AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0459400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459400 MtrunA17_Chr3g0107591 90.397 302 26 1 1 299 1 302 0.0 562
Msa0459400 MtrunA17_Chr4g0008111 80.212 283 56 0 14 296 18 300 1.29e-168 471
Msa0459400 MtrunA17_Chr8g0372671 71.096 301 83 1 1 297 1 301 4.39e-158 445
Msa0459400 MtrunA17_Chr8g0372821 68.647 303 91 1 1 299 1 303 6.03e-155 437
Msa0459400 MtrunA17_Chr5g0404631 67.466 292 94 1 9 299 23 314 7.37e-152 430
Msa0459400 MtrunA17_Chr6g0475221 69.366 284 79 2 14 297 18 293 4.73e-140 399
Msa0459400 MtrunA17_Chr6g0475241 68.070 285 88 1 14 298 19 300 1.78e-139 397
Msa0459400 MtrunA17_Chr8g0343971 63.448 290 105 1 10 298 14 303 8.68e-134 383
Msa0459400 MtrunA17_Chr8g0343981 65.217 276 95 1 10 284 14 289 3.30e-131 377
Msa0459400 MtrunA17_Chr3g0144871 44.863 292 147 3 10 291 14 301 7.77e-87 264
Msa0459400 MtrunA17_Chr3g0107871 42.336 274 148 3 21 288 2 271 3.76e-73 228
Msa0459400 MtrunA17_Chr5g0419851 39.322 295 169 2 1 285 61 355 2.39e-72 229
Msa0459400 MtrunA17_Chr8g0372801 77.692 130 29 0 86 215 31 160 1.16e-71 219
Msa0459400 MtrunA17_Chr7g0252361 39.194 273 155 3 22 288 24 291 4.42e-64 206
Msa0459400 MtrunA17_Chr1g0208641 37.234 282 147 8 19 284 31 298 1.93e-44 155
Msa0459400 MtrunA17_Chr5g0444921 36.641 262 150 5 47 293 70 330 1.43e-43 153
Msa0459400 MtrunA17_Chr3g0100101 36.626 243 143 3 50 281 63 305 2.78e-42 149
Msa0459400 MtrunA17_Chr1g0155451 44.203 138 71 1 157 288 9 146 9.16e-40 138
Msa0459400 MtrunA17_Chr6g0455071 51.327 113 45 2 133 239 11 119 8.37e-34 120
Msa0459400 MtrunA17_Chr1g0155461 44.444 135 65 5 21 145 25 159 6.18e-29 109
Msa0459400 MtrunA17_Chr1g0155491 42.466 146 74 4 10 145 14 159 1.40e-28 108
Msa0459400 MtrunA17_Chr7g0252501 38.400 125 71 1 164 288 38 156 1.91e-25 101
Msa0459400 MtrunA17_Chr7g0252341 46.364 110 53 3 50 157 27 132 3.11e-24 95.9
Msa0459400 MtrunA17_Chr1g0155481 48.193 83 43 0 159 241 17 99 1.28e-22 91.7
Msa0459400 MtrunA17_Chr3g0099281 38.596 114 67 1 47 157 68 181 2.54e-19 84.0
Msa0459400 MtrunA17_Chr3g0099271 36.765 136 79 3 164 293 15 149 7.01e-16 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459400 AT3G11480.1 46.014 276 138 4 10 280 36 305 1.06e-81 251
Msa0459400 AT5G66430.1 44.891 274 144 3 10 281 6 274 4.64e-81 249
Msa0459400 AT5G04380.3 46.296 270 132 4 21 285 54 315 1.13e-79 247
Msa0459400 AT3G21950.1 46.520 273 142 3 10 281 11 280 3.75e-77 239
Msa0459400 AT5G04370.1 44.891 274 145 3 10 281 46 315 6.65e-77 240
Msa0459400 AT5G04380.2 47.410 251 123 3 36 285 2 244 7.15e-77 237
Msa0459400 AT5G38020.1 46.886 273 141 3 10 281 11 280 1.68e-76 238
Msa0459400 AT5G04370.3 44.891 274 145 3 10 281 51 320 1.83e-76 239
Msa0459400 AT5G04370.2 44.891 274 145 3 10 281 65 334 2.07e-76 239
Msa0459400 AT5G04380.1 45.556 270 127 5 21 285 50 304 4.98e-75 234
Msa0459400 AT1G19640.1 44.194 310 151 5 1 288 1 310 2.09e-74 233
Msa0459400 AT4G36470.1 43.289 298 144 6 7 285 10 301 2.64e-74 233
Msa0459400 AT5G38020.2 50.000 238 116 2 44 281 7 241 4.79e-73 228
Msa0459400 AT3G21950.2 41.447 304 142 4 10 280 11 311 1.72e-70 223
Msa0459400 AT2G14060.1 42.647 272 152 2 10 280 19 287 1.87e-70 223
Msa0459400 AT2G14060.2 43.182 220 121 2 10 228 19 235 5.85e-59 189
Msa0459400 AT1G68040.1 36.268 284 156 6 10 281 18 288 1.26e-47 163
Msa0459400 AT5G55250.2 35.741 263 157 5 31 281 9 271 1.22e-46 160
Msa0459400 AT5G55250.1 34.507 284 174 5 10 281 26 309 2.12e-46 160
Msa0459400 AT5G38100.3 40.400 250 123 10 46 282 31 267 7.93e-43 150
Msa0459400 AT5G38100.1 40.400 250 123 10 46 282 54 290 1.37e-42 150
Msa0459400 AT1G15125.1 34.028 288 157 10 10 281 6 276 1.26e-40 144
Msa0459400 AT4G26420.1 34.559 272 154 8 25 280 33 296 1.76e-40 145
