AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0225350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225350 MtrunA17_Chr2g0331701 100.000 94 0 0 1 94 1 94 3.84e-64 188
Msa0225350 MtrunA17_Chr4g0004631 96.809 94 3 0 1 94 1 94 1.09e-62 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225350 AT3G16080.1 88.298 94 11 0 1 94 1 94 7.58e-57 170
Msa0225350 AT1G52300.1 88.298 94 11 0 1 94 1 94 1.80e-56 169
Msa0225350 AT1G15250.2 87.234 94 12 0 1 94 1 94 9.47e-56 167
Msa0225350 AT1G15250.1 87.234 94 12 0 1 94 1 94 9.47e-56 167
Msa0225350 AT3G16080.2 93.333 45 3 0 1 45 1 45 9.96e-25 89.0

Find 49 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAACAAAGATTATCTTAC+TGG 0.230330 2_1:+75478199 None:intergenic
AAACTGAATGTGCATGTAAA+TGG 0.248433 2_1:+75478106 None:intergenic
ATTACTAGTTTGGATTTAAA+TGG 0.272367 2_1:-75478261 Msa0225350:three_prime_UTR
ATTAGTTTGTTGTTTGTGAT+TGG 0.353715 2_1:-75478398 Msa0225350:three_prime_UTR
AATGAAGATAACTGCTCTAA+TGG 0.362725 2_1:+75478052 None:intergenic
AGTGTCGTTCACTAGGGTTT+TGG 0.364773 2_1:-75479063 None:intergenic
CTCTAAAACCGCTCTTGAAT+CGG 0.373242 2_1:+75478613 None:intergenic
GTTGTGTGGCATTACTAGTT+TGG 0.378335 2_1:-75478271 Msa0225350:three_prime_UTR
AAGCGGAGCATCTACTCTTC+TGG 0.402579 2_1:+75478835 None:intergenic
AAACTATTTCATTGTTCAAT+CGG 0.406070 2_1:-75478298 Msa0225350:three_prime_UTR
CTTACCCAGCTGCTCGTGTT+AGG 0.412584 2_1:-75478809 Msa0225350:CDS
TTAGTTTGTTGTTTGTGATT+GGG 0.419657 2_1:-75478397 Msa0225350:three_prime_UTR
TACTGGAGATATTATCATAA+TGG 0.425604 2_1:+75478216 None:intergenic
CATTTCCTAACACGAGCAGC+TGG 0.431351 2_1:+75478804 None:intergenic
TAGTTTGTTGTTTGTGATTG+GGG 0.440836 2_1:-75478396 Msa0225350:three_prime_UTR
ATTTCATTGTTCAATCGGTT+CGG 0.448438 2_1:-75478293 Msa0225350:three_prime_UTR
GAATTGTAGGGTAAGGGAAC+TGG 0.450991 2_1:-75478927 Msa0225350:intron
CGTAGAAAGACTACTGGAAC+TGG 0.453204 2_1:-75478666 Msa0225350:CDS
TTTAGATAACTGGAGTGAAA+AGG 0.467360 2_1:-75478694 Msa0225350:intron
CAGTTCCAGTAGTCTTTCTA+CGG 0.470228 2_1:+75478667 None:intergenic
AGACTCACACACTCTGTGTC+AGG 0.478990 2_1:-75478881 Msa0225350:CDS
AGATAATCTTTGTTATGTAA+AGG 0.486435 2_1:-75478193 Msa0225350:three_prime_UTR
GGTAAGGGAACTGGAAGTTT+CGG 0.493117 2_1:-75478918 Msa0225350:CDS
GAACTGGAAGTTTCGGTAAG+AGG 0.503126 2_1:-75478911 Msa0225350:CDS
