:CDS 35.0%
! GAGTCTCTCTTTTACTCTCT+TGG + chr3_4:58088706-58088725 None:intergenic 40.0%
CTTTCGAGATGAAATGCGAA+GGG + chr3_4:58088896-58088915 None:intergenic 40.0%
AATACACGGAGAGACATCTT+CGG + chr3_4:58089075-58089094 None:intergenic 40.0%
CGCAAACAGAAAAGTAACAG+AGG + chr3_4:58089335-58089354 None:intergenic 40.0%
CCGGTATAATTGACCTACTT+TGG - chr3_4:58089395-58089414 Msa0509630:CDS 40.0%
GAAAATGGGTCAGTTATTCC+GGG - chr3_4:58089581-58089600 Msa0509630:intron 40.0%
ATCGGCACCATTGTAACATT+TGG - chr3_4:58089881-58089900 Msa0509630:intron 40.0%
! TTGCTAATGGCTCTGTACAA+AGG - chr3_4:58089911-58089930 Msa0509630:intron 40.0%
! AGGGTACTTTCTCAACGTTA+TGG + chr3_4:58090201-58090220 None:intergenic 40.0%
GTGCGTATTCTAAACTCGAA+AGG + chr3_4:58090403-58090422 None:intergenic 40.0%
CTACAGGGGATAATTACATC+AGG - chr3_4:58090512-58090531 Msa0509630:intron 40.0%
GGAGTGCAGTATTACATACA+AGG - chr3_4:58090533-58090552 Msa0509630:intron 40.0%
GCAGTATTACATACAAGGTC+TGG - chr3_4:58090538-58090557 Msa0509630:intron 40.0%
GTTACGATGATCATACGTAC+CGG + chr3_4:58090605-58090624 None:intergenic 40.0%
GGTACGTATGATCATCGTAA+CGG - chr3_4:58090604-58090623 Msa0509630:intron 40.0%
! TTGGGCTTTATCTAGTTGTG+TGG - chr3_4:58090788-58090807 Msa0509630:intron 40.0%
! TGGGCTTTATCTAGTTGTGT+GGG - chr3_4:58090789-58090808 Msa0509630:intron 40.0%
! TCATTTCTAGGAACTGTCAC+CGG + chr3_4:58090907-58090926 None:intergenic 40.0%
GAAGAAAAATGTCACGGTTG+TGG - chr3_4:58090967-58090986 Msa0509630:CDS 40.0%
GAAAGAGGAATTTGGCCATT+TGG - chr3_4:58091098-58091117 Msa0509630:intron 40.0%
AATTAGTGCTCCATGCCAAA+TGG + chr3_4:58091116-58091135 None:intergenic 40.0%
!! GCTGTTCAATTTGGTTCTGC+TGG - chr3_4:58088776-58088795 Msa0509630:three_prime_UTR 45.0%
GCCATGGATGCTATCAAGAA+AGG - chr3_4:58088812-58088831 Msa0509630:three_prime_UTR 45.0%
! GCCTTTCTTGATAGCATCCA+TGG + chr3_4:58088816-58088835 None:intergenic 45.0%
TTACAGCAGCGATGGCATTA+CGG + chr3_4:58088865-58088884 None:intergenic 45.0%
CCTTTCGAGATGAAATGCGA+AGG + chr3_4:58088897-58088916 None:intergenic 45.0%
CCTTCGCATTTCATCTCGAA+AGG - chr3_4:58088894-58088913 Msa0509630:three_prime_UTR 45.0%
GTAGGTCAATTATACCGGGA+CGG + chr3_4:58089393-58089412 None:intergenic 45.0%
GGCACCATTGTAACATTTGG+AGG - chr3_4:58089884-58089903 Msa0509630:intron 45.0%
GTCCTGTAGTTAGTGTTGTG+AGG - chr3_4:58089933-58089952 Msa0509630:intron 45.0%
GACCTCACAACACTAACTAC+AGG + chr3_4:58089938-58089957 None:intergenic 45.0%
AACATGTCCCTATGATCCAC+CGG + chr3_4:58090015-58090034 None:intergenic 45.0%
GCAACTTGGGTCTGTTTCAT+TGG - chr3_4:58090236-58090255 Msa0509630:intron 45.0%
AAACACTAACCGCGTAAGCT+GGG + chr3_4:58090349-58090368 None:intergenic 45.0%
TTGTGTCCAAATAGCGCAAG+TGG + chr3_4:58090429-58090448 None:intergenic 45.0%
TATGATCATCGTAACGGCCT+TGG - chr3_4:58090610-58090629 Msa0509630:intron 45.0%