Msa0459400 AT5G37970.1 37.143 245 130 5 48 280 59 291 2.89e-38 139
Msa0459400 AT5G38780.2 34.701 268 138 9 47 295 7 256 1.57e-37 135
Msa0459400 AT5G56300.1 29.682 283 174 6 14 280 33 306 1.87e-37 137
Msa0459400 AT5G38780.1 34.701 268 138 9 47 295 56 305 3.33e-37 136
Msa0459400 AT3G44870.1 32.558 301 150 8 4 280 3 274 8.24e-37 135
Msa0459400 AT3G44840.1 33.808 281 163 7 10 281 12 278 1.41e-36 134
Msa0459400 AT3G44860.1 33.688 282 138 7 21 280 23 277 2.68e-36 133
Msa0459400 AT5G37990.1 36.179 246 131 6 48 280 59 291 1.02e-35 132
Msa0459400 AT1G66720.1 31.849 292 176 8 10 290 14 293 1.09e-35 132
Msa0459400 AT1G66700.1 29.930 284 171 7 10 280 14 282 3.94e-33 125
Msa0459400 AT5G38100.4 42.197 173 81 6 46 209 54 216 6.79e-31 116
Msa0459400 AT4G26420.3 35.500 200 111 7 25 214 33 224 7.33e-31 116
Msa0459400 AT5G38100.2 42.197 173 81 6 46 209 54 216 7.35e-31 116
Msa0459400 AT4G26420.2 35.500 200 111 7 25 214 21 212 1.19e-30 116
Msa0459400 AT1G66690.1 29.252 294 160 8 10 280 14 282 1.06e-29 115
Msa0459400 AT1G15125.2 34.419 215 116 8 10 213 6 206 1.23e-27 108
Msa0459400 AT1G66700.3 31.905 210 120 5 10 209 14 210 2.90e-25 102

Find 88 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGACTTGGGTTGCTCTTT+TGG 0.137193 3_3:-55903230 Msa0459400:CDS
TATAACCAGTCTATATTATA+TGG 0.186746 3_3:-55903375 Msa0459400:CDS
TGGAGGTTTCTGAAGTAAAT+TGG 0.236226 3_3:-55902390 Msa0459400:CDS
CCTCTAAGACCATATCATTA+AGG 0.240938 3_3:+55902594 None:intergenic
CGGGGAATGTGTTGCAATAT+AGG 0.287603 3_3:+55903267 None:intergenic
GCAAGGAGTGTTGTTACATT+TGG 0.289796 3_3:-55902634 Msa0459400:CDS
GTCTGCAATTGCCAAGCTTC+CGG 0.298575 3_3:+55903247 None:intergenic
GAGCACCTTCAACTAATTCT+TGG 0.304324 3_3:+55902702 None:intergenic
TAACAACACTCCTTGCTTGA+TGG 0.305696 3_3:+55902641 None:intergenic
TGTTACATCTATGGGGTTCC+TGG 0.309701 3_3:-55903014 Msa0459400:CDS
ATCACATAGTTTCTCTTTGA+AGG 0.315688 3_3:+55903061 None:intergenic
ACAATATGGCACAATGCATT+AGG 0.317665 3_3:-55902297 Msa0459400:three_prime_UTR
GATGGAGATGGATAATAGTT+AGG 0.322863 3_3:+55902463 None:intergenic
ATAGGCCCGTGTTACATCTA+TGG 0.324301 3_3:-55903023 Msa0459400:CDS
GGTGAACTTAGTCTTCTCTT+TGG 0.329569 3_3:+55902180 None:intergenic
ATCATTTGTCATCAATAAAC+TGG 0.330242 3_3:-55902410 Msa0459400:CDS
CCTTAATGATATGGTCTTAG+AGG 0.336456 3_3:-55902594 Msa0459400:intron
CAAGGAGTGTTGTTACATTT+GGG 0.342418 3_3:-55902633 Msa0459400:CDS
CAGTCTTCAATGGCTATCTA+AGG 0.342755 3_3:-55902934 Msa0459400:intron
GGCTATCTCCCTTAATGATA+TGG 0.343052 3_3:-55902603 Msa0459400:CDS
ACAAAATGCAAACTATTATC+TGG 0.350999 3_3:+55902969 None:intergenic
CTTTGGTGAAGGTGTCATTA+AGG 0.358741 3_3:-55902245 Msa0459400:three_prime_UTR
ATAACTAACAACATATATAT+TGG 0.362994 3_3:+55901990 None:intergenic
TCTGCAATTGCCAAGCTTCC+GGG 0.370317 3_3:+55903248 None:intergenic
AGAGAAACTATGTGATGAAA+TGG 0.370752 3_3:-55903054 Msa0459400:CDS
AGCACCTTCAACTAATTCTT+GGG 0.387792 3_3:+55902703 None:intergenic
ATTGGAATGCTCTTGATGAT+TGG 0.399506 3_3:-55902372 Msa0459400:CDS
TTGTTGTTGTCAGCACCCTC+TGG 0.403474 3_3:+55902824 None:intergenic
AAGCTTGGCAATTGCAGACT+TGG 0.404644 3_3:-55903243 Msa0459400:CDS
GATATCACCAACAAAGTGTT+TGG 0.405615 3_3:+55903206 None:intergenic
GATGAAATGGAAACTGAAAT+AGG 0.407347 3_3:-55903041 Msa0459400:CDS
GTTGCAATATAGGTTGGTTA+TGG 0.409482 3_3:+55903277 None:intergenic
TAGAGATGATGGATACAATA+TGG 0.413499 3_3:-55902311 Msa0459400:three_prime_UTR
TGTCAACGATATGCTAAGAT+TGG 0.419291 3_3:+55902159 None:intergenic
CAATATGGCACAATGCATTA+GGG 0.420742 3_3:-55902296 Msa0459400:three_prime_UTR
CTCTAAGACCATATCATTAA+GGG 0.438228 3_3:+55902595 None:intergenic
TCAGAATCACTTAGAGATGA+TGG 0.456810 3_3:-55902322 Msa0459400:three_prime_UTR
ATCTCCATCTGAAGTGAAGT+TGG 0.456867 3_3:-55902449 Msa0459400:CDS
TAGGCCCGTGTTACATCTAT+GGG 0.457629 3_3:-55903022 Msa0459400:CDS
ACGTTGTTGAAGTCATTTCC+CGG 0.462185 3_3:+55903101 None:intergenic
CTAGTTAGTCACTTTGGTGA+AGG 0.467251 3_3:-55902256 Msa0459400:three_prime_UTR
CCTTTGCTAGTTAGTCACTT+TGG 0.468922 3_3:-55902262 Msa0459400:three_prime_UTR
CAACACATTCCCCGGAAGCT+TGG 0.475670 3_3:-55903258 Msa0459400:CDS
ACTTCCAACTTCACTTCAGA+TGG 0.478421 3_3:+55902445 None:intergenic
AGCTTGGCAATTGCAGACTT+GGG 0.487576 3_3:-55903242 Msa0459400:CDS
TTGAGGGGCTTGTGCTCGTA+AGG 0.492643 3_3:+55902789 None:intergenic
TAAGCCTTGATGACATTTGA+GGG 0.496394 3_3:+55902773 None:intergenic
ATTCCTCGTACAGTCTTCAA+TGG 0.498924 3_3:-55902944 Msa0459400:CDS
TTAGCTAGGGAATGTTATGA+TGG 0.499854 3_3:-55902034 Msa0459400:three_prime_UTR
ATGGAGATGGATAATAGTTA+GGG 0.500415 3_3:+55902464 None:intergenic
TTGTTACATTTGGGAACTCA+TGG 0.503339 3_3:-55902624 Msa0459400:CDS
ATAACCAGTCTATATTATAT+GGG 0.508466 3_3:-55903374 Msa0459400:CDS
GTAAGCCTTGATGACATTTG+AGG 0.508975 3_3:+55902772 None:intergenic
CGTGCCCAAGAATTAGTTGA+AGG 0.512292 3_3:-55902707 Msa0459400:CDS
GAATGTGTTGCAATATAGGT+TGG 0.528201 3_3:+55903271 None:intergenic
TTTCTCTTTGAAGGTGTCAA+GGG 0.532238 3_3:+55903070 None:intergenic
TAGCTAGGGAATGTTATGAT+GGG 0.540129 3_3:-55902033 Msa0459400:three_prime_UTR
GTTTCTCTTTGAAGGTGTCA+AGG 0.541705 3_3:+55903069 None:intergenic
CATTTAAGATTCAAAGTCAA+AGG 0.545048 3_3:+55902345 None:intergenic
AACTTCACTTCAGATGGAGA+TGG 0.545461 3_3:+55902451 None:intergenic
ATCAGAAATTATCAAAGGTG+TGG 0.551256 3_3:-55903186 Msa0459400:CDS
GTTATGGCTTCGTCTCTCAA+AGG 0.551828 3_3:+55903293 None:intergenic
CTATATTGCAACACATTCCC+CGG 0.563925 3_3:-55903266 Msa0459400:CDS
GTCAGTGACAGTGATTAGCT+AGG 0.568400 3_3:-55902048 Msa0459400:three_prime_UTR
AAGTTGGAAGTTCTCAATGA+AGG 0.574034 3_3:-55902433 Msa0459400:CDS
AGATCCCATATAATATAGAC+TGG 0.575339 3_3:+55903370 None:intergenic
CTGCAATTGCCAAGCTTCCG+GGG 0.575841 3_3:+55903249 None:intergenic
GGCACAATGCATTAGGGCTG+TGG 0.579915 3_3:-55902290 Msa0459400:three_prime_UTR
AAGCCCCTCAAATGTCATCA+AGG 0.583515 3_3:-55902777 Msa0459400:CDS
ATTTGTCATCAATAAACTGG+AGG 0.586227 3_3:-55902407 Msa0459400:CDS
AGCTAGGGAATGTTATGATG+GGG 0.592444 3_3:-55902032 Msa0459400:three_prime_UTR
CCAAAGTGACTAACTAGCAA+AGG 0.593693 3_3:+55902262 None:intergenic
TCAGTGACAGTGATTAGCTA+GGG 0.595013 3_3:-55902047 Msa0459400:three_prime_UTR
GTGATATCAGAAATTATCAA+AGG 0.602157 3_3:-55903191 Msa0459400:CDS
AATTGTAAATACGCAGCGAA+AGG 0.609362 3_3:-55903333 Msa0459400:CDS
GGTGATTCATTAGGTTCCAG+AGG 0.617653 3_3:-55902840 Msa0459400:intron
GCTAATCACTGTCACTGACA+AGG 0.625900 3_3:+55902052 None:intergenic
ATGCGACAATCCATCAAGCA+AGG 0.626620 3_3:-55902651 Msa0459400:CDS
AAGCCTTGATGACATTTGAG+GGG 0.639370 3_3:+55902774 None:intergenic
GTGATTCATTAGGTTCCAGA+GGG 0.640011 3_3:-55902839 Msa0459400:intron
ATTCTGCCATAAAATGAACC+AGG 0.644081 3_3:+55902996 None:intergenic
GGAACCCCATAGATGTAACA+CGG 0.651299 3_3:+55903017 None:intergenic
GTATGTTGAAATTATCTCTG+AGG 0.653657 3_3:-55901943 Msa0459400:three_prime_UTR
GAACCCCATAGATGTAACAC+GGG 0.684498 3_3:+55903018 None:intergenic
GAGGGTGCTGACAACAACAA+AGG 0.692851 3_3:-55902821 Msa0459400:CDS
TAGCCATTGAAGACTGTACG+AGG 0.694298 3_3:+55902941 None:intergenic
TCTCTGAGGAGTTTAATCAG+TGG 0.702542 3_3:-55901929 Msa0459400:three_prime_UTR
AGGCCCGTGTTACATCTATG+GGG 0.761192 3_3:-55903021 Msa0459400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCAAAAATACTTTATATT+CGG + chr3_3:55902261-55902280 None:intergenic 10.0%
!! ATTCAAAAATACTTTATATT+CGG + chr3_3:55902261-55902280 None:intergenic 10.0%
!!! ATTTTCTCTATATTTAGTAA+AGG - chr3_3:55901955-55901974 Msa0459400:three_prime_UTR 15.0%
!! TTCAAAAATACTTTATATTC+GGG + chr3_3:55902260-55902279 None:intergenic 15.0%
!!! ATAGAAAAATTTTAACTGAT+CGG - chr3_3:55903181-55903200 Msa0459400:CDS 15.0%
!! ATAACTAACAACATATATAT+TGG + chr3_3:55903401-55903420 None:intergenic 15.0%
!!! ATTTTCTCTATATTTAGTAA+AGG - chr3_3:55901955-55901974 Msa0459400:three_prime_UTR 15.0%
!! TTCAAAAATACTTTATATTC+GGG + chr3_3:55902260-55902279 None:intergenic 15.0%
!!! ATAGAAAAATTTTAACTGAT+CGG - chr3_3:55903181-55903200 Msa0459400:CDS 15.0%
!! ATAACTAACAACATATATAT+TGG + chr3_3:55903401-55903420 None:intergenic 15.0%
!! TATAACCAGTCTATATTATA+TGG - chr3_3:55902013-55902032 Msa0459400:three_prime_UTR 20.0%
!! ATAACCAGTCTATATTATAT+GGG - chr3_3:55902014-55902033 Msa0459400:three_prime_UTR 20.0%
!!! TAGATATGAATATTTTGTGT+TGG - chr3_3:55902523-55902542 Msa0459400:intron 20.0%
!!! CAGTTAAAATTTTTCTATAG+CGG + chr3_3:55903179-55903198 None:intergenic 20.0%
!! TATAACCAGTCTATATTATA+TGG - chr3_3:55902013-55902032 Msa0459400:three_prime_UTR 20.0%
!! ATAACCAGTCTATATTATAT+GGG - chr3_3:55902014-55902033 Msa0459400:three_prime_UTR 20.0%
!!! TAGATATGAATATTTTGTGT+TGG - chr3_3:55902523-55902542 Msa0459400:intron 20.0%
!!! CAGTTAAAATTTTTCTATAG+CGG + chr3_3:55903179-55903198 None:intergenic 20.0%
! GTGATATCAGAAATTATCAA+AGG - chr3_3:55902197-55902216 Msa0459400:three_prime_UTR 25.0%
!!! AGTATTTTTGAATGATCTAC+CGG - chr3_3:55902268-55902287 Msa0459400:three_prime_UTR 25.0%
! ACAAAATGCAAACTATTATC+TGG + chr3_3:55902422-55902441 None:intergenic 25.0%
! TTTAATCTACTCATGAAGAA+TGG - chr3_3:55902499-55902518 Msa0459400:CDS 25.0%
!! TATGAATATTTTGTGTTGGA+TGG - chr3_3:55902527-55902546 Msa0459400:intron 25.0%
!!! CAGATTTCTCTTTTTTTCTT+CGG - chr3_3:55902655-55902674 Msa0459400:CDS 25.0%
!! CAAATGATTCTAACACTTTT+AGG - chr3_3:55902708-55902727 Msa0459400:CDS 25.0%
! ATCATTTGTCATCAATAAAC+TGG - chr3_3:55902978-55902997 Msa0459400:CDS 25.0%
!! CATTTAAGATTCAAAGTCAA+AGG + chr3_3:55903046-55903065 None:intergenic 25.0%
! GTGATATCAGAAATTATCAA+AGG - chr3_3:55902197-55902216 Msa0459400:three_prime_UTR 25.0%
!!! AGTATTTTTGAATGATCTAC+CGG - chr3_3:55902268-55902287 Msa0459400:three_prime_UTR 25.0%
! ACAAAATGCAAACTATTATC+TGG + chr3_3:55902422-55902441 None:intergenic 25.0%
! TTTAATCTACTCATGAAGAA+TGG - chr3_3:55902499-55902518 Msa0459400:CDS 25.0%
!! TATGAATATTTTGTGTTGGA+TGG - chr3_3:55902527-55902546 Msa0459400:intron 25.0%
!!! CAGATTTCTCTTTTTTTCTT+CGG - chr3_3:55902655-55902674 Msa0459400:CDS 25.0%
!! CAAATGATTCTAACACTTTT+AGG - chr3_3:55902708-55902727 Msa0459400:CDS 25.0%
! ATCATTTGTCATCAATAAAC+TGG - chr3_3:55902978-55902997 Msa0459400:CDS 25.0%
!! CATTTAAGATTCAAAGTCAA+AGG + chr3_3:55903046-55903065 None:intergenic 25.0%
AGATCCCATATAATATAGAC+TGG + chr3_3:55902021-55902040 None:intergenic 30.0%
ATCAGAAATTATCAAAGGTG+TGG - chr3_3:55902202-55902221 Msa0459400:three_prime_UTR 30.0%
!! GTATTTTTGAATGATCTACC+GGG - chr3_3:55902269-55902288 Msa0459400:three_prime_UTR 30.0%
ATCACATAGTTTCTCTTTGA+AGG + chr3_3:55902330-55902349 None:intergenic 30.0%
AGAGAAACTATGTGATGAAA+TGG - chr3_3:55902334-55902353 Msa0459400:exon 30.0%
GATGAAATGGAAACTGAAAT+AGG - chr3_3:55902347-55902366 Msa0459400:exon 30.0%
!! TGATTCTAACACTTTTAGGA+AGG - chr3_3:55902712-55902731 Msa0459400:CDS 30.0%
!! CTCTAAGACCATATCATTAA+GGG + chr3_3:55902796-55902815 None:intergenic 30.0%
ATGGAGATGGATAATAGTTA+GGG + chr3_3:55902927-55902946 None:intergenic 30.0%
ATTTGTCATCAATAAACTGG+AGG - chr3_3:55902981-55903000 Msa0459400:CDS 30.0%
!! TAGAGATGATGGATACAATA+TGG - chr3_3:55903077-55903096 Msa0459400:CDS 30.0%
! ACCATGAGTAGAAACATTTT+TGG - chr3_3:55903257-55903276 Msa0459400:CDS 30.0%
! ACCAAAAATGTTTCTACTCA+TGG + chr3_3:55903261-55903280 None:intergenic 30.0%
GTATGTTGAAATTATCTCTG+AGG - chr3_3:55903445-55903464 Msa0459400:five_prime_UTR 30.0%
AGATCCCATATAATATAGAC+TGG + chr3_3:55902021-55902040 None:intergenic 30.0%
ATCAGAAATTATCAAAGGTG+TGG - chr3_3:55902202-55902221 Msa0459400:three_prime_UTR 30.0%
!! GTATTTTTGAATGATCTACC+GGG - chr3_3:55902269-55902288 Msa0459400:three_prime_UTR 30.0%
ATCACATAGTTTCTCTTTGA+AGG + chr3_3:55902330-55902349 None:intergenic 30.0%
AGAGAAACTATGTGATGAAA+TGG - chr3_3:55902334-55902353 Msa0459400:exon 30.0%
GATGAAATGGAAACTGAAAT+AGG - chr3_3:55902347-55902366 Msa0459400:exon 30.0%
!! TGATTCTAACACTTTTAGGA+AGG - chr3_3:55902712-55902731 Msa0459400:CDS 30.0%
!! CTCTAAGACCATATCATTAA+GGG + chr3_3:55902796-55902815 None:intergenic 30.0%
ATGGAGATGGATAATAGTTA+GGG + chr3_3:55902927-55902946 None:intergenic 30.0%
ATTTGTCATCAATAAACTGG+AGG - chr3_3:55902981-55903000 Msa0459400:CDS 30.0%
!! TAGAGATGATGGATACAATA+TGG - chr3_3:55903077-55903096 Msa0459400:CDS 30.0%
! ACCATGAGTAGAAACATTTT+TGG - chr3_3:55903257-55903276 Msa0459400:CDS 30.0%
! ACCAAAAATGTTTCTACTCA+TGG + chr3_3:55903261-55903280 None:intergenic 30.0%
GTATGTTGAAATTATCTCTG+AGG - chr3_3:55903445-55903464 Msa0459400:five_prime_UTR 30.0%
AATTGTAAATACGCAGCGAA+AGG - chr3_3:55902055-55902074 Msa0459400:three_prime_UTR 35.0%
!! GTTGCAATATAGGTTGGTTA+TGG + chr3_3:55902114-55902133 None:intergenic 35.0%
GAATGTGTTGCAATATAGGT+TGG + chr3_3:55902120-55902139 None:intergenic 35.0%
! TTTTGGACCAAACACTTTGT+TGG - chr3_3:55902175-55902194 Msa0459400:three_prime_UTR 35.0%
! GATATCACCAACAAAGTGTT+TGG + chr3_3:55902185-55902204 None:intergenic 35.0%
!! TTTCTCTTTGAAGGTGTCAA+GGG + chr3_3:55902321-55902340 None:intergenic 35.0%
ATTCTGCCATAAAATGAACC+AGG + chr3_3:55902395-55902414 None:intergenic 35.0%
! TAAGCCTTGATGACATTTGA+GGG + chr3_3:55902618-55902637 None:intergenic 35.0%
AGCACCTTCAACTAATTCTT+GGG + chr3_3:55902688-55902707 None:intergenic 35.0%
!! CAAGGAGTGTTGTTACATTT+GGG - chr3_3:55902755-55902774 Msa0459400:CDS 35.0%
TTGTTACATTTGGGAACTCA+TGG - chr3_3:55902764-55902783 Msa0459400:CDS 35.0%
!! CCTCTAAGACCATATCATTA+AGG + chr3_3:55902797-55902816 None:intergenic 35.0%
! CCTTAATGATATGGTCTTAG+AGG - chr3_3:55902794-55902813 Msa0459400:CDS 35.0%
AATAAATCGATCTATGCTGC+AGG - chr3_3:55902864-55902883 Msa0459400:intron 35.0%
ATAAATCGATCTATGCTGCA+GGG - chr3_3:55902865-55902884 Msa0459400:intron 35.0%
GATGGAGATGGATAATAGTT+AGG + chr3_3:55902928-55902947 None:intergenic 35.0%
AAGTTGGAAGTTCTCAATGA+AGG - chr3_3:55902955-55902974 Msa0459400:CDS 35.0%
!! TGGAGGTTTCTGAAGTAAAT+TGG - chr3_3:55902998-55903017 Msa0459400:CDS 35.0%
! ATTGGAATGCTCTTGATGAT+TGG - chr3_3:55903016-55903035 Msa0459400:CDS 35.0%
TCAGAATCACTTAGAGATGA+TGG - chr3_3:55903066-55903085 Msa0459400:CDS 35.0%
ACAATATGGCACAATGCATT+AGG - chr3_3:55903091-55903110 Msa0459400:CDS 35.0%
CAATATGGCACAATGCATTA+GGG - chr3_3:55903092-55903111 Msa0459400:CDS 35.0%
TGTCAACGATATGCTAAGAT+TGG + chr3_3:55903232-55903251 None:intergenic 35.0%
TTAGCTAGGGAATGTTATGA+TGG - chr3_3:55903354-55903373 Msa0459400:CDS 35.0%
TAGCTAGGGAATGTTATGAT+GGG - chr3_3:55903355-55903374 Msa0459400:CDS 35.0%
AATTGTAAATACGCAGCGAA+AGG - chr3_3:55902055-55902074 Msa0459400:three_prime_UTR 35.0%
!! GTTGCAATATAGGTTGGTTA+TGG + chr3_3:55902114-55902133 None:intergenic 35.0%
GAATGTGTTGCAATATAGGT+TGG + chr3_3:55902120-55902139 None:intergenic 35.0%
! TTTTGGACCAAACACTTTGT+TGG - chr3_3:55902175-55902194 Msa0459400:three_prime_UTR 35.0%
! GATATCACCAACAAAGTGTT+TGG + chr3_3:55902185-55902204 None:intergenic 35.0%
!! TTTCTCTTTGAAGGTGTCAA+GGG + chr3_3:55902321-55902340 None:intergenic 35.0%
ATTCTGCCATAAAATGAACC+AGG + chr3_3:55902395-55902414 None:intergenic 35.0%
! TAAGCCTTGATGACATTTGA+GGG + chr3_3:55902618-55902637 None:intergenic 35.0%
AGCACCTTCAACTAATTCTT+GGG + chr3_3:55902688-55902707 None:intergenic 35.0%
!! CAAGGAGTGTTGTTACATTT+GGG - chr3_3:55902755-55902774 Msa0459400:CDS 35.0%
TTGTTACATTTGGGAACTCA+TGG - chr3_3:55902764-55902783 Msa0459400:CDS 35.0%