AACTGAATGTGCATGTAAAT+GGG 0.508300 2_1:+75478107 None:intergenic
TGAAGCTGCACCTAGGAAGA+AGG 0.512832 2_1:-75478485 Msa0225350:CDS
TGTTGTTTGTGATTGGGGCA+AGG 0.514030 2_1:-75478391 Msa0225350:three_prime_UTR
GTTCAATCGGTTCGGTTGTG+TGG 0.517376 2_1:-75478285 Msa0225350:three_prime_UTR
AGCATCTACTCTTCTGGAGA+TGG 0.532321 2_1:+75478841 None:intergenic
GCAATCCGTAGAAAGACTAC+TGG 0.532707 2_1:-75478672 Msa0225350:CDS
GTGAATGAATTGTAGGGTAA+GGG 0.556316 2_1:-75478933 Msa0225350:intron
GACGCAGCTCCCTTCTTCCT+AGG 0.557312 2_1:+75478475 None:intergenic
AGCAGCTGGGTAAGCACAAG+CGG 0.557462 2_1:+75478818 None:intergenic
GCTTTGTGCATCTTCTACAA+TGG 0.561239 2_1:-75479035 Msa0225350:intron
GAAGCTGCACCTAGGAAGAA+GGG 0.598975 2_1:-75478484 Msa0225350:CDS
TGTGGTTTGATTCTATAACA+TGG 0.604854 2_1:-75478158 Msa0225350:three_prime_UTR
CTACTGGAACTGGTAGGATG+AGG 0.613944 2_1:-75478656 Msa0225350:CDS
CAGGAACTGAAGCTGCACCT+AGG 0.634185 2_1:-75478492 Msa0225350:CDS
ATTTCCTAACACGAGCAGCT+GGG 0.638199 2_1:+75478805 None:intergenic
CACACACTCTGTGTCAGGTG+CGG 0.644929 2_1:-75478876 Msa0225350:CDS
GAAAGACTACTGGAACTGGT+AGG 0.653500 2_1:-75478662 Msa0225350:CDS
AATACCCAACAAAACAACAA+TGG 0.655819 2_1:+75478436 None:intergenic
CTGGAAGTTTCGGTAAGAGG+AGG 0.659557 2_1:-75478908 Msa0225350:CDS
TCTGGAGATGGAAACTGCGG+CGG 0.666717 2_1:+75478853 None:intergenic
TCTTCTGGAGATGGAAACTG+CGG 0.675301 2_1:+75478850 None:intergenic
GTTCCTCGCCGATTCAAGAG+CGG 0.676604 2_1:-75478621 Msa0225350:CDS
AAACCGCTCTTGAATCGGCG+AGG 0.683649 2_1:+75478618 None:intergenic
ACCAAAGTACAAAACCAATG+AGG 0.688571 2_1:+75478344 None:intergenic
TCTATAACATGGATACAGTG+TGG 0.731407 2_1:-75478147 Msa0225350:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTAATTTGATTTGTAATT+AGG - chr2_1:75478330-75478349 Msa0225350:three_prime_UTR 15.0%
!!! GTTAATTTGATTTGTAATTA+GGG - chr2_1:75478331-75478350 Msa0225350:three_prime_UTR 15.0%
!!! TGTTAATTTGATTTGTAATT+AGG - chr2_1:75478330-75478349 Msa0225350:three_prime_UTR 15.0%
!!! GTTAATTTGATTTGTAATTA+GGG - chr2_1:75478331-75478350 Msa0225350:three_prime_UTR 15.0%
!!! GATTATTGATTTTAGATAAC+TGG - chr2_1:75478389-75478408 Msa0225350:three_prime_UTR 20.0%
!! AAACTATTTCATTGTTCAAT+CGG - chr2_1:75478795-75478814 Msa0225350:intron 20.0%
!!! ATTACTAGTTTGGATTTAAA+TGG - chr2_1:75478832-75478851 Msa0225350:CDS 20.0%
!!! AGATAATCTTTGTTATGTAA+AGG - chr2_1:75478900-75478919 Msa0225350:CDS 20.0%