GAGCAATAGTGGTGGTTCTT+GGG - chr3_4:58090770-58090789 Msa0509630:CDS 45.0%
! GGGCTTTATCTAGTTGTGTG+GGG - chr3_4:58090790-58090809 Msa0509630:intron 45.0%
TGCAATGAGTCCTCCTTTGT+AGG + chr3_4:58090865-58090884 None:intergenic 45.0%
AGGAATTTGGCCATTTGGCA+TGG - chr3_4:58091103-58091122 Msa0509630:intron 45.0%
AGACTCAAAAGATGGGTGCG+TGG - chr3_4:58088720-58088739 Msa0509630:three_prime_UTR 50.0%
AGCTAACGTTACAGCAGCGA+TGG + chr3_4:58088873-58088892 None:intergenic 50.0%
TGATCAATGCTTTGCCCTGC+TGG - chr3_4:58089258-58089277 Msa0509630:CDS 50.0%
GATCAATGCTTTGCCCTGCT+GGG - chr3_4:58089259-58089278 Msa0509630:CDS 50.0%
ATCAATGCTTTGCCCTGCTG+GGG - chr3_4:58089260-58089279 Msa0509630:CDS 50.0%
CGTTAACTTCATCCCCAGCA+GGG + chr3_4:58089275-58089294 None:intergenic 50.0%
ACGTTAACTTCATCCCCAGC+AGG + chr3_4:58089276-58089295 None:intergenic 50.0%
CAGAAAAGTAACAGAGGGCG+CGG + chr3_4:58089329-58089348 None:intergenic 50.0%
AAATACTACTCCCTCCGTCC+CGG - chr3_4:58089376-58089395 Msa0509630:intron 50.0%
GTCAATTATACCGGGACGGA+GGG + chr3_4:58089389-58089408 None:intergenic 50.0%
ATGGGTCAGTTATTCCGGGA+CGG - chr3_4:58089585-58089604 Msa0509630:intron 50.0%
!! ACTCACATTTTCAGGCTGGC+GGG + chr3_4:58089978-58089997 None:intergenic 50.0%
!! CACTCACATTTTCAGGCTGG+CGG + chr3_4:58089979-58089998 None:intergenic 50.0%
!! GGTCACTCACATTTTCAGGC+TGG + chr3_4:58089982-58090001 None:intergenic 50.0%
! GTGGGGTCACTCACATTTTC+AGG + chr3_4:58089986-58090005 None:intergenic 50.0%
GAAAATGTGAGTGACCCCAC+CGG - chr3_4:58089986-58090005 Msa0509630:CDS 50.0%
GGGACATGTTTCCTCCTCAT+TGG - chr3_4:58090025-58090044 Msa0509630:CDS 50.0%
AAAAGCCAGCGCAACCAATG+AGG + chr3_4:58090042-58090061 None:intergenic 50.0%
ACCGATGTCTCTCGCAACTT+GGG - chr3_4:58090223-58090242 Msa0509630:intron 50.0%
ACCCAAGTTGCGAGAGACAT+CGG + chr3_4:58090227-58090246 None:intergenic 50.0%
AACTCTCATGCTTGGGCTCT+TGG - chr3_4:58090302-58090321 Msa0509630:intron 50.0%
TCATGCTTGGGCTCTTGGTT+GGG - chr3_4:58090307-58090326 Msa0509630:intron 50.0%
AACACTAACCGCGTAAGCTG+GGG + chr3_4:58090348-58090367 None:intergenic 50.0%
GAAACACTAACCGCGTAAGC+TGG + chr3_4:58090350-58090369 None:intergenic 50.0%
CACCAACCACTTGCGCTATT+TGG - chr3_4:58090420-58090439 Msa0509630:intron 50.0%
GTCCAAATAGCGCAAGTGGT+TGG + chr3_4:58090425-58090444 None:intergenic 50.0%
AGAGAGGATGATGCAAGCCA+AGG + chr3_4:58090630-58090649 None:intergenic 50.0%
GGAGCAATAGTGGTGGTTCT+TGG - chr3_4:58090769-58090788 Msa0509630:CDS 50.0%
TATGCCACCCTCACCTACAA+AGG - chr3_4:58090849-58090868 Msa0509630:intron 50.0%
GAGTCCTCCTTTGTAGGTGA+GGG + chr3_4:58090859-58090878 None:intergenic 50.0%
TGAGTCCTCCTTTGTAGGTG+AGG + chr3_4:58090860-58090879 None:intergenic 50.0%
TGACCAACAACAGCAGCTAC+CGG - chr3_4:58090885-58090904 Msa0509630:intron 50.0%