!! CCTCTAAGACCATATCATTA+AGG + chr3_3:55902797-55902816 None:intergenic 35.0%
! CCTTAATGATATGGTCTTAG+AGG - chr3_3:55902794-55902813 Msa0459400:CDS 35.0%
AATAAATCGATCTATGCTGC+AGG - chr3_3:55902864-55902883 Msa0459400:intron 35.0%
ATAAATCGATCTATGCTGCA+GGG - chr3_3:55902865-55902884 Msa0459400:intron 35.0%
GATGGAGATGGATAATAGTT+AGG + chr3_3:55902928-55902947 None:intergenic 35.0%
AAGTTGGAAGTTCTCAATGA+AGG - chr3_3:55902955-55902974 Msa0459400:CDS 35.0%
!! TGGAGGTTTCTGAAGTAAAT+TGG - chr3_3:55902998-55903017 Msa0459400:CDS 35.0%
! ATTGGAATGCTCTTGATGAT+TGG - chr3_3:55903016-55903035 Msa0459400:CDS 35.0%
TCAGAATCACTTAGAGATGA+TGG - chr3_3:55903066-55903085 Msa0459400:CDS 35.0%
ACAATATGGCACAATGCATT+AGG - chr3_3:55903091-55903110 Msa0459400:CDS 35.0%
CAATATGGCACAATGCATTA+GGG - chr3_3:55903092-55903111 Msa0459400:CDS 35.0%
TGTCAACGATATGCTAAGAT+TGG + chr3_3:55903232-55903251 None:intergenic 35.0%
TTAGCTAGGGAATGTTATGA+TGG - chr3_3:55903354-55903373 Msa0459400:CDS 35.0%
TAGCTAGGGAATGTTATGAT+GGG - chr3_3:55903355-55903374 Msa0459400:CDS 35.0%
CTATATTGCAACACATTCCC+CGG - chr3_3:55902122-55902141 Msa0459400:three_prime_UTR 40.0%
ACGTTGTTGAAGTCATTTCC+CGG + chr3_3:55902290-55902309 None:intergenic 40.0%
!! GTTTCTCTTTGAAGGTGTCA+AGG + chr3_3:55902322-55902341 None:intergenic 40.0%
ATTCCTCGTACAGTCTTCAA+TGG - chr3_3:55902444-55902463 Msa0459400:CDS 40.0%
CAGTCTTCAATGGCTATCTA+AGG - chr3_3:55902454-55902473 Msa0459400:CDS 40.0%
GTGTTGGATGGTGATTCATT+AGG - chr3_3:55902539-55902558 Msa0459400:intron 40.0%
GTGATTCATTAGGTTCCAGA+GGG - chr3_3:55902549-55902568 Msa0459400:intron 40.0%
! AAGCCTTGATGACATTTGAG+GGG + chr3_3:55902617-55902636 None:intergenic 40.0%
! GTAAGCCTTGATGACATTTG+AGG + chr3_3:55902619-55902638 None:intergenic 40.0%
GAGCACCTTCAACTAATTCT+TGG + chr3_3:55902689-55902708 None:intergenic 40.0%
TAACAACACTCCTTGCTTGA+TGG + chr3_3:55902750-55902769 None:intergenic 40.0%
!! GCAAGGAGTGTTGTTACATT+TGG - chr3_3:55902754-55902773 Msa0459400:CDS 40.0%
GGCTATCTCCCTTAATGATA+TGG - chr3_3:55902785-55902804 Msa0459400:CDS 40.0%
AACTTCACTTCAGATGGAGA+TGG + chr3_3:55902940-55902959 None:intergenic 40.0%
! ATCTCCATCTGAAGTGAAGT+TGG - chr3_3:55902939-55902958 Msa0459400:CDS 40.0%
ACTTCCAACTTCACTTCAGA+TGG + chr3_3:55902946-55902965 None:intergenic 40.0%
CCAAAGTGACTAACTAGCAA+AGG + chr3_3:55903129-55903148 None:intergenic 40.0%
CCTTTGCTAGTTAGTCACTT+TGG - chr3_3:55903126-55903145 Msa0459400:CDS 40.0%
! CTAGTTAGTCACTTTGGTGA+AGG - chr3_3:55903132-55903151 Msa0459400:CDS 40.0%
!! CTTTGGTGAAGGTGTCATTA+AGG - chr3_3:55903143-55903162 Msa0459400:CDS 40.0%
! GGTGAACTTAGTCTTCTCTT+TGG + chr3_3:55903211-55903230 None:intergenic 40.0%
! TCAGTGACAGTGATTAGCTA+GGG - chr3_3:55903341-55903360 Msa0459400:CDS 40.0%
AGCTAGGGAATGTTATGATG+GGG - chr3_3:55903356-55903375 Msa0459400:CDS 40.0%
! TCTCTGAGGAGTTTAATCAG+TGG - chr3_3:55903459-55903478 Msa0459400:five_prime_UTR 40.0%
CTATATTGCAACACATTCCC+CGG - chr3_3:55902122-55902141 Msa0459400:three_prime_UTR 40.0%
ACGTTGTTGAAGTCATTTCC+CGG + chr3_3:55902290-55902309 None:intergenic 40.0%
!! GTTTCTCTTTGAAGGTGTCA+AGG + chr3_3:55902322-55902341 None:intergenic 40.0%
ATTCCTCGTACAGTCTTCAA+TGG - chr3_3:55902444-55902463 Msa0459400:CDS 40.0%
CAGTCTTCAATGGCTATCTA+AGG - chr3_3:55902454-55902473 Msa0459400:CDS 40.0%
GTGTTGGATGGTGATTCATT+AGG - chr3_3:55902539-55902558 Msa0459400:intron 40.0%
GTGATTCATTAGGTTCCAGA+GGG - chr3_3:55902549-55902568 Msa0459400:intron 40.0%
! AAGCCTTGATGACATTTGAG+GGG + chr3_3:55902617-55902636 None:intergenic 40.0%
! GTAAGCCTTGATGACATTTG+AGG + chr3_3:55902619-55902638 None:intergenic 40.0%
GAGCACCTTCAACTAATTCT+TGG + chr3_3:55902689-55902708 None:intergenic 40.0%
TAACAACACTCCTTGCTTGA+TGG + chr3_3:55902750-55902769 None:intergenic 40.0%
!! GCAAGGAGTGTTGTTACATT+TGG - chr3_3:55902754-55902773 Msa0459400:CDS 40.0%
GGCTATCTCCCTTAATGATA+TGG - chr3_3:55902785-55902804 Msa0459400:CDS 40.0%
AACTTCACTTCAGATGGAGA+TGG + chr3_3:55902940-55902959 None:intergenic 40.0%
! ATCTCCATCTGAAGTGAAGT+TGG - chr3_3:55902939-55902958 Msa0459400:CDS 40.0%
ACTTCCAACTTCACTTCAGA+TGG + chr3_3:55902946-55902965 None:intergenic 40.0%
CCAAAGTGACTAACTAGCAA+AGG + chr3_3:55903129-55903148 None:intergenic 40.0%
CCTTTGCTAGTTAGTCACTT+TGG - chr3_3:55903126-55903145 Msa0459400:CDS 40.0%
! CTAGTTAGTCACTTTGGTGA+AGG - chr3_3:55903132-55903151 Msa0459400:CDS 40.0%
!! CTTTGGTGAAGGTGTCATTA+AGG - chr3_3:55903143-55903162 Msa0459400:CDS 40.0%
! GGTGAACTTAGTCTTCTCTT+TGG + chr3_3:55903211-55903230 None:intergenic 40.0%
! TCAGTGACAGTGATTAGCTA+GGG - chr3_3:55903341-55903360 Msa0459400:CDS 40.0%
AGCTAGGGAATGTTATGATG+GGG - chr3_3:55903356-55903375 Msa0459400:CDS 40.0%
! TCTCTGAGGAGTTTAATCAG+TGG - chr3_3:55903459-55903478 Msa0459400:five_prime_UTR 40.0%
GTTATGGCTTCGTCTCTCAA+AGG + chr3_3:55902098-55902117 None:intergenic 45.0%
CGGGGAATGTGTTGCAATAT+AGG + chr3_3:55902124-55902143 None:intergenic 45.0%
AAGCTTGGCAATTGCAGACT+TGG - chr3_3:55902145-55902164 Msa0459400:three_prime_UTR 45.0%
AGCTTGGCAATTGCAGACTT+GGG - chr3_3:55902146-55902165 Msa0459400:three_prime_UTR 45.0%
ATAGGCCCGTGTTACATCTA+TGG - chr3_3:55902365-55902384 Msa0459400:CDS 45.0%
TAGGCCCGTGTTACATCTAT+GGG - chr3_3:55902366-55902385 Msa0459400:CDS 45.0%
GAACCCCATAGATGTAACAC+GGG + chr3_3:55902373-55902392 None:intergenic 45.0%
GGAACCCCATAGATGTAACA+CGG + chr3_3:55902374-55902393 None:intergenic 45.0%
TGTTACATCTATGGGGTTCC+TGG - chr3_3:55902374-55902393 Msa0459400:CDS 45.0%
! GGGGTTCCTGGTTCATTTTA+TGG - chr3_3:55902386-55902405 Msa0459400:CDS 45.0%
TAGCCATTGAAGACTGTACG+AGG + chr3_3:55902450-55902469 None:intergenic 45.0%
GGTGATTCATTAGGTTCCAG+AGG - chr3_3:55902548-55902567 Msa0459400:intron 45.0%
AAGCCCCTCAAATGTCATCA+AGG - chr3_3:55902611-55902630 Msa0459400:CDS 45.0%
CGTGCCCAAGAATTAGTTGA+AGG - chr3_3:55902681-55902700 Msa0459400:CDS 45.0%
ATGCGACAATCCATCAAGCA+AGG - chr3_3:55902737-55902756 Msa0459400:CDS 45.0%
GCTAATCACTGTCACTGACA+AGG + chr3_3:55903339-55903358 None:intergenic 45.0%
! GTCAGTGACAGTGATTAGCT+AGG - chr3_3:55903340-55903359 Msa0459400:CDS 45.0%
GTTATGGCTTCGTCTCTCAA+AGG + chr3_3:55902098-55902117 None:intergenic 45.0%
CGGGGAATGTGTTGCAATAT+AGG + chr3_3:55902124-55902143 None:intergenic 45.0%
AAGCTTGGCAATTGCAGACT+TGG - chr3_3:55902145-55902164 Msa0459400:three_prime_UTR 45.0%
AGCTTGGCAATTGCAGACTT+GGG - chr3_3:55902146-55902165 Msa0459400:three_prime_UTR 45.0%
ATAGGCCCGTGTTACATCTA+TGG - chr3_3:55902365-55902384 Msa0459400:CDS 45.0%
TAGGCCCGTGTTACATCTAT+GGG - chr3_3:55902366-55902385 Msa0459400:CDS 45.0%
GAACCCCATAGATGTAACAC+GGG + chr3_3:55902373-55902392 None:intergenic 45.0%
GGAACCCCATAGATGTAACA+CGG + chr3_3:55902374-55902393 None:intergenic 45.0%
TGTTACATCTATGGGGTTCC+TGG - chr3_3:55902374-55902393 Msa0459400:CDS 45.0%
! GGGGTTCCTGGTTCATTTTA+TGG - chr3_3:55902386-55902405 Msa0459400:CDS 45.0%
TAGCCATTGAAGACTGTACG+AGG + chr3_3:55902450-55902469 None:intergenic 45.0%
GGTGATTCATTAGGTTCCAG+AGG - chr3_3:55902548-55902567 Msa0459400:intron 45.0%
AAGCCCCTCAAATGTCATCA+AGG - chr3_3:55902611-55902630 Msa0459400:CDS 45.0%
CGTGCCCAAGAATTAGTTGA+AGG - chr3_3:55902681-55902700 Msa0459400:CDS 45.0%
ATGCGACAATCCATCAAGCA+AGG - chr3_3:55902737-55902756 Msa0459400:CDS 45.0%
GCTAATCACTGTCACTGACA+AGG + chr3_3:55903339-55903358 None:intergenic 45.0%
! GTCAGTGACAGTGATTAGCT+AGG - chr3_3:55903340-55903359 Msa0459400:CDS 45.0%
TCTGCAATTGCCAAGCTTCC+GGG + chr3_3:55902143-55902162 None:intergenic 50.0%
GTCTGCAATTGCCAAGCTTC+CGG + chr3_3:55902144-55902163 None:intergenic 50.0%