!!! GATTATTGATTTTAGATAAC+TGG - chr2_1:75478389-75478408 Msa0225350:three_prime_UTR 20.0%
!! AAACTATTTCATTGTTCAAT+CGG - chr2_1:75478795-75478814 Msa0225350:intron 20.0%
!!! ATTACTAGTTTGGATTTAAA+TGG - chr2_1:75478832-75478851 Msa0225350:CDS 20.0%
!!! AGATAATCTTTGTTATGTAA+AGG - chr2_1:75478900-75478919 Msa0225350:CDS 20.0%
!! ATTAGATTGATGATAGATGA+AGG + chr2_1:75478099-75478118 None:intergenic 25.0%
!!! GTTGTGGTTTTTTTTAATAC+AGG - chr2_1:75478582-75478601 Msa0225350:intron 25.0%
!!! ATTAGTTTGTTGTTTGTGAT+TGG - chr2_1:75478695-75478714 Msa0225350:intron 25.0%
!!! TTAGTTTGTTGTTTGTGATT+GGG - chr2_1:75478696-75478715 Msa0225350:intron 25.0%
! TACTGGAGATATTATCATAA+TGG + chr2_1:75478880-75478899 None:intergenic 25.0%
! CATAACAAAGATTATCTTAC+TGG + chr2_1:75478897-75478916 None:intergenic 25.0%
!! ATTAGATTGATGATAGATGA+AGG + chr2_1:75478099-75478118 None:intergenic 25.0%
!!! GTTGTGGTTTTTTTTAATAC+AGG - chr2_1:75478582-75478601 Msa0225350:intron 25.0%
!!! ATTAGTTTGTTGTTTGTGAT+TGG - chr2_1:75478695-75478714 Msa0225350:intron 25.0%
!!! TTAGTTTGTTGTTTGTGATT+GGG - chr2_1:75478696-75478715 Msa0225350:intron 25.0%
! TACTGGAGATATTATCATAA+TGG + chr2_1:75478880-75478899 None:intergenic 25.0%
! CATAACAAAGATTATCTTAC+TGG + chr2_1:75478897-75478916 None:intergenic 25.0%
TGTTCATGTGAATGAATTGT+AGG - chr2_1:75478153-75478172 Msa0225350:three_prime_UTR 30.0%
GTTCATGTGAATGAATTGTA+GGG - chr2_1:75478154-75478173 Msa0225350:three_prime_UTR 30.0%
TTTAGATAACTGGAGTGAAA+AGG - chr2_1:75478399-75478418 Msa0225350:three_prime_UTR 30.0%
CCACAACATATCAATGATTA+AGG + chr2_1:75478569-75478588 None:intergenic 30.0%
CCTTAATCATTGATATGTTG+TGG - chr2_1:75478566-75478585 Msa0225350:intron 30.0%
!!! TTTGACCATTGTTGTTTTGT+TGG - chr2_1:75478652-75478671 Msa0225350:CDS 30.0%
!!! TTGACCATTGTTGTTTTGTT+GGG - chr2_1:75478653-75478672 Msa0225350:CDS 30.0%
AATACCCAACAAAACAACAA+TGG + chr2_1:75478660-75478679 None:intergenic 30.0%
!! TAGTTTGTTGTTTGTGATTG+GGG - chr2_1:75478697-75478716 Msa0225350:intron 30.0%
ATTTCATTGTTCAATCGGTT+CGG - chr2_1:75478800-75478819 Msa0225350:intron 30.0%
!! TAAAGGTAGTTTGAGTTTTG+TGG - chr2_1:75478917-75478936 Msa0225350:CDS 30.0%
TGTGGTTTGATTCTATAACA+TGG - chr2_1:75478935-75478954 Msa0225350:intron 30.0%
AACTGAATGTGCATGTAAAT+GGG + chr2_1:75478989-75479008 None:intergenic 30.0%
