AlfalfaGEDB | Msa0902730

M. sativa cultivar zhongmu-4 / Msa0902730

KEGG: K02905
GO: GO:0000003 GO:0000184 GO:0000956 GO:0002181 GO:0003674 GO:0003682 GO:0003735 GO:0005198 GO:0005488 GO:0005539 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005783 GO:0005789 GO:0005791 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0007155 GO:0007275 GO:0007565 GO:0007566 GO:0007568 GO:0008104 GO:0008150 GO:0008152 GO:0008201 GO:0008283 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0012505 GO:0015031 GO:0015833 GO:0015934 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0019538 GO:0019898 GO:0022414 GO:0022610 GO:0022625 GO:0022626 GO:0030867 GO:0031090 GO:0031589 GO:0031974 GO:0031981 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034641 GO:0034645 GO:0034655 GO:0035264 GO:0040007 GO:0042175 GO:0042788 GO:0042886 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0045047 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0048589 GO:0048856 GO:0050789 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0090304 GO:0097367 GO:0098552 GO:0098554 GO:0098556 GO:0098562 GO:0098588 GO:0098827 GO:1901360 GO:1901361 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901681 GO:1990904
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0902730	PNX84403.1	98.333	60	1	0	1	60	1	60	6.16e-34	119

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0902730	sp\|Q9M7X7\|RL291_ARATH	85.000	60	9	0	1	60	1	60	4.91e-30	102

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0902730	A0A2K3M0X4	98.333	60	1	0	1	60	1	60	2.94e-34	119

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0086160	Msa0902730	0.801863	7.364677e-49	-8.615850e-47
Msa0087910	Msa0902730	0.801821	7.515313e-49	-8.615850e-47
Msa0114600	Msa0902730	0.800910	1.154590e-48	-8.615850e-47
Msa0602900	Msa0902730	0.818156	2.270768e-52	-8.615850e-47
Msa0605890	Msa0902730	0.860435	2.070508e-63	-8.615850e-47
Msa0619230	Msa0902730	0.832315	1.014185e-55	-8.615850e-47
Msa0643960	Msa0902730	0.815468	9.102863e-52	-8.615850e-47
Msa0649890	Msa0902730	0.832658	8.334847e-56	-8.615850e-47
Msa0656450	Msa0902730	0.824959	6.082487e-54	-8.615850e-47
Msa0663400	Msa0902730	0.852364	4.846090e-61	-8.615850e-47
Msa0694400	Msa0902730	0.859733	3.371823e-63	-8.615850e-47
Msa0704860	Msa0902730	0.831783	1.372362e-55	-8.615850e-47
Msa0741510	Msa0902730	0.811796	5.853448e-51	-8.615850e-47
Msa0749000	Msa0902730	0.810602	1.062573e-50	-8.615850e-47
Msa0796490	Msa0902730	0.819765	9.778031e-53	-8.615850e-47
Msa0128330	Msa0902730	0.884791	1.417587e-71	-8.615850e-47
Msa0167860	Msa0902730	0.865001	8.100599e-65	-8.615850e-47