!! GCAGACTTGGGTTGCTCTTT+TGG - chr3_3:55902158-55902177 Msa0459400:three_prime_UTR 50.0%
AGGCCCGTGTTACATCTATG+GGG - chr3_3:55902367-55902386 Msa0459400:CDS 50.0%
! TTGTTGTTGTCAGCACCCTC+TGG + chr3_3:55902567-55902586 None:intergenic 50.0%
!! GAGGGTGCTGACAACAACAA+AGG - chr3_3:55902567-55902586 Msa0459400:intron 50.0%
TCTGCAATTGCCAAGCTTCC+GGG + chr3_3:55902143-55902162 None:intergenic 50.0%
GTCTGCAATTGCCAAGCTTC+CGG + chr3_3:55902144-55902163 None:intergenic 50.0%
!! GCAGACTTGGGTTGCTCTTT+TGG - chr3_3:55902158-55902177 Msa0459400:three_prime_UTR 50.0%
AGGCCCGTGTTACATCTATG+GGG - chr3_3:55902367-55902386 Msa0459400:CDS 50.0%
! TTGTTGTTGTCAGCACCCTC+TGG + chr3_3:55902567-55902586 None:intergenic 50.0%
!! GAGGGTGCTGACAACAACAA+AGG - chr3_3:55902567-55902586 Msa0459400:intron 50.0%
CAACACATTCCCCGGAAGCT+TGG - chr3_3:55902130-55902149 Msa0459400:three_prime_UTR 55.0%
CTGCAATTGCCAAGCTTCCG+GGG + chr3_3:55902142-55902161 None:intergenic 55.0%
TTGAGGGGCTTGTGCTCGTA+AGG + chr3_3:55902602-55902621 None:intergenic 55.0%
GGCACAATGCATTAGGGCTG+TGG - chr3_3:55903098-55903117 Msa0459400:CDS 55.0%
CAACACATTCCCCGGAAGCT+TGG - chr3_3:55902130-55902149 Msa0459400:three_prime_UTR 55.0%
CTGCAATTGCCAAGCTTCCG+GGG + chr3_3:55902142-55902161 None:intergenic 55.0%
TTGAGGGGCTTGTGCTCGTA+AGG + chr3_3:55902602-55902621 None:intergenic 55.0%
GGCACAATGCATTAGGGCTG+TGG - chr3_3:55903098-55903117 Msa0459400:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 55901926 55903484 55901926 ID=Msa0459400;Name=Msa0459400
chr3_3 mRNA 55901926 55903484 55901926 ID=Msa0459400-mRNA-1;Parent=Msa0459400;Name=Msa0459400-mRNA-1;_AED=0.05;_eAED=0.05;_QI=82|1|1|1|1|1|3|422|299
chr3_3 exon 55901926 55902524 55901926 ID=Msa0459400-mRNA-1:exon:11112;Parent=Msa0459400-mRNA-1
chr3_3 exon 55902595 55902849 55902595 ID=Msa0459400-mRNA-1:exon:11111;Parent=Msa0459400-mRNA-1
chr3_3 exon 55902935 55903484 55902935 ID=Msa0459400-mRNA-1:exon:11110;Parent=Msa0459400-mRNA-1
chr3_3 five_prime_UTR 55903403 55903484 55903403 ID=Msa0459400-mRNA-1:five_prime_utr;Parent=Msa0459400-mRNA-1
chr3_3 CDS 55902935 55903402 55902935 ID=Msa0459400-mRNA-1:cds;Parent=Msa0459400-mRNA-1
chr3_3 CDS 55902595 55902849 55902595 ID=Msa0459400-mRNA-1:cds;Parent=Msa0459400-mRNA-1
chr3_3 CDS 55902348 55902524 55902348 ID=Msa0459400-mRNA-1:cds;Parent=Msa0459400-mRNA-1
chr3_3 three_prime_UTR 55901926 55902347 55901926 ID=Msa0459400-mRNA-1:three_prime_utr;Parent=Msa0459400-mRNA-1
Gene Sequence

>Msa0459400

ATGCATATAACCAGTCTATATTATATGGGATCTGAATTTTATGCATCAATTGTAAATACGCAGCGAAAGGTGATTTCTTTAACAAAACCTTTGAGAGACGAAGCCATAACCAACCTATATTGCAACACATTCCCCGGAAGCTTGGCAATTGCAGACTTGGGTTGCTCTTTTGGACCAAACACTTTGTTGGTGATATCAGAAATTATCAAAGGTGTGGAAAAACTTTGTAAAGTGCATCATCATAAATCTCCCGAATATAAAGTATTTTTGAATGATCTACCGGGAAATGACTTCAACAACGTATTCAAGTCCCTTGACACCTTCAAAGAGAAACTATGTGATGAAATGGAAACTGAAATAGGCCCGTGTTACATCTATGGGGTTCCTGGTTCATTTTATGGCAGAATTTTTCCAGATAATAGTTTGCATTTTGTTCATTCCTCGTACAGTCTTCAATGGCTATCTAAGGTTCCAGAGGGTGCTGACAACAACAAAGGAAACAATTACCTTACGAGCACAAGCCCCTCAAATGTCATCAAGGCTTACTACAAGCAATTTCGAACAGATTTCTCTTTTTTTCTTCGGTGTCGTGCCCAAGAATTAGTTGAAGGTGCTCAAATGATTCTAACACTTTTAGGAAGGAAATGCGACAATCCATCAAGCAAGGAGTGTTGTTACATTTGGGAACTCATGGCTATCTCCCTTAATGATATGGTCTTAGAGGGAATCATTAACGAAGAGAAAATCAATACTTTCAACATCCCTAACTATTATCCATCTCCATCTGAAGTGAAGTTGGAAGTTCTCAATGAAGGATCATTTGTCATCAATAAACTGGAGGTTTCTGAAGTAAATTGGAATGCTCTTGATGATTGGAATGCCTTTGACTTTGAATCTTAA
Protein sequence

>Msa0459400

MHITSLYYMGSEFYASIVNTQRKVISLTKPLRDEAITNLYCNTFPGSLAIADLGCSFGPNTLLVISEIIKGVEKLCKVHHHKSPEYKVFLNDLPGNDFNNVFKSLDTFKEKLCDEMETEIGPCYIYGVPGSFYGRIFPDNSLHFVHSSYSLQWLSKVPEGADNNKGNNYLTSTSPSNVIKAYYKQFRTDFSFFLRCRAQELVEGAQMILTLLGRKCDNPSSKECCYIWELMAISLNDMVLEGIINEEKINTFNIPNYYPSPSEVKLEVLNEGSFVINKLEVSEVNWNALDDWNAFDFES*