AAACTGAATGTGCATGTAAA+TGG + chr2_1:75478990-75479009 None:intergenic 30.0%
AATGAAGATAACTGCTCTAA+TGG + chr2_1:75479044-75479063 None:intergenic 30.0%
TGTTCATGTGAATGAATTGT+AGG - chr2_1:75478153-75478172 Msa0225350:three_prime_UTR 30.0%
GTTCATGTGAATGAATTGTA+GGG - chr2_1:75478154-75478173 Msa0225350:three_prime_UTR 30.0%
TTTAGATAACTGGAGTGAAA+AGG - chr2_1:75478399-75478418 Msa0225350:three_prime_UTR 30.0%
CCACAACATATCAATGATTA+AGG + chr2_1:75478569-75478588 None:intergenic 30.0%
CCTTAATCATTGATATGTTG+TGG - chr2_1:75478566-75478585 Msa0225350:intron 30.0%
!!! TTTGACCATTGTTGTTTTGT+TGG - chr2_1:75478652-75478671 Msa0225350:CDS 30.0%
!!! TTGACCATTGTTGTTTTGTT+GGG - chr2_1:75478653-75478672 Msa0225350:CDS 30.0%
AATACCCAACAAAACAACAA+TGG + chr2_1:75478660-75478679 None:intergenic 30.0%
!! TAGTTTGTTGTTTGTGATTG+GGG - chr2_1:75478697-75478716 Msa0225350:intron 30.0%
ATTTCATTGTTCAATCGGTT+CGG - chr2_1:75478800-75478819 Msa0225350:intron 30.0%
!! TAAAGGTAGTTTGAGTTTTG+TGG - chr2_1:75478917-75478936 Msa0225350:CDS 30.0%
TGTGGTTTGATTCTATAACA+TGG - chr2_1:75478935-75478954 Msa0225350:intron 30.0%
AACTGAATGTGCATGTAAAT+GGG + chr2_1:75478989-75479008 None:intergenic 30.0%
AAACTGAATGTGCATGTAAA+TGG + chr2_1:75478990-75479009 None:intergenic 30.0%
AATGAAGATAACTGCTCTAA+TGG + chr2_1:75479044-75479063 None:intergenic 30.0%
! AGATTGATGATAGATGAAGG+AGG + chr2_1:75478096-75478115 None:intergenic 35.0%
TGTGAATGAATTGTAGGGTA+AGG - chr2_1:75478159-75478178 Msa0225350:three_prime_UTR 35.0%
GTGAATGAATTGTAGGGTAA+GGG - chr2_1:75478160-75478179 Msa0225350:three_prime_UTR 35.0%
! TATTGTTTTCACTGCCTCAT+TGG - chr2_1:75478735-75478754 Msa0225350:intron 35.0%
ACCAAAGTACAAAACCAATG+AGG + chr2_1:75478752-75478771 None:intergenic 35.0%
TCTATAACATGGATACAGTG+TGG - chr2_1:75478946-75478965 Msa0225350:intron 35.0%
! AGATTGATGATAGATGAAGG+AGG + chr2_1:75478096-75478115 None:intergenic 35.0%
TGTGAATGAATTGTAGGGTA+AGG - chr2_1:75478159-75478178 Msa0225350:three_prime_UTR 35.0%
GTGAATGAATTGTAGGGTAA+GGG - chr2_1:75478160-75478179 Msa0225350:three_prime_UTR 35.0%
! TATTGTTTTCACTGCCTCAT+TGG - chr2_1:75478735-75478754 Msa0225350:intron 35.0%
ACCAAAGTACAAAACCAATG+AGG + chr2_1:75478752-75478771 None:intergenic 35.0%
TCTATAACATGGATACAGTG+TGG - chr2_1:75478946-75478965 Msa0225350:intron 35.0%
GCTTTGTGCATCTTCTACAA+TGG - chr2_1:75478058-75478077 Msa0225350:three_prime_UTR 40.0%