Msa0174580	Msa0902730	0.890083	1.356714e-73	-8.615850e-47
Msa0191550	Msa0902730	0.801560	8.500353e-49	-8.615850e-47
Msa0192300	Msa0902730	0.804167	2.459920e-49	-8.615850e-47
Msa0225350	Msa0902730	0.807793	4.249182e-50	-8.615850e-47
Msa0376850	Msa0902730	0.810414	1.166517e-50	-8.615850e-47
Msa0377370	Msa0902730	0.803132	4.033973e-49	-8.615850e-47
Msa0426250	Msa0902730	0.828073	1.101060e-54	-8.615850e-47
Msa0450150	Msa0902730	0.817003	4.129898e-52	-8.615850e-47
Msa0468440	Msa0902730	0.847183	1.359032e-59	-8.615850e-47
Msa0482560	Msa0902730	0.809420	1.908851e-50	-8.615850e-47
Msa0277600	Msa0902730	0.849547	3.015946e-60	-8.615850e-47
Msa0301250	Msa0902730	0.802678	5.005054e-49	-8.615850e-47
Msa0313060	Msa0902730	0.825627	4.228447e-54	-8.615850e-47
Msa0315430	Msa0902730	0.834656	2.642099e-56	-8.615850e-47
Msa0516830	Msa0902730	0.810627	1.049336e-50	-8.615850e-47
Msa0547920	Msa0902730	0.821430	4.053855e-53	-8.615850e-47
Msa0568380	Msa0902730	0.834132	3.576541e-56	-8.615850e-47
Msa0573550	Msa0902730	0.841625	4.243229e-58	-8.615850e-47
Msa0574780	Msa0902730	0.829705	4.432694e-55	-8.615850e-47
Msa0578010	Msa0902730	0.810371	1.191609e-50	-8.615850e-47
Msa0579830	Msa0902730	0.838545	2.698274e-57	-8.615850e-47
Msa0585570	Msa0902730	0.845349	4.293467e-59	-8.615850e-47
Msa0902730	Msa0929700	0.818568	1.831854e-52	-8.615850e-47
Msa0902730	Msa0930890	0.835325	1.792289e-56	-8.615850e-47
Msa0902730	Msa0978470	0.804696	1.908387e-49	-8.615850e-47
Msa0902730	Msa1013430	0.939142	2.088493e-99	-8.615850e-47
Msa0902730	Msa1079910	0.862117	6.358385e-64	-8.615850e-47
Msa0902730	Msa1080390	0.802798	4.728381e-49	-8.615850e-47
Msa0902730	Msa1093500	0.981297	3.010708e-152	-8.615850e-47
Msa0902730	Msa1125900	0.801678	8.038162e-49	-8.615850e-47
Msa0902730	Msa1126100	0.806332	8.659962e-50	-8.615850e-47
Msa0902730	Msa1126690	0.804351	2.252334e-49	-8.615850e-47
Msa0902730	Msa1130680	0.801789	7.628014e-49	-8.615850e-47
Msa0902730	Msa1180000	0.820452	6.809142e-53	-8.615850e-47
Msa0902730	Msa1208330	0.821176	4.640375e-53	-8.615850e-47
Msa0902730	Msa1270640	0.812689	3.737121e-51	-8.615850e-47
Msa0902730	Msa1270710	0.814831	1.261000e-51	-8.615850e-47
Msa0902730	Msa1351020	0.862076	6.547766e-64	-8.615850e-47
Msa0902730	Msa1385480	0.871461	6.714901e-67	-8.615850e-47
Msa0902730	Msa1390760	0.831802	1.357712e-55	-8.615850e-47
Msa0902730	Msa1407550	0.846267	2.418200e-59	-8.615850e-47
Msa0902730	Msa1413570	0.830140	3.473526e-55	-8.615850e-47
Msa0902730	Msa1432310	0.856705	2.684866e-62	-8.615850e-47
Msa0902730	Msa1462580	0.823207	1.568240e-53	-8.615850e-47