GGTCAATTATACCGGGACGG+AGG + chr3_4:58089390-58089409 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG - chr3_4:58089589-58089608 Msa0509630:intron 55.0%
ATCAGATACTCCCTCCGTCC+CGG + chr3_4:58089602-58089621 None:intergenic 55.0%
! TTCCTCCTCATTGGTTGCGC+TGG - chr3_4:58090034-58090053 Msa0509630:CDS 55.0%
TTGCGAGAGACATCGGTGCA+GGG + chr3_4:58090220-58090239 None:intergenic 55.0%
CACCGATGTCTCTCGCAACT+TGG - chr3_4:58090222-58090241 Msa0509630:intron 55.0%
TGGCGCACTTCAAAGCTTCG+TGG - chr3_4:58090256-58090275 Msa0509630:intron 55.0%
AGCGCCACAACTCTCATGCT+TGG - chr3_4:58090294-58090313 Msa0509630:intron 55.0%
GCGCCACAACTCTCATGCTT+GGG - chr3_4:58090295-58090314 Msa0509630:intron 55.0%
GAGCCCAAGCATGAGAGTTG+TGG + chr3_4:58090301-58090320 None:intergenic 55.0%
CTCATGCTTGGGCTCTTGGT+TGG - chr3_4:58090306-58090325 Msa0509630:intron 55.0%
ACTCTTTGCCCCAGCTTACG+CGG - chr3_4:58090337-58090356 Msa0509630:intron 55.0%
TCCTCCTTTGTAGGTGAGGG+TGG + chr3_4:58090856-58090875 None:intergenic 55.0%
TCACCGGTAGCTGCTGTTGT+TGG + chr3_4:58090891-58090910 None:intergenic 55.0%
GGTCAGTTATTCCGGGACGG+AGG - chr3_4:58089588-58089607 Msa0509630:intron 60.0%
! CTGGCGGGTAGCATGACTGA+TGG + chr3_4:58089963-58089982 None:intergenic 60.0%
ATGATCCACCGGTCACCGGT+GGG + chr3_4:58090004-58090023 None:intergenic 60.0%
TATGATCCACCGGTCACCGG+TGG + chr3_4:58090005-58090024 None:intergenic 60.0%
ACCGGTGACCGGTGGATCAT+AGG - chr3_4:58090004-58090023 Msa0509630:CDS 60.0%
CCCTATGATCCACCGGTCAC+CGG + chr3_4:58090008-58090027 None:intergenic 60.0%
CCGGTGACCGGTGGATCATA+GGG - chr3_4:58090005-58090024 Msa0509630:CDS 60.0%
AGCCAGCGCAACCAATGAGG+AGG + chr3_4:58090039-58090058 None:intergenic 60.0%
GTTGCGAGAGACATCGGTGC+AGG + chr3_4:58090221-58090240 None:intergenic 60.0%
GCCACCCTCACCTACAAAGG+AGG - chr3_4:58090852-58090871 Msa0509630:intron 60.0%
TGAGTGACCCCACCGGTGAC+CGG - chr3_4:58089993-58090012 Msa0509630:CDS 65.0%
TGATCCACCGGTCACCGGTG+GGG + chr3_4:58090003-58090022 None:intergenic 65.0%
GTGACCCCACCGGTGACCGG+TGG - chr3_4:58089996-58090015 Msa0509630:CDS 75.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 58088664 58091391 58088664 ID=Msa0509630;Name=Msa0509630
chr3_4 mRNA 58088664 58091391 58088664 ID=Msa0509630-mRNA-1;Parent=Msa0509630;Name=Msa0509630-mRNA-1;_AED=0.30;_eAED=0.30;_QI=67|1|1|1|1|1|7|392|387
chr3_4 exon 58088664 58089293 58088664 ID=Msa0509630-mRNA-1:exon:13476;Parent=Msa0509630-mRNA-1
chr3_4 exon 58089386 58089537 58089386 ID=Msa0509630-mRNA-1:exon:13475;Parent=Msa0509630-mRNA-1
chr3_4 exon 58089697 58089858 58089697 ID=Msa0509630-mRNA-1:exon:13474;Parent=Msa0509630-mRNA-1
chr3_4 exon 58089974 58090220 58089974 ID=Msa0509630-mRNA-1:exon:13473;Parent=Msa0509630-mRNA-1