CTATACGATGTCGTATATGC+TGG - chr2_1:75478119-75478138 Msa0225350:three_prime_UTR 40.0%
CAGTTCCAGTAGTCTTTCTA+CGG + chr2_1:75478429-75478448 None:intergenic 40.0%
!! CTCTAAAACCGCTCTTGAAT+CGG + chr2_1:75478483-75478502 None:intergenic 40.0%
!!! ATTCAAGAGCGGTTTTAGAG+AGG - chr2_1:75478483-75478502 Msa0225350:CDS 40.0%
!!! TTCAAGAGCGGTTTTAGAGA+GGG - chr2_1:75478484-75478503 Msa0225350:CDS 40.0%
!!! GCCTCATTGGTTTTGTACTT+TGG - chr2_1:75478748-75478767 Msa0225350:intron 40.0%
GTTGTGTGGCATTACTAGTT+TGG - chr2_1:75478822-75478841 Msa0225350:CDS 40.0%
GCTTTGTGCATCTTCTACAA+TGG - chr2_1:75478058-75478077 Msa0225350:three_prime_UTR 40.0%
CTATACGATGTCGTATATGC+TGG - chr2_1:75478119-75478138 Msa0225350:three_prime_UTR 40.0%
CAGTTCCAGTAGTCTTTCTA+CGG + chr2_1:75478429-75478448 None:intergenic 40.0%
!! CTCTAAAACCGCTCTTGAAT+CGG + chr2_1:75478483-75478502 None:intergenic 40.0%
!!! ATTCAAGAGCGGTTTTAGAG+AGG - chr2_1:75478483-75478502 Msa0225350:CDS 40.0%
!!! TTCAAGAGCGGTTTTAGAGA+GGG - chr2_1:75478484-75478503 Msa0225350:CDS 40.0%
!!! GCCTCATTGGTTTTGTACTT+TGG - chr2_1:75478748-75478767 Msa0225350:intron 40.0%
GTTGTGTGGCATTACTAGTT+TGG - chr2_1:75478822-75478841 Msa0225350:CDS 40.0%
GAATTGTAGGGTAAGGGAAC+TGG - chr2_1:75478166-75478185 Msa0225350:three_prime_UTR 45.0%
GGTAAGGGAACTGGAAGTTT+CGG - chr2_1:75478175-75478194 Msa0225350:three_prime_UTR 45.0%
GAACTGGAAGTTTCGGTAAG+AGG - chr2_1:75478182-75478201 Msa0225350:three_prime_UTR 45.0%
! TCTTCTGGAGATGGAAACTG+CGG + chr2_1:75478246-75478265 None:intergenic 45.0%
! AGCATCTACTCTTCTGGAGA+TGG + chr2_1:75478255-75478274 None:intergenic 45.0%
ATTTCCTAACACGAGCAGCT+GGG + chr2_1:75478291-75478310 None:intergenic 45.0%
GCAATCCGTAGAAAGACTAC+TGG - chr2_1:75478421-75478440 Msa0225350:three_prime_UTR 45.0%
CGTAGAAAGACTACTGGAAC+TGG - chr2_1:75478427-75478446 Msa0225350:three_prime_UTR 45.0%
GAAAGACTACTGGAACTGGT+AGG - chr2_1:75478431-75478450 Msa0225350:three_prime_UTR 45.0%
! TGTTGTTTGTGATTGGGGCA+AGG - chr2_1:75478702-75478721 Msa0225350:intron 45.0%
GAATTGTAGGGTAAGGGAAC+TGG - chr2_1:75478166-75478185 Msa0225350:three_prime_UTR 45.0%
GGTAAGGGAACTGGAAGTTT+CGG - chr2_1:75478175-75478194 Msa0225350:three_prime_UTR 45.0%
GAACTGGAAGTTTCGGTAAG+AGG - chr2_1:75478182-75478201 Msa0225350:three_prime_UTR 45.0%
! TCTTCTGGAGATGGAAACTG+CGG + chr2_1:75478246-75478265 None:intergenic 45.0%
! AGCATCTACTCTTCTGGAGA+TGG + chr2_1:75478255-75478274 None:intergenic 45.0%
ATTTCCTAACACGAGCAGCT+GGG + chr2_1:75478291-75478310 None:intergenic 45.0%
GCAATCCGTAGAAAGACTAC+TGG - chr2_1:75478421-75478440 Msa0225350:three_prime_UTR 45.0%
CGTAGAAAGACTACTGGAAC+TGG - chr2_1:75478427-75478446 Msa0225350:three_prime_UTR 45.0%
GAAAGACTACTGGAACTGGT+AGG - chr2_1:75478431-75478450 Msa0225350:three_prime_UTR 45.0%
! TGTTGTTTGTGATTGGGGCA+AGG - chr2_1:75478702-75478721 Msa0225350:intron 45.0%
CTGGAAGTTTCGGTAAGAGG+AGG - chr2_1:75478185-75478204 Msa0225350:three_prime_UTR 50.0%
AGACTCACACACTCTGTGTC+AGG - chr2_1:75478212-75478231 Msa0225350:three_prime_UTR 50.0%
! AAGCGGAGCATCTACTCTTC+TGG + chr2_1:75478261-75478280 None:intergenic 50.0%
CATTTCCTAACACGAGCAGC+TGG + chr2_1:75478292-75478311 None:intergenic 50.0%
CTACTGGAACTGGTAGGATG+AGG - chr2_1:75478437-75478456 Msa0225350:three_prime_UTR 50.0%
TGAAGCTGCACCTAGGAAGA+AGG - chr2_1:75478608-75478627 Msa0225350:intron 50.0%
GAAGCTGCACCTAGGAAGAA+GGG - chr2_1:75478609-75478628 Msa0225350:intron 50.0%
!! GTTCAATCGGTTCGGTTGTG+TGG - chr2_1:75478808-75478827 Msa0225350:CDS 50.0%
CTGGAAGTTTCGGTAAGAGG+AGG - chr2_1:75478185-75478204 Msa0225350:three_prime_UTR 50.0%
AGACTCACACACTCTGTGTC+AGG - chr2_1:75478212-75478231 Msa0225350:three_prime_UTR 50.0%
! AAGCGGAGCATCTACTCTTC+TGG + chr2_1:75478261-75478280 None:intergenic 50.0%
CATTTCCTAACACGAGCAGC+TGG + chr2_1:75478292-75478311 None:intergenic 50.0%
CTACTGGAACTGGTAGGATG+AGG - chr2_1:75478437-75478456 Msa0225350:three_prime_UTR 50.0%
TGAAGCTGCACCTAGGAAGA+AGG - chr2_1:75478608-75478627 Msa0225350:intron 50.0%
GAAGCTGCACCTAGGAAGAA+GGG - chr2_1:75478609-75478628 Msa0225350:intron 50.0%
!! GTTCAATCGGTTCGGTTGTG+TGG - chr2_1:75478808-75478827 Msa0225350:CDS 50.0%
CACACACTCTGTGTCAGGTG+CGG - chr2_1:75478217-75478236 Msa0225350:three_prime_UTR 55.0%
TCTGGAGATGGAAACTGCGG+CGG + chr2_1:75478243-75478262 None:intergenic 55.0%
AGCAGCTGGGTAAGCACAAG+CGG + chr2_1:75478278-75478297 None:intergenic 55.0%
CTTACCCAGCTGCTCGTGTT+AGG - chr2_1:75478284-75478303 Msa0225350:three_prime_UTR 55.0%
!! GTTCCTCGCCGATTCAAGAG+CGG - chr2_1:75478472-75478491 Msa0225350:CDS 55.0%
AAACCGCTCTTGAATCGGCG+AGG + chr2_1:75478478-75478497 None:intergenic 55.0%
CAGGAACTGAAGCTGCACCT+AGG - chr2_1:75478601-75478620 Msa0225350:intron 55.0%