Msa0902730	Msa1463570	0.874301	7.515212e-68	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0902730	MtrunA17_Chr4g0068191	98.333	60	1	0	1	60	1	60	5.94e-38	119
Msa0902730	MtrunA17_Chr4g0068181	95.000	60	3	0	1	60	1	60	4.02e-37	117

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0902730	AT3G06680.1	85.000	60	9	1	60	23	82	3.09e-31	103
Msa0902730	AT3G06700.1	85.000	60	9	1	60	1	60	4.99e-31	102
Msa0902730	AT3G06700.2	85.000	60	9	1	60	1	60	4.99e-31	102
Msa0902730	AT3G06700.3	85.000	60	9	1	60	1	60	4.99e-31	102
Msa0902730	AT3G06680.2	85.000	60	9	1	60	1	60	6.78e-31	102
Msa0902730	AT3G06680.3	85.000	60	9	1	60	1	60	6.78e-31	102

Find 32 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCACTGCATCTTCAGAATT+AGG	0.220908	6_1:-102515746	Msa0902730:five_prime_UTR
GAAGTGTGGCGGTGCCTCTT+TGG	0.285127	6_1:+102515637	None:intergenic
TATGTTCGACAGTACTTTGA+TGG	0.292656	6_1:-102515346	Msa0902730:three_prime_UTR
TAATTGTGATTACTTTAGTT+TGG	0.351757	6_1:-102515122	Msa0902730:three_prime_UTR
ATCATAAACAGCAGAATTGA+TGG	0.369582	6_1:+102515218	None:intergenic
TTTATAATTCATGTTATGTA+TGG	0.371005	6_1:-102515382	Msa0902730:three_prime_UTR
GTAAAGTGTCGATCTCTAAT+CGG	0.398848	6_1:-102515429	Msa0902730:exon
TCTGAAGATGCAGTGGCTCT+AGG	0.431928	6_1:+102515752	None:intergenic
AGAATTGATGGTCTAAATTC+AGG	0.442334	6_1:+102515230	None:intergenic
TATGCTTCCGTGCGTACCTC+TGG	0.442911	6_1:+102515483	None:intergenic
ACCGCCACACTTCCACCAAA+GGG	0.460737	6_1:-102515627	Msa0902730:CDS
TGTATGTATATATATCTGTT+GGG	0.473078	6_1:+102515798	None:intergenic
CTGAAGATGCAGTGGCTCTA+GGG	0.484040	6_1:+102515753	None:intergenic
CGCTCACAACCAGTCTTACA+AGG	0.508995	6_1:-102515683	Msa0902730:CDS
ATGATGAATACATACCCCTT+TGG	0.517971	6_1:+102515612	None:intergenic
TAAAGTGTCGATCTCTAATC+GGG	0.518145	6_1:-102515428	Msa0902730:three_prime_UTR
CACCGCCACACTTCCACCAA+AGG	0.519273	6_1:-102515628	Msa0902730:CDS
ATGAATACATACCCCTTTGG+TGG	0.547453	6_1:+102515615	None:intergenic
GTGTATGTATATATATCTGT+TGG	0.583938	6_1:+102515797	None:intergenic
ACCTAATTCTGAAGATGCAG+TGG	0.589479	6_1:+102515745	None:intergenic
GGTTTGTTGAATTTCAGAGA+TGG	0.605195	6_1:-102515725	Msa0902730:exon
ATGGCATCAAGAAGCCAAAG+AGG	0.617235	6_1:-102515651	Msa0902730:CDS
ATACCCCTTTGGTGGAAGTG+TGG	0.622392	6_1:+102515623	None:intergenic
CACACAATCGAAAATGAGTG+TGG	0.630646	6_1:+102515156	None:intergenic
CCCCTTTGGTGGAAGTGTGG+CGG	0.635065	6_1:+102515626	None:intergenic
TGATGCTGAAAGTGAGCTGT+GGG	0.640992	6_1:-102515193	Msa0902730:three_prime_UTR
TTGATGCTGAAAGTGAGCTG+TGG	0.659134	6_1:-102515194	Msa0902730:three_prime_UTR
ACAGATATATATACATACAC+AGG	0.659616	6_1:-102515794	Msa0902730:five_prime_UTR