chr3_4 exon 58090690 58090806 58090690 ID=Msa0509630-mRNA-1:exon:13472;Parent=Msa0509630-mRNA-1
chr3_4 exon 58090931 58090993 58090931 ID=Msa0509630-mRNA-1:exon:13471;Parent=Msa0509630-mRNA-1
chr3_4 exon 58091140 58091391 58091140 ID=Msa0509630-mRNA-1:exon:13470;Parent=Msa0509630-mRNA-1
chr3_4 five_prime_UTR 58091325 58091391 58091325 ID=Msa0509630-mRNA-1:five_prime_utr;Parent=Msa0509630-mRNA-1
chr3_4 CDS 58091140 58091324 58091140 ID=Msa0509630-mRNA-1:cds;Parent=Msa0509630-mRNA-1
chr3_4 CDS 58090931 58090993 58090931 ID=Msa0509630-mRNA-1:cds;Parent=Msa0509630-mRNA-1
chr3_4 CDS 58090690 58090806 58090690 ID=Msa0509630-mRNA-1:cds;Parent=Msa0509630-mRNA-1
chr3_4 CDS 58089974 58090220 58089974 ID=Msa0509630-mRNA-1:cds;Parent=Msa0509630-mRNA-1
chr3_4 CDS 58089697 58089858 58089697 ID=Msa0509630-mRNA-1:cds;Parent=Msa0509630-mRNA-1
chr3_4 CDS 58089386 58089537 58089386 ID=Msa0509630-mRNA-1:cds;Parent=Msa0509630-mRNA-1
chr3_4 CDS 58089056 58089293 58089056 ID=Msa0509630-mRNA-1:cds;Parent=Msa0509630-mRNA-1
chr3_4 three_prime_UTR 58088664 58089055 58088664 ID=Msa0509630-mRNA-1:three_prime_utr;Parent=Msa0509630-mRNA-1
Gene Sequence

>Msa0509630

ATGGGTGCGTGGTTGAAAAATGCAAAACCATATCTACTATTGTTGGCTGTTCAATTTGGTTCTGCTGGTATGTTCATTTTTGCCATGGATGCTATCAAGAAAGGCATGAGCCATTATGTCTTCATTGTTTACCGTAATGCCATCGCTGCTGTAACGTTAGCTCCCTTCGCATTTCATCTCGAAAGGAAAATTAGGCCGAAGATGTCTCTCCGTGTATTTTCAGAGATTATGTTACTGGCTTTCTTTGAAATAATACTTGATCAATGCTTTGCCCTGCTGGGGATGAAGTTAACGTCTGCGTCATTCTTGTCAGCTGTGATGAACTCCGCGCCCTCTGTTACTTTTCTGTTTGCGGTCATTCTAAAACTGGAACACATGAAGATTAAGGAGATAGCATGTCAAGCAAAAATAATCGGCACCATTGTAACATTTGGAGGAACATTGCTAATGGCTCTGTACAAAGGTCCTGTAGTTAGTGTTGTGAGGTCATCCATCAGTCATGCTACCCGCCAGCCTGAAAATGTGAGTGACCCCACCGGTGACCGGTGGATCATAGGGACATGTTTCCTCCTCATTGGTTGCGCTGGCTTTTCCGCATTTTACATATTGCAGGCCATAACGTTGAGAAAGTACCCTGCACCGATGTCTCTCGCAACTTGGGTCTGTTTCATTGGCGCACTTCAAAGCTTCGTGGTAGCATTCTTCGCAGAGCGCCACAACTCTCATGCTTGGGCTCTTGGTTGGGATACAAGACTCTTTGCCCCAGCTTACGCGGGGATAATTACATCAGGAGTGCAGTATTACATACAAGGTCTGGTTATAAAAACTATGGGACCAGTTATTGTGACTGCTTTTAATCCGGTACGTATGATCATCGTAACGGCCTTGGCTTGCATCATCCTCTCTGAACAACTTTTTCTTGGAAGTATTATTGGAGCAATAGTGGTGGTTCTTGGGCTTTATCTAGTTGTGTGGGGAAAATCCAAAGAATACAAAGCTCGAAATCATATTCATATGCCACCCTCACCTACAAAGGAGGACTCATTGCATGACCAACAACAGCAGCTACCGGTGACAGTTCCTAGAAATGAAAGTAACGATATTAATGAAGCTCAATTGGTCATTCACAGTGAAGAAAAATGTCACGGTTGTGGCCAAAATTAA
Protein sequence

>Msa0509630

MGAWLKNAKPYLLLLAVQFGSAGMFIFAMDAIKKGMSHYVFIVYRNAIAAVTLAPFAFHLERKIRPKMSLRVFSEIMLLAFFEIILDQCFALLGMKLTSASFLSAVMNSAPSVTFLFAVILKLEHMKIKEIACQAKIIGTIVTFGGTLLMALYKGPVVSVVRSSISHATRQPENVSDPTGDRWIIGTCFLLIGCAGFSAFYILQAITLRKYPAPMSLATWVCFIGALQSFVVAFFAERHNSHAWALGWDTRLFAPAYAGIITSGVQYYIQGLVIKTMGPVIVTAFNPVRMIIVTALACIILSEQLFLGSIIGAIVVVLGLYLVVWGKSKEYKARNHIHMPPSPTKEDSLHDQQQQLPVTVPRNESNDINEAQLVIHSEEKCHGCGQN*