CACACACTCTGTGTCAGGTG+CGG - chr2_1:75478217-75478236 Msa0225350:three_prime_UTR 55.0%
TCTGGAGATGGAAACTGCGG+CGG + chr2_1:75478243-75478262 None:intergenic 55.0%
AGCAGCTGGGTAAGCACAAG+CGG + chr2_1:75478278-75478297 None:intergenic 55.0%
CTTACCCAGCTGCTCGTGTT+AGG - chr2_1:75478284-75478303 Msa0225350:three_prime_UTR 55.0%
!! GTTCCTCGCCGATTCAAGAG+CGG - chr2_1:75478472-75478491 Msa0225350:CDS 55.0%
AAACCGCTCTTGAATCGGCG+AGG + chr2_1:75478478-75478497 None:intergenic 55.0%
CAGGAACTGAAGCTGCACCT+AGG - chr2_1:75478601-75478620 Msa0225350:intron 55.0%
GACGCAGCTCCCTTCTTCCT+AGG + chr2_1:75478621-75478640 None:intergenic 60.0%
GACGCAGCTCCCTTCTTCCT+AGG + chr2_1:75478621-75478640 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 75478041 75479074 75478041 ID=Msa0225350;Name=Msa0225350
chr2_1 mRNA 75478041 75479074 75478041 ID=Msa0225350-mRNA-1;Parent=Msa0225350;Name=Msa0225350-mRNA-1;_AED=0.06;_eAED=0.06;_QI=36|1|1|1|1|1|4|427|94
chr2_1 exon 75478041 75478511 75478041 ID=Msa0225350-mRNA-1:exon:22566;Parent=Msa0225350-mRNA-1
chr2_1 exon 75478610 75478711 75478610 ID=Msa0225350-mRNA-1:exon:22565;Parent=Msa0225350-mRNA-1
chr2_1 exon 75478805 75478940 75478805 ID=Msa0225350-mRNA-1:exon:22564;Parent=Msa0225350-mRNA-1
chr2_1 exon 75479036 75479074 75479036 ID=Msa0225350-mRNA-1:exon:22563;Parent=Msa0225350-mRNA-1
chr2_1 five_prime_UTR 75479039 75479074 75479039 ID=Msa0225350-mRNA-1:five_prime_utr;Parent=Msa0225350-mRNA-1
chr2_1 CDS 75479036 75479038 75479036 ID=Msa0225350-mRNA-1:cds;Parent=Msa0225350-mRNA-1
chr2_1 CDS 75478805 75478940 75478805 ID=Msa0225350-mRNA-1:cds;Parent=Msa0225350-mRNA-1
chr2_1 CDS 75478610 75478711 75478610 ID=Msa0225350-mRNA-1:cds;Parent=Msa0225350-mRNA-1
chr2_1 CDS 75478468 75478511 75478468 ID=Msa0225350-mRNA-1:cds;Parent=Msa0225350-mRNA-1
chr2_1 three_prime_UTR 75478041 75478467 75478041 ID=Msa0225350-mRNA-1:three_prime_utr;Parent=Msa0225350-mRNA-1
Gene Sequence

>Msa0225350

ATGGGTAAGGGAACTGGAAGTTTCGGTAAGAGGAGGAACAAGACTCACACACTCTGTGTCAGGTGCGGCCGCCGCAGTTTCCATCTCCAGAAGAGTAGATGCTCCGCTTGTGCTTACCCAGCTGCTCGTGTTAGGAAATATAACTGGAGTGAAAAGGCAATCCGTAGAAAGACTACTGGAACTGGTAGGATGAGGTACTTGCGCAACGTTCCTCGCCGATTCAAGAGCGGTTTTAGAGAGGGAACTGAAGCTGCACCTAGGAAGAAGGGAGCTGCGTCTGCTTAA
Protein sequence

>Msa0225350

MGKGTGSFGKRRNKTHTLCVRCGRRSFHLQKSRCSACAYPAARVRKYNWSEKAIRRKTTGTGRMRYLRNVPRRFKSGFREGTEAAPRKKGAASA*