CCGCCACACTTCCACCAAAG+GGG	0.674155	6_1:-102515626	Msa0902730:intron
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	6_1:+102515684	None:intergenic
TGAGGAACCAGAGGTACGCA+CGG	0.675026	6_1:-102515490	Msa0902730:CDS
GTATGTATATATATCTGTTG+GGG	0.685662	6_1:+102515799	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTATAATTCATGTTATGTA+TGG	-	chr6_1:102515532-102515551	Msa0902730:intron	15.0%
!!	TGTATGTATATATATCTGTT+GGG	+	chr6_1:102515119-102515138	None:intergenic	20.0%
!!	TAATTGTGATTACTTTAGTT+TGG	-	chr6_1:102515792-102515811	Msa0902730:five_prime_UTR	20.0%
!	GTGTATGTATATATATCTGT+TGG	+	chr6_1:102515120-102515139	None:intergenic	25.0%
!	ACAGATATATATACATACAC+AGG	-	chr6_1:102515120-102515139	Msa0902730:three_prime_UTR	25.0%
	TTGTGATTGATTACTGTAGA+TGG	-	chr6_1:102515389-102515408	Msa0902730:three_prime_UTR	30.0%
!	AGAATTGATGGTCTAAATTC+AGG	+	chr6_1:102515687-102515706	None:intergenic	30.0%
	ATCATAAACAGCAGAATTGA+TGG	+	chr6_1:102515699-102515718	None:intergenic	30.0%
!!	GGTTTGTTGAATTTCAGAGA+TGG	-	chr6_1:102515189-102515208	Msa0902730:three_prime_UTR	35.0%
!!!	CTTCTTGATGCCATTTTTGT+GGG	+	chr6_1:102515257-102515276	None:intergenic	35.0%
	ATGATGAATACATACCCCTT+TGG	+	chr6_1:102515305-102515324	None:intergenic	35.0%
!	AGATGGATCCAAAGTTTTTG+AGG	-	chr6_1:102515406-102515425	Msa0902730:three_prime_UTR	35.0%
!	CGGAAGCATAACAAGAAAAA+TGG	-	chr6_1:102515444-102515463	Msa0902730:exon	35.0%
!!	GTAAAGTGTCGATCTCTAAT+CGG	-	chr6_1:102515485-102515504	Msa0902730:CDS	35.0%
!!	TAAAGTGTCGATCTCTAATC+GGG	-	chr6_1:102515486-102515505	Msa0902730:CDS	35.0%
	TATGTTCGACAGTACTTTGA+TGG	-	chr6_1:102515568-102515587	Msa0902730:intron	35.0%
!	TGCAGTACTGTTAGCATTTT+TGG	-	chr6_1:102515611-102515630	Msa0902730:intron	35.0%
!	GCAGTACTGTTAGCATTTTT+GGG	-	chr6_1:102515612-102515631	Msa0902730:intron	35.0%
!	ACCTAATTCTGAAGATGCAG+TGG	+	chr6_1:102515172-102515191	None:intergenic	40.0%
	TCTTACAAGGCCCACAAAAA+TGG	-	chr6_1:102515244-102515263	Msa0902730:three_prime_UTR	40.0%
!!!	GCTTCTTGATGCCATTTTTG+TGG	+	chr6_1:102515258-102515277	None:intergenic	40.0%
	ATGAATACATACCCCTTTGG+TGG	+	chr6_1:102515302-102515321	None:intergenic	40.0%
!	CTCTGGTTCCTCAAAAACTT+TGG	+	chr6_1:102515417-102515436	None:intergenic	40.0%
!!	CAAAGTTTTTGAGGAACCAG+AGG	-	chr6_1:102515415-102515434	Msa0902730:three_prime_UTR	40.0%
	CACACAATCGAAAATGAGTG+TGG	+	chr6_1:102515761-102515780	None:intergenic	40.0%
!!!	AGTGGCTCTAGGGTTTTGTT+CGG	+	chr6_1:102515154-102515173	None:intergenic	45.0%
	GCCACTGCATCTTCAGAATT+AGG	-	chr6_1:102515168-102515187	Msa0902730:three_prime_UTR	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	+	chr6_1:102515233-102515252	None:intergenic	45.0%
!!	TTTTGTGGGCCTTGTAAGAC+TGG	+	chr6_1:102515243-102515262	None:intergenic	45.0%
	ATGGCATCAAGAAGCCAAAG+AGG	-	chr6_1:102515263-102515282	Msa0902730:three_prime_UTR	45.0%
	TTGATGCTGAAAGTGAGCTG+TGG	-	chr6_1:102515720-102515739	Msa0902730:exon	45.0%
	TGATGCTGAAAGTGAGCTGT+GGG	-	chr6_1:102515721-102515740	Msa0902730:exon	45.0%
!	CTGAAGATGCAGTGGCTCTA+GGG	+	chr6_1:102515164-102515183	None:intergenic	50.0%
	TCTGAAGATGCAGTGGCTCT+AGG	+	chr6_1:102515165-102515184	None:intergenic	50.0%
	CGCTCACAACCAGTCTTACA+AGG	-	chr6_1:102515231-102515250	Msa0902730:three_prime_UTR	50.0%
	ATACCCCTTTGGTGGAAGTG+TGG	+	chr6_1:102515294-102515313	None:intergenic	50.0%
!!!	GGCTCTAGGGTTTTGTTCGG+CGG	+	chr6_1:102515151-102515170	None:intergenic	55.0%
	ACCGCCACACTTCCACCAAA+GGG	-	chr6_1:102515287-102515306	Msa0902730:three_prime_UTR	55.0%
!	TGAGGAACCAGAGGTACGCA+CGG	-	chr6_1:102515424-102515443	Msa0902730:three_prime_UTR	55.0%
	TATGCTTCCGTGCGTACCTC+TGG	+	chr6_1:102515434-102515453	None:intergenic	55.0%
	GAAGTGTGGCGGTGCCTCTT+TGG	+	chr6_1:102515280-102515299	None:intergenic	60.0%
	CACCGCCACACTTCCACCAA+AGG	-	chr6_1:102515286-102515305	Msa0902730:three_prime_UTR	60.0%
!	CCCCTTTGGTGGAAGTGTGG+CGG	+	chr6_1:102515291-102515310	None:intergenic	60.0%
	CCGCCACACTTCCACCAAAG+GGG	-	chr6_1:102515288-102515307	Msa0902730:three_prime_UTR	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	102515116	102515820	102515116	ID=Msa0902730;Name=Msa0902730
chr6_1	mRNA	102515116	102515820	102515116	ID=Msa0902730-mRNA-1;Parent=Msa0902730;Name=Msa0902730-mRNA-1;_AED=0.37;_eAED=0.37;_QI=92\|1\|1\|1\|1\|1\|2\|332\|60
chr6_1	exon	102515116	102515528	102515116	ID=Msa0902730-mRNA-1:exon:18028;Parent=Msa0902730-mRNA-1
chr6_1	exon	102515627	102515820	102515627	ID=Msa0902730-mRNA-1:exon:18027;Parent=Msa0902730-mRNA-1
chr6_1	five_prime_UTR	102515729	102515820	102515729	ID=Msa0902730-mRNA-1:five_prime_utr;Parent=Msa0902730-mRNA-1
chr6_1	CDS	102515627	102515728	102515627	ID=Msa0902730-mRNA-1:cds;Parent=Msa0902730-mRNA-1
chr6_1	CDS	102515448	102515528	102515448	ID=Msa0902730-mRNA-1:cds;Parent=Msa0902730-mRNA-1
chr6_1	three_prime_UTR	102515116	102515447	102515116	ID=Msa0902730-mRNA-1:three_prime_utr;Parent=Msa0902730-mRNA-1

Gene Sequence	>Msa0902730 ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCCCACAAAAATGGCATCAAGAAGCCAAAGAGGCACCGCCACACTTCCACCAAAGGGATGGATCCAAAGTTTTTGAGGAACCAGAGGTACGCACGGAAGCATAACAAGAAAAATGGTGAAATTGCAGAAGAAGAGTAA
Protein sequence	>Msa0902730 MAKSKNHTAHNQSYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEIAEEE*